CN105200008A - Stem cell culture medium - Google Patents
Stem cell culture medium Download PDFInfo
- Publication number
- CN105200008A CN105200008A CN201510717978.4A CN201510717978A CN105200008A CN 105200008 A CN105200008 A CN 105200008A CN 201510717978 A CN201510717978 A CN 201510717978A CN 105200008 A CN105200008 A CN 105200008A
- Authority
- CN
- China
- Prior art keywords
- growth factor
- recombinant human
- stem cell
- culture medium
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicinal Preparation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a stem cell culture medium. The stem cell culture medium comprises an improved DMEM (Dulbecco's Modified Eagle Medium)/F12 basal culture medium and an additive, wherein the additive comprises sodium bicarbonate, selenium amino acid chelate, recombinant human insulin growth factors, recombinant human basic fibroblast growth factors, recombinant human lactoferrin, ascorbic acid, recombinant human platelet-derived growth factors, recombinant human vascular endothelial cell growth factors, octacosanol, polyvinyl alcohol, polyvinylpyrrolidone and recombinant human epidermal growth factors. According to the stem cell culture medium, the potential of stem cells is not influenced while the stem cells can be proliferated rapidly, the proliferation speed of the stem cells is increased by 3-5 times compared with a common culture medium, and further, the stem cell culture medium can be used for culturing the stem cells of various kinds of tissue and has excellent applicability; the cultured stem cells have high differentiation capability, can be differentiated into multiple functional cells and have very high scientific research and medical application values, culture medium components are exact, the quality is stable, and accordingly, the cultured stem cells are not likely to generate human body rejection reaction after transplanting.
Description
Technical field
The present invention relates to and technical field of stem cell culture, be specifically related to a kind of stem cell media.
Background technology
Many research shows that stem cell not only can self, can be divided into other functioning cells when condition is suitable, and therefore stem cell is expected to the effective means becoming treatment mankind difficult diseases.But stem cell content in normal adult tissue is very micro-, in vitro how rapid amplifying and culturing stem cells cultivate be study stem cell mechanism of action and explore it at the important technology for the treatment of human disease treatment method.
Stem cell content of trying one's best is very micro-, but is distributed widely in each histoorgan mammiferous, and these tissues or organ are including, but not limited to marrow, umbilical cord, fatty tissue, cerebral tissue, retina, heart, liver, lung and skin etc.Large quantity research shows, the stem cell of these low levelss can be increased by appropriate means in vitro further, and the most conventional a kind of stem cell media is exactly the substratum being added with 10% foetal calf serum.But adopt the stem cell of this culture medium culturing, not only growth fraction is comparatively slow, and degree of transitivity reduces greatly more than the potential that after 5 generations, it is divided into functioning cell.
Summary of the invention
For solving the problem, the invention provides a kind of stem cell media.
For achieving the above object, the technical scheme that the present invention takes is:
Stem cell media, comprise improvement DMEM/F12 basic medium and additive, described additive comprises
Sodium bicarbonate 400-600 μ g/ml, selenium-amino acid chelate 8-20ng/ml, recombinant human insulin somatomedin 10-30ng/ml, recombination human basic fibroblast growth factor 80-120ng/ml, restructuring lactoferrin 7-13 μ g/ml, xitix 50-70 μ g/ml, derivative growth factor of recombined human blood platelet 3-7 μ g/ml, recombinant human vascular endothelial growth factor 1-7ng/ml, policosanol 0.1-10 μ g/ml, polyvinyl alcohol 0.1-10 μ g/ml, polyvinylpyrrolidone 0.1-10 μ g/ml, recombinant human epidermal growth factor 1-10 μ g/ml.
Preferably, described improvement DMEM/F12 basic medium comprises the cultivation of DMEM/F12 basis, VITAMIN and nutritive element.
Preferably, described VITAMIN comprises vitamins C 1-50 μ g/ml, vitamin B complex 30-100 μ g/ml, vitamin e1 0-100 μ g/mL.
