CN105273831B - A kind of broken wall of oil-containing micro-algae and the method for surfactant assisted extraction grease - Google Patents
A kind of broken wall of oil-containing micro-algae and the method for surfactant assisted extraction grease Download PDFInfo
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Abstract
The invention discloses the methods of a kind of broken wall of oil-containing micro-algae and surfactant assisted extraction grease, which is characterized in that described method includes following steps：(1) it is 7.5-14 the suspension of oil-containing micro-algae to be adjusted pH value, carries out hydro-thermal process；(2) after hydro-thermal process, enzyme digestion reaction is carried out in the presence of enzyme；(3) surfactant is added after enzyme digestion reaction to be reacted, organic solvent is then added and is extracted and isolates grease.The grease extraction efficiency of the method for the present invention is high, operating condition is mild, can effectively keep that the nutrition composition of microalgae, consumption of organic solvent are few, environmentally friendly, low energy consumption, and a variety of microalgaes such as micro- quasi- ball algae, chlorella, diatom, scenedesmus and single needle algae are can be suitably used for, it is applied widely.
Description
Technical field
The present invention relates to microalgae technical field of bioengineering, and in particular, to a kind of broken wall of oil-containing micro-algae and surface are lived
The method of property agent assisted extraction grease.
Background technology
Microalgae refers to that could distinguish the small algae colony of its form under the microscope, is most important first in biosphere
Grade productivity.The advantages that algae has biomass big, and with short production cycle, type is various, and oil content is high, strong environmental adaptability,
It is the very good material of biodiesel.In addition also contain the ingredients such as multivitamin, carrotene, protein, aliphatic acid in microalgae,
It is the source of active medicinal matter.
But microalgae grease all wraps up in the cell, in addition the bioactive substance of microalgae such as protein, and carotenoid is micro-
Polysaccharides etc. are most of to be all present in biological cell.Since microalgae cell is smaller and tool cell wall structure, most of types
Microalgae cell has a cellulosic cell wall, some kinds of microalgae cell wall be also largely difficult to digest decomposition it is other at
Point, cell wall thickness, quality is hard, is unfavorable for the extraction of grease, has been greatly reduced the bioavailability of microalgae product.Therefore micro-
The extraction of the broken wall and grease of algae is the key that grease and the other useful constituent utilized in microalgae.
The extraction of the grease of oil-containing micro-algae is always the key technology of biological diesel oil refining.Generally use hydro-thermal method at present.
Hydro-thermal method is typically to carry out breaking-wall cell, grease dissolution by high temperature and pressure under alkaline condition, and general temperature is 100-300
DEG C, high energy consumption, the molten slurry of gained oil-containing micro-algae carries out water-oil separating, such as CN100460513C, but the production due to obtaining by centrifugation
The oily molten slurry of microorganism is the emulsion containing microorganism slag, and it is uniform that oil phase is mixed with water conjunction, it is difficult to detach, oil is carried out by centrifuging
Water separating energy consumption is higher, grease extraction efficiency is relatively low.
Currently, the method for fracturing cell walls has polishing, multigelation method and ultrasonication method etc..Wherein polishing
It is cumbersome, and the easily intracellular original liquid component of collateral damage, clasmatosis degree is relatively low, can not achieve scale.Freeze repeatedly
Melt method and there is the problems such as can not achieve scale, operating cost is high, high energy consumption.Ultrasonication method can be destroyed intracellular other
Active component, operating cost are higher.
In addition, the extraction of microalgae grease is the key technology of the microalgae energy.Common Microalgae grease extraction method has super face
Boundary's carbon dioxide extraction method, soxhlet extraction methods, the methods of organic solvent extraction.Supercritical carbon dioxide extraction method operation is multiple
Miscellaneous, equipment is expensive, and severe reaction conditions are not easy to realize scale.There are organic molten for soxhlet extraction methods and organic solvent extraction
Agent usage amount is big, and toxicity is big, and pollution is big, the not high disadvantage of oil extracting rate.
