CN105325291A - Weeping oriental cherry tissue culture method - Google Patents
Weeping oriental cherry tissue culture method Download PDFInfo
- Publication number
- CN105325291A CN105325291A CN201510621194.1A CN201510621194A CN105325291A CN 105325291 A CN105325291 A CN 105325291A CN 201510621194 A CN201510621194 A CN 201510621194A CN 105325291 A CN105325291 A CN 105325291A
- Authority
- CN
- China
- Prior art keywords
- culture
- pendula
- bean
- medium
- tissue culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000014001 Prunus serrulata Nutrition 0.000 title abstract description 15
- 241000392970 Prunus serrulata Species 0.000 title abstract description 15
- 238000012136 culture method Methods 0.000 title abstract description 4
- 238000000034 method Methods 0.000 claims abstract description 26
- 238000002360 preparation method Methods 0.000 claims abstract description 3
- 241001065644 Ficus asperifolia Species 0.000 claims description 30
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 30
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 30
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 19
- 229930006000 Sucrose Natural products 0.000 claims description 19
- 239000005720 sucrose Substances 0.000 claims description 19
- 229920001817 Agar Polymers 0.000 claims description 18
- 239000008272 agar Substances 0.000 claims description 18
- 238000000338 in vitro Methods 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 7
- 230000032683 aging Effects 0.000 abstract description 3
- 230000004069 differentiation Effects 0.000 abstract description 3
- 230000000977 initiatory effect Effects 0.000 abstract 2
- 238000011109 contamination Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 17
- 230000012010 growth Effects 0.000 description 15
- 239000000463 material Substances 0.000 description 11
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 229930191978 Gibberellin Natural products 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 3
- 239000003448 gibberellin Substances 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 241000167854 Bourreria succulenta Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 235000019693 cherries Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 235000003325 Ilex Nutrition 0.000 description 1
- 241000209035 Ilex Species 0.000 description 1
- 240000007189 Prunus angustifolia Species 0.000 description 1
- 235000010873 Prunus angustifolia Nutrition 0.000 description 1
- 235000005706 Prunus prostrata Nutrition 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 description 1
- RRGUKTPIGVIEKM-UHFFFAOYSA-N cilostazol Chemical compound C=1C=C2NC(=O)CCC2=CC=1OCCCCC1=NN=NN1C1CCCCC1 RRGUKTPIGVIEKM-UHFFFAOYSA-N 0.000 description 1
- 229960004588 cilostazol Drugs 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a weeping oriental cherry tissue culture method including the steps: preparation of an explant, initiation culture, multiplication culture and rooting culture; a culture medium of the multiplication culture contains 0.2-0.5 mg/L of 6-BA, 0.03-0.07 mg/L of NAA and 5-15 mg/L of GA. The weeping oriental cherry tissue culture method has the advantages of lower contamination rate and good starting effect; the induced differentiation rate of the initiation culture can reach 84%; in the multiplication culture stage, weeping oriental cherry can perform normal jointing, tissue-cultured seedlings grow healthy and strong, the problems of shortened internode and easy aging generally appearing in a traditional oriental cherry tissue culture process are solved, and the multiplication coefficient can reach 7.0.
Description
Technical field
The present invention relates to field of plant tissue culture technique, particularly relate to a kind of method for tissue culture of F. pendula Bean.
Background technology
The traditional modes of reproduction of oriental cherry is propagation by grafiting, but its seedling is slow, is difficult to produce fast in a large number.Along with the development of tissue culture technique, tissue cultures is that the Fast-propagation of oriental cherry and factorial praluction provide an effective way.Domestic scholars mainly concentrates in the kinds such as underbrown japanese cherry, Tokyo oriental cherry, Cyclobanopsis chungii, wild mountain cherry and kind the research of oriental cherry.F. pendula Bean be just grow up in recent years view and admire oriental cherry, hang down because its branch is soft elegant, attractive in appearance, be more and more applied to afforestation and in beautifying.
Research for F. pendula Bean tissue cultures aspect mainly contains: the flourish Chinese ilex of Nanjing Forestry University etc. reports the tissue culture technology of weeping branch cherry morning " red branch hangs down ", take stem with bud as explant, Primary culture base is MS+BA1.0mg/L+NAA0.1mg/L, and the inductivity of bud is 80%; Proliferated culture medium is MS+BA0.5mg/L+NAA0.1mg/L+ sucrose 35g/L, and growth coefficient is 4.15; Root media is 1/2MS+NAA1.0mg/L+IBA1.0mg/L, rooting rate reach 95% (Rong Dongqing, Wang Xianrong. the tissue-culturing rapid propagation test of weeping branch early cherry " red branch hangs down ". forestry science and technology is developed, 2008).
