CN105176829A - Application of strain Mariannaeasp. HJ in reduction of Se (IV) to synthesize selenium nanoparticles - Google Patents
Application of strain Mariannaeasp. HJ in reduction of Se (IV) to synthesize selenium nanoparticles Download PDFInfo
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- CN105176829A CN105176829A CN201510180587.3A CN201510180587A CN105176829A CN 105176829 A CN105176829 A CN 105176829A CN 201510180587 A CN201510180587 A CN 201510180587A CN 105176829 A CN105176829 A CN 105176829A
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Abstract
Belonging to the field of biotechnologies, the invention discloses application of a strain Mariannaeasp. HJ in reduction of Se (IV) to synthesize selenium nanoparticles. The strain HJ can reduce 1.0-5.0mmol/L Se (IV) into spherical selenium nanoparticles with a particle size of 130-200nm under a pH of 7-9 and a temperature of 30DEG C. The method for synthesis of selenium nanoparticles with the strain HJ has the characteristics of mild reaction conditions and simple operation, and has potential industrial application prospects.
Description
Technical field
The invention belongs to biological technical field, relate to a strain horse Leah mould
mariannaeasp.HJ synthesizes the application in selenium nano particle in reduction Se (IV).
Technical background
Selenium (Se) is the required one trace element of human body and animal, the multiple biological activity such as have anti-oxidant, immunomodulatory, inhibition tumor cell grows, delay senility.The biomolecules such as selenium nano particle and protein, polysaccharide, lipid combine, and may be used for the production of the materials such as foodstuff additive, VITAMIN, microbiotic, in biological, chemistry, medicine and other fields, have important using value.In addition, selenium nano particle also has unique photoelectric property and characteristic of semiconductor, is widely used in the sealed cells such as solar cell, sensor, rectifier.
The selenium nano particle of current industrial application mainly adopts the technology such as chemistry redox reaction, electrochemistry to produce, but these methods need the conditions such as high temperature, high pressure or catalyzer, and the selenium nano particle biological activity obtained is lower.Research finds, various plants or microorganism all have ability selenate or selenite being reduced to elemental selenium nano particle, comprise algae, bacterium, fungi, actinomycetes etc.Wherein, study with bacterium in the majority, as subtilis
bacillussubtilis, bacillus cereus
bacilluscereus, basophilic pseudomonas
pseudomonasalcaliphila, Shewanella
shewanella, intestinal bacteria
escherichiacolican reduce Se (IV) generate red selenium nano particle Deng, these bacterial strains under aerobic or anaerobic condition.Selenium nano particle is simple to operate, pollution-free, environmental protection to utilize micro-reduction Se (IV) to synthesize, and thus has broad application prospects.
This laboratory separation from the active sludge of iron and steel coke-oven plant, Benxi in early stage obtains a strain horse Leah mould
mariannaeasp.HJ, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 4th, 2014, registers on the books and is numbered CGMCCNo.10030.This laboratory early-stage Study shows horse Leah mould
mariannaeasp.HJ has the ability of synthesis gold nano grain, but rarely has report for the research of horse Leah mould synthesis selenium nano particle at present.
Summary of the invention
The object of the present invention is to provide a kind of method of Microbe synthesis selenium nano material, mainly utilize horse Leah mould
mariannaeasp.HJ reduction Se (IV) synthesizing spherical selenium nano particle.The method reaction conditions is gentle, easy and simple to handle, has potential industrial applications and be worth in biosynthesizing selenium field of nanometer material technology.
Utilize horse Leah mould
mariannaeasp.HJ reduction Se (IV) synthesizes the method for selenium nano particle, and concrete steps are as follows:
(1) from solid improvement Martin substratum, single bacterium colony of picking bacterial strain HJ, in liquid improvement Martin substratum, in 30 DEG C, shaking culture 48h under 150r/min condition, prepares seed culture fluid.Improvement Martin substratum consists of: glucose 10g/L, (NH
4)
2sO
41g/L, MgSO
47H
2o0.5g/L, K
2hPO
41g/L; Solid medium is add 20g/L agar powder in above-mentioned substratum.The substratum of preparation all uses after sterilizing 20min at 115 DEG C.
