CN105327331A - Composition for treating and preventing senile dementia and preparation method and application of composition for treating and preventing senile dementia - Google Patents
Composition for treating and preventing senile dementia and preparation method and application of composition for treating and preventing senile dementia Download PDFInfo
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Abstract
The invention discloses a composition for treating and preventing senile dementia. The composition is composed of rice bran polysaccharide, rice polypeptide and sesame sprout extract. The sesame sprout extract is prepared by using a mixed solvent with ethyl alcohol and water in a proportion of 60-80:100 to extract sesame sprouts, wherein a ratio of each-time mixed solvent consumption to sesame sprout weight is 8-15:1, and extraction conditions include: keeping a slight boiling state for 1-2h, extracting for 1-3 times, combining extract liquid, concentrating to 0.5-1g/ml, cooling, standing to the room temperature, adding ethyl alcohol while stirring until the concentration of the ethyl alcohol in a final system is 60-80%, filtering, concentrating filtrate, and drying to obtain the extract. The invention further discloses a preparation method and application of the composition for treating and preventing senile dementia. Active ingredients in the sesame sprouts are extracted and compounded with the rice bran polysaccharide and the rice polypeptide to form the composition which has functions of treating and preventing senile dementia.
Description
Technical field
The invention belongs to integration of edible and medicinal herbs technical field, be specifically related to a kind of composition and method of making the same and the application that are used for the treatment of and prevent senile dementia.
Background technology
Along with the development of society, the prolongation of human longevity, social senilization's phenomenon is day by day serious, and the prevalence of alzheimer disease obviously raises, and has become and has caused one of main causes of death.Alzheimer disease is a kind of progressive neurodegenerative diseases, and main manifestations is the neuropsychic symptoms such as gradual memory obstacle, cognitive dysfunction, personality changes and aphasis, has a strong impact on social activity, occupation and life.Main manifestations is maincenter cognitive dysfunction clinically, and a few neurofibrillary tangle of senile plaque, amyloid beta deposition is the key pathological feature of brain.
Existing about 3,100,000 patients with Alzheimer diseases of China according to statistics, over-65s prevalence is up to 5.9%.Clinical symptoms is Progressive symmetric erythrokeratodermia cognitive dysfunction, and to end-stage disease, minimal invasive treatment can not take care of oneself, mute, and even the close relative of oneself is not familiar with.This brings very large misery not only to patient self, meanwhile, causes great burden also to household and society, impact society and expanding economy.
Because what pathogenesis of its cause of disease is still not clear, do not have effect method to reverse at present and stop disease progression.But carry out symptomatic treatment in early days, comprise Drug therapy or food treatment improves cognitive function, improves mental symptom, psychosocial therapy and good nursing, the quality of life delaying patient is gone down very important.The medicine that domestic and international approval is used for senile dementia disease treatment mainly contains (1) cholinesterase inhibitor, and common drug has donepezil, galantamine etc.(2) glutamate receptor antagonists, common drug has memantine.But the effect of said medicine treatment alzheimer disease is single, and therapeutic effect is limited, has certain side effect.Therefore, the natural safe health food of research and development control senile dementia becomes research topic important now.
Up to now, rice bran polysaccharide (RBP), large mpd polypeptide and the Semen Sesami bud extract research report at treatment senile dementia is had no.
Summary of the invention
A technical problem to be solved by this invention is for providing a kind of compositions being used for the treatment of and preventing senile dementia, and said composition comprises rice bran polysaccharide (RBP) and large mpd polypeptide, and Semen Sesami bud extract, has good effect for prevention and therapy senile dementia.
Second technical problem to be solved by this invention is for providing the preparation method of said composition.
3rd technical problem to be solved by this invention is for providing said composition in preparation treatment and the application preventing senile dementia medicine.
For solving above-mentioned first technical problem, the compositions designed by the present invention comprises rice bran polysaccharide (RBP), large mpd polypeptide and Semen Sesami bud extract.
