CN105349598A - Method for modifying starch through enzymatic degradation technology - Google Patents
Method for modifying starch through enzymatic degradation technology Download PDFInfo
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- CN105349598A CN105349598A CN201510913458.0A CN201510913458A CN105349598A CN 105349598 A CN105349598 A CN 105349598A CN 201510913458 A CN201510913458 A CN 201510913458A CN 105349598 A CN105349598 A CN 105349598A
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- starch
- process modification
- enzyme liberating
- rhizoma dioscoreae
- glucoamylase
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Abstract
The invention discloses a method for modifying starch through the enzymatic degradation technology. Chinese yam starch is used as a raw material. After natural starch is gelatinized, the starch is modified through the enzymatic degradation technology on the condition of auxiliaries such as a lauryl sodium sulfate solution, then, dicarboxylic acid derivative glutaric acid is added, the stability of the modified starch is improved, and finally acetone cleaning, washing and vacuum drying are performed. The technology is short in process and easy to implement and has the wide application prospect.
Description
Technical field
The present invention relates to a kind of starch conversion method, particularly one utilizes Rhizoma Dioscoreae starch, and the method for the sex change prepared by enzyme liberating technique belongs to starch Chemical Industry field.
Background technology
Starch is the storage characteristics carbohydrate that in cereal kernel, proportion is maximum, is a kind of polysaccharide, and molecular formula is (C
6h
10o
5)
n.Starch is that its molecular structure a kind of can be degraded, and have the starting material of thermoplastic properties, but because poorly water-soluble, Application in Chemical Engineering is very limited, therefore adopts treated starch often.Native starch, through suitable chemical treatment, is introduced some chemical group and is made molecular structure or its physico-chemical property is changed, thus generates modified starch.
Porous-starch is the one of modified starch, and porous produces very large specific surface area, and thus porous-starch is mainly used as the carrier of absorption.Porous-starch, compared with other sorbent materials, except having good absorption property, also has the characteristics such as biodegradable, production technique simple, be widely used.Preparing in porous-starch process, not only its hole count, aperture, hole depth can be controlled, also modification easily can be carried out according to adsorbed material characteristic to it.Such as, when adsorbed material is apolar substance, non-polar group can be connected on the surface of porous-starch, thus strengthen the specificity of its absorption.Therefore can be used as a kind of efficient, nontoxic, safe sorbent material, be widely used in the industries such as food, medical and health, agricultural, papermaking, printing, makeup, washing composition, tackiness agent.
At present, modification is carried out to native starch and prepares porous-starch and mainly contain following several method: one is physical method, such as, by ultrasonic irradiation or spray art; Two is adopt mechanical means, such as, by mechanical impacts such as ball-milling technologies; 3rd is biochemical method, such as alcohol sex change or acid hydrolysis, and in above-mentioned several method, the production cost of the mechanical impact such as ultrasonic irradiation, ball-milling technology method is higher, not easily realizes industrial sector; And spray method and alcohol sex change are formed is a kind of solid popcorn polymer spheroid, adsorption only occurs in the rough gully of spherome surface, and adsorptive capacity is more limited, and application prospect is pessimistic; Acid-hydrolysis method speed of reaction under gelatinization point is comparatively slow, and degraded differs, and randomness is strong, is not easily formed poroid, limits the application of acid system.
Chinese yam stem tuber contains much starch, except moisture accounts for most of ratio, starch accounts for 20%, the present invention, using Rhizoma Dioscoreae starch as synthesis material, adopts enzyme liberating technique, controls spherex particle diameter and microvoid structure by regulating preparation process condition and adding suitable adjuvants, thus modification is carried out to Rhizoma Dioscoreae starch, the modified porous starch specific surface area of preparation is large, has stronger adsorption, have wide application prospect to many kinds of substance.
Summary of the invention
The object of this invention is to provide a kind of method of Rhizoma Dioscoreae starch being carried out to modification, its main method steps is as follows:
(1) first mixed with distilled water by a certain amount of Rhizoma Dioscoreae starch, gelatinization is carried out in heating;
(2) be cooled to room temperature after gelatinization, then add appropriate sodium dodecyl sulfate solution, be uniformly dissolved;
(3) again solution is heated, then add a certain amount of glucoamylase, stir and make abundant reaction;
(4) add a certain amount of pentanedioic acid, fully react;
(5), more successively with acetone cleaning, washing, filter;
(6) last vacuum-drying, obtains a kind of Chinese yam treated starch.
Preferentially, step (1) in, Heating temperature is 60-70 DEG C.
Preferentially, step (2) in, the amount of the sodium lauryl sulphate of interpolation and the ratio of Rhizoma Dioscoreae starch are 1mL:5g.
Preferentially, step (3) in, system Heating temperature is 40-50 DEG C.
Preferentially, step (3) in, the glucoamylase of interpolation comes from aspergillus niger, and the mass ratio of glucoamylase and Rhizoma Dioscoreae starch is 1:50.
Preferentially, step (4) in, the amount of the pentanedioic acid of interpolation and the ratio of Rhizoma Dioscoreae starch are 1mL:100g.
Preferentially, step (6) in, vacuum-drying temperature is no more than 70 DEG C.
The present invention has following advantages and characteristic:
(1) the modified starch good stability prepared;
(2) modified technique is simple, easily operates.
