CN105158024A - Negative pressure sample feeding instrument and negative pressure sample feeding method for biochemical analysis and identification plate - Google Patents
Negative pressure sample feeding instrument and negative pressure sample feeding method for biochemical analysis and identification plate Download PDFInfo
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- CN105158024A CN105158024A CN201510582239.9A CN201510582239A CN105158024A CN 105158024 A CN105158024 A CN 105158024A CN 201510582239 A CN201510582239 A CN 201510582239A CN 105158024 A CN105158024 A CN 105158024A
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- identification plate
- negative pressure
- cavity
- biochemical analysis
- sample feeding
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Abstract
The invention discloses a negative pressure sample feeding instrument and a negative pressure sample feeding method for a biochemical analysis and identification plate. The negative pressure sample feeding instrument comprises a vacuum chamber, a storage rack, and an identification plate and a liquid bottle arranged on the storage rack, wherein the identification plate is communicated with bacterium suspension in the liquid bottle by a slime tube; the vacuum chamber comprises a vacuum pump, a gas inlet valve, a pressure sensor and a chamber for placing the storage rack; the vacuum pump and the gas inlet valve are communicated with the chamber by a pipeline, and the pressure sensor is used for measuring gas pressure in the chamber. According to the sample feeding instrument and method, negative pressure can be formed in a cultivation hole in the identification plate by the vacuum chamber, partial bacterium suspension in the liquid bottle can be sucked into the identification plate by utilizing the negative pressure, the work of feeding bacterium suspension of different types for multiple identification plates can be completed by using just tens of seconds, the sample feeding efficiency is high and the operation is safe.
Description
Technical field
The present invention relates to identification plate loading techniques field, particularly relate to a kind of biochemical analysis identification plate negative pressure application of sample instrument and method.
Background technology
Existing identification plate loading methods utilizes mechanical motion and electric piston to be dropwise sequentially added in identification plate by bacteria suspension, the maximum shortcoming of this method is that feed rate is slow, and single application of sample one can only hang bacterium liquid, efficiency is very low, and mechanical moving element complex structure and with high costs, be inconvenient to safeguard, in addition, when titration, bacteria suspension also easily spills, safe not.
Summary of the invention
In order to overcome above-mentioned the deficiencies in the prior art, the invention provides a kind of biochemical analysis identification plate negative pressure application of sample instrument and method, effectively can improve feed rate, apparatus structure is simple, handling safety.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of biochemical analysis identification plate negative pressure application of sample instrument, comprise vacuum tank, rack, be placed on identification plate on rack and liquid bottles, described identification plate is connected with the bacteria suspension be contained in liquid bottles by tubule, described vacuum tank comprises the cavity of vacuum pump, gas admittance valve, pressure transducer and accommodating rack, described vacuum pump is connected with cavity by pipeline with gas admittance valve, and the air pressure in cavity measured by described pressure transducer.
As the improvement of technique scheme, be provided with some culture hole be interconnected in described identification plate, described some culture hole communicate with the external world by means of only tubule.
As the improvement of technique scheme, between described gas admittance valve and cavity, be also provided with air strainer.
As the improvement of technique scheme, described vacuum tank also comprises chamber door, and the outer ring, inner side of described chamber door is provided with sealing strip.
A kind of biochemical analysis identification plate negative pressure loading methods, the method specifically comprises the following steps:
(1) will the identification plate of application of sample be needed to be placed on rack with the liquid bottles that bacteria suspension is housed, identification plate inserts in the bacteria suspension in liquid bottles by tubule;
(2) rack is placed in the cavity of vacuum tank, fastens chamber door, seal chamber;
(3) open vacuum pump to bleed to cavity, and measure the air pressure in cavity by pressure transducer, until reach vacuum state in cavity, make the culture hole in identification plate form negative pressure;
(4) open gas admittance valve, make outside air slowly enter cavity after air strainer, until the air pressure in cavity is identical with external atmosphere pressure, the part bacteria suspension suck-back in liquid bottles enters in culture hole by the negative pressure in culture hole;
(5) open chamber door, the identification plate in cavity and liquid bottles are taken out, obtains the identification plate being full of bacteria suspension.