Described Semen Sesami bud extract prepares through following method:
The mixed solvent being 60 ~ 80:100 by Semen Sesami bud ethanol and water ratio extracts, each mixed solvent consumption and Semen Sesami bud weight ratio are 8 ~ 15:1, and extraction conditions is: keep slight boiling condition 1 ~ 2h, extract 1 ~ 3 time, merge extractive liquid, be concentrated into 0.5 ~ 1g Semen Sesami bud/ml, cool, leave standstill to room temperature, adding concentration of alcohol in ethanol to end-body system under stirring is 60 ~ 80%, filter, filtrate concentrates, dry, obtains extractum.
Preferably, in compositions by weight, rice bran polysaccharide (RBP) 10 ~ 50 parts, large mpd polypeptide 10 ~ 50 parts, Semen Sesami bud extract 10 ~ 50 parts.More preferably, rice bran polysaccharide (RBP) 10 ~ 20 parts, large mpd polypeptide 30 ~ 60 parts, Semen Sesami bud extract part 30 ~ 50.
Preferably, in Semen Sesami bud extract preparation process, ethanol and water ratio are 65 ~ 75:100, and each mixed solvent consumption and Semen Sesami bud weight ratio are 9 ~ 12:1.
Preferably, in Semen Sesami bud extract preparation process, cool, leave standstill to room temperature, adding concentration of alcohol in ethanol to end-body system under stirring is 70 ~ 75%, filters, and filtrate concentrates, dry, obtains extractum.
For solving the present invention's second technical problem, the preparation method of compositions provided by the invention is:
By rice bran polysaccharide (RBP), large mpd polypeptide and Semen Sesami bud extract mix homogeneously, form extractum, obtain the compositions being used for the treatment of and preventing senile dementia of the present invention.
For solving the present invention's the 3rd technical problem, the present invention also provides said composition in preparation treatment and the application preventing senile dementia medicine.When for the preparation for the treatment of and prevention senile dementia medicine, adjuvant separately or can be added and prepare solid dosage forms as pill, electuary, adjuvant also separately or can be added and prepare semisolid dosage form as soft extract, also can prepare liquid dosage form as oral liquid.Adjuvant can be glyceryl monostearate, Arlacel-80, soybean oil, Cera Flava, gelatin, glycerol, water, titanium dioxide; Also can be those skilled in the art be other additives preparing solid, semisolid or liquid dosage form.
The present invention is by extracting the active component in Semen Sesami bud, and this active component and rice bran polysaccharide (RBP), large mpd polypeptide is composite, and three can occur to work in coordination with the effect playing prevention and therapy senile dementia.The pill zoopery prepared by said composition confirms, said composition obviously can improve the learning disorder that hydrocortisone, scopolamine or sodium nitrite cause mice dementia animal model; Ethanol can be improved and cause the effect of mouse memory reproduction disappearance.In addition, said composition also has the effect of prevention and therapy vascular dementia.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further detailed explanation.
Compositions designed by the present invention comprises rice bran polysaccharide (RBP), large mpd polypeptide and Semen Sesami bud extract.Prepare through following method for the rice bran polysaccharide (RBP) in the specific embodiment of the invention: defatted rice bran aqua calcis is extracted, each water consumption is rice bran meal 8 ~ 15 times, aqua calcis concentration 0.1%, extraction time 3h, liquid ratio 15:1, extract pressure 145kPa, extract 2 times, merge extractive liquid, obtains rice bran polysaccharide (RBP) through membrance separation, concentrating under reduced pressure, vacuum drying.Large mpd polypeptide in the specific embodiment of the invention prepares through following method: by rice protein alkaline protease enzymolysis, enzymatic hydrolysis condition is: rice protein is dissolved in water with 10% concentration, hydrolysis temperature 50 DEG C, enzymolysis time 4.5h, pH10, enzymolysis solution obtains Rice Bran Polypeptides through membrance separation, concentrating under reduced pressure, vacuum drying.Semen Sesami bud extract in the specific embodiment of the invention prepares through following method: by Semen Sesami bud extraction step, ethanol and water ratio are 65 ~ 75:100, each mixed solvent consumption and Semen Sesami bud weight ratio are 9 ~ 12:1, extraction conditions is: keep slight boiling condition 1 ~ 2h, extract 1 ~ 3 time, merge extractive liquid, be concentrated into 0.5 ~ 1g Semen Sesami bud/ml, cool, leave standstill to room temperature, adding concentration of alcohol in ethanol to end-body system under stirring is 70 ~ 75%, filters, and filtrate concentrates, drying, obtains extractum.
