CN105230520A - Full-automatic rat maze behavior training system - Google Patents
Full-automatic rat maze behavior training system Download PDFInfo
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- CN105230520A CN105230520A CN201510440891.7A CN201510440891A CN105230520A CN 105230520 A CN105230520 A CN 105230520A CN 201510440891 A CN201510440891 A CN 201510440891A CN 105230520 A CN105230520 A CN 105230520A
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- labyrinth
- maze
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
- A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
- A01K15/027—Exercising equipment, e.g. tread mills, carousels
Abstract
The invention discloses a full-automatic rat maze behavior training system mainly composed of a maze body, a maze inner chamber, a maze control circuit, a power source, a PC1 interface data acquisition card, and a computer. The system is characterized in that the maze body is a double-square-shaped maze composed of four maze single chambers. The maze inner chamber is positioned at the center of the double-square-shaped maze. The maze control circuit and the power source are arranged in the maze inner chamber. The maze control circuit is connected with the computer through the PC1 interface data acquisition card. A host computer control software module is arranged in the computer. The system has the advantages of low research and development cost, high practicality, simple operation, stable and reliable working, high expandability, and simple system structure. With the system, rat/mouse behavior training experiment complexity can be greatly reduced, experiment result reliability can be improved, and rat/mouse behavior training experiment automation can be realized. The system can be well applied into behavioral experiments of other animals.
Description
Technical field
The invention belongs to Animal Behavior Science and neural electrophysiology experiment field, be specifically related to a kind of full-automatic mouse labyrinth Behavioral training system.
Background technology
Labyrinth (as plus maze, Y/T labyrinth, back-shaped labyrinth, eight arm labyrinths) training is the basic means that tooth class animal learning and memory capability are gnawed in research.Also conventional in some other field, as carried out cognitive function qualification to through genetic manipulation animal, cognitive drug screening etc.
In the past when carrying out this kind of labyrinth to animal, the method adopting expert along training more.It is a very common training program as trained based on the intelligent of ageing mice of maze and memory.But when training under artificial condition, experimenter needs to do a lot of work, such as ceaselessly to record experimental result, also such as experimenter wants a hand-held baffle plate to be first limited in by rat in initiation region and to remove baffle plate etc. after the lag phase of regulation sometimes, if will change the award amount of experiment and risk probability also will change work to whole experimental situation again in a large number.Therefore doing a training experiment often needs multi-person synergy to complete.This method requires that experimenter has higher experiment skill excessively stress to avoid as far as possible causing animal.Obviously, the objectivity affecting result largely of this experimentation meeting.
In addition, along with the continuous progress of the methods such as behavior electrophysiology, simple animal behavioral study can not meet the demand of researcher.When carrying out electro physiology observation and record to the animal be in task operating, carrying out artificial contact to animal will cause great interference to observation.The automation of Behavioral training and programme-controlled be inevitable requirement when electrophysiological recording carries out to free moving animals.
Summary of the invention
The object of the invention is to the labyrinth channel door on basis, traditional labyrinth install program control mark (LED), program control award control valve, multipair red sending and receiving sensor and PC control software additional, adopt the mode of software and hardware combining, realize the automation of Behavioral training, solve the problem mentioned in above-mentioned background technology, the shape realizing large/mouse is the automation of training experiment.
The technical solution used in the present invention is; A kind of full-automatic mouse labyrinth Behavioral training system, primarily of room, labyrinth control circuit, power supply, PC1 interface data capture card and computer composition in labyrinth main body, labyrinth; It is characterized in that; Labyrinth main body is the hollow labyrinth of four labyrinth single chamber compositions, be room in labyrinth in the middle of hollow labyrinth, in labyrinth, indoor are equipped with labyrinth control circuit and power supply, and labyrinth control circuit is connected with computer by PC1 interface data capture card, and computer is built-in with PC control software module.
Labyrinth of the present invention single chamber has two room, road doors, is built-in with two road passage indicating doors, in the middle of two road passage indicating doors, arrange one piece of interchannel dividing plate, is provided with one nearby rewards point of release at interchannel dividing plate.
There are three infrarede emitting diodes and three infrared receiving tubes in single chamber indoor, labyrinth of the present invention, directly over the passage indicating door being installed on two feeder connection places respectively and reward the position of two sides about point of release.
