CN103695485A - High-efficiency production method of gamma-polyglutamic acid - Google Patents
High-efficiency production method of gamma-polyglutamic acid Download PDFInfo
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- CN103695485A CN103695485A CN201310749908.8A CN201310749908A CN103695485A CN 103695485 A CN103695485 A CN 103695485A CN 201310749908 A CN201310749908 A CN 201310749908A CN 103695485 A CN103695485 A CN 103695485A
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 26
- 229920002643 polyglutamic acid Polymers 0.000 title abstract description 8
- 238000000855 fermentation Methods 0.000 claims abstract description 29
- 230000004151 fermentation Effects 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 20
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 14
- 238000009423 ventilation Methods 0.000 claims abstract description 13
- 239000001963 growth medium Substances 0.000 claims abstract description 5
- 239000002609 medium Substances 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 18
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 16
- 239000008103 glucose Substances 0.000 claims description 16
- 229940041514 candida albicans extract Drugs 0.000 claims description 11
- 239000012138 yeast extract Substances 0.000 claims description 11
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims description 8
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 8
- 229940073490 sodium glutamate Drugs 0.000 claims description 8
- 230000001133 acceleration Effects 0.000 claims description 5
- 230000009123 feedback regulation Effects 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000011218 seed culture Methods 0.000 claims description 3
- 108010046845 tryptones Proteins 0.000 claims description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract description 10
- 235000013922 glutamic acid Nutrition 0.000 abstract description 10
- 239000004220 glutamic acid Substances 0.000 abstract description 10
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 abstract description 6
- 239000002861 polymer material Substances 0.000 abstract description 3
- 235000010344 sodium nitrate Nutrition 0.000 abstract description 3
- 239000004317 sodium nitrate Substances 0.000 abstract description 3
- 229910002651 NO3 Inorganic materials 0.000 abstract description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 abstract description 2
- 101000681023 Escherichia coli (strain K12) Ribosomal protein S6-L-glutamate ligase Proteins 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 abstract 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 13
- 229960002989 glutamic acid Drugs 0.000 description 10
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 108010020346 Polyglutamic Acid Proteins 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940001516 sodium nitrate Drugs 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000311115 Bacillus paralicheniformis ATCC 9945a Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- WHUUTDBJXJRKMK-GSVOUGTGSA-N D-glutamic acid Chemical compound OC(=O)[C@H](N)CCC(O)=O WHUUTDBJXJRKMK-GSVOUGTGSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a high-efficiency production method of gamma-polyglutamic acid, and belongs to the technical field of production of polymer materials based on biological fermentation. According to the method, bacillus licheniformis CGMCC (China General Microbiological Culture Collection Center) NO.3336 for high-efficient production of gamma-polyglutamic acid is taken as a starting strain, sodium nitrate is added into a fermentation culture medium, and the activity of polyglutamate synthase is influenced under the nitrate respiration condition by adjusting and controlling the pH value and the ventilation quantity in a fermentation process, so that residual glutamic acid in a fermentation solution is reduced and the conversion rate of glutamic acid is increased; after fermentation, the glutamic acid content of the fermentation solution is 5-15 g/L and the gamma-polyglutamic acid yield is 45-55 g/L. Therefore, the economic and feasible production method is provided for industrialization production of gamma-polyglutamic acid.
Description
Technical field
The invention belongs to biological fermentation production technical field of polymer materials, specifically a kind of method of High-efficient Production gamma-polyglutamic acid-.
Background technology
Gamma-polyglutamic acid-is a kind of multifunctional bio degradable high polymer material being combined into by the amido linkage between alpha-amino group and γ-carboxyl by D-Glu and Pidolidone.Because gamma-polyglutamic acid-and derivative thereof have good water-soluble and adsorptivity, can thoroughly be biodegradable, to humans and animals safety non-toxic, the advantage such as edible, thereby can be used as water-holding agent, thickening material, flocculation agent, heavy metal absorbent, medicine, fertilizer slow-release formulation and pharmaceutical carrier etc., at agricultural, food, medicine, makeup, environmental protection, synthon and the field such as film, have broad application prospects.Along with the environmental pollution that chemical industry synthetic materials causes is day by day serious, exploitation Biodegradable material to a certain extent instead of chemical material has become the active demand of whole international community.
