CN105211874A - Containing the composite plant ferment and preparation method thereof of probio - Google Patents
Containing the composite plant ferment and preparation method thereof of probio Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of composite plant ferment containing probio and preparation method thereof; the described composite plant ferment containing probio is prepared from by the raw material of following weight parts: rice sprout 30-50 part, mulberry leaf 30-50 part, hawthorn 30-50 part, duck wheat 30-50 part, balloonflower root 30-50 part, reed rhizome 30-50 part, Buddha's hand 30-50 part, almond 30-50 part, Radix Glycyrrhizae 30-50 part, dried orange peel 30-50 part, Poria cocos 10-30 part, lily 30-50 part, purple perilla 30-50 part; enzyme live keeping agent 10-20 part, probio liquid 150-200 part.After present invention uses enzyme live keeping agent; the activity of ferment is protected; the outer ferment of various born of the same parents is complete, energetic, and useful viable bacteria major part can pass through stomach smoothly; in enteron aisle under field planting; amount reproduction, produces various ferment, effectively decomposes the food in enteron aisle and removes enteron aisle rubbish; maintain the beneficial microbe colony of enteron aisle, be beneficial to the absorption of enteron aisle to various nutrition.
Description
Technical field
The present invention relates to nutraceutical field, be specifically related to a kind of composite plant ferment containing probio and preparation method thereof.
Background technology
Ferment is also called " enzyme " is the catalyst of various biochemical reaction, ferment is present in all living cells, is that it have activated the vigor of cell, makes cell show all signs of life, the prosperous Bert Sheng Tinuo of U.S. naturopathy doctor says: human body as bulb, ferment image current.Having the bulb after energising only just can be bright, without electricity, we have just one can not be bright bulb.Therefore, ferment is the indispensable important substance of human body.Ferment is divided into two large classes, external ferment and body endo enzyme, and the ferment in human body is limited, when the effect of body endo enzyme is weak or reduce, disease will be following, and therefore human body reasonably should meet metabolic needs from external supplementary ferment, the invasion of resist the disease.
Ferment is so important, closely bound up with life, but people's rhythm of life is accelerated now, and residing Environment pollution is serious, and most of people are under the state of inferior health, adds that the enzyme of disappearance in human body of not focusing on causing of diet gets more and more.We must absorb more ferment from meals, and food is after more than 54 DEG C high temperature or fine finishining, makes again the ferment in food be destroyed.In order to the ferment of supplementary needed by human body, ferment technology is developed, ferment technology refers to the technology that multi-cultur es compound microbial colony ferments, and be a novel new and high technology, traditional product such as pickles, thick broad-bean sauce, natto, miso etc. also can be called ferment product.Refer in particular to the plant such as fresh vegetables, fruit for raw material herein, produce through multiple probiotics fermention, the microorganism formulation containing abundant enzyme, vitamin, mineral matter and secondary metabolite.Common fruit, vegetables, brown rice, mushroom class, integration of drinking and medicinal herbs Chinese medicine etc. can as the raw materials of ferment fermentation.Protease, lipase and superoxide dismutase etc. are the primary efficacy enzymes of enzyme microb class health food.
For making enzyme food more meet modern to diversity, the balance of nutritional need be easy to absorbability, no matter be that traditional handicraft or modern crafts still need further improvement.Although domestic market has also occurred some add the health food of ferment, in the application of other field of food also seldom, still there is wide development space.Given this, enzyme food is the necessity that production technology or the application in food all make further progress.Therefore, manufacture craft and the product of developing a kind of composite plant ferment containing probio are new problems anxious to be resolved always.
Summary of the invention
For above shortcomings in prior art, technical problem to be solved by this invention is to provide a kind of composite plant ferment containing probio and preparation method thereof.
