CN105384850A - Preparation method of fly larva chitin - Google Patents
Preparation method of fly larva chitin Download PDFInfo
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- CN105384850A CN105384850A CN201510904788.3A CN201510904788A CN105384850A CN 105384850 A CN105384850 A CN 105384850A CN 201510904788 A CN201510904788 A CN 201510904788A CN 105384850 A CN105384850 A CN 105384850A
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Abstract
The invention discloses a preparation method of fly larva chitin. According to the preparation method, the residues after edible oil and protein powder are prepared from fly larvae are deeply processed to prepare the fly larva chitin, and thus the fly larva utilization rate is improved. The technology comprises the following steps: removing impurities from fly larvae, drying and grinding fly larvae, carrying out subcritical extraction to remove grease, performing energy-gathered ultrasonic cycling extraction to remove protein and calcium, and finally carrying out centrifugation in a horizontal screw centrifuge to obtain chitin. The provided preparation method has the advantages that the fly larva is taken as the raw material to prepare chitin, the yield is high, the purity of obtained chitin is high, the larva is converted into valuable resource, the chitin output of large scale production is greatly increased, and at the same time, the environment is protected. Furthermore, the equipment and the operation are simple, the preparation conditions are easy to control, and the preparation method is suitable for massive production.
Description
Technical field
The invention belongs to biological technical field, that be specifically related to is a kind of preparation method of flyblow chitin.
Background technology
Chitin (Chitin) has another name called chitin, chitin, chitin, chitin etc.; chemistry by name 1; 4-2-acetylaminohydroxyphenylarsonic acid 2-deoxidation-callose; linear polymer (C16H26O10N2) n that N-acetyl-D-glucose amine (GlcNAc) is combined into β-Isosorbide-5-Nitrae key.Extensively be present in the cell walls of Crustaceans shrimp, the crust of crab, the crust of insect, fungi (yeast, mould), algae and plant.Chitosan (Chitosan) is then the product that chitin sloughs C2 ethanoyl, has another name called chitosan, chitosan, soluble chitin, Chitosan etc., chemistry poly-(Isosorbide-5-Nitrae)-2-amino-2-deoxidation-B-D-glucose by name.
The production of current domestic chitin and chitosan mainly concentrates on coastal cities, is raw material greatly mainly with shrimp, crab shell, is that raw material obtains the very few of chitin and chitosan with fly maggot.Due to difference of originating, the acquisition of flyblow chitin and chitosan is discrepant with the acquisition of shrimp, crab shell allied substances in processing condition, and extracts flyblow chitin at present and chitosan method therefor is almost that overlapping in order to shrimp, crab shell is completely the extraction process of raw material.This method preparing chitin and chitosan with concentrated acid concentrated base, not only cost is high, energy consumption is large, product quality is poor, and can cause serious environmental pollution.
Flyblow chitin has very high pharmaceutical use, is also healthcare products important elements.Along with fly maggot industrialization cultivation is formed gradually, a lot of producer adds the making of fly maggot product.Wherein based on albumen and chitin.The preparation method of application number a kind of maggot compound protein powder that has been the patent disclosure of 201210084977.7, adopt enzymolysis process, cost is higher.And the method for your chitin do not extracted.
Summary of the invention
The present invention is that the further deep processing of residual residue is the chitin of food grade and medical grade after edible oil, protein powder to solve the comprehensive deep processing of fly maggot, improves the utilization ratio of fly maggot, provides a kind of preparation method of flyblow chitin.
The present invention is realized by following technical scheme: a kind of preparation method of flyblow chitin, comprises the steps:
(1) fly maggot is cleared up removal of impurities, microwave 10 ~ 15kg/KWh is dried, pulverizer adds fly maggot powder quality 3-5 normal butane doubly after pulverizing, in 30 ~ 36 DEG C of subcritical abstraction tanks, 1.5 ~ 1.8Bar extracts 20-40min, then filter, a filtrate 30-50 DEG C desolventizing obtains grease, and the normal temperature compressed post liquefaction recirculation of solvent n-butane uses, and filter residue is fly maggot spent meal;
(2) filter residue step (1) obtained and fly maggot spent meal add worm opaque amount 3-5 NaOH solution doubly, 10-60min is pulverized with colloidal mill, again with energy-collecting ultrasonic circulation all-in-one machine extraction 20 ~ 30min, then use horizontal screw centrifuge 3800 ~ 4000g centrifugal, residue is separated with protein solution, and residue is used for chitin and produces or preparation;
(3) by the residue that step (2) obtains, soak with the HCl of 10 ~ 30mmol/l, adopt energy-collecting ultrasonic circulation all-in-one machine decalcification 25 ~ 30min, then use horizontal screw centrifuge 3800 ~ 4000g centrifugal, filter residue is decalcification worm shell, i.e. chitin;
(4) spray-dried for the filter residue of step (3) gained device spraying dry is obtained chitin.
