CN105267255A - Method of extracting Agrocybe aegerita polysaccharide from Agrocybe aegerita - Google Patents
Method of extracting Agrocybe aegerita polysaccharide from Agrocybe aegerita Download PDFInfo
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- CN105267255A CN105267255A CN201510735306.6A CN201510735306A CN105267255A CN 105267255 A CN105267255 A CN 105267255A CN 201510735306 A CN201510735306 A CN 201510735306A CN 105267255 A CN105267255 A CN 105267255A
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Abstract
The invention a method of extracting Agrocybe aegerita polysaccharide from Agrocybe aegerita, comprising following steps: (1), extracting Agrocybe aegerita at 30-40 DEG C for 1-3 h with hydrochloric acid solution having a mass fraction of 0.25-0.35% to obtain extract; (2), adding a base to the extract to adjust pH to 7.5-8.0, and removing insoluble substances by solid-liquid separation to obtain clarified aqueous solution A; (3), adding an acid to the aqueous solution A to adjust pH to 4.0-4.5, and removing insoluble substances by solid-liquid separation to obtain clarified aqueous solution B; (4), adding the aqueous solution B to macroporous resin columns, removing unadsorbed impurities by washing the macroporous resin column with water; washing macroporous resin with ethanol solution having a volume fraction of 15-20% to remove impurities higher in polarity; using ethanol solution having a volume fraction of 65-70% as a desorption solution to wash the macroporous resin, collecting the desorption solution after washing, and performing reduced pressure concentration and drying to obtain Agrocybe aegerita extract. The method has a reasonable process and high extraction efficiency, is simple to operate and is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of method extracting Methods of Polysaccharide From Agrocybe Chaxingu from agrocyb eaegerita, the present invention relates to biomedicine field.
Background technology
Agrocyb eaegerita, formal name used at school: agrocybeaegirit was the wild mushroom fungus of one of domestic high mountains and dense forests area, Lichuan, Jiangxi Camellia sinensis root and stem of certain plants portion growth originally.Agrocyb eaegerita contains 18 seed amino acids of needed by human body, and particularly containing the nutritional labeling such as 8 seed amino acids, glucosan, mycoprotein, carbohydrate that human body can not synthesize, its mushroom handle is tender and crisp tasty and refreshing, fragrant taste.Also have abundant vitamin B group and multi mineral prime element, if the elements such as ferrum, potassium, zinc, selenium are all higher than other mushroom, the traditional Chinese medical science thinks that this mushroom has the kidney invigorating, diuresis, controls the effects such as soreness of waist pain, eliminating dampness by diuresis, spleen invigorating, antidiarrheal, is the ultimate food of hypertension, cardiovascular and obesity patient.Its delicious flavour, tender and crisp good to eat, having again good health-care effect, is one of delicious rare edible fungi.
Agrocyb eaegerita is nutritious, and protein content is up to 19.55%.Have 18 seed amino acids in contained protein, what wherein content was the highest is that methionine accounts for 2.49%, is secondly glutamic acid, aspartic acid, isoleucine, glycine and alanine.Total amino acids content is 16.86%.8 seed amino acid content of needed by human are complete, and have the mineral element such as abundant vitamin B group and potassium, sodium, calcium, magnesium, ferrum, zinc.
Agrocyb eaegerita is containing significant antitumor polysaccharide, and have good antitumaous effect, people are called " Chinese refreshing mushroom ", " health food ", " anticancer point " it.
At present, mainly concentrate protein-based both at home and abroad to the report of agrocyb eaegerita, the extraction research carried out Methods of Polysaccharide From Agrocybe Chaxingu in agrocyb eaegerita is also less.
Summary of the invention
The technical problem that the present invention solves is, conveniently, from agrocyb eaegerita, extracts Methods of Polysaccharide From Agrocybe Chaxingu economically, improves the content of Methods of Polysaccharide From Agrocybe Chaxingu in agrocyb eaegerita extract.
Technical scheme of the present invention is, provides a kind of method extracting Methods of Polysaccharide From Agrocybe Chaxingu from agrocyb eaegerita, comprises the following steps:
(1) be that the hydrochloric acid solution of 0.25-0.35% extracts agrocyb eaegerita 1-3h at 30-40 DEG C with mass fraction, the mass ratio of hydrochloric acid solution and agrocyb eaegerita is 3-5:1, obtains extracting solution;
(2) described extracting solution is added alkali adjust ph to 7.5-8.0, then solid-liquid separation removing insoluble matter, obtain the water-soluble of clarification
Liquid A;
(3) in described water solution A, acid for adjusting pH value is added to 4.0-4.5, then solid-liquid separation removing insoluble matter, obtain clarification
Aqueous solution B;
(4) described aqueous solution B is joined in 100L macroporous adsorptive resins with the flow velocity of 50-100L/h, first remove not by the impurity adsorbed with the flow velocity of 100-200L/h washing macroporous adsorbent resin with water; By volume fraction be again the alcoholic solution of 15-20% with the flow velocity of 30-50L/h washing macroporous adsorbent resin except the higher impurity of depolarization; By volume fraction be again the alcoholic solution of 65-70% as stripping liquid with the flow velocity of 40-60L/h washing macroporous adsorbent resin, collect the stripping liquid after washing, through concentrating under reduced pressure, dry agrocyb eaegerita extract.