As the improvement of technique scheme, atmospheric pressure value when reaching vacuum state in described cavity is 680 ~ 700mmHg.
Beneficial effect of the present invention has:
This application of sample instrument and method make the culture hole in identification plate form negative pressure by vacuum tank, recycling this negative pressure enters in identification plate by the part bacteria suspension suck-back in liquid bottles, only need just to complete the work to the dissimilar bacteria suspension of polylith identification plate difference application of sample tens of second, application of sample efficiency is very high, operates also very safe.
Accompanying drawing explanation
Below in conjunction with drawings and the specific embodiments, the invention will be further described, wherein:
Fig. 1 is the structural representation of rack in the embodiment of the present invention;
Fig. 2 is the structural representation of vacuum tank in the embodiment of the present invention;
Fig. 3 is the structural representation one of identification plate in the embodiment of the present invention;
Fig. 4 is the structural representation two of identification plate in the embodiment of the present invention.
Embodiment
See Fig. 1, Fig. 3 and Fig. 4, a kind of biochemical analysis identification plate negative pressure application of sample instrument of the present invention, comprise vacuum tank, rack 1, be placed on identification plate 2 on rack 1 and liquid bottles 3, identification plate 2 is connected with the bacteria suspension be contained in liquid bottles 3 by tubule 4, in the present embodiment, identification plate 2, liquid bottles 3 and tubule 4 are set to ten groups, be provided with some culture hole 9 be interconnected in identification plate 2, some culture hole 9 communicate with the external world by means of only tubule 4.
With further reference to Fig. 2, vacuum tank comprises the cavity 8 of vacuum pump 5, gas admittance valve 6, pressure transducer 7, chamber door 11 and accommodating rack 1, vacuum pump 5 is connected with cavity 8 by pipeline with gas admittance valve 6, the air pressure in cavity 8 measured by pressure transducer 7, also be provided with air strainer 10 between gas admittance valve 6 and cavity 8, the outer ring, inner side of chamber door 11 is provided with sealing strip 12.
In addition, the present invention also provides a kind of biochemical analysis identification plate negative pressure loading methods, and it adopts above-mentioned application of sample instrument, and the method specifically comprises the following steps:
(1) will the identification plate 2 of application of sample be needed to be placed on rack 1 with the liquid bottles 3 that bacteria suspension is housed, identification plate 2 inserts in the bacteria suspension in liquid bottles 3 by tubule 4;
(2) rack 1 is placed in the cavity 8 of vacuum tank, fastens chamber door 11, seal chamber 8;
(3) open vacuum pump 5 pairs of cavitys 8 to bleed, and measure the air pressure in cavity 8 by pressure transducer 7, until reach vacuum state in cavity 8, make the culture hole 9 in identification plate 2 form negative pressure;
(4) open gas admittance valve 6, make outside air slowly enter cavity 8 after air strainer 10, until the air pressure in cavity 8 is identical with external atmosphere pressure, the part bacteria suspension suck-back in liquid bottles 3 enters in culture hole 9 by the negative pressure in culture hole 9;
(5) open chamber door 11, the identification plate 2 in cavity 8 and liquid bottles 3 are taken out, obtain the identification plate 2 being full of bacteria suspension.
More specifically, atmospheric pressure value when reaching vacuum state in cavity 8 is 680 ~ 700mmHg.
This loading methods only needs just to complete the work to the dissimilar bacteria suspension of polylith identification plate 2 difference application of sample tens of second, and application of sample efficiency is very high, operates also very safe.
The above, just better embodiment of the present invention, but the present invention is not limited to above-described embodiment, as long as it reaches technique effect of the present invention with any same or similar means, all should belong to protection scope of the present invention.
