CN105166929B - Health drink beneficial to sleep as well as preparation method and detection method of health drink - Google Patents
Health drink beneficial to sleep as well as preparation method and detection method of health drink Download PDFInfo
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- CN105166929B CN105166929B CN201510643602.3A CN201510643602A CN105166929B CN 105166929 B CN105166929 B CN 105166929B CN 201510643602 A CN201510643602 A CN 201510643602A CN 105166929 B CN105166929 B CN 105166929B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 238000001514 detection method Methods 0.000 title claims abstract description 15
- 230000009286 beneficial effect Effects 0.000 title abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 62
- 239000000706 filtrate Substances 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229930006000 Sucrose Natural products 0.000 claims abstract description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 9
- 230000001954 sterilising effect Effects 0.000 claims abstract description 9
- 239000005720 sucrose Substances 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 120
- KZMACGJDUUWFCH-UHFFFAOYSA-O malvidin Chemical compound COC1=C(O)C(OC)=CC(C=2C(=CC=3C(O)=CC(O)=CC=3[O+]=2)O)=C1 KZMACGJDUUWFCH-UHFFFAOYSA-O 0.000 claims description 73
- 239000000243 solution Substances 0.000 claims description 57
- 235000009584 malvidin Nutrition 0.000 claims description 36
- 239000013558 reference substance Substances 0.000 claims description 34
- 238000012360 testing method Methods 0.000 claims description 33
- 241001106412 Pilea Species 0.000 claims description 24
- 241001093951 Ailanthus altissima Species 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 17
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 14
- 239000012141 concentrate Substances 0.000 claims description 11
- 238000004064 recycling Methods 0.000 claims description 11
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- 239000012467 final product Substances 0.000 claims description 8
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- 238000005303 weighing Methods 0.000 claims description 8
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- 239000011550 stock solution Substances 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 6
- 239000012982 microporous membrane Substances 0.000 claims description 6
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 5
- 238000012372 quality testing Methods 0.000 claims description 4
- 230000001737 promoting effect Effects 0.000 claims description 3
- 235000013361 beverage Nutrition 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 241001122767 Theaceae Species 0.000 claims 2
- 244000269722 Thea sinensis Species 0.000 abstract description 8
- 239000000203 mixture Substances 0.000 abstract description 4
- 235000011783 Cedrela sinensis Nutrition 0.000 abstract description 3
- 241001106411 Pilea pumila Species 0.000 abstract description 3
- 241000425037 Toona sinensis Species 0.000 abstract description 3
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- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 14
- 206010022437 insomnia Diseases 0.000 description 14
- 239000000523 sample Substances 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 239000002504 physiological saline solution Substances 0.000 description 8
- 239000013641 positive control Substances 0.000 description 8
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- 241000699670 Mus sp. Species 0.000 description 6
- 239000012071 phase Substances 0.000 description 6
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- 230000002618 waking effect Effects 0.000 description 2
- 244000271246 Cedrela sinensis Species 0.000 description 1
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010021703 Indifference Diseases 0.000 description 1
- 206010061245 Internal injury Diseases 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
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- 206010033557 Palpitations Diseases 0.000 description 1
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000725605 Pilea notata Species 0.000 description 1
- 241000040599 Prunus mongolica Species 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
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- 230000036461 convulsion Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- AAOVKJBEBIDNHE-UHFFFAOYSA-N diazepam Chemical compound N=1CC(=O)N(C)C2=CC=C(Cl)C=C2C=1C1=CC=CC=C1 AAOVKJBEBIDNHE-UHFFFAOYSA-N 0.000 description 1
- 229960003529 diazepam Drugs 0.000 description 1
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- 206010025482 malaise Diseases 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- MKELYWOVSPVORM-DTWKUNHWSA-N reposal Chemical compound C([C@@H]1CC[C@@H](C1)C=1)C=1C1(CC)C(=O)NC(=O)NC1=O MKELYWOVSPVORM-DTWKUNHWSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Physics & Mathematics (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of food processing and particularly relates to a health drink beneficial to sleep as well as a preparation method and a detection method of the health drink. The health drink is prepared from raw materials in parts by weight as follows: 1-5 parts of Pilea pumila and 1-5 parts of flowers of Toona sinensis. The preparation method of the health drink comprises steps as follows: the Pilea pumila and the Toona sinensis are taken as per the weight ratio in a prescription, 5-15 times of water is added for decoction for 1-2 h, 5-10 times of water is added to decoction dregs for decoction for 0.5-1 h, water decoctions are mixed and filtered, a filtrate is concentrated and cooled, ethyl alcohol with the volume concentration of 95% is added until the concentration of the ethyl alcohol ranges from 60% to 75%, the mixture is stirred, then left to stand at the temperature of 4-8 DEG C for 12-48 h and filtered, the ethyl alcohol is recovered from a filtrate, a health tea extract solution is obtained, sucrose and water are added, sterilization is performed, and the health drink is prepared.