The preparation method of polyglutamic acid has chemosynthesis, extraction and three kinds of methods of microorganism fermentation.Microbe fermentation method is lower than the production cost of first two method, and production process is little to the pollution of environment, so mainly adopt now Production by Microorganism Fermentation gamma-polyglutamic acid-.
Both at home and abroad the zymotechnique of gamma-polyglutamic acid-is conducted extensive research, mainly take the culture medium prescription of liquid submerged fermentation and culture process as main.A strain gamma-polyglutamic acid-superior strain Bcillius subtilis NX-2 screens to obtain in Nanjing University of Technology, liquid fermenting is produced to gamma-polyglutamic acid-and done than more comprehensively research, and applied for patent, and patent publication No. is CN1346891.Hua Zhong Agriculture University has also carried out the screening of gamma-polyglutamic acid generating bacterium and the work of process exploitation, and its bacterial classification and liquid production technique have also been applied for patent, and patent publication No. is CN1536071.Korea S researchist adopts stream to add the method for high-density culture to Bacillus licheniformis ATCC9945a in 2.5 liters of fermentor tanks, ferment and after 35 hours, can reach whole output (Yoon SH, DO JH, the Lee SY.Biotechnol.Lett of 35g/L, 2000,22:585-588).Kubota is a separated strain Bacillus subtilis F201 who obtains in Osaka City University soil, this bacterial strain can reach the production peak of 50g/L under best fermentation conditions, this is the production peak (Kubota H.Biosci Biotec Biochem, 1993:1212-1213) of bibliographical information.
It is mainly bacillus that the γ finding at present-PGA produces bacterial strain.According to whether Pidolidone is provided in substratum, bacterial strain can be divided into two large classes: the Pidolidone dependent form of a class for need to add Pidolidone precursor in substratum, another kind of for need to not add the Pidolidone independent form of Pidolidone precursor in substratum.Pidolidone independent form production efficiency is lower; so producing, industrial fermentation substantially all selects Pidolidone dependent form bacterial strain; Pidolidone is added in substratum as important as precursors; but the transformation efficiency of its 50% left and right is also not suitable for economical large-scale production, seek a kind of efficiently, gamma-polyglutamic acid-production method is still a kind of needs cheaply.
Summary of the invention
Technical problem solved by the invention is that to take Bacillus licheniformis (Bacillus licheniformis) the CGMCC NO.3336 of high yield gamma-polyglutamic acid-be starting strain, to add SODIUMNITRATE in fermention medium and by pH value and ventilation in regulation and control fermenting process with the residue of minimizing fermented liquid Glutamic Acid, improve the transformation efficiency of L-glutamic acid, to reduce costs, improve the output of gamma-polyglutamic acid-.
The bacterial strain of production high molecular gamma-polyglutamic acid-provided by the invention is specially Bacillus licheniformis (Bacillus licheniformis) CGMCC NO.3336.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on October 14th, 2009 and (is called for short CGMCC, address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101), preserving number is CGMCC NO.3336, and Classification And Nomenclature is: Bacillus licheniformis (Bacillus licheniformis).
