CN105130941B - A kind of preparation method of grape seed extract - Google Patents
A kind of preparation method of grape seed extract Download PDFInfo
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- CN105130941B CN105130941B CN201510542574.6A CN201510542574A CN105130941B CN 105130941 B CN105130941 B CN 105130941B CN 201510542574 A CN201510542574 A CN 201510542574A CN 105130941 B CN105130941 B CN 105130941B
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- grape
- extract
- extraction
- grape pip
- powder
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- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 229940087603 grape seed extract Drugs 0.000 title claims abstract description 7
- 235000002532 grape seed extract Nutrition 0.000 title claims abstract description 7
- 239000001717 vitis vinifera seed extract Substances 0.000 title claims abstract description 7
- 238000000605 extraction Methods 0.000 claims abstract description 37
- 241000219095 Vitis Species 0.000 claims abstract description 34
- 235000009754 Vitis X bourquina Nutrition 0.000 claims abstract description 34
- 235000012333 Vitis X labruscana Nutrition 0.000 claims abstract description 34
- 235000014787 Vitis vinifera Nutrition 0.000 claims abstract description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000000284 extract Substances 0.000 claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 21
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 claims abstract description 18
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- 239000012141 concentrate Substances 0.000 claims abstract description 9
- 239000001294 propane Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000012530 fluid Substances 0.000 claims abstract description 7
- 238000007781 pre-processing Methods 0.000 claims abstract description 7
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 6
- 238000001179 sorption measurement Methods 0.000 claims abstract description 5
- 239000003480 eluent Substances 0.000 claims description 11
- 235000019441 ethanol Nutrition 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000006837 decompression Effects 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 7
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 abstract description 6
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 abstract description 6
- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 abstract description 6
- HGVVOUNEGQIPMS-UHFFFAOYSA-N procyanidin Chemical compound O1C2=CC(O)=CC(O)=C2C(O)C(O)C1(C=1C=C(O)C(O)=CC=1)OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 HGVVOUNEGQIPMS-UHFFFAOYSA-N 0.000 abstract description 6
- 229920002414 procyanidin Polymers 0.000 abstract description 6
- 238000001291 vacuum drying Methods 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 13
- 235000019198 oils Nutrition 0.000 description 13
- 229940023032 activated charcoal Drugs 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 239000008169 grapeseed oil Substances 0.000 description 2
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002019 anti-mutation Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 235000020985 whole grains Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention discloses a kind of preparation method of grape seed extract, comprises the following steps：1) grape pip is crushed, added water and yeast, at room temperature sealing and fermenting, obtain pre-processing grape pip powder after vacuum drying；2) fluid constituted is pressed as extractant using propane and dimethyl ether, subcritical abstraction, obtained extract as grape-kernel oil is carried out to pretreatment grape pip powder；After after grape-kernel oil extraction completely, it is changed to carry out subcritical abstraction by extractant of absolute ethyl alcohol, extract is concentrated under reduced pressure, after concentrate is dried through active carbon adsorption, obtained bronzing powder as procyanidin extract.The extracting method that the present invention is provided combines various effective extracting methods, can effectively improve the purity of yield and extract, so as to obtain the product of very high quality.
Description
Technical field
The present invention relates to the preparation method of plant extracts, specifically a kind of preparation method of grape seed extract.
Background technology
Grape pip is generally the leftover bits and pieces after grape wine brewing, can account for the 5%~7% of whole grain grape.Containing abundant in grape pip
Grape-kernel oil and OPC.Wherein, the unsaturated fatty acid content in grape-kernel oil is up to 90%, unrighted acid
More than 75% is linoleic acid, is a kind of high vegetable oil of quality.And OPC then has significant anti-oxidant and removed certainly
It is active by base, with the various functions such as angiocarpy, preventing hypertension, anti-mutation, antitumor, whitening and skin-protecting are protected, extensively
Applied to fields such as food, medicine and cosmetics.And the existing method that grape-kernel oil and OPC are extracted from grape pip
Have：Low yield, extract are there is in aqueous solution soaking method, organic solvent extractionprocess, supercritical extraction etc., these methods
The shortcoming that purity is low, production cost is high.