The people such as Xu Zhaobo are also studied with regard to the Introduction Observation of F. pendula Bean and propagation technique; in propagation technique, tissue cultures is only studied with regard to the group training of the cold-resistant stock of F. pendula Bean; the propagating materials adopted is sleeping bud and bud of sprouting; but do not form whole plant (Xu Zhaobo in research; Chen Xiuyun etc. F. pendula Bean Introduction Observation and raising technology research. Laiyang Agricultural College journal, 2001).
In sum, the research report at present for the method for tissue culture of F. pendula Bean is less, and not system, and there are some not high problems of reproduction coefficient.Trace it to its cause, in the tissue culture procedures of oriental cherry, most of kind or kind all can run into internode and not extend, easily aging, or occur leaf rolling, the phenomenons such as vitrifying, only have this problem thoroughly to solve, and group Cilostazol just can be made to good effect.
Summary of the invention
The object of this invention is to provide a kind of method for tissue culture of F. pendula Bean, to overcome the shortened internodes existed in the tissue cultures of existing F. pendula Bean, the problem that growth coefficient is low.
For achieving the above object, the present invention is achieved by the following technical solutions:
The method for tissue culture of F. pendula Bean of the present invention, comprising: the preparation of explant, Primary culture, Multiplying culture and culture of rootage; The medium of described Multiplying culture contains 6-BA0.2 ~ 0.5mg/L, NAA0.03 ~ 0.07mg/L and GA5 ~ 15mg/L.
The present invention improves proliferation culture medium formula; ubiquitous shortened internodes in F. pendula Bean, problem that reproduction coefficient is low can be solved; preferably, the formula of the medium of described Multiplying culture is: MS+6-BA0.2 ~ 0.5mg/L+NAA0.03 ~ 0.07mg/L+GA5 ~ 15mg/L+ sucrose 30 ~ 40g/L+ agar 5 ~ 8g/L.
Further preferred, in the medium of described Multiplying culture, the concentration of the concentration of 6-BA to be the concentration of 0.3 ~ 0.5mg/L, NAA be 0.05mg/L, GA is 10mg/L.Further, in the medium of described Multiplying culture, the concentration of 6-BA is 0.3mg/L or 0.5mg/L.
Preferably, described explant is stem segment with axillary bud or stem apex, takes from the middle and upper part of the slightly branch of raw spring then.
After sterilization, prune the two ends otch of stem apex base portion, stem segment with axillary bud, the simple bud stem apex or the stem-segment with single bud that are trimmed to 1 ~ 1.5cm carry out Primary culture.
Preferably, the formula of the medium of described Primary culture is:
MS+BA0.4 ~ 0.6mg/L+NAA0.03 ~ 0.07mg/L+ sucrose 30 ~ 40g/L+ agar 5 ~ 8g/L.
Further preferred, in the medium of described Primary culture, the concentration of 6-BA is the concentration of 0.5mg/L, NAA is 0.05mg/L.
In proliferated culture medium and Primary culture base, the concentration of sucrose is specifically as follows 30g/L, and the concentration of agar is specifically as follows 6.5g/L.
Preferably, the method for described Multiplying culture comprises: Primary culture, after 20 ~ 30 days, gets the stem-segment with single bud of 1 ~ 2cm from plantlet in vitro, be vertically inoculated in medium and cultivate.
Compared with prior art; beneficial effect of the present invention is: the tissue culture method of F. pendula Bean of the present invention has lower pollution rate; start effective; the differentiation-inducing rate of Primary culture can reach 84%; the Multiplying culture stage can normal jointing, and plantlet in vitro robust growth, solves the shortened internodes generally occurred in traditional oriental cherry tissue culture procedures; easily aging problem, and growth coefficient can reach 7.0.
