CN105112458B - A method of alleviating the negative effect that poor liquid flows back to kitchen waste ethanol fermentation - Google Patents
A method of alleviating the negative effect that poor liquid flows back to kitchen waste ethanol fermentation Download PDFInfo
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Abstract
The present invention provide it is a kind of alleviating poor liquid reflux to the method for the negative effect of kitchen waste ethanol fermentation, belong to biomass castoff application technology as the second resource field.This method is after bacterial strain activation and kitchen garbage pretreatment, kitchen garbage saccharified liquid is put into ferment tank, after fermentation, zymotic fluid is distilled, recycles ethyl alcohol, when concentration of alcohol is heated down to stopping when can not measuring in zymotic fluid, storage tank liquid replaces distilled water to be pre-processed for kitchen garbage, after poor liquid circulating and recovering to after 46 times with poor liquid, poor liquid is subjected to electrodialysis process, continues reuse later.Negative effect of the by-product for fermentation that alcohol fermentation can obviously reduce alcohol fermentation is carried out by the above method, fermentation time can be shortened, improves fermentation efficiency.
Description
Technical field
The present invention relates to biomass castoff application technology as the second resource field, a kind of poor liquid reflux of alleviation is particularly related to meal
The method of the negative effect of kitchen waste ethanol fermentation.
Background technology
As a kind of reproducible strategic resources, oneself enters in the industrial products of state key development ethyl alcohol, but ethyl alcohol is sent out
Ferment industry is the industry for having larger pollution to environment at present.Waste water caused by traditional fermentation process is mostly by processing
After be discharged into natural water, although discharging many indexs such as COD in water after treatment, oneself is up to standard, compared with Natural Water
Still there is larger gap.
The patent of invention that Chinese Patent Application No. is 200410066017.3 discloses a kind of purified liquor fermentation process of producing high concentration
The close circulation technology of alcohol, disadvantage is that：Alcohol grain direct reuse causes slurry pH value relatively low, need plus ammonium hydroxide into
Row is adjusted, and substantially increases production cost.The application for a patent for invention that Chinese Patent Application No. is 201010576401.3 discloses
A kind of method of anaeration in normal temperature water outlet recycling of water resource production alcohol, the technology are disadvantageous in that：Need a large amount of oxygen
Or the oxidants such as air carry out oxidation processes, it is time-consuming of high cost, while by the technical finesse, a large amount of CO will be generated2,
It is discharged into air, aggravates greenhouse effects, extreme influence is caused to amblent air temperature.
A kind of improve disclosed in the patent of invention that Chinese Patent Application No. is 201010576411.7 is discharged with anaeration in normal temperature
For the alcohol producing process of ingredient water, comprise the steps of:Raw material is carried out by regulation material-water ratio and the anaerobic effluent after deamination
Mixing adjusts addition α-amylase after pH and heats cooking and liquefaction, it is cooling after addition saccharification enzymatic conversion, then inoculation fermentation after cooling down, hair
It is distilled after ferment, before the slops obtained to distillation carries out Anaerobic Treatment, needs the precipitation for carrying out sulfate radical,
For slops after Anaerobic Treatment, anaeration in normal temperature is discharged pH value 8.0 or so, when as spice water, needs that sulfuric acid is added
PH value is reduced, additionally needing progress deamination processing could be as the ingredient water of next group alcoholic fermentation, the place of one side sulfate radical
Reason substantially increases production cost, and the presence of another aspect sulfate radical can cause to corrode to equipment, seriously affects equipment use
Service life, or even cause the damage of equipment.
Poor liquid circulating fermentation technology refer to saccharification after kitchen waste everfermentation, distillation be made ethyl alcohol after, by what is obtained
Remaining grain liquid replaces water for the kitchen garbage fermentation process of next batch.The circulating fermentation technology great advantage of draff liquid is
While reducing pollution, the abundant high protein, amino acid and various metals ion contained in poor liquid is made full use of, improves ethyl alcohol
Yield.At the same time it can also save technical process water, the method recycled shows superiority.Poor liquid circulating reflux exists
The yield of ethyl alcohol can be improved in kitchen garbage ethanol process, and poor liquid may be implemented and recycle, reduce fermentation at
This.
