CN105231249A - Bamboo leaf flavone extracting method by synergy of ultrasonic and surface-active agent - Google Patents
Bamboo leaf flavone extracting method by synergy of ultrasonic and surface-active agent Download PDFInfo
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- CN105231249A CN105231249A CN201510595028.9A CN201510595028A CN105231249A CN 105231249 A CN105231249 A CN 105231249A CN 201510595028 A CN201510595028 A CN 201510595028A CN 105231249 A CN105231249 A CN 105231249A
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- 229930003944 flavone Natural products 0.000 title claims abstract description 44
- 235000011949 flavones Nutrition 0.000 title claims abstract description 44
- 239000004094 surface-active agent Substances 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 29
- 235000017166 Bambusa arundinacea Nutrition 0.000 title claims abstract description 23
- 235000017491 Bambusa tulda Nutrition 0.000 title claims abstract description 23
- 241001330002 Bambuseae Species 0.000 title claims abstract description 23
- 235000015334 Phyllostachys viridis Nutrition 0.000 title claims abstract description 23
- 239000011425 bamboo Substances 0.000 title claims abstract description 23
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title abstract description 5
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title abstract description 5
- 150000002212 flavone derivatives Chemical class 0.000 title abstract 4
- 238000000605 extraction Methods 0.000 claims abstract description 30
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000007787 solid Substances 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 17
- 239000011259 mixed solution Substances 0.000 claims abstract description 3
- 150000002213 flavones Chemical class 0.000 claims description 39
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 claims description 11
- 239000000693 micelle Substances 0.000 claims description 9
- 239000007864 aqueous solution Substances 0.000 claims description 8
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 6
- -1 neopelex Chemical compound 0.000 claims description 6
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 claims description 3
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 3
- 125000000217 alkyl group Chemical group 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims description 3
- GGHPAKFFUZUEKL-UHFFFAOYSA-M sodium;hexadecyl sulfate Chemical compound [Na+].CCCCCCCCCCCCCCCCOS([O-])(=O)=O GGHPAKFFUZUEKL-UHFFFAOYSA-M 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 5
- 239000002904 solvent Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 238000000638 solvent extraction Methods 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 238000002604 ultrasonography Methods 0.000 abstract 1
- 238000002137 ultrasound extraction Methods 0.000 description 11
- 239000000243 solution Substances 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 8
- 238000011084 recovery Methods 0.000 description 8
- 229930014626 natural product Natural products 0.000 description 4
- 102000011759 adducin Human genes 0.000 description 3
- 108010076723 adducin Proteins 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 238000000967 suction filtration Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 229930013930 alkaloid Chemical class 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000002390 cell membrane structure Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 150000002926 oxygen Chemical class 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Chemical class 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Cosmetics (AREA)
Abstract
The present invention discloses a bamboo leaf flavone extracting method by synergy of ultrasonic and surface-active agent. The method includes the following steps: bamboo leaf powder is added to a mixed solution of ethanol and extraction liquid, the liquid to solid ratio of the extraction liquid to the bamboo leaf powder is 15-20 : 1 mL/g, the liquid to solid ratio of the ethanol and the bamboo leaf powder is 15-20 : 1 mL/g; and the mixture is subjected to a synergic extraction of ultrasonic and surface-active agent at an ultrasound frequency of 40 KHz and an ultrasonic power of 100-450 W for 1-3 times with 20-30 minutes each time to obtain the bamboo leaf flavone. The extraction method has the advantages of being low in equipment requirement, convenient in operation, short in extraction time, needing no heating, and being conductive to protect heat labile component, which solves the problems that the existing solvent extraction method of bamboo leaf flavone is large in solvent consumption volume, is low in efficiency and is high in cost.
Description
Technical field
The invention belongs to food additives field, be specifically related to a kind of ultrasonic wave-surfactant and work in coordination with the method extracting bamboo-leaves flavones.