Preferably, described nutritive element comprises L-glutaminate 1-5mg/ml, manganese methionine 1-10mg/ml, zinc chloride 1-5mg/ml, amino acid chelated magnesium 1-5mg/ml, Sethotope 1.5-5.5mg/ml, phytase 0.5-0.75mg/ml, pyridine acid chromium salt 0.055-0.065mg/ml, calcium iodate 0.1-0.3mg/ml, Methionin 1-6mg/ml, Hexammine cobaltic chloride 0.05-0.065mg/ml, albumen germanium 0.55-0.75mg/ml, iodine yeast 2-6mg/ml, cupric chloride 0.1-1mg/ml.
Preferably, described additive comprises sodium bicarbonate 400 μ g/ml, selenium-amino acid chelate 8ng/ml, recombinant human insulin somatomedin 10ng/ml, recombination human basic fibroblast growth factor 80ng/ml, restructuring lactoferrin 7 μ g/ml, xitix 50 μ g/ml, derivative growth factor of recombined human blood platelet 3 μ g/ml, recombinant human vascular endothelial growth factor 1ng/ml, policosanol 0.1 μ g/ml, polyvinyl alcohol 0.1 μ g/ml, polyvinylpyrrolidone 0.1 μ g/ml, recombinant human epidermal growth factor 1 μ g/ml.
Preferably, described additive comprises sodium bicarbonate 500 μ g/ml, selenium-amino acid chelate 14ng/ml, recombinant human insulin's growth factor-2 0ng/ml, recombination human basic fibroblast growth factor 100ng/ml, restructuring lactoferrin 10 μ g/ml, xitix 60 μ g/ml, derivative growth factor of recombined human blood platelet 5 μ g/ml, recombinant human endothelial cell growth factor 4 ng/ml, policosanol 5.05 μ g/ml, polyvinyl alcohol 5.05 μ g/ml, polyvinylpyrrolidone 5.05 μ g/ml, recombinant human epidermal growth factor 5.5 μ g/ml.
Preferably, described additive comprises sodium bicarbonate 600 μ g/ml, selenium-amino acid chelate 20ng/ml, recombinant human insulin somatomedin 30ng/ml, recombination human basic fibroblast growth factor 120ng/ml, restructuring lactoferrin 13 μ g/ml, xitix 70 μ g/ml, derivative growth factor of recombined human blood platelet 7 μ g/ml, recombinant human vascular endothelial growth factor 7ng/ml, policosanol 10 μ g/ml, polyvinyl alcohol 10 μ g/ml, polyvinylpyrrolidone 10 μ g/ml, recombinant human epidermal growth factor-1 0 μ g/ml.
The present invention has following beneficial effect:
Base can not affect again the potential of stem cell by rapid amplifying stem cell simultaneously, amplification rate comparatively conventional medium raising 3-5 times of stem cell, and the stem cell cultivating Various Tissues can be used for, have splendid suitability, the differentiation of stem cells ability of cultivating is strong, can be divided into several functions cell, there is very high scientific research and medical use value, medium component is clear and definite, stay in grade, makes the stem cell cultivated be not easy after the transfer to produce human body rejection.
Embodiment
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiment 1
A kind of stem cell media, comprises DMEM/F12 basis and cultivates, vitamins C 1 μ g/ml, vitamin B complex 30 μ g/ml, vitamin e1 0 μ g/mL, L-glutaminate 1mg/ml, manganese methionine 1mg/ml, zinc chloride 1mg/ml, amino acid chelated magnesium 1mg/ml, Sethotope 1.5mg/ml, phytase 0.5mg/ml, pyridine acid chromium salt 0.055mg/ml, calcium iodate 0.1mg/ml, Methionin 1mg/ml, Hexammine cobaltic chloride 0.05mg/ml, albumen germanium 0.55mg/ml, iodine yeast 2mg/ml, cupric chloride 0.1mg/ml, sodium bicarbonate 400 μ g/ml, selenium-amino acid chelate 8ng/ml, recombinant human insulin somatomedin 10ng/ml, recombination human basic fibroblast growth factor 80ng/ml, restructuring lactoferrin 7 μ g/ml, xitix 50 μ g/ml, derivative growth factor of recombined human blood platelet 3 μ g/ml, recombinant human vascular endothelial growth factor 1ng/ml, policosanol 0.1 μ g/ml, polyvinyl alcohol 0.1 μ g/ml, polyvinylpyrrolidone 0.1 μ g/ml, recombinant human epidermal growth factor 1 μ g/ml.
