CN101787067B - Method for extraction of ergosterol by mushroom scrap - Google Patents
Method for extraction of ergosterol by mushroom scrap Download PDFInfo
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- CN101787067B CN101787067B CN2010101464062A CN201010146406A CN101787067B CN 101787067 B CN101787067 B CN 101787067B CN 2010101464062 A CN2010101464062 A CN 2010101464062A CN 201010146406 A CN201010146406 A CN 201010146406A CN 101787067 B CN101787067 B CN 101787067B
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Abstract
The invention discloses a method for the extraction of ergosterol by mushroom scrap, comprising a raw material pretreatment step and an ergosterol extraction step, wherein the raw material pretreatment step comprises: removing impurity, slicing, removing impurity once again, drying and crushing; the ergosterol extraction step sequentially comprises: saponification, filtration, addition of 70# solvent oil for extraction, washing of extraction liquid, evaporating concentration and refining ergosterol. According to the method, the finished product of ergosterol with higher accuracy is prepared by using waste mushroom scrap, which can not only reduce the environmental pollution caused by untidy pileup or burning of the mushroom scrap, but can also make full use of resources, save production cost and change waste into valuable resource.
Description
Technical field
The present invention relates to a kind of method of utilizing the mushroom tankage to extract ergosterol.
Background technology
Mushroom is natural food or multi-functional food, and not only delicious sweet aquatic foods, and contain abundant nutrition and the necessary mineral substance of human body also have medicinal efficacies such as relieving cough and reducing sputum, enhance immunity power simultaneously.Now, because the artificial growth of mushroom is more and more widely, the tube root excision that peasant household uses anorexia when plucking mushroom, thus produce a large amount of depleted mushroom tankage.Peasant household generally throws out without care when rubbish huddles when handling these mushroom tankage, and perhaps burning disposal had both produced greatly living environment and polluted, and caused the serious waste of resource again.
And ergosterol is a kind of important medicine chemical material, and it is used to prepare vitamin D2, " KE ", " hormone Progesterone " etc. in a large number.The ergosterol suitability for industrialized production mainly adopts fermentation method at present, and fermentation strain is mainly yeast, aspergillus tubigensis etc., collects yeast behind the yeast fermentation, obtains the ergosterol crystal through technologies such as saponification, extraction, crystallizations.It is long to produce ergosterol desired raw material incubation time through this method, and cost is high, and preparation technology's cycle is long, but gained ergosterol finished product purity is lower.
Summary of the invention
The present invention provides a kind of method of utilizing the mushroom tankage to extract ergosterol; Through this method the depleted tankage of pestering are prepared into the higher ergosterol finished product of precision; Can not only reduce because of the mushroom tankage huddle and put or burn the environmental pollution that is caused; But also can make full use of resource, and save production cost, realize turning waste into wealth.
The present invention adopts following technical scheme: a kind of method of utilizing the mushroom tankage to extract ergosterol, comprise the extraction step of feed pretreatment step and ergosterol, and said feed pretreatment step comprises:
A. removal of impurities: will pester the tankage screening to remove earth and impurity with linear vibrating screen;
B. section: the tankage of will pestering are cut into slices with slicing machine;
C. removal of impurities once more: put into the linear vibrating screen screening to the mushroom tankage section that above-mentioned steps b obtains with further removal impurity;
D. oven dry: put into the tunnel drying machine drying to the section of mushroom tankage and handle;
E. pulverize: the mushroom leftover bits and pieces tablet that will dry is pulverized and is granularity 40～60 purpose powder;
The extraction step of said ergosterol comprises:
A. saponification: the Powdered mushroom tankage and the alkali-pure mixed solution of above-mentioned raw materials pre-treatment step are mixed together; Under 75～85 ℃ temperature condition, stirred 2～3 hours and generate the saponification mixed solution; The quality proportioning of contained alkali, alcohol is 1: 0.2～0.4: 1.5～2.0 in said Powdered mushroom tankage and the mixed solution; Said alkali is sodium hydroxide or Pottasium Hydroxide, and said alcohol is methyl alcohol or ethanol;
B. filter: said saponification mixed solution is filtered, isolate the soluble impurity in the mixed solution, and obtain the saponification clear liquid.
