CN105342917B - whitening cream based on natural extract and preparation method thereof - Google Patents
whitening cream based on natural extract and preparation method thereof Download PDFInfo
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- CN105342917B CN105342917B CN201510816168.4A CN201510816168A CN105342917B CN 105342917 B CN105342917 B CN 105342917B CN 201510816168 A CN201510816168 A CN 201510816168A CN 105342917 B CN105342917 B CN 105342917B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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Abstract
The present invention provides a kind of preparation method of the whitening cream based on natural extract, it includes the following steps：The number that following steps occur is weight ratio, S1, the purple grape for taking 50 80 parts, 50 100 parts of blueberry, 40 60 parts of mulberries, it is placed in container, and add 100 160 parts of water, mashing is stirred by mixer and forms pulp mixed liquor within 30 60 minutes, by temperature control at 46 DEG C, rotating speed is 400 450 revs/min；S2, the pulp mixed liquor to purple grape, blueberry and mulberries carry out traditional vacuum and handle to obtain slurries, for mixing pulp mixed liquor again and removing the pulp slag of purple grape, blueberry and mulberries；Add 34 parts of cellulases into slurries to be digested, at 22 25 DEG C, slurries digest 5 10 days the temperature of enzymolysis, are less than 3% to remaining monose, separation tank switching obtains zymotic fluid；S3, by zymotic fluid stand 47 it is small when, temperature control is at 46 DEG C；Take the supernatant liquor of the zymotic fluid after standing；Supernatant liquor is transferred to container for evaporation and carries out heating evaporation, and other steps.
Description
Technical field
The present invention relates to cosmetic skincare product preparing technical field, especially a kind of whitening cream based on natural extract
And preparation method thereof.
Background technology
With the development of society and the improvement of people's living standards, requirement of the consumer to daily nursing product be also increasingly
Height, requires the effect of to product also increasingly to refine.Because yellow's colour of skin problem, Chinese's generally existing colour of skin are obscure, dim
The problems such as yellow, shortage gloss, easy allergy, pigment deposition.Expert data shows that pigmentation phenomenon accounts for about 80% in women
Ratio, and another item data show Chinese women 80% be all Combination skin, the irregular colour of skin is that this kind of skin faces most
Big problem, how whitening, and one of most concerned topic of Chinese Consumer's.
But or existing whitening product is largely formulated by chemicals, the facial skin of user is stimulated compared with
Greatly.Formed in whitening product using various natural plants (mainly similar to the plant of fruits and vegetables) proportioning.
Such as the Chinese patent literature of Publication No. CN104825655A, disclose a kind of nti-freckle, whitening, moulding combination
Thing, includes following components：100-150 parts of rosebud powder, 105-155 parts of gelatin powder, 200-300 parts of matrimony vine, dried rhizome of rehmannia 70-
130 parts, 100-150 parts of linseed, 20-41 parts, Icing Sugar 80-120 of soybean, 3-75 parts of oligomeric maltose, 8-12 parts of sodium citrate,
8-12 parts of magnesium stearate, 100-124 parts of dextrin.People are difficult to know which component is effective to whitening by said ratio, which
A little components do not work whitening.
The Chinese patent literature of Publication No. CN104825379A for another example, discloses a kind of U.S. for reducing melanin deposition
Shining skin late frost in vain, the weight proportion of each bulk pharmaceutical chemicals is as follows, is parts by weight：Lycopene 20-30, cow-bezoar 5-15, aloe 5-15,
It is kelp 6-16, cucumber 10-20, chamomile 15-25 green-tea extracts 6-10, Caulis et Folium Lactucae sativae extract 8-20, vitamin e1 0-15, sweet
Oily 2-6, astaxanthin 5-9.The patent there is also it is above-mentioned same the defects of.The ox even mentioned in the patent document specification
The effect of Huang, kelp, does not just contact at all with whitening effect at all.
What although the skin whitener therefore formed in the prior art using natural plants proportioning was formulated relative to chemicals
Skin whitener safety, but since the component played a role in various fruits and vegetables to whitening is unknown and mutual between various composition
Mechanism of action is not known, therefore the whitening effect of the skin whitener formed using various fruits and vegetables proportioning is matched somebody with somebody with respect to chemicals
It is poor to make the skin whitener formed.
