CN105232600B - A kind of ginseng seed extract and its preparation method and application - Google Patents
A kind of ginseng seed extract and its preparation method and application Download PDFInfo
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- 235000008434 ginseng Nutrition 0.000 title claims abstract description 73
- 239000000284 extract Substances 0.000 title claims abstract description 52
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
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- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 14
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- 229930003944 flavone Natural products 0.000 claims description 6
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- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 6
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- 230000032683 aging Effects 0.000 description 4
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 4
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- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
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- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 2
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- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 2
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 2
- 235000005493 rutin Nutrition 0.000 description 2
- 229960004555 rutoside Drugs 0.000 description 2
- 208000013220 shortness of breath Diseases 0.000 description 2
- 235000010288 sodium nitrite Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 1
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- 240000004371 Panax ginseng Species 0.000 description 1
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 1
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- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to technical field of traditional Chinese medicines, a kind of ginseng seed extract and its preparation method and application is disclosed.The preparation method of ginseng seed extract of the present invention is by ginseng seed extracting in water, the concentration of gained ginseng seed extracting solution, loading is to macroporous absorbent resin, successively gradient concentration ethanol solution with deionized water, from low to high carries out gradient elution, the eluent of deionized water therein, the elution of 30v/v%-95v/v% concentration ethanol is collected respectively, removed under reduced pressure second alcohol and water obtains ginseng seed extract.The present invention is using ginseng seed as raw material, a kind of ginseng seed extract is obtained by specific process extraction, extracted ginseng seed extract has significant skin cell proliferation inhibitory activity, can be used in the preparation of skin cell proliferation inhibitor, has the potential advantages as resisting age of skin product.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicines, particularly relates to a kind of ginseng seed extract and preparation method thereof and answer
With.
Background technique
Skin aging is the skin aging phenomenon as caused by natural cause or non-natural factor.Skin histology day after people's birth
It reaches increasingly, function is gradually active, will start to degenerate when reaching certain age, and this degeneration is often unconsciously slow in people
Slow-motion row.The reason of causing skin aging has very much, the collagen as caused by light aging and extraneous factor and elastin
Reduction, cell run out of division number too early, enter programmed aging etc. in advance.
Cell Proliferation is that biology growing, development, breeding and the basis of heredity, cell are proliferated in a manner of dividing.
For Hayflick the study found that normal cell has certain service life, their proliferative capacity is also not unlimited, but has certain
Boundary, here it is famous Hayflick boundary is theoretical.Cell Proliferation is faster, and cell life is shorter.Slow down cell Proliferation
The life for exactly extending cell has delayed the process of aging, has reached anti-aging effect.The proliferation for slowing down cell makes skin thin
Born of the same parents temporarily in a dormant state, avoid that Skin Cell is allowed to run out of proliferative capacity too early, retain the capital of skin youth, be to slow down
One of rational method of skin ageing.
Ginseng seed, also known as ginseng seed are the fruit of araliaceae ginseng plant's ginseng Panax ginseng C.A.Mey.
It is real, it is the fruit stone for including a seed, is usually used in asthenia, giddy insomnia, shortness of breath uncomfortable in chest.Total saposins in ginseng seed contain
Amount is about 1-2%, and up to 40% unsaturated fatty acid, 30% protein are also contained in ginseng seed.In addition, in ginseng seed also
Containing 11 kinds of microelements, panaxan, amino acid, the ingredients such as polypeptide play great role to the intracorporal metabolic process of biology.
Up to the present, it does not find also to report the research of the inhibiting effect of skin cell proliferation ginseng seed.
Summary of the invention
In view of this, making the ginseng the purpose of the present invention is to provide a kind of ginseng seed extract and preparation method thereof
Seed extract has significant cell inhibitory effect activity, can be applied in cell inhibitory effect and preparation cell Proliferation presses down
Preparation has the potential advantages as resisting age of skin product.
In the field of Chinese medicines, ginseng seed is usually used in asthenia, giddy insomnia, the diseases such as shortness of breath uncomfortable in chest, and the present invention is by wound
Journal of Sex Research discovery is made, ginseng seed has preferably cell inhibitory effect activity, Neng Gouyong by the extract that ad hoc approach extracts
In the preparation of inhibition of cell proliferation.
The preparation method of ginseng seed extract of the present invention, comprising:
Step 1, ginseng seed extracting in water obtain ginseng seed extracting solution；
Step 2, the concentration of ginseng seed extracting solution, loading to macroporous absorbent resin successively use deionized water, ladder from low to high
It spends concentration ethanol solution and carries out gradient elution, collect deionized water therein, the elution of 30v/v%-95v/v% concentration ethanol respectively
Eluent, removed under reduced pressure second alcohol and water, obtain ginseng seed extract.
Ginseng seed extract of the present invention can be each single concentration ethanol in 30v/v%-95v/v% concentration and wash
De- eluent is also possible to their mixture.