The content of the invention
The purpose of the present invention is in view of the shortcomings of the prior art, there is provided a kind of preparation method of grape seed extract, to have
The advantages of yield is high, product purity is high.
The present invention uses following technical scheme：
A kind of preparation method of grape seed extract, comprises the following steps：
1) grape pip is crushed, adds the water and 0.05%~0.15% yeast of grape pip weight 30%~40%, room
The lower sealing and fermenting of temperature 3~8 days, is dried under vacuum to moisture content≤6%, obtains pre-processing grape pip powder；
2) 2~3 are pressed with propane and dimethyl ether:4~7 weight is extractant than the fluid of composition, to pretreatment grape pip
Powder carries out subcritical abstraction, and Extracting temperature is 49~53 DEG C, and extractions pressure is 2.5~2.9MPa, extraction time for 30~
60min, obtained extract as grape-kernel oil；After after grape-kernel oil extraction completely, it is changed to enter by extractant of absolute ethyl alcohol
Row subcritical abstraction, extraction temperature is 58~63 DEG C, and extracting pressure is 3.4~3.7MPa, and extraction time is 30~50min, extraction
Thing is taken to be concentrated under reduced pressure into the 1/3~1/6 of original volume, after concentrate is dried through active carbon adsorption, obtained bronzing powder
As procyanidin extract.
In above-mentioned technical proposal,
The active carbon adsorption is molten with 70% ethanol for concentrate is crossed into activated-charcoal column with 0.5~1.0BV/h flow velocity
Eluent, is then concentrated and dried by liquid eluant, eluent.
It is preferred that, added water and yeast is respectively the 35% and 0.1% of grape pip weight.
It is preferred that, when extracting for the first time, the weight ratio of propane and dimethyl ether is 2.5:6, Extracting temperature is 52.5 DEG C, is extracted
Pressure is 2.7MPa, and extraction time is 40min.
Compared with prior art, the present invention has advantages below：
The extracting method that the present invention is provided combines various effective extracting methods, can effectively improve yield and extract
Purity, so as to obtain the product of very high quality.Wherein, obtained grape-kernel oil residual oil content is less than 1.5%；Obtained former cyanine
Plain yield may be up to 85%, and purity may be up to 98%.
Embodiment
It is further described below with specific embodiment, but the present invention is not limited to these embodiments.
Embodiment 1
1) grape pip is crushed, adds the water and 0.1% yeast of grape pip weight 35%, at room temperature sealing and fermenting 6 days,
Moisture content≤6% is dried under vacuum to, obtains pre-processing grape pip powder；
2) 2.5 are pressed with propane and dimethyl ether:6 weight is extractant than the fluid of composition, and pretreatment grape pip powder is entered
Row subcritical abstraction, Extracting temperature is 52.5 DEG C, and extraction pressure is 2.7MPa, and extraction time is 40min, and obtained extract is i.e.
For grape-kernel oil；After after grape-kernel oil extraction completely, it is changed to carry out subcritical abstraction, extraction temperature by extractant of absolute ethyl alcohol
For 60 DEG C, extracting pressure is 3.6MPa, and extraction time is 40min, and extract is concentrated under reduced pressure into the 1/5 of original volume, by concentrate
Activated-charcoal column is crossed with 0.7BV/h flow velocity, with 70% ethanol solution eluant, eluent, is then concentrated and dried eluent, what is obtained is red
Brown powder is procyanidin extract.