Claims (6)
1. a biochemical analysis identification plate negative pressure application of sample instrument, it is characterized in that: comprise vacuum tank, rack, be placed on identification plate on rack and liquid bottles, described identification plate is connected with the bacteria suspension be contained in liquid bottles by tubule, described vacuum tank comprises the cavity of vacuum pump, gas admittance valve, pressure transducer and accommodating rack, described vacuum pump is connected with cavity by pipeline with gas admittance valve, and the air pressure in cavity measured by described pressure transducer.
2. a kind of biochemical analysis identification plate negative pressure application of sample instrument according to claim 1, it is characterized in that: be provided with some culture hole be interconnected in described identification plate, described some culture hole communicate with the external world by means of only tubule.
3. a kind of biochemical analysis identification plate negative pressure application of sample instrument according to claim 1, is characterized in that: be also provided with air strainer between described gas admittance valve and cavity.
4. a kind of biochemical analysis identification plate negative pressure application of sample instrument according to claim 1, it is characterized in that: described vacuum tank also comprises chamber door, the outer ring, inner side of described chamber door is provided with sealing strip.
5. a biochemical analysis identification plate negative pressure loading methods, is characterized in that: described method adopts a kind of biochemical analysis identification plate negative pressure application of sample instrument described in any one of claim 1-4, and the method specifically comprises the following steps:
(1) will the identification plate of application of sample be needed to be placed on rack with the liquid bottles that bacteria suspension is housed, identification plate inserts in the bacteria suspension in liquid bottles by tubule;
(2) rack is placed in the cavity of vacuum tank, fastens chamber door, seal chamber;
(3) open vacuum pump to bleed to cavity, and measure the air pressure in cavity by pressure transducer, until reach vacuum state in cavity, make the culture hole in identification plate form negative pressure;
(4) open gas admittance valve, make outside air slowly enter cavity after air strainer, until the air pressure in cavity is identical with external atmosphere pressure, the part bacteria suspension suck-back in liquid bottles enters in culture hole by the negative pressure in culture hole;
(5) open chamber door, the identification plate in cavity and liquid bottles are taken out, obtains the identification plate being full of bacteria suspension.
6. the method for a kind of biochemical analysis identification plate negative pressure application of sample according to claim 5, is characterized in that: atmospheric pressure value when reaching vacuum state in described cavity is 680 ~ 700mmHg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510582239.9A CN105158024A (en) | 2015-09-14 | 2015-09-14 | Negative pressure sample feeding instrument and negative pressure sample feeding method for biochemical analysis and identification plate |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510582239.9A CN105158024A (en) | 2015-09-14 | 2015-09-14 | Negative pressure sample feeding instrument and negative pressure sample feeding method for biochemical analysis and identification plate |
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| CN105158024A true CN105158024A (en) | 2015-12-16 |
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| CN201510582239.9A Pending CN105158024A (en) | 2015-09-14 | 2015-09-14 | Negative pressure sample feeding instrument and negative pressure sample feeding method for biochemical analysis and identification plate |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226555A (en) * | 2018-03-16 | 2018-06-29 | 李涛 | The quick sample adding device of clinical samples and method |