(2) be connected in liquid improvement Martin substratum according to the inoculum size of volume percent 5% by seed culture fluid, pH is 7 ~ 9, and temperature is 30 DEG C, and Se (IV) concentration is 1.0 ~ 5.0mmol/L, shaking culture 48h under 150r/min condition.
The selenium nano particle prepared by aforesaid method is spherical, and particle diameter is 130 ~ 200nm.
The invention has the beneficial effects as follows: a strain horse Leah mould
mariannaeasp.HJ, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 4th, 2014, registers on the books and is numbered CGMCCNo.10030.Utilize horse Leah mould
mariannaeasp.HJ is that reducible Se (IV) synthesizes selenium nano particle at normal temperatures, and have globosity, particle diameter is 130 ~ 200nm.The method has the features such as reaction conditions gentleness, energy consumption be low, easy and simple to handle, has potential industrial applications prospect in biosynthesizing selenium field of nanometer material technology.
Accompanying drawing explanation
Under Fig. 1 condition of different pH, bacterial strain HJ is to the reduction ratio of Se (IV).
Under Fig. 2 condition of different temperatures, bacterial strain HJ is to the reduction ratio of Se (IV).
Under different Se (IV) concentration conditions of Fig. 3, bacterial strain HJ is to the reduction ratio of Se (IV).
The scanning electron microscope that Fig. 4 bacterial strain HJ synthesizes selenium nano particle characterizes.
The transmission electron microscope that Fig. 5 bacterial strain HJ synthesizes selenium nano particle characterizes.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
embodiment 1:horse Leah mould
mariannaeasp.HJ synthesizes the method for selenium nano particle
Step 1: the preparation of bacterial strain HJ seed culture fluid.From solid improvement Martin substratum, picking list bacterium colony is in liquid improvement Martin substratum, in 30 DEG C, shaking culture 48h under 150r/min condition, prepares seed culture fluid.
Step 2: the preparation of selenium nano particle.Utilize the seed culture fluid that step 1 obtains, be connected in liquid improvement Martin substratum according to the inoculum size of volume percent 5%, pH is 7, and temperature is 30 DEG C, and Se (IV) concentration is 2.0mmol/L, shaking culture 48h under 150r/min condition.
Result can obtain red product in nutrient solution, is the selenium nano particle that bacterial strain HJ reduction Se (IV) generates.
Improvement Martin substratum consists of: glucose 10g/L, (NH
4)
2sO
41g/L, MgSO
40.5g/L, K
2hPO
41g/L; Solid medium is add 20g/L agar powder in above-mentioned substratum.The substratum of preparation all uses after sterilizing 20min at 115 DEG C.
embodiment 2:under condition of different pH, bacterial strain HJ reduction Se (IV) synthesizes selenium nano particle
Step 1 ~ 2 are with embodiment 1, and in regulating step 2, the pH of liquid Martin substratum is respectively 4,5,6,7,8,9.After cultivating 48h, measure the reduction ratio of Se (IV).
Experiment finds (Fig. 1), in the scope that pH is 5 ~ 9, all have red product to generate; Se (IV) reduction ratio when pH is 7 ~ 9 is higher, is about 60%.
embodiment 3:under condition of different temperatures, bacterial strain HJ reduction Se (IV) synthesizes selenium nano particle
Step 1 ~ 2, with embodiment 1, regulate temperature of reaction to be 20,25,30,35,40 DEG C.After cultivating 48h, measure the reduction ratio of Se (IV).
Experiment finds (Fig. 2), in the scope that temperature is 25 ~ 35 DEG C, all have red product to generate; Se (IV) reduction ratio when temperature of reaction is 30 DEG C is the highest, is about 59%.
embodiment 4:under different Se (IV) concentration conditions, bacterial strain HJ synthesizes selenium nano particle
Step 1 ~ 2 with embodiment 1, regulate Se (IV) concentration to be 1.0,2.0,3.0,5.0,7.0mmol/L.After cultivating 48h, measure the reduction ratio of Se (IV).