CN102517146B discloses a kind of method of surfactant auxiliary aqueous extraction algae oil, is forming ultralow interface
Under conditions of tension and critical micro emulsion concentration, splits excusing from death is broken pot frustule and contain novel gemini surfactants
C10H21-18PO-2EO-SO4The saline solution of Na mixes, under conditions of 20-25 DEG C, with certain tachyphylaxis regular hour,
Obtained mixed liquor is centrifuged after reaction, upper layer is free lipid layer, and middle level is surfactant layer, lower layer
For water layer.The disadvantages of this method is to utilize ultrasonication, destroys intracellular other active components, and operating cost is higher, and should
Method is not directed to other algae, narrow application range only for pot algae is split.
Therefore, it is necessary to explore a kind of grease extraction efficiency is high, can effectively keep microalgae nutrition composition, operating condition it is mild,
The method for oil-containing micro-algae broken wall and the grease extraction that consumption of organic solvent is few, environmentally friendly, low energy consumption, applied widely.
Invention content
The purpose of the present invention is grease extraction efficiency is low in overcoming existing oil-containing micro-algae broken wall and grease to extract, fragile bad cell
Interior original liquid component, severe reaction conditions, consumption of organic solvent are big, environmental pollution is serious, high energy consumption or the shortcomings of narrow application range,
A kind of method that the broken wall and its grease extraction of new oil-containing micro-algae are provided.
The present inventor has found under study for action, and oil-containing micro-algae is carried out under alkaline condition and lower temperature condition
Hydro-thermal process and combine enzyme digestion reaction together broken wall be using a small amount of organic solvent then under the action of surfactant
The extraction of microalgae grease can be achieved.The grease extraction efficiency of this method is high, operating condition is mild, can effectively keep the nutrition group of microalgae
Point, consumption of organic solvent is few, environmentally friendly, low energy consumption, and it is more to can be suitably used for micro- quasi- ball algae, chlorella, diatom, scenedesmus and single needle algae etc.
Kind microalgae, it is applied widely.
Therefore, to achieve the goals above, the present invention provides a kind of broken walls of oil-containing micro-algae and surfactant to assist
The method for extracting grease, described method includes following steps：
(1) it is 7.5-14 the suspension of oil-containing micro-algae to be adjusted pH value, carries out hydro-thermal process；
(2) after hydro-thermal process, enzyme digestion reaction is carried out in the presence of enzyme；
(3) surfactant is added after enzyme digestion reaction to be reacted, organic solvent is then added and is extracted and is isolated
Grease.
Preferably, the condition of hydro-thermal process includes：Temperature is 50-120 DEG C, time 0.5-10h.
Preferably, the surfactant is Anionic-nonionic type surfactant and/or nonionic surfactant；More
Preferably, the Anionic-nonionic type surfactant is
The nonionic surfactant isIt is further preferred that the surface is lived
Property agent isWithAndWithMolar ratio be
1：4-6.
Preferably, the dosage of the suspension relative to the oil-containing micro-algae, the surfactant is 20-400mg/L.
Preferably, in terms of the dry weight of the oil-containing micro-algae, relative to oil-containing micro-algae described in 1g, the dosage of the organic solvent
For 1-30mL.
The method of the broken wall and surfactant assisted extraction grease of the oil-containing micro-algae of the present invention, due to using hydro-thermal process
With enzyme digestion reaction combined wall breakage, hydro-thermal process can be carried out under the conditions of relatively mild, high temperature and pressure is avoided, is conducive to microalgae
In each nutrition member biological property holding, reduce cost, improve shell-broken effect；Due to adding surfactant, have
The precipitation for helping microalgae grease greatly reduces the dosage of organic solvent, further reduces costs, and reduces to environment
Pollution；The grease extraction efficiency of the method for the present invention may be up to 80% or more, and grease extraction efficiency is high；The method of the present invention is easy to operate, and
A variety of microalgaes such as micro- quasi- ball algae, chlorella, diatom, scenedesmus and single needle algae are can be suitably used for, it is applied widely, it is easy to accomplish scale
Change.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Specific implementation mode
The specific implementation mode of the present invention is described in detail below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
The present invention provides the method for a kind of broken wall of oil-containing micro-algae and surfactant assisted extraction grease, this method packets
Include following steps：
(1) it is 7.5-14 the suspension of oil-containing micro-algae to be adjusted pH value, carries out hydro-thermal process；
(2) after hydro-thermal process, enzyme digestion reaction is carried out in the presence of enzyme；
(3) surfactant is added after enzyme digestion reaction to be reacted, organic solvent is then added and is extracted and is isolated
Grease.
