CN105154207A - Method for extracting moringa oil from moringa seeds - Google Patents
Method for extracting moringa oil from moringa seeds Download PDFInfo
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- CN105154207A CN105154207A CN201510481582.4A CN201510481582A CN105154207A CN 105154207 A CN105154207 A CN 105154207A CN 201510481582 A CN201510481582 A CN 201510481582A CN 105154207 A CN105154207 A CN 105154207A
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- moringa
- moringa seed
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- enzymolysis
- oil ben
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Abstract
The invention discloses a method for extracting moringa oil from moringa seeds. The method comprises steps as follows: (1) raw materials are crushed: the moringa seeds are collected, dried, crushed and sieved, and moringa seed powder is obtained; (2) enzymolysis is performed: the moringa seed powder is mixed with water, combined enzymes with the mass accounting for 0.01%-0.08% of the weight of the moringa seed powder are added, pH is regulated to range from 3 to 5, and an enzymolysis solution is obtained through enzymolysis in a water bath at the temperature of 35-50 DEG C for 2-5 h, wherein the combined enzymes comprise protease and cellulase in the weight ratio being (2-5):(1-4); (3) the moringa oil is obtained through centrifugal separation of the enzymolysis solution. The moringa oil is extracted from the moringa seeds with an aqueous enzymatic method, the combined enzymes comprising the protease and the cellulase are selected for enzymolysis of the moringa seeds, the yield of the moringa oil can be higher than 70%, and the method is simple, easy to implement and applicable to large-scale industrial production.
Description
Technical field
The invention belongs to plant component extraction technical field, be specifically related to a kind of method extracting oil ben from Moringa seed.
Background technology
Moringa is set also known as drumstick, belong to Moringaceae Moringa plant, Moringa is a kind of torrid zone, subtropics multi-functional plant of mystery, there is long edible history, nutritious and comprehensive, be the best various nutrients such as vegetable-protein, VITAMIN, folic acid, pantothenic acid, calcium, iron, the selenium source found at present.
Moringa originates in north India, known in the world have more than ten kind of kind, except root crust is because having two kinds of alkaloids not except edible, rich in protein, VITAMIN and amino acid are contained in other position, great help is had to vegetarian, calcareous contained by it is four times of milk, protein is two times of milk, potassium is three times of banana, iron is three times of spinach, and vitamins C is seven times of oranges and tangerines, and vitamin A (beta carotene) is four times of Radix Dauci Sativae, therefore Moringa is the underfed heath food of antagonism, is described as the tree of miracle by western scientist.
Oil ben is one of main component of Moringa, and a kind of xylophyta oil of oil ben, its trade(brand)name is called " this oil " or " ben-oil ".Compared with other vegetables oil, oil ben has unique characteristic, and that is exactly excellent oxidation-resistance, do not need to add any synergistic agent or sanitas in its storage, oil ben is preserved at normal temperatures and is never degenerated for about 1 year, does not become sour, can not produce rancidity.Oil ben has been widely used in many industries such as food, makeup, machinofacture and space flight, but the extracting method very complicated of current oil ben, and also the oil ben purity obtained is lower.
Summary of the invention
The invention provides a kind of method extracting oil ben from Moringa seed, the method is simple, production of easily magnifying, and can extract the high-quality oil ben of high purity, can be used for industrialized scale operation.
From Moringa seed, extract a method for oil ben, comprise the following steps:
(1) raw material pulverizing: gather Moringa seed, drying, pulverizes and sieves and obtain Moringa seed powder;
(2) enzymolysis: mixed with water by Moringa seed powder, adds the combination enzyme that quality accounts for Moringa seed grain weight amount 0.01 ~ 0.08%, and regulate pH to 3 ~ 5, finally in 35 ~ 50 DEG C of water-baths, enzymolysis 2 ~ 5h obtains enzymolysis solution;
Wherein said combination enzyme is proteolytic enzyme and cellulase, and the weight ratio of described proteolytic enzyme and cellulase is 2 ~ 5:1 ~ 4;
In the present invention, before Moringa seed powder mixes with water, first Moringa seed powder can be heated under the condition of 30 ~ 40 DEG C, can better oil ben be extracted, and improve the purity of oil.Also can reduce the consumption of enzyme.
(3) enzymolysis solution centrifugation is obtained described oil ben.
The present invention selects aqueous enzymatic method to extract oil ben from Moringa seed, and select using the combination enzyme of proteolytic enzyme and cellulase composition as the enzyme of enzymolysis Moringa seed, combination enzyme can destroy cell walls, the material such as polysaccharide and protein is decomposed, the mobility of oil in oil plant tissue can be increased, thus improve oil yield.
