CN105248456B - With the active Chinese medical extract of anti-rot Ditylenchus dipsaci and its application - Google Patents
With the active Chinese medical extract of anti-rot Ditylenchus dipsaci and its application Download PDFInfo
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- CN105248456B CN105248456B CN201510861332.3A CN201510861332A CN105248456B CN 105248456 B CN105248456 B CN 105248456B CN 201510861332 A CN201510861332 A CN 201510861332A CN 105248456 B CN105248456 B CN 105248456B
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- radix angelicae
- angelicae sinensis
- ditylenchus dipsaci
- chinese medical
- rot
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Abstract
The invention discloses one kind having the active Chinese medical extract of anti-rot Ditylenchus dipsaci, the present invention takes fresh Radix Angelicae Sinensis cauline leaf to spontaneously dry or after 60 DEG C of low temperature dryings, it crushes, sieving, obtains Radix Angelicae Sinensis drying cauline leaf, Radix Angelicae Sinensis is taken to dry cauline leaf, it is extracted with petroleum ether, ethyl acetate, n-butanol or water, obtains extract, concentrated, it is dry, obtain that there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci.The present invention is using discarded Radix Angelicae Sinensis cauline leaf as raw material; its bioactivity is screened by many experiments; it is preferred that obtaining that there is the good anti-rot active Chinese medical extract of Ditylenchus dipsaci; the cauline leaf that the present invention makes full use of Radix Angelicae Sinensis discarded; become Fei Weibao; the extract being prepared can be used for preparing environmentally protective, efficient, less toxic biological pesticide, has important environmental protection meaning and economic value, is worthy of promotion and application.
Description
Technical field
The present invention relates to a kind of Chinese medical extracts, and in particular to one kind is prepared using Radix Angelicae Sinensis cauline leaf as raw material with anti-
The active extract of rot stem nematodes.
Background technology
As the fast development of the big Chinese Medicine Industry in China and the continuous propulsion of health service industry and international market are to being derived from
The favor of natural resources product promotes Chinese medicine and the production scale of natural medicinal living resources raw material constantly to expand, resource warp
Ji industrial chain is extended and is expanded.At the same time, the Chinese medicine that flood tide is also produced in natural resources of Chinese medicinal materials industrialization process is discarded
Object.China's Chinese medicine and natural medicinal living resources produce area more than 2.40 × 106hm in recent years2, root yield is reachable
5.40×106Ton, and the histoorgans biomass such as discarded root system of plant, ground cauline leaf is up to 1.1 × 107-1.6 × 107Ton,
Cause the serious wasting of resources and environmental pollution.According to the preliminary statistics, the natural resources of Chinese medicinal materials industrialization process based on herbal pharmaceutical
In consume plant medicinal material about 7.0 × 10 every year5Ton, the up to millions of tons such as the discarded histoorgan of generation, waste residue, Chinese medicine are useless
The general water content of gurry is higher, and contains certain nutritional ingredient, easily corrupt, and serious pollution is easily caused to environment.Centering at present
The processing method of medicine waste：It stacks or buries first, being concentrated as waste, do not only take up a large amount of soils in this way, generate a large amount of
Harmful fermentation gas, and underground water easy to pollute；Second is that being burned after traditional Chinese medicine waste is dried, but a large amount of cigarettes are generated after burning
The emissions such as dirt, carbon dioxide cause the significant wastage of environmental pollution and resource.Therefore, in natural resources of Chinese medicinal materials industrialization process,
Should pay attention to traditional Chinese medicine waste lead to the problem of and its recycling, according to circular economy concept, it then follows economic benefit, Ecological Effect
The benefit development principle mutually unified with social benefit, improves comprehensive utilization of resources efficiency, extends resources economy industrial chain, promotes Chinese medicine
The sustainable health development of resource industries.