Description
Technical field
The invention belongs to food processing technology field, it is related to a kind of health drink, specially one kind has promotion sleep work(
The health drink of energy.
Background technology
Insomnia, i.e. sleep disorder.Show as difficulty falling asleep, intermittently discontinuous, and prematurely wake up, can not be again after waking up
Continue to sleep, do not have enough sleep, malaise, burnout is felt, how because health condition is not good, pain, does not feel like oneself, circadian rhythm quilt
Upset, sleep environment impact etc., also have fearness to sleep and have a sleepless night.
Insomnia is due to feelings will, diet internal injury, or after being ill and old, insufficiency of natural endowment, the cause of disease such as have a guilty consicence, and causes the mind
Lose and support or confused and worried, thus leading to often to obtain the class illness that ortho is characterized.When being mainly shown as sleep
Between the deficiency of depth and be unable to dispelling fatigue, regain one's strength and energy, the lighter's difficulty falling asleep, or sleep but not soundly, when when sleeping
Wake up, or can not sleep again after waking up, heavy then be insomnia all night.
Insomnia is one of common clinical disease, though being not belonging to critical illness, hinders people's normal life, work, study
And health, and can increase or induce the illnesss such as palpitaition, the obstruction of qi in the chest, dizziness, headache, apoplexy.Obstinate insomnia, brings to patient
Long-term misery, or even form the dependence to sleeping medicine, and the sleeping medicine of long-term taking can cause iatrogenic disease.
Insomnia exists《Interior warp》In referred to as " insomnia ", " must not sleep ", " must not crouch ", and think that insomnia reason mainly has two
Kind：One is other illnesss impact, and such as cough, vomiting, abdomen is full etc., must not make one reposal；Two is that negative and positive of qi and blood is become estranged, and making one can not
Fall asleep.
In recent years, inventor adopts health drink of the present invention to be used for treatment insomnia, achieves preferable curative effect.
In the present invention：
Pilea mongolica is Urticaceae Pilea plant pilea mongolicaPilea pumila (L.) A. Cray [Urtica pumila L.; P. mongolica Wedd.]Herb or rhizome.
Ailanthus altissima flower is Meliaceae Chinese toon platymiscium Chinese toon Toona sinensis (A. Juss.) Roem. [Cedrela
Sinensis A. Juss.] flower.
Content of the invention
It is an object of the invention to provide a kind of have the health drink promoting sleep function.
It is a further object of the present invention to provide the preparation method of this health drink.
The purpose of the present invention is achieved in the following ways：
A kind of have the health drink promoting sleep function, is made up of the raw material of following weight parts：Pilea mongolica 1～
2 parts, 1～2 part of Ailanthus altissima flower.
Described health drink is preferably made up of the raw material of following weight parts：1 part of pilea mongolica, 1 part of Ailanthus altissima flower.
Described health drink is preferably made up of the raw material of following weight parts：1 part of pilea mongolica, 2 parts of Ailanthus altissima flower.
Described health drink is preferably made up of the raw material of following weight parts：2 parts of pilea mongolica, 1 part of Ailanthus altissima flower.
This health drink is made up of following methods：Take pilea mongolica, Ailanthus altissima flower by prescription weight proportion, plus 5～15 times
Decocting boils 1～2 hour, and the dregs of a decoction add 5 again～and 10 times amount decoctings boil 0.5～1 hour, and decocting liquid merges, and filters, and filtrate concentrates, cold
But, add the ethanol that volumetric concentration is 95% to be 60～75% to concentration of alcohol, be put in 4～8 DEG C after stirring and stand 12～48 hours,
Filter, filtrate recycling ethanol, obtain health care tea extract, with sucrose, add water, sterilizing, make health drink.
This health drink is preferably made up of following methods：Take pilea mongolica, Ailanthus altissima flower by prescription weight proportion, plus 10 times
Decocting boils 1.5 hours, and the dregs of a decoction add 6 times amount decoctings again and boil 1 hour, and decocting liquid merging twice is filtered, filtrate concentrates, cooling, added
Volumetric concentration be 95% ethanol to concentration of alcohol be 70%, be put in after stirring 5 DEG C stand 24 hours, filter, filtrate recycling ethanol,
Obtain health care tea extract, with sucrose, add water, sterilizing, make health drink.