Although poor liquid reflux can largely reduce the generation of poor liquid, in its fermentation process that flows back
In, certain negative effect can be generated to alcohol fermentation, had research to be returned using flocculation precipitation pretreatment, the poor liquid in part at present
The methods of stream alleviates the negative effect of ethyl alcohol grain liquid reflux fermentation, but the complete poor liquid reflux of kitchen waste ethanol fermentation is ground
Study carefully less, the reflux fermentation comparison starchy material grain liquid reflux fermentation of kitchen garbage grain liquid is easier to cause the product of by-product lactic acid
Tired, since lactic acid is higher boiling acid, burning point is also higher, is not easy to remove during distilling ethyl alcohol, and lactic acid can be to kitchen rubbish
The reflux fermentation of rubbish grain liquid generates larger negative effect, therefore needs to carry out the negative effect that relevant scientific research solves reflux fermentation.
Invention content
The technical problem to be solved in the present invention is to provide a kind of poor liquid reflux of alleviation to the negative of kitchen waste ethanol fermentation
The method of influence, this method specifically include following steps：
(1) bacterial strain activates：Angel dry ferment is put into the conical flask equipped with glucose solution, glucose solution 2%
Glucose be added in deionized water and be made, the adding proportion of Angel dry ferment is dry according to 20 times of extension rate, i.e. 1 gram of Angel
Yeast is put into 20 grams of glucose solutions, is placed in 35 DEG C of shaking table and is cultivated 2-3h；
Kitchen garbage pre-processes：Kitchen waste is crossed grind mixing after, kitchen garbage and distilled water are pressed 2:1~1:1
Mass ratio mixes, and adds carbohydrase so that every gram of zymotic fluid 50-100 unit enzyme activity is stirred evenly, is placed in 60 DEG C of water-baths
Be saccharified 6h, takes supernatant for fermenting after centrifugation；
(2) supernatant in step (1) is put into fermentation tank, pH to 5-6 is adjusted, by the bacterial strain after activation according to rate of vaccination
Fermentation tank is added in 2%~4% (20 milliliters~40 milliliters of the yeast juice that i.e. 1000 grams of solution are added after activation), and control temperature exists
30-37 DEG C ferments, and fermentation time was controlled at 36-96 hours, after fermentation, 80 DEG C of distillation processing is carried out to zymotic fluid,
Recycle ethyl alcohol, when in zymotic fluid concentration of alcohol down to when can not measure stop heating, storage tank liquid；
(3) the poor liquid of gained in step (2) is substituted distilled water, repeated for kitchen garbage preprocessing process in step (1)
Step (1) and step (2)；
(4) after poor liquid circulating and recovering 4-6 times, ethanol production has dropped 15-20% than original using distilled water, when fermentation
Between by extending to 96h for 24 hours, at this point, carrying out electrodialysis process to the poor liquid of last time gained, electrodialysis is specially：Poor liquid is through 0.2
μm inorganic ceramic membrane filtration (can remove the impurity such as thalline and partially protein, pigment) after obtain permeate, permeate enters
Three Room bipolar membrane electrodialysis devices are recycled, and electrodialytic process constant pressure (23.0V) operation keeps temperature constant (30 in the process
DEG C), each room flow rate of liquid of device is 10L/h, and each room pH value is measured every 20min, and each room pH, breast is measured by sampling every 40min
The concentration of acid and amino acid, stops electrodialysis when lactic acid concn in permeate is down to 20-40g/L, obtains poor liquid after electrodialysis,
Poor liquid replaces distilled water to be used for step (1) again after electrodialysis.
The above-mentioned technical proposal of the present invention has the beneficial effect that：
This method carries out negative effect of the by-product for fermentation that alcohol fermentation can obviously reduce alcohol fermentation, can contract
Short fermentation time improves fermentation efficiency.
Description of the drawings
Fig. 1 is method and technology route of the poor liquid reflux of alleviation to the negative effect of kitchen waste ethanol fermentation of the present invention
Figure.
Specific implementation mode
To keep the technical problem to be solved in the present invention, technical solution and advantage clearer, below in conjunction with attached drawing and tool
Body embodiment is described in detail.
The present invention flows back the problems such as larger to alcohol fermentation negative effect for existing tank liquor circulating fermentation technology tank liquor,
A kind of method for alleviating poor liquid reflux to the negative effect of kitchen waste ethanol fermentation is provided.
As shown in Figure 1, for the Technology Roadmap of this method.After carrying out bacterial strain activation and kitchen garbage pretreatment, obtain
Supernatant (i.e. saccharified liquid) and saccharification slag, obtained supernatant is fermented, and obtains tunning, after tunning distillation,
Recycle ethyl alcohol, remaining for poor liquid, poor liquid reflux continues on for kitchen garbage pretreatment, after poor liquid circulating and recovering 4-6 times, into
Row electrodialysis removes lactic acid therein etc., then proceedes to reuse.
