CN105360566A - Compound protein source as well as preparation and application thereof - Google Patents
Compound protein source as well as preparation and application thereof Download PDFInfo
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- CN105360566A CN105360566A CN201510902850.5A CN201510902850A CN105360566A CN 105360566 A CN105360566 A CN 105360566A CN 201510902850 A CN201510902850 A CN 201510902850A CN 105360566 A CN105360566 A CN 105360566A
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- protein source
- compound protein
- protease
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/06—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from blood
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Microbiology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to a compound protein source as well as preparation and application thereof, and belongs to the fields of nutrients and feeds of aquatic livestock. The compound protein source is characterized by consisting of the following components in percentage by weight: 5-6% of porcine blood hydrolysate, 5-6% of soybean meal hydrolysate, 10-15% of fish meal, 25-30% of soybean meal, 20-25% of corn protein powder, 5-18% of vital wheat gluten, and 10-15% of peanut meal. Compared with a commercial feed, a feed added with the compound protein source disclosed by the invention has the advantages that the growing performance of bass can be notably improved, the feed factor is reduced, the non-specific immunological competence of organisms is improved, and the consumption of fish meal in the bass feed is reduced.
Description
Technical field
The invention belongs to aquatic livestock nutrition and feed field, relate to a kind of preparation and application thereof of compound protein source particularly.
Background technology
The most pressing issues that the sharp increase of population brings provide food for the mankind, and aquatic products are as a class high protein, low-fat high value food in food, and people increase severely to its demand nature and day.Protein is the topmost nutriment of aquatic animal feed, is also the key affecting feed cost.Aquatic livestock especially marine fishery animal requires high to protein content in feed, strong to animal protein source dependence, causes the cost of aquiculture animal feed generally higher (Gatlin etc., 2007).Fish meal is the main protein sources of predacious fish feed, is also the main component forming feed cost, and because the increase of fish meal demand and supply keep relative stability, thus fish meal was maintaining very high price in recent years always.These plant protein sources all forcing people to have to as far as possible find on the one hand cheapness carry out Peru Fish Dietary.A kind of effective measures solving current fish meal shortage problem with cheap plant protein source Substitute For Partial fish meal, but due to plant protein source contain the defect such as ANFs, amino acid imbalance limit its addition in aquatic feeds (Zhou Qi deposits, 2005; Bureauetal, 1998; Francisetal, 2001; Gomesetal, 1995; Chen Wei etc., 2009; Deyabetal, 2003; Village equality, 2002), up to the present, when substitute with vegetable protein major part or the feed all after fish meal feed predacious fish time, make feed meet the known nutritional need of fish even if add exogenous nutrition material, but still there will be growth significantly reduction.Therefore, development of new protein sources Peru Fish Dietary is the task of top priority.
Perch (Lateolabraxjaponicus) claims flower perch, stockaded village spends, accompanies perch etc., belongs to Perciformes, Sushi section, and perch belongs to flower, and current breeding seawater fish crop is according to domestic first place.At present the research work with animal and plant-derived albumen Peru Fish Dietary has been carried out to perch, mainly concentrate on the interpolation of the removal of ANFs, crystal amino acid and various plants albumen used in combination.But still there is following problem:
(1) Peru Fish Dietary protein content is limited;
(2) application technology imperfection, does not form scale product to the application of predacious fish.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of compound protein source and preparation and application thereof, the present invention can solve the problem that a large amount of use plant protein source causes the minimizing of fish rate, poor growth and autoimmunity to decline, and makes up the deficiencies in the prior art.
A kind of compound protein source, is characterized in that it is made up of pig blood hydrolysate 5-6%, dregs of beans hydrolysate 5-6%, fish meal 10-15%, dregs of beans 25-30%, corn protein powder 20-25%, Gluten 5-18% and peanut meal 10-15%; Each composition is weight ratio above, and described pig blood hydrolysate carries out enzymolysis acquisition by Alcalase alkali protease and Flavourzyme flavor protease two kinds of enzyme Swine blood meal; Described dregs of beans protolysate to be hydrolyzed to dregs of beans by cellulase, Alcalase alkali protease and Flavourzyme flavor protease three kinds of protease to obtain.
Further, the preparation method of described pig blood hydrolysate, Swine blood meal is after 1:7 adds water by weight proportion, add Alcalase alkali protease and Flavourzyme flavor protease two kinds of enzymes again, the consumption of enzyme is adjusted to Alcalase1-1.5ml/100g material, and Flavourzyme1-1.5g/100g expects, regulate pH to 8.5-9.0,55 DEG C of hydrolysis 4-5 hour, heat up and make enzyme deactivation, water-bath 20-30min; Cooling, filters, then at room temperature centrifugally obtains coarse filtration enzymolysis liquid, concentrated enzymolysis liquid, then spraying dry.