In order to improve the enzymolysis of enzyme to Moringa seed powder, as preferably, the order number of described Moringa seed powder is 200 ~ 400 orders.
As more preferably, the order number of described Moringa seed powder is 300 orders.
As preferably, the weight ratio of described proteolytic enzyme and cellulase is 3:4.
As preferably, the weight that described combination enzyme accounts for Moringa seed powder is 0.03 ~ 0.05%.
As preferably, the weight that described combination enzyme accounts for Moringa seed powder is 0.04%.
In described step (3), enzymolysis solution centrifugal 6 ~ 15min under rotating speed 2500 ~ 5000r/min is obtained described oil ben.
Compared with prior art, beneficial effect of the present invention is embodied in:
The present invention selects aqueous enzymatic method to extract oil ben from Moringa seed, and select using the combination enzyme of proteolytic enzyme and cellulase composition as the enzyme of enzymolysis Moringa seed, the yield of oil ben can up to more than 70%, method is simple, easy extension is produced, and the high-quality oil ben of high purity can be extracted, can be used for industrialized scale operation.
Embodiment
Embodiment 1
(1) raw material pulverizing: gather Moringa seed, drying, pulverizes and sieves and obtain 300 object Moringa seed powder;
(2) enzymolysis: after Moringa seed powder is heated 2 hours under the condition of 30 DEG C, mix with water, add the combination enzyme that quality accounts for Moringa seed grain weight amount 0.04%, regulate pH to 5, finally in 40 DEG C of water-baths, enzymolysis 4h obtains enzymolysis solution;
Wherein combining enzyme is proteolytic enzyme and cellulase, and the weight ratio of proteolytic enzyme and cellulase is 3:4;
(3) enzymolysis solution centrifugal 10min under rotating speed 5000r/min is obtained described oil ben.
Embodiment 2
(1) raw material pulverizing: gather Moringa seed, drying, pulverizes and sieves and obtain 200 object Moringa seed powder;
(2) enzymolysis: after Moringa seed powder is heated 3 hours under the condition of 35 DEG C, mix with water, add the combination enzyme that quality accounts for Moringa seed grain weight amount 0.03%, regulate pH to 3, finally in 50 DEG C of water-baths, enzymolysis 2h obtains enzymolysis solution;
Wherein combining enzyme is proteolytic enzyme and cellulase, and the weight ratio of proteolytic enzyme and cellulase is 2:1;
(3) enzymolysis solution centrifugal 15min under rotating speed 2500r/min is obtained described oil ben.
Embodiment 3
(1) raw material pulverizing: gather Moringa seed, drying, pulverizes and sieves and obtain 400 object Moringa seed powder;
(2) enzymolysis: after Moringa seed powder is heated 1 hour under the condition of 40 DEG C, mix with water, add the combination enzyme that quality accounts for Moringa seed grain weight amount 0.08%, regulate pH to 5, finally in 40 DEG C of water-baths, enzymolysis 4h obtains enzymolysis solution;
Wherein combining enzyme is proteolytic enzyme and cellulase, and the weight ratio of proteolytic enzyme and cellulase is 3:4;
(3) enzymolysis solution centrifugal 6min under rotating speed 5000r/min is obtained described oil ben.
Embodiment 4
(1) raw material pulverizing: gather Moringa seed, drying, pulverizes and sieves and obtain 300 object Moringa seed powder;
(2) enzymolysis: after Moringa seed powder is heated 2.5 hours under the condition of 36 DEG C, mix with water, add the combination enzyme that quality accounts for Moringa seed grain weight amount 0.05%, regulate pH to 5, finally in 40 DEG C of water-baths, enzymolysis 4h obtains enzymolysis solution;
Wherein combining enzyme is proteolytic enzyme and cellulase, and the weight ratio of proteolytic enzyme and cellulase is 5:4;
(3) enzymolysis solution centrifugal 10min under rotating speed 5000r/min is obtained described oil ben.
Embodiment 5
(1) raw material pulverizing: gather Moringa seed, drying, pulverizes and sieves and obtain 300 object Moringa seed powder;
(2) enzymolysis: after Moringa seed powder is heated 2 hours under the condition of 37 DEG C, mix with water, add the combination enzyme that quality accounts for Moringa seed grain weight amount 0.01%, regulate pH to 3, finally in 35 DEG C of water-baths, enzymolysis 5h obtains enzymolysis solution;
Wherein combining enzyme is proteolytic enzyme and cellulase, and the weight ratio of proteolytic enzyme and cellulase is 3:4;
(3) enzymolysis solution centrifugal 10min under rotating speed 5000r/min is obtained described oil ben.
The yield of the method gained oil ben of embodiment 1 ~ 5 is in table 1.
