CN105254342A - Production method of Trichosanthes kirilowii Maxim and radices trichosanthis - Google Patents
Production method of Trichosanthes kirilowii Maxim and radices trichosanthis Download PDFInfo
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Abstract
The invention discloses a production method of Trichosanthes kirilowii Maxim and radices trichosanthis, and belongs to the field of traditional Chinese medicine production. According to the production method, nutrients are applied in growth process. The nutrients are prepared via following steps: a, 100g of crude nucleic acid is dissolved in a KOH solution, and an obtained mixture is subjected to stirring, filtering, neutralizing, concentration, concentration, drying, and smashing so as to obtain a finished product; or b, 6g of a nucleotide salt and a deoxynucleotide salt is smashed so as to obtain a finished product; or c, 4g of a nucleotide salt and a deoxynucleotide salt is smashed so as to obtain a finished product; or d, 2g of a nucleotide salt and a deoxynucleotide salt are mixed with 18g of starch so as to obtain a finished product; wherein the nucleotide salt is a mixture of four 5'-sodium nucleotide or potassium nucleotide, and the deoxynucleotide salt is a mixture of five 5'-sodium deoxynucleotide or potassium deoxynucleotide. In the step b, or c, or d, the amount of the nucleotide salt accounts for 88 to 96wt%, and the amount of the deoxynucleotide salt accounts for 4 to 12wt%. When the length of the cirrus of Trichosanthes kirilowii Maxim is about 30cm, a nutrient prepared via the step a or d is applied; at the early blooming period, a nutrient prepared via the step b is applied; and at the full-blossom period, a nutrient prepared via the step c is applied. The production method is simple; Trichosanthes kirilowii Maxim yield is increased by 20 to 30%; and radices trichosanthis yield is increased by 30%.
Description
The application is application number: 201310579990.4, the applying date: on November 19th, 2013, and denomination of invention is the divisional application of " purposes of the composition containing nucleosides hydrochlorate and deoxynucleoside hydrochlorate ".
Technical field
The present invention relates to a kind of production method of Chinese medicine, particularly the production method of a kind of snakegourd and Snakegourd Root.
Background technology
The growth of plant be unable to do without and supplements the nutrients, present plant husbandry, mainly by executing N,P,K fertilizers and organic fertilizer to promote crop growth, it is very large to use the quality and flavor impact of too much fertilizer on farm crop, is unfavorable for the development of green agriculture and food safety and environmental protection.Therefore, people have studied and nucleic acid related growth element, nutrient substance, fertilizer.In theory: nucleic acid is the basic substance forming life, and be the basis of substance metabolism and energy metabolism, the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form determine that the power of this life entity is prosperous to decline.Therefore, nucleic acid substances improves for the output increased in farm crop and even the production of whole vegitabilia and product quality irreplaceable effect; The nucleic acid small molecules such as nucleosides, Nucleotide, pyrimidine and purine are several nucleotides salt and deoxynucleoside hydrochlorate and cooperation thereof particularly, not only can the growth of effectively regulating plant, can be absorbed rapidly by plant as nutrition source again, strengthen plant root vigor, improve plant anti-adversity ability, thus improve crop output, improve product quality.Nucleic acid small molecules has enhancing improving activity of root system to plant, improves root to the absorption of phosphorus, potash fertilizer; Improve the activity of farm crop malic enzyme and succsinic acid enzyme, promote the assimilative capacity of plant, strengthen photosynthetic intensity, to plant-growth have adjustment and trophism (Wang Xi etc. the research [J] of the application and function mechanism of nucleic acid degradation product in Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).Chinese patent literature (publication number: 101186534) disclose a kind of preparation method containing nucleic acid degradation product foliage fertilizer, concrete steps are: 1., lixiviate nuclease, 2., dissolve Yeast Nucleic Acid (alkali lye hydrotropy), 3., the hydrolysis of nucleic acid, nucleosides must be contained, Nucleotide, purine, the multiple nucleic acids small molecules such as pyrimidine and a small amount of reducing sugar and few large, in, trace element, the method take Yeast Nucleic Acid as raw material, with enzymatic hydrolysis, there is production cost high, degrade not thorough, few containing deoxynucleotide composition, mixed system nucleotide salt yield is low, various nucleosides hydrochlorate and the mutual mixing ratio imbalance of deoxynucleotide salt face, very large on result of use impact.The patent No.: 201010514217.6, Authorization Notice No. CN102079665B, name is called: the Chinese patent of " nutrient substance of a kind of Promoting plant growth and preparation method thereof ", disclose a kind of nutrient substance of Promoting plant growth, this nutrient substance it primarily of nucleosides hydrochlorate, deoxynucleoside hydrochlorate and sugar or amino acid composition, its weight percent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 20-80%, sugar or amino acid 20-80%.This patent exists that composition activeconstituents is not morely saved, not easily standing storage and preparation method are simple not, and purposes is problem not widely.
