CN105211874B - Composite plant ferment containing probiotics and preparation method thereof - Google Patents
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Abstract
The invention discloses a kind of composite plant ferment containing probiotics and preparation method thereof, the composite plant ferment containing probiotics is prepared from by the raw material of following weight parts：30 50 parts of rice sprout, 30 50 parts of mulberry leaf, 30 50 parts of hawthorn, 30 50 parts of duck wheat, 30 50 parts of balloonflower root, 30 50 parts of reed rhizome, 30 50 parts of Buddha's hand, 30 50 parts of almond, 30 50 parts of radix glycyrrhizae, 30 50 parts of dried orange peel, 10 30 parts of Poria cocos, 30 50 parts of lily, 30 50 parts of purple perilla; 10 20 parts of enzyme live keeping agent, prebiotic 150 200 parts of bacterium solution.After enzyme live keeping agent; the activity of ferment is protected; various extracellular ferment are complete, energetic, and beneficial viable bacteria can largely pass through stomach; it is colonized in enteron aisle; amount reproduction, produces various ferment, effectively decomposes the food in enteron aisle and removes enteron aisle rubbish; maintain the beneficial microbe colony of enteron aisle, the absorption beneficial to enteron aisle to various nutrition.
Description
Technical field
The present invention relates to nutraceutical field, and in particular to a kind of composite plant ferment and its preparation side containing probiotics
Method.
Background technology
Ferment is also known as the catalyst that " enzyme " is various biochemical reactions, and ferment is present in all living cells, is it
The vigor of cell is have activated, cell is shown a variety of signs of life, U.S. naturopathy doctor henry Bert Sheng Tinuo is said：People
Body image bulb, ferment image current.Having the bulb after being powered only just can be bright, without electricity, what we had be one will not be bright
Bulb.Therefore, ferment is the indispensable important substance of human body.Ferment is divided into two major classes, external ferment and internal ferment,
Ferment in human body is limited, and when the effect of internal ferment is weak or reduces, disease will be following, therefore human body should be closed
Reason from external supplement ferment come the need for meeting metabolism, the invasion of resist the disease.
Ferment be it is so important, it is closely bound up with life, but people rhythm of life is accelerated now, residing Environment pollution
Seriously, most of people are under the state of inferior health, and add diet does not focus on causing the enzyme of missing in human body increasingly
It is many.We must absorb more ferment from meals, and food makes food again after 54 DEG C of high temperature above or finishing
In ferment destroyed.In order to supplement the ferment of needed by human body, ferment technology is developed, and ferment technology refers to multi-cultur es and is combined
The technology of type micropopulation fermentation, is a new new and high technology, traditional product such as pickles, thick broad-bean sauce, natto, miso
Etc. being referred to as ferment product.Refer in particular to, using plants such as fresh vegetables, fruit as raw material, produce through a variety of probiotics fermentions herein
Raw, the microorganism formulation containing abundant enzyme, vitamin, mineral matter and secondary metabolite.It is common water fruits and vegetables, rough
Rice, mushroom class, integration of drinking and medicinal herbs Chinese medicine etc. can serve as the raw material of ferment fermentation.Protease, lipase and superoxide dismutase
Etc. the primary efficacy enzyme for being enzyme microb class health food.
To make enzyme food more conform to modern to the diversity of nutritional need, balance and being easy to absorbability, no matter
It is that traditional handicraft or modern crafts still need further improvement.Although also occurring in that the guarantor of some addition ferment on domestic market
Health food, but it is also seldom in the application of other field of food, still there is wide development space.In consideration of it, enzyme food is either
The necessity that application of the production technology still in food all makes further progress.Therefore, develop a kind of compound containing probiotics
The manufacture craft and product of plant enzyme are always new problem anxious to be resolved.
The content of the invention
For above shortcomings in the prior art, the technical problems to be solved by the invention are to provide a kind of containing prebiotic
Composite plant ferment of bacterium and preparation method thereof.
The present invention seeks to what is be achieved through the following technical solutions：
A kind of composite plant ferment containing probiotics, is prepared from by the raw material of following weight parts：
30-50 parts of rice sprout, 30-50 parts of mulberry leaf, 30-50 parts of hawthorn, 30-50 parts of duck wheat, 30-50 parts of balloonflower root, reed rhizome 30-
50 parts, 30-50 parts of Buddha's hand, 30-50 parts of almond, 30-50 parts of radix glycyrrhizae, 30-50 parts of dried orange peel, 10-30 parts of Poria cocos, 30-50 parts of lily,
30-50 parts of purple perilla, 10-20 parts of enzyme live keeping agent, prebiotic bacterium solution 150-200 parts.