Further, in step (1), the mass fraction of described hydrochloric acid solution is 0.3%.
Further, described solid-liquid separation is filtration or centrifugal.
Further, described macroporous adsorbent resin is intermediate-polarity macroporous adsorption resin.
Further, described macroporous adsorbent resin is AB-8 macroporous adsorbent resin.
Further, described acid is acetic acid.
Further, described alkali is calcium hydroxide.
Further, in described step (4), the temperature of concentrating under reduced pressure is 60-65 DEG C.
Further, described drying is vacuum drying or spraying dry, and the moisture being dried to agrocyb eaegerita extract is no more than 5%.
Further, before described step (1), agrocyb eaegerita is crushed to below 0.5cm.
The usual employing of current similar substance be alcohol extraction, cost is high, removing impurity is precipitated for a long time with low temperature after concentrated, major defect is temperature requirement high (about 4 DEG C), time length (leaving standstill 12-48 hour), Impurity removal are incomplete, thus cause that the production cycle produces, cost is high, and active constituent content low, be difficult to industrialization.The advantage that the present invention gives prominence to is, employing aqueous hydrochloric acid solution extracts, cost is low, and do not need to concentrate, technique is simply coherent, alkali adjust ph is directly added to 7.5-8.0 after extracting solution solid-liquid separation, can precipitate rapidly under alkalescence at the impurity that acid condition is solvable in solution, add acetic acid adjust ph to 4.0-4.5, can precipitate rapidly under faintly acid at the impurity that weak basic condition is solvable in solution, do not need low temperature to leave standstill for a long time, then can dilute, be separated immediately by room temperature, greatly shorten the production cycle.Re-use and obtain macroporous adsorptive resins separation on acidic aqueous solution, the impurity of strong polarity cannot adsorb on resin, then adopts the washing with alcohol of 15-20% to remove the impurity of middle polarity, and Impurity removal is complete, and product effective ingredient is high.Extracting method conventional at present, in extract, the content of target product is generally 5%, and uses method of the present invention, and in extract, the content of Methods of Polysaccharide From Agrocybe Chaxingu can reach 40-60%.The product that the present invention prepares is mainly used in dietary supplement, nutrient and healthcare products.
Advantage of the present invention is:
1, in this method, the extraction continuity of Methods of Polysaccharide From Agrocybe Chaxingu is good, and technique is simple, is applicable to commercial introduction.
2, this method rational technology, active constituent content is high, extraction efficiency is high, simple to operate, be applicable to suitability for industrialized production.
3, the extract purity prepared by the present invention is stablized, and Methods of Polysaccharide From Agrocybe Chaxingu content is between 40-60%.
4, preparation method yield of the present invention is high; Solvent can recycling.
Detailed description of the invention
embodiment 1
Take dry agrocyb eaegerita 100kg and be crushed to below 0.5cm, drop in extraction pot, and add 0.3% hydrochloric acid solution 500L, open stirring; Open jacket steam when being heated to 40 DEG C, start timing, extract 3h, feed liquid crosses 400 order filter bags; After reflux, extract, feed liquid crosses 400 order filter bags, filtrate adding calcium hydroxide adjust ph to 7.5, centrifugalize; Centrifugal clear liquid adds acetic acid adjust ph to 5.0, centrifugalize; The centrifugal stillness of night joins in 100L macroporous adsorptive resins with the flow velocity of 80L/h, first removes not by the impurity adsorbed with the flow velocity of 120L/h washing macroporous adsorbent resin with water; By volume fraction be again 15% alcoholic solution with the flow velocity of 45L/h washing macroporous adsorbent resin except the higher impurity of depolarization; By volume fraction be again 65% alcoholic solution as stripping liquid with the flow velocity of 50L/h washing macroporous adsorbent resin, collect the stripping liquid after washing, through concentrating under reduced pressure, dry agrocyb eaegerita extract, Methods of Polysaccharide From Agrocybe Chaxingu content is 59.7%.
embodiment 2
Take dry agrocyb eaegerita 100kg and be crushed to below 0.5cm, drop in extraction pot, and add 0.25% hydrochloric acid solution 300L, open stirring; Open jacket steam when being heated to 30 DEG C, start timing, extract 2h, feed liquid crosses 400 order filter bags; After reflux, extract, feed liquid crosses 400 order filter bags, filtrate adding calcium hydroxide adjust ph to 8.0, centrifugalize; Centrifugal clear liquid adds acetic acid adjust ph to 4.5, centrifugalize; The centrifugal stillness of night joins in 100L macroporous adsorptive resins with the flow velocity of 50L/h, first removes not by the impurity adsorbed with the flow velocity of 100L/h washing macroporous adsorbent resin with water; By volume fraction be again 15% alcoholic solution with the flow velocity of 30L/h washing macroporous adsorbent resin except the higher impurity of depolarization; By volume fraction be again 70% alcoholic solution as stripping liquid with the flow velocity of 40L/h washing macroporous adsorbent resin, collect the stripping liquid after washing, through concentrating under reduced pressure, dry agrocyb eaegerita extract, Methods of Polysaccharide From Agrocybe Chaxingu content is 42.8%.