This health drink carries out quality testing using following methods：
Using high effective liquid chromatography for measuring malvidin（malvidin）Content：
（1）Chromatographic condition：Chromatographic column is C18Chromatographic column；With ratio for 40～60：40～60 methyl alcohol -0.15% phosphoric acid is molten
Liquid is mobile phase；Detection wavelength 300～330nm, 25～45 DEG C of column temperature；
（2）The preparation of reference substance solution：Precision weighs malvidin reference substance and is placed in volumetric flask, plus methyl alcohol dissolves and dilute
Release to scale, shake up；Pipette above-mentioned solution and put volumetric flask, plus methanol constant volume, as reference substance stock solution；
（3）The preparation of need testing solution：Take health drink of the present invention, precision weighing, put in conical flask with cover, accurate addition
Methyl alcohol, close plug, weighed weight, ultrasonically treated, let cool, more weighed weight, supply the weight of less loss with methyl alcohol, shake up, micropore is filtered
Membrane filtration, takes subsequent filtrate, obtains final product need testing solution；
（4）Measure：Precision measures above-mentioned need testing solution, each 5～20 μ L of reference substance solution respectively, injects high-efficient liquid phase color
Spectrometer, is detected.
This health drink preferably employs following methods and carries out quality testing：Using high effective liquid chromatography for measuring malvidin
Content：
（1）Chromatographic condition：Chromatographic column is C18Chromatographic column；With ratio for 58：42 methyl alcohol -0.15% phosphoric acid solution is flowing
Phase；Detection wavelength 328nm, 32 DEG C of column temperature；Number of theoretical plate is calculated by malvidin peak and should be not less than 3000；
（2）The preparation of reference substance solution：Precision weighs malvidin reference substance 10.00mg and is placed in 100mL volumetric flask, plus
Methyl alcohol dissolves and is diluted to scale, shakes up；Pipette above-mentioned solution 10mL and put 25mL volumetric flask, plus methanol constant volume, as reference substance
Stock solution；
（3）The preparation of need testing solution：Take health drink 10mL of the present invention, precision weighing, put in conical flask with cover, accurate
Add methyl alcohol 10mL, close plug, weighed weight, ultrasonically treated 30min, let cool, more weighed weight, the weight of less loss is supplied with methyl alcohol
Amount, shakes up, 0.45 μm of filtering with microporous membrane, takes subsequent filtrate, obtain final product need testing solution；
（4）Measure：Precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, injects high performance liquid chromatography
Instrument, is detected.
Described health drink can be used for preparing the health drink for the treatment of insomnia.
Experiment one：The experimental study of health drink treatment insomnia of the present invention
1 experiment material
Health drink of the present invention：Prescription：Pilea mongolica 5kg, Ailanthus altissima flower 5kg.Preparation method：Take stem by prescription weight proportion
Pilea notata, Ailanthus altissima flower, plus 10 times of decoctings boil 1.5 hours, the dregs of a decoction add 6 times amount decoctings again and boil 1 hour, and decocting liquid merges twice, mistake
Filter, filtrate concentrates, cooling, adds the ethanol that volumetric concentration is 95% to be 70% to concentration of alcohol, is put in 5 DEG C of standings 24 little after stirring
When, filter, filtrate recycling ethanol, obtain health care tea extract of the present invention.
Contrast health drink A：Prescription：Pilea mongolica 10kg.Preparation method：Take pilea mongolica by prescription weight, plus 10 times
Decocting boils 1.5 hours, and the dregs of a decoction add 6 times amount decoctings again and boil 1 hour, and decocting liquid merging twice is filtered, filtrate concentrates, cooling, added
Volumetric concentration be 95% ethanol to concentration of alcohol be 70%, be put in after stirring 5 DEG C stand 24 hours, filter, filtrate recycling ethanol,
Obtain contrasting health drink A extract.
Contrast health drink B：Prescription：Ailanthus altissima flower 10kg.Preparation method：Take Ailanthus altissima flower by prescription weight proportion, plus 10 times of decoctings
Boil 1.5 hours, the dregs of a decoction add 6 times amount decoctings again and boil 1 hour, decocting liquid merging twice is filtered, filtrate concentrates, cooling, added volume
Concentration be 95% ethanol to concentration of alcohol be 70%, be put in after stirring 5 DEG C stand 24 hours, filter, filtrate recycling ethanol, obtain
Contrast health drink B extract.
