CN105230177A - Pretreatment method for ginkgo seed germination promotion by physical methods - Google Patents

Pretreatment method for ginkgo seed germination promotion by physical methods Download PDF

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CN105230177A
CN105230177A CN201510717219.8A CN201510717219A CN105230177A CN 105230177 A CN105230177 A CN 105230177A CN 201510717219 A CN201510717219 A CN 201510717219A CN 105230177 A CN105230177 A CN 105230177A
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seed
germination
seeds
degrees celsius
ginkgo
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CN105230177B (en
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Guangdong Maoyi Smart Ecology Co ltd
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Abstract

The invention relates to the field of germination promotion methods for ginkgo seeds. One-year-old ginkgo is low in germination percentage and long in germination time; and in order to solve the problem that ginkgo seeds are relatively long in germination time, the invention provides a pretreatment method for ginkgo seed germination promotion. In order to solve the technical problem, the technical scheme adopted by the invention is as follows: the pretreatment method for the ginkgo seed germination promotion by physical methods comprises the steps: (A) selecting the seeds, (B) carrying out low-temperature high-atmospheric-pressure storage and (C) carrying out high-temperature low-atmospheric-pressure storage. Compared with seeds without being subjected to pretreatment, the seeds pretreated by adopting the pretreatment method have the following advantages that the germination of one-year-old ginkgo seeds is promoted; the rapid germination of the seeds is promoted; the germination percentage is increased; and the seed decay ratio is lowered.

