CN103636311B - Pretreatment method for accelerating germination of xanthium sibiricum seeds by physical method - Google Patents
Pretreatment method for accelerating germination of xanthium sibiricum seeds by physical method Download PDFInfo
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- CN103636311B CN103636311B CN201310637959.1A CN201310637959A CN103636311B CN 103636311 B CN103636311 B CN 103636311B CN 201310637959 A CN201310637959 A CN 201310637959A CN 103636311 B CN103636311 B CN 103636311B
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- 241001251949 Xanthium sibiricum Species 0.000 title claims abstract description 30
- 238000000053 physical method Methods 0.000 title claims description 4
- 230000035784 germination Effects 0.000 title abstract description 33
- 238000002203 pretreatment Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 claims abstract description 30
- 230000008569 process Effects 0.000 claims description 16
- 108010016634 Seed Storage Proteins Proteins 0.000 claims description 6
- 238000012360 testing method Methods 0.000 description 20
- 229910000831 Steel Inorganic materials 0.000 description 9
- 239000010959 steel Substances 0.000 description 9
- 238000012986 modification Methods 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000005286 illumination Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000007226 seed germination Effects 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 238000000205 computational method Methods 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
The invention relates to the field of germination accelerating methods for xanthium sibiricum seeds. Annual xanthium sibiricum is difficult to germinate under a low-temperature environment. The invention provides a pretreatment method for accelerating germination of the xanthium sibiricum seeds in order to solve the problems that the xanthium sibiricum seeds are low in germination speed and the annual seeds are difficult to germinate under low temperature. According to the technical scheme, the pretreatment method for accelerating germination of the xanthium sibiricum seeds comprises the steps of A, seed selection; B, high-air-pressure storage at low temperature; and C, low-air-pressure storage at high temperature. Compared with seeds which are not pretreated, the seeds pretreated by the pretreatment method disclosed by the invention have the advantages that 1, the germination of the annual xanthium sibiricum seeds is accelerated, and 2, the germination under low temperature is accelerated.
Description
Technical field
The invention belongs to agricultural field, the present invention relates to the short bud method field of Siberian cocklebur seed specifically.
Background technology
Siberian cocklebur of the present invention refers to that Latin is called the plant of Xanthium sibiricum Patrin ex Widder, its seed germination time is longer, along with scientific worker deepens further to the research of Siberian cocklebur, people thirst for the sprouting time shortening Siberian cocklebur seed, especially wish that the Siberian cocklebur seed of giving birth to then can fast germination.In addition, Siberian cocklebur seed germinates very slow at a lower temperature, and grower wishes that Siberian cocklebur seed also can be sprouted with the shorter time at a lower temperature.Seed of the present invention just refers to the seed of Siberian cocklebur.The optimum Germination Temperature of Siberian cocklebur seed is near 20 degrees Celsius, but the temperature span of natural environment is comparatively large, and grower wishes that Siberian cocklebur seed is sprouted early.
Summary of the invention
In order to solve Siberian cocklebur seed germination time longer problem, seed raw especially then sprouts the problem of difficulty at a lower temperature, and the present invention proposes the preprocess method that a kind of Siberian cocklebur seed urgees bud.
The technical scheme that technical solution problem of the present invention adopts is:
Physical method urgees the preprocess method of bud to Siberian cocklebur seed, the steps include:
A, seed selection: select the Siberian cocklebur seed that kind of grain is evenly full,
B, low temperature hyperbar is stored: the seed storage selected by step A subzero 15 degrees Celsius to subzero 20 degrees Celsius, air pressure is in 130KPa to 140KPa environment, store 120 hours,
C, high temperature atmospheric depression is stored: the seed storage by step B process is existed: 45 degrees Celsius, and air pressure is in 50KPa to 60KPa environment, and duration is 120 hours.
Embodiment
Siberian cocklebur seed collection place: field, Hami area, takes artificial mode of plucking, can guarantee that Siberian cocklebur seed is the seed of giving birth to then.Plucking time: early October.Various equivalent modifications should be known: after seed collection, ventilation, dry normal temperature environment should be placed on, carry out again storing (also referred to as storage) after seed drying, various equivalent modifications also should be known, drying means described here, be conventional drying methods, can not allow seed absolute part.
Seed selection mode: hand picking, removes stone, weeds, selects the Siberian cocklebur seed that kind of grain is evenly full.Plant the grain evenly full technology belonging to various equivalent modifications and know, so, plant grain and evenly clearly state full belonging to.
Low temperature of the present invention refers to: subzero 15 degrees Celsius to subzero 20 degrees Celsius.
Hyperbar of the present invention refers to: air pressure is 130KPa to 140KPa.