The present invention seeks to be achieved through the following technical solutions:
Containing a composite plant ferment for probio, be prepared from by the raw material of following weight parts:
Rice sprout 30-50 part, mulberry leaf 30-50 part, hawthorn 30-50 part, duck wheat 30-50 part, balloonflower root 30-50 part, reed rhizome 30-50 part, Buddha's hand 30-50 part, almond 30-50 part, Radix Glycyrrhizae 30-50 part, dried orange peel 30-50 part, Poria cocos 10-30 part, lily 30-50 part, purple perilla 30-50 part; enzyme live keeping agent 10-20 part, probio liquid 150-200 part.
Described enzyme live keeping agent is mixed by trehalose, glycerine and xanthans, and the mass ratio of described trehalose, glycerine, xanthans is (1-3): (1-3): (1-3).
Described probio liquid is made up of the raw material of following mass percent: water 85-95%, soy meal 4-14% and bacterial classification 0.5-1.5%.
Described bacterial classification is mixed by aspergillus oryzae, candida utili and Lactobacillus plantarum, and the mass ratio of described aspergillus oryzae, candida utili and Lactobacillus plantarum is (1-2): (1-2): (1-2).
Present invention also offers the preparation method of described a kind of composite plant ferment containing probio, comprise the following steps:
(1) plant material pretreatment: plant material is pulverized;
(2) enzyme live keeping agent is added: in the plant material after pulverizing, add enzyme live keeping agent;
(3) ferment: add probio liquid again, mix, sealing, anaerobic fermentation;
(4) filter.
Preferably,
The temperature of step (3) anaerobic fermentation is 20 DEG C-50 DEG C, and the time is 15-1500 days, is preferably 800-1200 days.
Step (4) is filtered and is used plate and frame filter to filter 2-3 time, and adopt 180 object filter clothes, controlled pressure is 0.2-0.4Mpa, obtains filtrate, is the composite plant ferment containing probio.
Aspergillus oryzae, candida utili and Lactobacillus plantarum and soy meal are added to the water, the probio liquid in described step (3) can be obtained.
Concrete, in the present invention:
Trehalose, No. CAS: 99-20-7.
Glycerine, No. CAS: 56-81-5.
Xanthans, No. CAS: 11138-66-2.
Rice sprout, Chinese medicine name.The ripening fruits of grass rice (formal name used at school: OryzasativaL.) forms through drying of germinateing.
Mulberry leaf, Chinese medicine name.Formal name used at school: FoliumMori.The dry leaf of moraceae plants mulberry.
Hawthorn, formal name used at school: Crataeguspinnatifida.Hawthorn also cries large-fruited Chinese hawthorn, haw, kermes fruit, is the dry mature fruit of rosaceous plant hawthorn.
Duck wheat, formal name used at school: Fagopyrumtataricum (L.) Gaertn.Another name spinach wheat, Wu Mai, Hua Qiao etc., annual herb plant.
Balloonflower root, formal name used at school: Platycodongrandiflorus.Another name burden flower, small bell flower, mitral flower, be herbaceos perennial, stem is high 20 ~ 120 centimetres, usually without hair, even close by undercoat, not branch, few top branch.
Reed rhizome, Chinese medicine name is the root of reed.
Buddha's hand, formal name used at school: CitrusmedicaL.var.sarcodactylisSwingle.Fruit each carpel when maturation is separated, and forms the fruit lobe of elongate curved, shape as finger, therefore named Buddha's hand.
Almond, formal name used at school: AmygdalusCommunisVas.For dessert almond, it is the seed of rose family apricot.
Radix Glycyrrhizae, formal name used at school: GlycyrrhizauralensisFisch.Another name: old, the sweet grass of state, Glycyrrhiza Uralensis, sweet root.
Dried orange peel, Chinese medicine name.For the dry mature skin of rutaceae orange (formal name used at school: CitrusreticulataBlanco).
Poria cocos, Chinese medicine name.For the dry sclerotia of On Polyporaceae Poria cocos.
Lily, formal name used at school: Liliumbrowniivar.viridulumBaker.Have another name called strong another name for Sichuan Province, kind fragrant-flowered garlic, morningstar lily, fall celestial, heavily step, Zhongting, sieve that rubs, loaded van, in meet flower, lily garlic, cook's garlic, garlic brain potato, silk tree etc.