A method for High-efficient Production gamma-polyglutamic acid-, comprises the steps:
(1) actication of culture: Bacillus licheniformis CGMCC NO.3336 original strain is seeded on slant medium, in 37 ℃ of cultivations 16 hours, so activates 2-3 time, prepare ripe inclined-plane seed;
Described slant medium consists of: peptone 10g/L, and yeast powder 5g/L, NaCl10g/L, agar 20g/L, pH7.2, insufficient section pure water is supplied;
(2) preparation of seed liquor: the inclined-plane seed after a ring activation is inoculated in the 500ml triangular flask that 50ml liquid nutrient medium is housed, and 37 ℃, 220rpm cultivates 16 hours to logarithmic phase;
Described seed culture medium consists of: glucose 30g/L, yeast extract paste 7g/L, Tryptones 10g/L, K
2hPO
40.5g/L, MgSO
47H
2o0.5g/L, pH7.2, insufficient section pure water is supplied;
(3) ferment tank: the seed liquor in step (2) is inoculated in to fermentor tank with 10% inoculum size, 30L ferment tank substratum liquid amount is 15L, 37 ℃ of initial leavening temperatures, tank pressure 0.01-0.05Mpa, ventilation 0.5-1.0vvm, rotating speed 400-600rpm; When fermenting to adjusting rotary speed after 24h to 200-400rpm, ventilation 1.0-1.5vvm, and start to add fed-batch medium, the flow acceleration of the feedback regulation fed-batch medium by pH value, control pH value at 7.4-7.9 to 2-6 hour before fermentation ends, total fermentation time is 72h.
Described fermention medium consists of: glucose 80g/L, yeast extract paste 20g/L, NH
4nO
34.5g/L, MgSO
46H
2o0.5g/L, NaCl10g/L, CaCl
26H
2o0.5g/L, FeSO
40.01g/L, Sodium Glutamate 40g/L, NaNO
320-40g/L, MnSO
4h
2o0.15g/L, pH value 7.2-7.5, insufficient section pure water is supplied;
Described fed-batch medium consists of: glucose 650-850g/L, NaCl30.0-60.0g/L, CaCl
26H
2o10-15.0g/L, MnSO
4h
2o5-10g/L, MgSO
46H
2o5-20g/L, pH value 7.4-7.9, insufficient section pure water is supplied;
The centrifugal thalline that goes after fermentation ends, gets 100 times of distilled water dilutings for supernatant liquor, by SBA-40E bio-sensing instrument, residue L-glutamic acid is detected, and content of glutamic acid is 5-15g/L, gamma-polyglutamic acid-output 45-55g/L.
Beneficial effect:
It is starting strain that Bacillus licheniformis (Bacillus licheniformis) the CGMCC NO.3336 of high yield gamma-polyglutamic acid-is take in the present invention, in fermention medium, add SODIUMNITRATE and pass through pH value and ventilation in regulation and control fermenting process, under nitrate respiration condition, affect the activity of polyglutamic acid synthetic enzyme, thereby reduce the residue of fermented liquid Glutamic Acid, improve the transformation efficiency of L-glutamic acid, fermentation ends secondary fermentation liquid Glutamic Acid content is 5-15g/L, gamma-polyglutamic acid-output 45-55g/L.For gamma-polyglutamic acid-suitability for industrialized production provides a kind of economically viable production method.
Embodiment
Below by specific embodiment narration the present invention.Unless stated otherwise, in the present invention, technique means used is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, do not deviating under the prerequisite of essence of the present invention and scope various changes that the material component in these embodiments and consumption are carried out or change and also belong to protection scope of the present invention.
Embodiment 1
A method for High-efficient Production gamma-polyglutamic acid-, comprises the steps:
(1) actication of culture: Bacillus licheniformis CGMCC NO.3336 original strain is seeded on slant medium, in 37 ℃ of cultivations 16 hours, so activates 2 times, prepare ripe inclined-plane seed;
Described slant medium consists of: peptone 10g/L, and yeast powder 5g/L, NaCl10g/L, agar 20g/L, pH7.2, insufficient section pure water is supplied;
(2) preparation of seed liquor: the inclined-plane seed after a ring activation is inoculated in the 500ml triangular flask that 50ml liquid nutrient medium is housed, and 37 ℃, 220rpm cultivates 16 hours to logarithmic phase;
Described seed culture medium consists of: glucose 30g/L, yeast extract paste 7g/L, Tryptones 10g/L, K
2hPO
40.5g/L, MgSO
47H
2o0.5g/L, pH7.2, insufficient section pure water is supplied;
(3) ferment tank: the seed liquor in step (2) is inoculated in to fermentor tank with 10% inoculum size, and 30L ferment tank substratum liquid amount is 15L, 37 ℃ of initial leavening temperatures, tank pressure 0.03Mpa ventilation 0.8vvm, rotating speed 500rpm; When fermenting to adjusting rotary speed after 24h to 300rpm, ventilation 1.2vvm, and start to add fed-batch medium, the flow acceleration of the feedback regulation fed-batch medium by pH value, controls pH value at 7.7 to fermentation ends first 4 hours, and total fermentation time is 72h.