Accompanying drawing explanation
Fig. 1 is that embodiment 1 pair of explant carries out disinfection the picture of sterilizing;
Fig. 2 is the picture of embodiment 1 explant when being inoculated into Primary culture base;
Fig. 3 is the embodiment 1 Primary culture picture of 10 days;
Fig. 4 is the embodiment 1 Multiplying culture picture of 20 days;
Fig. 5 is the embodiment 1 Multiplying culture picture of 25 days;
Fig. 6 is the picture of embodiment 1 culture of rootage 20 days complete stools;
Fig. 7 is the picture of embodiment 1 culture of rootage 20 days roots;
Fig. 8 is that embodiment 2 adopts the medium only adding 6-BA0.5mg/L and NAA0.05mg/L two plant growth regulators to carry out the Multiplying culture picture of 20 days.
Embodiment
Below in conjunction with specific embodiment, illustrate the present invention further, these embodiments should be understood only be not used in for illustration of the present invention and limit the scope of the invention, after having read the present invention, the amendment of those skilled in the art to the various equivalent form of value of the present invention has all fallen within the application's claims limited range.
Embodiment 1
Material: material is one of them kind of oriental cherry " F. pendula Bean ", chooses raw stem segment with axillary bud then.
The method for tissue culture of the present embodiment F. pendula Bean comprises the following steps:
1, acquired for materials
At the noon of 3-5 month fair weather condition, intercept the healthy and strong disease-free spray upper position of F. pendula Bean as explant, remove blade and stay petiole.Optimality, pure, the accuracy of selected materials to be noted when gathering explant, must guard against and at will take, in order to avoid cause unnecessary loss.Growth selection is healthy and strong, without damage by disease and insect spring slightly branch section be material, to inoculate in time adopted material, prevent dehydration from wilting or pollute mildew and rot.
2, pretreatment
Repairing gathering the oriental cherry material come, removing unwanted part, cutting into 2-3cm segment, every section at least retains 1-2 axillalry bud, is then placed on by material in washing powder and soaks 15 minutes, repeatedly rinse 1h with flowing water.
3, sterilization
After pretreatment is carried out to material, proceed to superclean bench disinfection.Explant is cut into the long stem apex of 1-1.5cm or the stem section with an axillalry bud, with the ethanol postincubation 1min of 70%, then uses the HgCl of 0.1%
2disinfect 10min, constantly rock during process, make material fully reach sterilization effect, then use aseptic water washing 5 times.
4, Primary culture
Slightly prune through the stem apex base portion of sterilization, stem section two ends otch, be trimmed to simple bud stem apex or the stem-segment with single bud of 1-1.5cm.Vertical type is inoculated on Primary culture base and cultivates, and cultivation temperature is 25 DEG C, intensity of illumination 3000lx, light application time 12h/ days.
The formula of Primary culture base is: MS+6-BA0.5mg/L+NAA0.05mg/L+ sucrose 30g/L, and additional agar 6.5g/L, pH value is adjusted to 5.8.
5, Multiplying culture
By the plantlet in vitro grown through Primary culture 25d, intercept into the stem-segment with single bud of about 1.5cm, deduct the blade that can touch medium that bottom is larger, vertically be inoculated in proliferated culture medium and cultivate, cultivation temperature is 25 DEG C, intensity of illumination 3000lx, light application time 12h.
The formula of proliferated culture medium is: MS+6-BA0.3mg/L+NAA0.05mg/L+GA10mg/L+ sucrose 30g/L, and additional agar 6.5g/L, pH value is adjusted to 5.8.
6, culture of rootage
After Multiplying culture 20d, choose healthy and strong plantlet in vitro, cut off the callus that base portion expands, and the blade of medium can be touched, proceed to the induction that root media carries out root.Root media is:
1/2MS+NAA1.0mg/L+IBA0.5mg/L+ sucrose 20g/L, additional agar 6.5g/L, pH value is adjusted to 5.8.
Fig. 1 ~ Fig. 7 is the picture of different phase in the tissue culture procedures of the present embodiment F. pendula Bean, and through statistics, the differentiation-inducing rate of the present embodiment explant is 84%, and growth coefficient is 7.0, and rooting rate reaches 93%, and plantlet in vitro robust growth, internode obviously extends.
The optimization of embodiment 2 proliferation culture medium formula
Except proliferation culture medium formula is different from embodiment 1, all the other method steps are all identical with embodiment 1.
1, different 6-BA and NAA concentration impact that F. pendula Bean is bred
The formula of proliferated culture medium is: MS+6-BA+NAA+ sucrose 30g/L+ agar 6.5g/L, pH value is adjusted to 5.8.The concentration of 6-BA and NAA is see table 1.
Table 16-BA and NAA variable concentrations is on the impact of breeding in F. pendula Bean tissue cultures
As shown in table 1, the growth coefficient of all process is all lower, between 1.1 ~ 2.2.
