CN105168336A - Natural medicine composition for adjusting diabetes immune and metabolism functions and preparation method of natural medicine composition - Google Patents
Natural medicine composition for adjusting diabetes immune and metabolism functions and preparation method of natural medicine composition Download PDFInfo
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Abstract
The invention discloses natural medicine composition for adjusting diabetes immune and metabolism functions and a preparation method of the natural medicine composition. The natural medicine composition comprises raw materials as follows: cordyceps militaris, cyclocarya paliurus leaves and ginkgo biloba extract. A pharmacology experiment proves that the level of TG (triacylglycerol), TC (total cholesterol) and LDL-C (low-density lipoprotein cholesterol) in serum of a db/db diabetic mouse can be remarkably reduced and indicates that a product researched by our company has a remarkable function of adjustment of fat metabolism and can effectively protect the liver and the kidneys. According to compatibility of medicines, immunity declining and glucolipid metabolism disorder caused by diabetes are taken as pointcut, the functions of immunity boosting and metabolic regulation of cordyceps militaris, cyclocarya paliurus and the ginkgo leaves are used for research of health-care food, and a certain scientific theoretical foundation is provided.
Description
Technical field
The present invention relates to a kind of natural drug composition and the preparation method that regulate diabetes immunity metabolic function, belong to field of health care food.
Background technology
Diabetes (DM) are the decompensated diseases of one group of general, its pathogenic factor be insulin secretion definitely or relative deficiency (defect of insulin secretion), and body target tissue or target organ reduce (insulin action defect) to insulin sensitivity, have I type and II type point.Diabetics often causes a series of immune inflammation due to blood glucose, dyslipidemia, cause body's immunity decline, lower for the resistance, from then on vicious cycle.Along with the progress of growth in the living standard, aged tendency of population and living-pattern preservation and diagnostic techniques, the sickness rate of diabetes is rising year by year, and at present clinically diabetes also cannot effect a radical cure, needs of patients long-term prescription.The EPDML survey result of diabetes is announced according to diabetology branch of Chinese Medical Association in 2013, China's more than 30 years old crowd's diabetes prevalence reaches 11.6%, estimate that there are 1.39 hundred million diabeticss in the whole nation, and diabetic complication serious harm human health and quality of life.Therefore, developing of active intervention diabetes and complication thereof, has become the problem that society is in the urgent need to address.
Current diabetes mellitus prevention and treatment mainly concentrate on chemotherapy and dietetic therapy two aspects.Chemotherapy needs long-term prescription, toxic and side effects many.Although the dietetic therapy conditioning cycle is long, take effect slow but safety is good, and has Nutrition concurrently, the auxiliary treatment for diabetes is very useful.
Summary of the invention
The object of the invention is to: provide a kind of proportioning scientific and reasonable, determined curative effect, the improvement blood sugar in diabetic patients blood fat had no adverse reaction and the natural drug composition of immunity, and the preparation method of said composition.
The object of the present invention is achieved like this: with Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja, Folium Ginkgo extract for main material, preparation method for by Cordyceps militaris (L.) Link., the drying of leaf of Cyclocarya paliurus Iljinskaja water extraction is concentrated beat powder after sieve respectively, Folium Ginkgo extract directly buys and namely uses, mix according to a certain ratio, make the dosage form health foods such as powdery, graininess, lamellar, capsule.
The invention provides a kind of natural drug composition regulating diabetes immunity metabolic function, be made up of the raw material of following weight proportion: Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja, Folium Ginkgo extract.
The natural drug composition of described adjustment diabetes immunity metabolic function, is made up of the raw material of following weight proportion: Cordyceps militaris (L.) Link. 40-55 part, leaf of Cyclocarya paliurus Iljinskaja 35-55 part, Folium Ginkgo extract 4-8 part.
The natural drug composition of described adjustment diabetes immunity metabolic function, is made up of the raw material of following weight proportion: Cordyceps militaris (L.) Link. 50 parts, leaf of Cyclocarya paliurus Iljinskaja 40 parts, Folium Ginkgo extract 6 parts.
The natural drug composition of described adjustment diabetes immunity metabolic function, dosage form is any one in the other forms of dosage forms such as tablet in oral formulations, hard capsule, soft capsule, granule, powder, pill.
