CN105132495B - A kind of flavomycoin fermentation manufacturing technique - Google Patents
A kind of flavomycoin fermentation manufacturing technique Download PDFInfo
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- CN105132495B CN105132495B CN201510576266.5A CN201510576266A CN105132495B CN 105132495 B CN105132495 B CN 105132495B CN 201510576266 A CN201510576266 A CN 201510576266A CN 105132495 B CN105132495 B CN 105132495B
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Abstract
The present invention provides a kind of flavomycoin fermentation manufacturing technique, is to be obtained with the medium culture flavomycoin production bacterium containing cottonseed oil, the flavomycoin production bacterium is：Streptomyces bambergiensisYJ‑151。The flavomycoin fermentation manufacturing technique ferment effect of the present invention is good, and production cost is low, it can be seen from the figure that replacing soya-bean oil, fermentation level suitable with cottonseed oil.
Description
Technical field
The present invention relates to a kind of flavomycoin fermentation manufacturing techniques.
Background technology
In existing flavomycoin fermentation manufacturing technique, use soya-bean oil as the primary carbon source for producing bacterium, but soya-bean oil always
It is expensive, how to reduce production cost, and ferment effect can be obtained good flavomycoin is those skilled in the art institute
Technical problems to be solved.
Invention content
The present invention provides a kind of flavomycoin fermentation manufacturing techniques, and production cost is low, and ferment effect is good.
There is abundant cottonseed oil resource in Xinjiang, and price is cheaper than soya-bean oil, and the present invention substitutes soya-bean oil as life using cottonseed oil
The carbon source of bacterium is produced, fermentation level is suitable, and cost can be reduced effectively.
The present invention provides a kind of flavomycoin fermentation manufacturing technique, is produced with the medium culture flavomycoin containing cottonseed oil
What bacterium obtained, the flavomycoin production bacterium is：Streptomyces bambergiensisYJ-151。
Preferably, the culture medium is divided into seed culture medium and fermentation medium；Per in 100ml seed culture mediums
The content of cottonseed oil is 0.05-0.15g.
Preferably, the content of cottonseed oil is 1.0g-4.0 in every 100ml fermentation mediums.
Preferably, it is per the formula of 100ml seed culture mediums：Cornstarch 3.0g, corn steep liquor 0.5g, bean cake powder
2.0g, cottonseed oil 0.1g, calcium carbonate 0.3g, potassium dihydrogen phosphate 0.2g, alpha-amylase 0.01g/g dried starch, pure water surplus；
PH value 7.2~7.5.
Preferably, it is per the formula of 100ml fermentation mediums：Cornstarch 3.0g, corn steep liquor 0.5g, bean cake powder
2.5g, cottonseed oil 3.0g, dipotassium hydrogen phosphate 0.01g, calcium carbonate 0.3g, alpha-amylase 0.004g/g dried starch, ammonium sulfate 0.3g,
Magnesium sulfate 0.2g, cobalt chloride 0.0001g, ferrous sulfate 0.00003g, polyoxyethylene polyoxypropylene glycerin ether 0.05g, fatty alcohol
Polyoxyethylene ether O-25 0.3g, pure water surplus；PH6.9~7.1.
As further preferred embodiment, steps are as follows：
（1）Flavomycoin production bacterium strain is accessed in sterilized seed culture medium, 35-37 DEG C of cultivation temperature, shaking table turns
It fast 220-260 revs/min, cultivates 18-26 hours and obtains shake-flask seed culture solution；
（2）Above-mentioned shake-flask seed culture solution is transferred to sterilized seeding tank by sterile working, culture medium uses seed
Culture medium, speed of agitator 120-160r/min, 35-37 DEG C of culture 40-48h of tank temperature obtain seeding tank seed culture fluid；
（3）Above-mentioned seeding tank seed culture fluid is transferred to fermentation tank culture, culture medium uses fermentation medium, speed of agitator
80-130r/min；32 DEG C~37 DEG C of temperature, tank press 0.05 MPa, interior ventilation ratio 1 per minute：0.6-1:1.1；Fermentation
170-230 hours.
Preferably, step（1）Middle inoculum concentration is 0.45-0.55 milliliters of bacterium solution/50 milliliter culture mediums.
The flavomycoin fermentation manufacturing technique ferment effect of the present invention is good, and production cost is low, from figure 1 it appears that using cotton
Seed oil replaces soya-bean oil, fermentation level suitable.