Passage indicating door of the present invention is provided with multiple LED light emitting diode, PC control software module in computer can control the light on and off of each LED light emitting diode, greatly/mouse selects the award regular corresponding award amount of the door of different bright light number by being set in advance, and records training result.Passage indicating door is removable and replaceable, can for different training programs install can not door, access door is installed on feeder connection place by draw-in groove.
Labyrinth of the present invention single chamber arranges some infrared probes, for detecting the position of mouse in labyrinth and the passage of the actual walking of mouse.
Near award point of release of the present invention, award control valve being set, being controlled for giving the award of mouse corresponding to its behavior by the PC control software module in computer.
In labyrinth of the present invention, room is rewarded and a high definition network head with erection for installing labyrinth control circuit.
The interface of PC control software module of the present invention mainly contains training mode setting area, rewards regular setting area, other optimum configurations district of system, button area and training result recording areas; Training mode setting area is for arranging the concrete training mode of system, and different patterns has different award rules to different behaviors; Reward regular setting area for setting when the minimum award amount under associative mode and risk probability etc.; Other optimum configurations district of system is used for other parameter of initialization system, the experiment number/time such as will trained; It is EXCEL file that button area is used for starting/suspending training experiment and preserve experimental result; Training result recording areas is used for storing temporarily and shows the outcome record of current experiment.
Control circuit of the present invention mainly wraps the circuit of suitable infrared acquisition, the control circuit of LED light emitting diode is given into award control circuit; Wherein, single VCC (DC5V) end transmitting terminal D3 positive pole of the infrared probe of the circuit of infrared acquisition being connect to power supply, the ground terminal GND of power supply is met after negative pole series connection protective resistance R18, the receiving terminal DS3 negative pole of infrared probe connects the VCC end of power supply, positive pole separates two lines, article one, connect the ground terminal of power supply after line serial resistance R17, a line connects as analog signal input exports to data collecting card; In the control circuit of the LED light emitting diode on single access door, R1, R5, R6, R19 are the resistance in 330 Europe, and L1 is white light emitting diode, and Q1 is triode; R6 mono-termination power VCC, one termination L1 positive pole, R5 and R6 is in parallel, L1 negative pole connects the colelctor electrode (c pole) of three collector Q1, the emitter stage of Q1 meets power supply GND and holds, and R1 and L1 is in parallel, and base stage is connected with R19 one end, the R19 other end is connected with the digital I/O delivery outlet of capture card, and capture card can control the light on and off of corresponding LED by the level drawing high/set low corresponding I/O mouth; In single award control circuit figure, J1 is a magnetic valve, and U1 is relay, and D4 is an earial drainage diode; The negative pole (black) of magnetic valve connects the-12V end of power supply, positive pole (red) is connected with power supply+12V by relay, control coil one end of relay is connected with one of capture card digital I/O delivery outlet, and be connected with the negative pole of earial drainage diode D4, D4 just and crossing with the relay control coil other end after ground connection.
Concrete operation method of the present invention is as follows: for mouse Behavioral training, and specific tasks flow process has;
(1), before task starts, programme-control is by all LED of each room and reward control valve closedown;
(2) trainer sets training mode and trains relevant parameter (as risk probability, experiment number, award amount etc.) in PC control software module;
(3) training is clicked starting in computer and is trained button and an experimental subjects (mouse) is put in a laboratory wherein;
(4) ensuing training is all that system completes automatically, until the experiment number reaching trainer's setting stops, ejection training is completed prompting message window by PC control software module;
(5) whether trainer is according to the actual conditions judgment experiment result of whole experimentation effectively and whether preserve this experimental result;
(6) from experiment labyrinth, take out mouse, close computer, namely close all LED and reward control valve, completing a training experiment.Be more than groundwork process of the present invention, the work that trainer will do is quite simple, achieves the automation of mouse Behavioral training.
Core of the present invention is that mark (light on and off of the LED) number on access door changes automatically, effective realization of the automatic adjustment of award amount and the automatic recording function to experimental result; Install LED additional to indicate the difference award amount entering this passage in each passway in original labyrinth and reward risk; Whole training process is substantially without the need to artificial participation, and training mode and award rule change by software flexible.