Described fermention medium consists of: glucose 80g/L, yeast extract paste 20g/L, NH
4nO
34.5g/L, MgSO
46H
2o0.5g/L, NaCl10g/L, CaCl
26H
2o0.5g/L, FeSO
40.01g/L, Sodium Glutamate 40g/L, NaNO
330g/L, MnSO
4h
2o0.15g/L, pH value 7.2, insufficient section pure water is supplied;
Described fed-batch medium consists of: glucose 750g/L, NaCl40g/L, CaCl
26H
2o12g/L, MnSO
4h
2o8g/L, MgSO
46H
2o12g/L, pH value 7.7, insufficient section pure water is supplied;
Fermentation ends is by detection, and fermented liquid Glutamic Acid content is 10g/L, gamma-polyglutamic acid-output 50g/L.
Embodiment 2
Actication of culture and seed liquor preparation are with embodiment 1
(3) ferment tank: the seed liquor in step (2) is inoculated in to fermentor tank with 10% inoculum size, and 30L ferment tank substratum liquid amount is 15L, 37 ℃ of initial leavening temperatures, tank pressure 0.01Mpa ventilation 0.5vvm, rotating speed 400rpm; When fermenting to adjusting rotary speed after 24h to 200rpm, ventilation 1.0vvm, and start to add fed-batch medium, the flow acceleration of the feedback regulation fed-batch medium by pH value, controls pH value at 7.4 to fermentation ends first 2 hours, and total fermentation time is 72h.
Described fermention medium consists of: glucose 80g/L, yeast extract paste 20g/L, NH4NO
34.5g/L, MgSO
46H
2o0.5g/L, NaCl10g/L, CaCl
26H
2o0.5g/L, FeSO
40.01g/L, Sodium Glutamate 40g/L, NaNO
320g/L, MnSO
4h
2o0.15g/L, pH value 7.4, insufficient section pure water is supplied;
Described fed-batch medium consists of: glucose 650g/L, NaCl30.0g/L, CaCl
26H
2o10g/L, MnSO
4h
2o5g/L, MgSO
46H
2o5g/L, pH value 7.4, insufficient section pure water is supplied;
Fermentation ends is by detection, and fermented liquid Glutamic Acid content is 15g/L, gamma-polyglutamic acid-output 45g/L.
Embodiment 3
(3) ferment tank: the seed liquor in step (2) is inoculated in to fermentor tank with 10% inoculum size, and 30L ferment tank substratum liquid amount is 15L, 37 ℃ of initial leavening temperatures, tank pressure 0.05Mpa ventilation 1.0vvm, rotating speed 600rpm; When fermenting to adjusting rotary speed after 24h to 400rpm, ventilation 1.5vvm, and start to add fed-batch medium, the flow acceleration of the feedback regulation fed-batch medium by pH value, controls pH value at 7.9 to fermentation ends first 6 hours, and total fermentation time is 72h.
Described fermention medium consists of: glucose 80g/L, yeast extract paste 20g/L, NH
4nO
34.5g/L, MgSO
46H
2o0.5g/L, NaCl10g/L, CaCl
26H
2o0.5g/L, FeSO
40.01g/L, Sodium Glutamate 40g/L, NaNO
340g/L, MnSO
4h
2o0.15g/L, pH value 7.5, insufficient section pure water is supplied;
Described fed-batch medium consists of: glucose 850g/L, sodium-chlor 60.0g/L, CaCl
26H
2o15.0g/L, MnSO
4h
2o10g/L, MgSO
46H
2o20g/L, pH value 7.9, insufficient section pure water is supplied;
Fermentation ends is by detection, and fermented liquid Glutamic Acid content is 5g/L, gamma-polyglutamic acid-output 55g/L.
