CN105132166B - A kind of cigarette apple spice and preparation method thereof - Google Patents
A kind of cigarette apple spice and preparation method thereof Download PDFInfo
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- CN105132166B CN105132166B CN201510445524.6A CN201510445524A CN105132166B CN 105132166 B CN105132166 B CN 105132166B CN 201510445524 A CN201510445524 A CN 201510445524A CN 105132166 B CN105132166 B CN 105132166B
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- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 36
- 235000013599 spices Nutrition 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000000706 filtrate Substances 0.000 claims abstract description 52
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 19
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 claims abstract description 18
- 150000002148 esters Chemical class 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 230000008569 process Effects 0.000 claims abstract description 12
- 238000002156 mixing Methods 0.000 claims abstract description 11
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims abstract description 9
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 claims abstract description 9
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 claims abstract description 9
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims abstract description 9
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000011090 malic acid Nutrition 0.000 claims abstract description 9
- 239000001630 malic acid Substances 0.000 claims abstract description 9
- 229960001285 quercetin Drugs 0.000 claims abstract description 9
- 235000005875 quercetin Nutrition 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000000605 extraction Methods 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 80
- 239000004615 ingredient Substances 0.000 claims description 45
- 238000003756 stirring Methods 0.000 claims description 41
- 235000019441 ethanol Nutrition 0.000 claims description 32
- 238000005374 membrane filtration Methods 0.000 claims description 26
- 102000004190 Enzymes Human genes 0.000 claims description 25
- 108090000790 Enzymes Proteins 0.000 claims description 25
- 229940088598 enzyme Drugs 0.000 claims description 25
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 24
- 239000000919 ceramic Substances 0.000 claims description 24
- 238000002604 ultrasonography Methods 0.000 claims description 9
- HNAGHMKIPMKKBB-UHFFFAOYSA-N 1-benzylpyrrolidine-3-carboxamide Chemical compound C1C(C(=O)N)CCN1CC1=CC=CC=C1 HNAGHMKIPMKKBB-UHFFFAOYSA-N 0.000 claims description 8
- 239000004382 Amylase Substances 0.000 claims description 8
- 108010065511 Amylases Proteins 0.000 claims description 8
- 102000013142 Amylases Human genes 0.000 claims description 8
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 235000019418 amylase Nutrition 0.000 claims description 8
- OBNCKNCVKJNDBV-UHFFFAOYSA-N butanoic acid ethyl ester Natural products CCCC(=O)OCC OBNCKNCVKJNDBV-UHFFFAOYSA-N 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 8
- 230000008719 thickening Effects 0.000 claims description 8
- 229920001661 Chitosan Polymers 0.000 claims description 7
- 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 claims description 7
- 230000014759 maintenance of location Effects 0.000 claims description 7
- 108010059892 Cellulase Proteins 0.000 claims description 6
- 229940106157 cellulase Drugs 0.000 claims description 6
- PPXUHEORWJQRHJ-UHFFFAOYSA-N ethyl isovalerate Chemical compound CCOC(=O)CC(C)C PPXUHEORWJQRHJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 150000002772 monosaccharides Chemical class 0.000 abstract description 11
- 239000002304 perfume Substances 0.000 abstract description 10
- 150000004676 glycans Chemical class 0.000 abstract description 9
- 229920001282 polysaccharide Polymers 0.000 abstract description 9
- 239000005017 polysaccharide Substances 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 5
- 150000007524 organic acids Chemical class 0.000 abstract description 5
- 150000008442 polyphenolic compounds Chemical class 0.000 abstract description 5
- 235000013824 polyphenols Nutrition 0.000 abstract description 5
- 239000012528 membrane Substances 0.000 description 18
- 238000002137 ultrasound extraction Methods 0.000 description 13
- 239000003205 fragrance Substances 0.000 description 10
- 239000000463 material Substances 0.000 description 8
- 241000208125 Nicotiana Species 0.000 description 7
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 7
- ICMAFTSLXCXHRK-UHFFFAOYSA-N Ethyl pentanoate Chemical compound CCCCC(=O)OCC ICMAFTSLXCXHRK-UHFFFAOYSA-N 0.000 description 6
- 229910052573 porcelain Inorganic materials 0.000 description 6
- 238000009288 screen filtration Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 2
- GWYFCOCPABKNJV-UHFFFAOYSA-M 3-Methylbutanoic acid Natural products CC(C)CC([O-])=O GWYFCOCPABKNJV-UHFFFAOYSA-M 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 108010001682 Dextranase Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- GWYFCOCPABKNJV-UHFFFAOYSA-N beta-methyl-butyric acid Natural products CC(C)CC(O)=O GWYFCOCPABKNJV-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003546 flue gas Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
Abstract
The present invention provides a kind of preparation methods of cigarette apple spice, by the way that Quercetin and malic acid are added after crushing apple, extraction filtering thickness obtains filter residue and filtrate, then filtrate is concentrated, filter residue adds filter after water after enzymolysis solution is added, then concentration is refiltered, mixed with propylene glycol is added after component peace ratio mixing after filtrate and filter residue concentration uniformly to filter afterwards, it is eventually adding esters and obtains apple spice, cigarette prepared by the method remains the Ester of apple characteristic perfume with apple spice, be extracted to greatest extent has the organic acid of preferably effect in cigarette in apple, monosaccharide, polyphenols, and polysaccharide enzymolysis process is utilized, further increase the content of monosaccharide in apple spice.