Embodiment 2
A kind of stem cell media, comprises DMEM/F12 basis and cultivates, vitamins C 50 μ g/ml, vitamin B complex 100 μ g/ml, vitamin e1 00 μ g/mL, L-glutaminate 5mg/ml, manganese methionine 10mg/ml, zinc chloride 5mg/ml, amino acid chelated magnesium 5mg/ml, Sethotope 5.5mg/ml, phytase 0.75mg/ml, pyridine acid chromium salt 0.065mg/ml, calcium iodate 0.3mg/ml, Methionin 6mg/ml, Hexammine cobaltic chloride 0.065mg/ml, albumen germanium 0.75mg/ml, iodine yeast 6mg/ml, cupric chloride 1mg/ml, sodium bicarbonate 500 μ g/ml, selenium-amino acid chelate 14ng/ml, recombinant human insulin's growth factor-2 0ng/ml, recombination human basic fibroblast growth factor 100ng/ml, restructuring lactoferrin 10 μ g/ml, xitix 60 μ g/ml, derivative growth factor of recombined human blood platelet 5 μ g/ml, recombinant human endothelial cell growth factor 4 ng/ml, policosanol 5.05 μ g/ml, polyvinyl alcohol 5.05 μ g/ml, polyvinylpyrrolidone 5.05 μ g/ml, recombinant human epidermal growth factor 5.5 μ g/ml.
Embodiment 3
A kind of stem cell media, comprises DMEM/F12 basis and cultivates, Catergen 5.5 μ g/ml, vitamin B complex 65 μ g/ml, vitamin-E 55 μ g/mL, L-glutaminate 3mg/ml, manganese methionine 5.5mg/ml, zinc chloride 3mg/ml, amino acid chelated magnesium 3mg/ml, Sethotope 3.5mg/ml, phytase 0.625mg/ml, pyridine acid chromium salt 0.06mg/ml, calcium iodate 0.2mg/ml, Methionin 3.5mg/ml, Hexammine cobaltic chloride 0.0575mg/ml, albumen germanium 0.65mg/ml, iodine yeast 4mg/ml, cupric chloride 0.55mg/ml, sodium bicarbonate 600 μ g/ml, selenium-amino acid chelate 20ng/ml, recombinant human insulin somatomedin 30ng/ml, recombination human basic fibroblast growth factor 120ng/ml, restructuring lactoferrin 13 μ g/ml, xitix 70 μ g/ml, derivative growth factor of recombined human blood platelet 7 μ g/ml, recombinant human vascular endothelial growth factor 7ng/ml, policosanol 10 μ g/ml, polyvinyl alcohol 10 μ g/ml, polyvinylpyrrolidone 10 μ g/ml, recombinant human epidermal growth factor-1 0 μ g/ml.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. stem cell media, is characterized in that, comprise improvement DMEM/F12 basic medium and additive, described additive comprises
Sodium bicarbonate 400-600 μ g/ml, selenium-amino acid chelate 8-20ng/ml, recombinant human insulin somatomedin 10-30ng/ml, recombination human basic fibroblast growth factor 80-120ng/ml, restructuring lactoferrin 7-13 μ g/ml, xitix 50-70 μ g/ml, derivative growth factor of recombined human blood platelet 3-7 μ g/ml, recombinant human vascular endothelial growth factor 1-7ng/ml, policosanol 0.1-10 μ g/ml, polyvinyl alcohol 0.1-10 μ g/ml, polyvinylpyrrolidone 0.1-10 μ g/ml, recombinant human epidermal growth factor 1-10 μ g/ml.
2. stem cell media according to claim 1, is characterized in that, described improvement DMEM/F12 basic medium comprises the cultivation of DMEM/F12 basis, VITAMIN and nutritive element.
