CN105316231A - Constant temperature anaerobic fermentation device - Google Patents

Constant temperature anaerobic fermentation device Download PDF

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Publication number
CN105316231A
CN105316231A CN201510885850.9A CN201510885850A CN105316231A CN 105316231 A CN105316231 A CN 105316231A CN 201510885850 A CN201510885850 A CN 201510885850A CN 105316231 A CN105316231 A CN 105316231A
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Prior art keywords
fermentation
temperature
anaerobic fermentation
casing
constant
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CN201510885850.9A
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Chinese (zh)
Inventor
陈明仁
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Guangzhou Health Is For Bio Tech Ltd
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Guangzhou Health Is For Bio Tech Ltd
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Priority to CN201510885850.9A priority Critical patent/CN105316231A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/58Reaction vessels connected in series or in parallel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18External loop; Means for reintroduction of fermented biomass or liquid percolate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a constant temperature anaerobic fermentation device. The constant temperature anaerobic fermentation device comprises a box body as well as a temperature control device and a fermentation device which are arranged in the box body, wherein the fermentation device comprises at least two fermentation containers, and the temperature control device comprises a heating device and an airflow circulating device. By adopting the technical scheme of the invention, certain tanks of fermentative strains can be produced by regulating the number of the fermentation containers according to the actual demand in production, all the fermentation containers are separately operated and fermentation is united, the fermentative strains are taken as required in the production, and seals of the strains which are not used temporarily do not need to be opened, so that the quality of the fermentative strains can be guaranteed, and the sundry bacterium contamination possibility is reduced; and the fermentative strains are more convenient to take and use as the fermentative strains are dispersedly contained in corresponding small containers, so that strong application pertinence and easy operation are realized, and the problem of repeated strain taking and use after primary fermentation is solved.