Embodiment
Embodiment one:
Get a beaker and load 5g Rhizoma Dioscoreae starch, pour 100ml distilled water again into, be heated to 60 DEG C and carry out gelatinization, room temperature is cooled to after abundant gelatinization, add 1mL sodium lauryl sulphate, and stirring makes it be uniformly dissolved, then when solution being heated to 40 DEG C, then the glucoamylase of 0.1g is added, and stirring makes abundant reaction, then add the pentanedioic acid of 0.05g, stir after making it fully, then pour in acetone, precipitation washes with water, after cleaning, takes out precipitation, be positioned over vacuum-drying in 70 DEG C of environment, after drying, namely obtain a kind of Chinese yam treated starch.
Embodiment two:
Get a beaker and load 10g Rhizoma Dioscoreae starch, pour 200ml distilled water again into, be heated to 65 DEG C and carry out gelatinization, room temperature is cooled to after abundant gelatinization, add 2mL sodium lauryl sulphate, and stirring makes it be uniformly dissolved, then when solution being heated to 45 DEG C, then the glucoamylase of 0.2g is added, and stirring makes abundant reaction, then add the pentanedioic acid of 0.1g, stir after making it fully, then pour in acetone, precipitation washes with water, after cleaning, takes out precipitation, be positioned over vacuum-drying in 65 DEG C of environment, after drying, namely obtain a kind of Chinese yam treated starch.
Embodiment three:
Get a beaker and load 20g Rhizoma Dioscoreae starch, pour 300ml distilled water again into, be heated to 70 DEG C and carry out gelatinization, room temperature is cooled to after abundant gelatinization, add 4mL sodium lauryl sulphate, and stirring makes it be uniformly dissolved, then when solution being heated to 50 DEG C, then the glucoamylase of 0.1g is added, and stirring makes abundant reaction, then add the pentanedioic acid of 0.2g, stir after making it fully, then pour in acetone, precipitation washes with water, after cleaning, takes out precipitation, be positioned over vacuum-drying in 60 DEG C of environment, after drying, namely obtain a kind of Chinese yam treated starch.
Claims (7)
1. an enzyme liberating process modification starch method, it is characterized in that, the method comprises the steps:
(1) first mixed with distilled water by a certain amount of Rhizoma Dioscoreae starch, gelatinization is carried out in heating;
(2) be cooled to room temperature after gelatinization, then add appropriate sodium dodecyl sulfate solution, be uniformly dissolved;
(3) again solution is heated, then add a certain amount of glucoamylase, stir and make abundant reaction;
(4) add a certain amount of pentanedioic acid, fully react;
(5), more successively with acetone cleaning, washing, filter;
(6) last vacuum-drying, obtains a kind of Chinese yam treated starch.
2. a kind of enzyme liberating process modification starch method according to claim 1, is characterized in that, step (1) in, Heating temperature is 60-70 DEG C.
3. a kind of enzyme liberating process modification starch method according to claim 1, is characterized in that, step (2) in, the amount of the sodium lauryl sulphate of interpolation and the ratio of Rhizoma Dioscoreae starch are 1mL:5g.
4. a kind of enzyme liberating process modification starch method according to claim 1, is characterized in that, step (3) in, system Heating temperature is 40-50 DEG C.
5. a kind of enzyme liberating process modification starch method according to claim 1, is characterized in that, step (3) in, the glucoamylase of interpolation comes from aspergillus niger, and the mass ratio of glucoamylase and Rhizoma Dioscoreae starch is 1:50.
6. a kind of enzyme liberating process modification starch method according to claim 1, is characterized in that, step (4) in, the amount of the pentanedioic acid of interpolation and the ratio of Rhizoma Dioscoreae starch are 1mL:100g.
7. a kind of enzyme liberating process modification starch method according to claim 1, is characterized in that, step (6) in, drying temperature is no more than 70 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510913458.0A CN105349598A (en) | 2015-12-12 | 2015-12-12 | Method for modifying starch through enzymatic degradation technology |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510913458.0A CN105349598A (en) | 2015-12-12 | 2015-12-12 | Method for modifying starch through enzymatic degradation technology |
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| CN105349598A true CN105349598A (en) | 2016-02-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510913458.0A Pending CN105349598A (en) | 2015-12-12 | 2015-12-12 | Method for modifying starch through enzymatic degradation technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106316158A (en) * | 2016-08-31 | 2017-01-11 | 郭舒洋 | Method for preparing impregnating compound from compound modified tapioca starch |
| CN117064996A (en) * | 2023-10-08 | 2023-11-17 | 江苏鸿祺生物科技有限公司 | Composition for protecting prostate and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1173995C (en) * | 2002-07-11 | 2004-11-03 | 华南理工大学 | Enzymic starch catabolin and its preparing process |
| CN1661028A (en) * | 2004-12-22 | 2005-08-31 | 华南理工大学 | Method for preparing porous starch through enzyme method in high temperature |
-
2015
- 2015-12-12 CN CN201510913458.0A patent/CN105349598A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1173995C (en) * | 2002-07-11 | 2004-11-03 | 华南理工大学 | Enzymic starch catabolin and its preparing process |
| CN1661028A (en) * | 2004-12-22 | 2005-08-31 | 华南理工大学 | Method for preparing porous starch through enzyme method in high temperature |
Non-Patent Citations (1)
| Title |
|---|
| 林江涛等: "微孔性变性淀粉的研究", 《郑州粮食学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106316158A (en) * | 2016-08-31 | 2017-01-11 | 郭舒洋 | Method for preparing impregnating compound from compound modified tapioca starch |
| CN117064996A (en) * | 2023-10-08 | 2023-11-17 | 江苏鸿祺生物科技有限公司 | Composition for protecting prostate and preparation method thereof |
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Application publication date: 20160224 |
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