CN105167175A - Enrichment method of peppery component suitable for preparing electronic cigarette liquid - Google Patents
Enrichment method of peppery component suitable for preparing electronic cigarette liquid Download PDFInfo
- Publication number
- CN105167175A CN105167175A CN201510741819.8A CN201510741819A CN105167175A CN 105167175 A CN105167175 A CN 105167175A CN 201510741819 A CN201510741819 A CN 201510741819A CN 105167175 A CN105167175 A CN 105167175A
- Authority
- CN
- China
- Prior art keywords
- tobacco
- enrichment method
- peppery
- enrichment
- electronic smoke
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Manufacture Of Tobacco Products (AREA)
Abstract
The invention relates to an enrichment method of a peppery component suitable for preparing an electronic cigarette liquid. The enrichment method is characterized by comprising the following steps: (1) extracting a tobacco leaf extract from tobacco leaves by virtue of a solvent; (2) weighing the tobacco leaf extract, carrying out centrifugal filtration and column chromatography separation, collecting each fraction according to the volume of eluent, and respectively marking the fractions as N1, N2,......, etc.; (3) after the fractions are tasted by at least three professional evaluation persons and the peppery fraction is determined, freeze-drying the peppery fraction by virtue of a freeze-drying machine, and enriching the peppery fraction; and (4) adding the enriched peppery fraction into propylene glycol or glycerinum, so as to prepare the electronic cigarette liquid. The enrichment method is guided through sensory evaluation, the tobacco leaf extract is separated by virtue of column chromatography, different numbers of the fractions are collected and are tasted, and the peppery fraction is freeze-dried by virtue of the freeze-drying machine and is enriched. The enrichment method has the advantages that the flow is simple, the operation is convenient, and the application range is wide; and furthermore, the obtained peppery component is derived from the tobacco leaves, so that the prepared electronic cigarette liquid has strong tobacco fragrance and can meet sensory requirements of smokers.
Description
Technical field
The invention belongs to tobacco product separation technology field, specifically a kind of enrichment method being applicable to the pungent component preparing tobacco juice for electronic smoke.
Background technology
Sense organ group (sensomics) is the concept (Angew.Chem.Int.Ed., 2011,50,2220-2242) that isolation technics and sensory evaluation technique combine and propose in order to the characteristic component finding the initiation food sense of taste by researcher.At present, what the technique study of application sense organ group was maximum is cause in natural products or the olfactive components of adjustment fragrance and sweet taste, delicate flavour, bitter taste, tart flavour taste component, in food analysis, have more application, but the application of the method in tobacco leaf analysis is not also reported.For tobacco leaf, pungent is very important for the impact of aesthetic quality.At present, carried out large quantity research both at home and abroad to fragrance component in tobacco leaf, define very ripe fragrance component separate evaluation method, but in tobacco leaf, the enrichment method of pungent component has not also been reported, relevant application study is not also carried out.
Electronic cigarette utilizes tobacco juice for electronic smoke to be heated the fog that can produce as cigarette by atomizer for electric, be mainly electronic cigarette liquid with the mixture of tobacco essence special and glycerine or propane diols, but utilize the electronic cigarette of electronic cigarette liquid and tradition to burn and suck cigarette at present to differ greatly to the sensory evaluation of consumer, always by consumer is discontented with.
Summary of the invention
A kind of enrichment method being applicable to the pungent component preparing tobacco juice for electronic smoke that object of the present invention provides based on above-mentioned prior art situation just, can be used for the development of Novel electric cigarette tobacco juice for the pungent component be enriched to.
The enrichment principle of foundation of the present invention is: the principle adopting sense organ group, is separated with Column chromatography techniques, is instruct, carries out enrichment to the pungent component existed in tobacco leaf with sensory evaluation.
The object of the invention is to be achieved through the following technical solutions:
Be applicable to an enrichment method for the pungent component preparing tobacco juice for electronic smoke, concrete steps are as follows:
(1) the tobacco leaf solvent extraction after processing is obtained leaf tobacco extract;
(2) take a certain amount of leaf tobacco extract, after filtration, by pillar layer separation, collect each flow point by effluent volume, be labeled as N1, N2, N3, N4, N5, N6, N7, N8, N9, N10 respectively ... Deng;
(3) sub-elect from each flow point there is pungent flow point after use freeze dryer freeze-drying, for the enrichment of pungent component;
(4) the pungent component be enriched to is added propane diols or glycerine, can tobacco juice for electronic smoke be made into.