The natural drug composition of described adjustment diabetes immunity metabolic function, preparation method comprises the following steps: (1) leaf of Cyclocarya paliurus Iljinskaja process: leaf of Cyclocarya paliurus Iljinskaja is clean, dry, add the water of 15-30 times amount, decoct after soaking 30-60min, adjust the temperature to micro-boiling after boiling, after keeping slight boiling condition 60min, collect filtrate; The remaining residue of institute take water as solvent, and the water adding 10-15 times amount continues to decoct, and adjusts the temperature to slight boiling condition after boiling, collects filtrate, repeat this step twice after keeping slight boiling condition 60min; Merge three filtrates, use heating in water bath when carrying out being concentrated into 60 DEG C the relative density of thick paste be 1.15-1.20, after carry out drying under reduced pressure, condition is 60 DEG C, and-0.06MPa, obtains dry extract; Dry gained dry extract is put into pulverizer to pulverize, cross 100 mesh sieves and obtain leaf of Cyclocarya paliurus Iljinskaja extract, discharging, for subsequent use; (2) Cordyceps militaris (L.) Link. process: Cordyceps militaris (L.) Link. is put into pulverizer and pulverize, cross 100 mesh sieves, discharging, for subsequent use; (3) Folium Ginkgo extract; (4) batch mixing: by the dry powder for subsequent use of (1), (2), (3) gained according to recipe requirements accurate weighing, put into mixer, mix homogeneously, obtain pulverulent mixture for subsequent use, makes various dosage form.
Folium Ginkgo extract, purchased from Jianfeng Natural Product R&D Development Co., Ltd., Tianjin, meets standards of pharmacopoeia.Cyclocarya paliurus Iljinskaja (latin name: Cyclocaryapaliurus (Batal.) Iljin. [PterocaryapaliurusBatal.; P.micropaliurusTsoong], FoliumCylocaryaePaliuri) leaf, Cyclocarya paliurus Iljinskaja is money tree, numb willow again, Juglandaceae.Cordyceps militaris (L.) Link. is Ascomycotina, Ergota Zoopagales, the type sepecies of Clavicipitaceae, Cordyceps.Formal name used at school is Cordycepsmilitaris, has another name called Cordyceps militaris (L.) Link., Cordyceps militaris etc.
Beneficial effect:
1, the present inventor rest on a scientific basis theory, through repetition test, have chosen safety, Cordyceps militaris (L.) Link. without any side effects, leaf of Cyclocarya paliurus Iljinskaja, Folium Ginkgo three kinds of raw materials can regulate diabetes immunity metabolic function health food in order to prepare the present invention.Choosing Cordyceps militaris (L.) Link. is because it is mainly containing cordycepin, cordycepic acid, Cordyceps polysaccharide, SOD isoreactivity material, can improve diabetics immunity, improve glucose-lipid metabolism, and " Xinhua's book on Chinese herbal medicine outline " is recorded: Cordyceps militaris (L.) Link. sweet in the mouth, property is put down.There is lung benefiting kidney, mend marrow, the function of hemostasis and phlegm.Theory of Chinese medical science thinks that Cordyceps enters lung kidney two warp, can tonifying the lung cloudy, again can kidney-replenishing, cure mainly suffer from a deficiency of the kidney, impotence and seminal emission, soreness of waist and knee joint, after being ill weak, chronic cough is weak, phthisical cough expectorant blood, spontaneous sweating etc., is unique a kind of Chinese medicine that can balance simultaneously, regulate negative and positive.And select leaf of Cyclocarya paliurus Iljinskaja to be because leaf of Cyclocarya paliurus Iljinskaja main chemical compositions is flavonoid, triterpenes, saponin, sterol, organic acid, alkaloid and polysaccharide etc., its polysaccharide, flavonoid and trace element have good blood sugar lowering, the effect of blood fat, simultaneously to enhancing body immunity, antioxidation, defying age, protect the liver fatty liver and there is unique effect.Theory of Chinese medical science thinks that leaf of Cyclocarya paliurus Iljinskaja is warm in nature, and the micro-hardship of acrid in the mouth, has clearing heat for detumescence, removing toxic substances, analgesic efficacy.In formula, add Folium Ginkgo is because it contains multiple flavonoid, terpenoid, phenols, polysaccharide and various trace element and aminoacid simultaneously, not only blood sugar for human body can be reduced, be significantly improved effect to blood lipid metabolism, also can promote islet β cell, insulin increases.Theory of Chinese medical science thinks this mild in medicine property, bitter in the mouth, puckery, and having astringes the lung relievings asthma, effect of antidiarrheal, promoting blood circulation and stopping pain.Because Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja, Folium Ginkgo all have the effect of scavenger interior free yl, can synergism be played, strengthen the ability directly or indirectly removing interior free yl further, reach effect of harmful material in comprehensive and systematic removing body; Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja, Folium Ginkgo all can improve metabolism of blood glucose, reduce blood fat, improve immunity simultaneously, and Cordyceps militaris (L.) Link. can also repair impaired beta Cell of islet, Folium Ginkgo can promote the secretion of insulin, and comprehensive use can be played good adjusting blood lipid, metabolism of blood glucose effect to diabetics and improve the effect of immunity.Theory of Chinese medical science is thought, Cordyceps militaris (L.) Link., Folium Ginkgo have played lung benefiting kidney to kidney, lung, liver, have mended marrow, gas of astringing the lung, enter effect of lung Liver Channel, add that leaf of Cyclocarya paliurus Iljinskaja has effect of clearing heat for detumescence, removing toxic substances, Zhu Pin share, and can play the merit of supplementing QI and nourishing YIN, blood circulation promoting and blood stasis dispelling, the turbid blood fat reducing of change.So three kinds of compatibilities are combined in this formula, can mutually promote, synergism, reaches adjusting blood lipid metabolism, improves the object of immunity.