Summary of the invention
Object of the present invention overcomes above-mentioned deficiency exactly, provides one to decrease activeconstituents in composition, and its effect still can meet or exceed the patent No. and be: the snakegourd of " 201010514217.6 " nutrient substance and the production method of Snakegourd Root.
The present invention is achieved in that
The production method of snakegourd and Snakegourd Root, nutrient substance is used in its process of growth, described nutrient substance is: the crude product nucleic acid 100 grams that a. will obtain from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liters of 6N, under 40 DEG C of conditions, stir 10 hours, obtain and mainly refer to contain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of 5 '-uridylic acid dipotassium, four kinds of 5 '-mixed nucleotides, also has deoxynucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylic acid (AMP) dipotassium, 5 '-deoxycytidylic acid(dCMP) dipotassium, 5 '-deoxyuridylic acid dipotassium, , the sylvite of 5 '-deoxythymidylic acid dipotassium, five kinds of 5 '-deoxidation mixed nucleotides and the solution of impurity, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume to 20%, dissolve near dry Nucleotide potassium and deoxynucleotide potassium by proper amount of clear water, its solution adds alcohol and separates out, and crystallization suction filtration is dry, obtains 88-93 grams of white core thuja acid sylvite and deoxynucleoside acid potassium salt and water soluble mixt impurity, gains are pulverized, crosses 80 mesh sieves, obtained nutrient substance,
Or:
B. nucleosides hydrochlorate and deoxynucleoside hydrochlorate 6 grams is pressed, the method that nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, obtained nutrient substance;
Or:
C. nucleosides hydrochlorate and deoxynucleoside hydrochlorate 4 grams is pressed, the method that nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, the nutrient substance made;
Or:
D. press nucleosides hydrochlorate and deoxynucleoside hydrochlorate 2 grams, starch 18 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, the method mixed with starch, the nutrient substance made;
Nucleosides hydrochlorate refers to: 5 '-adenylic acid (AMP) disodium or potassium, 5 '-Sodium guanylate or potassium, 5 '-cytidylic acid disodium or potassium, 5 '-uridine monophosphate disodium or potassium, the sodium salt of four kinds of 5 '-mixed nucleotides or sylvite;
Deoxynucleoside hydrochlorate refers to: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-pancreatic desoxyribonuclease acid disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium or potassium, 5 '-deoxythymidine acid disodium or potassium, the sodium salt of five kinds of 5 '-deoxidation mixed nucleotides or sylvite;
The application process of nutrient substance is: spray with after water dissolution;
Nutrient substance use opportunity and concentration is: snakegourd from cultivation after Second Year spray three times every year, first time at stem climing length 30 cm, with a or d nutrient substance 20 grams, be watered 20 liters, evenly spray on snakegourd rattan leaf; Second time, at the plant blossom initial stage, is used b nutrient substance, is watered 30 liters, evenly spray on one mu of snakegourd; Third time, at full-bloom stage, is used c nutrient substance, is watered 40 liters, evenly spray on one mu of snakegourd.
The production method of snakegourd and Snakegourd Root, in b or c or d, nucleosides hydrochlorate and deoxynucleoside hydrochlorate weight percent are: nucleosides hydrochlorate 88-96%, deoxynucleoside hydrochlorate 4-12%.
The production method of snakegourd and Snakegourd Root, snakegourd volume increase 20-30%.
The production method of snakegourd and Snakegourd Root, Snakegourd Root volume increase 30%.
The nutrient substance of Promoting plant growth, primarily of nucleosides hydrochlorate and deoxynucleoside hydrochlorate composition, not sugary or/and amino acid.
The nutrient substance of Promoting plant growth, is characterized in that: in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%.
The nutrient substance of Promoting plant growth, in nucleosides hydrochlorate and deoxynucleoside hydrochlorate, weight percent is: nucleosides hydrochlorate 88-96%, deoxynucleoside hydrochlorate 4-12%.
The nutrient substance of Promoting plant growth, it also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate.
The nutrient substance of Promoting plant growth, the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-80%, weighting agent 20-90%.
The nutrient substance of Promoting plant growth, is characterized in that: the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-30%, weighting agent 70-90%.
The nutrient substance of Promoting plant growth, weighting agent is: starch is or/and Mierocrystalline cellulose.
The nutrient substance of Promoting plant growth, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are sodium salt or sylvite.
Promote the nutrient substance of thing growth, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are sodium salt or sylvite.
The nutrient substance of Promoting plant growth, weighting agent is: one or more of W-Gum, cyclodextrin, Xylo-Mucine.