Description
Technical field
The present invention relates to a kind of preparation method of tobacco aromaticss, a kind of specifically preparation side of cigarette apple spice
Method.
Background technology
Apple is a kind of fruit of china natural resources very abundant, and ripe apple is because containing Ester and with gracefulness
Comfortable fragrance has abundant cigarette flavor in cigarette, covers the effect of cigarette foreign gas.The monosaccharide that is rich in apple simultaneously,
Organic acid, polyphenols and Ester have in cigarette it is preferable increase flue gas concentration, abundant cigarette perfume, reduce stimulation and
Improve the effect of mouthfeel.A kind of " the apple extractum and preparation method thereof and in cigarette of Chinese Patent Application No. 200810073706.5
Application in flavoring " is directly by the broken extraction of apple, which, which obtains apple extractum, does not have the fragrance of apple script, and
Contents of monosaccharides is low.Chinese Patent Application No. 201310173732.6 " a kind of preparation method of cigarette apple extract " is to break apple
A series of extraction is carried out after broken, medicinal extract prepared by this method does not have apple fragrance.Chinese Patent Application No.
201110365359.5 " a kind of natural apple essences and preparation method thereof " are that fragrance is added after apple is squeezed the juice to allocate, should
Method is difficult to extract the Ester in apple, and the contents of monosaccharides in extracting solution is relatively low.
Invention content
The present invention is exactly to provide a kind of preparation side of cigarette apple spice according to deficiency present in above-mentioned technology of preparing
Method, the cigarette which prepares remain the Ester of apple characteristic perfume with apple spice, are extracted in apple to greatest extent
There is organic acid, monosaccharide, the polyphenols of preferably effect in cigarette, and polysaccharide enzymolysis process is utilized, further increase
The content of monosaccharide in apple spice.
Technical solution is used by the present invention solves above-mentioned technical problem：A kind of preparation side of cigarette apple spice is provided
Method includes the following steps：
(1) by apple it is broken after apple pulp is made, be then added apple pulp weight 0.1~0.2% Quercetin and 0.1~
0.2% malic acid is uniformly mixing to obtain mixing apple pulp；
(2) ethyl alcohol is added in the mixing apple pulp obtained to step (1) to ethyl alcohol mass percent concentration 80%~90%,
After stirring at normal temperature is uniform, extraction；
Wherein：The ingredient that can be worked in cigarette in apple is mainly some Esters, organic acid, Polyphenols object
Matter and monosaccharide, these substances can be dissolved in ethyl alcohol, contribute maximum Ester boiling point relatively low fragrance in these substances, insoluble
Yu Shui, therefore with the alcohol at normal temperature ultrasonic extraction apple pulp of concentration 80%-90%, most of fragrance object in apple can be extracted
Matter；
(3) first order filtrate and first order filter residue is obtained by filtration after extracting；
(4) first order filtrate obtained in step (3) is concentrated to give the first apple ingredient；
(5) water of 4~6 times of first order filter residue quality is added in the first order filter residue obtained into step (3), is heated to 90
It~100 DEG C, stirs, second level filtrate is obtained by filtration；Second level filtrate is cooled to 38~42 DEG C, and first order filter residue quality is added
After enzymolysis, ethyl alcohol is added to the mass percent concentration of ethyl alcohol 40~60% in 0.1~0.3% mixed enzyme solution enzymolysis,
Third level filtrate is obtained by filtration, after being stored 12~36 hours at a temperature of 5~10 DEG C, filters, be concentrated to give the second apple ingredient；
(6) by the first apple ingredient and the second apple ingredient according to mass ratio 1:1~1:6 ratio is mixed, and is obtained
Mixed liquor is added the propylene glycol of mixed liquor quality 20%~40%, is filtered after stirring evenly, obtain third apple ingredient；
Wherein：Heating stirring can promote the dissolving of water-soluble polysaccharide, while can improve the solubility of water-soluble polysaccharide.
