CN105124133A - Method for preparing high-anti-fatigue-action soybean peptide determined by microcalorimetric method and anti-fatigue action evaluation experiment - Google Patents
Method for preparing high-anti-fatigue-action soybean peptide determined by microcalorimetric method and anti-fatigue action evaluation experiment Download PDFInfo
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- CN105124133A CN105124133A CN201510600371.8A CN201510600371A CN105124133A CN 105124133 A CN105124133 A CN 105124133A CN 201510600371 A CN201510600371 A CN 201510600371A CN 105124133 A CN105124133 A CN 105124133A
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Abstract
The invention discloses a method for preparing high-anti-fatigue-action soybean peptide determined by a microcalorimetric method and an anti-fatigue action evaluation experiment, and belongs to the technical field of soybean deep processing. The optimal protease for preparing the high-anti-fatigue-action soybean peptide is alkaline protease by adopting the microcalorimetric method according to the Michaelis constant comparison of an enzymatic hydrolysis reaction, and a product anti-fatigue-action evaluation experiment is combined to obtain the method for preparing the high-anti-fatigue-action soybean peptide. The method can be used for effectively improving the anti-fatigue function of a product. Compared with an anti-fatigue soybean peptide produced by a conventional process, high-anti-fatigue-action soybean peptide has the advantages of remarkably improving the exercise tolerance of a body, remarkably reducing accumulation of blood lactic acid after body exercise, remarkably increasing the reserve level of hepatic glycogen and remarkably improving the anti-fatigue capability of a body. The high-anti-fatigue-action soybean peptide has the advantages of no pollution, low cost, simple process, remarkable anti-fatigue function, good water solubility, safety, no toxicity, no side effect and the like, has a relatively high popularization value, can be used for generating relatively high economic benefit and social benefit, and has a broad prospect.
Description
Technical field
The invention belongs to soybean deep processing technology field, especially a kind of microcalorimetric method is in conjunction with the preparation method of anti-fatigue active evaluation experimental determination high anti-fatigue activity Soybean Peptide.
Background technology
Current, anti-fatigue health-product containing, the functional food of China are still in the starting stage, and the theoretical foundation of most research and development of products comes from traditional traditional Chinese medicine and culture of health preserving.China's anti-fatigue health-product containing, functional food all have much room for improvement in the local flavor adaptability, product type etc. of the raising of the separation and extraction of efficacy study, functional components, absorptivity, product; Be applicable to sportsman, high strength requirement physical labourer, the requirement of special post worker occupation antifatigue, be applicable to the daily antifatigue demand of ordinary consumer, the popular antifatigue food of popular welcome can be obtained, also few in the market.Therefore, the research field of China's antifatigue food still has wide space, and potentiality to be exploited is huge.Anti-fatigue active peptide is as small molecule material in recent years, low with its molecular weight, absorptivity is high, activity is strong, consumption is few, the unique advantage of green health, causes the attention of people more and more.
Large quantity research proves to have anti-fatigue active peptide components in the product that soybean protein is formed through protease hydrolytic, and the Soybean Peptide of production both at home and abroad is at present the multiple peptide molecule mixture that soybean protein obtains through protease hydrolytic.The method Chinese scholars being prepared by protease hydrolytic to Soybean Peptide has carried out large quantifier elimination, be mostly the character by measuring product, determine the condition such as optimal reaction temperature, acidity, test method is more complicated, randomness is comparatively large, can not the optimised process of accurate quantitative analysis determination protease hydrolytic reaction.Cause protease hydrolytic technology controlling and process to be short of theoretical direction, it is difficult to control.Microcalorimetric method directly measures the thermal change of trace in enzyme-catalyzed reaction by highly sensitive micro-calorimeter, obtain protease to the thermal power-time graph of soybean protein catalytic reaction, quantitatively determine protease hydrolytic soybean protein isolate optimal reaction temperature, pH, there is highly sensitive, simple operation and other advantages.Microcalorimetric method quantitatively can determine the preparation method of high anti-fatigue activity Soybean Peptide more accurately in conjunction with anti-fatigue active evaluation experimental.