Embodiment 2
1) grape pip is crushed, adds the water and 0.1% yeast of grape pip weight 30%, at room temperature sealing and fermenting 8 days,
Moisture content≤6% is dried under vacuum to, obtains pre-processing grape pip powder；
2) 2 are pressed with propane and dimethyl ether:5 weight is extractant than the fluid of composition, and pretreatment grape pip powder is carried out
Subcritical abstraction, Extracting temperature is 53 DEG C, and extraction pressure is 2.5MPa, and extraction time is 50min, obtained extract as Portugal
Grape seed oil；After after grape-kernel oil extraction completely, it is changed to carry out subcritical abstraction by extractant of absolute ethyl alcohol, extraction temperature is 63
DEG C, extracting pressure is 3.4MPa, and extraction time is 40min, and extract is concentrated under reduced pressure into the 1/6 of original volume, by concentrate with
0.5BV/h flow velocity crosses activated-charcoal column, with 70% ethanol solution eluant, eluent, is then concentrated and dried eluent, what is obtained is reddish brown
Color powder is procyanidin extract.
Embodiment 3
1) grape pip is crushed, adds the water and 0.15% yeast of grape pip weight 38%, at room temperature sealing and fermenting 3
My god, moisture content≤6% is dried under vacuum to, obtains pre-processing grape pip powder；
2) 2.5 are pressed with propane and dimethyl ether:7 weight is extractant than the fluid of composition, and pretreatment grape pip powder is entered
Row subcritical abstraction, Extracting temperature is 49 DEG C, and extraction pressure is 2.6MPa, and extraction time is 60min, and obtained extract is
Grape-kernel oil；After after grape-kernel oil extraction completely, it is changed to carry out subcritical abstraction by extractant of absolute ethyl alcohol, extraction temperature is
58 DEG C, extracting pressure is 3.5MPa, and extraction time is 50min, and extract is concentrated under reduced pressure into the 1/3 of original volume, by concentrate with
0.5~1.0BV/h flow velocity crosses activated-charcoal column, with 70% ethanol solution eluant, eluent, is then concentrated and dried eluent, obtains
Bronzing powder is procyanidin extract.
Embodiment 4
1) grape pip is crushed, adds the water and 0.05% yeast of grape pip weight 40%, at room temperature sealing and fermenting 5
My god, moisture content≤6% is dried under vacuum to, obtains pre-processing grape pip powder；
2) 3 are pressed with propane and dimethyl ether:4 weight is extractant than the fluid of composition, and pretreatment grape pip powder is carried out
Subcritical abstraction, Extracting temperature is 51 DEG C, and extraction pressure is 2.9MPa, and extraction time is 30min, obtained extract as Portugal
Grape seed oil；After after grape-kernel oil extraction completely, it is changed to carry out subcritical abstraction by extractant of absolute ethyl alcohol, extraction temperature is 61
DEG C, extracting pressure is 3.7MPa, and extraction time is 30min, and extract is concentrated under reduced pressure into the 1/5 of original volume, by concentrate with
1.0BV/h flow velocity crosses activated-charcoal column, with 70% ethanol solution eluant, eluent, is then concentrated and dried eluent, what is obtained is reddish brown
Color powder is procyanidin extract.
Claims (3)
1. a kind of preparation method of grape seed extract, it is characterised in that comprise the following steps：
1) grape pip is crushed, adds the water and 0.05%~0.15% yeast of grape pip weight 30%~40%, at room temperature
Sealing and fermenting 3~8 days, is dried under vacuum to moisture content≤6%, obtains pre-processing grape pip powder；
2) 2~3 are pressed with propane and dimethyl ether:4~7 weight is extractant than the fluid of composition, and pretreatment grape pip powder is entered
Row subcritical abstraction, Extracting temperature is 49~53 DEG C, and extraction pressure is 2.5~2.9MPa, and extraction time is 30~60min, is obtained
The extract arrived as grape-kernel oil；After after grape-kernel oil extraction completely, the progress by extractant of absolute ethyl alcohol is changed to subcritical
Extraction, extraction temperature is 58~63 DEG C, and extracting pressure is 3.4~3.7MPa, and extraction time is 30~50min, extract decompression
The 1/3~1/6 of original volume, after concentrate is dried through active carbon adsorption is concentrated into, obtained bronzing powder is former flower
Blue or green extract；
The active carbon adsorption is washed for concentrate is crossed into carbon column with 0.5~1.0BV/h flow velocity with 70% ethanol solution
Eluent, is then concentrated and dried by de- agent.
