CN105146745B - Immobilized catalase additive for tobacco as well as preparation method and application of additive - Google Patents
Immobilized catalase additive for tobacco as well as preparation method and application of additive Download PDFInfo
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- CN105146745B CN105146745B CN201510500332.0A CN201510500332A CN105146745B CN 105146745 B CN105146745 B CN 105146745B CN 201510500332 A CN201510500332 A CN 201510500332A CN 105146745 B CN105146745 B CN 105146745B
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Abstract
The invention relates to the field of the preparation of tobacco, and in particular relates to an immobilized catalase additive for tobacco as well as a preparation method and application of the additive. In the immobilized catalase additive for tobacco provided by the invention, an immobilized reagent is selected from any one or any combinations of porous starch, chitosan and chitosan derivatives. According to the invention, the catalase, which is processed by the environment-friendly and non-toxic immobilized reagent, is good in stability, capable of effectively removing free radicals in the smoke of cigarettes and reducing harm, and meanwhile, the catalase is capable of protecting the spinnability, the monofilament strength, the ductility and the like of cellulose acetate fiber from being affected.
Description
The application is《A kind of immobilized catalase cigarette additive and its preparation method and application》Division Shen
Please, the applying date of original application：2013.10.18, application number：201310493334.2.
Technical field
The present invention relates to field of tobacco preparation, more particularly to a kind of immobilized catalase cigarette additive and its
Preparation method and application.
Background technology
Find that the free radical in cigarette smoke is give rise to diseases important about smoking and the research of health in recent years
Reason.The direct toxicity of Free Radicals In Cigarette Smoke is the materials such as coup injury cell and enzyme, and combined with DNA make cell transformation and
Produce mutation；Its indirect toxicity is can in vivo to activate and produce other bigger free radicals of a large amount of activity more high toxicities, such as super
Oxygen radical and hydroxyl radical free radical, so as to cause various pathologies.The flue gas of every cigarette produces about 1*1016Individual free radical, flue gas
In free radical be generally divided into two classes, i.e. gaseous phase free radical and grain phase free radical.Gaseous phase free radical property is very active, with alkyl
Based on free radical and alkoxy radical；The property of grain phase free radical is more stable, with quinones and half quinones, condensed-nuclei aromatics type free
Based on base.Research finds that cigarette gaseous phase free radical has stronger oxidisability, and grain phase free radical has compared with strong reducing property；And
Because grain phase free radical is embedded in flue gas grain gathering compound matrix, therefore for the removal of grain phase free radical is depended on
The overall decline of smoke's total particulate matter content, the selective research for reducing free-radical contents in main flume is concentrated mainly on gas
Phase free radical aspect.
The research for dispelling free radical in cigarette smoke achieves many achievements, and such as BJ Tobacco Factory and the Chinese Academy of Sciences is biological
Gaseous phase free radical in cigarette smoke is dispelled using the mixture of green-tea extract and vitamin by physics Institute；Athens, GRE university
The seminar of Stavridis leaders dispel gaseous phase free radical and other harmful substances matter in flue gas with hemoglobin, and using grinding
Study carefully achievement and be respectively produced the cigarette products such as ENJOY, WUYESHENG and BF；Guangzhou legendary god of farming's cigarette Science and Technology Ltd. etc. is according to the traditional Chinese medical science
" legendary god of farming's extract " that pharmaceutical theory is developed reduces the harmful components such as cigarette gaseous phase free radical, TSNAs；Mei Jianhua is invented
A kind of cigarette filter of the suppression NO free radical with Tea Polyphenols and vitamin C as effective ingredient；Zhao Baolu etc. has invented one kind
The cigarette filter of free radical in flue gas can substantially be reduced, additive is PCA, or anthocyanidin is used in mixed way with vitamin C.
