CN105158111A - Method for determining fat through acid hydrolysis - Google Patents
Method for determining fat through acid hydrolysis Download PDFInfo
- Publication number
- CN105158111A CN105158111A CN201510567044.7A CN201510567044A CN105158111A CN 105158111 A CN105158111 A CN 105158111A CN 201510567044 A CN201510567044 A CN 201510567044A CN 105158111 A CN105158111 A CN 105158111A
- Authority
- CN
- China
- Prior art keywords
- acid hydrolysis
- fat
- ether
- measuring cylinder
- putting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fats And Perfumes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a method for determining fat through acid hydrolysis. The method for determining fat through acid hydrolysis is innovatively characterized in that the method comprises the following steps: 1) taking and putting a sample in a 100mL measuring cylinder with a stopper, then adding 10mL of hydrochloric acid and performing uniform mixing; 2) putting the measuring cylinder with the stopper in a 70 DEG C water bath for digestion for 60min; 3) taking out the measuring cylinder with the stopper, adding 10mL of ethanol, adding 25mL of ethyl ether, oscillating for 1min, opening the measuring cylinder for exhausting gas, plugging and standing the measuring cylinder for 10min, then opening the stopper, and washing fat at the mouth of the measuring cylinder by using petroleum ether-ethyl ether mixed solution; standing for 20min, and recording the volume of the ether layer at the upper layer after layering; 4) taking and putting 25mL supernatant in a conical flask, putting the conical flask in a water batch for drying through evaporation, then putting the conical flask in 100 DEG C drying oven for drying for 2h, taking out the conical flask, putting the conical flask in a drier for cooling for 0.5h, and weighing acid to constant weight. The method for determining fat through acid hydrolysis has the advantages that the operation is facilitated by adopting the measuring cylinder with the stopper, the times of transfer in the process are reduced and the recording of the volume of the ether layer is facilitated; the misoperation caused by taking out all liquid of the ether layer in the international practice can be effectively avoided by taking out 25mL of supernatant, and the error of determination is effectively avoided.
Description
Technical field
The present invention relates to the assay method of fat, be specifically related to the method that acid hydrolysis measures fat.
Background technology
Most of animal and plant food all contains natural fat or lipid, but content is different.Fat in food mainly comprises fat (triglyceride) and lipid (fatty acid, sugar ester and sterol).Fat is the nutrient in food with highest energy, is also one of three nutritious elements in food, and in food, the height of fat content is one of index weighing food value.In addition, in food processing and production process, the lipid content of the raw material of processing, semi-manufacture, finished product all has material impact to the local flavor of food, institutional framework, quality, outward appearance and mouthfeel etc.
The mensuration of fat in food, what great majority adopted is method in international GB5009.6, and i.e. " soxhlet extraction methods ", it is not too convenient to do, water wasting, take electric, time-consuming; Take the second method in international GB5009.6 i.e. " acid-hydrolysis method " to measure fat, water-saving, power saving, save time, but still there is the defect just doing inconvenience, in test process, test specimens transfer number is too much, easily causes experimental error.
Summary of the invention
The technical problem to be solved in the present invention is to provide the method can effectively avoiding the acid hydrolysis of error to measure fat.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
Acid hydrolysis measures the method for fat, and comprise sample preparation, acid hydrolysis and extraction and weighing stage, its innovative point is: adopt tool plug tube to carry out acid hydrolysis test fat content to sample;
Described sample preparation: sample thief is placed in the tool plug graduated cylinder of 100mL, adds 8mL water, then add the mixing of 10mL hydrochloric acid;
Described acid hydrolysis step: tool plug graduated cylinder is put into 70 DEG C of water-baths, digestion 60min;
Described extraction step: take out tool plug graduated cylinder, add 10mL ethanol, add 25mL ether, exitting in the jolting 1min room of opening, is stoppered standing 10min and opens plug, rinses nozzle fat with sherwood oil-ether mixed liquor; Leave standstill 20min, after closing layer, record the volume of upper strata ether layer;
Described oven dry, weighing stage: get 25mL supernatant in conical flask, be placed in water-bath and be put in 100 DEG C of dry 2h of baking oven after evaporate to dryness, takes out and put cooling 0.5h in exsiccator into, repeatedly weigh to constant weight.