In step of the present invention (3): get a share flow point, taste, the flow point containing pungent component is carried out merging, enrichment.Concrete assorting room judges personnel's trial test through the specialty of more than three, merged by the pungent flow point unanimously assert, uses freeze dryer freeze-drying after enrichment.It is through the personnel trained and correct with reference to standard GB/T/T12312-2012 and GB/T21265-2007 that this specialty judges personnel.
Described tobacco leaf can be upper tobacco leaf, middle part tobacco leaf, also can be lower tobacco leaf.
Described Extraction solvent can be any one
in water, ethanol, ethyl acetate, ether.
Described tobacco leaf processing extraction comprises normal heating method, supercharging heating, oil bath heating etc., temperature 25-100
oc, heat time 2-24 hour.
Described column chromatography is gel column chromatography.Eluant, eluent is distilled water.
The leaf tobacco extract enrichment method that the present invention relates to can be used for the pungent component in discovery, separation, enrichment tobacco leaf, can be used for the development of novel electron tobacco juice, solves electronic cigarette and traditional problem burning and sucking cigarette and differ greatly to the sensory evaluation of consumer.The present invention adopts the principle of sense organ group, utilizes Column chromatography techniques to be separated, and is to instruct with sensory evaluation, carries out enrichment to the pungent component existed in tobacco leaf.
The present invention has the following advantages: flow process is simple, easy to operate, applied widely, can be used for the development of novel electron tobacco juice, and prepared electronic cigarette liquid has more tobacco flavor, more can meet the organoleptic requirements of smoker.
Detailed description of the invention
The present invention in conjunction with the embodiments son is described further, but and limits the scope of the invention never in any form.
embodiment 1
The enrichment method of Tobacco in Henan C3F extract pungent component
After Henan flue cured tobacco C3F tobacco leaf being put into baking box oven dry, be ground into tobacco powder with pulverizer.Added in 1L round-bottomed flask by the 100g tobacco powder taken, add the ethanol of 600mL75%, backflow 4h, is chilled to room temperature, filters, and filtrate is concentrated on the Rotary Evaporators of pressure 50mbar, bath temperature 40 DEG C, obtains leaf tobacco extract.The Tobacco in Henan C3F extract taking 15.0 grams is dissolved in 30.0 ml distilled waters, filters.Then, loading is to gel chromatography, and applied sample amount 10 milliliters take distilled water as mobile phase, and every 20mL collects flow point, after tasting, find that N6, N7, N8, N13, N14 flow point contains pungent component.By the flow point merging containing pungent component, enrichment, use freeze dryer freeze-drying.The pungent component be enriched to and mixed solvent 1% are dissolved in propane diols and glycerine volume ratio is in the solvent of 1:1 in mass ratio, can tobacco juice for electronic smoke be made into.
embodiment 2
The enrichment method of Tobacco in Henan B2F extract pungent component
After Henan flue cured tobacco B2F tobacco leaf being put into baking box oven dry, be ground into tobacco powder with pulverizer.Added in 1L round-bottomed flask by the 100g tobacco powder taken, add 600mL absolute ethyl alcohol, backflow 4h, filters, and filtrate is concentrated on the Rotary Evaporators of pressure 50mbar, bath temperature 40 DEG C, obtains leaf tobacco extract.The Tobacco in Henan B2F extract taking 3.0 grams is dissolved in 6.0 ml distilled waters, filters.Then, loading is to gel chromatography, and applied sample amount 6 milliliters take distilled water as mobile phase, and every 20mL collects flow point, after tasting, find that N20, N22, N23, N24 flow point contains pungent component.By the flow point merging containing pungent component, enrichment, use freeze dryer freeze-drying.The pungent component be enriched to is got 2%, adds in the glycerine of 98% and can be made into tobacco juice for electronic smoke.
embodiment 3
The enrichment method of Tobacco in Henan X2F extract pungent component
After Henan flue cured tobacco X2F tobacco leaf being put into baking box oven dry, be ground into tobacco powder with pulverizer.Added in 1L round-bottomed flask by the 130g tobacco powder taken, add 700mL ethyl acetate, backflow 4h, filters, and filtrate is concentrated on the Rotary Evaporators of pressure 50mbar, bath temperature 40 DEG C, obtains leaf tobacco extract.The Tobacco in Henan X2F extract taking 18.9 grams is dissolved in 32.0 ml distilled waters, filters.Then, loading is to gel chromatography, and applied sample amount 9 milliliters take distilled water as mobile phase, and every 20mL collects flow point, find after tasting N12,13, N16, N17 flow point contains pungent component.By the flow point merging containing pungent component, enrichment, use freeze dryer freeze-drying.The pungent component be enriched to is got 1.5%, adds in the propane diols of 98.5% and can be made into tobacco juice for electronic smoke.