Table 1 extracts the yield of oil ben from Moringa seed
Claims (7)
1. from Moringa seed, extract a method for oil ben, it is characterized in that, comprise the following steps:
(1) raw material pulverizing: gather Moringa seed, drying, pulverizes and sieves and obtain Moringa seed powder;
(2) enzymolysis: mixed with water by Moringa seed powder, adds the combination enzyme that quality accounts for Moringa seed grain weight amount 0.01 ~ 0.08%, and regulate pH to 3 ~ 5, finally in 35 ~ 50 DEG C of water-baths, enzymolysis 2 ~ 5h obtains enzymolysis solution;
Wherein said combination enzyme is proteolytic enzyme and cellulase, and the weight ratio of described proteolytic enzyme and cellulase is 2 ~ 5:1 ~ 4;
(3) enzymolysis solution centrifugation is obtained described oil ben.
2. from Moringa seed, extract the method for oil ben as claimed in claim 1, it is characterized in that, the order number of described Moringa seed powder is 200 ~ 400 orders.
3. from Moringa seed, extract the method for oil ben as claimed in claim 2, it is characterized in that, the order number of described Moringa seed powder is 300 orders.
4. from Moringa seed, extract the method for oil ben as claimed in claim 1, it is characterized in that, the weight ratio of described proteolytic enzyme and cellulase is 3:4.
5. from Moringa seed, extract the method for oil ben as claimed in claim 1, it is characterized in that, the weight that described combination enzyme accounts for Moringa seed powder is 0.03 ~ 0.05%.
6. extract the method for oil ben as claimed in claim 5 from Moringa seed kind, it is characterized in that, the weight that described combination enzyme accounts for Moringa seed powder is 0.04%.
7. from Moringa seed, extract the method for oil ben as claimed in claim 1, it is characterized in that, in described step (3), enzymolysis solution centrifugal 6 ~ 15min under rotating speed 2500 ~ 5000r/min is obtained described oil ben.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510481582.4A CN105154207A (en) | 2015-08-03 | 2015-08-03 | Method for extracting moringa oil from moringa seeds |
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| CN201510481582.4A CN105154207A (en) | 2015-08-03 | 2015-08-03 | Method for extracting moringa oil from moringa seeds |
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| CN201510481582.4A Pending CN105154207A (en) | 2015-08-03 | 2015-08-03 | Method for extracting moringa oil from moringa seeds |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875867A (en) * | 2016-04-29 | 2016-08-24 | 安徽恋尚你食品有限公司 | Moringa oleifera edible oil |
| CN106306936A (en) * | 2016-08-17 | 2017-01-11 | 深圳百绿盛农业科技发展有限公司 | Preparation method of moringa oleifera enzymolyzed drink |
| CN107586303A (en) * | 2017-11-03 | 2018-01-16 | 隆安县荣胜养殖有限公司 | Method for extracting and purifying moringa oleifera from moringa oleifera roots |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294118A (en) * | 2008-06-24 | 2008-10-29 | 中国林业科学研究院资源昆虫研究所 | Method for abstracting ben oil from horseradish tree return meal after press |
| CN101508936A (en) * | 2009-03-19 | 2009-08-19 | 中国林业科学研究院资源昆虫研究所 | Abstraction method for ben oil |
| CN101508729A (en) * | 2009-03-19 | 2009-08-19 | 中国林业科学研究院资源昆虫研究所 | Method for extracting horseradish tree protein from horseradish tree seed |
-
2015
- 2015-08-03 CN CN201510481582.4A patent/CN105154207A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294118A (en) * | 2008-06-24 | 2008-10-29 | 中国林业科学研究院资源昆虫研究所 | Method for abstracting ben oil from horseradish tree return meal after press |
| CN101508936A (en) * | 2009-03-19 | 2009-08-19 | 中国林业科学研究院资源昆虫研究所 | Abstraction method for ben oil |
| CN101508729A (en) * | 2009-03-19 | 2009-08-19 | 中国林业科学研究院资源昆虫研究所 | Method for extracting horseradish tree protein from horseradish tree seed |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875867A (en) * | 2016-04-29 | 2016-08-24 | 安徽恋尚你食品有限公司 | Moringa oleifera edible oil |
| CN106306936A (en) * | 2016-08-17 | 2017-01-11 | 深圳百绿盛农业科技发展有限公司 | Preparation method of moringa oleifera enzymolyzed drink |
| CN107586303A (en) * | 2017-11-03 | 2018-01-16 | 隆安县荣胜养殖有限公司 | Method for extracting and purifying moringa oleifera from moringa oleifera roots |
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Application publication date: 20151216 |