The nutrient substance of a kind of Promoting plant growth of the present invention also can be described as the composition containing nucleosides hydrochlorate and deoxynucleoside hydrochlorate: the preparation method of the composition containing nucleosides hydrochlorate and deoxynucleoside hydrochlorate, composition is primarily of nucleosides hydrochlorate and deoxynucleoside hydrochlorate composition, not sugary or/and amino acid, its preparation take nucleic acid as raw material:
1., the hydrolysis of nucleic acid: the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid according to nucleic acid, buck weight ratio being 1:20-30, alkali lye is 5.5-7N, under 30-45 DEG C of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate adjusts pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume is to moisture 10-25%;
3., crystallization with clear water dissolve dry after nucleosides hydrochlorate and deoxynucleoside hydrochlorate, its solution adds alcohol and separates out, and crystallization suction filtration is dry, obtains white core thuja acid salt and deoxynucleoside hydrochlorate;
4., by nucleosides hydrochlorate and deoxynucleoside hydrochlorate pulverize, sieve.
Described preparation method, in described nucleic acid hydrolysis process, alkali lye used is sodium hydroxide or potassium hydroxide solution.
Described preparation method, it is characterized in that, composition also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, the weighting agent adding pulverizing mixes with nucleosides hydrochlorate and deoxynucleoside hydrochlorate, the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-80%, weighting agent 20-90%.
The preparation method of the composition containing nucleosides hydrochlorate and deoxynucleoside hydrochlorate, composition is primarily of nucleosides hydrochlorate and deoxynucleoside hydrochlorate composition, not sugary or/and amino acid, its preparation: be by weight percentage: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%, get commercially available nucleosides hydrochlorate and deoxynucleoside hydrochlorate, mix, pulverize, sieve.
Described preparation method, composition also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, the weighting agent adding pulverizing mixes with nucleosides hydrochlorate and deoxynucleoside hydrochlorate, the weight percent of nucleosides hydrochlorate and deoxynucleoside hydrochlorate and weighting agent is: nucleosides hydrochlorate and deoxynucleoside hydrochlorate 10-80%, weighting agent 20-90%.
The purposes of the composition containing nucleosides hydrochlorate and deoxynucleoside hydrochlorate, said composition is primarily of nucleosides hydrochlorate and deoxynucleoside hydrochlorate composition, not sugary or/and amino acid, weight percent is: nucleosides hydrochlorate 85-99%, deoxynucleoside hydrochlorate 1-15%, said composition is applied to Chinese medicine, grain, oil plant, veterinary antibiotics, tobacco leaf, cotton, tealeaves, flowers, forest produce and can improve its Yield and quality, quality, enhancing resistance, uniformity coefficient, reduce nuisance content, cost-saving, increase income, synergy.
The purposes of the composition containing nucleosides hydrochlorate and deoxynucleoside hydrochlorate, said composition is primarily of nucleosides hydrochlorate and deoxynucleoside hydrochlorate composition, not sugary or/and amino acid, said composition application on plant as fertilizer or nutrient substance.
The purposes of the composition containing nucleosides hydrochlorate and deoxynucleoside hydrochlorate, its spy levy in: also containing weighting agent in composition: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, this composition containing weighting agent also can be applied on plant as fertilizer or nutrient substance.
The purposes of the composition containing nucleosides hydrochlorate and deoxynucleoside hydrochlorate, said composition is primarily of nucleosides hydrochlorate and deoxynucleoside hydrochlorate composition, and not sugary or/and amino acid, said composition can be applied on plant as plant-growth regulator.
The purposes of the composition containing nucleosides hydrochlorate and deoxynucleoside hydrochlorate, also containing weighting agent in composition: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate, this composition containing weighting agent also can be applied on plant as plant-growth regulator.
[0023] weighting agent of the present invention: starch refers to: green starch, yam starch, wheat starch, sweet potato starch, W-Gum, water caltrop starch, Lotus Root Starch, Chinese Water Chestnut Starch etc., preferred W-Gum.Dextrin refers to: white dextrin, yellow starch gum and Britain glue, cyclodextrin.Mierocrystalline cellulose refers to: methylcellulose gum, Vltra tears, Natvosol, carboxymethyl cellulose etc.
The preparation method of the nutrient substance of Promoting plant growth provided by the present invention, concrete steps are as follows: take nucleic acid as raw material: one is for raw material with commercially available nucleic acid, two is adopt the nucleic acid of dry method or wet production to be raw material from the mycelium after fermentation plant fermentation: 1., the hydrolysis of nucleic acid is the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid of 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 DEG C of condition, stir 10-14 hours, obtain nucleosides hydrochlorate and deoxynucleoside acid salt solution, filter, filtrate adjusts pH8-8.5, concentrating under reduced pressure;
2., decolouring, dry concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume is to moisture 10-25%;
3., crystallization clear water dissolves the nucleosides hydrochlorate after doing and deoxynucleoside hydrochlorate, its solution adds alcohol and separates out, crystallization suction filtration is dry, obtain white core thuja acid salt and deoxynucleoside hydrochlorate (from the mycelium after fermentation plant fermentation adopt the nucleic acid of dry method or wet production be raw material also containing water soluble mixt and impurity);
4., by nucleosides hydrochlorate and deoxynucleoside hydrochlorate pulverize, cross 50-150 mesh sieve, mix.