Summary of the invention
The object of the invention is to, overcome the deficiencies in the prior art, a kind of method preparing high anti-fatigue activity Soybean Peptide is provided.A kind of new research method microcalorimetric method of the present invention, produces the suitableeest protease of Soybean Peptide to hydrolysate of soybean protein, optimum condition carries out determining quantifier elimination, determine a kind of preparation method of new high anti-fatigue activity Soybean Peptide in conjunction with anti-fatigue active evaluation experimental.
For solving the problems of the technologies described above, technical scheme of the present invention is: a kind of microcalorimetric method is in conjunction with the preparation method of anti-fatigue active evaluation experimental determination high anti-fatigue activity Soybean Peptide, and step is as follows;
(1) obtain solution:
Solution is 1.: accurately take soybean protein isolate sample, with the cushioning liquid of different pH value (pH3.3-pH10.0) respectively compound concentration be 5-10gL
-1soybean protein isolate solution.
Solution is 2.: compound concentration is 0.1-0.3gL
-1neutral proteinase (enzyme activity 5.3 × 10
4u/g) solution, trypsase (enzyme activity 5.1 × 10
4u/g) solution, bromelain (enzyme activity 6.0 × 10
4u/g) solution, alkali protease (enzyme activity 5.7 × 10
4u/g) solution.
(2) 90 DEG C of soybean protein isolate solution carry out water bath processing 10min;
(3) measure thermal power time graph: get solution 1. 2ml put into stainless steel ampoule.On the connecting rod of titration micro-calorimeter around thin plastic pipe, Guan Zhongsheng protein enzyme solution 2. 1ml, first preheating, when after temperature constant, laid by stirring system, rotating speed is 60-120rpm, with peristaltic pump, 2. protein enzyme solution is injected ampoule, then carry out the mensuration of thermal power-time graph;
(4) by thermal power-time graph, michaelis constant Km and maximal rate Vmax when parsing neutral proteinase, trypsase, papain, each temperature of alkali protease (306.15K-336.15K), pH value (pH6.13-pH9.22) according to thermokinetic theory and Reduced extent method, and the relational expression set up between michaelis constant Km and temperature, pH value, thus obtain neutral proteinase optimum temperature 319.19K ﹑ optimal pH 7.13; Trypsase optimum temperature 323.27K ﹑ optimal pH 7.77; Bromelain optimum temperature 314.63K ﹑ optimal pH 6.99; Alkali protease optimum temperature 329.5K ﹑ optimal pH 8.25.
(5) by thermal power-time graph, the michaelis constant Km=24.02gL of neutral proteinase at its most thermophilic Du ﹑ optimal pH is tried to achieve
-1, trypsase is at the michaelis constant Km=42.41gL of its most thermophilic Du ﹑ optimal pH
-1, bromelain is at the michaelis constant Km=50.31gL of its most thermophilic Du ﹑ optimal pH
-1, alkali protease is at the michaelis constant Km=16.01gL of its most thermophilic Du ﹑ optimal pH
-1, thus can know that the affinity of basic protein enzyme-to-substrate soybean protein isolate is the strongest, determine that alkali protease is the suitableeest protease of catalyzing hydrolysis soybean protein isolate further in conjunction with anti-fatigue active evaluation experimental.
(6) the Soybean Peptide product obtained under different tests condition (combination of ratio at the bottom of concentration of substrate, enzyme, pH, temperature, enzyme) carries out anti-fatigue active evaluation experimental, the preparation method according to result of the test determination high anti-fatigue activity Soybean Peptide: enzyme reaction → separation and Extraction of sizing mixing → add → remove peculiar smell → remove inorganic salts → sterilising and enzyme inactivating → concentrated, spraying dry → high anti-fatigue activity Soybean Peptide product.Wherein add at the bottom of enzyme reaction substrate concentration 5%-10%, pH value 8.25, enzyme than 1-3%, hydrolysis temperature 329.5K, add the bromelain with alkali protease 1:2 after hydrolysis by novo soybean protein 3h, under 314.63K, pH6.99, be hydrolyzed the Soybean Peptide that 1h obtains high anti-fatigue activity.