So as to make the content of polysaccharide in solution higher, analysed even if low temperature has par-tial polysaccharide during digesting because temperature reduces
The polysaccharide for going out, but being precipitated still keeps the state to suspend in the solution, can still participate in enzymolysis, ensure that relatively large number of
Water-soluble polysaccharide is hydrolyzed into monosaccharide, improves the content of monosaccharide in enzymolysis liquid, and in addition high-temperature stirring can kill solution in 30 minutes
In microorganism, allowing in enzymolysis process does not have microbial reproduction, avoids because of the acid drop that the mass propagation of various microorganisms generates
The low pH value of solution reduces enzymolysis efficiency to affect the activity of enzyme；
(7) esters are added in the third apple ingredient obtained into step (6), it is fragrant that cigarette apple is obtained after stirring evenly
Material.
Further, in the step (4) thickening temperature be 45~60 DEG C, first order filtrate be concentrated into density 1.1 ±
0.05g/cm3。
Further, the filtering detailed process in the step (5) before enzymolysis is：It is carried out with the sieve of 20~60 mesh of aperture
Filtering, then with the inorganic ceramic membrane filtration in the apertures 90~110nm, obtains second level filtrate；Filter process after enzymolysis is：With
The sieve of 20~60 mesh of aperture is filtered, and is then used the inorganic ceramic membrane filtration in the aperture apertures 90~110nm, is obtained the third level
Filtrate, after 5~10 DEG C of storages 12~36 hours, with 0.7~0.9 μm of the membrane filtration in aperture, to utmostly retain apple
In fragrance.
Further, the filtering detailed process of the step (3) is：It is filtered with the sieve of 20~60 mesh of aperture, so
Afterwards with the inorganic ceramic membrane filtration in the apertures 90~110nm, first order filtrate is obtained.
Further, in the step (5), after obtained third level filtrate filtering, it is concentrated into 1.2 ± 0.05g/ of density
cm3。
Further, the enzymolysis process in the step (5) is：Mixed enzyme solution, ultrasound enzymolysis 2~4 hours, temperature is added
38 DEG C~42 DEG C；Wherein, ultrasound-assisted enzymolysis is carried out in enzymolysis process, can increase the combination probability of enzyme and polysaccharide, is improved
Enzymolysis efficiency.
Further, ethyl alcohol used in the step (2) and step (5) is the food-grade ethanol of mass concentration 95%.
Further, in the step (5) mixed enzyme solution be chitosan enzyme, cellulase, amylase, protease mixing
Enzyme solution.
Further, esters are to account for the isovaleric acid isoamyl of third apple ingredient quality 0.2~0.7% in the step (7)
Ester, 0.5~1.0% ethyl isovalerate, 0.1~0.3% ethyl acetate, 0.1~0.5% ethyl butyrate mixing, be added
Mixing esters make apple feature fruity more obvious, and fragrance is more plentiful true to nature.
A kind of cigarette apple spice, the cigarette apple spice are the preparation of cigarette apple spice described in above-mentioned any one
Cigarette apple spice prepared by method.
The invention has the characteristics that：Cigarette prepared by the present invention is with apple spice rich in abundant monosaccharide, organic acid, polyphenol
Substance and Ester have apparent apple fragrance；Esters are added to stir evenly, you can obtain natural cigarette apple perfume
Material so that apple feature fruity is more obvious, and fragrance is more plentiful true to nature.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.The specific embodiments described herein are merely illustrative of the present invention, is not used to limit this hair
It is bright.