Yellow Jackets：Beijing land for growing field crops Feng Tuo chemical technology Co., Ltd produces；
Strychnine nitrate parenteral solution：Beijing Double-Crane Pharmaceutical Co., Ltd produces；
Diazepam：Tianjin KingYork Amino Acid Co., Ltd. produces；
Animal：Kunming mouse, body weight 18g～22g, female dual-purpose, provided by Gansu university of TCM experimental center.
2 experimental techniques and result
The research of 2.1 pairs of spontaneous activity in mice
Mouse 60, male and female are regardless of, and are randomly divided into five groups, i.e. saline control group, contrast health drink A group, contrast
Health drink B group, health drink group of the present invention, stable positive controls, every group 12.
Before administration, mouse is put into after adapting to 5 minutes in animal activity case, record animal walking time and the forelimb of 2 minutes
The numerical value praising number of times upwards is as normal value.
Then give respectively：
Contrast health drink A group：Gavage gives contrast health drink A extract, and given low is 0.5mL/10g；
Contrast health drink B group：Gavage gives contrast health drink B extract, and given low is 0.5mL/10g；
Health drink group of the present invention：To health drink extract of the present invention, given low is 0.5mL/10g to gavage；
Stable positive controls give 0.01mg/10g and stabilize, gavage；
Saline control group gives same volume physiological saline, gavage.
Each group mouse, in latter 60 minutes of administration, measures mouse walking time by the same method and double forelimb praises number of times upwards.Knot
Fruit with± s represents, the results are shown in Table 1-1.
The impact to spontaneous activity in mice number of times for the table 1-1（± s, n=12）
Note：Compare with physiological saline group, * P<0.05, * * P< 0.01；
In table, result shows：Health drink of the present invention all has obvious sedation to mouse, compares with physiological saline group
There were significant differences（P< 0.01）.Health drink group sedation of the present invention substantially, compares indifference with stable group（P> 0.05）.
Result shows：Health drink of the present invention has obvious sedation.
2.2 researchs on mice sleep impact
2.2.1 the impact to the mouse yellow Jackets length of one's sleep
Mouse 60, is randomly divided into 5 groups, i.e. saline control group, contrast health drink A group, contrast health drink B
Group, health drink group of the present invention, stable positive controls, every group 12.
Contrast health drink A group：Gavage gives contrast health drink A extract, and given low is 0.5mL/10g；
Contrast health drink B group：Gavage gives contrast health drink B extract, and given low is 0.5mL/10g；
Health drink group of the present invention：To health drink extract of the present invention, given low is 0.5mL/10g to gavage；
Stable positive controls give 0.01mg/10g and stabilize, gavage；
Saline control group gives same volume physiological saline, gavage.
After gastric infusion 60 minutes, every group of mouse gives yellow Jackets 40mg/kg, lumbar injection respectively.Record mouse
The length of one's sleep（With righting reflex loss as time for falling asleep, from righting reflex loss to recovery time for sleep time）.
The results are shown in Table 1-2.
The impact to the mouse yellow Jackets length of one's sleep for the table 1-2（± s, n=12）
2.2.2 the impact to mouse yellow Jackets sub-threshold dose
Mouse 60, is randomly divided into 5 groups, i.e. saline control group, contrast health drink A group, contrast health drink B
Group, health drink group of the present invention, stable positive controls, every group 12.
Contrast health drink A group：Gavage gives contrast health drink A extract, and given low is 0.5mL/10g；
Contrast health drink B group：Gavage gives contrast health drink B extract, and given low is 0.5mL/10g；
Health drink group of the present invention：To health drink extract of the present invention, given low is 0.5mL/10g to gavage；
Stable positive controls give 0.01mg/10g and stabilize, gavage；
Saline control group gives same volume physiological saline, gavage.
After administration 30 minutes, each group mouse gives yellow Jackets 30mg/kg, lumbar injection respectively, observes and is recorded into
Sleep number of animals（In 15 minutes, mouse righting reflex loss reaches mouse number on 1 minute as sleep number of animals）, calculate sleep rate.Knot
Fruit is shown in Table 1-3.
The impact to mouse pentobarbital sodium sub-threshold lull dosage for the table 1-3
Above two experiments all illustrate that health drink of the present invention and yellow Jackets have synergy, and health care of the present invention is described
Beverage plays the role of hypnosis.