The invention has the beneficial effects as follows: the microcalorimetric method that the present invention uses has highly sensitive, the advantages such as simple to operate, experimental result is accurate; The invention provides a kind of method preparing high anti-fatigue activity Soybean Peptide, effectively can improve the anti-fatigue effect of product, prove through anti-fatigue active evaluation experimental, tested group of mice burden swimming time significant prolongation, after swimming exercise, Serum lactic acid content significantly reduces, the level of reserve of the hepatic glycogen content after swimming exercise and Mouse Liver glycogen significantly improves, and is the antifatigue Soybean Peptide product of high-quality.The present invention is pollution-free, and cost is low, and technique is simple, product yield and product anti-fatigue effect high, good water solubility, safe, nontoxic, have no side effect, there is higher promotional value, may be used for the exploitation of health products, functional food, food additives.Protease hydrolytic technology controlling and process in simultaneously can producing soybean further process enterprise Soybean Peptide well provides theoretical direction, and produce higher economic worth and social benefit, its prospect is boundless.
Accompanying drawing explanation
Fig. 1 at T=315.15K, the thermal power-time graph of trypsase catalytic reaction during different acidity
Fig. 2 at pH=7.29, the thermal power-time graph of trypsase catalytic reaction during different temperatures
Detailed description of the invention:
The present invention will be described below to enumerate specific embodiment.It is to be noted that embodiment is only for the invention will be further described, do not represent invention protection domain, other people point out the nonessential amendment and adjustment made according to the present invention, still belong to scope.
Embodiment 1
1. the preparation of solution:
Solution is 1.: accurately take soybean protein isolate sample, with pH value be 6.70,7.21,7.73,8.21,8.73 cushioning liquid respectively compound concentration be 10gL
-1soybean protein isolate solution.
Solution is 2.: compound concentration is 0.2gL
-1trypsin solution.
2.90 DEG C of soybean protein isolate solution carry out water bath processing 10min;
3. experiment is carried out in 4ml stainless steel ampulla (titration micro-calorimeter).Get soybean protein isolate solution 2ml and put into stainless steel ampoule.On the connecting rod of titration micro-calorimeter around thin plastic pipe, Guan Zhongsheng alkaline protease solution 1ml, first preheating, as temperature (311.15K; 316.15K; 321.15K; 326.15K; 331.15K) constant after, stirring system is laid, rotating speed is 120rpm, with peristaltic pump, trypsin solution is injected ampoule, then the mensuration of thermal power-time graph is carried out, recorder starts record, thinks that experiment terminates, stir and stop when recording pen draws the straight line parallel with baseline.Every individual system repeats measuring three times, averages.
Experiment records at T=315.15K, and during different acidity, the thermal power-time graph of trypsase catalytic reaction is as Fig. 1, resolves obtain T=315.15K, K time different acidity (6.70,7.21,7.73,8.21,8.73) according to Fig. 1
mand V
max:: PH=6.70, K
m=51.11 and V
max=0.0959; PH=7.21, K
m=49.50 and V
max=0.1066; PH=7.73, K
m=48.80 and V
max=0.1183; PH=8.21, K
m=49.13 and V
max=0.1134; PH=8.73, K
m=50.70 and V
max=0.0996.
According to the data of Km and Vmax, with computer fitting, Km-pH curvilinear equation can be obtained, namely
K
m＝172.0-31.71PH+2.040PH
2，R＝0.9985，
The optimal pH asking extreme value to obtain the reaction of trypsase catalysis soybean protein hydrolysis to this curvilinear equation is 7.77.