Description of the drawings
Attached drawing is used to provide further understanding of the present invention, and a part for constitution instruction, the reality with the present invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings：
Fig. 1 be cottonseed oil and soya-bean oil respectively as culture medium raw material when the comparison of flavomycoin potency, wherein abscissa is small
When, ordinate is valence value（Units per ml）.
Specific implementation mode
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.
Bacterial strain and culture medium：
1. flavomycoin produces bacterium：The primary streptomycete of spotStreptomyces bambergiensisYJ-151：By Zhejiang money
River Bioisystech Co., Ltd presents；
2. culture medium：
2.1. seed culture medium（g/100ml）：Cornstarch：3.0, corn steep liquor 0.5, bean cake powder 2.0, cottonseed oil（It is edible
It is more than cottonseed oil three-level）0.05-0.15, calcium carbonate 0.3, potassium dihydrogen phosphate 0.2, alpha-amylase 0.01g/g dried starch, pH value
7.2~7.5.
2.2. fermentation medium（g/100ml）：Cornstarch 3.0, corn steep liquor 0.5, bean cake powder 2.5, cottonseed oil（It is edible
It is more than cottonseed oil three-level）1.0-4.0, dipotassium hydrogen phosphate 0.01, calcium carbonate 0.3, alpha-amylase 0.004g/g dried starch, ammonium sulfate
0.3, magnesium sulfate 0.2, cobalt chloride 0.0001, ferrous sulfate 0.00003, polyoxyethylene polyoxypropylene glycerin ether（Antifoaming agent）
0.05, fatty alcohol polyoxyethylene ether O-25（Emulsifier）0.3, pH6.9~7.1.
Steps are as follows for the flavomycoin fermentation manufacturing technique of the present invention：
（1）By the production strain of cryovial preservationStreptomyces bambergiensisYJ-151 strains access
In sterilized seed culture medium, inoculum concentration be 0.45-0.55 milliliters of bacterium solution/50 milliliter culture mediums, 35-37 DEG C of cultivation temperature,
220-260 revs/min of shaking speed cultivates 18-26 hours and obtains shake-flask seed culture solution.
（2）Above-mentioned shake-flask seed culture solution is transferred to sterilized seeding tank by sterile working, culture medium uses seed
Culture medium, speed of agitator 120-160r/ min, 35-37 DEG C of culture 40-48h of tank temperature obtain seeding tank seed culture fluid.
（3）Above-mentioned seeding tank seed culture fluid is transferred to fermentation tank culture, culture medium uses fermentation medium, speed of agitator
80-130r/ min；32 DEG C~37 DEG C of temperature, tank press 0.05 MPa, interior ventilation ratio (v/ per minute（v·min）) =1：
0.6-1:1.1。
（4）Per a flavomycoin potency is detected for 24 hours since 36h, ferments 170-230 hours, obtain flavomycoin.
（5）Conclusion：As a result referring to Fig. 1, from figure 1 it appears that replacing soya-bean oil, fermentation level suitable with cottonseed oil.
Embodiment 1
Steps are as follows for the flavomycoin fermentation manufacturing technique of the present invention：
（1）By the production strain of cryovial preservationStreptomyces bambergiensisYJ-151 strains access
In sterilized seed culture medium, inoculum concentration is 0.50 milliliter of bacterium solution/50 milliliter culture medium, 36 DEG C of cultivation temperature, shaking speed
It 240 revs/min, cultivates 22 hours and obtains shake-flask seed culture solution.
The formula of seed culture medium is：Cornstarch：3.0g, corn steep liquor 0.5g, bean cake powder 2.0g, cottonseed oil（Edible cotton
It is more than seed oil three-level）0.10g, calcium carbonate 0.3g, potassium dihydrogen phosphate 0.2g, alpha-amylase 0.01g/g dried starch, add water to
100ml, pH 7.3.
（2）Above-mentioned shake-flask seed culture solution is transferred to sterilized seeding tank by sterile working, culture medium uses seed
Culture medium, speed of agitator 140r/ min, 36 DEG C of culture 44h of tank temperature obtain seeding tank seed culture fluid.
（3）Above-mentioned seeding tank seed culture fluid is transferred to fermentation tank culture, culture medium uses fermentation medium, speed of agitator
110r/ min；34 DEG C of temperature, tank press 0.05 MPa, interior ventilation ratio (v/ per minute（v·min）) =1：0.8.
