CN105231463A - Technology for extracting insoluble dietary fiber from bean dregs by enzyme process - Google Patents
Technology for extracting insoluble dietary fiber from bean dregs by enzyme process Download PDFInfo
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- CN105231463A CN105231463A CN201510744070.2A CN201510744070A CN105231463A CN 105231463 A CN105231463 A CN 105231463A CN 201510744070 A CN201510744070 A CN 201510744070A CN 105231463 A CN105231463 A CN 105231463A
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- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 108
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 58
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 58
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000005516 engineering process Methods 0.000 title abstract description 7
- 229940088598 enzyme Drugs 0.000 claims abstract description 54
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 25
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 25
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 24
- 238000000605 extraction Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000008107 starch Substances 0.000 claims description 12
- 239000008363 phosphate buffer Substances 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
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- 102000035092 Neutral proteases Human genes 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Beans For Foods Or Fodder (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention discloses a technology for extracting insoluble dietary fiber from bean dregs by an enzyme process. The processing technology comprises the following steps: preparing bean dregs, oven-drying, smashing, sieving, adding water, adding buffer solution, adding proteases, inactivating enzymes, adding alpha-amylase, inactivating enzymes, conducting centrifugal filtration, washing the filter residues, and drying the filter residues to reach a constant weight. The technology comprehensively considers pH values, substrate concentration, grinding degree, the use conditions of enzymes, the amount of enzymes used and other parameters which have effects on the extraction rate. The processing technology enables the reasonable utilization of bean dregs, and reduces environmental pollution caused by discarded beans. The experimental results of ash measurements show that the insoluble dietary fiber extracted by the enzyme process is high in purity, and has only about 2.8% ash content.
Description
Technical field
The present invention relates to technical field of food deep processing, be specifically related to the technique of insoluble dietary fiber in a kind of Enzymatic Extraction bean dregs.
Background technology
Dietary fiber refers to the polysaccharide that can not be immediately used by the body, and in the intestines and stomach of the mankind, digestive ferment cannot digest dietary fiber, therefore it is not easily absorbed by the body.This kind of polysaccharide, mainly from the complex carbohydrate of plant cell wall, also can be referred to as SNSP, i.e. the polysaccharide of non-alpha-glucans.It is divided into soluble dietary fiber and insoluble dietary fiber.The nutritive value of dietary fiber more and more receives the concern of people, also more and more deep to its research, present dietary fiber is the 7th class nutrient after this six large nutrient of protein, carbohydrate, fat, vitamin, mineral matter and water that people generally acknowledge.
China is the native place of soybean, has long soybean planting history, has a large amount of soybean for the production of various bean product, oil plant every year now.Bean dregs are the byproducts in bean product machining process, the annual wet bean dregs produced due to the processing of soybean processing industry reach 2,000 ten thousand tons, these bean dregs are all used for feeding pigs or directly discarding as feed, can turn sour again smelly to bean dregs in summer, this makes bean dregs not only become a kind of resource be wasted, and also creates environmental pollution simultaneously.
Be rich in dietary fiber and protein, vitamins and other nutritious components in bean dregs, also no problem in security, cheap, dietary fiber content is high.Have research to point out, the dietary fiber of soybean bean dregs in the application and separation and Extraction bean dregs of development functionality food has bright prospects.
The insoluble dietary fiber contained in bean dregs is higher than soluble dietary fiber, and therefore, the study on the industrialization extracting insoluble dietary fiber in bean dregs still has certain value.To in the Study on extraction of insoluble dietary fiber, the research in enzyme process is relatively less, is more the research for chemical method aspect.Few more than the technique extracting soluble dietary fiber in bean dregs to the correlative study of extracting insoluble dietary fiber in bean dregs, it is also less that relevant research is reported in document.