In the present invention, oil-containing micro-algae refer to collecting from oil-containing micro-algae culture solution the oil-containing micro-algae obtained slurries or its
By dry algae powder.The method of the present invention is applicable to various oil-containing micro-algaes, i.e., does not limit particularly the type of oil-containing micro-algae
System, it is however preferred to have the oil-producing engineering microalgae of larger industrial utilization, for example, oil-containing micro-algae can be selected from micro- quasi- ball algae,
At least one of chlorella, diatom, single needle algae and scenedesmus.
In step (1) of the present invention, for the concentration of the suspension of oil-containing micro-algae, in terms of the dry weight of oil-containing micro-algae, oil-containing is micro-
Content of the algae in suspension is preferably 2-30 weight %, more preferably 15-30 weight %.
In step (1) of the present invention, it is 7.5-14, preferably 8.5-12 to adjust the suspension of oil-containing micro-algae to pH value, for
PH value condition can add alkali to be adjusted, and for alkali without particular/special requirement, alkali commonly used in the art may be used, for example, alkali can be with
It is one or more in hydroxide, carbonate, bicarbonate and phosphate.
In the present invention, pH value is measured by pH analyzers.
In the present invention, hydrothermal condition refers to keeping the condition of liquid at a certain temperature.The method of the present invention is due to using
Hydro-thermal process and enzyme digestion reaction joint carry out broken wall, and therefore, hydro-thermal process can carry out under the conditions of relatively mild, present invention step
Suddenly in (1), the condition of hydro-thermal process preferably includes：Temperature be 50-120 DEG C, more preferably 60-100 DEG C, time 0.5-10h,
More preferably 2-6h.It will be understood by those skilled in the art that hydro-thermal process preferably carries out under stiring.
100-300 DEG C of the temperature of the more conventional hydro-thermal process of temperature of the method for the present invention hydro-thermal process can greatly reduce energy
Consumption, and be conducive to the holding of the biological property of each nutrition member in microalgae.
In step (2) of the present invention, it can be various enzymes commonly used in the art, example to carry out enzyme used in enzyme digestion reaction
At least one of cellulase, pectase, protease, lysozyme and glusulase, preferably cellulase, fruit can be such as selected from
Glue enzyme, protease and lysozyme.These enzymes can be commercially available.
In step (2) of the present invention, for the dosage of enzyme, in terms of the dry weight of oil-containing micro-algae, the dosage of enzyme is preferably 50-
5000IU/g, more preferably 200-1200IU/g.When enzyme is the combination of several enzymes, the dosage of various enzymes is wanted without special
It asks, as long as the preferred above range of total amount.
In step (2) of the present invention, the condition of enzyme digestion reaction preferably includes：Temperature is 30-70 DEG C, more preferably 40-60 DEG C；
PH value is 3-7, more preferably 4-5.5；Time is 2-10h, more preferably until it can meet the condition of complete crushing microalgae cells
For 4-6h.It for pH value condition, can be adjusted with acid adding, for acid without particular/special requirement, acid commonly used in the art may be used,
Can be inorganic acid, or organic acid, for example, acid can be acetic acid, citric acid.