2, minimal medium, 6-BA and NAA impact that F. pendula Bean is bred
The formula of proliferated culture medium is: minimal medium+6-BA+NAA+ sucrose 30g/L+ agar 6.5g/L, pH value is adjusted to 5.8.The concentration of 6-BA and NAA, the kind of minimal medium is see table 2.
By minimal medium 1/2MS, MS, 1/4MS, basic element of cell division BA (0.1,0.5,1.0), NAA (0.02,0.05,0.1), have chosen the L of three factor three levels
9(3
4) orthogonal design, result of the test is as table 2, and the growth coefficient of all process is still lower.
Table 2 minimal medium, 6-BA and NAA are on the impact of breeding in F. pendula Bean tissue cultures
Note: growth coefficient=propagation bud number/former inoculation bud number, the unified height of seedling being trimmed to 1-1.5cm of propagation bud number.
Above-mentioned two result of the tests find: in tested formula, growth coefficient is all lower, main cause is that internode does not extend, height of seedling is shorter, although the formula growth coefficient of MS+6-BA0.5mg/L+NAA0.1mg/L is 2.4, the formula growth coefficient of MS+6-BA0.5mg/L+NAA0.05mg/L is 2.2, but cultivation effect is still poor, and two kinds of formula Analysis of variances do not have significant difference.
3, hormonal readiness is adjusted
In order to solve the problem that internode does not extend, the basis of above-mentioned formula with the addition of the gibberellin GA that can internode be promoted profound, and suitable adjustment has been carried out to other hormone concentrations, test following formula (pH of medium is 5.8) respectively:
(1) MS+6-BA0.5mg/L+NAA0.05mg/L+ sucrose 30g/L+ agar 6.5g/L;
(2) MS+6-BA0.5mg/L+NAA0.05mg/L+GA5mg/L+ sucrose 30g/L+ agar 6.5g/L;
(3) MS+6-BA0.5mg/L+NAA0.05mg/L+GA10mg/L+ sucrose 30g/L+ agar 6.5g/L;
(4) MS+6-BA0.5mg/L+NAA0.05mg/L+GA15mg/L+ sucrose 30g/L+ agar 6.5g/L;
(5) MS+6-BA0.3mg/L+NAA0.05mg/L+ sucrose 30g/L+ agar 6.5g/L;
(6) MS+6-BA0.3mg/L+NAA0.05mg/L+GA5mg/L+ sucrose 30g/L+ agar 6.5g/L;
(7) MS+6-BA0.3mg/L+NAA0.05mg/L+GA10mg/L+ sucrose 30g/L+ agar 6.5g/L;
(8) MS+6-BA0.3mg/L+NAA0.05mg/L+GA15mg/L+ sucrose 30g/L+ agar 6.5g/L.
Table 3 hormon is on the impact of breeding in F. pendula Bean tissue cultures
Experimental result, as table 3, can find out that from the above results the formula that with the addition of gibberellin GA obviously can promote the elongation of F. pendula Bean internode, thus improves height of seedling, increase growth coefficient, and seedling growth is healthy and strong.It is not obvious that gibberellin adds the low facilitation effect of concentration, although also can promote internode elongation very well when concentration reaches 15mg/L, nursery stock is easily most advanced and sophisticated easily to turn yellow, and causes most advanced and sophisticated dead.And it is best with the effect of adding 10mg/L.Due to medium (3) and (7) difference in culture effect not obvious, consider cost-saving, Optimum formulae of selecting (7) to be Multiplying culture.
Claims (7)
1. a method for tissue culture for F. pendula Bean, comprising: the preparation of explant, Primary culture, Multiplying culture and culture of rootage; It is characterized in that, the medium of described Multiplying culture contains 6-BA0.2 ~ 0.5mg/L, NAA0.03 ~ 0.07mg/L and GA5 ~ 15mg/L.
2. the method for tissue culture of F. pendula Bean as claimed in claim 1; it is characterized in that, the formula of the medium of described Multiplying culture is: MS+6-BA0.2 ~ 0.5mg/L+NAA0.03 ~ 0.07mg/L+GA5 ~ 15mg/L+ sucrose 30 ~ 40g/L+ agar 5 ~ 8g/L.