For convenience of application, usually above-mentioned composition is processed into dosage form, concrete formulation method is shown in embodiment 1 ~ 3.
Embodiment 1
A, to prepare burden according to following masses part:
Rice bran polysaccharide (RBP) 10 parts
Large mpd polypeptide 40 parts
Semen Sesami bud extract 50 parts
B, by after each component mix homogeneously of steps A, add the aqueous solution of porous-starch, stir, vibration absorption 60min under 30 DEG C of conditions; Solution is mixed homogeneously with sodium alginate soln, and in the calcium chloride solution of mixed solution instillation 1.5%, solidification 30min, filters microcapsule, with the calcium chloride that water clean surface is residual, and obtained microscapsule powder after dry.
Wherein rice bran polysaccharide (RBP), large mpd polypeptide and Semen Sesami bud extract extract through following conventional methods:
A, by rice protein alkaline protease enzymolysis, rice protein is dissolved in water with 10% concentration, hydrolysis temperature 50 DEG C, enzymolysis time 4.5h, pH10, and enzymolysis solution obtains large mpd polypeptide through membrance separation, concentrating under reduced pressure, vacuum drying;
B, defatted rice bran aqua calcis to be extracted, each water consumption be rice bran meal 8-15 doubly, aqua calcis concentration 0.1%, extraction time 3h, liquid ratio 15:1, extract pressure 145kPa, extract 2 times, merge extractive liquid, obtains rice bran polysaccharide (RBP) through membrance separation, concentrating under reduced pressure, vacuum drying.
C, by Semen Sesami bud 70% ethanol extraction, each solvent load is Semen Sesami bud 12 times, each extraction time, keep slight boiling condition 1h, extract 2 times, merge extractive liquid, be concentrated into 1g Semen Sesami bud/ml, cool, leave standstill to room temperature, adding ethanol to final concentration under stirring is 70%, filters, and filtrate concentrates, drying, obtains extractum.
Embodiment 2
A, to prepare burden according to following masses part:
Rice bran polysaccharide (RBP) 30 parts
Large mpd polypeptide 50 parts
Semen Sesami bud extract 10 parts
B, by after each component mix homogeneously of steps A, add glyceryl monostearate, Arlacel-80 %, soybean oil, Cera Flava obtain stable content; The capsule skin of gelatin, glycerol, water, titanium dioxide, prepares seamless soft capsule by dropping preparation method.
Wherein rice bran polysaccharide (RBP), large mpd polypeptide and Semen Sesami bud extract extract through following conventional methods:
A, by rice protein alkaline protease enzymolysis, rice gluten is dissolved in water with 10% concentration, hydrolysis temperature 50 DEG C, enzymolysis time 4.5h, pH10, and enzymolysis solution obtains large mpd polypeptide through membrance separation, concentrating under reduced pressure, vacuum drying;
B, defatted rice bran aqua calcis to be extracted, each water consumption be rice bran meal 8-15 doubly, aqua calcis concentration 0.1%, extraction time 3h, liquid ratio 15:1, extract pressure 145kPa, extract 2 times, merge extractive liquid, obtains rice bran polysaccharide (RBP) through membrance separation, concentrating under reduced pressure, vacuum drying.
C, by Semen Sesami bud 75% ethanol extraction, each solvent load is Semen Sesami bud 8 times, each extraction time, keep slight boiling condition 2h, extract 2 times, merge extractive liquid, be concentrated into 0.5g Semen Sesami bud/ml, cool, leave standstill to room temperature, adding ethanol to final concentration under stirring is 75%, filters, and filtrate concentrates, drying, obtains extractum.
Embodiment 3
A, to prepare burden according to following masses part:
Rice bran polysaccharide (RBP) 50 parts
Large mpd polypeptide 10 parts
Semen Sesami bud extract 30 parts
B, by after each component mix homogeneously of steps A, add 0.15% potassium sorbate and 2% oligomeric isomaltose to add water stirring standardize solution, the centrifugal 10min of 3000r/min, standardize solution obtains oral liquid finished product.