The research of 2.3 anti-strychnine nitrate convulsive attack effects
Mouse 60, male and female are regardless of, and are randomly divided into 5 groups, i.e. saline control group, contrast health drink A group, contrast
Health drink B group, health drink group of the present invention, stable positive controls, every group 12.
Contrast health drink A group：Gavage gives contrast health drink A extract, and given low is 0.5mL/10g；
Contrast health drink B group：Gavage gives contrast health drink B extract, and given low is 0.5mL/10g；
Health drink group of the present invention：To health drink extract of the present invention, given low is 0.5mL/10g to gavage；
Stable positive controls give 0.01mg/10g and stabilize, gavage；
Saline control group gives same volume physiological saline, gavage.
Each group mouse, after administration 60 minutes, gives strychnine nitrate parenteral solution 1.5mg/kg, hypodermic injection, note respectively
Record mice convulsion number（With mouse, generalized tonic occurs to faint from fear as index）.The results are shown in Table 1-4.
The effect of table 1-4 anti-strychnine nitrate convulsive attack（n= 12）
Note：Compare with physiological saline group：**P < 0.01；
Result shows, stable group and health drink group of the present invention all play the role of to resist strychnine nitrate convulsive attack, with
Physiological saline group compares, and is statistically analyzed the equal significance of difference.The anticonvulsant action of health drink of the present invention is strong, curative effect
Good, illustrate that health drink of the present invention has anticonvulsant effect.
3 conclusions
The study find that, health drink of the present invention can suppress spontaneous activity in mice；Impact to mice sleep and penta bar ratio
Appropriate sodium has synergy；Play the role of to resist strychnine nitrate convulsive attack.Illustrate health drink of the present invention have obvious calm,
Hypnosis, anticonvulsant action, have the prospect of exploitation medicament for treating insomnia or health products.
Particularly, the action effect of health drink of the present invention is substantially better than contrast health drink A and contrast health drink B,
Illustrate that in health drink of the present invention, compatibility between two kinds of flavour of a drug is superior, indispensable, the combination between two kinds of flavour of a drug creates
Significantly synergistic function, creates the technique effect that one-plus-one is more than two.
Experiment two：The quality standard research of health drink of the present invention
Using malvidin content in high effective liquid chromatography for measuring health drink of the present invention
The preparation of 1 sample solution
The preparation of 1.1 reference substance solution
Precision weighs malvidin reference substance 10.00mg and is placed in 100mL volumetric flask, plus methyl alcohol dissolves and is diluted to quarter
Degree, shakes up.Pipette above-mentioned solution 10mL and put 25mL volumetric flask, plus methanol constant volume, as reference substance stock solution（Malvidin
40.80μg/mL）.
The preparation of 1.2 need testing solutions
Prescription：Pilea mongolica 5kg, Ailanthus altissima flower 5kg.
Preparation method：Take pilea mongolica, Ailanthus altissima flower by prescription weight proportion, plus 10 times of decoctings boil 1.5 hours, the dregs of a decoction add 6 again
Times amount decocting boils 1 hour, and decocting liquid merging twice is filtered, filtrate concentrates, cooling, adds the ethanol that volumetric concentration is 95% to second
Determining alcohol is 70%, is put in 5 DEG C and stands 24 hours, filter, filtrate recycling ethanol, obtain health care tea extract after stirring, plus in right amount
Sucrose, add water, sterilizing, make health drink 20L.
Take health drink 10mL of the present invention, precision weighing, put in conical flask with cover, accurate addition methyl alcohol 10mL, close plug, claim
Determine weight, ultrasonically treated 30min, let cool, more weighed weight, supply the weight of less loss with methyl alcohol, shake up, 0.45 μm of miillpore filter
Filter, take subsequent filtrate, obtain final product need testing solution.
The preparation of 1.3 negative controls
Remaining composition taking in prescription composition in addition to pilea mongolica makes the negative controls without pilea mongolica, presses
" 1.2 " are obtained negative control solution.Go out peak position in malvidin and absworption peak do not occur, illustrate in health drink of the present invention its
He does not interfere with to the assay of malvidin composition.
2 chromatographic conditions
Chromatographic column is SHIMADZU C18Chromatographic column（5 μm, 4.6mm × 150.0mm, Shimadzu Corporation）；With methyl alcohol -0.15% phosphorus
Acid solution（58：42）For mobile phase；Detection wavelength 328nm, 32 DEG C of column temperature.Number of theoretical plate is calculated and should be not less than by malvidin peak
3000；With this understanding, in health drink of the present invention, malvidin separates well.
3 methodological studies.