When adopting prior art to extract, the factor that the pH value in leaching process, concentration of substrate, degree of grinding, the service condition of enzyme and the use amount of enzyme etc. affect recovery rate cannot be considered, and, cannot insoluble dietary fiber be made comparatively to be thoroughly separated with soluble dietary fiber, the insoluble dietary fiber purity extracted be lower.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, the technique of insoluble dietary fiber in a kind of high efficiency, highly purified Enzymatic Extraction bean dregs is provided, consider the factor that the pH value in leaching process, concentration of substrate, degree of grinding, the service condition of enzyme and the use amount of enzyme etc. affect recovery rate, make the extractive technique of insoluble dietary fiber, possess industrialization prospect.
To achieve these goals, present invention employs following technical scheme:
In Enzymatic Extraction bean dregs, a technique for insoluble dietary fiber, is characterized in that, comprises the steps:
(1) bean dregs are dried pulverizing and obtain okara powder;
(2) in every gram of okara powder, add the phosphate buffer 5 ~ 7ml of distilled water 5 ~ 7ml and PH6.5 ~ 7.0, obtain bean dregs slurry;
(3) in bean dregs slurry, add neutral proteinase and carry out first time enzymolysis, the addition of neutral proteinase is step (1) described okara powder: neutral proteinase=1g:0.7 ~ 1mg, obtains the first enzymolysis bean dregs slurry;
(4) carry out first time to the first enzymolysis bean dregs slurry to go out ferment treatment, obtain the first enzyme bean dregs that go out and starch;
(5) go out in enzyme bean dregs slurry to first and add heat-resistant alpha-amylase and carry out second time enzymolysis, the addition of heat-resistant alpha-amylase is step (1) described okara powder: heat-resistant alpha-amylase=1g:10 ~ 20 μ l, obtains the second enzymolysis bean dregs and starches;
(6) carry out second time to the second enzymolysis bean dregs slurry to go out ferment treatment, obtain the second enzyme bean dregs that go out and starch;
(7) to second go out enzyme bean dregs slurry carry out centrifugation, get filter residue after filtration, by residue washing and dry after, obtain insoluble dietary fiber.
Described okara powder was 80 ~ 120 mesh sieve gained powder.
The compound method of described phosphate buffer is as follows: the potassium dihydrogen phosphate 250mL getting 0.2moL/mL, adds the 0.2moL/mL sodium hydroxide solution of 118mL, then is diluted with water to 1000mL.
The enzyme work of described neutral proteinase is 25 ~ 75U/mg
The enzyme work of described heat-resistant alpha-amylase is 200 ~ 400U/mL
Compared with prior art, the beneficial effect that possesses of the present invention:
The technique of insoluble dietary fiber in Enzymatic Extraction bean dregs disclosed by the invention, its technical process is succinct.Science, to be easy to operate and control, to comprise: bean dregs → dry → pulverize → sieve → add water → with buffer solution → the add protease → enzyme that goes out → add AMS → enzyme → centrifugal filtration of going out → washing filter residue → filter residue to dry to constant weight.
The present invention has considered the factor that pH value, concentration of substrate, degree of grinding, the service condition of enzyme and the use amount of enzyme etc. in leaching process affect recovery rate, obtain science, efficient, succinct, being easy to operate can the technology of industrialization, the invention enables bean dregs resource to obtain Appropriate application, decrease bean or pea and discard the environmental pollution caused; The goods that the present invention obtains, illustrated by the determination experiment result of ash content, the insoluble dietary fiber purity that this Enzymatic Extraction obtains is high, and content of ashes is only about 2.8%, and its purity is more than 2 times that conventional art extracts.
Accompanying drawing explanation
Fig. 1 be embodiment 1 protease addition to the influence curve figure of recovery rate, in figure, abscissa is protease addition, and ordinate is recovery rate.
Fig. 2 be embodiment 1 AMS addition to the influence curve figure of recovery rate, in figure, abscissa is AMS addition, and ordinate is recovery rate.
Detailed description of the invention
Below by embodiment, technical scheme of the present invention is further elaborated.
Embodiment 1
Reagent used by the present embodiment is as follows:
Mentioned reagent is utilized to be mixed with solution as follows:
(1) 0.05mol/LMES-TRIS buffer solution:
Take 4.3021gMES and 2.6928gTRIS, by the water-soluble solution of 374mL, then regulate pH=8.17 (during preparation, temperature 25 DEG C, adopts interpolation calculation to obtain) with the NaOH of 6mol/L, be diluted with water to 440mL.