In step (3) of the present invention, surfactant is preferably Anionic-nonionic type surfactant and/or nonionic table
Face activating agent.Anionic-nonionic type surfactant is preferably
Nonionic surfactant is preferably
The present inventor has been surprisingly found that under study for action, when surfactant isWithCombination, andWithMolar ratio be 1：
When 4-6, the grease extraction efficiency of oil-containing micro-algae can be greatly improved, therefore, it is highly preferred that surfactant isWithCombination, andWithMolar ratio be 1：
4-6。
The principle of surfactant assisted extraction grease is：The ester group of grease molecules can be adsorbed on by interaction of hydrogen bond
In remnant tissue after microalgae wall breaking (such as polypeptide, glycolipid), surfactant molecule passes through hydrophilic head base and these remaining groups
It knits and generates stronger electrostatic interaction, competitive adsorption can be generated with grease molecules, grease molecules are replaced；Grease
Molecule has high interface energy and tends to be adsorbed in the cell tissue of relative hydrophobic, surface in water because of its hydrophobic property
Active agent molecule can form aggregation in aqueous solution and generate hydrophobic microcell, have strong increase-volume, dispersion energy to grease molecules
Power reduces oil water interfacial tension, grease molecules is made to be transferred into aqueous solution from cell tissue.
In the present invention, relative to the suspension of oil-containing micro-algae, the dosage of surfactant is preferably 20-400mg/L, more excellent
It is selected as 100-400mg/L.
In step (3) of the present invention, the condition that surfactant is reacted is added and preferably includes：Temperature is 20-80 DEG C, more
Preferably 40-60 DEG C, time 1-3h, more preferably 1.5-2.5h.It will be understood by those skilled in the art that in order to make table
Face activating agent preferably plays a role, when addition surfactant is reacted, it is preferred to use the modes such as oscillation, stirring make surface
Activating agent comes into full contact with algae solution, so that the grease molecules of oil-containing micro-algae preferably enter in aqueous solution, with more convenient organic molten
The extraction of agent.
In the present invention, organic solvent can be various organic solvents commonly used in the art, preferably chloroform, just oneself
Alkane, petroleum ether.
In the method for the present invention, due to adding surfactant, grease molecules are easier to be extracted by organic solvent, therefore,
The dosage for greatly reducing organic solvent, in terms of the dry weight of oil-containing micro-algae, relative to 1g oil-containing micro-algaes, the dosage of organic solvent is excellent
It is selected as 1-30mL, more preferably 2-10mL.
Moreover, the present inventor has found under study for action, the grease in microalgae is extracted in the prior art, using pole
Property the mode that shares of organic solvent and non-polar organic solvent surfactant is added using the method for the present invention, only use nonpolarity
Organic solvent extraction can reach polar organic solvent than in the prior art and non-polar organic solvent shares extraction preferably
Extraction effect, therefore, the method for further illustrating the present invention can greatly reduce consumption of organic solvent.
It will be understood by those skilled in the art that in order to make organic solvent preferably oil and grease extracting molecule, have in addition
When solvent is extracted, it is preferred to use the modes such as stirring, oscillation, ultrasonic wave make organic solvent and algae solution come into full contact with, contact
Time be generally 25-35min.
In the method for the present invention, be added after organic solvent extracted, grease molecules enter in organic solvent, isolate containing
The organic solvent of grease may be used various methods, such as centrifugation, stratification etc. commonly used in the art, preferably centrifuge.It is right
In centrifugation actual conditions without particular/special requirement, the normal condition of this field may be used, for example, can be at 4000-6000rpm
Centrifuge 5-15min.
After the isolated organic solvent containing grease, it will be understood by those skilled in the art that also needing to will be organic
Solvent removes, to obtain purer microalgae grease.The method of removal organic solvent can be thought for those skilled in the art
The various methods arrived, such as volatilize naturally, distill, be evaporated under reduced pressure etc., preferably it is evaporated under reduced pressure.
It will be understood by those skilled in the art that in order to be further reduced the dosage of organic solvent, it, can in actual production
With the organic solvent that will again be removed after organic solvent recycling several times in the organic solvent containing grease.
Embodiment
The present invention is further illustrated for embodiment below, but is not intended to limit the present invention.
In following embodiments and comparative example：
The weight of the weight of the grease of grease extraction efficiency=extraction/microalgae dry powder.
PH value is measured by pH analyzers (Mettler-Toledo Instrument (Shanghai) Co., Ltd., SG23).