Background technology
From last century the eighties, researcher is to having found many natural products in the research of bamboo extractive composition, comprise flavones/flavonoids, polysaccharide, phenolic acid compound, alkaloid, peptide class, Anthraquinones, terpene lactone etc., wherein flavone compound (is called as bamboo-leaves flavones, BambooLeavesFlavonids) not only in bamboo extractive, content is higher, and because of its stronger biological action, comprise scavenging activated oxygen, restraining and sterilizing bacteria, anti-inflammatory, detumescence, reducing blood lipid etc., cause the extensive concern of Chinese scholars.AOB (AntioxidantofBambooLeaves) went through in 2004 to list in " People's Republic of China's food additives use sanitary standard " (GB2760), and existing permission uses as antioxidant from natural food in the food such as edible oil, meat products, flavouring, fruit juice, cake.
The traditional extraction process of bamboo-leaves flavones uses solvent method.Use extract by solvents bamboo-leaves flavones, solvent-oil ratio is large, efficiency is low, cost is high.
Summary of the invention
For overcoming the deficiency of solvent extraction method, the invention provides a kind of ultrasonic wave-surfactant and working in coordination with the method extracting bamboo-leaves flavones.
The object of the invention is achieved through the following technical solutions:
The method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant, comprise the following steps: leaf of bamboo powder is joined in the mixed solution of ethanol and extract, the liquid-solid ratio of extract and leaf of bamboo powder is 15 ~ 20:1mL/g, and the liquid-solid ratio of ethanol and leaf of bamboo powder is 15 ~ 20:1mL/g; Be under the condition of 40kHz in supersonic frequency, carry out ultrasonic wave-surfactant with ultrasonic power 100 ~ 450W and work in coordination with extraction 1 ~ 3 time, obtain bamboo-leaves flavones.
Described ultrasonic wave-surfactant is worked in coordination with and extracted each time of extracting is 20 ~ 30min.
The number of times that extraction worked in coordination with by described ultrasonic wave-surfactant is preferably 2 times.
The temperature that extraction worked in coordination with by described ultrasonic wave-surfactant is normal temperature.
Normal temperature of the present invention is 26 ~ 30 DEG C.
Described ethanol to be mass percent be 95% ethanol.
The liquid-solid ratio of described extract and leaf of bamboo powder is preferably 20:1mL/g, and the liquid-solid ratio of ethanol and leaf of bamboo powder is 20:1mL/g.
Described extract is the aqueous solution of the Tween-20 of critical micelle concentration (cmc), Tween-80, OP-10, softex kw, lauryl sodium sulfate, neopelex, bromide sixteen alkyls pyridine or sodium hexadecyl sulfate.
Described extract preferred concentration is the lauryl sodium sulfate aqueous solution of 0.5 ~ 3.0 times of critical micelle concentration (cmc).
Accurate title 2.0g gets leaf of bamboo powder, respectively with the Tween-20 of critical micelle concentration (cmc), the aqueous solution of Tween-80, OP-10, softex kw, lauryl sodium sulfate, neopelex, bromide sixteen alkyls pyridine, sodium hexadecyl sulfate is extract, under supersonic frequency is 40kHz and normal temperature, with ultrasonic extraction power 300W, extraction time 30min, liquid-solid ratio 15:1 (mL/g), extract 1 bamboo-leaves flavones.After extraction terminates, calculate bamboo-leaves flavones recovery rate, compare the extraction effect of different surfaces activating agent, the surfactant filtering out extraction effect the best is lauryl sodium sulfate.
Under ultrasonic extraction frequency 40kHz and normal temperature, according to following extraction conditions: extraction time 30min, ultrasonic extraction power 300W, liquid-solid ratio 15:1 (mL/g), the sodium dodecyl sulfate solution of application variable concentrations, inquires into sodium dodecyl sulfate solution concentration to the impact of bamboo-leaves flavones recovery rate.When sodium dodecyl sulfate solution concentration is 0.5 ~ 3.0cmc, along with the increase of sodium dodecyl sulfate solution concentration, bamboo-leaves flavones recovery rate also improves thereupon.This is because the Micelle formed in system is many, the solubilization of bamboo-leaves flavones is strengthened.But after sodium dodecyl sulfate solution concentration is higher than 3.0cmc, bamboo-leaves flavones recovery rate declines gradually.Its reason may be that the micella that the lauryl sodium sulfate in extract is formed is too tight, limits the stripping of bamboo-leaves flavones, causes recovery rate to decline.Therefore, sodium dodecyl sulfate solution concentration is unsuitable excessive, selects 2.0 ~ 3.0cmc to be the sodium dodecyl sulfate solution concentration range be comparatively suitable for.