3. stem cell media according to claim 2, is characterized in that, described VITAMIN comprises vitamins C 1-50 μ g/ml, vitamin B complex 30-100 μ g/ml, vitamin e1 0-100 μ g/mL.
4. stem cell media according to claim 2, it is characterized in that, described nutritive element comprises L-glutaminate 1-5mg/ml, manganese methionine 1-10mg/ml, zinc chloride 1-5mg/ml, amino acid chelated magnesium 1-5mg/ml, Sethotope 1.5-5.5mg/ml, phytase 0.5-0.75mg/ml, pyridine acid chromium salt 0.055-0.065mg/ml, calcium iodate 0.1-0.3mg/ml, Methionin 1-6mg/ml, Hexammine cobaltic chloride 0.05-0.065mg/ml, albumen germanium 0.55-0.75mg/ml, iodine yeast 2-6mg/ml, cupric chloride 0.1-1mg/ml.
5. stem cell media according to claim 1, it is characterized in that, described additive comprises sodium bicarbonate 400 μ g/ml, selenium-amino acid chelate 8ng/ml, recombinant human insulin somatomedin 10ng/ml, recombination human basic fibroblast growth factor 80ng/ml, restructuring lactoferrin 7 μ g/ml, xitix 50 μ g/ml, derivative growth factor of recombined human blood platelet 3 μ g/ml, recombinant human vascular endothelial growth factor 1ng/ml, policosanol 0.1 μ g/ml, polyvinyl alcohol 0.1 μ g/ml, polyvinylpyrrolidone 0.1 μ g/ml, recombinant human epidermal growth factor 1 μ g/ml.
6. stem cell media according to claim 1, it is characterized in that, described additive comprises sodium bicarbonate 500 μ g/ml, selenium-amino acid chelate 14ng/ml, recombinant human insulin's growth factor-2 0ng/ml, recombination human basic fibroblast growth factor 100ng/ml, restructuring lactoferrin 10 μ g/ml, xitix 60 μ g/ml, derivative growth factor of recombined human blood platelet 5 μ g/ml, recombinant human endothelial cell growth factor 4 ng/ml, policosanol 5.05 μ g/ml, polyvinyl alcohol 5.05 μ g/ml, polyvinylpyrrolidone 5.05 μ g/ml, recombinant human epidermal growth factor 5.5 μ g/ml.
7. stem cell media according to claim 1, it is characterized in that, described additive comprises sodium bicarbonate 600 μ g/ml, selenium-amino acid chelate 20ng/ml, recombinant human insulin somatomedin 30ng/ml, recombination human basic fibroblast growth factor 120ng/ml, restructuring lactoferrin 13 μ g/ml, xitix 70 μ g/ml, derivative growth factor of recombined human blood platelet 7 μ g/ml, recombinant human vascular endothelial growth factor 7ng/ml, policosanol 10 μ g/ml, polyvinyl alcohol 10 μ g/ml, polyvinylpyrrolidone 10 μ g/ml, recombinant human epidermal growth factor-1 0 μ g/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510717978.4A CN105200008A (en) | 2015-10-23 | 2015-10-23 | Stem cell culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510717978.4A CN105200008A (en) | 2015-10-23 | 2015-10-23 | Stem cell culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105200008A true CN105200008A (en) | 2015-12-30 |
Family
ID=54947971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510717978.4A Pending CN105200008A (en) | 2015-10-23 | 2015-10-23 | Stem cell culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105200008A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148275A (en) * | 2016-08-08 | 2016-11-23 | 安徽惠恩生物科技股份有限公司 | A kind of culture medium improving the adherent property of epidermal stem cells |
| CN106282114A (en) * | 2016-08-08 | 2017-01-04 | 安徽惠恩生物科技股份有限公司 | A kind of fast breeding nerve stem cell culture medium |
| CN106834220A (en) * | 2016-12-24 | 2017-06-13 | 叶宗耀 | A kind of serum-free cultured chondrocytes base and preparation method thereof |
| CN107164325A (en) * | 2017-06-28 | 2017-09-15 | 北京再生生物科技研究院有限公司 | The preparation method and kit of the oligodendroglia in MSCs sources |