Because these technologies adopt antioxidant mostly, and antioxidant dispels effect to free radical after cigarette is placed for a long time
Fruit can be affected, therefore, find new cheap, stable, efficient, sustained release antioxidant and dispel gas phase freedom in cigarette smoke
Base is very important.Catalase is widely present in human body, animal, plant also known as catalase (Catalase, abbreviation CAT)
In microorganism, a large amount of hydrogen peroxide are contained in animal's liver, red blood cell, plant chloroplast and bacterium, fungi, actinomyces etc.
Enzyme.So far catalase is had become in agricultural, and food and Dairy Products Industry Implementing, paper pulp and paper-making industry, agricultural environmental protection industry
There is one of enzyme of using value.Catalase is a homotetramer, and each subunit is residual containing 500 amino acid are had more than
Base, the avtive spot of each subunit contains a porphyrin heme group, for the reaction of catalyzing hydrogen peroxide.Hydrogen peroxide
Enzyme can efficiently catalyzing and oxidizing reduction reaction, be to be currently known most important anti-oxidant barrier in nearly all aerobe body；
Under conditions of having peroxide, the nitrogen oxides in the rapid catalytic degradation flue gas of energy destroys gaseous phase free radical to catalase
Reaction chain, so as to reduce flue gas in gaseous phase free radical content.
The content of the invention
Present invention aim to overcome that defect of the prior art, there is provided in a kind of alternative reduction cigarette mainstream flue gas
The immobilized catalase cigarette additive of free-radical contents.The present invention utilizes enzyme immobilization technology, makes efficient, steady
Fixed, sustained release biology enzyme cigarette additive, biology enzyme selects catalase, fixing agent may be selected porous-starch, shitosan,
Chitosan derivatives etc..
High activity antioxidant autoxidation inactivation is fast, short life, Suitable ranges are narrow, can be increased after immobilization process
Strong its stability, retains higher enzymatic activity, with higher Acclimation temperature ability and broader pH accommodations.Due to enzyme
High activity, the additive capacity added into filter tip or pipe tobacco is less, and the original fragrance taste of flue gas cigarette after filter tip does not have
It is changed, the suction impression of consumer, easily recognized by consumers and acceptance will not be destroyed；Meanwhile, this modified filter tip can not
Composite filter tip need to be made, is put into production and is not needed huge scrap build to invest.
The shortcoming of prior art in view of the above, it is an object of the invention to provide a kind of immobilized catalase
Cigarette additive and its preparation method and application, for solving the problems of the prior art.
First aspect present invention provides a kind of immobilized catalase cigarette additive, the immobilized peroxidating
In hydrogen enzyme cigarette additive, immobilized reagent is selected from one or more in porous-starch, shitosan or chitosan derivatives etc.
Combination.
Second aspect present invention provides the preparation method of the immobilized catalase cigarette additive, the fixation
In the catalase cigarette additive of change, immobilized reagent is porous-starch, and the preparation method comprises the steps：
1) porous-starch is added in Catalase solution, refrigerator cold-storage is put into after being sufficiently stirred under room temperature overnight；
2) by step 1 products therefrom separation of solid and liquid, suction filtration, drying after gained precipitation washing, grinding is sieved and obtains final product immobilization
Catalase cigarette additive.
Preferably, in the step 1, the rate of charge of porous-starch and Catalase solution is 1g：8-12ml.
It is furthermore preferred that in the step 1, after porous-starch is added in Catalase solution, adjusting enzyme liquid cumulative volume
To 20ml.
Preferably, in the step 1, the concentration of enzymatic activity of the Catalase solution is 1*104CIU/ml, solvent is
Neutral phosphate buffer, concentration is 0.05mol/L.
Preferably, in the step 1, the time of stirring is 6-10h.
Preferably, in the step 1, refrigerator cold-storage temperature overnight is 0 DEG C.
Preferably, in the step 2, the concrete grammar by step 1 products therefrom separation of solid and liquid is：Step 1 gained is produced
Supernatant is abandoned after thing centrifugation.