The enzyme live keeping agent is mixed by trehalose, glycerine and xanthans, the trehalose, glycerine, the matter of xanthans
Amount is than being (1-3)：(1-3)：(1-3).
The prebiotic bacterium solution is made up of the raw material of following mass percents：Water 85-95%, soy meal 4-14% and strain
0.5-1.5%.
The strain is mixed by aspergillus oryzae, candida utili and Lactobacillus plantarum, the aspergillus oryzae, Candida utilis
The mass ratio of yeast and Lactobacillus plantarum is (1-2)：(1-2)：(1-2).
Present invention also offers a kind of preparation method of the composite plant ferment containing probiotics, comprise the following steps：
(1) plant material is pre-processed：Plant material is crushed；
(2) enzyme live keeping agent is added：Enzyme live keeping agent is added into the plant material after crushing；
(3) ferment：Prebiotic bacterium solution is added, is well mixed, sealing, anaerobic fermentation；
(4) filter.
Preferably,
The temperature of step (3) anaerobic fermentation is 20 DEG C -50 DEG C, and the time is 15-1500 days, preferably 800-1200 days.
Step (4) filtering is filtered 2-3 times using plate and frame filter, and using the filter cloth of 180 mesh, control pressure is 0.2-
0.4Mpa, obtains filtrate, is the composite plant ferment containing probiotics.
Aspergillus oryzae, candida utili and Lactobacillus plantarum and soy meal are added to the water, you can the step (3) is made
In prebiotic bacterium solution.
Specifically, in the present invention：
Trehalose, No. CAS：99-20-7.
Glycerine, No. CAS：56-81-5.
Xanthans, No. CAS：11138-66-2.
Rice sprout, Chinese medicine name.Grass rice (scientific name：Oryza sativa L.) ripening fruits through germination dry and
Into.
Mulberry leaf, Chinese medicine name.Scientific name：Folium Mori.The dried leaf of moraceae plants mulberry.
Hawthorn, scientific name：Crataegus pinnatifida.Hawthorn also cries large-fruited Chinese hawthorn, haw, kermes fruit, is that the rose family is planted
The dry mature fruit of thing hawthorn.
Duck wheat, scientific name：Fagopyrum tataricum(L.)Gaertn.Alias spinach wheat, black wheat, flower buckwheat etc., it is annual
Herbaceous plant.
Balloonflower root, scientific name：Platycodon grandiflorus.Alias burden flower, small bell flower, mitral flower, are perennial grasses
This plant, stem is high 20~120 centimetres, generally hairless, even close by undercoat, not branch, few top branch.
Reed rhizome, Chinese medicine name is the root of reed.
Buddha's hand, scientific name：Citrus medica L.var.sarcodactylis Swingle.Fruit each heart in maturation
Skin is separated, and forms the fruit lobe of elongate curved, shape such as finger, therefore named Buddha's hand.
Almond, scientific name：Amygdalus Communis Vas.It is the seed of rose family apricot for dessert almond.
Radix glycyrrhizae, scientific name：Glycyrrhiza uralensis Fisch.Alias：State is old, Herba Hedyotis cantonensis, Glycyrrhiza Uralensis, sweet tea root
Son.
Dried orange peel, Chinese medicine name.For rutaceae orange (scientific name：Citrus reticulata Blanco) drying and ripening fruit
Skin.
Poria cocos, Chinese medicine name.For the dry sclerotia of On Polyporaceae Poria cocos.
Lily, scientific name：Lilium brownii var.viridulum Baker.Also known as strong another name for Sichuan Province, kind fragrant-flowered garlic, morningstar lily, fall it is celestial,
Again step, Zhongting, sieve that rubs, loaded van, in meet flower, lily garlic, cook's garlic, garlic brain potato, silk tree etc..
Purple perilla, scientific name：Perilla frutescens(L.)Britt.Alias：Osmanthus is weak, common perilla, red Soviet Union etc.；For Labiatae
Annual herb plant.
Aspergillus oryzae, scientific name：Aspergillus oryzae.Aspergillus oryzae belongs to Deuteromycotina, Hyphomycetes, hyphomycetales, from stalk
A Common Species in spore section, aspergillus fungi.
Candida utili, scientific name：Candida utilis.Candida utili is called Torula utilis Henneb or edible circle ferment
It is female.The content of its protein and vitamin B is all higher than brewer's yeast, and it can be using urea and nitric acid as nitrogen source, in the medium
Any growth factor, which need not be added, to be grown.