(2) neutral protein enzyme solutions:
Be made into the neutral protein enzyme solutions of 50mg/mL with 0.05mol/LMES-TRIS buffer solution, now with the current, corresponding enzyme is lived as 2500U/mL.
(3) phosphate buffer of PH=6.8:
Get the potassium dihydrogen phosphate 250mL of 0.2moL/mL, add the 0.2moL/mL sodium hydroxide solution of 118mL, be diluted with water to 1000mL.
In the Enzymatic Extraction bean dregs of the present embodiment, the technique of insoluble dietary fiber, comprises the steps:
(1) bean dregs are dried at 75 DEG C spend the night, pulverize, cross 50 mesh sieves, obtain okara powder;
(2) okara powder (M of 3.00g is taken
1), add the phosphate buffer of 20.00mL distilled water and 20.00mLpH=6.8, obtain bean dregs slurry;
(3) add to bean dregs slurry the neutral protein enzyme solutions that 50.4 μ l MES-TRIS buffer solutions are made into 50mg/mL, in 50 DEG C of waters bath with thermostatic control, carry out first time enzymolysis 1.5h, obtain the first enzymolysis bean dregs slurry; Adding of specific neutral proteinase is that protein in bean dregs is carried out enzymolysis by this step, if enzymolysis is thorough, then will containing protein in extract, therefore, the enzymolysis of protease not only affects the recovery rate of technique, and also the purity of the insoluble dietary fiber obtained is extracted in impact.
(4) under 85 DEG C of conditions, carry out first time to the first enzymolysis bean dregs slurry to go out ferment treatment 10min, the first enzyme bean dregs that go out are starched;
(5) to first go out enzyme bean dregs slurry in add 51.0 μ l heat-resistant alpha-amylases, in 70 DEG C of waters bath with thermostatic control, carry out second time enzymolysis 1.5h, obtain second enzymolysis bean dregs starch;
(6) the second enzymolysis bean dregs slurry is boiled 10min to carry out second and to go out ferment treatment, obtain second and to go out enzyme bean dregs slurry;
(7) supercentrifuge 3000r/min is used to carry out centrifugation, by constant weight filter paper (mass M to the second enzyme bean dregs slurry that goes out
2) filter, the filter paper after filtration and filter residue are dried to constant weight (M together at 70 DEG C
3), obtain insoluble dietary fiber in described bean dregs.
1, specific neutral protease and heat-resistant alpha-amylase addition are on the impact of recovery rate
1.1, test method
The okara powder taking 8 parts of 3.00g is tested, and 8 groups of tests is numbered respectively 1. 2. 3. 4. 5. 6. 7. 8., and each group test is except step (3) is different with the condition of step (5), and all the other steps are all consistent.
For step (3), be numbered 1. 2. 3. test 4. add neutral proteinase respectively by the addition of 42U/g, 84U/g, 126U/g and 168U/g successively; Be numbered 5. 6. 7. test 8. all add neutral proteinase by the addition of 42U/g.
For step (5), be numbered 1. 2. 3. test 4. all add heat-resistant alpha-amylase by the addition of 42U/g; Be numbered 5. 6. 7. test 8. add heat-resistant alpha-amylase respectively by the addition of 5U/g, 10U/g, 15U/g and 20U/g successively.
Finally, according to formula:
calculate recovery rate, in formula: M
1for sample quality, unit is gram (g); M
2for filter paper quality, unit is gram (g); M
3for the gross mass of filter paper after constant weight and filter residue, unit is gram (g).
1.2 interpretation of result
1.2.1, protease addition affects interpretation of result to recovery rate
As shown in Figure 1, recovery rate is along with the increase of the addition of protease, first slowly improve, after enzyme amount addition arrives 84U/g, recovery rate improves rapidly along with the increase of protease addition, when protease addition is 126U/g, recovery rate is maximum, and subsequently along with the increase of protease addition, recovery rate reduces.