Preferably, in the step 2, the concrete grammar of gained precipitation washing is：Deionized water is rinsed repeatedly.
Preferably, in the step 2, dry temperature is 35-45 DEG C.
Preferably, in the step 2, the actual conditions for sieving was 80 mesh sieves.
Third aspect present invention provides the preparation method of the immobilized catalase cigarette additive, the fixation
In the catalase cigarette additive of change, immobilized reagent is shitosan or chitosan derivatives, and the preparation method includes
Following steps：
1) first shitosan or chitosan derivatives be dissolved in glacial acetic acid aqueous solution, then NaOH water is added in resulting solution
Solution so as to produce white flock precipitate；
2) separation of solid and liquid, by gained precipitation neutrality is washed to, and chitin carrier is obtained final product after filtration；
3) gained chitin carrier is added in glutaraldehyde solution, is stood after being sufficiently stirred for, refiltered, wash；
4) step 3 products therefrom is added in phosphate buffer, adds Catalase solution, it is quiet after being sufficiently stirred for
Put, then suction filtration, drying, grinding is sieved and obtains final product immobilized catalase cigarette additive.
Preferably, in the step 1, the mean molecule quantity of shitosan is 2 × 105-5×105。
Preferably, in the step 1, the concentration of glacial acetic acid aqueous solution is 0.8-1.2wt%.
Preferably, in the step 1, the concentration of the NaOH aqueous solution is 1.5-2.5mol/L.
Preferably, in the step 1, shitosan or chitosan derivatives, glacial acetic acid aqueous solution, the NaOH aqueous solution feed intake
Than for 3g：1000-1200ml：4-6ml.
Preferably, in the step 3, glutaraldehyde solution is glutaraldehyde water solution, and concentration is 4-6wt%.
Preferably, in the step 3, chitin carrier, the rate of charge of glutaraldehyde solution are 1g：120-180ml.
Preferably, in the step 3, the temperature of stirring is room temperature, and the time span of standing is 6-8h.
In the step 3, the purpose of washing is to wash away unnecessary glutaraldehyde.
Preferably, in the step 4, the pH of phosphate buffer is 6.0-8.0, and concentration is 0.05mol/L.
Preferably, in the step 4, the activity of Catalase solution is 1*104CIU/ml, solvent delays for neutral phosphoric acid
Liquid is rushed, concentration is 0.05mol/L.
Preferably, in the step 4, step 3 products therefrom, phosphate buffer, the rate of charge of Catalase solution are
1g：40-50ml：14-18ml.
Preferably, in the step 4, the temperature of stirring is room temperature, and the time span of standing is 5-7h.
Preferably, in the step 4, dry temperature is 35-45 DEG C.
Preferably, in the step 4, the actual conditions for sieving was 80 mesh sieves.
In the preparation method of immobilized catalase cigarette additive provided by the present invention, shitosan is taken and is first handed over
The mode adsorbed after connection is fixed, i.e., shitosan it is first full cross-linked with crosslinking agent glutaraldehyde after adsorb catalase again, it is more existing
The method being crosslinked afterwards of first adsorbing have higher fixed rate.
Fourth aspect present invention provides the preparation method of described immobilized catalase cigarette additive in tobacco
The application of preparation field.
Fifth aspect present invention provides a kind of method for reducing gaseous phase free radical content in flue gas, will be described immobilized
Immobilized catalase cigarette additive prepared by the preparation method of catalase cigarette additive adds to acetic acid
Fibrous filter is uniformly sprayed in pipe tobacco.
Preferably, when adding to acetate fiber filter tip, the addition of the immobilized catalase cigarette additive
Between 0.2wt%-1.5wt% for acetate silk weight, optimum addition is 0.5wt%.
It is furthermore preferred that the immobilized catalase cigarette additive adds to the concrete grammar of acetate fiber filter tip
For：Add during tow slurrying, make modified filter tip.