In nucleic acid hydrolysis process described in this preparation method, alkali lye used is sodium hydroxide or potassium hydroxide solution.
Commercially available nucleic acid is seldom impure after raw material hydrolysis.
In the present invention, nucleosides hydrochlorate mainly refers to: 5 '-adenylic acid (AMP) disodium or potassium, 5 '-Sodium guanylate or potassium, 5 '-cytidylic acid disodium or potassium, the sodium salt of 5 '-uridine monophosphate disodium or potassium four kinds of 5 '-mixed nucleotides or sylvite; Deoxynucleoside hydrochlorate refers to: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium,, the sodium salt of 5 '-deoxythymidine acid disodium or potassium five kinds of 5 '-deoxidation mixed nucleotides or sylvite.The source of nucleosides hydrochlorate and deoxynucleoside hydrochlorate is take nucleic acid as raw material, adopt the nucleic acid of dry method or wet production for raw material with the mycelium after fermentation plant fermentation, this kind of nucleic acid is a small amount of thymus nucleic acid (DNA) of main mixing with Yeast Nucleic Acid (RNA), makes nucleosides hydrochlorate and deoxynucleotide salt mixture with alkali lye hydrolysis.
Beneficial effect of the present invention:
Because the present invention is not sugary or/and amino acid, decrease composition activeconstituents, so more economize in raw materials and cost.
Although the present invention decreases activeconstituents, its effect does not still subtract and even produces better effect.
The present invention is not particularly containing glucose, glucose easily absorbs moisture deliquescence, nutrient substance containing glucose places the deliquescence that namely absorbs water more than a year under general condition of storage, there is product caking, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are hydrolyzed further, cause quality product to reduce even to lose efficacy, so, the invention solves the problem of the not easily standing storage of nutrient substance.
. because the present invention also comprises weighting agent: one or more of starch, dextrin, Mierocrystalline cellulose, polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate etc., adapt to the adding of weighting agent the relevant regulations being generally not less than 20 grams as the foliage fertilizer minimum package of merchandise sales; Filler starch, dextrin, Mierocrystalline cellulose etc. also possess certain viscosity, make nutrient substance be attached to blade face after spraying and the plant surface time longer, make its action effect better; Weighting agent polyvidone, Magnesium Stearate, calcium sulfate or magnesium sulfate etc. have dispersiveness and oilness, can extend the quality guaranteed period of nutrient substance.
Due to preparation method of the present invention: 1, the nucleic acid of dry method or wet production can be adopted to be raw material from byproduct (tankage) mycelium after fermentation plant (active sludge, petroleum fermentation etc. after Gourmet Powder Factory, brew-house, antibiotics plant, yeast factory, Amino Acid Factory, paper mill, sewage disposal) fermentation, its price of this nucleic acid is low, is easy to cost-saving; 2, can adopt the preparation method be hydrolyzed for raw material with commercially available nucleic acid, products pure degree is high, and the proportioning of Yeast Nucleic Acid and thymus nucleic acid can adjust, product suitability and better effects if; 3, also can directly with commercially available 5 '-adenylic acid (AMP) disodium or potassium, 5 '-Sodium guanylate or potassium, 5 '-cytidylic acid disodium or potassium, 5 '-uridine monophosphate disodium or potassium and 5 '-Desoxyadenosine acid disodium or potassium, 5 '-deoxidation bird adenylic acid (AMP) disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium, the sodium salt of 5 '-deoxythymidine acid disodium or potassium five kinds of 5 '-deoxidation mixed nucleotides or sylvite preparation, be therefore suitable for more flexible, technique is simpler.
Purposes of the present invention is: as the fertilizer particularly application on plant as foliage fertilizer or nutrient substance; Also can apply on plant as plant-growth regulator;
The present invention is applied to Chinese medicine, grain, oil plant, veterinary antibiotics, tobacco leaf, cotton, tealeaves, flowers, forest produce and can improve its Yield and quality, quality, enhancing resistance, uniformity coefficient, reduce nuisance content, cost-saving, increase income, synergy.
So product of the present invention: saving, environmental protection (nontoxic, harmless, noresidue, without any adverse side effect), volume increase of many uses, increase income, synergy, strengthen resistance, improve quality effective.
