CN105232600A - Panax ginseng seed extract, preparing method of panax ginseng seed extract and application of panax ginseng seed extract - Google Patents
Panax ginseng seed extract, preparing method of panax ginseng seed extract and application of panax ginseng seed extract Download PDFInfo
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Abstract
The invention relates to the technical field of traditional Chinese medicine, and discloses a panax ginseng seed extract, a preparing method of the panax ginseng seed extract and application of the panax ginseng seed extract. The preparing method of the panax ginseng seed extract comprises the following steps of: performing extraction by adding water into panax ginseng seeds; concentrating the obtained panax ginseng seed extraction liquid; loading samples to macroporous adsorption resin; sequentially using deionized water and ethanol solutions with gradient concentrations from low to high for gradient elution; respectively collecting the deionized water and eluent eluted by ethanol with the concentration being 30v/v percent to 95 v/v percent; removing the ethanol and the water through pressure reduction; and obtaining the panax ginseng seed extract. The panax ginseng seeds are used as raw materials; the extraction is performed by a special method to obtain the panax ginseng seed extract; and the extracted panax ginseng seed extract has the obvious skin cell proliferation inhibition activity, can be used in the preparation of skin cell proliferation inhibitors, and has the potential advantages to become a skin anti-aging product.
Description
Technical field
The present invention relates to technical field of Chinese medicines, relate to a kind of Radix Ginseng seed extract and its preparation method and application specifically.
Background technology
Skin aging is the skin aging phenomenon caused by natural cause or non-natural factor.After people is born, skin histology is day by day flourishing, and function is enlivened gradually, and will start to degenerate when certain age of arrival, this degeneration is often slowly carried out unconsciously people.Cause the reason of skin aging to have a lot, the collagen protein caused as photoaging and extraneous factor and the minimizing of elastin, cell run out of division number of times too early, enter programmed aging etc. in advance.
Cell proliferation is the basis of biology growing, growth, breeding and heredity, and cell is bred in the mode of division.Hayflick studies discovery, and normal cell has certain life-span, and their multiplication capacity neither be unlimited, but have certain boundary, and famous Hayflick boundary that Here it is is theoretical.Cell proliferation is faster, and cell life is shorter.Slow down cell proliferation is exactly the life extending cell, has delayed aging process, has reached aging resistance effect.Slow down the propagation of cell, allow Skin Cell temporarily be in resting state, avoid allowing Skin Cell run out of multiplication capacity too early, retaining the capital of skin youth, is one of rational method slowing down skin ageing.
Radix Ginseng seed, is also called Fructus Ginseng, and be the fruit of araliaceae ginseng plant's Radix Ginseng PanaxginsengC.A.Mey., it is the pit including a seed, is usually used in body void weak, dizzy insomnia, sensation of oppression over the chest with shortness of breath.Total saponin content in Radix Ginseng seed is about 1-2%, also contains the unsaturated fatty acid up to 40%, the protein of 30% in Radix Ginseng seed.In addition, also containing 11 kinds of trace element in Radix Ginseng seed, ginseng polysaccharide, aminoacid, the compositions such as polypeptide, play great role to the metabolic process in organism.Up to the present, also do not find the inhibiting research report of Radix Ginseng seed for skin cell proliferation.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of Radix Ginseng seed extract and preparation method thereof, described Radix Ginseng seed extract is made to have significant cell inhibitory effect active, can be applied in cell inhibitory effect and prepare inhibition of cell proliferation, there are the potential advantages becoming resisting age of skin product.
At the field of Chinese medicines, it is weak that Radix Ginseng seed is usually used in body void, dizzy insomnia, the diseases such as sensation of oppression over the chest with shortness of breath, and the present invention finds through creative research, it is active that Radix Ginseng seed has cell inhibitory effect preferably through the extract that ad hoc approach extracts, and can be used in the preparation of inhibition of cell proliferation.