Mixing speed is 30rpm.
Embodiment 1
The method that the present embodiment is used to illustrate the broken wall and surfactant assisted extraction grease of the oil-containing micro-algae of the present invention.
(1) the micro- quasi- ball algae dry powder of 5g is put into triangular flask, adds water to be configured to the suspension of 25 weight %, and adjust pH value
To 8.5,4h is stirred at 80 DEG C；
(2) pH value is adjusted to 5, and in terms of micro- quasi- ball algae dry powder, cellulase (Beijing letter is added according to the amount of 100IU/g
Profit is precious along Science and Technology Ltd., 2000IU/g, similarly hereinafter), pectase (letter profit in Beijing is precious along Science and Technology Ltd., 20000IU/g,
Similarly hereinafter), acid protease (letter profit in Beijing is precious along Science and Technology Ltd., 50000IU/g, similarly hereinafter) and lysozyme (Chinese medicines group
Reagent Co., Ltd, 20000IU/g, similarly hereinafter), react 5h at 50 DEG C；
(3) suspension relative to micro- quasi- ball algae is added according to the amount of 200mg/LWith WithMolar ratio be
1:5, it is stirred to react 2h at 50 DEG C, adds 20ml n-hexanes, 30min is stirred, centrifuges 10min at 5000rpm, take above
Organic solvent layer containing grease, then 100-200Pa are evaporated under reduced pressure at 68 DEG C, and constant weight drying obtains grease, grease carries
Rate is taken to be shown in Table 1.
Embodiment 2
The method that the present embodiment is used to illustrate the broken wall and surfactant assisted extraction grease of the oil-containing micro-algae of the present invention.
(1) 5g chlorella dry powder is put into triangular flask, adds water to be configured to the suspension of 15 weight %, and adjust pH value and arrive
10, stir 6h at 60 DEG C；
(2) pH value is adjusted to 4, and in terms of chlorella dry powder, cellulase, pectase, acid is added according to the amount of 200IU/g
Property protease and lysozyme, react 6h at 40 DEG C；
(3) it relative to the suspension of chlorella, is added according to the amount of 100mg/LWithAndWithMolar ratio be
1：4, it is stirred to react 2.5h at 40 DEG C, adds 10ml chloroforms, 25min is stirred, centrifuges 15min at 4000rpm, take
Organic solvent layer containing grease above, then 100-200Pa are evaporated under reduced pressure at 60 DEG C, and constant weight drying obtains grease, grease
Recovery rate be shown in Table 1.
Embodiment 3
The method that the present embodiment is used to illustrate the broken wall and surfactant assisted extraction grease of the oil-containing micro-algae of the present invention.
(1) 5g scenedesmus dry powder is put into triangular flask, adds water to be configured to the suspension of 30 weight %, and adjust pH value and arrive
12, stir 2h at 100 DEG C；
(2) pH value is adjusted to 5.5, and in terms of scenedesmus dry powder, cellulase, pectase, acid is added according to the amount of 300IU/g
Property protease and lysozyme, react 4h at 60 DEG C；
(3) it relative to the suspension of scenedesmus, is added according to the amount of 400mg/LWith WithMolar ratio be
1:6, it is stirred to react 1.5h at 60 DEG C, 50ml petroleum ethers (Beijing Chemical Plant, analysis are pure) is added, stirs 35min,
5min is centrifuged under 6000rpm, takes the organic solvent layer containing grease above, then 100-200Pa, is evaporated under reduced pressure at 50 DEG C, it is permanent
It dries again, obtains grease, the recovery rate of grease is shown in Table 1.
Embodiment 4
The method that the present embodiment is used to illustrate the broken wall and surfactant assisted extraction grease of the oil-containing micro-algae of the present invention.
The extraction of the broken wall and grease of oil-containing micro-algae is carried out according to the method for embodiment 1, unlike, in step (3), table
Face activating agent only withThe recovery rate of grease is shown in Table 1.