Description

Constant-temperature anaerobic fermentation device
Technical field
The present invention relates to anaerobically fermenting field, particularly constant-temperature anaerobic fermentation device.
Background technology
Fermentation concept is known by common people, and the fermentation that people understand at first is a kind of " boiling " phenomenon, it be by the sugar in leach liquor degrade under anoxic conditions generation carbonic acid gas caused by.
Along with deepening continuously of using microbe research, the continuous progress of industrial technology, fermentation imparts different definition: in fact, fermentation is respiratory one, the chemical reaction that carries out of life entity and physiological change, that diversified biochemical reaction removes continuous local behavior according to the genetic information that life entity itself has, to obtain energy to the process of the activity of sustaining life.Tunning refers to that the metabolism that can regulate produced in the middle of reaction process or when reaction is reached home makes it to reach the material of balance.Therefore, the definition of modern fermentation is: by carrying out large-scale grown cultures to microorganism (or animal and plant cells), makes it chemical transformation and physiological change occur, thus produces and accumulate a large amount of people and to ferment the process of required meta-bolites.
Fermentable divides again different types, mainly divides the demand of oxygen according to the feature of fermenting and microorganism.Such as, solid state fermentation and liquid state fermentation can be divided into from what ferment in form; Batch fermentation can be divided into zymotechnique flow process, continuously ferment and fed-batch cultivation; Amino acid fermentation, organic acid fermentation, antibiotic fermentation, zymamsis, fermentation process for vitamin production etc. can be divided into from the difference of fermentation products therefrom; From participating in the need of oxygen being divided into aerobic fermentation, anaerobically fermenting and amphimicrobian fermentation.
Milk-acid bacteria is that a class can utilize carbohydrate fermentation to produce the general name of a class without gemma, gram-positive bacterium of lactic acid.Milk-acid bacteria can be divided into galactococcus and Bacterium lacticum again, and majority is amphimicrobian and strictly anaerobic bacterium, so from the need of providing the angular divisions lactobacillus-fermented of oxygen to belong to anaerobically fermenting type.So, the relevant device of lactobacillus-fermented, should be able to provide the temperature required for lactobacter growth, the requirements such as oxygen supply.
Existing fermentation equipment, in order to meet fermentable, generally universal equipment is made for the requirement of temperature, oxygen supply condition, i.e. condition adjustable type, not only anaerobically fermenting can be carried out but also aerobic fermentation can be carried out, the condition of different temperatures even requirement of extreme temperature conditions can also be met, but often expensive, diverse in function and operate inconvenience, large and the practical volume of floor space is little, and wants disposable taking after fermentation.
Summary of the invention
The object of this invention is to provide a kind of difficult problem that can solve one time fermentation and repeatedly take, use with strong points, and constant-temperature anaerobic fermentation device easy and simple to handle.
For achieving the above object, the present invention adopts following technical scheme, constant-temperature anaerobic fermentation device, comprises casing and the temperature regulating device that is arranged in casing and fermentation unit, fermentation unit comprises at least two fermenting containers, and temperature regulating device comprises heating unit and air current circulation.The setting of many groups fermenting container, can facilitate user to use fermentation by demand, and the independent fermentation operation of each group fermenting container, can take separately, solve the problem that one time fermentation is repeatedly taken, avoid the possibility of living contaminants, cut easy and simple to handle.
In some embodiments, be provided with liquid circulating apparatus in fermenting container, liquid circulating apparatus comprises recycle pump and circulating line, and recycle pump is placed on bottom fermenting container, and circulating line one end is communicated with recycle pump, and the other end is in fermenting container top.Circulating line is upward through inflection after the lid of fermenting container, again penetrates fermenting container lid and gets back in fermenting container, and forming fermented liquid derives from fermenting container through recycle pump, is returned the loop of fermenting container by conduit.
In some embodiments, heating unit is heating tube, and heating tube is fixed in casing.Heating tube volume is little, power large, and well heater primarily adopts cluster type tubular electrothermal element; Heat is echoed soon, temperature-controlled precision is high, and integrated heat efficiency is high.
In some embodiments, air current circulation comprises fan and the fixed support corresponding to fan.Fan and the fixed support corresponding to fan magnitude setting according to demand, fan drives the airflow circulation in casing, realizes the even of temperature in casing, accelerates heat trnasfer.
In some embodiments, temperature regulating device also comprises temperature measuring equipment, and temperature measuring equipment is connected with casing.Temperature in the real-time monitoring box body of temperature measuring equipment, the auxiliary constant temperature kept in casing.
In some embodiments, temperature measuring equipment comprises temperature probe and display unit, temperature probe end thereof contacts cabinets cavity, and one end connects display unit.By the temperature in temperature probe detection casing, the temperature in display unit display casing, the temperature in the real-time monitoring box body of user, ensures the carrying out of constant-temperatureanaerobic anaerobic fermentation.
In some embodiments, casing is provided with adiabator layer.Adiabator layer adopts lagging material to form, and reduces the heat losses in casing, contributes to the maintenance of temperature in casing.
In some embodiments, fermenting container capacity is 5L ~ 20L.Adopt relative small vessels to disperse splendid attire, take convenient.
Beneficial effect of the present invention is: after adopting technical solution of the present invention, can according to the fermented bacterium of actual demand amount in production by regulating the number of fermenting container to produce certain tank number, each fermenting container operates separately unified fermentation, fermented bacterium is taken as required in production, temporary transient no bacterial classification need not open the quality that sealing is conducive to ensureing fermented bacterium, reduces the possibility of living contaminants, simultaneously, owing to using corresponding small vessels to disperse splendid attire, take convenient.Reach use with strong points, easy and simple to handle, solve the problem that one time fermentation is repeatedly taken.
Accompanying drawing explanation
Fig. 1 is the structural representation of constant-temperature anaerobic fermentation device of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further detailed explanation.
As shown in Figure 1, constant-temperature anaerobic fermentation device, comprise casing 1 and the temperature regulating device 2 that is arranged in casing 1 and fermentation unit 3, fermentation unit 3 comprises two fermenting containers 31, and temperature regulating device 2 comprises heating unit 21, air current circulation 22 and temperature measuring equipment 23.
Liquid circulating apparatus is provided with in fermenting container 31, liquid circulating apparatus comprises recycle pump 32 and circulating line, recycle pump 32 is placed on bottom fermenting container 31, circulating line lower end connects recycle pump 32, circulating line upper end is upward through inflection after the lid of fermenting container 31, again penetrating fermenting container 31 lid gets back in fermenting container 31, and forming fermented liquid derives from fermenting container 31 through recycle pump 32, is returned the loop of fermenting container 31 by conduit.
Casing 1 internal fixtion installs a brandreth, and brandreth top is covered with fixed support plate, places fermenting container 31 above fixed support plate, fixed support plate is provided with the circular hole of diameter 3cm, interval between circular hole is about 15cm, is conducive to whole casing inner air convection, and homogeneous temperature distributes.
Heating unit 21 is heating tube, and by demand adjustment quantity, according to the present embodiment technical scheme demand, configure one group of heating tube, heating tube is fixed on fixed support plate.
Air current circulation 22 comprises fan and the fixed support corresponding to fan, and fan is fixed on brandreth by fixed support, and fan drives the airflow circulation in casing, realizes the even of temperature in casing, accelerates heat trnasfer.
Temperature measuring equipment 23 comprises a temperature probe 231 and display unit 232, temperature probe 231 is connected by wire with display unit 232, temperature probe 231 stretches into cabinets cavity from the fixing cavity of the side of casing, be fixed on wooden chest side, temperature detected by display unit 232 displays temperature probe 231, realizes the temperature variation of real-time monitoring box body 1 inside.
Casing 1 is provided with thermal insulation layer, and casing adopts wooden chest, and thermal insulation layer is positioned at wooden case interlayer, and thermal insulation layer adopts phenolic foam material.Phenolic foam material is the splendid fire-retardant heat-insulation material of heat-insulating property, reduces the heat losses in casing, contributes to the maintenance of temperature in casing.
Fermenting container 31 is the glass pot of 15L.Adopt relative small vessels to disperse splendid attire, take convenient.
After adopting technical solution of the present invention, can according to the fermented bacterium of actual demand amount in production by regulating the number of fermenting container to produce certain tank number, each fermenting container operates separately unified fermentation, fermented bacterium is taken as required in production, temporary transient no bacterial classification need not open the quality that sealing is conducive to ensureing fermented bacterium, reduces the possibility of living contaminants, simultaneously, owing to using corresponding small vessels to disperse splendid attire, take convenient.Reach use with strong points, easy and simple to handle, solve the problem that one time fermentation is repeatedly taken.
Above-described is only some embodiments of the present invention.For the person of ordinary skill of the art, without departing from the concept of the premise of the invention, can also make some distortion and improvement, these all belong to protection scope of the present invention.