Embodiment 1
(1) apple pulp will be made after selected transparent apple Mechanical Crushing, the Quercetin of apple weight 0.1% is then added
Malic acid with 0.2%, stirs evenly immediately；
(2) 95% food-grade ethanol is added in the apple pulp stirred evenly into step (1), makes the concentration of final ethyl alcohol
85%, stirring at normal temperature carries out ultrasonic extraction 30 minutes after 30 minutes for control；
(3) it is tentatively filtered with the sieve of 20 mesh of aperture after ultrasonic extraction, then uses the inorganic pottery in the apertures 100nm
Porcelain membrane filtration obtains clear first order filtrate, is then combined with the first order filter residue that screen filtration and ceramic membrane filter obtain；
(4) first order filtrate obtained in step (3) is concentrated in vacuo, 50 DEG C of thickening temperature, being concentrated into density is
1.15g/cm3, obtain the first apple ingredient；
(5) step (3) is merged to the pure water that 4 times of quality are added in obtained first order filter residue, is heated to 95 DEG C of stirrings
30 minutes.It is tentatively filtered with the sieve of 20 mesh of aperture while hot, then uses the inorganic ceramic membrane filtration in the apertures 100nm, obtain
Clear second level filtrate is cooled to 40 DEG C, and the shell that step (3) merges obtained first order filter residue quality 0.1-0.3% is added
The mixed enzyme solution of dextranase, cellulase, amylase, protease, ultrasound enzymolysis 4 hours, control temperature is constant at 40 DEG C.Enzymolysis
After, the food-grade ethanol of concentration 95% is added in filtrate makes the final concentration of ethyl alcohol control 50%, with the sieve of 20 mesh of aperture
Net is tentatively filtered, and the inorganic ceramic membrane filtration in the apertures 100nm is then used, and obtains clear third level filtrate, 5 DEG C of low temperature storages
It after depositing 24 hours, is filtered with 0.8 μm of the filter membrane in aperture, it is 1.25g/cm to be concentrated into density3, obtain the second apple ingredient；
(6) by the first apple ingredient and the second apple ingredient according to 1：4 ratio is mixed, and mixed liquor is obtained, and is added
The propylene glycol of mixed liquor mass fraction 20% is filtered with the filter membrane in 0.8 μm of aperture after stirring evenly, obtains third apple group
Point；
(7) natural iso-amyl iso-valeriate 0.2%, different is added in the natural third apple ingredient obtained in step 6
Ethyl valerate 0.5%, ethyl acetate 0.1%, ethyl butyrate 0.1% stir evenly and obtain final natural tobacco apple perfume
Material.
Embodiment 2
(1) apple pulp will be made after selected transparent apple Mechanical Crushing, the Quercetin of apple weight 0.2% is then added
Malic acid with 0.1%, stirs evenly immediately；
(2) 95% food-grade ethanol is added in the apple pulp stirred evenly into step (1), makes the concentration of final ethyl alcohol
90%, stirring at normal temperature carries out ultrasonic extraction 30 minutes after 30 minutes for control；
(3) it is tentatively filtered with the sieve of 40 mesh of aperture after ultrasonic extraction, then uses the inorganic pottery in the apertures 100nm
Porcelain membrane filtration obtains first order filtrate, is then combined with the first order filter residue that screen filtration and ceramic membrane filter obtain；
(4) first order filtrate obtained in step (3) is concentrated in vacuo, 55 DEG C of thickening temperature, being concentrated into density is
1.14g/cm3, obtain the first apple ingredient.(5) step (3) is merged and the pure of 6 times of quality is added in obtained first order filter residue
Water purification is heated to 90 DEG C and stirs 30 minutes.It is tentatively filtered with the sieve of 40 mesh of aperture while hot, then uses the apertures 100nm
Inorganic ceramic membrane filtration obtains second level filtrate, is cooled to 38 DEG C, and step (3) is added and merges obtained filter residue quality 0.2%
The mixed enzyme solution of chitosan enzyme, cellulase, amylase, protease, ultrasound enzymolysis 2-4 hours, control temperature is constant at 38 DEG C.