Description

Physical method urgees the preprocess method of bud to gingko seeds
The application is application number: 2013106379587, invention and created name is: physical method urgees the preprocess method of bud to gingko seeds, the applying date is: the divisional application of the application for a patent for invention on December 3rd, 2013.
Technical field
The invention belongs to agricultural field, the present invention relates to the short bud method field of gingko seeds specifically.
Background technology
Ginkgo of the present invention refers to that Classification system is the plant of GinkgobilobaL., and its seed germination time is longer, and germination rate is lower.People wish to promote its seed germination.
Summary of the invention
In order to solve Germination of Ginkgo Biloba Seeds time longer problem, and the problem that germination rate is lower, the present invention proposes the preprocess method that a kind of gingko seeds urgees bud.
The technical scheme that technical solution problem of the present invention adopts is:
Physical method urgees the preprocess method of bud to gingko seeds, the steps include:
A, seed selection: gingko seeds is placed in clear water, the seed that removing is floated, takes out and sinks to water-bed seed, then dry;
B, low temperature hyperbar is stored: the seed storage selected by step A subzero 15 degrees Celsius to subzero 20 degrees Celsius, air pressure is in 145KPa to 160KPa environment, store 120 hours,
C, high temperature atmospheric depression is stored: the seed storage by step B process is existed: 45 degrees Celsius, and air pressure is in 50KPa to 60KPa environment, and duration is 120 hours.
Embodiment
Gingko seeds collecting location: Lianyungang Area, after fruit physical maturity comes off, collects on the ground.Guarantee that gingko seeds is the seed of giving birth to then.Acquisition time: mid-October.
Various equivalent modifications should be known: after seed collection, ventilation, dry normal temperature environment should be placed on, carry out again storing (also referred to as storage) after seed drying, various equivalent modifications also should be known, drying means described here, be conventional drying methods, can not allow seed absolute part.
Seed selection mode: gingko seeds is placed in clear water, the seed that removing is floated, takes out and sinks to water-bed seed, then dry; Clear water refers to clean water, without obvious impurity, and available running water.
Low temperature of the present invention refers to: subzero 15 degrees Celsius to subzero 20 degrees Celsius.
Hyperbar of the present invention refers to: air pressure is 145KPa to 160KPa.
Low temperature hyperbar storage concrete operations mode is: seed is placed in airtight steel drum, use air compressor to squeeze into air, and the air pressure squeezed in steel drum is to a little higher than 160KPa.Various equivalent modifications should be known, and airtight steel drum has an air gauge (being also manometer) showing air pressure.Then, this airtight steel drum is put in refrigerator and stores 120 hours, the temperature of refrigerator is subzero 15 degrees Celsius to subzero 20 degrees Celsius, and due to reasons such as temperature reductions, air pressure can decline, and in the present invention, requires that barometric registration is at 145KPa to 160KPa.Various equivalent modifications utilizes The Ideal-Gas Equation to be easy to know, and the air pressure squeezed in steel drum can not be higher than 160KPa too many, because high too many, the air pressure after putting into refrigerator will be caused higher than 160KPa.
High temperature of the present invention refers to: 45 degrees Celsius.
Atmospheric depression of the present invention refers to: air pressure is 50KPa to 60KPa.
High temperature atmospheric depression storage concrete operations mode is: seed is loaded in steel drum, and use air exhauster exhaust, until internal gas pressure is about 50KPa, then, this airtight steel drum is put into heater, and heater is the insulating box that upper seascape steps the commodity " DHG series electrothermostat 303-0A " by name that instrument and equipment Co., Ltd produces, and regulates temperature to be 45 degrees Celsius, and under 45 degrees Celsius of environment, the air pressure in airtight steel drum is 50KPa to 60KPa.
Of the present invention bleed reduce air pressure, inflation increases air pressure and all adopts prior art to complete: the time of experience of bleeding is about 5 minutes, and the time of inflation experience is about 2 minutes, makes steel drum air pressure return to the atmospheric pressure time used and is about 1 minute.Various equivalent modifications should know the implication of " about " word herein.
What need explanation also has: the fluctuation of refrigerator cryogenic temperature is comparatively large usually, and the change of subzero 15 degrees Celsius to subzero 20 degrees Celsius belongs to normal excursion.Because gas pressure intensity temperature influence is comparatively large, so air pressure of the present invention is that a scope also belongs to normal.
Next, provide the experimental data of the method for the invention and the data of comparative trial, need to introduce:
1, the time outside test (low temperature, high pressure, high temperature, low pressure and combination thereof), the storage method of seed is usual manner: drying, ventilation, normal temperature.The storage of seed under dry, ventilation, normal temperature belongs to prior art, and be the technology that various equivalent modifications is known, such statement clearly states.In the present invention, the room temperature storage temperature of seed is: 5 degrees Celsius to 20 degrees Celsius.
2, sprouting environment is: the illumination box of employing is that Shanghai Sheng Ke instrument and equipment Co., Ltd produces, marque is GZX-400BS-III.Sprouting environment is: in evenly distributed for the seed culture dish in being covered with filter paper, cover one deck filter paper again above seed, spray distilled water with watering can, the cultivation temperature on daytime is 35 degrees Celsius, the cultivation temperature at night is 30 degrees Celsius, and relative moisture is 65 to percent 85 percent.Intensity of illumination 15000 lux (daytime, night all provide illumination), (intensity of illumination to refer in unit are accept the energy of visible ray, be called for short illumination, unit lux (Lux or Lx)), daily observation also keeps filter paper moistening.
Why adopting the Germination Temperature of 35 degrees Celsius to 30 degrees Celsius, is generally adopt this temperature to impel the sprouting of gingko seeds because of vast breeding, nursery worker.
3, the computational methods of germination rate: the computational methods illustrating germination rate: one group of seed amount 100, germinate 40, then use 40 divided by 100, result represents to be exactly 40 percent with percentage, and germination rate is 40 percent.
4, the time of this test process seed is: in November,------December carries out, and the time of Seed Germination Test is the January of December------next year.That is, plucking from seed, being about 4 months to completing the maximum duration sprouting test.
5, the statistical method of rotten kind of rate: sprouting test, after 16 days, is got the seed not having to sprout, made an inventory of rotten kind of quantity after destroying shell, then, with this group seed sum of rotten kind of number ratio in this group seed, is scaled percentage, just obtains rotten kind of rate.Gingko seeds, when sprouting rotten kind of environment, becomes pulpous state in shell, foul smelling.
Sprouting test result is as follows:
1, adopt the seed of the method for the invention process:
First group: the seed adopting the method for the invention process, usual manner does sprouting test after preserving 1 week, often organize 100,4 days germination rates percent 20,9 days germination rates percent 89, rotten kind of rate percent 6.
Second group: the seed adopting the method for the invention process, usual manner does sprouting test after preserving 2 weeks, often organize 100,4 days germination rates percent 21,9 days germination rates percent 88, rotten kind of rate percent 7.
First group: the seed adopting the method for the invention process, usual manner does sprouting test after preserving 3 weeks, often organize 100,4 days germination rates percent 22,9 days germination rates percent 88, rotten kind of rate percent 6.
Second group: the seed adopting the method for the invention process, usual manner does sprouting test after preserving 4 weeks, often organize 100,4 days germination rates percent 21,9 days germination rates percent 89, rotten kind of rate percent 7.
The method of the invention is:
A, seed selection: gingko seeds is placed in clear water, the seed that removing is floated, takes out and sinks to water-bed seed, then dry; What various equivalent modifications knows is " drying ".The method of drying that the present invention adopts belongs to prior art: spread out and be placed on ventilation, temperature about 25 degrees Celsius, dry in the air 2---3 hour naturally.
B, low temperature hyperbar is stored: the seed storage selected by step A subzero 15 degrees Celsius to subzero 20 degrees Celsius, air pressure is in 145KPa to 160KPa environment, store 120 hours,
C, high temperature atmospheric depression is stored: the seed storage by step B process is existed: 45 degrees Celsius, and air pressure is in 50KPa to 60KPa environment, and duration is 120 hours.
From collection seed to carry out the storage of low temperature hyperbar and last 6 weeks, from carry out low temperature hyperbar and be stored to and start to carry out high temperature atmospheric depression and store and last 2 weeks.
2, without the pretreated seed of any link, seed preserves 3 months in a conventional manner, sprouts test result to be: often organize 100,4 days germination rates percent 0,9 days germination rates percent 13,15 days germination rates percent 34, rotten kind of rate percent 34.
3, low temperature is only adopted (to store 120 hours to subzero 20 degrees Celsius for subzero 15 degrees Celsius,) pretreatment, air pressure is an atmospheric pressure, without high temperature treatment, sprouting test is done after preserving 4 weeks in the usual way after process, test situation is: often organize 100,4 days germination rates percent 0,9 days germination rates percent 12,15 days germination rates percent 39, rotten kind of rate percent 31.
4, processing method is low temperature and hyperbar (subzero 15 degrees Celsius to subzero 20 degrees Celsius, air pressure is in 145KPa to 160KPa environment, store 120 hours), sprouting test is done after preserving 4 weeks in the usual way after process, test situation is: often organize 100,4 days germination rates percent 3,9 days germination rates percent 21,15 days germination rates percent 41, rotten kind of rate percent 27.
5, only adopt high temperature (45 degrees Celsius, and duration is 120 hours) process, air pressure is an atmospheric pressure, does sprouting test after preserving 4 weeks in the usual way after process, and test situation is: often organize 100,4 days germination rates percent 0,9 days germination rates percent 13,15 days germination rates percent 42, rotten kind of rate percent 32.
6, first adopt low temperature and hyperbar (subzero 15 degrees Celsius to subzero 20 degrees Celsius, air pressure is in 145KPa to 160KPa environment, store 120 hours), rear employing high temperature storage (45 degrees Celsius, and duration is 120 hours, air pressure is an atmospheric pressure) mode process, sprouting test is done after preserving 4 weeks in the usual way after process, test situation is: often organize 100,4 days germination rates percent 4,9 days germination rates percent 21,15 days germination rates percent 38, rotten kind of rate percent 22.
7, (air pressure is 50KPa to 60KPa only to adopt atmospheric depression storage, and duration is 120 hours, temperature is 5 degrees Celsius to 20 degrees Celsius), do sprouting test after preserving 4 weeks in the usual way after process, test situation is: often organize 100,4 days germination rates percent 2,9 days germination rates percent 13,15 days germination rates 42, rotten kind of rate percent 33.
The atmospheric pressure of this specification indication, allows to fluctuate within the specific limits, because different regions atmospheric pressure is different, the different time atmospheric pressure of areal is also different, so the atmospheric pressure of indication allows to fluctuate within the specific limits here.In other words, the atmospheric pressure of this specification indication is: the pressure of air, is about the pressure that 76cm mercury column produces.
Adopt the seed that the present invention is pretreated, compare without this pretreated, have the following advantages: 1, promote the sprouting of raw gingko seeds then, 2, promote the fast-germination of seed, 3, improve germination rate, 4, reduce rotten kind of rate.