Experiment records at pH=7.29, and during different temperatures, the thermal power-time graph of trypsase catalytic reaction is as Fig. 2, resolves obtain pH=7.29 different temperatures (311.15K according to Fig. 2; 316.15K; 321.15K; 326.15K; Km and Vmax:T=311.15K 331.15K), Km=56.40 and Vmax=0.0941; T=316.15K, Km=48.53 and Vmax=0.1123; T=321.15K, Km=45.81 and Vmax=0.1166; T=326.15K, Km=47.71 and Vmax=0.1159; T=331.15K, Km=52.28 and Vmax=0.1016;
According to the data of Km and Vmax, with computer fitting, K can be obtained
m-T curvilinear equation, namely
K
m=0.084T
2– 54.31T+8797, R=0.9950, the optimum temperature asking extreme value to obtain the reaction of trypsase catalysis soybean protein hydrolysis is 323.27K.
Embodiment 2
1. size mixing
Protein isolate is put into the temperature control enzyme reactor tank with agitator, add water and size mixing, slurry concentration about 10%;
2. add enzyme reaction
After sizing mixing, by substrate protein heavy 1% add alkaline microbial protease, limit is enzyme-added, and limit is stirred, and adds alkali at any time, keep pH value ≈ 8.25, mixing speed=120r/min, hydrolysis temperature remains on about 329.5K, and enzymolysis adds the bromelain with alkali protease 1:2 after 3 hours, 1h is hydrolyzed at bromelain optimal reaction temperature 314.63K, optimum response pH6.99.
3. separation and Extraction
Go out the mixed liquor after enzymolysis ferment treatment, pH4.5, and make non-enzymolysis protein precipitating, mixed liquor pumps in seperator, carries out Separation of Solid and Liquid, and effluent is all acid-soluble Soybean Peptide.Solid formation is water insoluble ingredients, adds alkali neutralization, can be used as bean dregs dry feed after oven dry;
4. remove peculiar smell
Soybean Peptide is taste compound in low or soybean at enzymolysis degree---when isoflavones, saponin(e etc. are without separation and Extraction process, often there is unacceptable peculiar smell, for removing peculiar smell, Soybean Peptide liquid can be processed by activated-charcoal column, Soybean Peptide liquid and active carbon ratio be about 1:10 (namely by time Soybean Peptide flow quantity be 1/10 of activated carbon grain volume), cross column temperature ≈ 40 DEG C, after charcoal treatment, the Soybean Peptide liquid of transparent color and luster, substantially tasteless can be obtained;
5. remove inorganic salts
Inorganic salts are removed through anion-cation exchange resin.
6. sterilising and enzyme inactivating
Through de-taste, except the Soybean Peptide liquid after Ficus caricaL, high temperature sterilization goes out enzyme.
7. concentrated, spraying dry
Through the Soybean Peptide liquid of sterilising and enzyme inactivating, pump into the economic benefits and social benefits enrichment facility that steam pressure is 180KPa, vacuum is 90Kpa, temperature is 55 DEG C, concentrated concentration reaches ≈ 40%, in pump-in pressure formula spray drying tower, inlet temperature is 170 DEG C, outlet temperature 80 DEG C, products obtained therefrom is " high anti-fatigue activity Soybean Peptide ".
Embodiment 3
Anti-fatigue active evaluation experimental
Animal: mouse, healthy Kunming kind, weight (25 scholar 2) g, be SPF level (no-special pathogen), by Tai'an, Tai Bang company provides.
Tested products: embodiment 2 product " high anti-fatigue activity Soybean Peptide "
Animal grouping and administration:
Prerun 3d, raises and train observation to mouse between preliminary trial period, observes its adaptive condition to new environment, trains its fixed point to search for food; Quantity delivered every day of feeding is raised rate table according to throwing and is determined, and is adjusted accordingly according to the situation of ingesting.Throw something and feed and follow fixed point, regularly, quantitatively, determine the principle of matter.Eliminate physique and locomitivity and on average have the mouse of notable difference.After prerun, mouse is divided into 4 groups at random, often organizes 30: control group, basic, normal, high dosage group; Control group gavage 300mgkg
-1d
-1physiological saline, often kind of basic, normal, high dosage group of sample sets gavage 100,200,300mgkg respectively
-1d
-1soybean Peptide, continuous gavage 30d.Observe the situations such as the drinking-water of mouse, spirit, hair and stool and urine every day.