The content of the invention
In view of this, the present invention provides a kind of both harmless, and cosmetic result is good based on natural extract
Whitening cream and preparation method thereof.
The present invention also provides a kind of preparation method of the whitening cream based on natural extract, it includes the following steps：With
Under the number that occurs of each step be weight ratio,
S1, the purple grape for taking 50-80 parts, 50-100 parts of blueberry, 40-60 parts of mulberries, are placed in container, and add
100-160 parts of water, is stirred mashing by mixer and forms pulp mixed liquor in 30-60 minutes, by temperature control in 4-6
DEG C, rotating speed is 400-450 revs/min；
S2, the pulp mixed liquor to purple grape, blueberry and mulberries carry out traditional vacuum and handle to obtain slurries；Add into slurries
Enter 3-4 parts of cellulases to be digested, at 22-25 DEG C, slurries digest 5-10 days the temperature of enzymolysis, to remaining monose are less than
3%, separation tank switching obtains zymotic fluid；
S3, by zymotic fluid stand 4-7 it is small when, temperature control is at 4-6 DEG C；Take the supernatant liquor of the zymotic fluid after standing；Will
Supernatant liquor is transferred to container for evaporation and carries out heating evaporation, by the pressure control in container for evaporation in 1800 to 2600 pas, until
Water content drops to 10-15% the first pastes of formation in supernatant liquor in container for evaporation；
S4, by the first paste take out be put into it is spare in storage container, by the temperature control in storage container in 12-18
DEG C, the oxygen content in storage container air is controlled 2% to 3%；
S5, the patter of rain haematococcus for taking 30-60 parts, and patter of rain haematococcus is placed into 3-5 at 26-28 DEG C of shady and cool ventilation
My god, 40-50 parts of reticulate millettia is taken, haematococcus pluvialis and reticulate millettia mixed grinding are formed into the mixed powder that particle diameter is 0.05-0.1mm
End；The water that 80-100 parts are added in mixed-powder is dissolved, and the filter for being 0.30-0.35 μm with aperture after traditional vacuum processing
Film carries out that patter of rain haematococcus and the hybrid filtering liquid of reticulate millettia is obtained by filtration；
S6, adjust the pH value of patter of rain haematococcus and the hybrid filtering liquid of reticulate millettia to using hole again after 7.15-7.25
Footpath is that 0.30-0.35 μm of filter membrane carries out that cleaner liquid was obtained by filtration, and heating evaporation is carried out to crossing cleaner liquid by container for evaporation,
By the pressure control in container for evaporation in 1000 to 1400 pas, until the water content in cleaner liquid of crossing in container for evaporation drops to
20-35% forms the second paste；
S7, take 30-35 parts of dried rainworms and 2-3 portions of Schisandra chinensis to be ground, and forms the mixing that particle diameter is 0.05-0.1mm and does
Powder powder；30-50 parts of ether dissolution will be added in mixed powder powder, remove undissolved impurity and obtain, and ether is steamed
Send out the solid matter after being evaporated；
The first paste, the second paste and step of step S6 of S8, mixed merga pass blender whipping step S3
Solid matter in S7, and 15-18 parts of emulsifying agent is added, whitening cream finished product is formed when stirring 2-4 is small, the speed of stirring is
700-800 revs/min, by the temperature control in blender at 12-18 DEG C.
In the preparation method of the whitening cream of the present invention based on natural extract, also wrapped after step S8
Include：
Whitening cream finished product, be positioned in sealing container by S9, and the condition in sealing container is as follows：Oxygen content in air
Control is passed through helium and is maintained at a standard atmospheric pressure 2% to 3%, and atmospheric pressure error is empty within positive and negative 5 percent
For gas relative humidity in 40%-45%, temperature scope is 12-18 DEG C；
S10, by ultraviolet sterilize sealing container 60-120 minute, and the intensity of ultraviolet disinfection is every square centimeter
100-110 microwatts.
In the preparation method of the whitening cream of the present invention for being based on natural extract,
Trans 2.5-2.6 parts of dioxy morin, 4-8 parts of motherwort powder, sub- oil are additionally added when being stirred in the step S8
It is 1.3-1.7 parts sour.