Experiment finds (Fig. 3), in the scope that Se (IV) concentration is 1.0 ~ 7.0mmol/L, all have red product to generate; Se (IV) reduction ratio when concentration is 1.0mmol/L is the highest, is about 64%; Along with Se (IV) concentration raises, its reduction ratio slightly declines.
embodiment 5:bacterial strain HJ reduction Se (IV) synthesizes the morphology characterization of selenium nano particle
The red product obtained, with embodiment 1, is carried out scanning electron microscope and transmission electron microscope characterizes by step 1 ~ 2, and result as shown in Figure 4 and Figure 5.Horse Leah mould
mariannaeasp.HJ can reduce Se (IV) synthesizing spherical selenium nano particle at cell surface; The nano particle generated is not of uniform size, and particle diameter yardstick is 130 ~ 200nm.
Claims (2)
1. a strain horse Leah mould
mariannaeasp.HJ reduction Se (IV) synthesizes the method for selenium nano particle, it is characterized in that: by horse Leah mould
mariannaeathe bacterial strain of sp.HJ is connected in improvement Martin substratum according to the inoculum size of volume percent 5%, and pH is 7 ~ 9, and temperature is 30 DEG C, and Se (IV) concentration is 1.0 ~ 5.0mmol/L, shaking culture 48h under 150r/min condition; Described improvement Martin substratum consists of: glucose 10g/L, (NH
4)
2sO
41g/L, MgSO
47H
2o0.5g/L, K
2hPO
41g/L.
2. a strain horse Leah mould according to claim 1
mariannaeasp.HJ reduction Se (IV) synthesizes the method for selenium nano particle, it is characterized in that: described horse Leah mould
mariannaeathe bacterial strain of sp.HJ reduces Se (IV) at cell surface and generates spherical selenium nano particle, and the particle diameter of selenium nano particle is 130 ~ 200nm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510180587.3A CN105176829B (en) | 2015-04-17 | 2015-04-17 | Application of one plant of horse Leah mould in reduction Se (IV) synthesis selenium nano particles |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510180587.3A CN105176829B (en) | 2015-04-17 | 2015-04-17 | Application of one plant of horse Leah mould in reduction Se (IV) synthesis selenium nano particles |
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| CN105176829B CN105176829B (en) | 2018-04-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114053298A (en) * | 2021-11-19 | 2022-02-18 | 华中农业大学 | Medicine for inhibiting myocardial hypertrophy and construction method of model |
Citations (2)
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| CN101632453A (en) * | 2009-06-16 | 2010-01-27 | 阳瑞鸿 | Cordycepin film agent |
| WO2013090628A1 (en) * | 2011-12-13 | 2013-06-20 | Synthetic Genomics, Inc. | Plant growth-promoting microbes and uses therefor |
-
2015
- 2015-04-17 CN CN201510180587.3A patent/CN105176829B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101632453A (en) * | 2009-06-16 | 2010-01-27 | 阳瑞鸿 | Cordycepin film agent |
| WO2013090628A1 (en) * | 2011-12-13 | 2013-06-20 | Synthetic Genomics, Inc. | Plant growth-promoting microbes and uses therefor |
Non-Patent Citations (2)
| Title |
|---|
| LEI CAI ET AL.: "Morphological and molecular characterization of Mariannaea aquaticola sp. nov. collected from freshwater habitats", 《MYCOL PROGRESS》 * |
| ZHAO-QING ZENG,WEN-YING ZHUANG: "A new holomorphic species of Mariannaea and epitypification of M. samuelsii", 《MYCOL PROGRESS》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114053298A (en) * | 2021-11-19 | 2022-02-18 | 华中农业大学 | Medicine for inhibiting myocardial hypertrophy and construction method of model |
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