1.2.2, AMS addition affects interpretation of result to recovery rate
As shown in Figure 2, along with the increase of AMS addition, extract and take the lead in improving, when AMS addition is 10U/g, recovery rate is maximum, reaches 86.6%, and then along with the increase of AMS addition, recovery rate starts to reduce.
2, the orthogonal experiment of optimal extract process is determined
Orthogonal experiment selects the granularity (A) of okara powder, protease addition (B) and AMS addition (C) three factors, using recovery rate as index, carry out the orthogonal experiment of Three factors-levels, orthogonal experiment factor level table is as shown in table 2.
Each tested number takes 3.0000g okara powder sample, each group of test except the granularity (A) of okara powder, protease addition (B) all identical with embodiment 1 with all the other technological parameters of AMS addition (C), experimental result is as shown in table 3.
Table 2 orthogonal experiment factor level table
Table 3 Orthogonal experiment results and analysis
Draw from table 3, optimal extract process is first group of combination A
1b
1c
1, namely degree of grinding selected 50 mesh sieves, and protease addition is 42U/g, and AMS addition is 5U/g.Extraction rate reached corresponding to this extracting factor is to 83.6%.Know from the analysis of extreme difference R value, the primary and secondary order that affects of three factors on extraction rate is: alphalise starch enzyme amount > degree of grinding > albumen enzyme amount again.
3, the content of ashes of the insoluble dietary fiber obtained is extracted
3.1, the assay method of content of ashes
Take the okara powder sample that 3 parts of 3.00g cross 50 mesh sieves, test by processing step described in the present embodiment, in experiment, protease enzyme addition is 84U/g, and AMS enzyme addition is 10U/g.The 3 parts of insoluble dietary fiber CEs obtained respectively are taken 1.0000g as sample, carries out the mensuration of ash content according to the mensuration of ash content " in the GB50094-2010 national food safety standard food ".
3.2, interpretation of result
The measurement result of content of ashes is as shown in table 4.
The mensuration of content of ashes in table 4 insoluble dietary fiber
Draw from table 4, content of ashes in the insoluble dietary fiber obtained is extracted lower by present invention process, impurity content about about 2.8% generally, illustrates that in insoluble dietary fiber, impurity content is less, and the purity of insoluble dietary fiber is more than 2 times that conventional art extracts.
Embodiment 2
In Enzymatic Extraction bean dregs, a technique for insoluble dietary fiber, comprises the steps:
(1) bean dregs are dried pulverizing and obtain okara powder;
(2) in every gram of okara powder, add the phosphate buffer 5ml of distilled water 5ml and PH6.5, obtain bean dregs slurry;
(3) in bean dregs slurry, add neutral proteinase and carry out first time enzymolysis, the addition of neutral proteinase is step (1) described okara powder: neutral proteinase=1g:0.7mg, obtains the first enzymolysis bean dregs slurry;
(4) carry out first time to the first enzymolysis bean dregs slurry to go out ferment treatment, obtain the first enzyme bean dregs that go out and starch;
(5) go out in enzyme bean dregs slurry to first and add heat-resistant alpha-amylase and carry out second time enzymolysis, the addition of heat-resistant alpha-amylase is step (1) described okara powder: heat-resistant alpha-amylase=1g:10 μ l, obtains the second enzymolysis bean dregs and starches;
(6) carry out second time to the second enzymolysis bean dregs slurry to go out ferment treatment, obtain the second enzyme bean dregs that go out and starch;
(7) to second go out enzyme bean dregs slurry carry out centrifugation, get filter residue after filtration, by residue washing and dry after, obtain insoluble dietary fiber in described bean dregs.
Step (1) described okara powder was 80 mesh sieve gained powder.
The enzyme of neutral proteinase is lived as 25U/mg
The enzyme of heat-resistant alpha-amylase is lived as 200U/mL.