The invention has the beneficial effects as follows; R&D costs are low, practical, simple to operate, working stability is reliable, autgmentability is strong, system architecture is simplely bought, greatly can simplify the complexity of large/mouse Behavioral training experiment, increase the reliability of its experimental result, the shape realizing large/mouse is the automation of training experiment; This system can also be applied in the Behaviors survey of other animal well.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is the overall structure schematic diagram of labyrinth of the present invention Behavioral training system.
Fig. 2 is the back-shaped labyrinth structure schematic diagram of labyrinth of the present invention Behavioral training system.
Fig. 3 is the labyrinth single cell structure schematic diagram of labyrinth of the present invention Behavioral training system.
Fig. 4 is the structural representation of the passage indicating door of labyrinth of the present invention Behavioral training system.
Fig. 5 is the labyrinth control circuit schematic diagram of labyrinth of the present invention Behavioral training system.
Fig. 6 is single circuit diagram to infrared probe of labyrinth of the present invention Behavioral training system.
Fig. 7 is the control circuit schematic diagram of the LED light emitting diode of labyrinth of the present invention Behavioral training system.
Fig. 8 is the award control circuit schematic diagram in the single chamber of labyrinth of the present invention Behavioral training system.
Fig. 9 is the PC control block diagram for software modules of labyrinth of the present invention Behavioral training system.
In the drawings, 1, labyrinth single chamber, 2, labyrinth inwall, 3, labyrinth control circuit, 4, power supply, 5, PC1 interface data capture card, 6, computer, 7, room door, 8, passage indicating door, 9, interchannel dividing plate, 10, reward point of release, 11, infrarede emitting diode, 12, three infrared receiving tubes, 13, LED light emitting diode, 14, training mode setting area, 15, reward regular setting area, 16, other optimum configurations district of system, 17, button area, 18, training result recording areas.
Embodiment
As Figure 1-Figure 4, embodiments of the present invention (for the back-shaped labyrinth of mouse); A kind of full-automatic mouse labyrinth Behavioral training system, forms primarily of room 2, labyrinth control circuit 3, power supply 4, PC1 interface data capture card 5 and computer 6 in labyrinth main body, labyrinth; It is characterized in that; Labyrinth main body is the hollow labyrinth that four labyrinth single chambers 1 form; be room 2 in labyrinth in the middle of hollow labyrinth; in labyrinth, room 2 is built-in with labyrinth control circuit 3 and power supply 4, and labyrinth control circuit 3 is connected with computer 6 by PC1 interface data capture card 5, and computer 6 is built-in with PC control software module; Labyrinth single chamber 1 has two room, road doors 7, is built-in with two road passage indicating doors 8, in the middle of two road passage indicating doors 8, arrange one piece of interchannel dividing plate 9, is provided with one nearby rewards point of release 10 at interchannel dividing plate 9; There are three infrarede emitting diodes 11 and three infrared receiving tubes 12 in labyrinth single chamber 1 indoor, directly over the passage indicating door 8 being installed on two feeder connection places respectively and reward the position of point of release about 10 two side; Described passage indicating door 8 is provided with multiple LED light emitting diode 13.
(1) labyrinth main body as shown in Figure 2, and labyrinth main body is the hollow labyrinth of four Room, and single chamber is the nothing top cuboid of high 45cm, wide 25cm, long 70cm.Indoor have two wide for 12cm passage.There are three infrarede emitting diodes, 11, three infrared receiving tubes 12 indoor, 2cm place and 7cm position before rewarding point of release 4 directly over the passage indicating door 8 not being installed on two feeder connection places.Feeder connection is equipped with passage indicating door 8, Men Gaowei 5cm.4 rooms are by back-shaped arrangement.
(2) structure of passage indicating door 8 as shown in Figure 3; Access door height is about 5cm, door is equipped with multiple LED light emitting diode installation site apart from base plate height about 3cm (close with mouse height of sighting line).