Described ultrasonic power is preferably 350 ~ 450W, is more preferably 400W.
Under ultrasonic extraction frequency 40kHz and normal temperature, according to following extraction conditions: extraction time 30min, sodium dodecyl sulfate solution concentration 1cmc, liquid-solid ratio 15:1 (mL/g), under different ultrasonic extraction power, inquire into ultrasonic extraction power to the impact of bamboo-leaves flavones recovery rate.When ultrasonic extraction power is between 100 ~ 400W, bamboo-leaves flavones recovery rate is directly proportional to ultrasonic extraction power.But after ultrasonic extraction power is more than 400W, bamboo-leaves flavones recovery rate slightly declined.Its reason may be in certain power bracket, along with the increase of ultrasonic power, " cavitation " accelerates the fragmentation of cell and the stripping of bamboo-leaves flavones on the one hand, but excessive ultrasonic power also can be larger to the destruction of the micellar structure that lauryl sodium sulfate is formed in extract, is unfavorable for the stripping of bamboo-leaves flavones.Therefore under considering, ultrasonic extraction power is unsuitable too high, is preferably 350 ~ 450W and can reaches better extraction effect, most preferably be 400W.
Compared with prior art, the present invention has the following advantages and beneficial effect:
Ultrasonic extraction is that the shock wave that produces of " cavitation " utilizing ultrasonic wave to cause in medium and jet are to destroy cell membrane and membrane structure, and then destroy whole plant cell, thus promote that cellular plasm is by cell membrane and cell membrane, be dissolved in the middle of medium, contribute to the extraction of active material.Extracting method of the present invention has that equipment requirement is low, easy to operate, extraction time is short, without the need to heating, the advantage being conducive to protecting thermally labile component, solves the shortcoming that existing extract by solvents bamboo-leaves flavones solvent-oil ratio is large, efficiency is low, cost is high.
For the leaching process of natural products in plant, be first extract soaking plant tissue.The surface tension close relation with extract and plant tissue can be soaked; Surface tension is larger, and extract is infiltration of plants tissue not easily, and extracting wherein natural products will be very slow or impossible, and efficiency is lower; Add surfactant in extracting method of the present invention and then effectively can reduce surface tension, make plant tissue easily be extracted liquid and fully infiltrate, thus acceleration extract gos deep into inside plant tissues, surrounds plant tissue cell; Then, natural products is dissolved in extract according to diffusion principle, and then reaches the object of extraction.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
The method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant, comprise the following steps: by 2.0g leaf of bamboo powder, put into tool plug ground conical flask, add 95% (quality) ethanol by liquid-solid ratio 20:1 (mL/g), add extract (extract is the lauryl sodium sulfate aqueous solution of 2.5 times of critical micelle concentrations (cmc)) by liquid-solid ratio 20:1 (mL/g); Then be under the condition of 40kHz and normal temperature in supersonic frequency, carry out ultrasonic wave-surfactant with ultrasonic power 400W and work in coordination with extraction 2 times, each extraction time is 20min, suction filtration, merging filtrate, is settled to 100mL, and adopting calibration curve method to calculate bamboo-leaves flavones yield is 1.88%.
Embodiment 2
The method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant, comprise the following steps: by 2.0g leaf of bamboo powder, put into tool plug ground conical flask, add 95% (quality) ethanol by liquid-solid ratio 20:1 (mL/g), add extract (extract is the lauryl sodium sulfate aqueous solution of 2.5 times of critical micelle concentrations (cmc)) by liquid-solid ratio 20:1 (mL/g); Then be under the condition of 40kHz and normal temperature in supersonic frequency, carry out ultrasonic wave-surfactant with ultrasonic power 350W and work in coordination with extraction 2 times, each extraction time is 20min, suction filtration, merging filtrate, is settled to 100mL, and adopting calibration curve method to calculate bamboo-leaves flavones yield is 1.70%.