3. the method for tissue culture of F. pendula Bean as claimed in claim 1 or 2, is characterized in that, in the medium of described Multiplying culture, the concentration of the concentration of 6-BA to be the concentration of 0.3 ~ 0.5mg/L, NAA be 0.05mg/L, GA is 10mg/L.
4. the method for tissue culture of F. pendula Bean as claimed in claim 1, it is characterized in that, the formula of the medium of described Primary culture is: MS+BA0.4 ~ 0.6mg/L+NAA0.03 ~ 0.07mg/L+ sucrose 30 ~ 40g/L+ agar 5 ~ 8g/L.
5. the method for tissue culture of F. pendula Bean as claimed in claim 4, is characterized in that, in the medium of described Primary culture, the concentration of 6-BA is the concentration of 0.5mg/L, NAA is 0.05mg/L.
6. the method for tissue culture of F. pendula Bean as claimed in claim 1, it is characterized in that, the method for described Multiplying culture comprises: Primary culture, after 20 ~ 30 days, gets the stem-segment with single bud of 1 ~ 2cm from plantlet in vitro, be vertically inoculated in medium and cultivate.
7. the method for tissue culture of F. pendula Bean as claimed in claim 1, it is characterized in that, described explant is stem segment with axillary bud or stem apex, takes from the middle and upper part of the slightly branch of raw spring then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510621194.1A CN105325291B (en) | 2015-09-25 | 2015-09-25 | The method for tissue culture of F. pendula Bean |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510621194.1A CN105325291B (en) | 2015-09-25 | 2015-09-25 | The method for tissue culture of F. pendula Bean |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105325291A true CN105325291A (en) | 2016-02-17 |
| CN105325291B CN105325291B (en) | 2017-08-11 |
Family
ID=55276282
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510621194.1A Active CN105325291B (en) | 2015-09-25 | 2015-09-25 | The method for tissue culture of F. pendula Bean |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105325291B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106538392A (en) * | 2016-12-08 | 2017-03-29 | 上海杉植物科技有限公司 | A kind of white oriental cherry tissue culture and rapid proliferation method |
| CN108271687A (en) * | 2017-01-06 | 2018-07-13 | 江苏省农业科学院宿迁农科所 | A kind of method for tissue culture of butterfly cherry |
| CN110476813A (en) * | 2019-09-03 | 2019-11-22 | 江苏农林职业技术学院 | A kind of oriental cherry rapid propagation in vitro method |
| CN110521595A (en) * | 2019-09-03 | 2019-12-03 | 江苏农林职业技术学院 | A method of promoting stem elongation in oriental cherry in vitro culture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101584299A (en) * | 2009-04-15 | 2009-11-25 | 河南省农业科学院 | A kind of red autumnal leaves oriental cherry tissue-culturing rapid propagation medium and tissue culture and rapid propagation method |
| CN102217534A (en) * | 2011-04-19 | 2011-10-19 | 南京林业大学 | Cerasus campanulata somatic cell embryogenesis method |
| CN105075784A (en) * | 2015-09-15 | 2015-11-25 | 安徽荣鸿农业开发股份有限公司 | Planting method and maintaining method of cerasus subhirtella var. pendula |
-
2015
- 2015-09-25 CN CN201510621194.1A patent/CN105325291B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101584299A (en) * | 2009-04-15 | 2009-11-25 | 河南省农业科学院 | A kind of red autumnal leaves oriental cherry tissue-culturing rapid propagation medium and tissue culture and rapid propagation method |
| CN102217534A (en) * | 2011-04-19 | 2011-10-19 | 南京林业大学 | Cerasus campanulata somatic cell embryogenesis method |
| CN105075784A (en) * | 2015-09-15 | 2015-11-25 | 安徽荣鸿农业开发股份有限公司 | Planting method and maintaining method of cerasus subhirtella var. pendula |
Non-Patent Citations (6)
| Title |
|---|
| 东京樱花组培快繁技术研究;朱育端;《林业勘察设计》;20121231(第1期);第158-160页，尤其是摘要和第159页最后一段。 * |
| 垂枝早樱‘红枝垂’组培快繁试验;荣冬青等;《林业科技开发》;20081231;第22卷(第5期);第72-75页，尤其是摘要。 * |
| 垂枝早樱‘红枝垂’组培快繁试验;荣冬青等;《林业科技开发》;20081231;第22卷(第5期);第72-75页，尤其是摘要以及第73页第1.1节，第1.3节。 * |
| 朱育端: "东京樱花组培快繁技术研究", 《林业勘察设计》 * |
| 王光萍等: "福建山樱花的组织培养及植株再生", 《南京林业大学报（自然科学版）》 * |