3.1 the range of linearity
Accurate draw above-mentioned malvidin reference substance solution, plus methanol dilution become malvidin concentration to be respectively 0.816,
1.632nd, the solution of 3.264,4.896,6.528,8.160 μ g/mL, sample introduction 10 μ l successively.With peak area（Y）For ordinate, with
Sample introduction concentration（X）Carry out linear regression for abscissa, obtain regression equation：
Y=3.638 × 104X 1.068 × 104, correlation coefficient r=0.9996.Show malvidin concentration 0.816～
With chromatographic peak area in good linear relation in the range of 8.160 μ g/mL.
3.2 precision test
Take the malvidin reference substance solution 10 μ l METHOD FOR CONTINUOUS DETERMINATION that concentration is 4.890 μ g/mL 5 times, RSD is 0.15%, shows
Instrument precision is good.
3.3 stability test
Take same need testing solution 0,2,4,6,8h sample introduction 10 μ l respectively, record peak area, result shows：Test sample is molten
Liquid is placed in 8h in room temperature and is stablized, and malvidin peak area RSD is 1.60%.
3.4 replica test
By health drink of the present invention, parallel extraction prepares 6 parts of need testing solutions, and sample introduction measures respectively, and RSD is 1.23%（n=
6）, show that the method repeatability is good.
3.5 average recovery tests
Precision weighs each 9 parts of the health drink of the present invention of known content, adds the malvidin reference substance of respective amount, by confession
The preparation method of test sample solution processes and measures.Average recovery result of the test is shown in Table 2-1.Malvidin average recovery rate is
97.7%, RSD are 1.66%, show that the method is accurate, reliable.
Table 2-1 recovery test result（n=9）
3.6 sample sizes measure.Take 4 batches, health drink sample of the present invention, according to the method for need testing solution preparation, 0.45 μ
Sample introduction after m filtering with microporous membrane, different lot number health drink content of the present invention is shown in Table 2-2.
Table 2-2 sample determination result（n=3）
Experimental example three：Model case
Lee tries to gain, man, 61 years old, retired worker, because cerebrovascular disease goes into hospital before 3 months, because stress is big, causes mistake
Sleep, after discharge, insomnia is not healed, and through multi-treatment, effect is bad, have palpitation, shortness of breath, palpitaition, dizziness, check and find that tongue is red, the heart
Rule is uneven, One's spirits are drooping depressed, confirms as slightly having a sleepless night.
Drink health drink of the present invention, drink 1000mL daily, fraction is drunk, and continuously drinks 7 days, insomnia is cured, and has no
Any bad reaction.
Specific embodiment：
Embodiment 1：
Prescription：Pilea mongolica 5kg, Ailanthus altissima flower 5kg.
Preparation method：Take pilea mongolica, Ailanthus altissima flower by prescription weight proportion, plus 10 times of decoctings boil 1.5 hours, the dregs of a decoction add 6 again
Times amount decocting boils 1 hour, and decocting liquid merging twice is filtered, filtrate concentrates, cooling, adds the ethanol that volumetric concentration is 95% to second
Determining alcohol is 70%, is put in 5 DEG C and stands 24 hours, filter, filtrate recycling ethanol, obtain health care tea extract after stirring, plus in right amount
Sucrose, add water, sterilizing, make health drink 20L.
Detection method：Using high effective liquid chromatography for measuring malvidin content：
（1）Chromatographic condition：Chromatographic column is SHIMADZU C18Chromatographic column；With ratio for 58：42 methyl alcohol -0.15% phosphoric acid is molten
Liquid is mobile phase；Detection wavelength 328nm, 32 DEG C of column temperature；Number of theoretical plate is calculated by malvidin peak and should be not less than 3000；
（2）The preparation of reference substance solution：Precision weighs malvidin reference substance 10.00mg and is placed in 100mL volumetric flask, plus
Methyl alcohol dissolves and is diluted to scale, shakes up；Pipette above-mentioned solution 10mL and put 25mL volumetric flask, plus methanol constant volume, as reference substance
Stock solution；
（3）The preparation of need testing solution：Take health drink 10mL of the present invention, precision weighing, put in conical flask with cover, accurate
Add methyl alcohol 10mL, close plug, weighed weight, ultrasonically treated 30min, let cool, more weighed weight, the weight of less loss is supplied with methyl alcohol
Amount, shakes up, 0.45 μm of filtering with microporous membrane, takes subsequent filtrate, obtain final product need testing solution；
（4）Measure：Precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, injects high performance liquid chromatography
Instrument, is detected；
（5）Result：In every 10mL health drink of the present invention, malvidin content is 55.25 μ g.