Wherein rice bran polysaccharide (RBP), large mpd polypeptide and Semen Sesami bud extract extract through following conventional methods:
A, by rice protein alkaline protease enzymolysis, rice gluten is dissolved in water with 10% concentration, hydrolysis temperature 50 DEG C, enzymolysis time 4.5h, pH10, and enzymolysis solution obtains large mpd polypeptide through membrance separation, concentrating under reduced pressure, vacuum drying;
B, defatted rice bran aqua calcis to be extracted, each water consumption be rice bran meal 8-15 doubly, aqua calcis concentration 0.1%, extraction time 3h, liquid ratio 15:1, extract pressure 145kPa, extract 2 times, merge extractive liquid, obtains rice bran polysaccharide (RBP) through membrance separation, concentrating under reduced pressure, vacuum drying.
C, by Semen Sesami bud 65% ethanol extraction, each solvent load is Semen Sesami bud 15 times, each extraction time, keep slight boiling condition 2h, extract 1 time, merge extractive liquid, be concentrated into 1g Semen Sesami bud/ml, cool, leave standstill to room temperature, adding ethanol to final concentration under stirring is 72%, filters, and filtrate concentrates, drying, obtains extractum.
For showing anti-senile dementia effect of compositions of the present invention, proved by following zoopery:
1. experimental technique
1.1 key instruments and reagent
Water maze monitoring system is purchased from institute of Materia Medica,Chinese Academy of Medical Sciences; Balance is Sartorius Products, and Rotary Evaporators is purchased from Buchi company of Switzerland, and vacuum drying oven is purchased from Shanghai Bai Xin instrument and equipment factory), high speed disintegrator (HY state 8) is purchased from Beijing Huan Ya Tianyuan Mechanical Technology Inc.; PHS-2C type precision acidity meter Shanghai Lei Ci instrument plant, RE52-99 type Rotary Evaporators Shanghai Yarong Biochemical Instrument Plant, LXJ-II type centrifugal precipitation mechanism Shanghai medical analytical instrument factory, SHB-III type circulating water type multiplex vacuum pump Zhengzhou Great Wall science, industry and trade company.
Be used for the treatment of and prevent the impact of combination dosage form on Model of Dementia of senile dementia in 1.2 embodiment of the present invention 1
Behaviors survey
Morris water maze is tested, and Morris water maze is made up of a round pool and automatic video recording and analytical system two parts.Pond height 70cm, diameter 100cm, one of them quadrant central authorities placed by lucite platform, and water temperature controls at (22 ± 2 DEG C).Animal trains 4 times every day, trains 5d altogether.Data sampling and processing is completed by image automatic monitoring and processing system, the 4th day, from first, second and third quadrant place of entry, animal is put into water towards pool wall successively, records animal escape latency and swim across to obtain distance in water respectively.
Mice passive avoidance Jumping test
Device is the tawny organic glass case of 80cm × 16cm × 40cm, is covered with copper grid as stimulating electrode, inside establishes high 4.5cm at the bottom of case, and the valve rubber of diameter 6.5cm is as the security platform avoiding electric shock.First put into mice and adapt to 5min, with 36V alternating current electric shock mice, if it is correct that mice jumps onto security platform, as jumped off from platform, biped contact copper grid are wrong reaction.The wrong reaction number occurred in record 3min.Resurvey after 24h and test, with mice study and resurvey next day the wrong reaction number of times and latent time testing and occur in (memory) process to evaluate ability of learning and memory.