Purple perilla, formal name used at school: Perillafrutescens (L.) Britt.Another name: osmanthus is weak, common perilla, red Soviet Union etc.; For Labiatae annual herb plant.
Aspergillus oryzae, formal name used at school: Aspergillusoryzae.Aspergillus oryzae belongs to Deuteromycotina, Hyphomycetes, hyphomycetales, from Geng Bao section, and a Common Species in aspergillus fungi.
Candida utili, formal name used at school: Candidautilis.Candida utili is again Torula utilis Henneb or edible torula.The content of its protein and Cobastab is all high than brewer's yeast, and it can using urea and nitric acid as nitrogenous source, and not needing to add any growth factor in the medium can grow.
Lactobacillus plantarum, formal name used at school: lactobacillusplantarum.Lactobacillus plantarum is the one of lactic acid bacteria, and optimum growth temperature is 30-35 DEG C, anaerobism or amphimicrobian, and bacterial classification is straight or curved shaft-like, single, paired or one-tenth chain, belongs to homofermentative lactic bacteria.
A kind of composite plant ferment containing probio provided by the invention contains composite plant ferment of probio and preparation method thereof, the Nature creating condition of preparation condition simulation ferment, scientific in principle, equipment is simply suitable for large-scale production, the main component of ferment, except multiple eating ferment, also has trace element, mineral matter, vitamin, amino acid, protein, carbohydrate etc.; Especially after employing enzyme live keeping agent; the activity of ferment is protected; the outer ferment of various born of the same parents is complete, energetic, and these useful viable bacteria major parts can pass through stomach smoothly; in enteron aisle under field planting; amount reproduction, produces various ferment, effectively decomposes the food in enteron aisle and removes enteron aisle rubbish; maintain the beneficial microbe colony of enteron aisle, be beneficial to the absorption of enteron aisle to various nutrition.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed to the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, any simple modification done following examples according to technical spirit of the present invention or equivalent variations, all drop in protection scope of the present invention.
Embodiment 1
Containing the composite plant ferment of probio; be prepared from by the raw material of following weight parts: rice sprout 40 parts, 40 parts, mulberry leaf, hawthorn 40 parts, duck wheat 40 parts, balloonflower root 40 parts, reed rhizome 40 parts, Buddha's hand 40 parts, 40 parts, almond, 40 parts, Radix Glycyrrhizae, dried orange peel 40 parts, 20 parts, Poria cocos, lily 40 parts, purple perilla 40 parts; enzyme live keeping agent 15 parts, probio liquid 190 parts.
Described enzyme live keeping agent is mixed by trehalose, glycerine and xanthans, and the mass ratio of described trehalose, glycerine, xanthans is 1:1:1.
Described probio liquid is made up of the raw material of following mass percent: water 90%, soy meal 9% and bacterial classification 1%.
Described bacterial classification is mixed by aspergillus oryzae, candida utili and Lactobacillus plantarum, and the mass ratio of described aspergillus oryzae, candida utili and Lactobacillus plantarum is 1:1:1.Bacterial classification and soy meal are added to the water, are uniformly mixed, described probio liquid can be obtained.
The concrete preparation method of the above-mentioned composite plant ferment containing probio, comprises the following steps:
(1) plant material pretreatment: take each plant material by proportioning, by plant material cleaning, pulverizes;
(2) enzyme live keeping agent is added: in the plant material after pulverizing, add enzyme live keeping agent;
(3) ferment: in the plant material after step (2) process, add probio liquid, mix, sealing, anaerobic fermentation, the temperature of fermentation is 35 DEG C, and the time is 1000 days;
(4) filter, filter and use plate and frame filter filter 23, adopt 180 object filter clothes, controlled pressure is 0.3Mpa, obtains filtrate, is the composite plant ferment containing probio of the present invention.