2, the present invention adopts Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja, Folium Ginkgo science formulated, its formula is reasonable, the comprehensive use of each component, can play and remove oxygen-derived free radicals in body and other harmful substances, play comprehensively, adjustment blood glucose, the blood fat of system and improve immunity.Not only be suitable for blood fat, high blood glucose crowd, more can play good prevention effect to diabetes and hyperlipemia.And the raw material of health food adopts natural prodcuts in the present invention, be a kind of safety, completely without any side effects, can the natural health care of long-term taking.
3, current existing treatment and improve diabetes health food mostly for single function of reducing blood sugar, or formula lacks the defect such as pathogenesis for diabetes complexity.The present invention is directed to the phenomenon such as immune disorder, Metabolic disorder, circulatory disturbance in onset diabetes generation evolution, by comprehensive functions such as Cordyceps militaris (L.) Link. improves the immune disorder of diabetes, Folium Ginkgo improves diabetes circulatory disturbance, Cyclocarya paliurus Iljinskaja repairing islet cells and functions, jointly reach the generation or development of intervening diabetic complication.The present invention uses leaf of Cyclocarya paliurus Iljinskaja, Cordyceps militaris (L.) Link., Folium Ginkgo for main formula, and above raw material is made powdery, graininess, lamellar, capsule health-care food, is used for the treatment of the immune Metabolic disorder that diabetes cause.The present invention compares with existing patent of invention, except make use of modern pharmacology achievement in research, more outstanding according to pathogenesis of diabetes mellitus, and its intervention means of reverse research, solves the metabolism immune disorder problem that diabetes cause.Formula is reasonable, and dosage form is advanced, has science, the advantage such as economic, practical.
Detailed description of the invention
Embodiment 1: tablet
Tablet producing technology is as follows:
(1) leaf of Cyclocarya paliurus Iljinskaja extract preparation
Leaf of Cyclocarya paliurus Iljinskaja 40g is clean, dry, be solvent with water, add the water of 24 times amount, decoct after soaking 40min, after boiling, adjust the temperature to micro-boiling, after keeping slight boiling condition 60min, collect filtrate; The remaining residue of institute take water as solvent, and the water adding 12 times amount continues to decoct, and adjusts the temperature to slight boiling condition after boiling, collects filtrate, repeat this step twice after keeping slight boiling condition 60min; Merge three filtrates, after using heating in water bath to carry out being concentrated into thick paste (1.15-1.20,60 DEG C), carry out (60 DEG C ,-0.06MPa) drying under reduced pressure; Drying is got dry extract and puts into pulverizer pulverizing, cross 100 mesh sieves and obtain leaf of Cyclocarya paliurus Iljinskaja extract, discharging, for subsequent use.
(2) Cordyceps militaris (L.) Link. process
Cordyceps militaris (L.) Link. 50g is put into pulverizer to pulverize, cross 100 mesh sieves, discharging, for subsequent use.
(3) Folium Ginkgo extract
Folium Ginkgo extract 6g, namely direct purchase is used (purchased from Jianfeng Natural Product R&D Development Co., Ltd., Tianjin).
(4) batch mixing
Take Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja, the Folium Ginkgo extract prepared with said method, put into mixer, mix homogeneously, sieve, obtain pulverulent mixture for subsequent use.
(5) granulate
The dry powder of above-mentioned mix homogeneously is aided with appropriate adjuvant, puts into granulator and add the ethanol mixed pelletization that the purity being equivalent to dry powder gross weight 20% is 90%.
(6) dry granulate
Being placed in baking oven by obtained granule, carrying out drying with 60 DEG C, is 3% to moisture; First use 24 mesh sieve granulate, then with 100 mesh sieve screenings by the granule by 24 mesh sieves, the granule not by 100 mesh sieves is for subsequent use, and form graininess, underproof granule reclaims.
(7) tabletting
Pour tablet machine hopper compress tablet coating after the appropriate moistening of granule step 6 prepared into, select suitable machine parameter to run, get 10 every 15min and carry out weight differential detection, form tablet health food.