Further, the computing method of fat content are as follows:
Further, the weight taking solid sample in described step (1) is 2.00g, and the volume of imbitition is 10mL.
Further, the volume ratio of described step (3) sherwood oil-ether mixed liquor PetroChina Company Limited.'s ether and ether is 1: 1.
Beneficial effect of the present invention: compared with prior art, tool of the present invention has the following advantages:
(1) adopt tool plug graduated cylinder handled easily, process transfer number is reduced, convenient record ether layer volume;
(2) get 25mL supernatant and effectively can avoid whole misoperation of taking out ether layer and causing in GB, effectively avoid the error measured.
Embodiment
Below in conjunction with specific embodiment, technical scheme of the present invention is elaborated.
Embodiment 1
Take cracknel 2.0590g, place tool plug graduated cylinder, add 8mL water, add the mixing of 10mL hydrochloric acid again, place 70 DEG C of water-baths, digestion 60min, takes out tool plug graduated cylinder, adds 10mL ethanol, add 25mL ether, exitting in the jolting 1min room of opening, is stoppered standing 10min and opens plug, rinses nozzle fat with sherwood oil-ether (1: 1) mixed liquor, after standing 20min records upper strata ether layer volume after conjunction layer, get 25mL supernatant in conical flask, after being placed in water-soluble upper evaporate to dryness, be put in 100 DEG C of dry 2h of baking oven, take out and put exsiccator interior cooling 0.5h weighing acid to constant weight.
Record ether layer volume 45.00mL, after conical flask and samples dried, quality is 37.8306g, and conical flask quality is 37.6926g, according to formula
Embodiment 2
Take liquor sample fruit-vegetable juice beverage 10.1242g, place tool plug graduated cylinder, add the mixing of 10mL hydrochloric acid again, place 70 DEG C of water-baths, digestion 60min, take out tool plug graduated cylinder, adding 10mL ethanol, add 25mL ether, exits in the jolting 1min room of opening, be stoppered standing 10min and open plug, rinse nozzle fat with sherwood oil-ether (1: 1) mixed liquor, standing 20min gets 25mL supernatant in conical flask record upper strata ether layer volume after conjunction layer after, be put in 100 DEG C of dry 2h of baking oven after being placed in water-soluble upper evaporate to dryness, take out and put exsiccator interior cooling 0.5h weighing acid to constant weight.
Record ether layer volume 46.00mL, after conical flask and samples dried, quality is 38.1106g, and conical flask quality is 38.0044g, according to formula
Above-described embodiment is only in order to illustrate technical scheme of the present invention; but not design of the present invention and protection domain are limited; those of ordinary skill in the art modifies to technical scheme of the present invention or equivalent replacement; and not departing from aim and the scope of technical scheme, it all should be encompassed in right of the present invention.
Claims (4)
1. acid hydrolysis measures the method for fat, comprises sample preparation, acid hydrolysis and extraction and weighing stage, it is characterized in that: adopt tool plug tube to carry out acid hydrolysis test fat content to sample;
Described sample preparation: sample thief is placed in the tool plug graduated cylinder of 100mL, adds 8mL water, then add the mixing of 10mL hydrochloric acid;
Described acid hydrolysis step: tool plug graduated cylinder is put into 70 DEG C of water-baths, digestion 60min;
Described extraction step: take out tool plug graduated cylinder, add 10mL ethanol, add 25mL ether, exitting in the jolting 1min room of opening, is stoppered standing 10min and opens plug, rinses nozzle fat with sherwood oil-ether mixed liquor; Leave standstill 20min, after closing layer, record the volume of upper strata ether layer;
Described oven dry, weighing stage: get 25mL supernatant in conical flask, be placed in water-bath and be put in 100 DEG C of dry 2h of baking oven after evaporate to dryness, takes out and put cooling 0.5h in exsiccator into, repeatedly weigh to constant weight.
2. acid hydrolysis measures the method for fat, comprises sample preparation, acid hydrolysis and extraction and weighing stage, it is characterized in that: the computing method of fat content are as follows:
3. acid hydrolysis according to claim 1 measures the method for fat, and it is characterized in that: the weight taking solid sample in described step (1) is 2.00g, is accurate to 0.0001g, the weight taking liquid is 10.00g, is accurate to 0.0001g.