Preferably, the preparation method includes:
Step 1, ginseng seed extracting in water obtain ginseng seed extracting solution；
Step 2, the concentration of ginseng seed extracting solution, loading to macroporous absorbent resin successively use deionized water, ladder from low to high
It spends concentration ethanol solution and carries out gradient elution, collect deionized water therein, 30v/v% (alternatively referred to as 30v/v% ethyl alcohol respectively
Elute position, it is similar below), 70v/v%, 95v/v% concentration ethanol elution eluent, removed under reduced pressure second alcohol and water, obtain
Ginseng seed extract.
Preferably, step 1 are as follows:
Ginseng seed crush, by 1g:10mL ratio be added deionized water, in water-bath 80-90 DEG C it is slightly boiled, extraction twice,
1 hour every time, centrifugation, supernatant liquid filtering collected all filtrates, obtained ginseng seed extracting solution.
Preferably, the gradient concentration ethanol solution of the step 2 from low to high is the ladder of 30v/v%-95v/v% concentration
Spend concentration ethanol solution.In certain specific embodiments of the invention, the gradient concentration ethyl alcohol of the step 2 from low to high is molten
Liquid is the gradient concentration ethanol solution being made of 30v/v% ethanol solution, 70v/v% ethanol solution and 95v/v% ethanol solution.
Preferably, the macroporous absorbent resin is HP-20 type macroporous absorbent resin.
Meanwhile the present invention also provides the ginseng seed extracts extracted by preparation method of the present invention, it is preferable that the people
Ginseng seed extract general flavone content is 0.14-1.91%.
Preparation method of the present invention is obtained into ginseng seed extract and carries out the active detection of cell inhibitory effect, as the result is shown institute
Skin fibroblasts proliferation can be significantly inhibited by stating ginseng seed extract.Accordingly, the present invention provides ginseng seed extracts to exist
Inhibit skin cell proliferation and the application in preparation skin cell proliferation inhibitor.Preferably, the Skin Cell is
Skin fibroblasts.
From the above technical scheme, the present invention obtains a kind of people by specific process extraction using ginseng seed as raw material
Join seed extract, extracted ginseng seed extract has significant skin cell proliferation inhibitory activity, it is thin to can be used in skin
In the preparation of born of the same parents' antiblastic, there are the potential advantages as resisting age of skin product.
Detailed description of the invention
Fig. 1 show ginseng seed extract to the inhibitory activity column diagram of proliferation of human dermal fibroblasts.
Specific embodiment
The embodiment of the invention discloses a kind of ginseng seed extracts and its preparation method and application.Those skilled in the art can
To use for reference present disclosure, it is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to this
It is it will be apparent that they are considered as being included in the present invention for the technical staff of field.Application of the invention by compared with
Good embodiment is described, related personnel obviously can not depart from the content of present invention, in spirit and scope to as described herein
Product and method are modified or appropriate changes and combinations, carrys out implementation and application the technology of the present invention.
For a further understanding of the present invention, below with reference to embodiment to a kind of ginseng seed extract provided by the invention and its
Preparation method and application are described in detail.
Embodiment 1: the preparation of ginseng seed extract of the present invention
The commercially available ginseng seed of 50g is crushed, by 1:10 (g/ml) addition deionized water, in water-bath 80-90 DEG C it is slightly boiled,
It extracts twice, 1 hour every time, centrifugation, supernatant liquid filtering collects all filtrates, is concentrated under reduced pressure, and is chromatographed with HP-20 macroreticular resin
Absorption, successively use deionized water, 30% ethanol elution, 70% ethanol elution and 95% ethanol elution, respectively collect deionized water,
30%, 70% and 95% ethanol eluate evaporating solvent under reduced pressure successively obtains ginseng seed extract A, ginseng seed extract B, ginseng
Seed extract C and ginseng seed extract D.
Embodiment 2: determination of total flavonoids in ginseng seed extract
Using 1 ginseng seed extract of embodiment as test object, while water in ginseng seed water extract and embodiment 1 is added
The ginseng seed extract for eluting position, according to Pharmacopoeia of People's Republic of China (2010 editions) method, using rutin as standard items, measurement
General flavone content in extract.Determination step are as follows: 1) standard curve making: precision measure reference substance solution 1ml, 2ml, 3ml,
4ml, 5ml, 6ml are set respectively in 25ml measuring bottle, and respectively plus 30% ethyl alcohol is to 6.0ml, add 5% sodium nitrite solution 1ml.It mixes, puts
Set 6 minutes, then plus 10% aluminum nitrate solution 1ml, shake up, place 6 minutes.Adding sodium hydroxide test solution 10ml, then plus 30% ethyl alcohol
It to scale, shakes up, places 15 minutes, using corresponding reagent as blank, with UV-VIS spectrophotometry, at the wavelength of 510nm
Absorbance is measured, using absorbance as ordinate, concentration is abscissa, draws standard curve.2) a certain amount of sample is taken to be placed in 25ml
It is other to measure absorbance in accordance with the law, simultaneously from " adding sodium nitrite solution 1ml " according to standard curve preparation step in volumetric flask
Test solution 3ml is taken, in addition to sodium hydroxide test solution is not added, remaining is ibid operated, and as blank, calculates general flavone in extract
Content (in terms of rutin).It the results are shown in Table 1.