Embodiment 2: tablet
Tablet producing technology is as follows:
(1) leaf of Cyclocarya paliurus Iljinskaja extract preparation
Leaf of Cyclocarya paliurus Iljinskaja 35g is clean, dry, be solvent with water, add the water of 20 times amount, decoct after soaking 40min, after boiling, adjust the temperature to micro-boiling, after keeping slight boiling condition 60min, collect filtrate; The remaining residue of institute take water as solvent, and the water adding 12 times amount continues to decoct, and adjusts the temperature to slight boiling condition after boiling, collects filtrate, repeat this step twice after keeping slight boiling condition 60min; Merge three filtrates, after using heating in water bath to carry out being concentrated into thick paste (1.15-1.20,60 DEG C), carry out (60 DEG C ,-0.06MPa) drying under reduced pressure; Drying is got dry extract and puts into pulverizer pulverizing, cross 100 mesh sieves and obtain leaf of Cyclocarya paliurus Iljinskaja extract, discharging, for subsequent use.
(2) Cordyceps militaris (L.) Link. process
Cordyceps militaris (L.) Link. 55g is put into pulverizer to pulverize, cross 100 mesh sieves, discharging, for subsequent use.
(3) Folium Ginkgo extract
Folium Ginkgo extract 8g, namely direct purchase is used (purchased from Jianfeng Natural Product R&D Development Co., Ltd., Tianjin).
(4) batch mixing
Take Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja extract, the Folium Ginkgo extract prepared with said method, put into mixer, mix homogeneously, sieve, obtain pulverulent mixture for subsequent use.
(5) granulate
The dry powder of above-mentioned mix homogeneously is aided with appropriate adjuvant, puts into granulator and add the ethanol mixed pelletization that the purity being equivalent to dry powder gross weight 20% is 90%.
(6) dry granulate
Being placed in baking oven by obtained granule, carrying out drying with 60 DEG C, is 3% to moisture; First use 24 mesh sieve granulate, then with 100 mesh sieve screenings by the granule by 24 mesh sieves, the granule not by 100 mesh sieves is for subsequent use, and form graininess, underproof granule reclaims.
(7) tabletting
Pour tablet machine hopper compress tablet coating after the appropriate moistening of granule step 6 prepared into, select suitable machine parameter to run, get 10 every 15min and carry out weight differential detection, form tablet health food.
Embodiment 3: capsule
Capsule preparation technology is as follows:
(1) leaf of Cyclocarya paliurus Iljinskaja extract preparation
Leaf of Cyclocarya paliurus Iljinskaja 55g part is clean, dry, be solvent with water, add the water of 24 times amount, decoct after soaking 40min, after boiling, adjust the temperature to micro-boiling, after keeping slight boiling condition 60min, collect filtrate; The remaining residue of institute take water as solvent, and the water adding 12 times amount continues to decoct, and adjusts the temperature to slight boiling condition after boiling, collects filtrate, repeat this step twice after keeping slight boiling condition 60min; Merge three filtrates, after using heating in water bath to carry out being concentrated into thick paste (1.15-1.20,60 DEG C), carry out (60 DEG C ,-0.06MPa) drying under reduced pressure; Drying is got dry extract and puts into pulverizer pulverizing, cross 100 mesh sieves and obtain leaf of Cyclocarya paliurus Iljinskaja extract, discharging, for subsequent use.
(2) Cordyceps militaris (L.) Link. process
Cordyceps militaris (L.) Link. 40g is put into pulverizer to pulverize, cross 100 mesh sieves, discharging, for subsequent use.
(3) Folium Ginkgo extract
Folium Ginkgo extract 4g, namely direct purchase is used (purchased from Jianfeng Natural Product R&D Development Co., Ltd., Tianjin).
(4) batch mixing
Take Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja extract, the Folium Ginkgo extract prepared with said method, put into mixer, mix homogeneously, sieve, obtain pulverulent mixture for subsequent use.
(5) granulate
The dry powder of above-mentioned mix homogeneously is aided with appropriate adjuvant, puts into granulator and add the ethanol mixed pelletization that the purity being equivalent to dry powder gross weight 20% is 90%.
(6) dry granulate
Being placed in baking oven by obtained granule, carrying out drying with 60 DEG C, is 3% to moisture; First use 24 mesh sieve granulate, then with 100 mesh sieve screenings by the granule by 24 mesh sieves, the granule not by 100 mesh sieves is for subsequent use, and form graininess, underproof granule reclaims.
(7) fill
Granule step 6 prepared uses capsule filling machine, selects suitable machine parameter to run, makes capsule.