Claims (4)
1. a method for High-efficient Production gamma-polyglutamic acid-, is characterized in that: Bacillus licheniformis (Bacillus licheniformis) the CGMCC NO.3336 of take is starting strain, by liquid submerged fermentation, prepares, and comprises the steps:
(1) actication of culture: Bacillus licheniformis CGMCC NO.3336 original strain is seeded on slant medium, in 37 ℃ of cultivations 16 hours, so activates 2-3 time, prepare ripe inclined-plane seed;
Described slant medium consists of: peptone 10g/L, and yeast powder 5g/L, NaCl10g/L, agar 20g/L, pH7.2, insufficient section pure water is supplied;
(2) preparation of seed liquor: the inclined-plane seed after a ring activation is inoculated in the 500ml triangular flask that 50ml liquid nutrient medium is housed, and 37 ℃, 220rpm cultivates 16 hours to logarithmic phase;
Described seed culture medium consists of: glucose 30g/L, yeast extract paste 7g/L, Tryptones 10g/L, K
2hPO
40.5g/L, MgSO
47H
2o0.5g/L, pH7.2, insufficient section pure water is supplied;
(3) ferment tank: the seed liquor in step (2) is inoculated in to fermentor tank with 10% inoculum size, 30L ferment tank substratum liquid amount is 15L, 37 ℃ of initial leavening temperatures, tank pressure 0.01-0.05Mpa, ventilation 0.5-1.0vvm, rotating speed 400-600rpm; When fermenting to adjusting rotary speed after 24h to 200-400rpm, ventilation 1.0-1.5vvm, and start to add fed-batch medium, the flow acceleration of the feedback regulation fed-batch medium by pH value, control pH value at 7.4-7.9 to 2-6 hour before fermentation ends, total fermentation time is 72h;
Described fermention medium consists of: glucose 80g/L, yeast extract paste 20g/L, NH
4nO
34.5g/L, MgSO
46H
2o0.5g/L, NaCl10g/L, CaCl
26H
2o0.5g/L, FeSO
40.01g/L, Sodium Glutamate 40g/L, NaNO
320-40g/L, MnSO
4h
2o0.15g/L, pH value 7.2-7.5, insufficient section pure water is supplied;
Described fed-batch medium consists of: glucose 650-850g/L, NaCl30.0-60.0g/L, CaCl
26H
2o10-15.0g/L, MnSO
4h
2o5-10g/L, MgSO
46H
2o5-20g/L, pH value 7.4-7.9, insufficient section pure water is supplied.
2. the method for a kind of High-efficient Production gamma-polyglutamic acid-as claimed in claim 1, is characterized in that: described fermention medium consists of: glucose 80g/L, yeast extract paste 20g/L, NH
4nO
34.5g/L, MgSO
46H
2o0.5g/L, NaCl10g/L, CaCl
26H
2o0.5g/L, FeSO
40.01g/L, Sodium Glutamate 40g/L, NaNO
330g/L, MnSO
4h
2o0.15g/L, pH value 7.2, insufficient section pure water is supplied.
3. the method for a kind of High-efficient Production gamma-polyglutamic acid-as claimed in claim 1, is characterized in that: described fermention medium consists of: glucose 80g/L, yeast extract paste 20g/L, NH
4nO
34.5g/L, MgSO
46H
2o0.5g/L, NaCl10g/L, CaCl
26H
2o0.5g/L, FeSO
40.01g/L, Sodium Glutamate 40g/L, NaNO
320g/L, MnSO
4h
2o0.15g/L, pH value 7.4, insufficient section pure water is supplied.