The preparation method of Radix Ginseng seed extract of the present invention, comprising:
Step 1, Radix Ginseng seed extracting in water, obtain Radix Ginseng seed extracting solution;
Step 2, Radix Ginseng seed extracting solution are concentrated, loading is to macroporous adsorbent resin, gradient elution is carried out successively with deionized water, gradient concentration alcoholic solution from low to high, collect the eluent of deionized water wherein, 30v/v%-95v/v% concentration ethanol eluting respectively, removed under reduced pressure second alcohol and water, obtains Radix Ginseng seed extract.
Radix Ginseng seed extract of the present invention can be the eluent from single concentration ethanol eluting each in 30v/v%-95v/v% concentration, also can be their mixture.
As preferably, described preparation method comprises:
Step 1, Radix Ginseng seed extracting in water, obtain Radix Ginseng seed extracting solution;
Step 2, Radix Ginseng seed extracting solution are concentrated, loading is to macroporous adsorbent resin, gradient elution is carried out successively with deionized water, gradient concentration alcoholic solution from low to high, collect deionized water wherein respectively, 30v/v% (also can be described as 30v/v% alcohol elution, below roughly the same), the eluent of 70v/v%, 95v/v% concentration ethanol eluting, removed under reduced pressure second alcohol and water, obtains Radix Ginseng seed extract.
As preferably, step 1 is:
Radix Ginseng seed is pulverized, and adds deionized water in 1g:10mL ratio, and in water-bath, 80-90 DEG C micro-ly boils, and extract twice, each 1 hour, centrifugal, supernatant liquid filtering, collects all filtrate, obtains Radix Ginseng seed extracting solution.
As preferably, described step 2 gradient concentration alcoholic solution is from low to high the gradient concentration alcoholic solution of 30v/v%-95v/v% concentration.In detailed description of the invention more of the present invention, described step 2 gradient concentration alcoholic solution is from low to high the gradient concentration alcoholic solution be made up of 30v/v% alcoholic solution, 70v/v% alcoholic solution and 95v/v% alcoholic solution.
As preferably, described macroporous adsorbent resin is HP-20 type macroporous adsorbent resin.
Meanwhile, the present invention also provides the Radix Ginseng extracted by preparation method of the present invention seed extract, and preferably, described Radix Ginseng seed extract general flavone content is 0.14-1.91%.
Preparation method of the present invention is obtained the detection that Radix Ginseng seed extract carries out cell inhibitory effect activity, and result shows described Radix Ginseng seed extract and can significantly suppress skin flbroblast to be bred.Accordingly, the invention provides Radix Ginseng seed extract and suppress skin cell proliferation and the application in preparation skin cell proliferation inhibitor.As preferably, described Skin Cell is skin flbroblast.
From above technical scheme, the present invention with Radix Ginseng seed for raw material, extract through specific process and obtain a kind of Radix Ginseng seed extract, the Radix Ginseng seed extract extracted has significant skin cell proliferation inhibit activities, can be used in, in the preparation of skin cell proliferation inhibitor, there are the potential advantages becoming resisting age of skin product.
Accompanying drawing explanation
Figure 1 shows that the inhibit activities bar diagram of Radix Ginseng seed extract to proliferation of human dermal fibroblasts.
Detailed description of the invention
The embodiment of the invention discloses a kind of Radix Ginseng seed extract and its preparation method and application.Those skilled in the art can use for reference present disclosure, and suitable improving technique parameter realizes.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are all deemed to be included in the present invention.Application of the present invention is described by preferred embodiment, related personnel obviously can not depart from content of the present invention, spirit and scope product as herein described and method are changed or suitably change with combination, realize and apply the technology of the present invention.
In order to understand the present invention further, below in conjunction with embodiment, a kind of Radix Ginseng seed extract provided by the invention and its preparation method and application is described in detail.
Embodiment 1: the preparation of Radix Ginseng seed extract of the present invention
Radix Ginseng seed commercially available for 50g is pulverized, deionized water is added by 1:10 (g/ml), in water-bath, 80-90 DEG C micro-ly boils, extract twice, each 1 hour, centrifugal, supernatant liquid filtering, collect all filtrate, concentrating under reduced pressure, with HP-20 macroporous resin chromatographic adsorption, use deionized water, 30% ethanol elution, 70% ethanol elution and 95% ethanol elution successively, collect respectively deionized water, 30%, 70% and 95% ethanol elution removes solvent under reduced pressure and obtains Radix Ginseng seed extract A, Radix Ginseng seed extract B, Radix Ginseng seed extract C and Radix Ginseng seed extract D successively.