Embodiment 4: granule
Preparation Technology of Granules is as follows:
(1) leaf of Cyclocarya paliurus Iljinskaja extract preparation
Leaf of Cyclocarya paliurus Iljinskaja 40g is clean, dry, be solvent with water, add the water of 24 times amount, decoct after soaking 40min, after boiling, adjust the temperature to micro-boiling, after keeping slight boiling condition 60min, collect filtrate; The remaining residue of institute take water as solvent, and the water adding 12 times amount continues to decoct, and adjusts the temperature to slight boiling condition after boiling, collects filtrate, repeat this step twice after keeping slight boiling condition 60min; Merge three filtrates, after using heating in water bath to carry out being concentrated into thick paste (1.15-1.20,60 DEG C), carry out (60 DEG C ,-0.06MPa) drying under reduced pressure; Drying is got dry extract and puts into pulverizer pulverizing, cross 100 mesh sieves and obtain leaf of Cyclocarya paliurus Iljinskaja extract, discharging, for subsequent use.
(2) Cordyceps militaris (L.) Link. process
Cordyceps militaris (L.) Link. 50g is put into pulverizer to pulverize, cross 100 mesh sieves, discharging, for subsequent use.
(3) Folium Ginkgo extract
Folium Ginkgo extract 6g, namely direct purchase is used (purchased from Jianfeng Natural Product R&D Development Co., Ltd., Tianjin).
(4) batch mixing
Take Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja extract, the Folium Ginkgo extract prepared with said method, put into mixer, mix homogeneously, sieve, obtain pulverulent mixture for subsequent use.
(5) granulate
The dry powder of above-mentioned mix homogeneously is aided with appropriate adjuvant, puts into granulator and add the ethanol mixed pelletization that the purity being equivalent to dry powder gross weight 20% is 90%.
(6) dry granulate
Being placed in baking oven by obtained granule, carrying out drying with 60 DEG C, is 3% to moisture; First use 24 mesh sieve granulate, then with 100 mesh sieve screenings by the granule by 24 mesh sieves, the granule not by 100 mesh sieves is for subsequent use, and form graininess, underproof granule reclaims.
(7) fill
Granule pack step 6 prepared, makes granule of the present invention.
Embodiment 5: be prepared into soft capsule, powder, pill by above-mentioned same method.
Embodiment 6: pharmacological experiment
This laboratory utilizes above three kinds of primary raw materials to be aided with certain adjuvant and prepares qualified product (by the preparation of above-described embodiment 1 method) and carried out pharmaceutical research.Embodiment 6 is shown in experimental data statistical result.
1 materials and methods
1.1 material
1.1.1 animal cleaning grade db/db male mice, strain BSK.Cg-Dock7m+ /+Leprdb/NJU homozygote is used for experiment, weight 50-60g, cleaning grade C57 male mice, strain BSK.Cg-Dock7m+ /+Leprdb/NJU wild type is used for contrast, there is provided by Nanjing University-Nanjing biological medicine academy, credit number is SCXK (Soviet Union) 2010-0001.
1.1.2 the tablet that medicine Example 1 is obtained is as being subject to reagent A, and racemization thioctic acid is as positive drug.
1.1.3 reagent and the superior blood sugar test paper of instrument, blood glucose meter, purchased from American Roche Diagnistics company; Glutamate pyruvate transaminase, glutamic oxaloacetic transaminase, GOT, Bun (blood urea nitrogen), Crea (creatinine), blood fat [T-CHOL (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein, LDL (LDL)] test kit, build up Bioengineering Research Institute purchased from Nanjing; The long microplate reader of SpectraMax190 all-wave, MD company of the U.S.; ALLEGRAX-30Rcentrifuge refrigerated centrifuger, Beckman company of the U.S.; Electric heating constant-temperature water-bath tank, HHS type Shanghai Bo Xun industrial corporation; FA1104 electronic balance, Shanghai Sunny Hengping Scientific Instrument Co., Ltd.; AUW220D analytical balance, Japanese Shimadzu.
1.2 method
1.2.1 db/db and the C57 mice that animal divides into groups 8 week age enters experiment, is divided into five groups at random, often organizes 6: A group, C57 mice normal group; B group, db/db mouse model group; C group, positive drug group; D group, by reagent A small dose group; E group, by dosage group in reagent A; F group, by reagent A high dose group.Between morning day, the set time is with little, middle and high dosage gavage, and normal group gives the normal saline of identical high dose, and positive drug group gives the racemization thioctic acid of identical high dose.Successive administration 4 weeks.Experimental session, mice is raised in germfree animal room, and all utensils and food are all sterilized, sterile working.Mice ad lib, water inlet, keep bedding and padding dry, 12h is illumination alternately.
1.2.2 medication day early between the set time with low dose of (0.15g/kg), middle dosage (0.3g/kg), high dose (0.9g/kg) gavage, normal group gives the normal saline of identical high dose, positive drug group gives the racemization thioctic acid of identical high dose, successive administration 4 weeks.Experimental session, mice is raised in germfree animal room, and all utensils and food are all sterilized, sterile working.Mice ad lib, water inlet, keep bedding and padding dry, 12h is illumination alternately.