Further, the preparation method of described dregs of beans protolysate, adopt 3 kinds of protease substeps to dregs of beans hydrolysis, 3 kinds of described protease are cellulase, Alcalase alkali protease and Flavourzyme flavor protease; First carry out enzymolysis with cellulase to dregs of beans, the enzymatic hydrolysis condition of cellulase: cellulase 3g/100g, material water weight ratio 1:7, regulates pH to 4.8-5.2, and 45 DEG C are hydrolyzed 4 ~ 5 hours; Then Alcalase alkali protease and Flavourzyme flavor protease two kinds of enzymes are added, the consumption of enzyme is adjusted to Alcalase1-1.5ml/100g, Flavourzyme1-1.5g/100g, the part by weight 1:7 of material water, regulate pH to 8.5-9.0,55 DEG C are hydrolyzed 4 ~ 5 hours; Be warming up to and make enzyme deactivation, water-bath 20 ~ 30min; Cooling, filters, then at room temperature centrifugally obtains coarse filtration enzymolysis liquid, concentrated enzymolysis liquid, then spraying dry.
The preparation method in above-mentioned compound protein source, is characterized in that first 7 described components being pulverized respectively, crosses 80-120 eye mesh screen; Then weigh respectively; Fully mix, be compound protein source of the present invention.
Above-mentioned compound protein source is as the application of perch feed.
Perch feed containing described compound protein source
Above-mentioned high efficiency composition protein sources is as the application of fish meal
Fish meal containing described compound protein source
Aquatic feeds containing described compound protein source
High efficiency composition albumen of the present invention adds 80% (percetage by weight, lower same) in perch feed, without the need to adding fish meal protein again; The growth performance of perch can be improved compared with commercial feed, improve immunity, not affect perch survival, growth, fish body biochemical composition.
Detailed description of the invention
Embodiment
Below by embodiment, technical scheme of the present invention is further explained, but protection scope of the present invention is not by any pro forma restriction of embodiment.
High efficiency composition protein sources of the present invention is made up of pig blood hydrolysate 5-6%, dregs of beans hydrolysate 5-6%, fish meal 10-15%, dregs of beans 25-30%, corn protein powder 20-25%, Gluten 5-18%, peanut meal 10-15%.
The preparation of above-mentioned pig blood hydrolysate, Swine blood meal is after 1:7 adds water by weight proportion, add Alcalase alkali protease and Flavourzyme flavor protease two kinds of enzymes again, the consumption of enzyme can be adjusted to Alcalase1-1.5ml/100g, Flavourzyme1-1.5g/100g, the part by weight 1:7 of material water, regulates pH to 8.5-9.0, and 55 DEG C are hydrolyzed 4.5 hours.Be warming up to 95 DEG C and make enzyme deactivation, water-bath 25min.Cooling, filter, then at room temperature the centrifugal 8min of 4500r/min obtains coarse filtration enzymolysis liquid, uses Rotary Evaporators to concentrate and obtains concentrate, then spraying dry.
The preparation of above-mentioned dregs of beans protolysate, adopts three kinds of protease substeps to be hydrolyzed to dregs of beans: cellulase, Alcalase alkali protease and Flavourzyme flavor protease, obtain enzymolysis liquid.The enzymatic hydrolysis condition of cellulase: cellulase 3g/100g, material water weight ratio 1:7, regulates pH to 4.8-5.2, and 45 DEG C are hydrolyzed 4.5 hours.Then Alcalase alkali protease and Flavourzyme flavor protease two kinds of enzymes are added, the consumption of enzyme heightens Alcalase1-1.5ml/100g, Flavourzyme1-1.5g/100g, the part by weight 1:7 of material water, regulate pH to 8.5-9.0,55 DEG C are hydrolyzed 4.5 hours.Be warming up to 95 DEG C and make enzyme deactivation, water-bath 20 ~ 30min.Cooling, filter, then at room temperature the centrifugal 8min of 4500r/min obtains coarse filtration enzymolysis liquid, uses Rotary Evaporators to concentrate enzymolysis liquid, obtains concentrate, then spraying dry.
7 components are first pulverized by the preparation method of above-mentioned high efficiency composition albumen respectively, cross 80-120 mesh sieve; Then weigh respectively; Finally in mixer, (according to the order that weight is ascending) adds above-mentioned weighted each component step by step, mixes (requiring the coefficient of variation≤5%), is compound plant protein of the present invention.