Fermentative medium formula is：Cornstarch 3.0g, corn steep liquor 0.5g, bean cake powder 2.5g, cottonseed oil（Edible cottonseed oil
It is more than three-level）3.0g, dipotassium hydrogen phosphate 0.01g, calcium carbonate 0.3g, alpha-amylase 0.004g/g dried starch, ammonium sulfate 0.3g, sulphur
Sour magnesium 0.2g, cobalt chloride 0.0001g, ferrous sulfate 0.00003g, polyoxyethylene polyoxypropylene glycerin ether（Antifoaming agent）0.05g、
Fatty alcohol polyoxyethylene ether O-25（Emulsifier）0.3g adds water to 100ml, pH 7.0.
（4）Fermentation 230 hours obtains flavomycoin, and the potency of flavomycoin is 9560 units per mls.
Embodiment 2
（1）By the production strain of cryovial preservationStreptomyces bambergiensisYJ-151 strains access
In sterilized seed culture medium, inoculum concentration is 0.45 milliliter of bacterium solution/50 milliliter culture medium, 37 DEG C of cultivation temperature, shaking speed
It 260 revs/min, cultivates 26 hours and obtains shake-flask seed culture solution.
The formula of seed culture medium is：Cornstarch：3.0g, corn steep liquor 0.5g, bean cake powder 2.0g, cottonseed oil（Edible cotton
It is more than seed oil three-level）0.15g, calcium carbonate 0.3g, potassium dihydrogen phosphate 0.2g, alpha-amylase 0.01g/g dried starch, add water to
100ml, pH 7.3.
（2）Above-mentioned shake-flask seed culture solution is transferred to sterilized seeding tank by sterile working, culture medium uses seed
Culture medium, speed of agitator 160r/min, 35 DEG C of culture 48h of tank temperature obtain seeding tank seed culture fluid.
（3）Above-mentioned seeding tank seed culture fluid is transferred to fermentation tank culture, culture medium uses fermentation medium, speed of agitator
130r/ min；32 DEG C of temperature, tank press 0.05 MPa, interior ventilation ratio (v/ per minute（v·min）) =1:1.1.
Fermentative medium formula is：Cornstarch 3.0g, corn steep liquor 0.5g, bean cake powder 2.5g, cottonseed oil（Edible cottonseed oil
It is more than three-level）1.0g, dipotassium hydrogen phosphate 0.01g, calcium carbonate 0.3g, alpha-amylase 0.004g/g dried starch, ammonium sulfate 0.3g, sulphur
Sour magnesium 0.2g, cobalt chloride 0.0001g, ferrous sulfate 0.00003g, polyoxyethylene polyoxypropylene glycerin ether（Antifoaming agent）0.05g、
Fatty alcohol polyoxyethylene ether O-25（Emulsifier）0.3g adds water to 100ml, pH 7.0.
（4）Fermentation 170 hours obtains flavomycoin, and the potency of flavomycoin is 7250 units per mls.
Embodiment 3
（1）By the production strain of cryovial preservationStreptomyces bambergiensisYJ-151 strains access
In sterilized seed culture medium, inoculum concentration is 0.55 milliliter of bacterium solution/50 milliliter culture medium, 35 DEG C of cultivation temperature, shaking speed
It 220 revs/min, cultivates 18 hours and obtains shake-flask seed culture solution.
The formula of seed culture medium is：Cornstarch：3.0g, corn steep liquor 0.5g, bean cake powder 2.0g, cottonseed oil（Edible cotton
It is more than seed oil three-level）0.05g, calcium carbonate 0.3g, potassium dihydrogen phosphate 0.2g, alpha-amylase 0.01g/g dried starch, add water to
100ml, pH 7.3.
（2）Above-mentioned shake-flask seed culture solution is transferred to sterilized seeding tank by sterile working, culture medium uses seed
Culture medium, speed of agitator 120r/min, 37 DEG C of culture 40h of tank temperature obtain seeding tank seed culture fluid.
（3）Above-mentioned seeding tank seed culture fluid is transferred to fermentation tank culture, culture medium uses fermentation medium, speed of agitator
80r/ min；37 DEG C of temperature, tank press 0.05 MPa, interior ventilation ratio (v/ per minute（v·min）) =1：0.6.
Fermentative medium formula is：Cornstarch 3.0g, corn steep liquor 0.5g, bean cake powder 2.5g, cottonseed oil（Edible cottonseed oil
It is more than three-level）4.0g, dipotassium hydrogen phosphate 0.01g, calcium carbonate 0.3g, alpha-amylase 0.004g/g dried starch, ammonium sulfate 0.3g, sulphur
Sour magnesium 0.2g, cobalt chloride 0.0001g, ferrous sulfate 0.00003g, polyoxyethylene polyoxypropylene glycerin ether（Antifoaming agent）0.05g、
Fatty alcohol polyoxyethylene ether O-25（Emulsifier）0.3g adds water to 100ml, pH 7.0.