Embodiment 5
The method that the present embodiment is used to illustrate the broken wall and surfactant assisted extraction grease of the oil-containing micro-algae of the present invention.
The extraction of the broken wall and grease of oil-containing micro-algae is carried out according to the method for embodiment 1, unlike, in step (3), table
Face activating agent only withThe recovery rate of grease is shown in Table 1.
Embodiment 6
The method that the present embodiment is used to illustrate the broken wall and surfactant assisted extraction grease of the oil-containing micro-algae of the present invention.
The extraction of the broken wall and grease of oil-containing micro-algae is carried out according to the method for embodiment 1, unlike, in step (3), phase
For the suspension of micro- quasi- ball algae, surfactant is added according to the amount of 20mg/L.The recovery rate of grease is shown in Table 1.
Comparative example 1
The extraction of the broken wall and grease of oil-containing micro-algae is carried out according to the method for embodiment 1, unlike, in step (3), no
Surfactant is added, directly carries out the extraction of organic solvent.The recovery rate of grease is shown in Table 1.
Comparative example 2
The extraction of the broken wall and grease of oil-containing micro-algae is carried out according to the method for embodiment 1, unlike, save step (2).
The recovery rate of grease is shown in Table 1.
Table 1
| Serial number | The recovery rate of grease |
| Embodiment 1 | 94.3 |
| Embodiment 2 | 95.9 |
| Embodiment 3 | 96.7 |
| Embodiment 4 | 85.9 |
| Embodiment 5 | 86.1 |
| Embodiment 6 | 84.3 |
| Comparative example 1 | 63.2 |
| Comparative example 2 | 38.9 |
Embodiment 1-6 is compared with comparative example 1-2 as can be seen that the method for the present invention is anti-using hydro-thermal process and enzymolysis
Should the grease in oil-containing micro-algae, recovery rate be extracted using organic solvent by broken wall then under the action of surfactant together
It is high.
Embodiment 1 is compared with embodiment 4 and embodiment 5 respectively as can be seen that surfactant isWithCombination, andWithMolar ratio be
1：When 4-6, the grease extraction efficiency of oil-containing micro-algae can further be greatly improved；Embodiment 1 and embodiment 6 are compared can be with
Find out, relative to the suspension of oil-containing micro-algae, the dosage of surfactant is 100-400mg/L, can further increase oil-containing
The grease extraction efficiency of microalgae.
The method of the broken wall and surfactant assisted extraction grease of the oil-containing micro-algae of the present invention, due to using hydro-thermal process
With enzyme digestion reaction combined wall breakage, hydro-thermal process can be carried out under the conditions of relatively mild, high temperature and pressure is avoided, is conducive to microalgae
In each nutrition member biological property holding, reduce cost, improve shell-broken effect；Due to adding surfactant, have
The precipitation for helping microalgae grease greatly reduces the dosage of organic solvent, further reduces costs, and reduces to environment
Pollution；The grease extraction efficiency of the method for the present invention may be up to 80% or more, and grease extraction efficiency is high；The method of the present invention is easy to operate, and
A variety of microalgaes such as micro- quasi- ball algae, chlorella, diatom, scenedesmus and single needle algae are can be suitably used for, it is applied widely, it is easy to accomplish scale
Change.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail can carry out a variety of simple variants to technical scheme of the present invention within the scope of the technical concept of the present invention, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (13)
1. a kind of broken wall of oil-containing micro-algae and the method for surfactant assisted extraction grease, which is characterized in that the method packet
Include following steps：
(1) it is 7.5-14 the suspension of oil-containing micro-algae to be adjusted pH value, carries out hydro-thermal process；
(2) after hydro-thermal process, enzyme digestion reaction is carried out in the presence of enzyme；
(3) surfactant is added after enzyme digestion reaction to be reacted, organic solvent is then added and is extracted and isolated grease,
The surfactant is Anionic-nonionic type surfactant and/or nonionic surfactant, and the anion-is non-
Ionic surfactant isThe nonionic surfactant
ForRelative to the suspension of the oil-containing micro-algae, the dosage of the surfactant
For 20-400mg/L.