The present invention and No. 201010514217.6 patents, traditional product and method contrast tables 1
The present invention and No. 201010514217.6 patents, traditional product and method contrast tables 2
The present invention implements to spray agency part effect in table 3 to concrete crop
Note: content's index detection method is carried out with reference to national standard or industry standard, content is pressed dry product and is calculated.
Embodiment
Embodiment 1: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 2 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and obtain nutrient substance.
Embodiment 2: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 4 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized and obtain nutrient substance.
Embodiment 3: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 6 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and obtain nutrient substance.
Embodiment 4: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 2 grams, starch 18 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix, obtain nutrient substance with starch.
Embodiment 5: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 3 grams, W-Gum 22 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix, obtain nutrient substance with W-Gum.
Embodiment 6: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 4 grams, wheat starch 16 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix, obtain nutrient substance with wheat starch.
Embodiment 7: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 5 grams, white dextrin 20 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix, obtain nutrient substance with white dextrin.
Embodiment 8: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 6 grams, cyclodextrin 20 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, and mix, obtain nutrient substance with cyclodextrin.
Embodiment 9: get Nucleotide potassium and 1 gram, deoxynucleotide potassium, W-Gum 9 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient substance with W-Gum.
Embodiment 10: get Nucleotide potassium and 1.5 grams, deoxynucleotide potassium, methylcellulose gum 8.5 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient substance with methylcellulose gum.
Embodiment 11: get Nucleotide potassium and 2 grams, deoxynucleotide potassium, carboxymethyl cellulose etc. 8 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient substance with carboxymethyl cellulose etc.
Embodiment 12: get Nucleotide potassium and 2.5 grams, deoxynucleotide potassium, polyvidone 7.5 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient substance with polyvidone.
Embodiment 13: get Nucleotide potassium and 3 grams, deoxynucleotide potassium, Magnesium Stearate 7 grams, Nucleotide potassium and deoxynucleotide potassium are pulverized, and mix, obtain nutrient substance with Magnesium Stearate.
Embodiment 14: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 1.5 grams, W-Gum 4.5 grams, 4 grams, magnesium sulfate, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and magnesium sulfate are pulverized, and mix, obtain nutrient substance with W-Gum.
Embodiment 15: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 2 grams, Natvosol 4 grams, 4 grams, calcium sulfate, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and calcium sulfate are pulverized, and mix, obtain nutrient substance with Natvosol.
Embodiment 16: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 3 grams, W-Gum 4 grams, Magnesium Stearate 3 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and Magnesium Stearate are pulverized, and mix, obtain nutrient substance with W-Gum.
Execute example 17: get nucleosides hydrochlorate and deoxynucleoside hydrochlorate 7 grams, W-Gum 4 grams, yellow starch gum 8 grams, 4 grams, calcium sulfate, nucleosides hydrochlorate and deoxynucleoside hydrochlorate and calcium sulfate are pulverized, and mix, obtain nutrient substance with W-Gum, yellow starch gum.
Embodiment 18: by 100 grams, the crude product Yeast Nucleic Acid (RNA) obtained from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 DEG C of conditions, stir 12 hours, obtain and mainly refer to contain: 5 '-adenylic acid (AMP) disodium, 5 '-bird adenylic acid (AMP) disodium, 5 '-cytidylic acid disodium, the sodium salt of 5 '-uridine monophosphate disodium, four kinds of 5 '-mixed nucleotides (also has deoxynucleoside hydrochlorate: 5 '-Desoxyadenosine acid disodium, 5 '-deoxidation bird adenylic acid (AMP) disodium, 5 '-Deoxyribose cytidine acid disodium, 5 '-deoxyuridine acid disodium, , the sodium salt of 5 '-deoxythymidine acid disodium, five kinds of 5 '-deoxidation mixed nucleotides and the solution of impurity), filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume is to moisture 15%, dissolve near dry new-nucleo and Sodium Deoxyribomuleotide by proper amount of clear water, its solution adds alcohol and separates out, and crystallization suction filtration is dry, about 85-90 grams, gains are pulverized, are crossed 100 mesh sieves by white core thuja acid salt and deoxynucleoside hydrochlorate and water soluble mixt impurity.
Embodiment 19: by 100 grams, the crude product Yeast Nucleic Acid (RNA) obtained from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liters of 6N, under 40 DEG C of conditions, stir 10 hours, obtain and mainly refer to contain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of 5 '-uridylic acid dipotassium, four kinds of 5 '-mixed nucleotides, also has deoxynucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylic acid (AMP) dipotassium, 5 '-deoxycytidylic acid(dCMP) dipotassium, 5 '-deoxyuridylic acid dipotassium, , the sylvite of 5 '-deoxythymidylic acid dipotassium, five kinds of 5 '-deoxidation mixed nucleotides and impurity (amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume to 20%, dissolve near dry Nucleotide potassium and deoxynucleotide potassium by proper amount of clear water, its solution adds alcohol and separates out, and crystallization suction filtration is dry, about 88-93 grams of white core thuja acid sylvite and deoxynucleoside acid potassium salt and water soluble mixt impurity.Gains are pulverized, crosses 80 mesh sieves.