（4）Fermentation 200 hours obtains flavomycoin, and the potency of flavomycoin is 8330 units per mls.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention's
Within protection domain.
Claims (2)
1. a kind of flavomycoin fermentation manufacturing technique, it is characterised in that：Steps are as follows：
（1）Flavomycoin production bacterium strain is accessed in sterilized seed culture medium, 35-37 DEG C of cultivation temperature, shaking speed
It 220-260 revs/min, cultivates 18-26 hours and obtains shake-flask seed culture solution；The flavomycoin produces bacterium：Streptomyces bambergiensis YJ-151；
（2）Above-mentioned shake-flask seed culture solution is transferred to sterilized seeding tank by sterile working, culture medium uses seed culture
Base, speed of agitator 120-160r/min, 35-37 DEG C of culture 40-48h of tank temperature obtain seeding tank seed culture fluid；
Include per 100ml seed culture mediums：Cornstarch：3.0g, 0.5 g of corn steep liquor, 2.0 g of bean cake powder, cottonseed oil 0.05-
0.15 g, 0.3 g of calcium carbonate, 0.2 g of potassium dihydrogen phosphate, alpha-amylase 0.01g/g dried starch, the pH value 7.2 of seed culture medium
~7.5；
（3）Above-mentioned seeding tank seed culture fluid is transferred to fermentation tank culture, culture medium uses fermentation medium, speed of agitator 80-
130r/min；32 DEG C~37 DEG C of temperature, tank press 0.05 MPa, interior ventilation ratio 1 per minute：0.6-1:1.1；Ferment 170-
230 hours；
Include per 100ml fermentation mediums：Cornstarch 3.0g, 0.5 g of corn steep liquor, 2.5 g of bean cake powder, cottonseed oil 1.0-
4.0 g, 0.01 g of dipotassium hydrogen phosphate, 0.3 g of calcium carbonate, alpha-amylase 0.004g/g dried starch, 0.3 g of ammonium sulfate, magnesium sulfate
0.2 g, 0.0001 g of cobalt chloride, 0.00003 g of ferrous sulfate, 0.05 g of polyoxyethylene polyoxypropylene glycerin ether, poly alkyl alcohol
0.3 g of ethylene oxide ether O-25, the pH value 6.9~7.1 of fermentation medium.
2. technique according to claim 1, it is characterised in that：Step（1）Middle inoculum concentration be 0.45-0.55 milliliters of bacterium solutions/
50 milliliters of culture mediums.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3992263A (en) * | 1974-07-23 | 1976-11-16 | Hoechst Aktiengesellschaft | Process for the preparation of moenomycin |
| CN102634559A (en) * | 2012-05-02 | 2012-08-15 | 潍坊祥维斯化学品有限公司 | Preparation method of flavomycin by fermentation |
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2015
- 2015-09-11 CN CN201510576266.5A patent/CN105132495B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3992263A (en) * | 1974-07-23 | 1976-11-16 | Hoechst Aktiengesellschaft | Process for the preparation of moenomycin |
| CN102634559A (en) * | 2012-05-02 | 2012-08-15 | 潍坊祥维斯化学品有限公司 | Preparation method of flavomycin by fermentation |
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Address after: 832000 No.3 Tianshan Road, No.94 community, Shihezi Development Zone, Urumqi, Xinjiang Uygur Autonomous Region Patentee after: Xinjiang Zheda sunshine Biotechnology Co., Ltd Address before: 832000 No.3 Tianshan Road, No.94 community, Shihezi Economic and Technological Development Zone, Xinjiang Uygur Autonomous Region Patentee before: Xinjiang Tianfu Yangguang Biological Science & Technology Co., Ltd. |
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Address after: 832000 No.3, Tianshan Road, No.94 community, Shihezi Development Zone, Urumqi City, Xinjiang Uygur Autonomous Region Patentee after: Xinjiang Shangni Biotechnology Co.,Ltd. Address before: 832000 No.3, Tianshan Road, No.94 community, Shihezi Development Zone, Urumqi City, Xinjiang Uygur Autonomous Region Patentee before: Xinjiang Zheda sunshine Biotechnology Co.,Ltd. |