4. the method for a kind of High-efficient Production gamma-polyglutamic acid-as claimed in claim 1, is characterized in that: described fermention medium consists of: glucose 80g/L, yeast extract paste 20g/L, NH
4nO
34.5g/L, MgSO
46H
2o0.5g/L, NaCl10g/L, CaCl
26H
2o0.5g/L, FeSO
40.01g/L, Sodium Glutamate 40g/L, NaNO
340g/L, MnSO
4h
2o0.15g/L, pH value 7.5, insufficient section pure water is supplied.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017760A (en) * | 2014-06-10 | 2014-09-03 | 河南农业大学 | Application of gamma-polyglutamic acid producing strain and fermentation product thereof |
| CN104694437A (en) * | 2015-03-23 | 2015-06-10 | 领先生物农业股份有限公司 | Bacillus licheniformis and application of bacillus licheniformis in gamma-polyglutamic acid production |
| CN106282252A (en) * | 2015-06-01 | 2017-01-04 | 山东建筑大学 | A kind of high density fermentation prepares culture medium and the fermentation process thereof of gamma-polyglutamic acid- |
| CN106591190A (en) * | 2016-12-16 | 2017-04-26 | 大连理工大学 | Bacillus and application in preparing Gama-polyglutamic acid |
| CN107699594A (en) * | 2017-11-22 | 2018-02-16 | 天津北洋百川生物技术有限公司 | A kind of method that γ polyglutamic acids are produced using bacillus licheniformis |
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| CN109182404A (en) * | 2018-10-15 | 2019-01-11 | 天津科技大学 | A method of utilizing stream plus sugar industry gamma-polyglutamic acid when the lichen bacillus ferments |
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| CN104017760A (en) * | 2014-06-10 | 2014-09-03 | 河南农业大学 | Application of gamma-polyglutamic acid producing strain and fermentation product thereof |
| CN104694437A (en) * | 2015-03-23 | 2015-06-10 | 领先生物农业股份有限公司 | Bacillus licheniformis and application of bacillus licheniformis in gamma-polyglutamic acid production |
| CN104694437B (en) * | 2015-03-23 | 2018-04-27 | 领先生物农业股份有限公司 | One bacillus licheniformis and its purposes in gamma-polyglutamic acid is produced |
| CN106282252A (en) * | 2015-06-01 | 2017-01-04 | 山东建筑大学 | A kind of high density fermentation prepares culture medium and the fermentation process thereof of gamma-polyglutamic acid- |
| CN106591190A (en) * | 2016-12-16 | 2017-04-26 | 大连理工大学 | Bacillus and application in preparing Gama-polyglutamic acid |
| CN107699594A (en) * | 2017-11-22 | 2018-02-16 | 天津北洋百川生物技术有限公司 | A kind of method that γ polyglutamic acids are produced using bacillus licheniformis |
| CN109161567A (en) * | 2018-10-09 | 2019-01-08 | 四川省食品发酵工业研究设计院 | A method of improving gamma-polyglutamic acid yield |
| CN109182404A (en) * | 2018-10-15 | 2019-01-11 | 天津科技大学 | A method of utilizing stream plus sugar industry gamma-polyglutamic acid when the lichen bacillus ferments |
| CN109988788A (en) * | 2019-04-24 | 2019-07-09 | 武汉骏安生物科技有限公司 | A method of promoting bacillus licheniformis high yield polyglutamic acid sodium |
| CN109988788B (en) * | 2019-04-24 | 2022-11-15 | 武汉骏安生物科技有限公司 | Method for promoting high yield of sodium polyglutamate by bacillus licheniformis |
| CN114456980A (en) * | 2022-02-28 | 2022-05-10 | 天津科技大学 | Gamma-polyglutamic acid high-yield strain and application thereof |
| CN114456980B (en) * | 2022-02-28 | 2023-08-11 | 天津北洋百川生物技术有限公司 | Gamma-polyglutamic acid high-yield strain and application thereof |
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