| CN107653222A (en) * | 2016-07-25 | 2018-02-02 | 赵玉明 | A kind of collocation method of sterile liquid cell culture medium |
| CN107686827A (en) * | 2017-10-30 | 2018-02-13 | 洛阳轩智生物科技有限公司 | Human adipose-derived stem cell special media |
| CN108699519A (en) * | 2016-01-12 | 2018-10-23 | 康干细胞生物技术有限公司 | The stem cell-derived allochthon containing the raised growth factor |
| CN111206017A (en) * | 2019-04-30 | 2020-05-29 | 浙江大学 | Serum-free culture medium for stem cells and application thereof |
| CN113166723A (en) * | 2018-12-07 | 2021-07-23 | 关东化学株式会社 | Undifferentiated maintenance medium for pluripotent stem cells |
| WO2021220731A1 (en) * | 2020-04-30 | 2021-11-04 | オリエンタル酵母工業株式会社 | Stem cell medium and stem cell culturing method |
| WO2021227573A1 (en) * | 2020-05-14 | 2021-11-18 | 梦芊科技知识产权有限公司 | Xeno-free culture medium and method for expansion of mesenchymal stem cells by means of using same |
| CN114134108A (en) * | 2021-11-30 | 2022-03-04 | 武汉伯韬生物科技有限公司 | Stem cell bioactive composition and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130071932A1 (en) * | 2010-03-23 | 2013-03-21 | Public University Corporation Yokohama City University | Culture method for causing differentiation of pluripotent mammalian cells |
| CN103243070A (en) * | 2013-04-25 | 2013-08-14 | 刘小青 | Stem cell medium and application thereof |
| CN103952374A (en) * | 2014-05-07 | 2014-07-30 | 北京赛贝生物技术有限公司 | Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells |
| CN104357379A (en) * | 2014-09-30 | 2015-02-18 | 刘兴宇 | Stem cell culture medium |
-
2015
- 2015-10-23 CN CN201510717978.4A patent/CN105200008A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130071932A1 (en) * | 2010-03-23 | 2013-03-21 | Public University Corporation Yokohama City University | Culture method for causing differentiation of pluripotent mammalian cells |
| CN103243070A (en) * | 2013-04-25 | 2013-08-14 | 刘小青 | Stem cell medium and application thereof |
| CN103952374A (en) * | 2014-05-07 | 2014-07-30 | 北京赛贝生物技术有限公司 | Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells |
| CN104357379A (en) * | 2014-09-30 | 2015-02-18 | 刘兴宇 | Stem cell culture medium |
Non-Patent Citations (2)
| Title |
|---|
| O. KILIAN ET.AL.: "EFFECTS OF PLATELET GROWTH FACTORS ON HUMAN MESENCHYMAL STEM CELLS AND HUMAN ENDOTHELIAL CELLS IN VITRO", 《EUROPEAN JOURNAL OF MEDICAL RESEARCH》 * |
| 李丽娜等: "人脐带间充质干细胞条件培养基体外支持造血的功能", 《中国实验血液学杂志》 * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108699519A (en) * | 2016-01-12 | 2018-10-23 | 康干细胞生物技术有限公司 | The stem cell-derived allochthon containing the raised growth factor |
| CN107653222A (en) * | 2016-07-25 | 2018-02-02 | 赵玉明 | A kind of collocation method of sterile liquid cell culture medium |
| CN106282114A (en) * | 2016-08-08 | 2017-01-04 | 安徽惠恩生物科技股份有限公司 | A kind of fast breeding nerve stem cell culture medium |
| CN106148275A (en) * | 2016-08-08 | 2016-11-23 | 安徽惠恩生物科技股份有限公司 | A kind of culture medium improving the adherent property of epidermal stem cells |
| CN106834220A (en) * | 2016-12-24 | 2017-06-13 | 叶宗耀 | A kind of serum-free cultured chondrocytes base and preparation method thereof |
| CN107164325A (en) * | 2017-06-28 | 2017-09-15 | 北京再生生物科技研究院有限公司 | The preparation method and kit of the oligodendroglia in MSCs sources |
| CN107686827A (en) * | 2017-10-30 | 2018-02-13 | 洛阳轩智生物科技有限公司 | Human adipose-derived stem cell special media |
| CN107686827B (en) * | 2017-10-30 | 2018-11-16 | 集钧（上海）医疗科技发展有限公司 | Human adipose-derived stem cell special media |