Lactobacillus plantarum, scientific name：lactobacillus plantarum.Lactobacillus plantarum is one kind of lactic acid bacteria, most suitable
Growth temperature is 30-35 DEG C, anaerobism or amphimicrobian, strain be it is straight or curved shaft-like, it is single, in pairs or into chain, belong to same
Type fermentative lactobacillus.
Composite plant ferment and its preparation of a kind of composite plant ferment containing probiotics that the present invention is provided containing probiotics
Method, preparation condition simulates the natural formation condition of ferment, and scientific in principle, equipment is simply suitable to large-scale production, the master of ferment
Composition is wanted in addition to multiple eating ferment, also has trace element, mineral matter, vitamin, amino acid, protein, carbohydrate etc.；
After enzyme live keeping agent, the activity of ferment is protected, and various extracellular ferment are complete, energetic, and these
Beneficial viable bacteria can largely pass through stomach, be colonized in enteron aisle, amount reproduction, produce various ferment, effectively decompose intestines
Food and removing enteron aisle rubbish in road, maintain the beneficial microbe colony of enteron aisle, the absorption beneficial to enteron aisle to various nutrition.
Embodiment
With reference to embodiment, the present invention is described further, as described below, is only the preferable implementation to the present invention
Example, not does the limitation of other forms, any those skilled in the art are possibly also with the disclosure above to the present invention
Technology contents be changed to the equivalent embodiment changed on an equal basis.It is every without departing from the present invention program content, according to the present invention
Technical spirit any simple modification that following examples are made or equivalent variations, all fall within protection scope of the present invention.
Embodiment 1
Composite plant ferment containing probiotics, is prepared from by the raw material of following weight parts：40 parts of rice sprout, 40 parts of mulberry leaf,
40 parts of hawthorn, 40 parts of duck wheat, 40 parts of balloonflower root, 40 parts of reed rhizome, 40 parts of Buddha's hand, 40 parts of almond, 40 parts of radix glycyrrhizae, 40 parts of dried orange peel, Fu
20 parts of Siberian cocklebur, 40 parts of lily, 40 parts of purple perilla, 15 parts of enzyme live keeping agent, prebiotic 190 parts of bacterium solution.
The enzyme live keeping agent is mixed by trehalose, glycerine and xanthans, the trehalose, glycerine, the matter of xanthans
Amount is than being 1：1：1.
The prebiotic bacterium solution is made up of the raw material of following mass percents：Water 90%, soy meal 9% and strain 1%.
The strain is mixed by aspergillus oryzae, candida utili and Lactobacillus plantarum, the aspergillus oryzae, Candida utilis
The mass ratio of yeast and Lactobacillus plantarum is 1：1：1.Strain and soy meal are added to the water, are uniformly mixed, you can is made
The prebiotic bacterium solution.
The specific preparation method of the above-mentioned composite plant ferment containing probiotics, comprises the following steps：
(1) plant material is pre-processed：Each plant material is weighed by proportioning, plant material cleaning is crushed；
(2) enzyme live keeping agent is added：Enzyme live keeping agent is added into the plant material after crushing；
(3) ferment：Prebiotic bacterium solution is added in plant material after being handled to step (2), is well mixed, sealing, anaerobism hair
Ferment, the temperature of fermentation is 35 DEG C, and the time is 1000 days；
(4) filter, filtering is using plate and frame filter filter 23, and using the filter cloth of 180 mesh, control pressure is 0.3Mpa,
Filtrate is obtained, is the composite plant ferment containing probiotics of the present invention.
Embodiment 2
Prepare, differ only according to the raw material proportioning described in embodiment 1 and method：The enzyme live keeping agent is by marine alga
Sugar, glycerine are mixed, and the trehalose, the mass ratio of glycerine are 1：1.Obtain the composite plant containing probiotics of embodiment 2
Ferment.
Embodiment 3
Prepare, differ only according to the raw material proportioning described in embodiment 1 and method：The enzyme live keeping agent is by marine alga
Sugar, xanthans are mixed, and the trehalose, the mass ratio of xanthans are 1：1.Obtain the compound containing probiotics of embodiment 3
Plant enzyme.
Embodiment 4
Prepare, differ only according to the raw material proportioning described in embodiment 1 and method：The enzyme live keeping agent by glycerine,
Xanthans is mixed, and the glycerine, the mass ratio of xanthans are 1：1.Obtain the composite plant ferment containing probiotics of embodiment 4
Element.