Embodiment 2：
Prescription：Pilea mongolica 6.6kg, Ailanthus altissima flower 3.3kg.
Preparation method：Take pilea mongolica, Ailanthus altissima flower by prescription weight proportion, plus 10 times of decoctings boil 1.5 hours, the dregs of a decoction add 6 again
Times amount decocting boils 1 hour, and decocting liquid merging twice is filtered, filtrate concentrates, cooling, adds the ethanol that volumetric concentration is 95% to second
Determining alcohol is 70%, is put in 5 DEG C and stands 24 hours, filter, filtrate recycling ethanol, obtain health care tea extract after stirring, plus in right amount
Sucrose, add water, sterilizing, make health drink 20L.
Detection method：Using high effective liquid chromatography for measuring malvidin content：
（1）Chromatographic condition：Chromatographic column is SHIMADZU C18Chromatographic column；With ratio for 58：42 methyl alcohol -0.15% phosphoric acid is molten
Liquid is mobile phase；Detection wavelength 328nm, 32 DEG C of column temperature；Number of theoretical plate is calculated by malvidin peak and should be not less than 3000；
（2）The preparation of reference substance solution：Precision weighs malvidin reference substance 10.00mg and is placed in 100mL volumetric flask, plus
Methyl alcohol dissolves and is diluted to scale, shakes up；Pipette above-mentioned solution 10mL and put 25mL volumetric flask, plus methanol constant volume, as reference substance
Stock solution；
（3）The preparation of need testing solution：Take health drink 10mL of the present invention, precision weighing, put in conical flask with cover, accurate
Add methyl alcohol 10mL, close plug, weighed weight, ultrasonically treated 30min, let cool, more weighed weight, the weight of less loss is supplied with methyl alcohol
Amount, shakes up, 0.45 μm of filtering with microporous membrane, takes subsequent filtrate, obtain final product need testing solution；
（4）Measure：Precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, injects high performance liquid chromatography
Instrument, is detected；
（5）Result：In every 10mL health drink of the present invention, malvidin content is 33.54 μ g.
Embodiment 3：
Prescription：Pilea mongolica 3.3kg, Ailanthus altissima flower 6.6kg.
Preparation method：Take pilea mongolica, Ailanthus altissima flower by prescription weight proportion, plus 10 times of decoctings boil 1.5 hours, the dregs of a decoction add 6 again
Times amount decocting boils 1 hour, and decocting liquid merging twice is filtered, filtrate concentrates, cooling, adds the ethanol that volumetric concentration is 95% to second
Determining alcohol is 70%, is put in 5 DEG C and stands 24 hours, filter, filtrate recycling ethanol, obtain health care tea extract after stirring, plus in right amount
Sucrose, add water, sterilizing, make health drink 20L.
Detection method：Using high effective liquid chromatography for measuring malvidin content：
（1）Chromatographic condition：Chromatographic column is SHIMADZU C18Chromatographic column；With ratio for 58：42 methyl alcohol -0.15% phosphoric acid is molten
Liquid is mobile phase；Detection wavelength 328nm, 32 DEG C of column temperature；Number of theoretical plate is calculated by malvidin peak and should be not less than 3000；
（2）The preparation of reference substance solution：Precision weighs malvidin reference substance 10.00mg and is placed in 100mL volumetric flask, plus
Methyl alcohol dissolves and is diluted to scale, shakes up；Pipette above-mentioned solution 10mL and put 25mL volumetric flask, plus methanol constant volume, as reference substance
Stock solution；
（3）The preparation of need testing solution：Take health drink 10mL of the present invention, precision weighing, put in conical flask with cover, accurate
Add methyl alcohol 10mL, close plug, weighed weight, ultrasonically treated 30min, let cool, more weighed weight, the weight of less loss is supplied with methyl alcohol
Amount, shakes up, 0.45 μm of filtering with microporous membrane, takes subsequent filtrate, obtain final product need testing solution；
（4）Measure：Precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, injects high performance liquid chromatography
Instrument, is detected；
（5）Result：In every 10mL health drink of the present invention, malvidin content is 56.53 μ g.
Moreover, it will be appreciated that although this specification is been described by according to embodiment, not each embodiment only wraps
Containing an independent technical scheme, only for clarity, those skilled in the art should for this narrating mode of specification
Using specification as an entirety, the technical scheme in each embodiment can also form those skilled in the art through appropriately combined
Understandable other embodiment.