1.2.3 specimen collection experiment claims Mice Body quality (BW) before terminating.Mice to be placed in metabolic cage fasting 8 hours, get blood from mice vena orbitalis posterior clump under 10% urethane intraperitoneal anesthesia, separation of serum is to be measured in-20 DEG C of Refrigerator stores; Cut open the belly, get rapidly liver, kidney, claim liver mass (RW), kidney quality (SW), normal saline repeatedly rinses rear immersion 4% neutral formalin solution and fixes.
1.2.4 liver index calculates liver index (RI)=liver mass (RW)/Mice Body quality (BW) × 100.
1.2.5 renal index calculates renal index (SI)=kidney quality (SW)/Mice Body quality (BW) × 100.
1.2.6 liver functional testing glutamate pyruvate transaminase (ALT), glutamic oxaloacetic transaminase, GOT (AST) concrete operations are carried out according to test kit description and the long microplate reader mensuration of SpectraMax190 all-wave.
1.2.7 kidney index determining Bun (blood urea nitrogen), Crea (creatinine) concrete operations are carried out according to test kit description and the long microplate reader mensuration of SpectraMax190 all-wave.
1.2.8 blood lipids index measures T-CHOL (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein, LDL (LDL) concrete operations carry out according to test kit description and the long microplate reader of SpectraMax190 all-wave measures.
1.2.9 the above-mentioned renal tissue of diabetic mice Renal Paphology is after 4% neutral formalin solution is fixing, and routine paraffin wax embeds, slice thick 4 ~ 5 μm, changes after conventional H E dyeing in optical microphotograph Microscopic observation renal tissues pathology.
1.3 statistical analysis
Adopt SPSS17.0 statistical software to carry out variance analysis, measurement data represents with (± s), and compare between group with one factor analysis of variance, with P<0.05, result all represents that difference has statistical significance.
2. result
2.1 diabetes db/db mouse liver coefficients
By reagent A administration after 4 weeks, recording Normal group mouse liver index normal value is 0.0371 ± 0.003g, type 2 diabetes mellitus db/db mouse liver index is 0.051 ± 0.006g, significant differences (P<0.001) is had respectively with normal value, the enlargement of db/db mouse liver is described, liver lipids accumulates.By reagent A high dose group, middle dosage group, low dose group and model group liver index, only have trend, visible, do not have prejudicial effect trend by the liver of reagent A to db/db mice.The results are shown in Table 1.
Table 1. is subject to reagent A to the index variation of db/db mouse liver
Compared with normal group, * P<0.05, * * P<0.01, * * * P<0.001
2.2 diabetes db/db mouse kidney coefficients
Administration is after 4 weeks, recording Normal group mouse kidney index normal group is 0.013 ± 0.0008g, type 2 diabetes mellitus db/db mouse kidney index is 0.0097 ± 0.0009g, there is significant differences (P<0.001) with normal value respectively, illustrate that db/db mice exists kidney damage.By reagent A high dose group, middle dosage group, low dose group and model group liver index, renal index there was no significant difference, only have trend.Visible, do not have prejudicial effect trend by the kidney of reagent A to db/db mice.The results are shown in Table 2.
Table 2. is subject to reagent A to the index variation of db/db mouse kidney
Compared with normal group, * P<0.05, * * P<0.01, * * * P<0.001
2.3 are subject to reagent A on the impact of db/db mice serum AST, ALT
Observe impact db/db mouse liver damaged by reagent A.To be subject to reagent A gavage 4 weeks, observe db/db mice serum AST, ALT changes of contents.
By reagent A administration after 4 weeks, recording Normal group mice serum AST normal value is 24.890 ± 10.904mmolL-1, and type 2 diabetes mellitus db/db mice serum AST value is 28.297 ± 25.395mmolL-1, with normal group there was no significant difference; Recording Normal group mice serum ALT normal value is 12.191 ± 5.266mmolL-1, type 2 diabetes mellitus db/db mice serum ALT value is 60.934 ± 22.460mmolL-1, significant differences (P<0.01) is had with normal value, illustrate that infringement appears in db/db mouse liver, high by reagent A, in, only have trend without significant differences between low dose group and model group AST, ALT value group.The results are shown in Table 3.