C. adding No. 70 solvent oils extracts: add No. 70 solvent oil contents for 3 times in the saponification liquor that abovementioned steps B obtains; The volume ratio of each No. 70 solvent oils that add and saponification liquor is 1: 1; 50～55 ℃ of extraction temperature are isolated the extraction liquid that contains ergosterol then;
D. washing of extraction liquid: the extraction liquid that contains ergosterol with 50 ℃ deionized water wash more than 3 times;
E. evaporation concentration: the extraction liquid heating after will washing makes its progressively concentrate, dry, obtain the ergosterol bullion;
F. ergosterol is refining: putting into concentration to the ergosterol bullion in the above-mentioned steps is that 95% ethanolic soln makes its dissolving, in solution, adds gac, and is heated to temperature and is 65～70 ℃ and decolours; Ethanol ergosterol after the decolouring processing is slowly cooled to 5～10 ℃, left standstill crystallization 24～48 hours; Suction filtration goes out crystallisate, uses the washing with alcohol crystallisate, promptly obtains the finished product ergosterol after the vacuum-drying.
Soluble impurity during said step B filtered is poured the ethanol liquid after washing in the said saponification liquor into 95% washing with alcohol 3 times again.
The present invention extracts ergosterol through utilizing the mushroom tankage, and it has following beneficial effect:
1, the present invention is raw materials for production with depleted mushroom tankage, and this feed distribution is extensive, and the source is abundant.
2, technological process of the present invention is simple, and process cycle is shorter, and prepared ergosterol finished product purity is high.
It is raw material that the present invention adopts depleted mushroom tankage; Be prepared into the high ergosterol finished product of purity through simple processing sequence; Not only can reduce because of the mushroom tankage huddle and put or burn the environmental pollution that is caused, and can also make full use of resource, save to produce and drop into and production cost; For society creates considerable economic, realize turning waste into wealth.
Embodiment
Embodiment 1:
Present embodiment utilization mushroom tankage extract the method for ergosterol, comprise the extraction step of feed pretreatment step and ergosterol, and wherein, feed pretreatment step comprises: will pester the tankage screening to remove earth and impurity with linear vibrating screen; The tankage of will pestering are cut into slices with slicing machine; Put into the linear vibrating screen screening to the section of mushroom tankage with further removal impurity; The tankage of will pestering section is put into the tunnel drying machine drying and is handled, and pulverizing then is that granularity 40～60 purposes are Powdered;
The extraction step of ergosterol comprises successively:
(1) saponification: the Powdered mushroom tankage and the alkali-pure mixed solution of above-mentioned raw materials pre-treatment step are mixed together, under 80 ℃ temperature condition, stirred 3 hours and generate the saponification mixed solution, wherein, employed alkali is sodium hydroxide, and alcohol is methyl alcohol; The quality proportioning of contained sodium hydroxide, methyl alcohol is 1: 0.2: 1.5 in Powdered mushroom tankage and the mixed solution;
(2) filter: the saponification mixed solution is filtered, isolate the soluble impurity in the mixed solution, and obtain the saponification clear liquid; And it is 95% washing with alcohol 3 times that soluble impurity is used concentration, pours the ethanol liquid after the washing in the above-mentioned saponification liquor into again;
(3) adding No. 70 solvent oils extracts: add No. 70 solvent oil contents for 3 times in the saponification liquor that abovementioned steps (2) obtain; The volume ratio of each No. 70 solvent oils that add and saponification liquor is 1: 1; 55 ℃ of extraction temperature are isolated the extraction liquid that contains ergosterol then;
(4) washing of extraction liquid: the extraction liquid that contains ergosterol with 50 ℃ deionized water wash 3 times;
(5) evaporation concentration: the extraction liquid heating after will washing makes it progressively concentrated, dry, obtains the ergosterol bullion;
(6) ergosterol is refining: putting into concentration to the ergosterol bullion in the above-mentioned steps is that 95% ethanolic soln makes its dissolving, in solution, adds gac, and is heated to temperature and is 70 ℃ and decolours; Ethanol ergosterol after the decolouring processing is slowly cooled to 10 ℃, left standstill crystallization 48 hours; Suction filtration goes out crystallisate, uses the washing with alcohol crystallisate, and crystallisate is carried out promptly obtaining the finished product ergosterol after the vacuum-drying.
Alkali in said step (1) saponification can be that sodium hydroxide, Pottasium Hydroxide or the two mix, and alcohol can be methyl alcohol, ethanol or both mixing.