| 荣冬青等: "垂枝早樱‘红枝垂’组培快繁试验", 《林业科技开发》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106538392A (en) * | 2016-12-08 | 2017-03-29 | 上海杉植物科技有限公司 | A kind of white oriental cherry tissue culture and rapid proliferation method |
| CN106538392B (en) * | 2016-12-08 | 2019-04-19 | 上海杉一植物科技有限公司 | A kind of white oriental cherry tissue culture and rapid proliferation method |
| CN108271687A (en) * | 2017-01-06 | 2018-07-13 | 江苏省农业科学院宿迁农科所 | A kind of method for tissue culture of butterfly cherry |
| CN108271687B (en) * | 2017-01-06 | 2021-04-20 | 江苏省农业科学院宿迁农科所 | Tissue culture method of cerasus serrulata |
| CN110476813A (en) * | 2019-09-03 | 2019-11-22 | 江苏农林职业技术学院 | A kind of oriental cherry rapid propagation in vitro method |
| CN110521595A (en) * | 2019-09-03 | 2019-12-03 | 江苏农林职业技术学院 | A method of promoting stem elongation in oriental cherry in vitro culture |
| CN110476813B (en) * | 2019-09-03 | 2021-11-30 | 江苏农林职业技术学院 | In-vitro rapid propagation method for oriental cherry |
| CN110521595B (en) * | 2019-09-03 | 2021-11-30 | 江苏农林职业技术学院 | Method for promoting stem elongation in vitro culture of oriental cherry |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105325291B (en) | 2017-08-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103931492B (en) | The tissue culture fast seedling-cultivating method of apple rootstock M9 | |
| CN107047320B (en) | A kind of bigflower centranthera root method for tissue culture | |
| CN103931497B (en) | A kind of method improving dragon fruit plantlet in vitro planting percent | |
| CN103609224B (en) | Louisiana iris hybrid seed germination method | |
| CN103283598B (en) | Primary fir wood culture bud induction method | |
| CN102613076A (en) | Vegetative propagation method for butterfly orchid | |
| CN105325291B (en) | The method for tissue culture of F. pendula Bean | |
| CN107801634A (en) | A kind of establishing techniques of Radix Codonopsis vitro Regeneration System | |
| CN103766128A (en) | Asexual rapid propagation method for idesia seedlings | |
| CN106538392B (en) | A kind of white oriental cherry tissue culture and rapid proliferation method | |
| CN103283595A (en) | Primary culture method of stem tip tissue of strawberry | |
| CN103404438B (en) | Acer palmatum seed tissue culture method | |
| CN103651141B (en) | The method that Bo chrysanthemum batch production test tube seedling is the most numerous | |
| CN105379624A (en) | Tissue culture fast propagation method of Eucalyptus pellita | |
| CN103404437B (en) | Novel method for tissue culture rapid propagation of acer paimatum | |
| CN107494275A (en) | A kind of smilax tissue culture and rapid propagation method | |
| CN102613087B (en) | Method for culturing and breeding Correa carmen by using biological tissue | |
| CN103477988A (en) | Culture in vitro and rapid propagation method for syzygium grijsii | |
| CN106165648B (en) | A kind of cercis tissue culture culture medium and cultural method | |
| CN102907325B (en) | Method for utilizing culture technology to produce Solomon turmeric and red tulip seedlings | |
| CN102124953B (en) | Method for quickly breeding Litsea coreana test tube plantlets by spores | |
| CN109673514A (en) | A kind of method of south yampi type Chinese yam tissue-culturing quick-propagation | |
| CN110024688A (en) | A kind of method and its culture medium of caragana bud proliferation and plant regeneration | |
| CN104938340A (en) | Method for conducting rose tissue culturing rapid propagation on explant by using autumn and winter dormant bud | |
| CN109042335A (en) | A kind of culture medium and tissue culture mass production method of quick production grape virus-elimination seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20160217 Assignee: Shanghai Xuzhi Landscape Greening Co.,Ltd. Assignor: JIANGSU POLYTECHNIC College OF AGRICULTURE AND FORESTRY Contract record no.: X2023980047549 Denomination of invention: The tissue culture method of weeping cherry blossom Granted publication date: 20170811 License type: Common License Record date: 20231120 |
|
| EE01 | Entry into force of recordation of patent licensing contract |