Rot stem nematodes (Ditylenchus destructor Thorne) belong to Nematoda, pad practises mesh, Ditylenchus, main
Be distributed in the U.S., Canada, Peru, Iran, Pakistan, Japan, New Zealand, South Africa and many European countries and I
The important inward plant quarantine nematode of state, is distributed mainly on the ground such as China Henan, Shandong, Hebei, Beijing.The nematode is mainly invaded
The several kinds of Chinese medicinal materials such as crops and Radix Angelicae Sinensis, peppermint, ginseng such as sweet potato, potato are contaminated, agricultural product and Chinese medicine is caused largely to subtract
Production, low quality, the nematodiasis is a kind of destructive disease.Therefore, effective and feasible control techniques are established, to promote
Into the raising of crops and Chinese medicine yield and quality.
The control method of rot stem nematodes includes mainly cultural control, physical control and chemical prevention etc. at present, however
The harm of Agro-chemicals control rot stem nematodes is still means important in current integrated control nematodiasis in actual production
One of, wherein organophosphorus insecticides are used for the chemical prevention of the nematode compared with multi items.China's sweet potato production district uses god more
Some medicaments such as agriculture pellet, phonamiphos, gram line pellet prevent rot stem nematodes, and certain effect is although achieved at prevention initial stage, but
Due to such medicament high poison, high residue, safety is low, Heavy environmental pollution；In addition, long-term a large amount of uses of these medicaments cause
Nematode generates resistance to pesticide, is allowed to be more difficult to wipe out and treat insect pests and plant diseases.Therefore, a kind of efficient, low toxicity, low-residual, low cost are developed
And bion nematicide easy to use become it is current there is an urgent need to.
Invention content
Goal of the invention：It is an object of the invention to make full use of Chinese medicine waste resource, become Fei Weibao, is original with Radix Angelicae Sinensis cauline leaf
Material, extraction obtains the extract with anti-rot Ditylenchus dipsaci purposes, provided by the invention to be carried by the Chinese medicine of raw material of Radix Angelicae Sinensis cauline leaf
Object is taken, environmentally protective, at low cost, anti-rot Ditylenchus dipsaci activity is strong, has important environmental protection meaning.
Technical solution：In order to achieve the goal above, the technical solution that the present invention takes is：
One kind having the active Chinese medical extract of anti-rot Ditylenchus dipsaci, it is prepared by the following method to obtain:It takes new
Fresh Radix Angelicae Sinensis cauline leaf spontaneously dries or after 60 DEG C of low temperature drying, crushes, sieving, obtains Radix Angelicae Sinensis drying cauline leaf, Radix Angelicae Sinensis is taken to dry stem
Leaf, with petroleum ether, ethyl acetate, n-butanol, ethyl alcohol or water extraction obtain extract, concentrate, dry, obtain having anti-rot
The active Chinese medical extract of Ditylenchus dipsaci.
Preferably, above-described that there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci, take Radix Angelicae Sinensis to dry stem
Leaf is added the petroleum ether extraction of Radix Angelicae Sinensis drying cauline leaf 6~15 times of volumes of weight, obtains ligroin extraction, concentrate, dry,
Obtain that there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci.
Preferably, above-described that there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci, extracting method to be back
Extraction method is flowed, ultrasonic extraction, continuous circumfluence extraction method, extraction time is 1~3 time, every time 0.5~2 hour.
It is provided by the invention that there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci to prepare anti-rot Ditylenchus dipsaci activity agriculture
Application in medicine preparation.
Anti-rot Ditylenchus dipsaci screening active ingredients experiment
One, experimental method
1, the preparation of Radix Angelicae Sinensis cauline leaf sample
Fresh Radix Angelicae Sinensis cauline leaf is spontaneously dried or after 60 DEG C of low temperature dryings, is crushed, sieving dries cauline leaf to get Radix Angelicae Sinensis
Sample.