Above-mentioned compound plant protein is as the application of perch feed.
In the existing perch feed of every 100kg, adding the above-mentioned compound protein prepared of 80kg, without the need to adding fish meal again, adding flour 10kg, fish oil 5.5kg, choline 1kg, calcium monohydrogen phosphate 1.5kg, vitamin mixtures 1kg, mineral mixture 1kg, mixes, and makes feed.
For verifying effect of the present invention, adopting feed A and commercial feed B of the present invention, carrying out contrast test, two kinds of feeds are expanded pellet diet.Commercial feed B fish meal content is 30%, crude protein 42%, fat 12%.Experiment is carried out in Ningbo City, Zhejiang Province Xiangshan Gangwan Aquatic Product Offspring Seed Co., Ltd. seawater floating cage.Choose 6 net cages (3.0m × 3.0m × 3.0m), often kind of feed establish 3 parallel, choose health, perch that specification is consistent puts into net cage, 200 tails thrown in by every net cage, and the culture-cycle is 2 months.Initial mass is (31.05 ± 1.54) g, cultivation terminates rear mensuration growth performance and immune indexes as table 1, table 2, experimental result shows, growth performance and protein efficiency ratio (P<0.05) that above-mentioned compound protein source group significantly improves perch is added in feed, significantly reduce feed coefficient, liver body ratio (P<0.05), to coefficient of condition and dirty body than not having a significant impact (P>0.05).From table 2, add above-mentioned compound protein source group perch liver activate the phagocytic capacity, complement activity, lysozyme activity, superoxide dismutase activity, activity of acid phosphatase, alkaline phosphatase activities and TAC and significantly improve (P<0.05).
Table 1 protein sources is on the impact of perch growth performance
| Index | Commercial feed B | Invention feed A |
| Rate of body weight gain (%) | 228.96±33.81 a | 266.24±17.56 b |
| Specific growth rate (%/d) | 2.28±0.12 a | 2.72±0.11 b |
| Feed coefficient | 1.14±0.07 b | 0.84±0.06 a |
| Protein efficiency ratio | 2.12±0.13 a | 2.89±0.16 b |
| Liver body ratio | 1.82±0.17 b | 1.29±0.35 a |
| Dirty body ratio | 9.95±0.37 | 9.02±0.51 |
| Coefficient of condition (g/cm3) | 1.44±0.05 | 1.46±0.04 |
Table 2 protein sources is on the impact of perch Liver immunity index
Protein sources is compared with commercial feed in sum, can significantly improve the growth performance of perch, reduces feed coefficient, improves body nospecific immunity active, reduces the consumption of fish meal.
According to those skilled in the art's general knowledge, described compound protein source can be carried out interpolation as protein sources equally and be used in other fish or aquatic feeds.Its addition those skilled in the art can obtain according to general knowledge or contrast test, and can produce a desired effect.
Claims (9)
1. a compound protein source, is characterized in that it is made up of pig blood hydrolysate 5-6%, dregs of beans hydrolysate 5-6%, fish meal 10-15%, dregs of beans 25-30%, corn protein powder 20-25%, Gluten 5-18% and peanut meal 10-15%; Each composition is weight ratio above, and described pig blood hydrolysate carries out enzymolysis acquisition by Alcalase alkali protease and Flavourzyme flavor protease two kinds of enzyme Swine blood meal; Described dregs of beans protolysate to be hydrolyzed to dregs of beans by cellulase, Alcalase alkali protease and Flavourzyme flavor protease three kinds of protease to obtain.
2. a kind of compound protein source according to claim 1, it is characterized in that the preparation method of described pig blood hydrolysate, Swine blood meal is after 1:7 adds water by weight proportion, add Alcalase alkali protease and Flavourzyme flavor protease two kinds of enzymes again, the consumption of enzyme is adjusted to Alcalase1-1.5ml/100g material, and Flavourzyme1-1.5g/100g expects, regulate pH to 8.5-9.0,55 DEG C of hydrolysis 4-5 hour, heat up and make enzyme deactivation, water-bath 20-30min; Cooling, filters, then at room temperature centrifugally obtains coarse filtration enzymolysis liquid, concentrated enzymolysis liquid, then spraying dry.