It is furthermore preferred that the immobilized catalase cigarette additive adds to the concrete grammar of acetate fiber filter tip
For：Add during spin finishing to acetate fiber filter tip surface and make modified filter tip.
It is furthermore preferred that the immobilized catalase cigarette additive adds to the concrete grammar of acetate fiber filter tip
For：Additive is added in filter tip in the form of rupturable capsule, modified filter tip is made.
It is furthermore preferred that the immobilized catalase cigarette additive adds to the concrete grammar of acetate fiber filter tip
For：Additive is contained in filter tip in the form of a powder makes composite filter tip.
Preferably, when being uniformly sprayed to pipe tobacco, the addition of the immobilized catalase cigarette additive is cigarette
The 0.2wt%-1.5wt% of silk weight, optimum addition is 0.5wt%.
Immobilized catalase cigarette additive provided by the present invention is made into suction detection after feature filter tip,
Cigarette mainstream flue gas gaseous phase free radical content Jing after this filter tip is filtered declines 35%-43%, cigarette conventional index resistance to suction, nicotine,
Tar and CO are without significant change.The cigarette excitant of this feature filter tip of smokeing panel test slightly is reduced, and original smoke perfume is substantially not
Become.
In the present invention, catalase through environment-protecting asepsis fixing agent process, immobilization process after enzyme stability it is good,
The free radical in cigarette smoke can be effectively dispelled, spinnability, filament strength, elongation percentage of acetate fiber etc. are ensured while harm reduction
Shadow is not received.
Specific embodiment
Embodiments of the present invention are illustrated below by way of specific instantiation, those skilled in the art can be by this specification
Disclosed content understands easily other advantages and effect of the present invention.The present invention can also pass through concrete realities different in addition
The mode of applying is carried out or applies, the every details in this specification can also based on different viewpoints with application, without departing from
Various modifications and changes are carried out under the spirit of the present invention.
It should be clear that process equipment not concrete dated in the following example or device using conventional equipment in the art or
Device；All pressure values and scope are all referring to absolute pressure.
In addition, it is to be understood that one or more method and steps mentioned in the present invention do not repel before and after the combination step
Can also there is additive method step or additive method step can also be inserted between the step of these are specifically mentioned, unless separately
It is described；It should also be understood that the combination annexation between one or more the equipment/devices mentioned in the present invention is not repelled
Can also there is other equipment/device before and after the unit equipment/device or two equipment/devices specifically mentioning at these it
Between can also insert other equipment/device, unless otherwise stated.And, unless otherwise stated, the numbering of various method steps is only
Differentiate the convenient tool of various method steps, rather than to limit the ordering of various method steps or limiting enforceable model of the invention
Enclose, being altered or modified for its relativeness is of the invention enforceable when being also considered as in the case of without essence change technology contents
Category.
Embodiment 1
1g porous-starch is added in 10ml hydrogen peroxide enzyme liquids, concentration of enzymatic activity is 1*104CIU/ml, the appropriate phosphorus of supplement
Acid buffering solution (0.05mol/L, pH7.0) to enzyme liquid volume is 20ml.0 DEG C of refrigerator is put under room temperature after stirring 8h to refrigerate
At night, abandon supernatant after centrifugation, precipitate with deionized water rinse repeatedly after suction filtration, 40 DEG C of dryings, ground 80 mesh sieve obtains porous
The immobilized catalase cigarette additive of starch.
This cigarette additive is prepared into into feature acetate silk, addition tow weight by tow pulping process
1wt%, the spinnability of gained acetate silk, filament strength, elongation percentage are without substantially change.Tow is made into after filter tip
It is connected with NF cigarette and makes low free radical cigarette, with no added same model cigarette as control cigarette, smoking machine is taken out
Gaseous phase free radical content is determined after suction, the clearance rate for finding gaseous phase free radical is 36%.In addition, discovery of smokeing panel test, low free radical volume
Relative to NF cigarette, its fragrance taste is slightly reduced cigarette without substantially change, excitant.