The present invention is formulated for the skin whitener formed in the prior art using natural plants proportioning relative to chemicals
Skin whitener safety, but since the component played a role in various fruits and vegetables to whitening is unknown, and the phase between various composition
Interaction mechanism is not known, therefore the whitening effect of the skin whitener formed using various fruits and vegetables proportioning is with respect to chemicals
The defects of skin whitener being formulated is poor, extracts component beneficial to whitening in various natural materials, and is determined by experiment
The ratio of the beneficial component of each group of whitening.
Brief description of the drawings
Fig. 1 is the flow chart of the preparation method of the whitening cream based on natural extract.
Embodiment
The principle of the present invention is explained below by way of various embodiments of the invention.
As described in Figure 1, the embodiment of the present invention also provides a kind of preparation method of the whitening cream based on natural extract, its
Include the following steps：The number that following steps occur is weight ratio,
S1, the purple grape for taking 50-80 parts, 50-100 parts of blueberry, 40-60 parts of mulberries, are placed in container, and add
100-160 parts of water, is stirred mashing by mixer and forms pulp mixed liquor in 30-60 minutes, by temperature control in 4-6
DEG C, rotating speed is 400-450 revs/min.By temperature control at 4-6 DEG C, the delicate flavour of purple grape, blueberry, mulberries can be carried out most
The preservation of big degree.Meanwhile by the time control of mashing at 30-60 minutes, and rotating speed control turns in 400-450, first, energy
Enough to realize sufficiently grinding, two are that of avoiding the various nutritional ingredients in the above-mentioned fruit of heat destruction that excessive rotating speed is brought
Activity.Rotating speed control is turned in 400-450, the effect of grinding can be admirably taken into account and avoid generating heat.Rich in super in purple grape
Superoxide dismutase, blueberry is rich in anthocyanidin, and resveratrol oligomer class is higher compared to other fruit in mulberries.
S2, the pulp mixed liquor to purple grape, blueberry and mulberries carry out traditional vacuum and handle to obtain slurries；Add into slurries
Enter 3-4 parts of cellulases to be digested, the temperature of enzymolysis is fermented 5-10 days after 22-25 DEG C, slurries enzymolysis, to remaining monose
Less than 3%, separation tank switching obtains zymotic fluid.By carrying out traditional vacuum processing to the pulp mixed liquor of purple grape, blueberry and mulberries,
Purple grape and blueberry can more fully be mixed.Can be monose by the polysaccharide conversion in slurries by cellulase,
At the same time by fermentation, the monose in the zymotic fluid for being can be less than 3% (weight ratio), specifically, can be surveyed by gas-chromatography
Determine the contents of monosaccharides in method measure zymotic fluid.Reduce interference of the sugar to whitening composition.
S3, by zymotic fluid stand 4-7 it is small when, temperature control is at 4-6 DEG C；Take the supernatant liquor of the zymotic fluid after standing；Will
Supernatant liquor is transferred to container for evaporation and carries out heating evaporation, by the pressure control in container for evaporation in 1800 to 2600 pas, until
Water content drops to 10-15% the first pastes of formation in supernatant liquor in container for evaporation., can by temperature control at 4-6 DEG C
To slow down the oxidation of zymotic fluid, and the pressure control in container for evaporation can be caused less than 10 in 1800 to 2600 pas
Under conditions of DEG C, zymotic fluid rapidly evaporates.The standard of supernatant liquor is the impurity that is invisible to the naked eye.
S4, by the first paste take out be put into it is spare in storage container, by the temperature control in storage container in 12-18
DEG C, the oxygen content in storage container air is controlled 2% to 3%.This step can greatly delay the oxidation of paste.