Embodiment 3
In Enzymatic Extraction bean dregs, a technique for insoluble dietary fiber, comprises the steps:
(1) bean dregs are dried pulverizing and obtain okara powder;
(2) in every gram of okara powder, add the phosphate buffer 7ml of distilled water 7ml and PH7.0, obtain bean dregs slurry;
(3) in bean dregs slurry, add neutral proteinase and carry out first time enzymolysis, the addition of neutral proteinase is step (1) described okara powder: neutral proteinase=1g:1mg, obtains the first enzymolysis bean dregs slurry;
(4) carry out first time to the first enzymolysis bean dregs slurry to go out ferment treatment, obtain the first enzyme bean dregs that go out and starch;
(5) go out in enzyme bean dregs slurry to first and add heat-resistant alpha-amylase and carry out second time enzymolysis, the addition of heat-resistant alpha-amylase is step (1) described okara powder: heat-resistant alpha-amylase=1g:20 μ l, obtains the second enzymolysis bean dregs and starches;
(6) carry out second time to the second enzymolysis bean dregs slurry to go out ferment treatment, obtain the second enzyme bean dregs that go out and starch;
(7) to second go out enzyme bean dregs slurry carry out centrifugation, get filter residue after filtration, by residue washing and dry after, obtain insoluble dietary fiber in described bean dregs.
Step (1) described okara powder was 120 mesh sieve gained powder.
The enzyme of neutral proteinase is lived as 75U/mg
The enzyme of heat-resistant alpha-amylase is lived as 400U/mL.
Embodiment 4
In Enzymatic Extraction bean dregs, a technique for insoluble dietary fiber, comprises the steps:
(1) bean dregs are dried pulverizing and obtain okara powder;
(2) in every gram of okara powder, add the phosphate buffer 6ml of distilled water 6ml and PH6.7, obtain bean dregs slurry;
(3) in bean dregs slurry, add neutral proteinase and carry out first time enzymolysis, the addition of neutral proteinase is step (1) described okara powder: neutral proteinase=1g:0.9mg, obtains the first enzymolysis bean dregs slurry;
(4) carry out first time to the first enzymolysis bean dregs slurry to go out ferment treatment, obtain the first enzyme bean dregs that go out and starch;
(5) go out in enzyme bean dregs slurry to first and add heat-resistant alpha-amylase and carry out second time enzymolysis, the addition of heat-resistant alpha-amylase is step (1) described okara powder: heat-resistant alpha-amylase=1g:15 μ l, obtains the second enzymolysis bean dregs and starches;
(6) carry out second time to the second enzymolysis bean dregs slurry to go out ferment treatment, obtain the second enzyme bean dregs that go out and starch;
(7) to second go out enzyme bean dregs slurry carry out centrifugation, get filter residue after filtration, by residue washing and dry after, obtain insoluble dietary fiber in described bean dregs.
Step (1) described okara powder was 100 mesh sieve gained powder.
The enzyme of neutral proteinase is lived as 45U/mg
The enzyme of heat-resistant alpha-amylase is lived as 280U/mL.
More than show and describe general principle of the present invention and principal character and advantage of the present invention, other adopt similar step and the embodiment of mode, state no longer one by one.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and description just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (5)
1. the technique of insoluble dietary fiber in Enzymatic Extraction bean dregs, it is characterized in that, it comprises the steps:
(1) bean dregs are dried pulverizing and obtain okara powder;
(2) in every gram of okara powder, add the phosphate buffer 5 ~ 7ml of distilled water 5 ~ 7ml and PH6.5 ~ 7.0, obtain bean dregs slurry;
(3) in bean dregs slurry, add neutral proteinase and carry out first time enzymolysis, the addition of neutral proteinase is step (1) described okara powder: neutral proteinase=1g:0.7 ~ 1mg, obtains the first enzymolysis bean dregs slurry;
(4) carry out first time to the first enzymolysis bean dregs slurry to go out ferment treatment, obtain the first enzyme bean dregs that go out and starch;
(5) go out in enzyme bean dregs slurry to first and add heat-resistant alpha-amylase and carry out second time enzymolysis, the addition of heat-resistant alpha-amylase is step (1) described okara powder: heat-resistant alpha-amylase=1g:10 ~ 20 μ l, obtains the second enzymolysis bean dregs and starches;
(6) carry out second time to the second enzymolysis bean dregs slurry to go out ferment treatment, obtain the second enzyme bean dregs that go out and starch;
(7) to second go out enzyme bean dregs slurry carry out centrifugation, get filter residue after filtration, by residue washing and dry after, obtain the insoluble dietary fiber in described bean dregs.