Embodiment 2
Weigh 3g shitosans to be dissolved in the glacial acetic acid of 1L 1wt%, add 2mol/L NaOH aqueous solution 5ml so as to produce
White flock precipitate, takes out filter cake and is washed to neutrality, then chitin carrier is made in suction filtration dehydration.The 1g carriers are taken, is added
150ml 5wt% glutaraldehyde solutions, are stirred at room temperature, and stand 8h, wash away unnecessary glutaraldehyde, and suction filtration adds 50ml phosphoric acid
Cushioning liquid (0.05mol/L, pH7.0) and 16ml concentration of enzymatic activity are 1*104CIU/ml Catalase solutions, at room temperature
Stirring, stands suction filtration after 6h, 40 DEG C of dryings, and ground 80 mesh sieve obtains chitosan-immobilized catalase cigarette addition
Agent.
Add above-mentioned additive in the acetate silk of cigarette with dry method (powder addition), addition is 1.2mg, is added
Dosage is the 0.7wt% of tow weight, and spinnability, filament strength, the elongation percentage of gained acetate silk is without substantially change.
Gaseous phase free radical content is determined after smoking machine suction, the clearance rate for finding gaseous phase free radical is 39%.In addition, discovery of smokeing panel test, low
Free radical cigarette becomes softer relative to no added blank filter tip cigarette, flue gas, and fragrance taste is without substantially change.
Embodiment 3
1g porous-starch is added in 8ml hydrogen peroxide enzyme liquids, concentration of enzymatic activity is 1*104CIU/ml, the appropriate phosphorus of supplement
Acid buffering solution (0.05mol/L, pH7.0) to enzyme liquid volume is 20ml.0 DEG C of refrigerator is put under room temperature after stirring 10h to refrigerate
At night, abandon supernatant after centrifugation, precipitate with deionized water rinse repeatedly after suction filtration, 45 DEG C of dryings, ground 80 mesh sieve obtains porous
The immobilized catalase cigarette additive of starch.
This cigarette additive is added to acetate fiber filter tip by spin finishing process, addition tow weight
1.5wt%, spinnability, filament strength, the elongation percentage of gained acetate silk is without substantially change.Filter tip is connected system with cigarette
Into low free radical cigarette, with no added same model cigarette as control cigarette, gaseous phase free radical content is determined after smoking machine suction,
It was found that the clearance rate of gaseous phase free radical is 37%.In addition, discovery of smokeing panel test, low free radical cigarette relative to NF cigarette, its
Fragrance taste is slightly reduced without substantially change, excitant.
Embodiment 4
1g porous-starch is added in 12ml hydrogen peroxide enzyme liquids, concentration of enzymatic activity is 1*104CIU/ml, the appropriate phosphorus of supplement
Acid buffering solution (0.05mol/L, pH7.0) to enzyme liquid volume is 20ml.0 DEG C of refrigerator is put under room temperature after stirring 6h to refrigerate
At night, abandon supernatant after centrifugation, precipitate with deionized water rinse repeatedly after suction filtration, 35 DEG C of dryings, ground 80 mesh sieve obtains porous
The immobilized catalase cigarette additive of starch.
This cigarette additive is prepared into into feature acetate silk, addition tow weight by tow pulping process
0.2wt%, the spinnability of gained acetate silk, filament strength, elongation percentage are without substantially change.Tow is made into filter tip
It is connected with NF cigarette afterwards and makes low free radical cigarette, with no added same model cigarette as control cigarette, smoking machine
Gaseous phase free radical content is determined after suction, the clearance rate for finding gaseous phase free radical is 30%.In addition, discovery of smokeing panel test, low free radical
Cigarette becomes softer relative to no added blank filter tip cigarette, flue gas, and fragrance taste is without substantially change.