Present device is simple and easy, simple to operate, and preparation condition is easy to control, and is suitable for scale operation.
Embodiment
Embodiment 1: a kind of preparation method of flyblow chitin, comprises the steps:
(1) fly maggot is cleared up removal of impurities, microwave 10kg/KWh is dried, pulverizer adds the normal butane of fly maggot powder quality 3 times after pulverizing, in 30 DEG C of subcritical abstraction tanks, 1.5Bar extracts 20min, then filter, filtrate 30 DEG C of desolventizings obtain grease, and the normal temperature compressed post liquefaction recirculation of solvent n-butane uses, and filter residue is fly maggot spent meal;
(2) filter residue step (1) obtained and fly maggot spent meal add the NaOH solution of worm opaque amount 3 times, 10min is pulverized with colloidal mill, again with energy-collecting ultrasonic circulation all-in-one machine extraction 20min, then use horizontal screw centrifuge 3800g centrifugal, residue is separated with protein solution, and residue is used for chitin and produces or preparation;
(3) by the residue that step (2) obtains, soak with the HCl of 10mmol/L, adopt energy-collecting ultrasonic circulation all-in-one machine decalcification 25min, then use horizontal screw centrifuge 3800g centrifugal, filter residue is decalcification worm shell, i.e. chitin;
(4) the spray-dried device spraying dry of filter residue (feeding mouth temperature 165 DEG C, temperature out 80 DEG C) step (3) obtained obtains chitin.
Embodiment 2: a kind of preparation method of flyblow chitin, comprises the steps:
(1) fly maggot is cleared up removal of impurities, microwave 13kg/KWh is dried, pulverizer adds the normal butane of fly maggot powder quality 4 times after pulverizing, in 33 DEG C of subcritical abstraction tanks, 1.6Bar extracts 30min, then filter, filtrate 40 DEG C of desolventizings obtain grease, and the normal temperature compressed post liquefaction recirculation of solvent n-butane uses, and filter residue is fly maggot spent meal;
(2) filter residue step (1) obtained and fly maggot spent meal add the NaOH solution of worm opaque amount 4 times, 30min is pulverized with colloidal mill, again with energy-collecting ultrasonic circulation all-in-one machine extraction 25min, then use horizontal screw centrifuge 3900g centrifugal, residue is separated with protein solution, and residue is used for chitin and produces or preparation;
(3) by the residue that step (2) obtains, soak with the HCl of 20mmol/L, adopt energy-collecting ultrasonic circulation all-in-one machine decalcification 28min, then use horizontal screw centrifuge 3900g centrifugal, filter residue is decalcification worm shell, i.e. chitin;
(4) spray-dried for the filter residue of step (3) device spraying dry (feeding mouth temperature 170 DEG C, temperature out 85 DEG C) is obtained chitin.
Embodiment 3: a kind of preparation method of flyblow chitin, comprises the steps:
(1) fly maggot is cleared up removal of impurities, microwave 15kg/KWh is dried, pulverizer adds the normal butane of fly maggot powder quality 5 times after pulverizing, in 36 DEG C of subcritical abstraction tanks, 1.8Bar extracts 40min, then filter, filtrate 50 DEG C of desolventizings obtain grease, and the normal temperature compressed post liquefaction recirculation of solvent n-butane uses, and filter residue is fly maggot spent meal;
(2) filter residue step (1) obtained and fly maggot spent meal add the NaOH solution of worm opaque amount 5 times, 60min is pulverized with colloidal mill, again with energy-collecting ultrasonic circulation all-in-one machine extraction 30min, then use horizontal screw centrifuge 4000g centrifugal, residue is separated with protein solution, and residue is used for chitin and produces or preparation;
(3) by the residue that step (2) obtains, soak with the HCl of 30mmol/L, adopt energy-collecting ultrasonic circulation all-in-one machine decalcification 30min, then use horizontal screw centrifuge 4000g centrifugal, filter residue is decalcification worm shell, i.e. chitin;
(4) the spray-dried device spraying dry of filter residue (feeding mouth temperature 180 DEG C, temperature out 90 DEG C) step (3) obtained obtains chitin.
Embodiment 4: a kind of preparation method of flyblow chitin, comprises the steps:
(1) fly maggot is cleared up removal of impurities, microwave 15kg/KWh is dried, pulverizer adds the normal butane of fly maggot powder quality 3 times after pulverizing, in 34 DEG C of subcritical abstraction tanks, 1.7Bar extracts 30min, then filter, filtrate 50 DEG C of desolventizings obtain grease, and the normal temperature compressed post liquefaction recirculation of solvent n-butane uses, and filter residue is fly maggot spent meal;
(2) filter residue step (1) obtained and fly maggot spent meal add the NaOH solution of worm opaque amount 3 times, 40min is pulverized with colloidal mill, again with energy-collecting ultrasonic circulation all-in-one machine extraction 28min, then use horizontal screw centrifuge 4000g centrifugal, residue is separated with protein solution, and residue is used for chitin and produces or preparation;
(3) by the residue that step (2) obtains, soak with the HCl of 25mmol/L, adopt energy-collecting ultrasonic circulation all-in-one machine decalcification 25min, then use horizontal screw centrifuge 3800g centrifugal, filter residue is decalcification worm shell, i.e. chitin;
(4) the spray-dried device spraying dry of filter residue (feeding mouth temperature 180 DEG C, temperature out 88 DEG C) step (3) obtained obtains chitin.