Low temperature hyperbar storage concrete operations mode is: seed is placed in airtight steel drum, use air compressor to squeeze into air, and the air pressure squeezed in steel drum is to a little higher than 140KPa.Various equivalent modifications should be known, and airtight steel drum has an air gauge (being also manometer) showing air pressure.Then, this airtight steel drum is put in refrigerator and stores 120 hours, the temperature of refrigerator is subzero 15 degrees Celsius to subzero 20 degrees Celsius, and due to reasons such as temperature reductions, air pressure can decline, and in the present invention, requires that barometric registration is at 130KPa to 140KPa.Various equivalent modifications utilizes The Ideal-Gas Equation to be easy to know, and the air pressure squeezed in steel drum can not be higher than 140KPa too many, because high too many, the air pressure after putting into refrigerator will be caused higher than 140KPa.
High temperature of the present invention refers to: 45 degrees Celsius.
Atmospheric depression of the present invention refers to: air pressure is 50KPa to 60KPa.
High temperature atmospheric depression storage concrete operations mode is: seed is loaded in steel drum, and use air exhauster exhaust, until internal gas pressure is about 50KPa, then, this airtight steel drum is put into heater, and heater is the insulating box that upper seascape steps the commodity " DHG series electrothermostat 303-0A " by name that instrument and equipment Co., Ltd produces, and regulates temperature to be 45 degrees Celsius, and under 45 degrees Celsius of environment, the air pressure in airtight steel drum is 50KPa to 60KPa.
Of the present invention bleed reduce air pressure, inflation increases air pressure and all adopts prior art to complete: the time of experience of bleeding is about 5 minutes, and the time of inflation experience is about 2 minutes, makes steel drum air pressure return to the atmospheric pressure time used and is about 1 minute.Various equivalent modifications should know the implication of " about " word herein.
What need explanation also has: the fluctuation of refrigerator cryogenic temperature is comparatively large usually, and the change of subzero 15 degrees Celsius to subzero 20 degrees Celsius belongs to normal excursion.Because gas pressure intensity temperature influence is comparatively large, so air pressure of the present invention is that a scope also belongs to normal.
Next, provide the experimental data of the method for the invention and the data of comparative trial, need to introduce:
1, the time outside test (low temperature, high pressure, high temperature, low pressure and combination thereof), the storage method of seed is usual manner: drying, ventilation, normal temperature.The storage of seed under dry, ventilation, normal temperature belongs to prior art, and be the technology that various equivalent modifications is known, such statement clearly states.In the present invention, the room temperature storage temperature of seed is: 5 degrees Celsius to 20 degrees Celsius.
2, sprouting environment is: the illumination box of employing is that Shanghai Sheng Ke instrument and equipment Co., Ltd produces, marque is GZX-400BS-III.Sprouting environment is: in evenly distributed for the seed culture dish in being covered with filter paper, cover one deck filter paper again above seed, spray distilled water with watering can, the cultivation temperature on daytime is 15 degrees Celsius, the cultivation temperature at night is 12 degrees Celsius, and relative moisture is 65 to percent 85 percent.Intensity of illumination 15000 lux (daytime, night all provide illumination), (intensity of illumination to refer in unit are accept the energy of visible ray, be called for short illumination, unit lux (Lux or Lx)), daily observation also keeps filter paper moistening.This sprouting environmental temperature is obviously lower than suitable Germination Temperature.
3, the computational methods of germination rate: the computational methods illustrating germination rate: one group of seed amount 100, germinate 40, then use 40 divided by 100, result represents to be exactly 40 percent with percentage, and germination rate is 40 percent.
4, the time of this test process seed is: in November,------December carries out, and the time of Seed Germination Test is the January of December------next year.That is, plucking from seed, being about 4 months to completing the maximum duration sprouting test.
Sprouting test result is as follows:
1, adopt the seed of the method for the invention process:
First group: the seed adopting the method for the invention process, usual manner does sprouting test after preserving 1 week, often organize 100,5 days germination rates percent 26,11 days germination rates percent 91.
Second group: the seed adopting the method for the invention process, usual manner does sprouting test after preserving 2 weeks, often organize 100,5 days germination rates percent 27,11 days germination rates percent 91.
First group: the seed adopting the method for the invention process, usual manner does sprouting test after preserving 3 weeks, often organize 100,5 days germination rates percent 26,11 days germination rates percent 92.
Second group: the seed adopting the method for the invention process, usual manner does sprouting test after preserving 4 weeks, often organize 100,5 days germination rates percent 25,11 days germination rates percent 91.