Claims (8)
1. be applicable to the pungent component enrichment method preparing tobacco juice for electronic smoke, it is characterized in that: the method concrete steps are as follows:
Tobacco leaf solvent extraction is obtained leaf tobacco extract;
Take a certain amount of leaf tobacco extract, after filtration, with pillar layer separation, collect each flow point by effluent volume, be labeled as N1, N2, N3, N4, N5, N6, N7, N8, N9, N10 respectively ... Deng;
Sub-elect from each flow point after there is pungent flow point and use freeze dryer freeze-drying, for the enrichment of pungent component;
The pungent component be enriched to is added propane diols or glycerine, can tobacco juice for electronic smoke be made into.
2. be according to claim 1ly applicable to the enrichment method of pungent component preparing tobacco juice for electronic smoke, it is characterized in that: described tobacco leaf is any one or its combination in upper tobacco leaf, middle part tobacco leaf, lower tobacco leaf.
3. the enrichment method being applicable to the pungent component preparing tobacco juice for electronic smoke according to claim 1, is characterized in that: described Extraction solvent can be any one in water, ethanol, ethyl acetate, ether.
4. the enrichment method being applicable to the pungent component preparing tobacco juice for electronic smoke according to claim 1, is characterized in that: described tobacco leaf extracts and comprises normal heating method, hyperbaric heating method, oil bath heating, temperature 25-100
oc, heat time 2-24 hour.
5. the enrichment method being applicable to the pungent component preparing tobacco juice for electronic smoke according to claim 1, is characterized in that: described eluent is distilled water.
6. the enrichment method being applicable to the pungent component preparing tobacco juice for electronic smoke according to claim 1, is characterized in that: described column chromatography is gel column chromatography.
7. the enrichment method being applicable to the pungent component preparing tobacco juice for electronic smoke according to claim 1, it is characterized in that: the assorting room of step (3) judges personnel's trial test through the specialty of more than three, the pungent flow point unanimously assert is merged, use freeze dryer freeze-drying after enrichment.
8. the enrichment method being applicable to the pungent component preparing tobacco juice for electronic smoke according to claim 7, is characterized in that: it is through the personnel trained and correct with reference to standard GB/T/T12312-2012 and GB/T21265-2007 that described specialty judges personnel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510741819.8A CN105167175B (en) | 2015-11-05 | 2015-11-05 | A kind of enrichment method being applicable to prepare the pungent component of tobacco juice for electronic smoke |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510741819.8A CN105167175B (en) | 2015-11-05 | 2015-11-05 | A kind of enrichment method being applicable to prepare the pungent component of tobacco juice for electronic smoke |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105167175A true CN105167175A (en) | 2015-12-23 |
| CN105167175B CN105167175B (en) | 2016-08-17 |
Family
ID=54890015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510741819.8A Expired - Fee Related CN105167175B (en) | 2015-11-05 | 2015-11-05 | A kind of enrichment method being applicable to prepare the pungent component of tobacco juice for electronic smoke |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105167175B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114680362A (en) * | 2021-12-28 | 2022-07-01 | 四川三联新材料有限公司 | Electronic cigarette liquid with refreshing function |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5381698A (en) * | 1976-12-28 | 1978-07-19 | Japan Tobacco Inc | Tobacco having improved taste |
| US4485828A (en) * | 1982-01-28 | 1984-12-04 | International Flavors & Fragrances Inc. | Use of substituted methyl isopropyl cyclohexenones in augmenting or enhancing the aroma or taste of smoking tobacco compositions and smoking tobacco article components |
| CN101209986A (en) * | 2006-12-29 | 2008-07-02 | 河南农业大学 | Microwave method for extracting beta-carotene from tobacco leaf |
| CN102206138A (en) * | 2010-12-30 | 2011-10-05 | 上海烟草集团有限责任公司 | Method for separating and purifying two fragrance precursors from tobacco |
| CN102759588A (en) * | 2012-07-16 | 2012-10-31 | 上海烟草集团有限责任公司 | Method for separating and preparing key acid flavor components in cigarette mainstream smoke and application of key acid volatile components |
| CN103134888A (en) * | 2013-01-21 | 2013-06-05 | 贵州省烟草科学研究院 | Analytic method of flue-cured tobacco volatile aroma components |
| CN103436363A (en) * | 2013-09-03 | 2013-12-11 | 华宝食用香精香料（上海）有限公司 | Preparation method of aroma substance in Zimbabwe tobacco extractive as well as products and application of aroma substance |