The present invention adopts subcritical abstraction technology mass-producing degreasing to fly maggot meal first, reaches efficient, energy-conservation, zero release effect.Adopt energy-collecting ultrasonic cycling extraction technology, not only achieve the fairly large deproteinated of fly maggot spent meal, decalcification, reach efficient, energy-conservation, low emission effect simultaneously.Adopt sleeping spiral shell centrifugation technique, not only increase feed liquid deproteinated, depickling alkali efficiency, and improve the chitin rate of recovery.The present invention provide not only a kind of technical matters of easy large-scale production flyblow chitin, has also expanded fly maggot comprehensive exploitation simultaneously and has been worth.Present device is simple and easy, simple to operate, and preparation condition is easy to control, and is suitable for scale operation.
Claims (2)
1. a preparation method for flyblow chitin, is characterized in that: comprise the steps:
(1) fly maggot is cleared up removal of impurities, microwave 10 ~ 15kg/KWh is dried, pulverizer adds fly maggot powder quality 3-5 normal butane doubly after pulverizing, in 30 ~ 36 DEG C of subcritical abstraction tanks, 1.5 ~ 1.8Bar extracts 20-40min, then filter, a filtrate 30-50 DEG C desolventizing obtains grease, and the normal temperature compressed post liquefaction recirculation of solvent n-butane uses, and filter residue is fly maggot spent meal;
(2) filter residue step (1) obtained and fly maggot spent meal add worm opaque amount 3-5 NaOH solution doubly, 10-60min is pulverized with colloidal mill, again with energy-collecting ultrasonic circulation all-in-one machine extraction 20 ~ 30min, then use horizontal screw centrifuge 3800 ~ 4000g centrifugal, residue is separated with protein solution, and residue is used for chitin and produces or preparation;
(3) by the residue that step (2) obtains, soak with the HCl of 10 ~ 30mmol/l, adopt energy-collecting ultrasonic circulation all-in-one machine decalcification 25 ~ 30min, then use horizontal screw centrifuge 3800 ~ 4000g centrifugal, filter residue is decalcification worm shell, i.e. chitin;
(4) spray-dried for the filter residue of step (3) gained device spraying dry is obtained chitin.
2. the preparation method of a kind of flyblow chitin according to claim 1, is characterized in that: the dry feeding mouth temperature 165 ~ 180 DEG C of spray-dryer described in step (4), temperature out 80 ~ 90 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510904788.3A CN105384850A (en) | 2015-12-09 | 2015-12-09 | Preparation method of fly larva chitin |
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| CN201510904788.3A CN105384850A (en) | 2015-12-09 | 2015-12-09 | Preparation method of fly larva chitin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106939326A (en) * | 2017-04-18 | 2017-07-11 | 广东人为峰健康管理有限公司 | A kind of extraction separation method of fly-maggot protein and fly maggot oil |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011167185A (en) * | 2010-01-21 | 2011-09-01 | Institute Of National Colleges Of Technology Japan | Method for producing decomposed chitin |
| CN102199228A (en) * | 2010-03-30 | 2011-09-28 | 甘肃农业大学 | Preparation processes of flyblow chitin and chitosan and application of flyblow chitin in inducing trichoderma aureoviride T2 strains to produce chitinases |
| CN104878057A (en) * | 2015-04-22 | 2015-09-02 | 山西农业大学 | Large-scale production method for tenebrio molitor chitosan |
-
2015
- 2015-12-09 CN CN201510904788.3A patent/CN105384850A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011167185A (en) * | 2010-01-21 | 2011-09-01 | Institute Of National Colleges Of Technology Japan | Method for producing decomposed chitin |
| CN102199228A (en) * | 2010-03-30 | 2011-09-28 | 甘肃农业大学 | Preparation processes of flyblow chitin and chitosan and application of flyblow chitin in inducing trichoderma aureoviride T2 strains to produce chitinases |
| CN104878057A (en) * | 2015-04-22 | 2015-09-02 | 山西农业大学 | Large-scale production method for tenebrio molitor chitosan |
Non-Patent Citations (1)
| Title |
|---|
| 郎跃深主编: "《蝇蛆养殖关键技术与应用》", 30 May 2015, 科学技术文献出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106939326A (en) * | 2017-04-18 | 2017-07-11 | 广东人为峰健康管理有限公司 | A kind of extraction separation method of fly-maggot protein and fly maggot oil |
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