After enzymolysis, the food-grade ethanol of concentration 95% is added in filtrate makes the final concentration of ethyl alcohol control 50%, with 40 mesh of aperture
Sieve tentatively filtered, then use the apertures 100nm inorganic ceramic membrane filtration, obtain clear third level filtrate, 6 DEG C are low
It after temperature storage 24 hours, is filtered with 0.8 μm of the filter membrane in aperture, it is 1.23g/cm to be concentrated into density3, obtain the second apple group
Point；
(6) by the first apple ingredient and the second apple ingredient according to 1：3 ratio is mixed, and mixed liquor quality point is added
The propylene glycol of number 25% is filtered with the filter membrane in 0.8 μm of aperture after stirring evenly, obtains natural cigarette third apple group
Point；
(7) natural iso-amyl iso-valeriate 0.3%, different is added in the natural third apple ingredient obtained in step 6
Ethyl valerate 0.6%, ethyl acetate 0.2%, ethyl butyrate 0.2% stir evenly and obtain final natural tobacco apple perfume
Material.
Embodiment 3
(1) apple pulp will be made after selected transparent apple Mechanical Crushing, the Quercetin of apple weight 0.2% is then added
Malic acid with 0.1%, stirs evenly immediately；
(2) 95% food-grade ethanol is added in the apple pulp stirred evenly into step (1), makes the concentration of final ethyl alcohol
90%, stirring at normal temperature carries out ultrasonic extraction 30 minutes after 30 minutes for control；
(3) it is tentatively filtered with the sieve of 40 mesh of aperture after ultrasonic extraction, then uses the inorganic pottery in the apertures 100nm
Porcelain membrane filtration obtains clear first order filtrate, is then combined with the first order filter residue that screen filtration and ceramic membrane filter obtain；
(4) first order filtrate obtained in step (3) is concentrated in vacuo, 55 DEG C of thickening temperature, being concentrated into density is
1.1g/cm3, obtain the first apple ingredient；
(5) step (3) is merged to the pure water that 6 times of quality are added in obtained first order filter residue, is heated to 90 DEG C of stirrings
30 minutes.It is tentatively filtered with the sieve of 40 mesh of aperture while hot, then uses the inorganic ceramic membrane filtration in the apertures 100nm, obtain
Clear second level filtrate is cooled to 38 DEG C, and the chitosan that step (3) merges obtained first order filter residue quality 0.2% is added
The mixed enzyme solution of enzyme, cellulase, amylase, protease, ultrasound enzymolysis 3 hours, control temperature is constant at 38 DEG C.Enzymolysis finishes
Afterwards, the food-grade ethanol of concentration 95% is added in filtrate makes the final concentration of ethyl alcohol control 50%, with the sieve of 30 mesh of aperture into
The preliminary filtering of row, then uses the inorganic ceramic membrane filtration in the apertures 100nm, obtains clear third level filtrate, 6 DEG C of low-temperature storages 24
It after hour, is filtered with 0.8 μm of the filter membrane in aperture, it is 1.2g/cm to be concentrated into density3, obtain the second apple ingredient；
(6) by the first apple ingredient and the second apple ingredient according to 1：3 ratio carries out being mixed to get mixed liquor, is added mixed
The propylene glycol for closing liquid mass fraction 25% is filtered after stirring evenly with the filter membrane in 0.8 μm of aperture, obtains natural cigarette with the
Three apple ingredients；
(7) natural iso-amyl iso-valeriate 0.3%, different is added in the natural third apple ingredient obtained in step 6
Ethyl valerate 0.6%, ethyl acetate 0.2%, ethyl butyrate 0.2% stir evenly and obtain final natural tobacco apple perfume
Material.
