CN107345234A - A kind of method of enzyme degradation technique modified starch - Google Patents
A kind of method of enzyme degradation technique modified starch Download PDFInfo
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- CN107345234A CN107345234A CN201710181516.4A CN201710181516A CN107345234A CN 107345234 A CN107345234 A CN 107345234A CN 201710181516 A CN201710181516 A CN 201710181516A CN 107345234 A CN107345234 A CN 107345234A
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- starch
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/22—Preparation of compounds containing saccharide radicals produced by the action of a beta-amylase, e.g. maltose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
Abstract
The invention discloses a kind of method of enzyme degradation technique modified starch, comprise the following steps that：Starch is tuned into starch milk with water, then it is alkalescence to adjust starch milk with sodium hydroxide solution；Sodium hypochlorite, stirring reaction are added, the pH value for controlling starch milk is 89；After being cooled to room temperature, sodium dodecyl sulfate solution is added, is uniformly dissolved；Add vinylacetate, stirring reaction；Polyvinyl alcohol is added in gained starch milk, continues to react；600 1000U/g amylase is added, after 4 8h are handled at 45 55 DEG C, washed reaction liquid, solid content is collected and is dried in vacuo to obtain modified starch.Leptosomatic starch molecule is transformed into the even closer starch branched structure of structure of pyknic using Q-enzyrne by the present invention, so as to reduce the speed of amylopectin retrogradation, reach the purpose for suppressing starch retrogradation, the starch of production has the features such as hydrophobicity, good fluidity, can be widely used in household chemicals.
Description
Technical field
The present invention relates to a kind of starch conversion method, specifically a kind of method of enzyme degradation technique modified starch.
Background technology
Starch is the storage characteristics carbohydrate that proportion is maximum in cereal kernel, is a kind of polysaccharide, and starch is a kind of
Its molecular structure can degrade, and have the raw material of thermoplastic properties, but because poorly water-soluble, Application in Chemical Engineering is by the very day of one's doom
System, therefore often use modified starch.Native starch by appropriate chemical treatment, introduce some chemical groups make molecular structure or
Its physicochemical property is set to change, so as to generate converted starch.
Porous-starch is one kind of converted starch, porous to produce very big specific surface area, thus porous-starch is mainly used as
The carrier of absorption.Porous-starch is compared with other adsorbents, in addition to good absorption property, also with it is biodegradable,
Simple production process, the characteristic such as it is widely used.During porous-starch is prepared, its hole count, aperture, hole depth are not only can control,
Also it can be easily modified according to adsorbed material characteristic., can be for example, when adsorbed material is apolar substance
The surface of porous-starch connects non-polar group, so as to strengthen the selectivity of its absorption.Therefore can be used as it is a kind of it is efficient, nontoxic,
The adsorbent of safety, is widely used in food, medical and health, agricultural, papermaking, printing, cosmetics, detergent, adhesive
Etc. industry.
At present, native starch is modified and preparing porous-starch mainly has following several method：When physical method,
Such as pass through ultrasonic irradiation or spray art；Second, using mechanical means, such as pass through the mechanical impacts such as ball-milling technology；The
Third, biochemical method, such as alcohol denaturation or sour water solution, in above-mentioned several method, the machinery such as ultrasonic irradiation, ball-milling technology
The production cost of shock method is higher, is not easy to realize industrial industrialization；And spray-on process is denatured that to be formed is a kind of solid with alcohol
Popcorn polymer spheroid, suction-operated only occurred in the rough gully of spherome surface, and adsorbance is than relatively limited, application prospect
It is pessimistic；Acid-hydrolysis method reaction rate under gelatinization point is slower, and degraded differs, and randomness is strong, is not easy to be formed poroid, limits
The application of acid system.