1.2.1 to the effect of ethanol interference in learning Memory Process
Mice 90, random point 6 groups (positive controls (is called for short positive group, piracetam 400mg/kg), model group (ethanol group), Testa oryzae glycopeptide group (rice bran polysaccharide (RBP) and large mpd polypeptide weight ratio are the mixture of 1:4), be used for the treatment of in the embodiment of the present invention 1 and prevent the combination dosage form of senile dementia (hereinafter referred to as compositions) (high dose group, middle dosage group and low dose group), often organizing 15.Ethanol group oral normal saline, piracetam group gavages piracetam 400mg/kg, Testa oryzae glycopeptide group and combination object height, in and low dose group gavage Testa oryzae glycopeptide 400mg/kg respectively, compositions 400mg/kg, 200mg/kg, 100mg/kg, successive administration 11d, during 7-10d, every day, 1h carried out labyrinth after administration, continuous training 4d, before 11d training, ethanol group gavages the ethanol 5mL/kg that volume fraction is 50%, piracetam group gavages piracetam 400mg/kg after gavaging 50% ethanol 5mL/kg immediately, Testa oryzae glycopeptide and compositions high dose, middle dosage and low dose group gavage Testa oryzae glycopeptide 400mg/kg immediately after gavaging 50% ethanol 5mL/kg, compositions 400mg/kg, 200mg/kg, 100mg/kg, after administration, 1h tests.
1.2.2 to the effect of scopolamine interference in learning Memory Process
Mice 105, random point 7 groups (normal saline group, model group (scopolamine group), piracetam group, Testa oryzae glycopeptide group, compositions high dose group, middle dosage group and low dose group), often organizes 15.Normal saline group (i.e. Normal group) and the oral normal saline of model group; The oral piracetam 600mg/kg of piracetam group; Testa oryzae glycopeptide group oral Testa oryzae glycopeptide 400mg/kg, combination object height, in and low dose group respectively Orally administered composition 400mg/kg, 200mg/kg, 100mg/kg, successive administration 11d, during 6-10d, every day, mice was put into diving tower instrument capsule endoadaptation 3min by 1h respectively after administration, and every capsule puts mice 1, connect 36V power supply, training 5min.Continuous training 5d, before 11d training, normal saline group and model group still gavage normal saline; Piracetam group, Testa oryzae glycopeptide group, compositions high dose, middle dosage and low dose group are respectively at after gavage 30min, except normal saline group gavages except normal saline, all the other each group scopolamine hydrobromide normal saline solution gavaging 4mg/kg, after end, mice is transferred to diving tower instrument place laboratory to conform, after administration, 1h tests.
1.2.3 the effect that mouse memory consolidates obstacle is caused to sodium nitrite
Mice 105, random point 7 groups (normal saline group, model group (sodium nitrite group), piracetam group, Testa oryzae glycopeptide group, compositions high dose group, middle dosage group and low dose group), often organizes 15.Normal saline group and the oral normal saline of model group; Matched group (piracetam group) oral piracetam 600mg/kg; Testa oryzae glycopeptide group oral Testa oryzae glycopeptide 400mg/kg, combination object height, in and low dose group respectively Orally administered composition 400,200,50mg/kg, successive administration 11d, during 6-10d every day 1h after administration, mice is put into respectively diving tower instrument capsule endoadaptation 3min, connect 36V power supply, training 5min.Continuous training 5d, after 11d training, normal saline group and model group still gavage normal saline; Matched group, Testa oryzae glycopeptide group, compositions high dose, middle dosage and low dose group are respectively at after gavage 30min, and except blank group intraperitoneal injection of saline, all the other respectively organize lumbar injection sodium nitrite 120mg/kg normal saline solution; Retest mouse memory situation at 12d, observe and record errors number in incubation period and 5min that mice jumps off first.
1.2.4 hydrocortisone is caused to the effect of mice with kidney-yang deficiency learning and memory function
Mice 105, by body weight, mice is divided into random point 7 groups of (normal saline group, model group (hydrocortisone group), JINGUI SHENQI WAN group (Beijing Tongrentang Technology Development Co.ltd. Pharmaceutical Factory), Testa oryzae glycopeptide groups at random, compositions high dose group, middle dosage group and low dose group), often organize 15.Often organize 15, male and female half and half.JINGUI SHENQI WAN group, Testa oryzae glycopeptide group, compositions high dose group, middle dosage group and low dose group, gastric infusion, normal saline group and the oral normal saline of model group; , matched group (JINGUI SHENQI WAN group) oral JINGUI SHENQI WAN 500mg/kg; Testa oryzae glycopeptide group oral Testa oryzae glycopeptide 400mg/kg, combination object height, in and low dose group respectively Orally administered composition 400,200,50mg/kg, successive administration 19d.In the 11st day of experiment except normal group, each group gavage hydrocortisone 1mg/, successive administration 8d.In the 19d of experiment, 1h after administration, puts into the reaction chamber endoadaptation environment 3min of diving tower instrument by mice, then pass to the alternating current of 36V immediately, after mice is shocked by electricity, majority is jumped onto diving tower and is escaped electric shock, mice from diving tower jump off shocked by electricity after rebound diving tower again, training 5min.Be put in by mice on diving tower, record mice first time jumps off the errors number in time (as incubation period) of diving tower and 5min.