Embodiment 4
(1) apple pulp will be made after selected transparent apple Mechanical Crushing, the Quercetin of apple weight 0.2% is then added
Malic acid with 0.1%, stirs evenly immediately；
(2) 95% food-grade ethanol is added in the apple pulp stirred evenly into step (1), makes the concentration of final ethyl alcohol
90%, stirring at normal temperature carries out ultrasonic extraction 30 minutes after 30 minutes for control；
(3) it is tentatively filtered with the sieve of 40 mesh of aperture after ultrasonic extraction, then uses the inorganic pottery in the apertures 100nm
Porcelain membrane filtration obtains clear first order filtrate, is then combined with the first order filter residue that screen filtration and ceramic membrane filter obtain；
(4) first order filtrate obtained in step (3) is concentrated in vacuo, 55 DEG C of thickening temperature, being concentrated into density is
1.08g/cm3, obtain the first apple ingredient；
(5) step (3) is merged to the pure water that 6 times of quality are added in obtained first order filter residue, is heated to 90 DEG C of stirrings
30 minutes.It is tentatively filtered with the sieve of 40 mesh of aperture while hot, then uses the inorganic ceramic membrane filtration in the apertures 100nm, obtain
Clear second level filtrate is cooled to 38 DEG C, and the chitosan that step (3) merges obtained first order filter residue quality 0.2% is added
The mixed enzyme solution of enzyme, cellulase, amylase, protease, ultrasound enzymolysis 2 hours, control temperature is constant at 38 DEG C.Enzymolysis finishes
Afterwards, the food-grade ethanol of concentration 95% is added in filtrate makes the final concentration of ethyl alcohol control 50%, with the sieve of 40 mesh of aperture into
The preliminary filtering of row, then uses the inorganic ceramic membrane filtration in the apertures 100nm, obtains clear third level filtrate, 6 DEG C of low-temperature storages 24
It after hour, is filtered with 0.8 μm of the filter membrane in aperture, it is 1.16g/cm to be concentrated into density3, obtain the second apple ingredient；
(6) by the first apple ingredient and the second apple ingredient according to 1：4 ratio is mixed, and mixed liquor quality point is added
The propylene glycol of number 25% is filtered with the filter membrane in 0.8 μm of aperture after stirring evenly, obtains natural cigarette third apple group
Point；
(7) natural iso-amyl iso-valeriate 0.3%, different is added in the natural third apple ingredient obtained in step 6
Ethyl valerate 0.6%, ethyl acetate 0.2%, ethyl butyrate 0.2% stir evenly and obtain final natural tobacco apple perfume
Material.
Embodiment 5
(1) apple pulp will be made after selected transparent apple Mechanical Crushing, the Quercetin of apple weight 0.2% is then added
Malic acid with 0.2%, stirs evenly immediately；
(2) 95% food-grade ethanol is added in the apple pulp stirred evenly into step (1), makes the concentration of final ethyl alcohol
85%, stirring at normal temperature carries out ultrasonic extraction 30 minutes after 30 minutes for control；
(3) it is tentatively filtered with the sieve of 40 mesh of aperture after ultrasonic extraction, then uses the inorganic pottery in the apertures 110nm
Porcelain membrane filtration obtains clear first order filtrate, is then combined with the first order filter residue that screen filtration and ceramic membrane filter obtain；
(4) first order filtrate obtained in step (3) is concentrated in vacuo, 50 DEG C of thickening temperature, being concentrated into density is
1.11g/cm3, obtain the first apple ingredient；
(5) step (3) is merged to the pure water that 6 times of quality are added in obtained filter residue, 90 DEG C is heated to and stirs 30 minutes,
It is tentatively filtered with the sieve of 40 mesh of aperture while hot, then uses the inorganic ceramic membrane filtration in the apertures 100nm, obtain clear the
Two level filtrate is cooled to 40 DEG C, and chitosan enzyme, cellulose that step (3) merges obtained first order filter residue quality 0.2% is added
The mixed enzyme solution of enzyme, amylase, protease, ultrasound enzymolysis 3 hours, control temperature is constant at 38 DEG C, after enzymolysis, in filtrate
The food-grade ethanol of concentration 95%, which is added, makes the final concentration of ethyl alcohol control 50%, and preliminary mistake is carried out with the sieve of 50 mesh of aperture
Then filter uses the inorganic ceramic membrane filtration in the apertures 100nm, obtains clear third level filtrate, 8 DEG C of low-temperature storages are after 24 hours,
It is filtered with 0.8 μm of the filter membrane in aperture, it is 1.16g/cm to be concentrated into density3, obtain the second apple ingredient；
(6) by the first apple ingredient and the second apple ingredient according to 1：4 ratio is mixed, and mixed liquor quality point is added
The propylene glycol of number 25% is filtered with the filter membrane in 0.8 μm of aperture after stirring evenly, obtains natural cigarette third apple group
Point；
(7) natural iso-amyl iso-valeriate 0.3%, different is added in the natural third apple ingredient obtained in step 6
Ethyl valerate 0.6%, ethyl acetate 0.2%, ethyl butyrate 0.2% stir evenly and obtain final natural tobacco apple perfume
Material.