-
2015
- 2015-11-05 CN CN201510741819.8A patent/CN105167175B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5381698A (en) * | 1976-12-28 | 1978-07-19 | Japan Tobacco Inc | Tobacco having improved taste |
| US4485828A (en) * | 1982-01-28 | 1984-12-04 | International Flavors & Fragrances Inc. | Use of substituted methyl isopropyl cyclohexenones in augmenting or enhancing the aroma or taste of smoking tobacco compositions and smoking tobacco article components |
| CN101209986A (en) * | 2006-12-29 | 2008-07-02 | 河南农业大学 | Microwave method for extracting beta-carotene from tobacco leaf |
| CN102206138A (en) * | 2010-12-30 | 2011-10-05 | 上海烟草集团有限责任公司 | Method for separating and purifying two fragrance precursors from tobacco |
| CN102759588A (en) * | 2012-07-16 | 2012-10-31 | 上海烟草集团有限责任公司 | Method for separating and preparing key acid flavor components in cigarette mainstream smoke and application of key acid volatile components |
| CN103134888A (en) * | 2013-01-21 | 2013-06-05 | 贵州省烟草科学研究院 | Analytic method of flue-cured tobacco volatile aroma components |
| CN103436363A (en) * | 2013-09-03 | 2013-12-11 | 华宝食用香精香料（上海）有限公司 | Preparation method of aroma substance in Zimbabwe tobacco extractive as well as products and application of aroma substance |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114680362A (en) * | 2021-12-28 | 2022-07-01 | 四川三联新材料有限公司 | Electronic cigarette liquid with refreshing function |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105167175B (en) | 2016-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106387990B (en) | High-nicotine tobacco extract and its application | |
| CN106165906B (en) | Suitable for the burnt fragrant and sweet spices of the malt of cigarette products and the application tobacco product | |
| CN106174683B (en) | A kind of composition and the tobacco juice for electronic smoke containing said composition | |
| CN101586057B (en) | Essence for cigarette lap adhesive and preparation and use methods thereof | |
| CN103436363B (en) | Preparation method of aroma substance in Zimbabwe tobacco extractive as well as products and application of aroma substance | |
| CN104710542B (en) | The method for preparing polysaccharide and/or cigarette balm with flue-cured tobacco discarded tobacco leaf | |
| CN106509981A (en) | Preparation method of high-nicotine tobacco extract | |
| Demir et al. | Comparison of some extraction methods for isolation of catechins and caffeine from Turkish green tea | |
| CN103965369B (en) | Ganoderan, method for extraction and purification and the application as tobacco humectant thereof | |
| CN103783660B (en) | The supercritical extraction method of flavor matter and application thereof in a kind of tobacco leaf fragment | |
| CN105533793B (en) | A kind of preparation method of Dark sun-cured tobacco extract | |
| CN102586019B (en) | Preparation method and application of neutral aroma components in grain phase of smoke of high-quality tobacco leaves | |
| CN106307606A (en) | Method for preparing high-fragrance tobacco extract | |
| CN106318640A (en) | Preparation method of aromatic tobacco extract and application thereof in cigarettes | |
| CN106010797B (en) | A kind of burnt fragrant and sweet fragrance of the malt prepared using melanoidin malt as raw material and its application in electronic cigarette | |
| Kadam et al. | Evaluation of Ocimum sanctum and Ocimum basillicum mucilage-as a pharmaceutical excipient | |
| CN107912804A (en) | The method and its application of tobacco surface di-terpene and sugar esters compounds are extracted using tobacco crop field discarded object | |
| CN105831792A (en) | Method for preparing roasted fragrant and sweet components of red dates and application of roasted fragrant and sweet components of red dates to pipe tobacco | |
| CN108795574A (en) | A kind of preparation method for the structuring tobacco flavor particle being suitable for heating the cigarette that do not burn | |
| CN106167739A (en) | A kind of containing doing the microcapsule of extract of Folium Camelliae sinensis, Preparation Method And The Use | |
| CN103497834B (en) | Method for preparing dandelion absolute oil and application of dandelion absolute oil in cigarettes | |
| CN106174696A (en) | Prepare the method for Fructus Momordicae charantiae extract, Fructus Momordicae charantiae extract and application thereof | |
| CN103768367B (en) | A kind of expelling phlegm for arresting cough type tobacco oral spray agent and preparation method thereof | |
| CN105167175A (en) | Enrichment method of peppery component suitable for preparing electronic cigarette liquid | |
| CN105077552B (en) | Acanthopanax brachypus polysaccharide is as tobacco humectant application in Medicated cigarette |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160817 Termination date: 20181105 |