Comparative example 1
Prepare, but operated without step (2) according to the raw material proportioning described in embodiment 1 and method, i.e., not to crushing after
Plant material carries out enzyme-added live keeping agent processing.
Test case 1
Using prolease activity in determined by ultraviolet spectrophotometry ferment, determined using assay NBT photoreduction in ferment
SOD vigor, reference《National clinical examination operational procedure》Amylase activity is determined using iodine colorimetry, with reference to QB/T1803-
1993, using titration measuring lipase activity.The composite plant ferment containing probiotics prepared to embodiment 1-4 and comparative example 1
Enzyme activity is measured in element, and test result is shown in Table 1：
The primary efficacy enzyme activity unit of table 1：U/mL
| Enzyme class | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 1 |
| Protease | 2578.7 | 2314.1 | 2201.3 | 2039.7 | 1612.2 |
| Amylase | 40.6 | 33.1 | 32.8 | 31.5 | 26.1 |
| Lipase | 3.5 | 2.6 | 2.4 | 2.4 | 1.7 |
| Superoxide dismutase | 371.2 | 335.1 | 332.0 | 322.9 | 309.7 |
Claims (4)
1. a kind of composite plant ferment containing probiotics, it is characterised in that be prepared from by the raw material of following weight parts：Rice sprout
30-50 parts, 30-50 parts of mulberry leaf, 30-50 parts of hawthorn, 30-50 parts of duck wheat, 30-50 parts of balloonflower root, 30-50 parts of reed rhizome, Buddha's hand 30-
50 parts, 30-50 parts of almond, 30-50 parts of radix glycyrrhizae, 30-50 parts of dried orange peel, 10-30 parts of Poria cocos, 30-50 parts of lily, 30-50 parts of purple perilla,
10-20 parts of enzyme live keeping agent, prebiotic bacterium solution 150-200 parts；
The enzyme live keeping agent is mixed by trehalose, glycerine and xanthans, the trehalose, glycerine, the mass ratio of xanthans
For (1-3)：(1-3)：(1-3)；
The prebiotic bacterium solution is made up of the raw material of following mass percents：Water 85-95%, soy meal 4-14% and strain 0.5-
1.5%；The strain is mixed by aspergillus oryzae, candida utili and Lactobacillus plantarum, the aspergillus oryzae, Candida utilis ferment
The mass ratio of female and Lactobacillus plantarum is (1-2)：(1-2)：(1-2).
2. the preparation method of the composite plant ferment as claimed in claim 1 containing probiotics, it is characterised in that including following step
Suddenly：
(1) plant material is pre-processed：Plant material is crushed；
(2) enzyme live keeping agent is added：Enzyme live keeping agent is added into the plant material after crushing；
(3) ferment：Prebiotic bacterium solution is added, is well mixed, sealing, anaerobic fermentation；
(4) filter.
3. the preparation method of the composite plant ferment as claimed in claim 2 containing probiotics, it is characterised in that the step
(3) temperature of anaerobic fermentation is 20 DEG C -50 DEG C in, and the time is 15-1500 days.
4. the preparation method of the composite plant ferment as claimed in claim 2 containing probiotics, it is characterised in that the step
(4) filtering is filtered 2-3 times using plate and frame filter, and using the filter cloth of 180 mesh, control pressure is 0.2-0.4Mpa.
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| CN105918955A (en) * | 2016-05-10 | 2016-09-07 | 江西蓓蕾食品有限公司 | Lily and kudzu root compound enzyme and preparation method thereof |
| CN106136240A (en) * | 2016-07-12 | 2016-11-23 | 江门光大生物科技有限公司 | A kind of production method of Xinhui citrus reticulata acid discharge ferment |
| CN107594511A (en) * | 2017-10-30 | 2018-01-19 | 北京农学院 | A kind of buckwheat ferment and preparation method thereof |
| CN110074402A (en) * | 2019-05-10 | 2019-08-02 | 新昌县大船畈生物科技有限公司 | Plant enzyme and preparation method thereof |
| CN113558232A (en) * | 2021-07-16 | 2021-10-29 | 广西五福生食品有限公司 | Preparation method of live-bacteria tea enzyme capable of promoting growth of intestinal probiotics |
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| CN103315346B (en) * | 2013-06-17 | 2014-07-16 | 湖北富程祥云生物科技有限公司 | Preparation method of plant-enzyme-containing drink capable of improving respiratory system |
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