| CN113166723A (en) * | 2018-12-07 | 2021-07-23 | 关东化学株式会社 | Undifferentiated maintenance medium for pluripotent stem cells |
| CN113166723B (en) * | 2018-12-07 | 2024-08-30 | 关东化学株式会社 | Undifferentiated maintenance medium for pluripotent stem cells |
| CN111206017A (en) * | 2019-04-30 | 2020-05-29 | 浙江大学 | Serum-free culture medium for stem cells and application thereof |
| CN111206017B (en) * | 2019-04-30 | 2022-02-18 | 浙江大学 | Serum-free culture medium for stem cells and application thereof |
| WO2021220731A1 (en) * | 2020-04-30 | 2021-11-04 | オリエンタル酵母工業株式会社 | Stem cell medium and stem cell culturing method |
| WO2021227573A1 (en) * | 2020-05-14 | 2021-11-18 | 梦芊科技知识产权有限公司 | Xeno-free culture medium and method for expansion of mesenchymal stem cells by means of using same |
| CN114134108A (en) * | 2021-11-30 | 2022-03-04 | 武汉伯韬生物科技有限公司 | Stem cell bioactive composition and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105200008A (en) | Stem cell culture medium | |
| CN106386511B (en) | The rapid propagation method of beautiful millettia root suspension cell culture | |
| CN104818248A (en) | Immunocyte culture medium, and culture method and application of immunocytes | |
| CN103911339A (en) | Serum-free fibroblast cell culture medium and preparation method thereof | |
| CN103892140B (en) | A kind of feed addictive improving egg-shaped pompano sugar utilization | |
| CN106520679B (en) | It is a kind of for cultivating the kit of pancreatic stem cells | |
| CN103766218A (en) | Butterfly orchid induction culture medium and asexual propagation method of butterfly orchid | |
| CN103881971A (en) | Culture medium and culture method for culturing and/or amplifying mesenchymal stem cells | |
| CN102964152A (en) | Culture medium formula and preparation method of white beech mushroom liquid spawn | |
| CN103030466A (en) | Dendrobium nutrient solution formula for facilitating fast growth and high quality of dendrobium transplanting test-tube plantlets and application of dendrobium nutrient solution formula | |
| KR20080113322A (en) | Induction method of protocorm like body from paphiopedilum young plant and proliferation method thereof | |
| CN104855134A (en) | High-yield cultivation method for auricularia polytricha | |
| CN107439377A (en) | It is a kind of improve Momordica grosvenori can suspend culture frequency of embryonic callus induction method | |
| CN105707901B (en) | Active selenium and its production method | |
| CN104195101B (en) | A kind of Follicular Unit preserves nutrient solution | |
| CN104152401B (en) | A kind of CHMM1 culture mediums suitable for Chinese's epidermal melanophore in vitro culture | |
| CN109777765A (en) | Stem cell media and its application and stem cell culture method | |
| CN106614324A (en) | Cage culture method of table-purpose native chickens | |
| KR20150057052A (en) | A composition for improvement of regeneration system from protcorm-like body of Cymbidium plants and method therefor | |
| CN110692594B (en) | Human hemangioma animal model and high-success-rate construction method thereof | |
| CN105002093B (en) | A kind of marine chlorella culture medium | |
| CN109722407A (en) | A kind of method of microalgae ice startup temperature compensatory growth | |
| CN105230490A (en) | In vitro preservation culture medium for cassava embryogenic callus | |
| CN110484490A (en) | A kind of transplantation method of hair follicle stem cells | |
| CN109097321A (en) | A kind of primary separation method of hair follicle stem cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151230 |