S5, the patter of rain haematococcus for taking 30-60 parts, and patter of rain haematococcus is placed into 3-5 at 26-28 DEG C of shady and cool ventilation
My god, 40-50 parts of reticulate millettia is taken, haematococcus pluvialis and reticulate millettia mixed grinding are formed into the mixed powder that particle diameter is 0.05-0.1mm
End；The water that 80-100 parts are added in mixed-powder is dissolved, and the filter for being 0.30-0.35 μm with aperture after traditional vacuum processing
Film carries out that patter of rain haematococcus and the hybrid filtering liquid of reticulate millettia is obtained by filtration.Astaxanthin, astaxanthin generation are rich in patter of rain haematococcus
One of most strong natural in boundary, the oxygen radical in effective scavenger-cell, strengthens cytothesis ability, maintains body
Balance and the accumulation for reducing senile cell, protect cell and DNA health from inside to outside, so that skin health is protected, rather than only
Only it is the effect for playing whitening.It is rich in formoononetin in reticulate millettia, the methoxy isoflavone in formoononetin enables to
DOPA has been converted back into tyrosine in melanocyte, so as to suppress the generation of melanin.
S6, adjust the pH value of patter of rain haematococcus and the hybrid filtering liquid of reticulate millettia to using hole again after 7.15-7.25
Footpath is that 0.30-0.35 μm of filter membrane carries out that cleaner liquid was obtained by filtration, and can filter out impurity.By container for evaporation to crossing filtering
Liquid carries out heating evaporation, and by the pressure control in container for evaporation in 1000 to 1400 pas, cleaner liquid is crossed under this air pressure can be with
Evaporated rapidly under conditions of less than 10 DEG C, avoid damage to the active ingredient of whitening, until crossing in cleaner liquid in container for evaporation
Water content drops to 20-35% and forms the second paste, avoids that water content is too low to cause the first paste and the second paste
Bad mixing, be also unlikely to water content it is excessive caused by finished product concentration it is low the defects of.
S7, take 30-35 parts of dried rainworms and 2-3 portions of Schisandra chinensis to be ground, and forms the mixing that particle diameter is 0.05-0.1mm and does
Powder powder；30-50 parts of ether dissolution will be added in mixed powder powder, remove undissolved impurity and obtain, and ether is steamed
Send out the solid matter after being evaporated.Earthworm peptide in dried rainworm can stimulate the secretion of cellular matrix collagen, but contain
The excessive easily skin irritatin to face is measured, therefore the dried rainworm of 30-35 parts of selection is very suitable.The boiling point of ether is only
34.6 DEG C, therefore the middle whitening active ingredient of dried rainworm and Schisandra chinensis is extracted by ether, whitening active ingredient will not be made
Into destruction.
The first paste, the second paste and step of step S6 of S8, mixed merga pass blender whipping step S3
Solid matter in S7, and 15-18 parts of emulsifying agent is added, whitening cream finished product is formed when stirring 2-4 is small, the speed of stirring is
700-800 revs/min, by the temperature control in blender at 12-18 DEG C.The speed of stirring is 700-800 revs/min, will be stirred
The temperature control in device is mixed at 12-18 DEG C, whitening composition can be produced to avoid waste heat caused by the excessive velocities of stirring
It is raw to destroy.
In the preparation method of the whitening cream of the present invention for being based on natural extract, after step s8
Further include：
Whitening cream finished product, be positioned in sealing container by S9, and the condition in sealing container is as follows：Oxygen content in air
Control is passed through helium and is maintained at a standard atmospheric pressure 2% to 3%, and atmospheric pressure error is empty within positive and negative 5 percent
For gas relative humidity in 40%-45%, temperature scope is 12-18 DEG C.This step substitutes oxygen by using this inert gas of helium
Gas, can avoid the cost-effectively in whitening cream from being failed by oxidation.
S10, by ultraviolet sterilize sealing container 60-120 minute, and the intensity of ultraviolet disinfection is every square centimeter
100-110 microwatts.This step avoids the growth of microorganism, and whitening cost-effectively is damaged.Preferably, can also pass through
Sealing inner wrapping carries out the finished product of whitening cream to separate packaging, and extracts the air in packaging.
In the preparation method of the whitening cream of the present invention based on natural extract,
Trans 2.5-2.6 parts of dioxy morin, 4-8 parts of motherwort powder, sub- oil are additionally added when being stirred in the step S8
It is 1.3-1.7 parts sour.
It is understood that for those of ordinary skill in the art, it can be conceived with the technique according to the invention and done
Go out other various corresponding changes and deformation, and all these changes and deformation should all belong to the protection model of the claims in the present invention
Enclose.