4. acid hydrolysis according to claim 1 measures the method for fat, it is characterized in that: the volume ratio of described step (3) sherwood oil-ether mixed liquor PetroChina Company Limited.'s ether and ether is 1: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510567044.7A CN105158111A (en) | 2015-09-08 | 2015-09-08 | Method for determining fat through acid hydrolysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510567044.7A CN105158111A (en) | 2015-09-08 | 2015-09-08 | Method for determining fat through acid hydrolysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105158111A true CN105158111A (en) | 2015-12-16 |
Family
ID=54799047
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510567044.7A Pending CN105158111A (en) | 2015-09-08 | 2015-09-08 | Method for determining fat through acid hydrolysis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105158111A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105928821A (en) * | 2016-07-13 | 2016-09-07 | 南通中兴多元复合钢管有限公司 | Method for determining fat through acid hydrolysis |
| CN110470755A (en) * | 2019-08-09 | 2019-11-19 | 凭祥出入境检验检疫局综合技术服务中心 | A kind of method of quick detection Trans-fatty Acids in Foods |
| CN112067492A (en) * | 2020-07-13 | 2020-12-11 | 内蒙古沙漠之神生物科技有限公司 | Camel milk component detection method |
-
2015
- 2015-09-08 CN CN201510567044.7A patent/CN105158111A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105928821A (en) * | 2016-07-13 | 2016-09-07 | 南通中兴多元复合钢管有限公司 | Method for determining fat through acid hydrolysis |
| CN110470755A (en) * | 2019-08-09 | 2019-11-19 | 凭祥出入境检验检疫局综合技术服务中心 | A kind of method of quick detection Trans-fatty Acids in Foods |
| CN112067492A (en) * | 2020-07-13 | 2020-12-11 | 内蒙古沙漠之神生物科技有限公司 | Camel milk component detection method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103149114B (en) | Measurement method of coating rate of paper-making reconstituted tobacco | |
| CN105158111A (en) | Method for determining fat through acid hydrolysis | |
| CN208818602U (en) | A kind of Ceramic Tiles water absorption rate test tooling | |
| CN103558180A (en) | Method for quickly detecting physicochemical indexes in saccharification leavening agents through near infrared | |
| CN103760058A (en) | Method for determining fat by acid hydrolysis | |
| CN104055838A (en) | Extraction method of walnut green seedcase polyphenol | |
| CN101480213B (en) | Technique for processing conserved blueberry fruit | |
| CN105907531A (en) | Wine containing rhizoma polygonatum and fructus lycii, and production technology of wine | |
| CN201837605U (en) | Hydration temperature-rise tester for concrete | |
| CN105243599A (en) | Method for building rice storage quality evaluation model | |
| CN106754048A (en) | A kind of brewing method of Gentiana triflora banana compound fruit wine | |
| CN105316206A (en) | Brewing method of pueraria lobata vinegar | |
| CN104849166A (en) | Method for detecting content of alpha cellulose in alkali cellulose | |
| CN103115842B (en) | Method for measuring recycled stem backfilling rate | |
| CN105928821A (en) | Method for determining fat through acid hydrolysis | |
| CN104872781B (en) | The solar energy drying method of scallop post | |
| CN110467993A (en) | A kind of model for evaluating grain degree of cooking in Maotai-flavor liquor production process | |
| CN105954420B (en) | The rapid detection method of ligustrazine in a kind of 8 ° of face cream vinegar | |
| CN204902542U (en) | Novel tealeaves that can weigh is dried device | |
| CN101875930B (en) | Method for rapidly and efficiently extracting total RNA of rice tissue | |
| CN205404581U (en) | A instrument for experimental usefulness of wood -based plate | |
| CN113310782A (en) | Digestion method for determining selenium in agricultural products and determination method for selenium content in agricultural products | |
| CN102539364B (en) | Method for detecting rice maturity | |
| CN110749668A (en) | Marker and method for screening high-aroma tea tree resource suitable for oolong tea | |
| CN202340747U (en) | Low-temperature vacuum drying equipment for tea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151216 |
|
| WD01 | Invention patent application deemed withdrawn after publication |