Embodiment 20: by commercially available nucleosides hydrochlorate 90 grams, deoxynucleoside hydrochlorate 10 grams, mixing, be dissolved in the potassium hydroxide solution of 2.5 liters of 6N, under 40 DEG C of conditions, stir 10 hours, must contain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of 5 '-uridylic acid dipotassium, four kinds of 5 '-mixed nucleotides, also has deoxynucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-dGMP dipotassium, 5 '-deoxycytidylic acid(dCMP) dipotassium, 5 '-deoxyuridylic acid dipotassium, , 5 '-deoxythymidylic acid dipotassium, the solution of the sylvite of five kinds of 5 '-deoxidation mixed nucleotides, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume to 20%, with the nearly dry Nucleotide potassium of proper amount of clear water dissolving and deoxynucleotide potassium, its solution adds alcohol and separates out, and crystallization suction filtration is dry, about obtains 95-98 grams of white core thuja acid sylvite and deoxynucleoside acid potassium salt.Gains are pulverized, crosses 120 mesh sieves.
Embodiment 21; Paddy rice: spray twice, first time is watered 30 liters with 20 grams of nutrient substances, about concentration 100ppm for initial bloom stage (heading about 10%), evenly sprays on one mu of paddy rice.Second time is full-bloom stage, after first time sprays, within 5-6 days, be watered 30 liters with 20 grams of nutrient substances, evenly sprays and gets final product on one mu of paddy rice (mu volume increase 77-130 kilograms, volume increase about 18%).
Embodiment 22: spray twice, first time also can initial bloom stage in the flower bud phase, is watered 30 liters, evenly sprays on one mu of cotton with 30 grams of nutrient substances.Second time full-bloom stage is watered 36 liters with 30 grams, and evenly spray on one mu of cotton can (mu increases production more than 9%).
Embodiment 23: rape: spray twice, first time is initial bloom stage, is watered 20 liters sprays on one mu of rape with 20 grams of nutrient substances.Second time is full-bloom stage, is watered 30 liters, sprays on one mu of rape with 20 grams, volume increase 20-25 kilogram.
Embodiment 24: tobacco: spray twice, each use 30 grams, is often watered 30 kilograms, about concentration 200ppm, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, the south north time is slightly different), first time sprays, 3-6 day in June carry out second time to spray, about concentration 150ppm, Quality advance grade, volume increase about 24%.
Embodiment 25: tealeaves: at growth germination period, spray first time, after often plucking, spray once, about concentration 180ppm.Volume increase about 2%.
Embodiment 26: capsicum: each once at initial bloom stage and full-bloom stage, concentration 200-300ppm, mu volume increase 43% ~ 85%.
Embodiment 27: strawberry: spray in the fruitlet phase, be watered 18 liters with 10 grams and spray, spraying 3 ~ 4 times after harvesting can increase production 44% in batches.
Embodiment 28: Chinese medicinal materials: the Chinese medicinal materials of different sites, using method differs, generally each once at initial bloom stage and full-bloom stage, concentration 100-260ppm.
Embodiment 29: mushroom: spray once in every 3 days from fruiting, concentration 100-150ppm, volume increase 55-87%, shortens collecting time 1/3rd.
Embodiment 30; Tree Peony flower: spray once in every 5 days from the petal phase of 10-20%, concentration 200-350ppm, the florescence can extend more than 7 days, flower is many and large.
Embodiment 31: Root of Manyflower Glorybower: February, sky sprayed once before results, often bag is watered 18 kilograms, sprays 1 mu of ground Root of Manyflower Glorybower.Volume increase 30% ~ 40%.
Embodiment 32: the red sage root: spray twice, each with 6 grams, every 3 grams are watered 18 kilograms, spray on one mu of red sage root, and the time that sprays carries out first time and sprays between June, and carry out second time between July and spray, mu increases production 120 kg.
Embodiment 33: the bark of eucommia: first time is seedling stage, in April, with 20 grams of nutrient substances, is watered 20 liters, evenly sprays in one mu of bark of eucommia; Second time is the treelet phase, and the annual 3-4 month and the 5-6 month respectively spray once; Third time is for becoming the tree phase: annual 4-5 month and respectively spraying once the 7-8 month.
Embodiment 34: golden cypress: first time is nursery stage, with 20 grams of nutrient substances, is watered 20 liters, evenly sprays on one mu of golden cypress seedling; Second time is setting date, and spray once; Third time is for the patent medicine phase: annual 4-5 month and respectively spraying once the 7-8 month.