Claims (1)
1. a kind of preparation method of the whitening cream based on natural extract, it is characterised in that it includes the following steps：It is following
The number that step occurs is weight ratio,
S1, the purple grape for taking 50-80 parts, 50-100 parts of blueberry, 40-60 parts of mulberries, are placed in container, and add 100-
160 parts of water, is stirred mashing by mixer and forms pulp mixed liquor in 30-60 minutes, by temperature control at 4-6 DEG C, turn
Speed is 400-450 revs/min；
S2, the pulp mixed liquor to purple grape, blueberry and mulberries carry out traditional vacuum and handle to obtain slurries, for mixing fruit again
Starch mixed liquor and remove the pulp slag of purple grape, blueberry and mulberries；3-4 parts of cellulases are added into slurries to be digested,
At 22-25 DEG C, slurries digest 5-10 days the temperature of enzymolysis, are less than 3% to remaining monose, separation tank switching obtains zymotic fluid；
S3, by zymotic fluid stand 4-7 it is small when, temperature control is at 4-6 DEG C；Take the supernatant liquor of the zymotic fluid after standing；By upper strata
Clear liquid is transferred to container for evaporation and carries out heating evaporation, by the pressure control in container for evaporation in 1800 to 2600 pas, until evaporating
Water content drops to 10-15% the first pastes of formation in supernatant liquor in container；
S4, take out the first paste and be put into spare in storage container, by the temperature control in storage container at 12-18 DEG C, deposits
Oxygen content in reservoir ware air is controlled 2% to 3%；
S5, the haematococcus pluvialis for taking 30-60 parts, and haematococcus pluvialis are placed 3-5 days at 26-28 DEG C of shady and cool ventilation, take
Haematococcus pluvialis and reticulate millettia mixed grinding are formed the mixed-powder that particle diameter is 0.05-0.1mm by 40-50 parts of reticulate millettia；Will
Add 80-100 part of water dissolving in mixed-powder, and after traditional vacuum is handled with the filter membrane that aperture is 0.30-0.35 μm into
Haematococcus pluvialis and the hybrid filtering liquid of reticulate millettia are obtained by filtration in row；
S6, adjust the pH value of haematococcus pluvialis and the hybrid filtering liquid of reticulate millettia to being with aperture again after 7.15-7.25
0.30-0.35 μm of filter membrane carries out that cleaner liquid was obtained by filtration, and carries out heating evaporation to crossing cleaner liquid by container for evaporation, will steam
The pressure control in container is sent out in 1000 to 1400 pas, until the water content in cleaner liquid of crossing in container for evaporation drops to 20-
35% forms the second paste；
S7, take 30-35 parts of dried rainworms and 2-3 portions of Schisandra chinensis to be ground, and forms the mixed powder powder that particle diameter is 0.05-0.1mm
End；30-50 parts of ether dissolution will be added in mixed powder powder, remove undissolved impurity and obtain, and ether is evaporated
Solid matter after to evaporation；
S8, the first paste of mixed merga pass blender whipping step S3, step S6 the second paste and step S7 in
Solid matter, and add 15-18 parts of emulsifying agent, whitening cream finished product formed when stirring 2-4 is small, the speed of stirring is 700-
800 revs/min, by the temperature control in blender at 12-18 DEG C；
Further include after step s8：
Whitening cream finished product, be positioned in sealing container by S9, and the condition in sealing container is as follows：Oxygen content control in air
2% to 3%, and it is passed through helium and is maintained at a standard atmospheric pressure, atmospheric pressure error is within positive and negative 5 percent, air phase
To humidity in 40%-45%, temperature scope is 12-18 DEG C；
S10, by ultraviolet to sealing container sterilize 60-120 minutes, the intensity of ultraviolet disinfection is 100- every square centimeter
110 microwatts；
Trans 2.5-2.6 parts of dioxy morin, 4-8 parts of motherwort powder, linoleic acid are additionally added when being stirred in the step S8
1.3-1.7 part.
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| CN113855620B (en) * | 2021-11-24 | 2023-08-25 | 安赛搏(重庆)生物技术有限公司 | Composition containing saussurea involucrata cell culture and preparation method and application thereof |
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| CN104688615A (en) * | 2014-09-06 | 2015-06-10 | 杭州诺丽生物技术有限公司 | Enzyme facial mask essence with moisturizing and whitening functions |
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