Table 3. is subject to reagent A on the impact of db/db mice serum serum AST, ALT
Compared with normal group, * P<0.05, * * P<0.01, * * * P<0.001
The analysis of 2.4 blood serum Scr, Crea
Administration is after 4 weeks, recording Normal group mice serum Bun normal value is 6.703 ± 1.992mmolL-1, type 2 diabetes mellitus db/db mice serum Bun value is 11.018 ± 3.949mmolL-1, has significant difference (P<0.05) with normal group; Recording Normal group mice serum Crea normal value is 58.373 ± 25.774mmolL-1, type 2 diabetes mellitus db/db mice serum Crea value is 130.755 ± 60.790mmolL-1, significant differences (P<0.05) is had with normal value, illustrate that infringement appears in db/db mouse liver, high by reagent A, in, only have trend without significant differences between low dose group and model group Bun, Crea value group.The results are shown in Table 4.
Table 4. is subject to reagent A to the effect of db/db mouse kidney Bun, Crea
Compared with normal group, * P<0.05, * * P<0.01, * * * P<0.001
2.5 are subject to reagent A to db/db mice effect for reducing blood fat
Observe by reagent A the impact of db/db induced Hyperlipidemia in Mice.To be subject to reagent A gavage 4 weeks, observe db/db mice serum TC, TG, HDL, LDL changes of contents.Result of study shows: compare with model group, significantly reduce by the high, medium and low dosage group TG of reagent A, TC; Significantly reduce by reagent A high dose group LDL, HDL significantly raises.Result of study is pointed out, and has and reduces serum TG, TC, LDL, raise the effect of HDL by reagent A.
Recording Normal group mice serum TG normal value is 0.94 ± 0.18mmolL-1, type 2 diabetes mellitus db/db mice serum TG value is 1.20 ± 0.26mmolL-1, significant differences (P<0.01) is had with normal value, significant differences (P<0.001) is had by between reagent A high dose group and model group TG value group, between the TG value group of middle dosage group and model group, there were significant differences (P<0.01), the TG of small dose group and model group has significant difference (P<0.01), visible have the trend obviously reducing db/db mice serum triglyceride level by reagent A.The results are shown in Table 5.
Recording Normal group mice serum TC normal value is 1.076 ± 0.038mmolL-1, type 2 diabetes mellitus db/db mice serum TC value is 2.887 ± 0.574mmolL-1, significant differences (P<0.001) is had with normal value, significant differences (P<0.001) is had by between reagent A high dose group and model group TC value group, between the TC value group of middle dosage group and model group, there were significant differences (P<0.001), the TC of small dose group and model group has significant difference (P<0.01), visible have the trend obviously reducing db/db mice cholesterol levels by reagent A.The results are shown in Table 5.
Recording Normal group mice serum HDL-C normal value is 4.565 ± 1.507mmolL-1, type 2 diabetes mellitus db/db mice serum HDL-C value is 3.017 ± 0.267mmolL-1, significant differences (P<0.01) is had with normal value, significant differences (P<0.01) is had by between reagent A high dose group and model group HDL-C value group, the HDL-C value of middle dosage group and model group, the equal zero difference of HDL-C of small dose group and model group, only has trend.Visible have the trend raising db/db mice hdl level by reagent A.The results are shown in Table 5.
Recording Normal group mice serum LDL-C normal value is 0.140 ± 0.208mmolL-1, type 2 diabetes mellitus db/db mice serum LDL-C value is 1.271 ± 0.189mmolL-1, significant differences (P<0.01) is had with normal value, significant differences (P<0.001) is had by between reagent A high dose group and model group LDL-C value group, the LDL-C value of middle dosage group and model group, the LDL-C of small dose group and model group there are no significant difference, only variant, visible compound recipe has the trend reducing db/db mice low-density lipoprotein white level by reagent A.The results are shown in Table 5.
Table 5. is subject to reagent A on the impact of db/db mice serum TC, TG, HDL-C, LDL-C
Note: compared with normal group, * P<0.05, * * P<0.01, * * * P<0.001; Compared with model group,
#p<0.05,
##p<0.01,
###p<0.001
2.6 are subject to reagent A to db/db mouse kidney pathological change
Common HE dyeing display, under low power lens (10 × 10), the visible renal cells of diabetes db/db mouse kidney is slight granular degeneration, the atrophy of kidney visible renal tubules severe degeneration necrosis, renal cast; And the visible a large amount of cell infiltration of interstitial, glomerular mesangium, basement membrane obviously thicken.And after intervening by reagent A, the visible renal cells of kidney is slight granular degeneration, glomerular capillary is open good.Extracellular matrix mild hyperplasia, only a small amount of inflammatory cell infiltration, renal tubules structure is normal.Prompting the present invention has protection renal function.
Analyze above-mentioned Experiment Result known, this product significantly can reduce db/db diabetic mice serum TG, TC, LDL-C level, points out this product to have Metabolism regulation effect.Therefore my product of unit development has significant lipid metabolism regulating action, can also effectively protect liver and kidney simultaneously.Therefore the immunity degradation that causes from diabetes of this compatibility and glycolipid metabolism imbalance are as point of penetration, utilize the enhancing of leaf of Cyclocarya paliurus Iljinskaja, Folium Ginkgo, Cordyceps militaris (L.) Link. immunity and Metabolism regulation effect to carry out health product research and development and have certain scientific theory foundation.