Embodiment 35: peppermint: due to peppermint 1 year results several, the turnout of plant is large, need consume larger nutrient, and first time sprays as seedling growth stage, with 20 grams of nutrient substances, is watered 30 liters, evenly sprays on one mu of peppermint seedling; Second time sprays as branch animated period: fill a prescription by embodiment 2, convert 30 premium on currency, evenly spray on one mu of peppermint seedling; Pregnant flower bud flowering period besides: fill a prescription by embodiment 1, embodiment 2, embodiment 3 respectively in August, September, October, be watered 20,30,40 liters respectively, the growing state according to peppermint respectively sprays once at bud stage, initial bloom stage, full-bloom stage.According in Nanping, Fujian, the first experimental result in Anhui circle, peppermint oil offtake can be made to nearly double, simultaneously large to the alcohol content effect improving crude peppermint oil bu.
Embodiment 36: snakegourd: perennial herbaceous stem rattan wood, after cultivation, Second Year sprays three times every year, and first time, at stem climing length 30 cm, with 20 grams of nutrient substances, is watered 20 liters, evenly sprays on snakegourd rattan leaf; Second time, at the plant blossom initial stage, is filled a prescription by embodiment 3, is watered 30 liters, evenly spray on one mu of snakegourd.Third time, at full-bloom stage, fills a prescription by embodiment 2, is watered 40 liters, evenly spray on one mu of snakegourd.Snakegourd can increase production 20%-30%, and the root (Snakegourd Root) of snakegourd also can increase production about 30% simultaneously.
Embodiment 37: cape jasmine: spray once at initial bloom stage and full-bloom stage, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of cape jasmine; Respectively spray once again at fruit development period and fruit expanding period: fill a prescription by embodiment 2, convert 30 premium on currency, evenly spray on one mu of cape jasmine; Can shedding be reduced, increase yield, improve quality.
Embodiment 38: Japanese Honeysuckle; Spray twice, first time also can initial bloom stage in the flower bud phase, is watered 30 liters, evenly sprays on one mu of Japanese Honeysuckle with 30 grams of nutrient substances.Second time full-bloom stage is watered 36 liters with 30 grams and evenly sprays on one mu of Japanese Honeysuckle.
Embodiment 39: Flos Rosae Chinensis; Spray twice, first time also can initial bloom stage in the flower bud phase, is watered 30 liters, evenly sprays on one mu of Flos Rosae Chinensis with 30 grams of nutrient substances.Second time full-bloom stage is watered 36 liters with 30 grams and evenly sprays on one mu of Flos Rosae Chinensis.
Embodiment 40: Inula Flower; Spray twice, first time also can initial bloom stage in the flower bud phase, is watered 30 liters, evenly sprays on one mu of Inula Flower with 30 grams of nutrient substances.Second time full-bloom stage is watered 36 liters with 30 grams and evenly sprays on one mu of Inula Flower.
Embodiment 41: Semen Lini: spray once at initial bloom stage and full-bloom stage, with 20 grams of nutrient substances, be watered 30 liters, evenly spray in one mu of Semen Lini; Respectively spray once again at fruit development period and fruit expanding period: fill a prescription by embodiment 2, convert 30 premium on currency, evenly spray in one mu of Semen Lini; Can shedding be reduced, increase yield, improve quality.
Embodiment 42: tsaoko: spray once at initial bloom stage and full-bloom stage, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of tsaoko; Respectively spray once again at fruit development period and fruit expanding period: fill a prescription by embodiment 2, convert 30 premium on currency, evenly spray on one mu of tsaoko; Can shedding be reduced, increase yield, improve quality.
Embodiment 43: Semen Cassiae: spray once at initial bloom stage and full-bloom stage, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of Semen Cassiae; Respectively spray once again at fruit development period and fruit expanding period: fill a prescription by embodiment 2, convert 30 premium on currency, evenly spray on one mu of Semen Cassiae; Can shedding be reduced, increase yield, improve quality.
Embodiment 44: Siberian Cocklour Fruit: spray once at initial bloom stage and full-bloom stage, with 20 grams of nutrient substances, be watered 30 liters, evenly spray on one mu of Siberian Cocklour Fruit; Respectively spray once again at fruit development period and fruit expanding period: fill a prescription by embodiment 2, convert 30 premium on currency, evenly spray on one mu of Siberian Cocklour Fruit; Can shedding be reduced, increase yield, improve quality.
Embodiment 45: wax gourd: first time is seedling stage, in April, with 20 grams of nutrient substances, is watered 20 liters, evenly sprays on one mu of wax gourd; Second time is vegetative period, and spray once; Third time is the achievement phase: spray once.
Embodiment 46: Pseudobulbus Cremastrae Seu Pleiones: spray once in every 3 days from fruiting, concentration 100-150ppm, volume increase 55-87%, shortens collecting time 1/3rd.