Embodiment 2: determination of total flavonoids in Radix Ginseng seed extract
With embodiment 1 Radix Ginseng seed extract for detected object, add the Radix Ginseng seed extract at water elution position in Radix Ginseng seed water extract and embodiment 1 simultaneously, according to Pharmacopoeia of People's Republic of China (2010 editions) method, take rutin as standard substance, measure general flavone content in extract.Determination step is: 1) standard curve making: precision measures reference substance solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, puts in 25ml measuring bottle respectively, respectively adds 30% ethanol to 6.0ml, adds 5% sodium nitrite solution 1ml.Mixing, places 6 minutes, then adds 10% aluminum nitrate solution 1ml, shake up, place 6 minutes.Hydro-oxidation sodium test solution 10ml, then add 30% ethanol to scale, shake up, place 15 minutes, take corresponding reagent as blank, with ultraviolet visible spectrophotometry, measure absorbance at the wavelength place of 510nm, take absorbance as vertical coordinate, concentration is abscissa, drawing standard curve.2) get a certain amount of sample and be placed in 25ml volumetric flask, other is according to standard curve preparation process, from " adding sodium nitrite solution 1ml ", measure absorbance in accordance with the law, get need testing solution 3ml simultaneously, except not hydro-oxidation sodium test solution, all the other the same operations, as blank, calculate general flavone content (in rutin) in extract.The results are shown in Table 1.
General flavone content in table 1 Radix Ginseng seed extract
| Sample ID | General flavone content % |
| Radix Ginseng seed crude extract | 0.84 |
| Radix Ginseng seed extract A | 0.36 |
| Radix Ginseng seed extract B | 1.91 |
| Radix Ginseng seed extract C | 0.88 |
| Radix Ginseng seed extract D | 0.14 |
Embodiment 3: the detection of cell inhibitory effect activity
According to Radix Ginseng seed extract prepared by embodiment 1, being dissolved in deionized water completely, being mixed with 0.8 μ g/ml ~ 100 μ g/ml solution to be measured respectively, for measuring the inhibit activities of on cell proliferation.Determination step is: first human fibroblasts kind entered (2000 cells/well, 200 μ L) in 96 orifice plates; Cultivate and remove culture medium after 72 hours, add solution 10 μ L to be measured, cultivate after 24 hours, adopt mtt assay to pass through to measure cell relative populations thus the rate of increase calculating cell.The Cleaning Principle of mtt assay is that the succinate dehydrogenase in living cells mitochondrion can make exogenous MTT be reduced to water-insoluble bluish violet crystallization first a ceremonial jade-ladle, used in libation and be deposited in cell, and dead cell is without this function.First a ceremonial jade-ladle, used in libation in dimethyl sulfoxide energy dissolved cell, by micropore spectrophotometric determination 550nm wavelength absorption value, can reflect living cells quantity indirectly.Within the scope of certain cell number, the amount that MTT crystallization is formed is directly proportional to viable count, the results are shown in Figure 1.
As shown in Figure 1, Radix Ginseng seed extract A-D of the present invention all has the inhibitory action to skin flbroblast propagation, wherein Radix Ginseng seed extract B and Radix Ginseng seed extract C is in the concentration range of 0.8 μ g/ml ~ 100 μ g/ml, and along with the increase of concentration, inhibited proliferation strengthens.
The explanation of above embodiment just understands method of the present invention and core concept thereof for helping.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improve and modify and also fall in the protection domain of the claims in the present invention.
Claims (10)
1. a preparation method for Radix Ginseng seed extract, is characterized in that, comprising:
Step 1, Radix Ginseng seed extracting in water, obtain Radix Ginseng seed extracting solution;
Step 2, Radix Ginseng seed extracting solution are concentrated, loading is to macroporous adsorbent resin, gradient elution is carried out successively with deionized water, gradient concentration alcoholic solution from low to high, collect the eluent of deionized water wherein, 30v/v%-95v/v% concentration ethanol eluting respectively, removed under reduced pressure second alcohol and water, obtains Radix Ginseng seed extract.