The content of the invention
It is an object of the invention to provide a kind of method of simple, the maneuverable enzyme degradation technique modified starch of technique,
To solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of method of enzyme degradation technique modified starch, is comprised the following steps that：
(1) starch is tuned into mass fraction 10-30% starch milk with water, takes 50-70 part starch milks, then use sodium hydroxide
Solution regulation starch milk is alkalescence；
(2) sodium hypochlorite is added, stirring reaction 3-5h at being 30-40 DEG C in temperature, the pH value for controlling starch milk is 8-9；
(3) after being cooled to room temperature, 10-14 part sodium dodecyl sulfate solutions is added, are uniformly dissolved；
(4) it is 20-40% by gained starch milk washs and adjusts starch milk mass concentration in step (3), adds 20-40 parts
Vinylacetate, stirring reaction 2-4h at being 25-45 DEG C in temperature；
(5) polyvinyl alcohol of 10-30 parts is added into starch milk obtained by step (4), continues to react 30-50min；
(6) 600-1000U/g amylase is added, after handling 4-8h at 45-55 DEG C, washed reaction liquid, collects solid content
It is dried in vacuo to obtain modified starch.
As the further scheme of the present invention：The amylase is α-maltogenic amylase, alpha-amylase or beta amylase.
As the further scheme of the present invention：The starch be rice starch, farina, tapioca, wheaten starch,
One or more of mixing in rice starch, bean starch.
As the further scheme of the present invention：The concentration of sodium hydroxide solution is 0.8-1mol/L in the step (1).
As further scheme of the invention：Washing is washed successively with ethanol and distilled water in the step (4).
Compared with prior art, the beneficial effects of the invention are as follows：
Leptosomatic starch molecule is transformed into the even closer shallow lake of structure of pyknic using Q-enzyrne by the present invention
Powder branched structure, so as to reduce the speed of amylopectin retrogradation, reach the purpose for suppressing starch retrogradation, the starch of production has
The features such as hydrophobicity, good fluidity, can be widely used in household chemicals.
Embodiment
The technical scheme of this patent is described in more detail with reference to embodiment.
Embodiment 1
A kind of method of enzyme degradation technique modified starch, is comprised the following steps that：
(1) starch is tuned into the starch milk of mass fraction 10% with water, takes 50 parts of starch milks, then with concentration is 0.8mo1/L
Sodium hydroxide solution regulation starch milk for alkalescence；
(2) sodium hypochlorite is added, stirring reaction 3h at being 30 DEG C in temperature, the pH value for controlling starch milk is 8；
(3) after being cooled to room temperature, 10 parts of sodium dodecyl sulfate solutions is added, are uniformly dissolved；
(4) it is 20% to wash and adjust starch milk mass concentration gained starch milk in step (3), adds 20 parts of acetic acid second
Alkene ester, stirring reaction 2h at being 25 DEG C in temperature；
(5) 10 parts of polyvinyl alcohol is added into starch milk obtained by step (4), continues to react 30min；
(6) 600U/g amylase is added, after handling 4h at 45 DEG C, washed reaction liquid, solid content progress vacuum is collected and does
It is dry to obtain modified starch.
Embodiment 2
A kind of method of enzyme degradation technique modified starch, is comprised the following steps that：
(1) starch is tuned into the starch milk of mass fraction 15% with water, takes 55 parts of starch milks, then with concentration is 0.85mol/
L sodium hydroxide solution regulation starch milk is alkalescence；
(2) sodium hypochlorite is added, stirring reaction 3.5h at being 32 DEG C in temperature, the pH value for controlling starch milk is 8.2；
(3) after being cooled to room temperature, 11 parts of sodium dodecyl sulfate solutions is added, are uniformly dissolved；
(4) it is 25% to wash and adjust starch milk mass concentration gained starch milk in step (3), adds 25 parts of acetic acid second
Alkene ester, stirring reaction 2.5h at being 30 DEG C in temperature；
(5) 15 parts of polyvinyl alcohol is added into starch milk obtained by step (4), continues to react 35min；
(6) 700U/g amylase is added, after handling 5h at 48 DEG C, washed reaction liquid, solid content progress vacuum is collected and does
It is dry to obtain modified starch.