Embodiment 47: Motherwort Herb: spray once in every 3 days after emerging, concentration 100-150ppm, volume increase 55-87%.
Claims (4)
1. the production method of snakegourd and Snakegourd Root, its spy levy in: in its process of growth, use nutrient substance, described nutrient substance is: the crude product nucleic acid 100 grams that a. will obtain from the mycelium of fermentation plant's byproduct by dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liters of 6N, under 40 DEG C of conditions, stir 10 hours, obtain and mainly refer to contain: 5 '-adenylic acid (AMP) dipotassium, 5 '-bird adenylic acid (AMP) dipotassium, 5 '-cytidylic acid dipotassium, the sylvite of 5 '-uridylic acid dipotassium, four kinds of 5 '-mixed nucleotides, also has deoxynucleoside hydrochlorate: 5 '-deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylic acid (AMP) dipotassium, 5 '-deoxycytidylic acid(dCMP) dipotassium, 5 '-deoxyuridylic acid dipotassium, , the sylvite of 5 '-deoxythymidylic acid dipotassium, five kinds of 5 '-deoxidation mixed nucleotides and the solution of impurity, filter, filtrate is neutralized to pH8-8.5, be evaporated to appropriate, concentrated solution is added activated carbon decolorizing, suction filtration it, filtrate reduced in volume to 20%, dissolve near dry Nucleotide potassium and deoxynucleotide potassium by proper amount of clear water, its solution adds alcohol and separates out, and crystallization suction filtration is dry, obtains 88-93 grams of white core thuja acid sylvite and deoxynucleoside acid potassium salt and water soluble mixt impurity, gains are pulverized, crosses 80 mesh sieves, obtained nutrient substance,
Or:
B. nucleosides hydrochlorate and deoxynucleoside hydrochlorate 6 grams is pressed, the method that nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, obtained nutrient substance;
Or:
C. nucleosides hydrochlorate and deoxynucleoside hydrochlorate 4 grams is pressed, the method that nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, the nutrient substance made;
Or:
D. press nucleosides hydrochlorate and deoxynucleoside hydrochlorate 2 grams, starch 18 grams, nucleosides hydrochlorate and deoxynucleoside hydrochlorate are pulverized, the method mixed with starch, the nutrient substance made;
Nucleosides hydrochlorate refers to: 5 '-adenylic acid (AMP) disodium or potassium, 5 '-Sodium guanylate or potassium, 5 '-cytidylic acid disodium or potassium, 5 '-uridine monophosphate disodium or potassium, the sodium salt of four kinds of 5 '-mixed nucleotides or sylvite;
Deoxynucleoside hydrochlorate refers to: 5 '-Desoxyadenosine acid disodium or potassium, 5 '-pancreatic desoxyribonuclease acid disodium or potassium, 5 '-Deoxyribose cytidine acid disodium or potassium, 5 '-deoxyuridine acid disodium or potassium, 5 '-deoxythymidine acid disodium or potassium, the sodium salt of five kinds of 5 '-deoxidation mixed nucleotides or sylvite;
The application process of nutrient substance is: spray with after water dissolution;
Nutrient substance use opportunity and concentration is: snakegourd from cultivation after Second Year spray three times every year, first time at stem climing length 30 cm, with a or d nutrient substance 20 grams, be watered 20 liters, evenly spray on snakegourd rattan leaf; Second time, at the plant blossom initial stage, is used b nutrient substance, is watered 30 liters, evenly spray on one mu of snakegourd; Third time, at full-bloom stage, is used c nutrient substance, is watered 40 liters, evenly spray on one mu of snakegourd.
2. the production method of snakegourd and Snakegourd Root according to claim 1, its spy levy in: in b or c or d, nucleosides hydrochlorate and deoxynucleoside hydrochlorate weight percent are: nucleosides hydrochlorate 88-96%, deoxynucleoside hydrochlorate 4-12%.
3. the production method of snakegourd and Snakegourd Root according to claim 1 or 2, its spy is levying in snakegourd volume increase 20-30%.
4. the production method of snakegourd and Snakegourd Root according to claim 1 or 2, its spy is levying in Snakegourd Root volume increase 30%.
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| CN106688570A (en) * | 2016-12-15 | 2017-05-24 | 渠县金穗农业科技有限公司 | Planting method of cynanchum otophyllum short in growth cycle and high in drug property and quality |
| CN111758733B (en) * | 2019-04-01 | 2022-04-19 | 龚辰辉 | Repairing agent for citrus canker |
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2013
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| CN105198584A (en) | 2015-12-30 |
| CN105198586A (en) | 2015-12-30 |
| CN103588533A (en) | 2014-02-19 |
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| CN105272530A (en) | 2016-01-27 |
| CN105272533A (en) | 2016-01-27 |
| CN105272517A (en) | 2016-01-27 |
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