Claims (1)

1. physical method urgees the preprocess method of bud to gingko seeds, the steps include:
A, seed selection: gingko seeds is placed in clear water, the seed that removing is floated, takes out and sinks to water-bed seed, then dry;
B, low temperature hyperbar is stored: the seed storage selected by step A subzero 15 degrees Celsius to subzero 20 degrees Celsius, air pressure is in 145KPa to 160KPa environment, store 120 hours,
C, high temperature atmospheric depression is stored: the seed storage by step B process is existed: 45 degrees Celsius, and air pressure is in 50KPa to 60KPa environment, and duration is approximately 120 hours.
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CN104012210A (en) * 2014-06-28 2014-09-03 崔涵宇 Sprouting method for gingko seeds
CN104126353A (en) * 2014-07-18 2014-11-05 山东农业大学 Method for sowing ginkgo biloba seed with testa of ancient tree

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WO2010132822A1 (en) * 2009-05-14 2010-11-18 Product Partners, Llc Nutritional compositions for reducing oxidative damage
CN102165883A (en) * 2010-02-25 2011-08-31 上海信谊百路达药业有限公司 Seeding and seedling growing method of gingkoes
CN102577697A (en) * 2012-03-01 2012-07-18 玉溪明珠花卉股份有限公司 Oriental lily seed variable-temperature treatment method

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CN101828447A (en) * 2009-03-13 2010-09-15 章桂明 Method for treating plant seed
CN102986332B (en) * 2012-12-07 2013-12-25 浙江农林大学 Fast large-scale germination accelerating method for palm seed

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WO2010132822A1 (en) * 2009-05-14 2010-11-18 Product Partners, Llc Nutritional compositions for reducing oxidative damage
CN102165883A (en) * 2010-02-25 2011-08-31 上海信谊百路达药业有限公司 Seeding and seedling growing method of gingkoes
CN102577697A (en) * 2012-03-01 2012-07-18 玉溪明珠花卉股份有限公司 Oriental lily seed variable-temperature treatment method

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