3. a kind of compound protein source according to claim 1, it is characterized in that the preparation method of described dregs of beans protolysate, adopt 3 kinds of protease substeps to dregs of beans hydrolysis, 3 kinds of described protease are cellulase, Alcalase alkali protease and Flavourzyme flavor protease; First carry out enzymolysis with cellulase to dregs of beans, the enzymatic hydrolysis condition of cellulase: cellulase 3g/100g, material water weight ratio 1:7, regulates pH to 4.8-5.2, and 45 DEG C are hydrolyzed 4 ~ 5 hours; Then Alcalase alkali protease and Flavourzyme flavor protease two kinds of enzymes are added, the consumption of enzyme is adjusted to Alcalase1-1.5ml/100g, Flavourzyme1-1.5g/100g, the part by weight 1:7 of material water, regulate pH to 8.5-9.0,55 DEG C are hydrolyzed 4 ~ 5 hours; Be warming up to and make enzyme deactivation, water-bath 20 ~ 30min; Cooling, filters, then at room temperature centrifugally obtains coarse filtration enzymolysis liquid, concentrated enzymolysis liquid, then spraying dry.
4. a kind of compound protein source according to claim 1, is characterized in that the preparation method in compound protein source, first 7 described components is pulverized respectively, crosses 80-120 eye mesh screen; Then weigh respectively; Fully mix, be compound protein source of the present invention.
5. according to the application of the compound protein source of claim 1-4 described in any one as perch feed.
6. the perch feed containing claim 1-4 compound protein source described in any one.
7. the compound protein source of claim 1-4 described in any one is as the application of fish meal.
8. the fish meal containing claim 1-4 compound protein source described in any one.
9. the aquatic feeds containing claim 1-4 compound protein source described in any one.
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| CN201510902850.5A CN105360566A (en) | 2015-12-09 | 2015-12-09 | Compound protein source as well as preparation and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105941863A (en) * | 2016-05-18 | 2016-09-21 | 广东海洋大学 | Low-fish-meal compound protein source and application thereof to preparation of grouper feed |
| CN107319194A (en) * | 2017-06-30 | 2017-11-07 | 佛山市信豚生物科技有限公司 | A kind of compound protein source and preparation method and application |
| CN108419909A (en) * | 2018-03-13 | 2018-08-21 | 西南大学 | A kind of mulberry leaf compound protein source and preparation method thereof substituting fish meal |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530162A (en) * | 2008-03-10 | 2009-09-16 | 北京大北农科技集团股份有限公司 | Preparation method of novel protein feed additive |
| CN101773194A (en) * | 2010-01-15 | 2010-07-14 | 北京华达杰瑞生物技术有限公司 | Method for extracting soy peptide from soybean meals |
| CN103478434A (en) * | 2013-10-10 | 2014-01-01 | 中国水产科学研究院黄海水产研究所 | Novel composite protein source and its application in turbot feed |
| CN104171260A (en) * | 2013-07-31 | 2014-12-03 | 北京澳龙港生物技术研究中心 | Functional peptide protein powder, and preparation method and applications thereof |
-
2015
- 2015-12-09 CN CN201510902850.5A patent/CN105360566A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530162A (en) * | 2008-03-10 | 2009-09-16 | 北京大北农科技集团股份有限公司 | Preparation method of novel protein feed additive |
| CN101773194A (en) * | 2010-01-15 | 2010-07-14 | 北京华达杰瑞生物技术有限公司 | Method for extracting soy peptide from soybean meals |
| CN104171260A (en) * | 2013-07-31 | 2014-12-03 | 北京澳龙港生物技术研究中心 | Functional peptide protein powder, and preparation method and applications thereof |
| CN103478434A (en) * | 2013-10-10 | 2014-01-01 | 中国水产科学研究院黄海水产研究所 | Novel composite protein source and its application in turbot feed |
Non-Patent Citations (2)
| Title |
|---|
| 曹如燕: "猪血酶解物抗氧化活性的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 闫桂云: "水产饲料中鱼粉替代物的研究进展", 《北京水产》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105941863A (en) * | 2016-05-18 | 2016-09-21 | 广东海洋大学 | Low-fish-meal compound protein source and application thereof to preparation of grouper feed |
| CN105941863B (en) * | 2016-05-18 | 2020-05-29 | 广东海洋大学 | Low-fish-meal composite protein source and application thereof in preparation of grouper feed |
| CN107319194A (en) * | 2017-06-30 | 2017-11-07 | 佛山市信豚生物科技有限公司 | A kind of compound protein source and preparation method and application |
| CN108419909A (en) * | 2018-03-13 | 2018-08-21 | 西南大学 | A kind of mulberry leaf compound protein source and preparation method thereof substituting fish meal |
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Application publication date: 20160302 |