Embodiment 2:
The feed pretreatment step of present embodiment is identical with embodiment 1;
The extraction step of ergosterol comprises successively:
(1) saponification: the Powdered mushroom tankage and the alkali-pure mixed solution of above-mentioned raw materials pre-treatment step are mixed together, under 85 ℃ temperature condition, stirred 2 hours and generate the saponification mixed solution, wherein, employed alkali is Pottasium Hydroxide, and alcohol is ethanol; Contained Pottasium Hydroxide, alcoholic acid quality proportioning are 1: 0.3: 2.0 in Powdered mushroom tankage and the mixed solution;
(2) filter: the saponification mixed solution is filtered, isolate the soluble impurity in the mixed solution, and obtain saponification liquor free from foreign meter; And it is 95% washing with alcohol 3 times that soluble impurity is used concentration, pours the ethanol liquid after the washing in the above-mentioned saponification liquor into again.
(3) adding No. 70 solvent oils extracts: add No. 70 solvent oil contents for 3 times in the saponification liquor that abovementioned steps (2) obtain; The volume ratio of each No. 70 solvent oils that add and saponification liquor is 1: 1; 50 ℃ of extraction temperature are isolated the extraction liquid that contains ergosterol then;
(4) washing of extraction liquid: the extraction liquid that contains ergosterol with 50 ℃ deionized water wash 5 times;
(5) evaporation concentration: the extraction liquid heating after will washing progressively concentrates until drying it, obtains the ergosterol bullion;
(6) ergosterol is refining: putting into concentration to the ergosterol bullion in the above-mentioned steps is that 95% ethanolic soln makes its dissolving, in solution, adds gac, and is heated to temperature and is 65 ℃ and decolours; Ethanol ergosterol after the decolouring processing is slowly cooled to 5 ℃, left standstill crystallization 24 hours; Suction filtration goes out crystallisate, uses the washing with alcohol crystallisate, and crystallisate is carried out promptly obtaining the finished product ergosterol after the vacuum-drying.
Embodiment 3:
The feed pretreatment step of present embodiment is identical with embodiment 1;
The extraction step of ergosterol comprises successively:
(1) saponification: the Powdered mushroom tankage and the alkali-pure mixed solution of above-mentioned raw materials pre-treatment step are mixed together; Under 80 ℃ temperature condition, stirred 2.5 hours and generate the saponification mixed solution; Wherein, Employed alkali is sodium hydroxide, Pottasium Hydroxide mixture, and alcohol is methyl alcohol, alcoholic acid mixture; Contained Pottasium Hydroxide, alcoholic acid quality proportioning are 1: 0.2: 1.8 in Powdered mushroom tankage and the mixed solution;
(2) filter: the saponification mixed solution is filtered, isolate the soluble impurity in the mixed solution, and obtain the saponification clear liquid; And it is 95% washing with alcohol 3 times that soluble impurity is used concentration, pours the ethanol liquid after the washing in the above-mentioned saponification liquor into again.
(3) adding No. 70 solvent oils extracts: add No. 70 solvent oil contents for 3 times in the saponification liquor that abovementioned steps (2) obtain; The volume ratio of each No. 70 solvent oils that add and saponification liquor is 1: 1; 53 ℃ of extraction temperature are isolated the extraction liquid that contains ergosterol then;
(4) washing of extraction liquid: the extraction liquid that contains ergosterol use concentration be 8% acetic acid soln washing once, use 50 ℃ deionized water wash 4 times then;
(5) evaporation concentration: the extraction liquid heating after will washing progressively concentrates until drying it, obtains the ergosterol bullion;
(6) ergosterol is refining: putting into concentration to the ergosterol bullion in the above-mentioned steps is that 95% ethanolic soln makes its dissolving, in solution, adds gac, and is heated to temperature and is 70 ℃ and decolours; Ethanol ergosterol solution after the decolouring processing is slowly cooled to 5 ℃, left standstill crystallization 48 hours; Suction filtration goes out crystallisate, uses the washing with alcohol crystallisate, and crystallisate is carried out promptly obtaining the finished product ergosterol after the vacuum-drying.
Above-mentionedly be merely embodiment of the present invention, but design concept of the present invention is not limited thereto, allly utilizes this design that the present invention is carried out the change of unsubstantiality, all should belong to the behavior of invading protection domain of the present invention.
