CN105223249A - A kind of preparation method of the chemical sensor for thrombocytin detection - Google Patents
A kind of preparation method of the chemical sensor for thrombocytin detection Download PDFInfo
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- CN105223249A CN105223249A CN201510667754.7A CN201510667754A CN105223249A CN 105223249 A CN105223249 A CN 105223249A CN 201510667754 A CN201510667754 A CN 201510667754A CN 105223249 A CN105223249 A CN 105223249A
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Abstract
For the preparation method of chemical sensor that thrombocytin detects, comprise the preparation of conducting polymer modified electrode: first glass-carbon electrode is carried out ultrasonic cleaning in redistilled water after polishing; Then the glass-carbon electrode after cleaning puts into M polymer monomer, the water-soluble quinones sulfonate of M and graphene oxide solution, logical nitrogen is after 30 minutes, three-electrode system is adopted to carry out continuous current electro-deposition, then gained modified electrode is carried out electrochemical reduction, obtain conducting polymer/quinones sulfonated bodies/graphene oxide composite material modified electrode; Second step: the preparation of conducting polymer electrochemical sensor that noble metal nano particles is modified: described sensor is the conducting polymer electrochemical sensor that a kind of noble metal nano particles is modified, the quick detection that this sensor can realize thrombocytin and Metabolites Concentration thereof with easy steps.
Description
Technical field
What the present invention relates to is device of a kind of technical field of electrochemical detection and preparation method thereof, specifically a kind of chemical sensor preparation method detected for thrombocytin.
Background technology
Thrombocytin and serotonin, be present in the argentaffin of mast cell, blood platelet and intestinal mucosa.Research shows that appendix is inflamed and causes mast cell, basocyte retting conditions, and inflammatory mediator such as release histamine, serotonin etc., in blood, the concentration of thrombocytin increases.Thrombocytin under the effect of monoamine oxidase in vivo rapid metabolization generate 5-hydroxyindoleacetic acid, get rid of from urine.Therefore the mensuration of serotonin and metabolic product 5-hydroxyindoleacetic acid content thereof has some reference value to typhlitic diagnosis.
There is at present multiple detection technique for the mensuration of serotonin and 5-hydroxyindoleacetic acid, as photometry, fluorescence method, chemoluminescence method, polarography, voltammetry and Capillary Electrophoresis etc.Although said method is for the detection of serotonin and 5-hydroxyindoleacetic acid in biological and drug sample, much method also exists pre-service complexity, cost is high, sensitivity is low and can not on same biological sample these compounds of Simultaneously test shortcoming and have impact on the application of these methods.
Summary of the invention
The present invention is directed to prior art above shortcomings, a kind of chemical sensor preparation method detected for thrombocytin and metabolic product thereof is provided.
For a preparation method for the chemical sensor that thrombocytin detects, it is characterized in that, comprise the following steps:
The first step: the preparation of conducting polymer modified electrode:
First glass-carbon electrode is carried out ultrasonic cleaning in redistilled water after polishing; Then the glass-carbon electrode after cleaning puts into M polymer monomer, the water-soluble quinones sulfonate of M and graphene oxide solution, logical nitrogen is after 30 minutes, three-electrode system is adopted to carry out continuous current electro-deposition, then gained modified electrode is carried out electrochemical reduction, obtain conducting polymer/quinones sulfonated bodies/graphene oxide composite material modified electrode;
Second step: the preparation of the conducting polymer electrochemical sensor that noble metal nano particles is modified:
Conducting polymer modified electrode is placed in the salt solusion containing solvable row noble metal N, the conducting polymer modified electrode through overscanning is taken out after carrying out scan round process, rinse several times in turn successively with absolute ethyl alcohol and redistilled water, obtain the conducting polymer electrochemical sensor that noble metal nano particles is modified.
Described quinones sulfonate is the one of anthraquinone or phenanthrenequione.
Described M polymer monomer is the one in pyrroles, aniline, 3,4-rthylene dioxythiophene.
Described noble metal N is the one in platinum, gold or silver.
Specific embodiment
Embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1:
The glass-carbon electrode that pre-service is good is put into the phosphate buffer solution of pH=7.0, in the potential range of-0.2V ~+2.0V, carry out scan round process 10min with the sweep speed of 20mV/s.
Then electrode is put into the pyrrole monomer containing 0.1M, 0.0005M phenanthrenequione sulfonate, 0.5mg/ml graphene oxide water solution, logical N
2after 30min, adopt three-electrode system to carry out continuous current electro-deposition, applying electric current is 0.5mAcm
-2, the electricity of electro-deposition is 1.2 coulombs; Electrochemical reduction is carried out being placed in three-electrode system after being cleaned by obtained polypyrrole film, applying voltage is 1.1V, electrolytic solution is PBS buffer solution, repeatedly cleans can obtain polypyrrole/phenanthrenequione sulfonate/graphene composite material modified electrode after reaction with water;
Above-mentioned modified electrode is placed in containing 1.0 × 10
-3the K of mol/L
2ptCl
6with in the KCl solution of 0.1mol/L, in the potential range of-0.4V ~+0.3V, carry out scan round process 12 with the sweep speed of 0.1V/s and enclose, namely obtain the conducting polymer electrochemical sensor that nano platinum particle is modified.