Embodiment 3
The method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant, comprise the following steps: by 2.0g leaf of bamboo powder, put into tool plug ground conical flask, add 95% (quality) ethanol by liquid-solid ratio 20:1 (mL/g), add extract (extract is the lauryl sodium sulfate aqueous solution of 2.5 times of critical micelle concentrations (cmc)) by liquid-solid ratio 20:1 (mL/g); Then be under the condition of 40kHz and normal temperature in supersonic frequency, carry out ultrasonic wave-surfactant with ultrasonic power 450W and work in coordination with extraction 2 times, each extraction time is 20min, suction filtration, merging filtrate, is settled to 100mL, and adopting calibration curve method to calculate bamboo-leaves flavones yield is 1.78%.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. the method extracting bamboo-leaves flavones worked in coordination with by ultrasonic wave-surfactant, it is characterized in that, comprise the following steps: leaf of bamboo powder is joined in the mixed solution of ethanol and extract, the liquid-solid ratio of extract and leaf of bamboo powder is 15 ~ 20:1mL/g, and the liquid-solid ratio of ethanol and leaf of bamboo powder is 15 ~ 20:1mL/g; Be under the condition of 40kHz in supersonic frequency, carry out ultrasonic wave-surfactant with ultrasonic power 100 ~ 450W and work in coordination with extraction 1 ~ 3 time, obtain bamboo-leaves flavones.
2. the method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant according to claim 1, it is characterized in that, described ultrasonic wave-surfactant is worked in coordination with and extracted each time of extracting is 20 ~ 30min.
3. the method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant according to claim 1, and it is characterized in that, the number of times that extraction worked in coordination with by described ultrasonic wave-surfactant is 2 times.
4. the method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant according to claim 1, and it is characterized in that, the temperature that extraction worked in coordination with by described ultrasonic wave-surfactant is normal temperature.
5. the method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant according to claim 1, and it is characterized in that, the liquid-solid ratio of described extract and leaf of bamboo powder is 20:1mL/g, and the liquid-solid ratio of ethanol and leaf of bamboo powder is 20:1mL/g.
6. the method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant according to claim 1, it is characterized in that, described extract is the aqueous solution of the Tween-20 of critical micelle concentration, Tween-80, OP-10, softex kw, lauryl sodium sulfate, neopelex, bromide sixteen alkyls pyridine or sodium hexadecyl sulfate.
7. the method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant according to claim 6, and it is characterized in that, described extract is the lauryl sodium sulfate aqueous solution of 0.5 ~ 3.0 times of critical micelle concentration.
8. the method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant according to claim 1, and it is characterized in that, described ultrasonic power is 350 ~ 450W.
9. the method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant according to claim 8, and it is characterized in that, described ultrasonic power is 400W.
10. the method extracting bamboo-leaves flavones worked in coordination with by a kind of ultrasonic wave-surfactant according to claim 1, and it is characterized in that, the mass percent of described ethanol is 95%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510595028.9A CN105231249A (en) | 2015-09-17 | 2015-09-17 | Bamboo leaf flavone extracting method by synergy of ultrasonic and surface-active agent |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510595028.9A CN105231249A (en) | 2015-09-17 | 2015-09-17 | Bamboo leaf flavone extracting method by synergy of ultrasonic and surface-active agent |
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| CN105231249A true CN105231249A (en) | 2016-01-13 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107595909A (en) * | 2017-11-10 | 2018-01-19 | 鲁东大学 | A kind of method that flavone compound is extracted from Folium Panacis Quinquefolii |
| CN112618475A (en) * | 2021-01-22 | 2021-04-09 | 上海加新生物科技有限公司 | Anti-inflammatory, anti-allergic and itching-relieving plant composition and preparation method and application thereof |
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| CN107595909A (en) * | 2017-11-10 | 2018-01-19 | 鲁东大学 | A kind of method that flavone compound is extracted from Folium Panacis Quinquefolii |
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Application publication date: 20160113 |