Claims (2)
1. method of utilizing the mushroom tankage to extract ergosterol comprises the extraction step of feed pretreatment step and ergosterol, and said feed pretreatment step comprises:
A. removal of impurities: will pester the tankage screening to remove earth and impurity with linear vibrating screen;
B. section: the tankage of will pestering are cut into slices with slicing machine;
C. removal of impurities once more: put into the linear vibrating screen screening to the mushroom tankage section that above-mentioned steps b obtains with further removal impurity;
D. oven dry: put into the tunnel drying machine drying to the section of mushroom tankage and handle;
E. pulverize: the mushroom leftover bits and pieces tablet that will dry is pulverized and is granularity 40～60 purpose powder;
The extraction step of said ergosterol comprises successively:
A. saponification: the Powdered mushroom tankage and the alkali-pure mixed solution of above-mentioned raw materials pre-treatment step are mixed together; Under 75～85 ℃ temperature condition, stirred 2～3 hours and generate the saponification mixed solution; The quality proportioning of contained alkali, alcohol is 1: 0.2～0.3: 1.5～2.0 in said Powdered mushroom tankage and the mixed solution; Said alkali is sodium hydroxide or Pottasium Hydroxide, and said alcohol is methyl alcohol or ethanol;
B. filter: said saponification mixed solution is filtered, isolate the soluble impurity in the mixed solution, and obtain the saponification clear liquid;
C. adding No. 70 solvent oils extracts: add No. 70 solvent oil contents for 3 times in the saponification liquor that abovementioned steps B obtains; The volume ratio of each No. 70 solvent oils that add and saponification liquor is 1: 1; 50～55 ℃ of extraction temperature are isolated the extraction liquid that contains ergosterol then;
D. washing of extraction liquid: the extraction liquid that contains ergosterol with 50 ℃ deionized water wash more than 3 times;
E. evaporation concentration: the extraction liquid heating after will washing progressively concentrates until drying it, obtains the ergosterol bullion;
F. ergosterol is refining: putting into concentration to the ergosterol bullion in the above-mentioned steps is that 95% ethanolic soln makes its dissolving, in solution, adds gac, and is heated to temperature and is 65～70 ℃ and decolours; Ethanol ergosterol after the decolouring processing is slowly cooled to 5～10 ℃, left standstill crystallization 24～48 hours; Suction filtration goes out crystallisate, uses the washing with alcohol crystallisate, promptly obtains the finished product ergosterol after the vacuum-drying.
2. a kind of method of utilizing the mushroom tankage to extract ergosterol according to claim 1; It is characterized in that: it is 95% washing with alcohol 3 times that the soluble impurity during said step B is filtered uses concentration, pours the ethanol liquid after the washing in the said saponification liquor into again.
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| CN2010101464062A CN101787067B (en) | 2010-04-14 | 2010-04-14 | Method for extraction of ergosterol by mushroom scrap |
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| CN2010101464062A CN101787067B (en) | 2010-04-14 | 2010-04-14 | Method for extraction of ergosterol by mushroom scrap |
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| CN101787067A CN101787067A (en) | 2010-07-28 |
| CN101787067B true CN101787067B (en) | 2012-07-04 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102659890B (en) * | 2012-04-12 | 2014-07-02 | 中南林业科技大学 | Woody ergosterol preparation method |
| CN110156654B (en) * | 2018-03-23 | 2020-12-22 | 浙江慧和健康科技有限公司 | High-purity edible fungus vitamin D2Preparation process of extract |
| CN108558977A (en) * | 2018-05-08 | 2018-09-21 | 刘健 | A method of extracting ergosterol from amylofermentation object |
| CN109280070A (en) * | 2018-05-21 | 2019-01-29 | 陕西蓝米信息技术有限公司 | A kind of extracting method of ergosterol |
| CN110669092B (en) * | 2019-10-25 | 2022-04-05 | 宿迁医美科技有限公司 | Method for extracting ergosterol from vinasse |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322728A (en) * | 2000-05-09 | 2001-11-21 | 北京化工大学 | Method of extracting ergosterol from waste mycelium |
| CN1733930A (en) * | 2005-08-29 | 2006-02-15 | 勐永药业公司 | Ergosterol preparation method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322728A (en) * | 2000-05-09 | 2001-11-21 | 北京化工大学 | Method of extracting ergosterol from waste mycelium |
| CN1733930A (en) * | 2005-08-29 | 2006-02-15 | 勐永药业公司 | Ergosterol preparation method |
Non-Patent Citations (3)
| Title |
|---|
| Pirjo Mattila et al.Sterol and vitamin D2 contents in some wild and cultivated mushrooms.《Food Chemistry》.2002,第76卷293-298. * |
| 白晨等.香菇中麦角甾醇生成维生素D2的光化反应实验设计.《实验室研究与探索》.2010,第29卷(第2期),16-19. * |
| 高虹等.利用微波辅助提取测定姬松茸中麦角甾醇含量.《浙江大学学报(农业与生命科学版)》.2007,第33卷(第1期),113-118. * |
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