Claims (4)
1. a kind of have the health drink promoting sleep function, improvement to have a sleepless night it is characterised in that this health drink is by following heavy
The raw material of amount part is made：1 part of pilea mongolica, 1 part of Ailanthus altissima flower, this health drink is made up of following methods：Join by prescription weight
Ratio takes pilea mongolica, Ailanthus altissima flower, plus 5～15 times of decoctings boil 1～2 hour, and the dregs of a decoction add 5 again～and that 10 times amount decoctings boil 0.5～1 is little
When, decocting liquid merges, and filters, and filtrate concentrates, cooling, adds the ethanol that volumetric concentration is 95% to concentration of alcohol to be 60～75%,
It is put in 4～8 DEG C after stirring and stands 12～48 hours, filter, filtrate recycling ethanol, obtain health care tea extract, with sucrose, add
Water, sterilizing, make health drink.
2. health drink as claimed in claim 1 is it is characterised in that this health drink is made up of following methods：By prescription weight
Proportioning takes pilea mongolica, Ailanthus altissima flower, plus 10 times of decoctings boil 1.5 hours, and the dregs of a decoction add 6 times amount decoctings again and boil 1 hour, decocting twice
Liquid merges, and filters, and filtrate concentrates, cooling, adds the ethanol that volumetric concentration is 95% to be 70% to concentration of alcohol, is put in 5 after stirring
DEG C standing 24 hours, filter, filtrate recycling ethanol, obtain health care tea extract, with sucrose, add water, sterilizing, make health beverage
Material.
3. the quality determining method of health drink as claimed in claim 1 is it is characterised in that this health drink adopts following sides
Method carries out quality testing：Using high effective liquid chromatography for measuring malvidin content：
（1）Chromatographic condition：Chromatographic column is C18 chromatographic column；With ratio for 40～60：40～60 methyl alcohol -0.15% phosphoric acid solution is
Mobile phase；Detection wavelength 300～330nm, 25～45 DEG C of column temperature；
（2）The preparation of reference substance solution：Precision weighs malvidin reference substance and is placed in volumetric flask, plus methyl alcohol dissolves and is diluted to
Scale, shakes up；Pipette above-mentioned solution and put volumetric flask, plus methanol constant volume, as reference substance stock solution；
（3）The preparation of need testing solution：Take health drink of the present invention, precision weighing, put in conical flask with cover, accurate addition first
Alcohol, close plug, weighed weight, ultrasonically treated, let cool, more weighed weight, supply the weight of less loss with methyl alcohol, shake up, miillpore filter
Filter, take subsequent filtrate, obtain final product need testing solution；
（4）Measure：Precision measures above-mentioned need testing solution, each 5～20 μ L of reference substance solution respectively, injects high performance liquid chromatography
Instrument, is detected.
4. the quality determining method of health drink as claimed in claim 3 is it is characterised in that this health drink adopts following sides
Method carries out quality testing：Using high effective liquid chromatography for measuring malvidin content：
（1）Chromatographic condition：Chromatographic column is C18 chromatographic column；With ratio for 58：42 methyl alcohol -0.15% phosphoric acid solution is mobile phase；
Detection wavelength 328nm, 32 DEG C of column temperature；Number of theoretical plate is calculated by malvidin peak and should be not less than 3000；
（2）The preparation of reference substance solution：Precision weighs malvidin reference substance 10.00mg and is placed in 100mL volumetric flask, plus methyl alcohol
Dissolve and be diluted to scale, shake up；Pipette above-mentioned solution 10mL and put 25mL volumetric flask, plus methanol constant volume, stock as reference substance
Liquid；
（3）The preparation of need testing solution：Take health drink 10mL of the present invention, precision weighing, put in conical flask with cover, accurate addition
Methyl alcohol 10mL, close plug, weighed weight, ultrasonically treated 30min, let cool, more weighed weight, supply the weight of less loss with methyl alcohol, shake
Even, 0.45 μm of filtering with microporous membrane, take subsequent filtrate, obtain final product need testing solution；
（4）Measure：Precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, injects high performance liquid chromatograph, enters
Row detection.
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| CN107693636A (en) * | 2017-10-31 | 2018-02-16 | 黑龙江民族职业学院 | A kind of Chinese medicine composition for treating insomnia and preparation method thereof |
| CN107693636B (en) * | 2017-10-31 | 2021-06-22 | 黑龙江民族职业学院 | Traditional Chinese medicine composition for treating insomnia and preparation method thereof |
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