Above-described embodiment is to explanation of the present invention, is not limitation of the invention, under the premise without departing from the principles of the invention, can also make some improvement and modification, and these improve and modify also should in protection scope of the present invention.Any all protection scope of the present invention is belonged to the scheme after simple transformation of the present invention.
Claims (5)
1. regulate a natural drug composition for diabetes immunity metabolic function, it is characterized in that, be made up of the raw material of following weight proportion: Cordyceps militaris (L.) Link., leaf of Cyclocarya paliurus Iljinskaja, Folium Ginkgo extract.
2. the natural drug composition of adjustment diabetes immunity metabolic function according to claim 1, is characterized in that, be made up of the raw material of following weight proportion: Cordyceps militaris (L.) Link. 40-55 part, leaf of Cyclocarya paliurus Iljinskaja 35-55 part, Folium Ginkgo extract 4-8 part.
3. the natural drug composition of adjustment diabetes immunity metabolic function according to claim 1, is characterized in that, be made up of the raw material of following weight proportion: Cordyceps militaris (L.) Link. 50 parts, leaf of Cyclocarya paliurus Iljinskaja 40 parts, Folium Ginkgo extract 6 parts.
4. the natural drug composition of adjustment diabetes according to claim 1 immunity metabolic function, is characterized in that, dosage form is any one in the other forms of dosage forms such as tablet in oral formulations, hard capsule, soft capsule, granule, powder, pill.
5. the natural drug composition of adjustment diabetes immunity metabolic function according to claim 1, it is characterized in that, preparation method comprises the following steps: (1) leaf of Cyclocarya paliurus Iljinskaja process: leaf of Cyclocarya paliurus Iljinskaja is clean, dry, add the water of 15-30 times amount, decoct after soaking 30-60min, adjust the temperature to micro-boiling after boiling, after keeping slight boiling condition 60min, collect filtrate; The remaining residue of institute take water as solvent, and the water adding 10-15 times amount continues to decoct, and adjusts the temperature to slight boiling condition after boiling, collects filtrate, repeat this step twice after keeping slight boiling condition 60min; Merge three filtrates, use heating in water bath when carrying out being concentrated into 60 DEG C the relative density of thick paste be 1.15-1.20, after carry out drying under reduced pressure, condition is 60 DEG C, and-0.06MPa, obtains dry extract; Dry gained dry extract is put into pulverizer to pulverize, cross 100 mesh sieves and obtain leaf of Cyclocarya paliurus Iljinskaja extract, discharging, for subsequent use; (2) Cordyceps militaris (L.) Link. process: Cordyceps militaris (L.) Link. is put into pulverizer and pulverize, cross 100 mesh sieves, discharging, for subsequent use; (3) Folium Ginkgo extract; (4) batch mixing: by the dry powder for subsequent use of (1), (2), (3) gained according to recipe requirements accurate weighing, put into mixer, mix homogeneously, obtain pulverulent mixture for subsequent use, makes various dosage form.
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| CN106261436A (en) * | 2016-08-16 | 2017-01-04 | 武汉市乐百龄生物科技有限公司 | A kind of food for treating diabetes |
| CN106963807A (en) * | 2017-04-21 | 2017-07-21 | 皖南医学院 | A kind of hypoglycemic oral liquid and preparation method thereof |
| CN107647408A (en) * | 2017-11-15 | 2018-02-02 | 山西素高生物工程有限公司 | A kind of functional food of auxiliary treatment CKD and preparation method thereof |
| CN112401239A (en) * | 2020-12-07 | 2021-02-26 | 广州正广生物科技有限公司 | Composition for reducing blood sugar and preparation method and application thereof |
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| CN106261436A (en) * | 2016-08-16 | 2017-01-04 | 武汉市乐百龄生物科技有限公司 | A kind of food for treating diabetes |
| CN106963807A (en) * | 2017-04-21 | 2017-07-21 | 皖南医学院 | A kind of hypoglycemic oral liquid and preparation method thereof |
| CN106963807B (en) * | 2017-04-21 | 2018-01-30 | 皖南医学院 | A kind of hypoglycemic oral liquid and preparation method thereof |
| CN107647408A (en) * | 2017-11-15 | 2018-02-02 | 山西素高生物工程有限公司 | A kind of functional food of auxiliary treatment CKD and preparation method thereof |
| CN112401239A (en) * | 2020-12-07 | 2021-02-26 | 广州正广生物科技有限公司 | Composition for reducing blood sugar and preparation method and application thereof |
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Application publication date: 20151223 |