Embodiment 5
Weigh 3g shitosans to be dissolved in the glacial acetic acid of 1.2L 0.8wt%, add 1.5mol/L NaOH aqueous solution 6ml, make
It produces white flock precipitate, takes out filter cake and is washed to neutrality, then chitin carrier is made in suction filtration dehydration.The 1g carriers are taken,
180ml 4wt% glutaraldehyde solutions are added, is stirred at room temperature, stand 6h, wash away unnecessary glutaraldehyde, suction filtration adds 40ml
Phosphate buffer solution (0.05mol/L, pH7.0) and 14ml concentration of enzymatic activity are 1*104CIU/ml Catalase solutions, in room
The lower stirring of temperature, stands suction filtration after 7h, 45 DEG C of dryings, and ground 80 mesh sieve obtains chitosan-immobilized catalase cigarette with adding
Plus agent.
This cigarette additive is prepared into into feature acetate silk, addition tow weight by tow pulping process
0.5wt%, the spinnability of gained acetate silk, filament strength, elongation percentage are without substantially change.Tow is made into filter tip
It is connected with NF cigarette afterwards and makes low free radical cigarette, with no added same model cigarette as control cigarette, smoking machine
Gaseous phase free radical content is determined after suction, the clearance rate for finding gaseous phase free radical is 38%.In addition, discovery of smokeing panel test, low free radical
Cigarette becomes softer relative to no added blank filter tip cigarette, flue gas, and fragrance taste is without substantially change.
Embodiment 6
Weigh 3g shitosans to be dissolved in the glacial acetic acid of 1L 1.2wt%, add 2.5mol/L NaOH aqueous solution 4ml so as to
White flock precipitate is produced, filter cake is taken out and is washed to neutrality, then chitin carrier is made in suction filtration dehydration.The 1g carriers are taken, plus
Enter 120ml 6wt% glutaraldehyde solutions, be stirred at room temperature, stand 8h, wash away unnecessary glutaraldehyde, suction filtration adds 50ml phosphorus
Acid buffering solution (0.05mol/L, pH7.0) and 18ml concentration of enzymatic activity are 1*104CIU/ml Catalase solutions, in room temperature
Lower stirring, stands suction filtration after 5h, 35 DEG C of dryings, and ground 80 mesh sieve obtains chitosan-immobilized catalase cigarette addition
Agent.
This cigarette additive is uniformly sprayed in pipe tobacco, addition is the 0.5wt% of pipe tobacco weight, and pipe tobacco is made into low
Free radical filter tip cigarette, with no added same model filter tip cigarette as control cigarette, after smoking machine suction gaseous phase free radical is determined
Content, the clearance rate for finding gaseous phase free radical is 38%.In addition, discovery of smokeing panel test, low free radical cigarette is relative to no added sky
White filter tip cigarette, flue gas becomes softer, and fragrance taste is without substantially change.
In sum, the present invention effectively overcomes various shortcoming of the prior art and has high industrial utilization.
The principle and its effect of above-described embodiment only illustrative present invention, it is of the invention not for limiting.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and the scope without prejudice to the present invention to above-described embodiment.Cause
This, such as those of ordinary skill in the art is complete with institute under technological thought without departing from disclosed spirit
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (6)
1. a kind of immobilized catalase cigarette additive, in the immobilized catalase cigarette additive, Gu
Surely change reagent and be selected from shitosan, preparation method comprises the steps：
1) first shitosan is dissolved in glacial acetic acid aqueous solution, then the NaOH aqueous solution is added in resulting solution so as to produce white
Flocculent deposit；
2) separation of solid and liquid, by gained precipitation neutrality is washed to, and chitin carrier is obtained final product after filtration；
3) gained chitin carrier is added in glutaraldehyde solution, is stood after being sufficiently stirred for, refiltered, wash；
4) step 3 products therefrom is added in phosphate buffer, adds Catalase solution, stood after being sufficiently stirred for, then
Suction filtration, drying, grinding is sieved and obtains final product immobilized catalase cigarette additive.