Embodiment 2
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described enzyme live keeping agent is mixed by trehalose, glycerine, and the mass ratio of described trehalose, glycerine is 1:1.Obtain the composite plant ferment containing probio of embodiment 2.
Embodiment 3
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described enzyme live keeping agent is mixed by trehalose, xanthans, and the mass ratio of described trehalose, xanthans is 1:1.Obtain the composite plant ferment containing probio of embodiment 3.
Embodiment 4
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described enzyme live keeping agent is mixed by glycerine, xanthans, and the mass ratio of described glycerine, xanthans is 1:1.Obtain the composite plant ferment containing probio of embodiment 4.
Comparative example 1
According to the pulp furnish described in embodiment 1 and method preparation, but do not carry out step (2) operation, namely enzyme-added live keeping agent process is not carried out to plant material after pulverizing.
Test case 1
Adopt prolease activity in determined by ultraviolet spectrophotometry ferment, assay NBT photoreduction is adopted to measure the vigor of SOD in ferment, adopt iodine colorimetry to measure amylase activity with reference to " national clinical examination operational procedure ", with reference to QB/T1803-1993, adopt titration measuring lipase activity.That prepares embodiment 1-4 and comparative example 1 measures containing enzyme activity in the composite plant ferment of probio, and test result is in table 1:
Table 1 primary efficacy enzyme activity unit: U/mL
| Enzyme class | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 1 |
| Protease | 2578.7 | 2314.1 | 2201.3 | 2039.7 | 1612.2 |
| Amylase | 40.6 | 33.1 | 32.8 | 31.5 | 26.1 |
| Lipase | 3.5 | 2.6 | 2.4 | 2.4 | 1.7 |
| Superoxide dismutase | 371.2 | 335.1 | 332.0 | 322.9 | 309.7 |
Claims (7)
1. the composite plant ferment containing probio; it is characterized in that; be prepared from by the raw material of following weight parts: rice sprout 30-50 part, mulberry leaf 30-50 part, hawthorn 30-50 part, duck wheat 30-50 part, balloonflower root 30-50 part, reed rhizome 30-50 part, Buddha's hand 30-50 part, almond 30-50 part, Radix Glycyrrhizae 30-50 part, dried orange peel 30-50 part, Poria cocos 10-30 part, lily 30-50 part, purple perilla 30-50 part; enzyme live keeping agent 10-20 part, probio liquid 150-200 part.
2. the composite plant ferment containing probio as claimed in claim 1; it is characterized in that; described enzyme live keeping agent is mixed by trehalose, glycerine and xanthans, and the mass ratio of described trehalose, glycerine, xanthans is (1-3): (1-3): (1-3).
3. the composite plant ferment containing probio as claimed in claim 1, it is characterized in that, described probio liquid is made up of the raw material of following mass percent: water 85-95%, soy meal 4-14% and bacterial classification 0.5-1.5%.
4. the composite plant ferment containing probio as claimed in claim 3, it is characterized in that, described bacterial classification is mixed by aspergillus oryzae, candida utili and Lactobacillus plantarum, and the mass ratio of described aspergillus oryzae, candida utili and Lactobacillus plantarum is (1-2): (1-2): (1-2).
5. the preparation method of the composite plant ferment containing probio as described in any one of claim 1-4, is characterized in that, comprise the following steps:
(1) plant material pretreatment: plant material is pulverized;
(2) enzyme live keeping agent is added: in the plant material after pulverizing, add enzyme live keeping agent;
(3) ferment: add probio liquid again, mix, sealing, anaerobic fermentation;
(4) filter.
6. the preparation method of the composite plant ferment containing probio as claimed in claim 5, is characterized in that, in described step (3), the temperature of anaerobic fermentation is 20 DEG C-50 DEG C, and the time is 15-1500 days.
7. the preparation method of the composite plant ferment containing probio as claimed in claim 5, is characterized in that, described step (4) is filtered and used plate and frame filter to filter 2-3 time, and adopt 180 object filter clothes, controlled pressure is 0.2-0.4Mpa.
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