2. result
2.1 impacts on ethanol interference in learning Memory Process
Table 1 compositions and other group are on the impact of ethanol interference in learning Memory Process
It is close that each treated animal gives the response time of water maze before ethanol, do not have significant difference between each group.After giving ethanol, the ethanol group reaction time obviously extends, and display mice has occurred that learning and memory representational role lacks, model copy success.After giving ethanol, each dosage group of compositions, Testa oryzae glycopeptide group and positive group reaction time are all less than ethanol group, have significant difference between compositions high dose group and ethanol group.Show that compositions is reproduced disappearance to alcohol induced learning and memory of little mouse and had significant protective effect, and have obvious dose-dependence, waiting under dosage effect, the protective effect of compositions is significantly better than piracetam and Testa oryzae glycopeptide.
2.2 impacts on scopolamine interference in learning Memory Process
Table 2 compositions and other group are on the impact of scopolamine interference in learning Memory Process
Compare with model group, compositions 100mg/kg, 200mg/kg, 400mg/kg group, Testa oryzae glycopeptide group and piracetam group all obviously extend incubation period, and errors number obviously reduces; Compositions 100mg/kg group also obviously extends incubation period, but errors number only has minimizing to a certain degree.Testa oryzae glycopeptide improves significantly to the learning and memory of little mouse acquired disturbance caused by scopolamine, and waiting under dosage effect, the improvement result of compositions is better than Testa oryzae glycopeptide.
2.3 cause on sodium nitrite the impact that mouse memory consolidates obstacle
Compare with model group, compositions 100mg/kg, 200mg/kg, 400mg/kg group all obviously extend incubation period, and errors number has minimizing to a certain degree; Compositions 100mg/kg, 200mg/kg, 400mg/kg group incubation period and errors number have improvement to a certain degree; Piracetam group obviously extends incubation period, and errors number significantly reduces.Conclusion: compositions is consolidated obstacle to sodium nitrite induced mice learning and memory and improved significantly, and the improvement result of compositions is better than Testa oryzae glycopeptide.
Table 3 compositions and other group cause on sodium nitrite the impact that mouse memory consolidates obstacle
2.4 cause the impact of mice with kidney-yang deficiency learning and memory function to hydrocortisone
Table 4 compositions and other group cause the impact of mice with kidney-yang deficiency learning and memory function to hydrocortisone
Model group after giving hydrocortisone 8d, compare with normal group obviously shorten the incubation period that diving tower tests, errors number obviously increases, and illustrates that damage in learning and memory appears in model mice.JINGUI SHENQI WAN group, Testa oryzae glycopeptide group and each dosage group of compositions are after giving hydrocortisone 8d, compare with model group and obviously extend the incubation period that diving tower tests, errors number obviously reduces, JINGUI SHENQI WAN is described, Testa oryzae glycopeptide and compositions all can resist the learning and memory of little mouse dysfunction that hydrocortisone causes, and the antagonism of compositions is better than Testa oryzae glycopeptide group and JINGUI SHENQI WAN group to a certain extent, waiting under dosage effect, the improvement result of compositions is significantly better than Testa oryzae glycopeptide.
Inventor has carried out the impact test of Model of Dementia simultaneously on the compositions and other dosage forms of not carrying out formulation, confirm that the dosage form of the compositions and other dosage forms and embodiment 1 of not carrying out formulation has similar action effect, Testa oryzae glycopeptide group, piracetam group and JINGUI SHENQI WAN group are obviously better than to the effect of prevention and therapy senile dementia aspect.