2. according to the method described in claim 1, wherein, in step (1), in terms of the dry weight of the oil-containing micro-algae, the oil-containing
Content of the microalgae in the suspension is 2-30 weight %.
3. according to the method described in claim 1, wherein, in step (1), the condition of hydro-thermal process includes：Temperature is 50-120
DEG C, time 0.5-10h.
4. according to the method described in claim 1, wherein, the enzyme be selected from cellulase, pectase, protease, lysozyme and
At least one of glusulase.
5. according to the method described in claim 4, wherein, in terms of the dry weight of the oil-containing micro-algae, the dosage of enzyme is 50-
5000IU/g。
6. according to the method described in claim 5, wherein, in terms of the dry weight of the oil-containing micro-algae, the dosage of enzyme is 200-
1200IU/g。
7. according to the method described in claim 1, wherein, in step (2), the condition of the enzyme digestion reaction includes：Temperature is 30-
70 DEG C, pH value 3-7, time 2-10h.
8. according to the method described in claim 7, wherein, temperature is 40-60 DEG C, pH value 4-5.5.
9. according to the method described in claim 1, wherein, the surfactant isAndMolar ratio
It is 1：4-6.
10. according to the method described in claim 1, wherein, in step (3), the condition packet that surfactant is reacted is added
It includes：Temperature is 20-80 DEG C, time 1-3h.
11. according to the method described in claim 1, wherein, the organic solvent is selected from chloroform, n-hexane, petroleum ether.
12. micro- relative to oil-containing described in 1g in terms of the dry weight of the oil-containing micro-algae according to the method described in claim 1, wherein
The dosage of algae, the organic solvent is 1-30mL.
13. according to the method described in claim 1, wherein, the oil-containing micro-algae is micro- quasi- ball algae, chlorella, diatom, single needle algae
At least one of with scenedesmus.
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| CN107916191A (en) * | 2017-12-29 | 2018-04-17 | 烟台智祺化学技术有限公司 | A kind of production method for not adding sulfur dioxide organic wine |
| CN108624403A (en) * | 2018-04-28 | 2018-10-09 | 武汉工程大学 | A kind of method of low temperature non-organic solvent extraction energy insect oils |
| CN109734264A (en) * | 2018-11-20 | 2019-05-10 | 江南大学 | A method of promoting the release of bloom blue algae content |
| CN114292702B (en) * | 2021-12-31 | 2023-06-16 | 河南省商业科学研究所有限责任公司 | Method for extracting peony essential oil by using surfactant aqueous solution and solid alkali |
| CN118028051B (en) * | 2024-04-11 | 2024-06-18 | 山东悦翔生物有限公司 | Method for extracting grease from microalgae |
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| CN101429467A (en) * | 2008-12-24 | 2009-05-13 | 青岛生物能源与过程研究所 | Method for simultaneously extracting lipid and protein from microalgae |
| CN102492544A (en) * | 2011-12-07 | 2012-06-13 | 湖北福星生物科技有限公司 | Method for preparing microalgal docosahexaenoic acid (DHA) oil by dry method |
| CN102517146A (en) * | 2011-12-22 | 2012-06-27 | 江南大学 | Auxiliary method for extracting algal oil by surfactant in water phase |
| CN103173273A (en) * | 2011-12-21 | 2013-06-26 | 新奥科技发展有限公司 | Microalgae grease extraction method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101429467A (en) * | 2008-12-24 | 2009-05-13 | 青岛生物能源与过程研究所 | Method for simultaneously extracting lipid and protein from microalgae |
| CN102492544A (en) * | 2011-12-07 | 2012-06-13 | 湖北福星生物科技有限公司 | Method for preparing microalgal docosahexaenoic acid (DHA) oil by dry method |
| CN103173273A (en) * | 2011-12-21 | 2013-06-26 | 新奥科技发展有限公司 | Microalgae grease extraction method |
| CN102517146A (en) * | 2011-12-22 | 2012-06-27 | 江南大学 | Auxiliary method for extracting algal oil by surfactant in water phase |
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