2, the research of the anti-rot Ditylenchus dipsaci of Chinese medical extract
Radix Angelicae Sinensis is taken to dry cauline leaf sample, the alcohol reflux for being 70% with volumetric concentration extracts, and is concentrated to give extract, accurate to claim
It takes Radix Angelicae Sinensis to dry 70% ethanol extract 10mg of cauline leaf, is dissolved in DMSO sample mother liquor is made respectively.It is diluted with DMSO when experiment
Nematode contact toxicity measurement is carried out at the test solution of various concentration.It draws appropriate nematode suspension and is injected separately into 96 porocyte culture plates
Sample well in, nematode about 20~40 is contained per hole, supplement sterile water is to 300 μ L of total volume, and often processing sets 3 groups of repetitions, respectively
Be added respective concentration DMSO and sterile water as a contrast (CK).25 DEG C respectively culture 1,3,5, the laggard line worm death of 7d with
The statistics of survival condition.Calculation formula is as follows：
Nemic death rate (%)=(verge of death borer population/for trying nematode number) × 100%
3, the research of the anti-rot Ditylenchus dipsaci of Radix Angelicae Sinensis cauline leaf the different extracted parts
Suitable Radix Angelicae Sinensis cauline leaf drying sample is weighed, petroleum ether, ethyl acetate, n-butanol and water is respectively adopted, reflux carries
It after taking, is concentrated to dryness, is redissolved using DMSO, the test solution for being prepared into various concentration carries out nematode contact toxicity measurement.
Two, experimental result
1,500 μ g/mL, 250 μ g/mL, 125 μ g/mL, 62.5 μ g/mL, 31.25 μ g/mL are prepared using two-fold dilution's method,
The contact toxicity that the Radix Angelicae Sinensis stem-leaf extract test solution of 15.625 μ g/mL, 7.8125 μ g/mL carries out nematode measures, the results showed that, it is high
The Radix Angelicae Sinensis stem-leaf extract of concentration is stronger to the action of contace poison of nematode, when concentration is more than 31.25 μ g/mL, tags to nematode
It is little to act on substantially difference, wherein 250 μ g/mL Radix Angelicae Sinensis stem-leaf extracts are relatively strong to the lethality of nematode；In addition, with
The extension of time is co-cultured, the Radix Angelicae Sinensis stem-leaf extract of various concentration enhances the action of contace poison of nematode, when extract and nematode
It co-culturing 7 days, various concentration extracts basically reach 100% to the lethality of nematode, wherein 31.25 μ g/mL concentrations above
For extract when being co-cultured 5 days with nematode, the lethality of nematode can be more than 70% (as shown in Figure 1).
2, Radix Angelicae Sinensis cauline leaf is extracted using petroleum ether, ethyl acetate, n-butanol and water different solvents, will be carried
It after taking liquid low temperature to be concentrated to dryness, is redissolved using DMSO, obtains Radix Angelicae Sinensis cauline leaf the different extracted parts, and investigate the different extracted parts
Action of contace poison to nematode, the experimental results showed that, nonpolar extract part is stronger to the action of contace poison of nematode in Radix Angelicae Sinensis cauline leaf, especially
It is that the extract part of petroleum ether is most strong to the lethal effect of nematode, is co-cultured to the 3rd day with nematode, the lethality of nematode is reachable
To 58.2%, it is significantly higher than other test group lethalities.
Advantageous effect：It is provided by the invention that there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci to has the following advantages：
The present invention screens its bioactivity by many experiments, is preferably had using discarded Radix Angelicae Sinensis cauline leaf as raw material
The good anti-rot active Chinese medical extract of Ditylenchus dipsaci, the present invention become Fei Weibao, prepared by the cauline leaf for making full use of Radix Angelicae Sinensis discarded
Obtained extract can be used for preparing environmentally protective, efficient, less toxic biological pesticide, have important environmental protection meaning and
Economic value is worthy of promotion and application.
Description of the drawings
Fig. 1 is action of contace poison of the Chinese medical extract to rot stem nematodes of various concentration.
Specific implementation mode
With reference to specific embodiment, the present invention is furture elucidated, it should be understood that these embodiments be merely to illustrate the present invention and
It is not used in and limits the scope of the invention, after having read the present invention, various of equal value shapes of the those skilled in the art to the present invention
The modification of formula falls within the application range as defined in the appended claims.