1. high anti-fatigue activity Soybean Peptide is on the impact of mice burden swimming time
When experiment is to 30d, every test dose group and control group get mouse 10.Right side of mice stern heavy burden body weight 6%, puts into the tank went swimming of water temperature (25 ± 1) DEG C, depth of water 40cm, and record mouse is from the swimming time entered when water to sports fatigue occurs.Mouse head does not have below upstream face 7s no longer to float to be judged to be fatigue.The record tired time.Control group and each dosage group are on the impact of mice burden swimming time in table 1, and result shows that experimental group all obviously extended than the control group mice burden swimming time, and height agent group has significant difference.
Table 1 high anti-fatigue activity Soybean Peptide is on the impact of mice burden swimming time
Table2effectsofsoybeanpeptideontheloadswimmingtimeofmice
Note: * represents p<0.05, * * represents p<0.01, compares with control group
2. high anti-fatigue activity Soybean Peptide is on the impact of Mouse Blood lactic acid content
Mouse last gavage 30min, every test dose group and control group are got 10 2% (body weight) of bearing a heavy burden afterwards and are stopped after the water went swimming 60min of temperature (25 ± 1) DEG C, take a blood sample after quiet 15min for subsequent use, pull out eyeball blood sampling, with blood lactase acid salt analysis-e/or determining Serum lactic acid content, the results are shown in Table 2.
Table 2 high anti-fatigue activity Soybean Peptide is on the impact of Mouse Blood lactic acid content
Table2Effectofsoybeanpeptideonbloodlacticacidinmice
Note: * represents p<0.05, * * represents p<0.01, compares with control group
Find out that each dosage group of this product all has significant difference (P < 0.05) by table 2, the accumulation of lactic acid when illustrating that this product significantly can reduce body movement, thus the sense that lessens fatigue.Can judge that this product can remarkable anti-fatigue effect thus.
3. high anti-fatigue activity Soybean Peptide is on the impact of Mouse Liver glycogen content
30min after mouse last gavage, every test dose group and control group get the water tank swimming 90min that 10 in temperature are (25 ± 1) DEG C, put to death immediately, get liver to blot with filter paper after physiological saline rinsing, accurately take liver 200mg, add 4ml trichloroacetic acid, often pipe homogenate 1min, pour homogenate into centrifuge tube, with the centrifugal 15min of 3000r/min, supernatant is transferred to another in vitro.In precipitation, add 4ml trichloroacetic acid again, homogenate 1min, the centrifugal 15min of 3000r/min, gets supernatant again, and the supernatant centrifugal with first time merges, and fully mixes, and measures its absorbance (A) value in 620nm place.Calculate the content of hepatic glycogen, the results are shown in Table 3.
Table 3 high anti-fatigue activity Soybean Peptide is on the impact of Mouse Liver glycogen content
Table3Effectsofsoybeanpeptideonthecontentofliverglycogeninmice
Note: * represents p<0.05, * * represents p<0.01, compares with control group
After this product each dosage group mouse swimming exercise, hepatic glycogen content is all apparently higher than control group.Visible product, by significantly increasing the level of reserve of hepatic glycogen, is reached for body and provides energy thus the effect of alleviating physical fatigue.
Claims (4)
1. microcalorimetric method is in conjunction with a preparation method for anti-fatigue active evaluation experimental determination high anti-fatigue activity Soybean Peptide, it is characterized in that: the suitableeest protease, optimal pH, the optimum temperature that adopt microcalorimetric method determination hydrolyzed soy protein isolate; In conjunction with the preparation method of anti-fatigue active evaluation experimental determination high anti-fatigue activity Soybean Peptide.