Claims (8)

1. constant-temperature anaerobic fermentation device, it is characterized in that, the temperature regulating device (2) comprising casing (1) and be arranged in casing (1) and fermentation unit (3), described fermentation unit (3) comprises at least two fermenting containers (31), and described temperature regulating device (2) comprises heating unit (21) and air current circulation (22).
2. constant-temperature anaerobic fermentation device according to claim 1, it is characterized in that, described fermenting container is provided with liquid circulating apparatus in (31), described liquid circulating apparatus comprises recycle pump (32) and circulating line, described recycle pump (32) is placed on fermenting container (31) bottom, described circulating line one end is communicated with recycle pump (32), and the other end is in fermenting container (31) top.
3. constant-temperature anaerobic fermentation device according to claim 1, is characterized in that, described heating unit (21) is heating tube, and described heating tube is fixed in casing (1).
4. constant-temperature anaerobic fermentation device according to claim 1, is characterized in that, described air current circulation (22) comprises fan and the fixed support corresponding to fan.
5. constant-temperature anaerobic fermentation device according to claim 1, is characterized in that, described temperature regulating device (2) also comprises temperature measuring equipment (23), and described temperature measuring equipment (23) is connected with casing (1).
6. constant-temperature anaerobic fermentation device according to claim 5, it is characterized in that, described temperature measuring equipment (23) comprises temperature probe (231) and display unit (232), described temperature probe (231) end thereof contacts casing (1) inner chamber, one end connects display unit (232).
7. constant-temperature anaerobic fermentation device according to claim 1, is characterized in that, described casing (1) is provided with adiabator layer.
8. constant-temperature anaerobic fermentation device according to claim 1, is characterized in that, described fermenting container (31) capacity is 5L ~ 20L.
CN201510885850.9A 2015-12-04 2015-12-04 Constant temperature anaerobic fermentation device Pending CN105316231A (en)

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Application Number Priority Date Filing Date Title
CN201510885850.9A CN105316231A (en) 2015-12-04 2015-12-04 Constant temperature anaerobic fermentation device

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Application Number Priority Date Filing Date Title
CN201510885850.9A CN105316231A (en) 2015-12-04 2015-12-04 Constant temperature anaerobic fermentation device

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CN105316231A true CN105316231A (en) 2016-02-10

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201738381U (en) * 2010-06-24 2011-02-09 辽宁留德润滑油有限公司 Fire-fighting pool with aeration device
CN202131309U (en) * 2011-07-01 2012-02-01 余蓁茜 Constant temperature fermentation case
CN202754989U (en) * 2012-09-20 2013-02-27 王福军 Fermentation tank
CN203999625U (en) * 2014-07-06 2014-12-10 内蒙古民族大学 constant temperature fermentation box
CN204426645U (en) * 2014-12-10 2015-07-01 嘉善睿逸电子科技有限公司 Ferment at constant temperature device
CN205223249U (en) * 2015-12-04 2016-05-11 广州康代生物科技有限公司 Thermostatic anaerobic fermenter

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201738381U (en) * 2010-06-24 2011-02-09 辽宁留德润滑油有限公司 Fire-fighting pool with aeration device
CN202131309U (en) * 2011-07-01 2012-02-01 余蓁茜 Constant temperature fermentation case
CN202754989U (en) * 2012-09-20 2013-02-27 王福军 Fermentation tank
CN203999625U (en) * 2014-07-06 2014-12-10 内蒙古民族大学 constant temperature fermentation box
CN204426645U (en) * 2014-12-10 2015-07-01 嘉善睿逸电子科技有限公司 Ferment at constant temperature device
CN205223249U (en) * 2015-12-04 2016-05-11 广州康代生物科技有限公司 Thermostatic anaerobic fermenter

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Application publication date: 20160210