Embodiment 3
A kind of method of enzyme degradation technique modified starch, is comprised the following steps that：
(1) starch is tuned into the starch milk of mass fraction 20% with water, takes 60 parts of starch milks, then with concentration is 0.9mol/L
Sodium hydroxide solution regulation starch milk for alkalescence；
(2) sodium hypochlorite is added, stirring reaction 4h at being 35 DEG C in temperature, the pH value for controlling starch milk is 8.5；
(3) after being cooled to room temperature, 12 parts of sodium dodecyl sulfate solutions is added, are uniformly dissolved；
(4) it is 30% to wash and adjust starch milk mass concentration gained starch milk in step (3), adds 30 parts of acetic acid second
Alkene ester, stirring reaction 3h at being 35 DEG C in temperature；
(5) 20 parts of polyvinyl alcohol is added into starch milk obtained by step (4), continues to react 40min；
(6) 800U/g amylase is added, after handling 6h at 50 DEG C, washed reaction liquid, solid content progress vacuum is collected and does
It is dry to obtain modified starch.
Embodiment 4
A kind of method of enzyme degradation technique modified starch, is comprised the following steps that：
(1) starch is tuned into the starch milk of mass fraction 25% with water, takes 65 parts of starch milks, then with concentration is 0.95mol/
L sodium hydroxide solution regulation starch milk is alkalescence；
(2) sodium hypochlorite is added, stirring reaction 4.5h at being 38 DEG C in temperature, the pH value for controlling starch milk is 8.8；
(3) after being cooled to room temperature, 13 parts of sodium dodecyl sulfate solutions is added, are uniformly dissolved；
(4) it is 35% to wash and adjust starch milk mass concentration gained starch milk in step (3), adds 35 parts of acetic acid second
Alkene ester, stirring reaction 3.5h at being 40 DEG C in temperature；
(5) 25 parts of polyvinyl alcohol is added into starch milk obtained by step (4), continues to react 45min；
(6) 900U/g amylase is added, after handling 7h at 52 DEG C, washed reaction liquid, solid content progress vacuum is collected and does
It is dry to obtain modified starch.
Embodiment 5
A kind of method of enzyme degradation technique modified starch, is comprised the following steps that：
(1) starch is tuned into the starch milk of mass fraction 30% with water, takes 70 parts of starch milks, then with concentration is 1mol/L's
Sodium hydroxide solution regulation starch milk is alkalescence；
(2) sodium hypochlorite is added, stirring reaction 5h at being 40 DEG C in temperature, the pH value for controlling starch milk is 9；
(3) after being cooled to room temperature, 14 parts of sodium dodecyl sulfate solutions is added, are uniformly dissolved；
(4) it is 40% to wash and adjust starch milk mass concentration gained starch milk in step (3), adds 40 parts of acetic acid second
Alkene ester, stirring reaction 4h at being 45 DEG C in temperature；
(5) 30 parts of polyvinyl alcohol is added into starch milk obtained by step (4), continues to react 50min；
(6) 1000U/g amylase is added, after handling 8h at 55 DEG C, washed reaction liquid, solid content progress vacuum is collected and does
It is dry to obtain modified starch.
The amylase is α-maltogenic amylase, alpha-amylase or beta amylase；The starch is rice starch, potato
Any or several mixing in starch, tapioca, wheaten starch, rice starch, bean starch.
Leptosomatic starch molecule is transformed into the even closer shallow lake of structure of pyknic using Q-enzyrne by the present invention
Powder branched structure, so as to reduce the speed of amylopectin retrogradation, reach the purpose for suppressing starch retrogradation, the starch of production has
The features such as hydrophobicity, good fluidity, can be widely used in household chemicals.
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
Formula, can also be on the premise of this patent objective not be departed from one skilled in the relevant art's possessed knowledge
Various changes can be made.
Claims (5)
- A kind of 1. method of enzyme degradation technique modified starch, it is characterised in that comprise the following steps that：(1) starch is tuned into mass fraction 10-30% starch milk with water, takes 50-70 part starch milks, then use sodium hydroxide solution Starch milk is adjusted as alkalescence；(2) sodium hypochlorite is added, stirring reaction 3-5h at being 30-40 DEG C in temperature, the pH value for controlling starch milk is 8-9；(3) after being cooled to room temperature, 10-14 part sodium dodecyl sulfate solutions is added, are uniformly dissolved；(4) it is 20-40% by gained starch milk washs and adjusts starch milk mass concentration in step (3), adds 20-40 part acetic acid Vinyl acetate, stirring reaction 2-4h at being 25-45 DEG C in temperature；(5) polyvinyl alcohol of 10-30 parts is added into starch milk obtained by step (4), continues to react 30-50min；(6) 600-1000U/g amylase is added, after handling 4-8h at 45-55 DEG C, washed reaction liquid, solid content is collected and carries out Vacuum drying obtains modified starch.