2. as claimed in claim 1 a kind of microcalorimetric method in conjunction with the preparation method of anti-fatigue active evaluation experimental determination high anti-fatigue activity Soybean Peptide, it is characterized in that, micro-calorimeter is utilized to measure the thermal power-time graph of neutral proteinase, trypsase, bromelain, alkali protease hydrolyzed soy protein isolate when different temperatures (306.15K-336.15K) respectively, calculate the michaelis constant of each enzyme digestion reaction and maximum reaction rate respectively, quantitatively obtain the optimum temperature of above-mentioned protease hydrolytic soybean protein isolate.
3. as claimed in claim 1 a kind of microcalorimetric method in conjunction with the preparation method of anti-fatigue active evaluation experimental determination high anti-fatigue activity Soybean Peptide, it is characterized in that, micro-calorimeter is also utilized to measure the thermal power-time graph of neutral proteinase, trypsase, papain, alkali protease hydrolyzed soy protein isolate when different pH (pH6.13-pH9.22), calculate the michaelis constant of each enzyme digestion reaction and maximum reaction rate respectively, quantitatively obtain the optimal pH of above-mentioned protease hydrolytic soybean protein isolate.
4. a kind of microcalorimetric method is in conjunction with the preparation method of anti-fatigue active evaluation experimental determination high anti-fatigue activity Soybean Peptide as claimed in claim 1, and it is characterized in that, the suitableeest described protease is alkali protease; Its hydrolyzed soy protein isolate optimal pH is 8.25; Optimum temperature is 329.5K; The preparation method of described high anti-fatigue activity Soybean Peptide is enzyme reaction → separation and Extraction of sizing mixing → add → remove peculiar smell → remove inorganic salts → sterilising and enzyme inactivating → concentrated, spraying dry → high anti-fatigue activity Soybean Peptide product.Wherein add at the bottom of enzyme reaction substrate concentration 5%-10%, pH value 8.25, enzyme than 1-3%, hydrolysis temperature 329.5K, add the bromelain with alkali protease 1:2 after hydrolysis by novo soybean protein 3h, under 314.63K, pH6.99, be hydrolyzed the Soybean Peptide that 1h obtains high anti-fatigue activity.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1489923A (en) * | 2003-09-16 | 2004-04-21 | 北京陶正生物工程技术有限公司 | Method for producing soya protein bioactive peptide |
| CN1500881A (en) * | 2002-11-12 | 2004-06-02 | 陆晓民 | Process for producing soybean protein peptide using soybean as raw material |
| CN1593646A (en) * | 2004-07-16 | 2005-03-16 | 吉林大学 | Soybean peptide, its preparation and application |
| CN101974591A (en) * | 2010-11-11 | 2011-02-16 | 湖北远成药业有限公司 | Enzymatic method for extracting soy peptide |
| CN103343154A (en) * | 2013-07-19 | 2013-10-09 | 东北农业大学 | Preparation of soybean anti-fatigue biological active peptide |
-
2015
- 2015-09-18 CN CN201510600371.8A patent/CN105124133A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1500881A (en) * | 2002-11-12 | 2004-06-02 | 陆晓民 | Process for producing soybean protein peptide using soybean as raw material |
| CN1489923A (en) * | 2003-09-16 | 2004-04-21 | 北京陶正生物工程技术有限公司 | Method for producing soya protein bioactive peptide |
| CN1593646A (en) * | 2004-07-16 | 2005-03-16 | 吉林大学 | Soybean peptide, its preparation and application |
| CN101974591A (en) * | 2010-11-11 | 2011-02-16 | 湖北远成药业有限公司 | Enzymatic method for extracting soy peptide |
| CN103343154A (en) * | 2013-07-19 | 2013-10-09 | 东北农业大学 | Preparation of soybean anti-fatigue biological active peptide |
Non-Patent Citations (1)
| Title |
|---|
| 李向阳等: "菠萝蛋白酶催化大豆蛋白水解反应的热动力学", 《高等学校化学学报》 * |
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Application publication date: 20151209 |