General flavone content in 1 ginseng seed extract of table
| Sample ID | General flavone content % |
| Ginseng seed crude extract | 0.84 |
| Ginseng seed extract A | 0.36 |
| Ginseng seed extract B | 1.91 |
| Ginseng seed extract C | 0.88 |
| Ginseng seed extract D | 0.14 |
Embodiment 3: the active detection of cell inhibitory effect
According to embodiment 1 prepare ginseng seed extract, be completely dissolved in deionized water, be configured to respectively 0.8 μ g/ml~
100 μ g/ml solution to be measured, for measuring the inhibitory activity of cell proliferation.Determination step are as follows: first by human fibroblasts kind
Enter in 96 orifice plates (2000 cells/wells, 200 μ L)；Culture is moved back except culture medium for 72 hours, and 10 μ L of solution to be measured, culture 24 is added
After hour, the proliferation rate of cell is calculated by measurement cell relative populations using mtt assay.The testing principle of mtt assay is
Succinate dehydrogenase in living cells mitochondria can make exogenous MTT be reduced to the bluish violet crystallization first a ceremonial jade-ladle, used in libation of water-insoluble and deposit
In cell, and dead cell is without this function.Dimethyl sulfoxide can dissolve the first a ceremonial jade-ladle, used in libation in cell, with micropore spectrophotometric determination
550nm wavelength absorption value, can reflect living cells quantity indirectly.Within the scope of certain cell number, MTT crystallizes the amount to be formed and lives thin
Born of the same parents' number is directly proportional, the result is shown in Figure 1.
As shown in Figure 1, ginseng seed extract A-D of the present invention all has the inhibiting effect to skin fibroblasts proliferation,
Wherein ginseng seed extract B and ginseng seed extract C is in the concentration range of 0.8 μ of μ g/ml~100 g/ml, with the increasing of concentration
Greatly, inhibited proliferation enhances.
The above description of the embodiment is only used to help understand the method for the present invention and its core ideas.It should be pointed out that pair
For those skilled in the art, without departing from the principle of the present invention, the present invention can also be carried out
Some improvements and modifications, these improvements and modifications also fall within the scope of protection of the claims of the present invention.
Claims (7)
1. a kind of preparation method for the ginseng seed extract for inhibiting skin fibroblasts proliferation characterized by comprising
Step 1, ginseng seed extracting in water obtain ginseng seed extracting solution；
Step 2, the concentration of ginseng seed extracting solution, loading to macroporous absorbent resin are successively dense with deionized water, 30v/v%-95v/v%
The gradient concentration ethanol solution of degree carries out gradient elution, collects deionized water therein, 30v/v%, 70v/v%, 95v/v% respectively
The eluent of concentration ethanol elution, removed under reduced pressure second alcohol and water obtain ginseng seed extract.
2. preparation method according to claim 1, which is characterized in that step 1 are as follows:
Ginseng seed crushes, and deionized water is added by 1g:10mL ratio, in water-bath 80-90 DEG C it is slightly boiled, extraction twice, every time 1
Hour, centrifugation, supernatant liquid filtering collects all filtrates, obtains ginseng seed extracting solution.
3. preparation method according to claim 1, which is characterized in that the gradient concentration ethyl alcohol of the step 2 from low to high is molten
Liquid is the gradient concentration ethanol solution being made of 30v/v% ethanol solution, 70v/v% ethanol solution and 95v/v% ethanol solution.
4. preparation method according to claim 1, which is characterized in that the macroporous absorbent resin is HP-20 type macroporous absorption
Resin.
5. the ginseng seed of the inhibition skin fibroblasts proliferation of the preparation of preparation method described in claim 1-4 any one extracts
Object.
6. ginseng seed extract according to claim 5, which is characterized in that general flavone content 0.14-1.91%.
7. application of the ginseng seed extract described in claim 5 in preparation skin fibroblasts antiblastic.
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| CN116617137B (en) * | 2023-06-27 | 2023-10-20 | 甄萃(广东)创新技术有限公司 | Ginseng seed extract and preparation method thereof |
| CN118141862A (en) * | 2024-05-10 | 2024-06-07 | 通化东圣药品药材有限公司 | Ginseng composition, preparation method and detection method thereof |
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| CN1823933A (en) * | 2005-12-20 | 2006-08-30 | 北京天新园医药科技开发有限公司 | Ginseng aconite injection liquid and its preparation method |
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| CN1823933A (en) * | 2005-12-20 | 2006-08-30 | 北京天新园医药科技开发有限公司 | Ginseng aconite injection liquid and its preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 人参果化学成分研究;于明;《中国优秀硕士学位论文全文数据库》;20021231(第2期);第3-4页，尤其是第4页倒数第2段 |
| 人参皂苷Rg3对人成纤维细胞增殖和凋亡影响的实验研究;刘鹤松等;《中国实验诊断学》;20101130;第14卷(第11期);第1697-1700页 |
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