| CN110106072A (en) * | 2019-04-25 | 2019-08-09 | 珠海迪尔生物工程有限公司 | Sample adding device and method for microbial sample test card |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2069110U (en) * | 1990-07-06 | 1991-01-09 | 天津大学 | Automatic sampling apparatus for digestion medical solution |
| CN2735341Y (en) * | 2003-07-02 | 2005-10-19 | 江苏绿叶环保科技仪器有限公司 | Online automatic monitor for chemical oxygen demand |
| JP2007187444A (en) * | 2006-01-11 | 2007-07-26 | Hitachi High-Technologies Corp | Autoanalyzer |
| CN201126435Y (en) * | 2007-11-12 | 2008-10-01 | 江苏绿叶环保科技仪器有限公司 | Ammonia nitrogen automatic detection instrument |
| CN201159728Y (en) * | 2007-11-12 | 2008-12-03 | 江苏绿叶环保科技仪器有限公司 | Automatic detection instrument for permanganate index |
| CN201387392Y (en) * | 2009-03-28 | 2010-01-20 | 王占华 | Negative pressure type specimen sampler |
| CN201999938U (en) * | 2011-03-08 | 2011-10-05 | 史跃杰 | Automatic filling test card for bacteria drug sensitivity test |
| CN203785930U (en) * | 2013-12-07 | 2014-08-20 | 中国人民解放军军事医学科学院军事兽医研究所 | Vacuum type biologically safe sampler |
| CN104266895A (en) * | 2014-10-11 | 2015-01-07 | 沈佳特 | Automatic continuous multi-stage dilution device for bacterial suspension and real-time counting method |
| CN204142554U (en) * | 2014-10-11 | 2015-02-04 | 沈佳特 | A kind of bacteria suspension continuous multi-stage automatic dilution and counting assembly |
| CN204988791U (en) * | 2015-09-14 | 2016-01-20 | 珠海迪尔生物工程有限公司 | Biochemical analysis appraisal board negative pressure application of sample instrument |
-
2015
- 2015-09-14 CN CN201510582239.9A patent/CN105158024A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2069110U (en) * | 1990-07-06 | 1991-01-09 | 天津大学 | Automatic sampling apparatus for digestion medical solution |
| CN2735341Y (en) * | 2003-07-02 | 2005-10-19 | 江苏绿叶环保科技仪器有限公司 | Online automatic monitor for chemical oxygen demand |
| JP2007187444A (en) * | 2006-01-11 | 2007-07-26 | Hitachi High-Technologies Corp | Autoanalyzer |
| CN201126435Y (en) * | 2007-11-12 | 2008-10-01 | 江苏绿叶环保科技仪器有限公司 | Ammonia nitrogen automatic detection instrument |
| CN201159728Y (en) * | 2007-11-12 | 2008-12-03 | 江苏绿叶环保科技仪器有限公司 | Automatic detection instrument for permanganate index |
| CN201387392Y (en) * | 2009-03-28 | 2010-01-20 | 王占华 | Negative pressure type specimen sampler |
| CN201999938U (en) * | 2011-03-08 | 2011-10-05 | 史跃杰 | Automatic filling test card for bacteria drug sensitivity test |
| CN203785930U (en) * | 2013-12-07 | 2014-08-20 | 中国人民解放军军事医学科学院军事兽医研究所 | Vacuum type biologically safe sampler |
| CN104266895A (en) * | 2014-10-11 | 2015-01-07 | 沈佳特 | Automatic continuous multi-stage dilution device for bacterial suspension and real-time counting method |
| CN204142554U (en) * | 2014-10-11 | 2015-02-04 | 沈佳特 | A kind of bacteria suspension continuous multi-stage automatic dilution and counting assembly |
| CN204988791U (en) * | 2015-09-14 | 2016-01-20 | 珠海迪尔生物工程有限公司 | Biochemical analysis appraisal board negative pressure application of sample instrument |
Non-Patent Citations (3)
| Title |
|---|
| 刘志刚: "多孔板进样器的研究与开发", 《中国优秀硕士学位论文全文数据库 工程科技Ⅱ辑》 * |
| 民航无损检测人员资格鉴定与认证委员会: "《航空器渗透检测》", 31 March 2014, 中国民航出版社 * |
| 陈铁龙: "自动液体加样仪系统的研制", 《中国优秀硕士学位论文全文数据库 工程科技Ⅱ辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226555A (en) * | 2018-03-16 | 2018-06-29 | 李涛 | The quick sample adding device of clinical samples and method |
| CN110106072A (en) * | 2019-04-25 | 2019-08-09 | 珠海迪尔生物工程有限公司 | Sample adding device and method for microbial sample test card |
| CN110106072B (en) * | 2019-04-25 | 2022-05-13 | 珠海迪尔生物工程股份有限公司 | Sample adding device and method for microbial sample test card |
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Application publication date: 20151216 |