- 2. the method for enzyme degradation technique modified starch according to claim 1, it is characterised in that the amylase is α-wheat Bud saccharogenic amylase, alpha-amylase or beta amylase.
- 3. the method for enzyme degradation technique modified starch according to claim 1, it is characterised in that the starch forms sediment for rice One or more of mixing in powder, farina, tapioca, wheaten starch, rice starch, bean starch.
- 4. the method for enzyme degradation technique modified starch according to claim 1, it is characterised in that hydrogen in the step (1) The concentration of sodium hydroxide solution is 0.8-1mol/L.
- 5. the method for enzyme degradation technique modified starch according to claim 1, it is characterised in that washed in the step (4) Wash is washed successively with ethanol and distilled water.
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Cited By (6)
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| CN108300745A (en) * | 2017-12-29 | 2018-07-20 | 齐鲁工业大学 | A kind of method that complex enzyme prepares starch special |
| CN108396041A (en) * | 2018-03-16 | 2018-08-14 | 江南大学 | A method of improving starch liquefacation product transparency |
| CN110846360A (en) * | 2019-11-29 | 2020-02-28 | 江南大学 | Method for inhibiting starch retrogradation |
| CN111484392A (en) * | 2020-05-06 | 2020-08-04 | 厦门阿胜生态科技有限公司 | Preparation method of solid linalool |
| CN112779124A (en) * | 2021-01-06 | 2021-05-11 | 抚州市鹤达实业有限公司 | Modified starch production device using enzyme |
| CN115058466A (en) * | 2022-07-29 | 2022-09-16 | 江南大学 | Freeze-thaw stable type biological modified starch and preparation method thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300745A (en) * | 2017-12-29 | 2018-07-20 | 齐鲁工业大学 | A kind of method that complex enzyme prepares starch special |
| CN108300745B (en) * | 2017-12-29 | 2021-04-27 | 齐鲁工业大学 | Method for preparing special modified starch by using complex enzyme |
| CN108396041A (en) * | 2018-03-16 | 2018-08-14 | 江南大学 | A method of improving starch liquefacation product transparency |
| WO2019174137A1 (en) * | 2018-03-16 | 2019-09-19 | 江南大学 | Method for improving transparency of starch liquefied product |
| US11124816B2 (en) | 2018-03-16 | 2021-09-21 | Jiangnan University | Method for improving the transparency of starch liquefaction |
| CN110846360A (en) * | 2019-11-29 | 2020-02-28 | 江南大学 | Method for inhibiting starch retrogradation |
| CN110846360B (en) * | 2019-11-29 | 2022-03-15 | 江南大学 | Method for inhibiting starch retrogradation |
| CN111484392A (en) * | 2020-05-06 | 2020-08-04 | 厦门阿胜生态科技有限公司 | Preparation method of solid linalool |
| CN112779124A (en) * | 2021-01-06 | 2021-05-11 | 抚州市鹤达实业有限公司 | Modified starch production device using enzyme |
| CN115058466A (en) * | 2022-07-29 | 2022-09-16 | 江南大学 | Freeze-thaw stable type biological modified starch and preparation method thereof |
| CN115058466B (en) * | 2022-07-29 | 2023-08-25 | 江南大学 | Freeze-thawing stable biological modified starch and preparation method thereof |
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Address after: 277400 Residence of Malantun Town, Taierzhuang District, Zaozhuang City, Shandong Province Applicant after: Shandong Shenzhou Xiangyu Science and Technology Group Co., Ltd. Address before: 277400 Residence of Malantun Town, Taierzhuang District, Zaozhuang City, Shandong Province Applicant before: Shandong Shenzhou Xiangyu Technology Group Co., Ltd. |
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Application publication date: 20171114 |