Embodiment 6
(1) apple pulp will be made after selected transparent apple Mechanical Crushing, the Quercetin of apple weight 0.2% is then added
Malic acid with 0.2%, stirs evenly immediately；
(2) 95% food-grade ethanol is added in the apple pulp stirred evenly into step (1), makes the concentration of final ethyl alcohol
80%, stirring at normal temperature carries out ultrasonic extraction 30 minutes after 30 minutes for control；
(3) it is tentatively filtered with the sieve of 40 mesh of aperture after ultrasonic extraction, then uses the inorganic pottery in the apertures 100nm
Porcelain membrane filtration obtains clear first order filtrate, is then combined with the first order filter residue that screen filtration and ceramic membrane filter obtain；
(4) first order filtrate obtained in step (3) is subjected to cryogenic vacuum concentration, 50 DEG C of thickening temperature is concentrated into close
Degree is 1.13g/cm3, obtain the first apple ingredient；
(5) step (3) is merged to the pure water that 6 times of quality are added in obtained first order filter residue, is heated to 90 DEG C of stirrings
It 30 minutes, is tentatively filtered with the sieve of 40 mesh of aperture while hot, then uses the inorganic ceramic membrane filtration in the apertures 110nm, obtain
Clear second level filtrate is cooled to 40 DEG C, and chitosan enzyme, fiber that step (3) merges obtained filter residue quality 0.2% is added
The mixed enzyme solution of plain enzyme, amylase, protease, ultrasound enzymolysis 4 hours, control temperature is constant in 40 DEG C, after enzymolysis, filtrate
The middle food-grade ethanol that concentration 95% is added makes the final concentration of ethyl alcohol control 50%, and preliminary mistake is carried out with the sieve of 50 mesh of aperture
Then filter uses the inorganic ceramic membrane filtration in the apertures 100nm, obtains clear third level filtrate, 8 DEG C of low-temperature storages are after 24 hours,
It is filtered with 0.9 μm of the filter membrane in aperture, it is 1.15g/cm to be concentrated into density3, obtain the second apple ingredient；
(6) by the first apple ingredient and the second apple ingredient according to 1：5 ratio is mixed, and mixed liquor quality point is added
The propylene glycol of number 25% is filtered with the filter membrane in 0.9 μm of aperture after stirring evenly, obtains natural cigarette third apple group
Point；
(7) natural iso-amyl iso-valeriate 0.3%, different is added in the natural third apple ingredient obtained in step 6
Ethyl valerate 0.6%, ethyl acetate 0.2%, ethyl butyrate 0.2% stir evenly and obtain final natural tobacco apple perfume
Material.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Belong to those skilled in the art in the technical scope disclosed by the present invention, the change or replacement that can be readily occurred in all are answered
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be subject to the protection scope in claims.