2. the preparation method of immobilized catalase cigarette additive as claimed in claim 1, the immobilized mistake
In hydrogen oxide enzyme cigarette additive, immobilized reagent is shitosan, and the preparation method comprises the steps：
1) first shitosan is dissolved in glacial acetic acid aqueous solution, then the NaOH aqueous solution is added in resulting solution so as to produce white
Flocculent deposit；
2) separation of solid and liquid, by gained precipitation neutrality is washed to, and chitin carrier is obtained final product after filtration；
3) gained chitin carrier is added in glutaraldehyde solution, is stood after being sufficiently stirred for, refiltered, wash；
4) step 3 products therefrom is added in phosphate buffer, adds Catalase solution, stood after being sufficiently stirred for, then
Suction filtration, drying, grinding is sieved and obtains final product immobilized catalase cigarette additive.
3. the preparation method of immobilized catalase cigarette additive as claimed in claim 2, in the step 1, shell
Glycan, glacial acetic acid aqueous solution, the rate of charge of the NaOH aqueous solution are 3g：1000-1200ml：4-6ml.
4. the preparation method of immobilized catalase cigarette additive as claimed in claim 2, in the step 3, shell
Glycan carrier, the rate of charge of glutaraldehyde solution are 1g：120-180ml.
5. the preparation method of immobilized catalase cigarette additive as claimed in claim 2, in the step 4, walks
Rapid 3 products therefrom, phosphate buffer, the mass ratio that feeds intake of Catalase solution are 1g：40-50ml：14-18ml.
6. application of the immobilized catalase cigarette additive as claimed in claim 1 in field of tobacco preparation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510500332.0A CN105146745B (en) | 2013-10-18 | 2013-10-18 | Immobilized catalase additive for tobacco as well as preparation method and application of additive |
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| CN201510500332.0A CN105146745B (en) | 2013-10-18 | 2013-10-18 | Immobilized catalase additive for tobacco as well as preparation method and application of additive |
| CN201310493334.2A CN103535852B (en) | 2013-10-18 | 2013-10-18 | A kind of immobilized catalase cigarette additive and its preparation method and application |
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| CN201310493334.2A Active CN103535852B (en) | 2013-10-18 | 2013-10-18 | A kind of immobilized catalase cigarette additive and its preparation method and application |
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| CN104549085B (en) * | 2015-01-12 | 2016-06-08 | 中国科学院化学研究所 | A kind of antioxidase Nano capsule and its preparation method and application |
| CN105105335B (en) * | 2015-09-16 | 2019-01-11 | 安徽农业大学 | A kind of phosphate modified porous-starch quantitatively embeds tea polyphenol compound and its preparation method and application |
| CN105919154A (en) * | 2016-06-28 | 2016-09-07 | 云南瑞升烟草技术（集团）有限公司 | Blasting bead filter rod for reducing free radical in cigarette smoke |
| CN107048472A (en) * | 2017-05-26 | 2017-08-18 | 河南中烟工业有限责任公司 | Porous ceramics immobilization pectase and its application in reconstituted tobacoo process |
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| JPH0420286A (en) * | 1990-05-15 | 1992-01-23 | Mitsubishi Gas Chem Co Inc | Separation of enzyme |
| CN1836599A (en) * | 2006-04-19 | 2006-09-27 | 江南大学 | Preparation method of chitosan immobilized hydrogen peroxidase for cigarette filter tip additive |
| CN102199592B (en) * | 2011-04-02 | 2013-04-03 | 重庆大学 | Method for preparing mixed immobilized glucose oxidase/catalase microspheres |
| CN103013971A (en) * | 2012-12-12 | 2013-04-03 | 天津大学 | Immobilized enzyme and preparation method thereof |
| CN103283975A (en) * | 2013-03-27 | 2013-09-11 | 广州格拉姆生物科技有限公司 | A method of using spray drying for preparing probiotics micro-capsules |
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| CN105146745A (en) | 2015-12-16 |
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