Embodiment 1
One kind having the active Chinese medical extract of anti-rot Ditylenchus dipsaci, it is prepared by the following method to obtain:It takes new
It after fresh Radix Angelicae Sinensis cauline leaf spontaneously dries, crushes, sieving, obtains Radix Angelicae Sinensis drying cauline leaf, take Radix Angelicae Sinensis to dry cauline leaf, the oil measured with 8 times
Ether refluxing extraction 3 times 2 hours every time, obtains extract, concentrates, dry, obtains that there is the active Chinese medicine of anti-rot Ditylenchus dipsaci to carry
Take object.
Embodiment 2
One kind having the active Chinese medical extract of anti-rot Ditylenchus dipsaci, it is prepared by the following method to obtain:It takes new
Fresh Radix Angelicae Sinensis cauline leaf crushes after 60 DEG C of low temperature dryings, sieving, obtains Radix Angelicae Sinensis drying cauline leaf, takes Radix Angelicae Sinensis to dry cauline leaf, measured with 12 times
N-butanol continuous circumfluence extraction 2 times, 3 hours every time, obtain extract, concentrate, it is dry, obtain that there is anti-rot Ditylenchus dipsaci to live
The Chinese medical extract of property.
Embodiment 3
One kind having the active Chinese medical extract of anti-rot Ditylenchus dipsaci, it is prepared by the following method to obtain:It takes new
Fresh Radix Angelicae Sinensis cauline leaf crushes after 60 DEG C of low temperature dryings, sieving, obtains Radix Angelicae Sinensis drying cauline leaf, takes Radix Angelicae Sinensis to dry cauline leaf, measured with 10 times
Ethyl acetate, ultrasonic extraction 2 times 3 hours every time, obtains extract, concentrates, dry, obtains that there is anti-rot Ditylenchus dipsaci to live
The Chinese medical extract of property.
Using what the embodiment of the present invention 1~3 was prepared there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci, addition to help
The agricultural formulations such as missible oil, suspending agent, wettable powder, granula, aqua, mother liquor, female powder are made in agent.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (2)
1. there is application of the active Chinese medical extract of anti-rot Ditylenchus dipsaci in preparing anti-rot Ditylenchus dipsaci active agrochemical preparation,
Described there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci to be prepared by the following method to obtain:Radix Angelicae Sinensis is taken to dry stem
Leaf is added the petroleum ether extraction of Radix Angelicae Sinensis drying cauline leaf 6~15 times of volumes of weight, obtains ligroin extraction, concentrate, dry,
Obtain that there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci；
The method of the extraction is reflux extraction, ultrasonic extraction or continuous circumfluence extraction method, and extraction time is 1~3 time,
0.5~2 hour every time.
2. according to claim 1 there is the active Chinese medical extract of anti-rot Ditylenchus dipsaci to prepare anti-rot Ditylenchus dipsaci work
Application in property pesticidal preparations, which is characterized in that by Chinese medical extract and auxiliary agent be prepared into missible oil, suspending agent, wettable powder,
Granula, aqua, mother liquor or female powder agricultural formulations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510861332.3A CN105248456B (en) | 2015-11-30 | 2015-11-30 | With the active Chinese medical extract of anti-rot Ditylenchus dipsaci and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510861332.3A CN105248456B (en) | 2015-11-30 | 2015-11-30 | With the active Chinese medical extract of anti-rot Ditylenchus dipsaci and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105248456A CN105248456A (en) | 2016-01-20 |
| CN105248456B true CN105248456B (en) | 2018-07-13 |
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Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1081929C (en) * | 1998-10-28 | 2002-04-03 | 王卫 | Health preparation of fresh Chinese angelica and its preparation |
| CN100457124C (en) * | 2000-04-10 | 2009-02-04 | 宝生物工程株式会社 | Remedies |
| CN102058640B (en) * | 2010-12-14 | 2012-09-12 | 南京中医药大学 | Overground effective Chinese angelica parts with antibacterial activity as well as preparation method and application thereof |
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2015
- 2015-11-30 CN CN201510861332.3A patent/CN105248456B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| "银杏外种皮中银杏酚酸的提取工艺研究";田路飞;《东南大学硕士学位论文》;20150907;中文摘要 * |
| "银杏酚酸的分离鉴定及其抗菌活性研究";倪学文 等;《食品科学》;20040915;第59-63页 * |
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