Claims (2)
1. a kind of preparation method of cigarette apple spice, it is characterised in that：Include the following steps：
(1) by apple it is broken after apple pulp is made, be then added apple pulp weight 0.1~0.2% Quercetin and 0.1~
0.2% malic acid is uniformly mixing to obtain mixing apple pulp；
(2) ethyl alcohol is added in the mixing apple pulp obtained to step (1) to ethyl alcohol mass percent concentration 80%~90%, room temperature
After stirring evenly, extraction；
(3) first order filtrate and first order filter residue is obtained by filtration after extracting；The detailed process of filtering is：With aperture 20~60
Purpose sieve is filtered, and then with the inorganic ceramic membrane filtration in the apertures 90~110nm, obtains first order filtrate；
(4) first order filtrate obtained in step (3) is concentrated to give the first apple ingredient；Thickening temperature is 45~60 DEG C, the
Level-one filtrate is concentrated into 1.1 ± 0.05g/cm of density3；
(5) water of 4~6 times of first order filter residue quality is added in the first order filter residue obtained into step (3), it is heated to 90~
It 100 DEG C, stirs, second level filtrate is obtained by filtration；Second level filtrate is cooled to 38~42 DEG C, and first order filter residue quality 0.1 is added
After enzymolysis, ethyl alcohol is added to the mass percent concentration of ethyl alcohol 40~60%, mistake in~0.3% mixed enzyme solution enzymolysis
Filter obtains third level filtrate, after being stored 12~36 hours at a temperature of 5~10 DEG C, filters, is concentrated into 1.2 ± 0.05g/ of density
cm3Obtain the second apple ingredient；
Filtering detailed process before enzymolysis is：It is filtered with the sieve of 20~60 mesh of aperture, then with the apertures 90~110nm
Inorganic ceramic membrane filtration obtains second level filtrate；Filter process after enzymolysis is：It was carried out with the sieve of 20~60 mesh of aperture
Then the inorganic ceramic membrane filtration in the aperture apertures 90~110nm is used in filter, obtain third level filtrate, and 5~10 DEG C of storages 12~36 are small
Shi Hou, with 0.7~0.9 μm of the membrane filtration in aperture；
Enzymolysis process is：Mixed enzyme solution, ultrasound enzymolysis 2~4 hours, 38 DEG C~42 DEG C of temperature is added；Mixed enzyme solution is chitosan
The mixed enzyme solution of enzyme, cellulase, amylase, protease；
(6) by the first apple ingredient and the second apple ingredient according to mass ratio 1:1~1:6 ratio is mixed, and is mixed
Liquid is added the propylene glycol of mixed liquor quality 20%~40%, is filtered after stirring evenly, obtain third apple ingredient；
(7) esters are added in the third apple ingredient obtained into step (6), cigarette apple spice is obtained after stirring evenly；Ester
Class be account for 0.2~0.7% iso-amyl iso-valeriate of third apple ingredient quality, 0.5~1.0% ethyl isovalerate, 0.1~
0.3% ethyl acetate, 0.1~0.5% ethyl butyrate mixing.
2. a kind of cigarette apple spice, it is characterised in that：The cigarette with apple spice be claim 1 described in cigarette apple spice
Preparation method prepare cigarette apple spice.
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| CN106118886A (en) * | 2016-07-29 | 2016-11-16 | 湖北中烟工业有限责任公司 | A kind of cigarette Fructus Rosae Normalis spice and preparation method thereof |
| CN106954886A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of preparation method of cigarette apple extract |
| CN113907394B (en) * | 2021-09-28 | 2023-06-13 | 深圳市真味生物科技有限公司 | Preparation method of apple extract for cigarettes |
| CN114874855A (en) * | 2022-06-15 | 2022-08-09 | 河南省新郑金叶香料有限公司 | Preparation method of grape extract essence and application of grape extract essence in cigarettes |
| CN115624204A (en) * | 2022-11-07 | 2023-01-20 | 广西中烟工业有限责任公司 | Apple spice for cigarettes and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103087826A (en) * | 2011-11-08 | 2013-05-08 | 江西中烟工业有限责任公司 | Blueberry extract and preparation method thereof and application in cigarette |
| CN103232894A (en) * | 2013-05-10 | 2013-08-07 | 湖北中烟工业有限责任公司 | Cigarette apple extract preparation method |
| CN103484250A (en) * | 2013-08-16 | 2014-01-01 | 山东省食品发酵工业研究设计院 | Method for preparing red jujube essence and red jujube polysaccharides by using defective jujubes |
| CN103627523A (en) * | 2012-08-29 | 2014-03-12 | 江西中烟工业有限责任公司 | Sun-cured red tobacco leaf extract, preparation method therefor and applications thereof in cigarettes |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087826A (en) * | 2011-11-08 | 2013-05-08 | 江西中烟工业有限责任公司 | Blueberry extract and preparation method thereof and application in cigarette |
| CN103627523A (en) * | 2012-08-29 | 2014-03-12 | 江西中烟工业有限责任公司 | Sun-cured red tobacco leaf extract, preparation method therefor and applications thereof in cigarettes |
| CN103232894A (en) * | 2013-05-10 | 2013-08-07 | 湖北中烟工业有限责任公司 | Cigarette apple extract preparation method |
| CN103484250A (en) * | 2013-08-16 | 2014-01-01 | 山东省食品发酵工业研究设计院 | Method for preparing red jujube essence and red jujube polysaccharides by using defective jujubes |
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