Claims (8)
1. be used for the treatment of and prevent a compositions for senile dementia, it is characterized in that:
Said composition comprises rice bran polysaccharide (RBP) and large mpd polypeptide, and Semen Sesami bud extract, and described Semen Sesami bud extract prepares through following method:
The mixed solvent being 60 ~ 80:100 by Semen Sesami bud ethanol and water ratio extracts, each mixed solvent consumption and Semen Sesami bud weight ratio are 8 ~ 15:1, extraction conditions: keep slight boiling condition 1 ~ 2h, extract 1 ~ 3 time, merge extractive liquid, be concentrated into 0.5 ~ 1g Semen Sesami bud/ml, cool, leave standstill to room temperature, adding concentration of alcohol in ethanol to end-body system under stirring is 60 ~ 80%, filters, and filtrate concentrates, drying, obtains extractum.
2. the compositions being used for the treatment of and preventing senile dementia according to claim 1, is characterized in that, in described compositions, by weight: rice bran polysaccharide (RBP) 10 ~ 50 parts, large mpd polypeptide 10 ~ 50 parts, Semen Sesami bud extract 10 ~ 50 parts.
3. the compositions being used for the treatment of and preventing senile dementia according to claim 1 and 2, is characterized in that, rice bran polysaccharide (RBP) 10 ~ 20 parts, large mpd polypeptide 30 ~ 60 parts, Semen Sesami bud extract part 30 ~ 50.
4. the compositions being used for the treatment of and preventing senile dementia according to claim 1 and 2, it is characterized in that, in described Semen Sesami bud extract preparation process: ethanol and water ratio are 65 ~ 75:100, each mixed solvent consumption and Semen Sesami bud weight ratio are 9 ~ 12:1.
5. the compositions being used for the treatment of and preventing senile dementia according to claim 3, is characterized in that, in described Semen Sesami bud extract preparation process: ethanol and water ratio are 65 ~ 75:100, each mixed solvent consumption and Semen Sesami bud weight ratio are 9 ~ 12:1.
6. the compositions being used for the treatment of and preventing senile dementia according to claim 3, is characterized in that, in described Semen Sesami bud extract preparation process: cool, leave standstill to room temperature, adding concentration of alcohol in ethanol to end-body system under stirring is 70 ~ 75%.
7. be used for the treatment of and prevent the preparation method of compositions for senile dementia, it is characterized in that, the method is:
Rice bran polysaccharide (RBP), Rice Bran Polypeptides and Semen Sesami bud extract mix homogeneously are formed extractum, and described Semen Sesami bud extract is prepared by following method:
The mixed solvent being 60 ~ 80:100 by Semen Sesami bud ethanol and water ratio extracts, each mixed solvent consumption and Semen Sesami bud weight ratio are 8 ~ 15:1, extraction conditions: keep slight boiling condition 1 ~ 2h, extract 1 ~ 3 time, merge extractive liquid, be concentrated into 0.5 ~ 1g Semen Sesami bud/ml, cool, leave standstill to room temperature, adding concentration of alcohol in ethanol to end-body system under stirring is 60 ~ 80%, filter, filtrate concentrates, dry, obtains extractum.
8. the compositions described in any one of claim 1 ~ 6 is in preparation treatment and the application preventing senile dementia medicine.
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| CN106432529A (en) * | 2016-11-24 | 2017-02-22 | 武汉轻工大学 | Preparation method of high-purity rice bran polysaccharide |
| CN109907319A (en) * | 2019-03-12 | 2019-06-21 | 中南林业科技大学 | A kind of rice bran extract and preparation method thereof and the application in health food |
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| CN106432529A (en) * | 2016-11-24 | 2017-02-22 | 武汉轻工大学 | Preparation method of high-purity rice bran polysaccharide |
| CN106432529B (en) * | 2016-11-24 | 2019-01-08 | 武汉轻工大学 | A kind of preparation method of high-purity rice bran polysaccharide |
| CN109907319A (en) * | 2019-03-12 | 2019-06-21 | 中南林业科技大学 | A kind of rice bran extract and preparation method thereof and the application in health food |
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