2. preparation method according to claim 1, is characterized in that, comprising:
Step 1, Radix Ginseng seed extracting in water, obtain Radix Ginseng seed extracting solution;
Step 2, Radix Ginseng seed extracting solution are concentrated, loading is to macroporous adsorbent resin, gradient elution is carried out successively with deionized water, gradient concentration alcoholic solution from low to high, collect the eluent of deionized water wherein, 30v/v%, 70v/v%, 95v/v% concentration ethanol eluting respectively, removed under reduced pressure second alcohol and water, obtains Radix Ginseng seed extract.
3. preparation method according to claim 1, it is characterized in that, step 1 is:
Radix Ginseng seed is pulverized, and adds deionized water in 1g:10mL ratio, and in water-bath, 80-90 DEG C micro-ly boils, and extract twice, each 1 hour, centrifugal, supernatant liquid filtering, collects all filtrate, obtains Radix Ginseng seed extracting solution.
4. preparation method according to claim 1, it is characterized in that, described step 2 gradient concentration alcoholic solution is from low to high the gradient concentration alcoholic solution of 30v/v%-95v/v% concentration.
5. preparation method according to claim 4, it is characterized in that, described step 2 gradient concentration alcoholic solution is from low to high the gradient concentration alcoholic solution be made up of 30v/v% alcoholic solution, 70v/v% alcoholic solution and 95v/v% alcoholic solution.
6. preparation method according to claim 1, it is characterized in that, described macroporous adsorbent resin is HP-20 type macroporous adsorbent resin.
7. the Radix Ginseng seed extract prepared of preparation method described in claim 1-6 any one.
8. Radix Ginseng seed extract according to claim 7, it is characterized in that, general flavone content is 0.14-1.91%.
9. Radix Ginseng seed extract described in claim 7 is suppressing skin cell proliferation and the application in preparation skin cell proliferation inhibitor.
10. apply according to claim 9, it is characterized in that, described Skin Cell is skin flbroblast.
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Cited By (2)
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| WO2019165963A1 (en) * | 2018-02-28 | 2019-09-06 | 伽蓝（集团）股份有限公司 | Method for screening skin endogenous aging targets, active material for improving skin endogenous aging and screening method therefor |
| CN116617137A (en) * | 2023-06-27 | 2023-08-22 | 甄萃(广东)创新技术有限公司 | Ginseng seed extract and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030088417A (en) * | 2003-11-06 | 2003-11-19 | 주식회사 케이티앤지 | The memory increasing novel compound ginseno-jk from ginseng hairy roots and the manufacturing method of the ginseno-jk |
| CN1823933A (en) * | 2005-12-20 | 2006-08-30 | 北京天新园医药科技开发有限公司 | Ginseng aconite injection liquid and its preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030088417A (en) * | 2003-11-06 | 2003-11-19 | 주식회사 케이티앤지 | The memory increasing novel compound ginseno-jk from ginseng hairy roots and the manufacturing method of the ginseno-jk |
| CN1823933A (en) * | 2005-12-20 | 2006-08-30 | 北京天新园医药科技开发有限公司 | Ginseng aconite injection liquid and its preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 于明: "人参果化学成分研究", 《中国优秀硕士学位论文全文数据库》 * |
| 刘鹤松等: "人参皂苷Rg3对人成纤维细胞增殖和凋亡影响的实验研究", 《中国实验诊断学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019165963A1 (en) * | 2018-02-28 | 2019-09-06 | 伽蓝（集团）股份有限公司 | Method for screening skin endogenous aging targets, active material for improving skin endogenous aging and screening method therefor |
| CN116617137A (en) * | 2023-06-27 | 2023-08-22 | 甄萃(广东)创新技术有限公司 | Ginseng seed extract and preparation method thereof |
| CN116617137B (en) * | 2023-06-27 | 2023-10-20 | 甄萃(广东)创新技术有限公司 | Ginseng seed extract and preparation method thereof |
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