(3) list is shown in Fig. 6 to the circuit diagram of infrared probe.The transmitting terminal D3 positive pole of infrared probe connects VCC (DC5V) end of power supply, meets the ground terminal GND of power supply after negative pole series connection protective resistance R18.The receiving terminal DS3 negative pole of infrared probe connects the VCC end of power supply, and positive pole separates two lines, connects the ground terminal of power supply after a line serial resistance R17, and a line connects as analog signal input exports to data collecting card.
(4) on access door 2, control circuit such as Fig. 7 of LED light emitting diode shows.Wherein, R1, R5, R6, R19 are the resistance in 330 Europe, and L1 is white light emitting diode, and Q1 is triode.R6 mono-termination power VCC, one termination L1 positive pole, R5 and R6 is in parallel, L1 negative pole connects the colelctor electrode (c pole) of three collector Q1, the emitter stage of Q1 meets power supply GND and holds, and R1 and L1 is in parallel, and base stage is connected with R19 one end, the R19 other end is connected with the digital I/O delivery outlet of capture card, and capture card can control the light on and off of corresponding LED by the level drawing high/set low corresponding I/O mouth.
(5) award control circuit such as the Fig. 8 in single chamber shows.In figure, J1 is a magnetic valve, and U1 is relay, and D4 is an earial drainage diode.The negative pole (black) of magnetic valve connects the-12V end of power supply, positive pole (red) is connected with power supply+12V by relay, control coil one end of relay is connected with one of capture card digital I/O delivery outlet, and be connected with the negative pole of earial drainage diode D4, D4 just and crossing with the relay control coil other end after meet GND.
(6) operation principle of whole single chamber can be briefly described: trainer sets training mode and rewards probability on upper computer software.The LED light on and off on control two access doors (LED on general two passages bright quantity different) are gone in setting requirement by trainer by software.Then trainer puts into a mouse in indoor, labyrinth, mouse cross wherein an access door time, the infrared light that infrared transmitting tube that the infrared receiving tube be installed on access door makes it not receive right opposite is launched will be blocked.Corresponding PC control software program will catch the This move of mouse, and identifiable design goes out the channel number that mouse passes through, thus the LED bright light number analyzed further on this access door and the corresponding award amount under current setting, when the red isolychn that the transmitting tube that the infrared receiving diode before mouse continues to advance forward and covers award point of release receives its right opposite sends, PC control software program is caught this action of mouse and is given mouse award according to the Controlling solenoid valve that arranges of trainer, and upper computer software is by record experimental record simultaneously.A training action like this completes.Mouse enters next laboratory and carries out next training action.According to this until reach when trainer sets experiment number and stop whole experiment.It is to be noted whether PC control software the automatic decision action that goes out mouse can meet training requirement, and the action of mistake successfully will be recorded and do not given any award.
(7) circuit protecting element: because relay Inside coil is equivalent to an inductance; according to Lenz's law; if Fig. 5 (c) is when relay control signal E1 becomes low level from high level; will store certain energy in relay Inside coil, in coil, residual amount of energy does not discharge and likely will can cause the following cisco unity malfunction of relay.If draw at No. 1 mouth of relay U1 the negative pole end that a line meets a diode D4, and by the plus earth of D4.Like this, when becoming low level from high level for No. 1 of U1, relay Inside coil and D4 will form one and let out energy loop, and the energy of relay internal reservoir can successfully discharge.Thus relay can be made normally to work always.
Claims (9)
1. a full-automatic mouse labyrinth Behavioral training system, primarily of room, labyrinth control circuit, power supply, PC1 interface data capture card and computer composition in labyrinth main body, labyrinth; It is characterized in that; Labyrinth main body is the hollow labyrinth of four labyrinth single chamber compositions, be room in labyrinth in the middle of hollow labyrinth, in labyrinth, indoor are equipped with labyrinth control circuit and power supply, and labyrinth control circuit is connected with computer by PC1 interface data capture card, and computer is built-in with PC control software module.
2. one according to claim 1 full-automatic mouse labyrinth Behavioral training system, is characterized in that; Described labyrinth single chamber has two room, road doors, is built-in with two road passage indicating doors, in the middle of two road passage indicating doors, arrange one piece of interchannel dividing plate, is provided with one nearby rewards point of release at interchannel dividing plate.