Embodiment 2:
The glass-carbon electrode that pre-service is good is put into the phosphate buffered solution of pH=5.0, in the potential range of-0.2V ~+1.0V, carry out scan round process 15min with the sweep speed of 15mV/s;
Then electrode is put into pyrrole monomer, 0.0005M anthraquinone sulphonates, the 1.0mg/ml graphene oxide water solution containing 0.2M, logical N
2after 30min, adopt three-electrode system to carry out continuous current electro-deposition, applying electric current is 2mAcm
-2, the electricity of electro-deposition is 2.0 coulombs; Electrochemical reduction is carried out being placed in three-electrode system after being cleaned by obtained polypyrrole film, applying voltage is 1.2V, electrolytic solution is PBS buffer solution, repeatedly cleans can obtain polypyrrole/anthraquinone sulphonates/graphene composite material modified electrode after reaction with water;
Above-mentioned modified electrode is placed in containing 2.5 × 10
-3the K of mol/L
2ptCl
6with in the KCl solution of 0.1mol/L, in the potential range of-0.4V ~+0.3V, carry out scan round process 16 with the sweep speed of 0.1V/s and enclose, namely obtain the conducting polymer electrochemical sensor that nano platinum particle is modified.
Embodiment 3:
The glass-carbon electrode that pre-service is good is put into the phosphate buffered solution of pH=7.0, in the potential range of-0.2V ~+2.0V, carry out scan round process 50min with the sweep speed of 20mV/s;
Then electrode is put into containing aniline monomer, 0.1M phenanthrenequione sulfonate, the 1.0mg/ml graphene oxide water solution containing 0.05M, logical N
2after 30min, adopt three-electrode system to carry out continuous current electro-deposition, applying electric current is 1mAcm
-2, the electricity of electro-deposition is 1.6 coulombs; Electrochemical reduction is carried out being placed in three-electrode system after being cleaned by obtained polyaniline film, applying voltage is 1.0V, electrolytic solution is PBS buffer solution, repeatedly cleans can obtain polyaniline/phenanthrenequione sulfonate/graphene composite material modified electrode after reaction with water;
Above-mentioned modified electrode is placed in containing 4.5 × 10
-3the K of mol/L
2ptCl
6with in the KCl solution of 0.1mol/L, in the potential range of-0.4V ~+0.3V, carry out scan round process 18 with the sweep speed of 0.1V/s and enclose, namely obtain the conducting polymer electrochemical sensor that nano platinum particle is modified.
Claims (4)
1., for a preparation method for the chemical sensor of thrombocytin detection, it is characterized in that, comprise the following steps:
The first step: the preparation of conducting polymer modified electrode:
First glass-carbon electrode is carried out ultrasonic cleaning in redistilled water after polishing; Then the glass-carbon electrode after cleaning puts into M polymer monomer, the water-soluble quinones sulfonate of M and graphene oxide solution, logical nitrogen is after 30 minutes, three-electrode system is adopted to carry out continuous current electro-deposition, then gained modified electrode is carried out electrochemical reduction, obtain conducting polymer/quinones sulfonated bodies/graphene oxide composite material modified electrode;
Second step: the preparation of the conducting polymer electrochemical sensor that noble metal nano particles is modified:
Conducting polymer modified electrode is placed in the salt solusion containing solvable row noble metal N, the conducting polymer modified electrode through overscanning is taken out after carrying out scan round process, rinse several times in turn successively with absolute ethyl alcohol and redistilled water, obtain the conducting polymer electrochemical sensor that noble metal nano particles is modified.
2. a kind of preparation method of chemical sensor detected for thrombocytin according to claim 1, it is characterized in that, described quinones sulfonate is the one of anthraquinone or phenanthrenequione.
3. a kind of preparation method of chemical sensor detected for thrombocytin according to claim 1, it is characterized in that, described M polymer monomer is the one in pyrroles, aniline, 3,4-rthylene dioxythiophene.
4. a kind of preparation method of chemical sensor detected for thrombocytin according to claim 1, it is characterized in that, described noble metal N is the one in platinum, gold or silver.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107501528A (en) * | 2017-09-06 | 2017-12-22 | 烟台智本知识产权运营管理有限公司 | The preparation of poly- (the 5 aldehyde radical indoles) nano composite material of graphene |
| CN110596218A (en) * | 2019-10-09 | 2019-12-20 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of enzyme sensor for detecting organophosphorus pesticide, product and application thereof |
| CN111054446A (en) * | 2019-12-28 | 2020-04-24 | 西安建筑科技大学 | Anthraquinone sulfonate-polyethylene dioxythiophene-indium zinc sulfide composite membrane electrode and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107501528A (en) * | 2017-09-06 | 2017-12-22 | 烟台智本知识产权运营管理有限公司 | The preparation of poly- (the 5 aldehyde radical indoles) nano composite material of graphene |
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| CN111054446A (en) * | 2019-12-28 | 2020-04-24 | 西安建筑科技大学 | Anthraquinone sulfonate-polyethylene dioxythiophene-indium zinc sulfide composite membrane electrode and preparation method thereof |
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