embodiment 3
Take dry agrocyb eaegerita 100kg and be crushed to below 0.5cm, drop in extraction pot, and add 0.35% hydrochloric acid solution 400L, open stirring; Open jacket steam when being heated to 35 DEG C, start timing, extract 1h, feed liquid crosses 400 order filter bags; After reflux, extract, feed liquid crosses 400 order filter bags, filtrate adding calcium hydroxide adjust ph to 7.8, centrifugalize; Centrifugal clear liquid adds acetic acid adjust ph to 4.8, centrifugalize; The centrifugal stillness of night joins in 100L macroporous adsorptive resins with the flow velocity of 100L/h, first removes not by the impurity adsorbed with the flow velocity of 200L/h washing macroporous adsorbent resin with water; By volume fraction be again 20% alcoholic solution with the flow velocity of 50L/h washing macroporous adsorbent resin except the higher impurity of depolarization; By volume fraction be again 65% alcoholic solution as stripping liquid with the flow velocity of 60L/h washing macroporous adsorbent resin, collect the stripping liquid after washing, through concentrating under reduced pressure, dry agrocyb eaegerita extract 312g, Methods of Polysaccharide From Agrocybe Chaxingu content is 54.5%.
Claims (10)
1. from agrocyb eaegerita, extract a method for Methods of Polysaccharide From Agrocybe Chaxingu, it is characterized in that, comprise the following steps:
(1) be that the hydrochloric acid solution of 0.25-0.35% extracts agrocyb eaegerita 1-3h at 30-40 DEG C with mass fraction, the mass ratio of hydrochloric acid solution and agrocyb eaegerita is 3-5:1, obtains extracting solution;
(2) described extracting solution is added alkali adjust ph to 7.5-8.0, then solid-liquid separation removing insoluble matter, obtain the water-soluble of clarification
Liquid A;
(3) in described water solution A, acid for adjusting pH value is added to 4.0-4.5, then solid-liquid separation removing insoluble matter, obtain clarification
Aqueous solution B;
(4) described aqueous solution B is joined in 100L macroporous adsorptive resins with the flow velocity of 50-100L/h, first remove not by the impurity adsorbed with the flow velocity of 100-200L/h washing macroporous adsorbent resin with water; By volume fraction be again the alcoholic solution of 15-20% with the flow velocity of 30-50L/h washing macroporous adsorbent resin except the higher impurity of depolarization; By volume fraction be again the alcoholic solution of 65-70% as stripping liquid with the flow velocity of 40-60L/h washing macroporous adsorbent resin, collect the stripping liquid after washing, through concentrating under reduced pressure, dry agrocyb eaegerita extract.
2. the method for claim 1, is characterized in that, in step (1), the mass fraction of described hydrochloric acid solution is 0.3%.
3. the method for claim 1, is characterized in that, described solid-liquid separation is filtration or centrifugal.
4. the method for claim 1, is characterized in that, described macroporous adsorbent resin is intermediate-polarity macroporous adsorption resin.
5. the method for claim 1, is characterized in that, described macroporous adsorbent resin is AB-8 macroporous adsorbent resin.
6. the method for claim 1, is characterized in that, described acid is acetic acid.
7. the method for claim 1, is characterized in that, described alkali is calcium hydroxide.
8. the method for claim 1, is characterized in that, in described step (4), the temperature of concentrating under reduced pressure is 60-65 DEG C.
9. the method for claim 1, is characterized in that, described drying is vacuum drying or spraying dry, is dried to
The moisture of agrocyb eaegerita extract is no more than 5%.
10. the method for claim 1, is characterized in that, before described step (1), is crushed to by agrocyb eaegerita
Below 5cm.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262505A (en) * | 2014-10-29 | 2015-01-07 | 安徽省科普产品工程研究中心有限责任公司 | Method for extracting edible mushroom polysaccharide |
| CN104940246A (en) * | 2015-07-17 | 2015-09-30 | 湖南杰萃生物技术有限公司 | Method for extracting coprinus comatus extractive from coprinus comatus |
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- 2015-11-03 CN CN201510735306.6A patent/CN105267255A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262505A (en) * | 2014-10-29 | 2015-01-07 | 安徽省科普产品工程研究中心有限责任公司 | Method for extracting edible mushroom polysaccharide |
| CN104940246A (en) * | 2015-07-17 | 2015-09-30 | 湖南杰萃生物技术有限公司 | Method for extracting coprinus comatus extractive from coprinus comatus |
Non-Patent Citations (1)
| Title |
|---|
| 孙志慧，: "《饮食宜忌与食物搭配》", 31 March 2014 * |
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Application publication date: 20160127 |