3. one according to claim 1 full-automatic mouse labyrinth Behavioral training system, is characterized in that; There are three infrarede emitting diodes and three infrared receiving tubes in single chamber indoor, described labyrinth, directly over the passage indicating door being installed on two feeder connection places respectively and reward the position of two sides about point of release.
4. one according to claim 1 full-automatic mouse labyrinth Behavioral training system, is characterized in that; Described passage indicating door is provided with multiple LED light emitting diode, and the PC control software module in computer can control the light on and off of each LED light emitting diode, and passage indicating door is removable and replaceable, and access door is installed on feeder connection place by draw-in groove.
5. one according to claim 1 full-automatic mouse labyrinth Behavioral training system, is characterized in that; Described labyrinth single chamber arranges some infrared probes; Near described award point of release, award control valve is set.
6. one according to claim 1 full-automatic mouse labyrinth Behavioral training system, is characterized in that; In described labyrinth, room is rewarded and a high definition network head with erection for installing labyrinth control circuit.
7. one according to claim 1 full-automatic mouse labyrinth Behavioral training system, is characterized in that; There is training mode setting area at described its interface of PC control software module, rewards regular setting area, other optimum configurations district 15 of system, button area and training result recording areas 17.
8. one according to claim 1 full-automatic mouse labyrinth Behavioral training system, is characterized in that; Described control circuit mainly wraps the circuit of suitable infrared acquisition, the control circuit of LED light emitting diode is given into award control circuit; Wherein, single VCC (DC5V) end transmitting terminal D3 positive pole of the infrared probe of the circuit of infrared acquisition being connect to power supply, the ground terminal GND of power supply is met after negative pole series connection protective resistance R18, the receiving terminal DS3 negative pole of infrared probe connects the VCC end of power supply, positive pole separates two lines, article one, connect the ground terminal of power supply after line serial resistance R17, a line connects as analog signal input exports to data collecting card; In the control circuit of the LED light emitting diode on single access door, R1, R5, R6, R19 are the resistance in 330 Europe, and L1 is white light emitting diode, and Q1 is triode; R6 mono-termination power VCC, one termination L1 positive pole, R5 and R6 is in parallel, L1 negative pole connects the colelctor electrode (c pole) of three collector Q1, the emitter stage of Q1 meets power supply GND and holds, and R1 and L1 is in parallel, and base stage is connected with R19 one end, the R19 other end is connected with the digital I/O delivery outlet of capture card, and capture card can control the light on and off of corresponding LED by the level drawing high/set low corresponding I/O mouth; In single award control circuit figure, J1 is a magnetic valve, and U1 is relay, and D4 is an earial drainage diode; The negative pole (black) of magnetic valve connects the-12V end of power supply, positive pole (red) is connected with power supply+12V by relay, control coil one end of relay is connected with one of capture card digital I/O delivery outlet, and be connected with the negative pole of earial drainage diode D4, D4 just and crossing with the relay control coil other end after ground connection.
9. a method of operating for full-automatic mouse labyrinth according to claim 1 Behavioral training system, is characterized in that; For mouse Behavioral training, specific tasks flow process has:
(1), before task starts, programme-control is by all LED of each room and reward control valve closedown;
(2) trainer sets training mode and training relevant parameter in PC control software module;
(3) training is clicked starting in computer and is trained button and an experimental subjects is put in a laboratory wherein;
(4) ensuing training is all that system completes automatically, until the experiment number reaching trainer's setting stops, ejection training is completed prompting message window by PC control software module;
(5) whether trainer is according to the actual conditions judgment experiment result of whole experimentation effectively and whether preserve this experimental result;
(6) from experiment labyrinth, take out mouse, close computer, namely close all LED and reward control valve, completing a training experiment.
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CN108094229A (en) * | 2017-12-25 | 2018-06-01 | 南京医科大学 | A kind of Behavior test platform and method for detecting animal cooperation behavior |
CN110122366A (en) * | 2019-07-02 | 2019-08-16 | 淮阴师范学院 | A kind of learning and memory of little mouse behaviouristics training test device |
WO2022082840A1 (en) * | 2020-10-22 | 2022-04-28 | 垒途智能教科技术研究院江苏有限公司 | Brain training evaluation system based on smart maze and evaluation method therefor |
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Application publication date: 20160113 |