2. preparation method according to claim 1, it is characterised in that：Before fermentation, added water and yeast is respectively grape
The 35% of seed weight and 0.1%.
3. preparation method according to claim 1, it is characterised in that：When extracting for the first time, the weight of propane and dimethyl ether
Than for 2.5:6, Extracting temperature is 52.5 DEG C, and extraction pressure is 2.7MPa, and extraction time is 40min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510542574.6A CN105130941B (en) | 2015-08-31 | 2015-08-31 | A kind of preparation method of grape seed extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510542574.6A CN105130941B (en) | 2015-08-31 | 2015-08-31 | A kind of preparation method of grape seed extract |
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| CN105130941A CN105130941A (en) | 2015-12-09 |
| CN105130941B true CN105130941B (en) | 2017-07-28 |
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| CN201510542574.6A Expired - Fee Related CN105130941B (en) | 2015-08-31 | 2015-08-31 | A kind of preparation method of grape seed extract |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107137952B (en) * | 2017-03-20 | 2019-03-19 | 河南雍华生态农业科技有限公司 | A kind of subcritical method for implanting of grape pip anthocyanidin |
| CN107445699A (en) * | 2017-08-30 | 2017-12-08 | 太仓市林港农场专业合作社 | A kind of preparation method of vegetable cultivation special efficient slow release fertilizer |
| CN107417412A (en) * | 2017-08-30 | 2017-12-01 | 太仓市林港农场专业合作社 | A kind of vegetable cultivation special efficient is sustained selenium-enriched fertilizer |
| CN107522545A (en) * | 2017-08-30 | 2017-12-29 | 太仓市林港农场专业合作社 | A kind of preparation method of vegetable cultivation special efficient sustained release selenium-enriched fertilizer |
| CN107417411A (en) * | 2017-08-30 | 2017-12-01 | 太仓市林港农场专业合作社 | A kind of vegetable cultivation special efficient slow release fertilizer |
| CN107721966A (en) * | 2017-10-30 | 2018-02-23 | 潍坊友容实业有限公司 | A kind of Suaeda salsa anthocyanidin and biogenic salt combined extraction method |
| CN107653227A (en) * | 2017-11-13 | 2018-02-02 | 重庆斯德姆生物技术有限公司 | A kind of inducing culture and abductive approach for promoting Differentiation of Marrow Stromal Stem Cells Into Neurons |
| CN107828723A (en) * | 2017-11-13 | 2018-03-23 | 重庆斯德姆生物技术有限公司 | A kind of type I collagen culture medium and preparation method thereof |
| CN108251357A (en) * | 2017-12-26 | 2018-07-06 | 重庆斯德姆生物技术有限公司 | A kind of induction cell culture fluid and differentiation method of the skeletal muscle stem Cells to Chondrocyte Differentiation |
| CN109207548B (en) * | 2018-09-06 | 2020-10-30 | 山东省花生研究所 | Peanut coat oligomeric proanthocyanidin, and preparation method and application thereof |
| CN112843780A (en) * | 2021-01-05 | 2021-05-28 | 陕西科技大学 | Pigment extraction process of mulberry pomace |
| CN112852182A (en) * | 2021-01-05 | 2021-05-28 | 陕西科技大学 | Pigment extraction process of blackcurrant raisin skin residues |
| CN112707880A (en) * | 2021-01-05 | 2021-04-27 | 陕西科技大学 | Anthocyanin extraction process for dried lycium ruthenicum fruits |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH09221484A (en) * | 1996-02-14 | 1997-08-26 | Kikkoman Corp | Production of proanthocyanidin |
| CN101692999A (en) * | 2009-10-22 | 2010-04-14 | 浙江大学 | Mouth wash containing grape pip procyanidin extract and application |
| CN102851114A (en) * | 2012-09-21 | 2013-01-02 | 南京泽朗农业发展有限公司 | Method for extracting grape seed oil and proanthocyanidin |
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