The method of the invention is:
A, seed selection: select the Siberian cocklebur seed that kind of grain is evenly full,
B, low temperature hyperbar is stored: the seed storage selected by step A subzero 15 degrees Celsius to subzero 20 degrees Celsius, air pressure is in 130KPa to 140KPa environment, store 120 hours,
C, high temperature atmospheric depression is stored: the seed storage by step B process is existed: 45 degrees Celsius, and air pressure is in 50KPa to 60KPa environment, and duration is 120 hours.
From collection seed to carry out the storage of low temperature hyperbar and last 6 weeks, from carry out low temperature hyperbar and be stored to and start to carry out high temperature atmospheric depression and store and last 2 weeks.
2, without the pretreated seed of any link, seed preserves 3 months in a conventional manner, sprouts test result to be: often organize 100,5 days germination rates percent 0,11 days germination rates percent 2.
3, low temperature is only adopted (to store 120 hours to subzero 20 degrees Celsius for subzero 15 degrees Celsius,) pretreatment, air pressure is an atmospheric pressure, without high temperature treatment, do sprouting test after preserving 4 weeks in the usual way after process, test situation is: often organize 100,5 days germination rates percent 0,11 days germination rates percent 2.
4, processing method is low temperature and hyperbar (subzero 15 degrees Celsius to subzero 20 degrees Celsius, air pressure is in 130KPa to 140KPa environment, store 120 hours), sprouting test is done after preserving 4 weeks in the usual way after process, test situation is: often organize 100,5 days germination rates percent 6,11 days germination rates percent 19.
5, only adopt high temperature (45 degrees Celsius, and duration is 120 hours) process, air pressure is an atmospheric pressure, does sprouting test after preserving 4 weeks in the usual way after process, and test situation is: often organize 100,5 days germination rates percent 6,11 days germination rates percent 20.
6, first adopt low temperature and hyperbar (subzero 15 degrees Celsius to subzero 20 degrees Celsius, air pressure is in 130KPa to 140KPa environment, store 120 hours), the mode process of rear employing high temperature storage (45 degrees Celsius, and duration is 120 hours, air pressure is an atmospheric pressure), sprouting test is done after preserving 4 weeks in the usual way after process, test situation is: often organize 100,5 days germination rates percent 13,11 days germination rates percent 42.
7, (air pressure is 50KPa to 60KPa, and duration is 120 hours only to adopt atmospheric depression storage, temperature is 5 degrees Celsius to 20 degrees Celsius), do sprouting test after preserving 4 weeks in the usual way after process, test situation is: often organize 100,5 days germination rates percent 7,11 days germination rates percent 22.
The atmospheric pressure of this specification indication, allows to fluctuate within the specific limits, because different regions atmospheric pressure is different, the different time atmospheric pressure of areal is also different, so the atmospheric pressure of indication allows to fluctuate within the specific limits here.In other words, the atmospheric pressure of this specification indication is: the pressure of air, is about the pressure that 76cm mercury column produces.
Adopt the seed that the present invention is pretreated, compare without pretreated, have the following advantages: 1, promote the sprouting of raw Siberian cocklebur seed then, 2, promote the sprouting under lower temperature.
Claims (1)
1. physical method urgees the preprocess method of bud to Siberian cocklebur seed, the steps include:
A, seed selection: select the Siberian cocklebur seed that kind of grain is evenly full,
B, low temperature hyperbar is stored: the seed storage selected by step A subzero 15 degrees Celsius, air pressure is in 130KPa to 140KPa environment, store 120 hours,
C, high temperature atmospheric depression is stored: the seed storage by step B process is existed: 45 degrees Celsius, and air pressure is in 50KPa to 60KPa environment, and duration is 120 hours.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310637959.1A CN103636311B (en) | 2013-12-03 | 2013-12-03 | Pretreatment method for accelerating germination of xanthium sibiricum seeds by physical method |
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| CN201310637959.1A CN103636311B (en) | 2013-12-03 | 2013-12-03 | Pretreatment method for accelerating germination of xanthium sibiricum seeds by physical method |
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| CN103636311B true CN103636311B (en) | 2015-07-01 |
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| FR2710812B1 (en) * | 1993-10-04 | 1995-12-29 | Goemar Lab Sa | Method and installation for the treatment of seeds and bulbs. |
| CN101828447A (en) * | 2009-03-13 | 2010-09-15 | 章桂明 | Method for treating plant seed |
| CN102714943A (en) * | 2012-06-06 | 2012-10-10 | 大连斯扶特农业科技有限公司 | Method for breaking dormancy of seeds of panax notoginseng |
| CN102986332B (en) * | 2012-12-07 | 2013-12-25 | 浙江农林大学 | Fast large-scale germination accelerating method for palm seed |
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