2. the technique of insoluble dietary fiber in Enzymatic Extraction bean dregs as claimed in claim 1, it is characterized in that, step (1) described okara powder was 80 ~ 120 mesh sieve gained powder.
3. the technique of insoluble dietary fiber in Enzymatic Extraction bean dregs as claimed in claim 1, it is characterized in that, the compound method of step (2) described phosphate buffer is as follows: the potassium dihydrogen phosphate 250mL getting 0.2moL/mL, add the 0.2moL/mL sodium hydroxide solution of 118mL, then be diluted with water to 1000mL.
4. the technique of insoluble dietary fiber in Enzymatic Extraction bean dregs as claimed in claim 1, it is characterized in that, the enzyme work of described neutral proteinase is 25 ~ 75U/mg.
5. the technique of insoluble dietary fiber in Enzymatic Extraction bean dregs as claimed in claim 1, it is characterized in that, the enzyme work of described heat-resistant alpha-amylase is 200 ~ 400U/mL.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105361189A (en) * | 2015-11-10 | 2016-03-02 | 广西大学 | Extraction technology for water-insoluble plant dietary fiber |
| CN106092815A (en) * | 2016-06-27 | 2016-11-09 | 江西出入境检验检疫局检验检疫综合技术中心 | The assay method of total dietary fiber in a kind of food |
| CN109170919A (en) * | 2018-10-16 | 2019-01-11 | 克东禹王大豆蛋白食品有限公司 | A kind of method of surfactant auxiliary Enzymatic Extraction bean dregs |
| CN110101087A (en) * | 2019-04-09 | 2019-08-09 | 夏建伟 | A kind of preparation method of the food fibre powder containing peptide and the food fibre powder containing peptide of preparation |
| CN111449202A (en) * | 2020-04-03 | 2020-07-28 | 吉林农业大学 | Soybean insoluble dietary fiber and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103976370A (en) * | 2014-05-28 | 2014-08-13 | 彭常安 | Production method of purple sweet potato residue dietary fibers |
| CN103976369A (en) * | 2014-04-04 | 2014-08-13 | 浙江恒乐粮食有限公司 | Production method for high-activity rice bran dietary fiber |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103976369A (en) * | 2014-04-04 | 2014-08-13 | 浙江恒乐粮食有限公司 | Production method for high-activity rice bran dietary fiber |
| CN103976370A (en) * | 2014-05-28 | 2014-08-13 | 彭常安 | Production method of purple sweet potato residue dietary fibers |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105361189A (en) * | 2015-11-10 | 2016-03-02 | 广西大学 | Extraction technology for water-insoluble plant dietary fiber |
| CN106092815A (en) * | 2016-06-27 | 2016-11-09 | 江西出入境检验检疫局检验检疫综合技术中心 | The assay method of total dietary fiber in a kind of food |
| CN109170919A (en) * | 2018-10-16 | 2019-01-11 | 克东禹王大豆蛋白食品有限公司 | A kind of method of surfactant auxiliary Enzymatic Extraction bean dregs |
| CN109170919B (en) * | 2018-10-16 | 2022-04-01 | 克东禹王大豆蛋白食品有限公司 | Method for extracting bean dregs by surfactant-assisted enzyme method |
| CN110101087A (en) * | 2019-04-09 | 2019-08-09 | 夏建伟 | A kind of preparation method of the food fibre powder containing peptide and the food fibre powder containing peptide of preparation |
| CN111449202A (en) * | 2020-04-03 | 2020-07-28 | 吉林农业大学 | Soybean insoluble dietary fiber and application thereof |
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