It is illustrated with reference to specific embodiment.
Embodiment 1
(1) bacterial strain activates：Angel dry ferment is put into (i.e. Portugal in the conical flask of the deionized water containing 2% glucose
Grape sugar juice), it is placed in 35 DEG C of shaking table and cultivates 2h.
Kitchen garbage pre-processes：Kitchen waste is crossed grind mixing after, according to kitchen garbage and water 2:1 (mass ratio) mixes
(first is water, and the poor liquid with fermentation ends is needed to replace water circulate operation after distillation later), adds carbohydrase and makes every gram
Zymotic fluid is 50 enzyme activity, i.e. 50U/g is stirred evenly, and is placed in 60 DEG C of water-baths the 6h that is saccharified, and takes supernatant for sending out after centrifugation
Ferment.
(2) kitchen garbage supernatant (i.e. saccharified liquid) is put into fermentation tank, adjusts pH to 5, then (i.e. by the bacterial strain after activation
Yeast) fermentation tank is added (according to inoculation 2%), control temperature is fermented at 34 DEG C, and fermentation time was controlled at 60 hours, was obtained
The yield of ethyl alcohol is 44g/L, and ferments and completed in left and right for 24 hours.
After fermentation, 80 DEG C of distillations processing is carried out to zymotic fluid, recycles ethyl alcohol, when in zymotic fluid concentration of alcohol down to not
Stop heating, storage tank liquid when can measure.
(3) distilled water is replaced to repeat step (1) and step (2) the poor liquid of gained in (2)；
(4) it repeats the above process five times, the yield for obtaining ethyl alcohol is 38g/L, and fermentation time terminates in 96h or so.Than
It is greatly prolonged, was extended to from 24 hours 96 hours, concentration of alcohol is reduced to 38g/L from 44g/L using the time of distilled water.
Comparative example
(1) bacterial strain activates：Angel dry ferment is put into (i.e. Portugal in the conical flask of the deionized water containing 2% glucose
Grape sugar juice), it is placed in 35 DEG C of shaking table and cultivates 2h.
Kitchen garbage pre-processes：Kitchen waste is crossed grind mixing after, according to kitchen garbage and water 2:1 (mass ratio) mixes
(first is water, and the poor liquid with fermentation ends is needed to replace water circulate operation after distillation later), adds carbohydrase and makes every gram
Zymotic fluid is 50 enzyme activity, i.e. 50U/g is stirred evenly, and is placed in 60 DEG C of water-baths the 6h that is saccharified, and takes supernatant for sending out after centrifugation
Ferment.
(2) kitchen garbage supernatant (i.e. saccharified liquid) is put into fermentation tank, adjusts pH to 5, then (i.e. by the bacterial strain after activation
Yeast) (according to inoculum concentration 2%) addition fermentation tank, it controls temperature and ferments at 34 DEG C, fermentation time was controlled at 60 hours, was obtained
The yield for obtaining ethyl alcohol is 44g/L, and ferments and completed in left and right for 24 hours.
After fermentation, 80 DEG C of distillations processing is carried out to zymotic fluid, recycles ethyl alcohol, when in zymotic fluid concentration of alcohol down to not
Stop heating, storage tank liquid when can measure.
(3) distilled water is replaced to repeat step (1) and step (2) the poor liquid of gained in (2)；
(4) above-mentioned process is repeated five times, then by the poor liquid of circulating and recovering 5 times through 0.2 μm of inorganic ceramic membrane filtration
(can remove the impurity such as thalline and partially protein, pigment), obtained permeate enter three Room bipolar membrane electrodialysis reactors into
Row cycle, constant pressure (23.0V) operation, keeps temperature constant (30 DEG C) in the process, and each room flow rate of liquid 10L/h is surveyed every 20min
The concentration of each room pH, lactic acid and amino acid is measured by sampling per 40min for fixed each room pH value, wait in zymotic fluid lactic acid concn down to
Stop electrodialysis when 30g/L.
(5) repeat the above steps (2), i.e., fermentation tank is added in the yeast after activation, opens stirring, is saccharified and sends out into line asynchronous
Ferment producing and ethanol 36h, the yield for obtaining ethyl alcohol are 43.5g/L.
By comparison it can be found that after five cycles, fermentation time extended to 96 hours from 24 hours, concentration of alcohol
38g/L is reduced to by 44g/L, after electrodialysis, fermentation time foreshortened to 36 hours from 96 hours, and concentration of alcohol is by 38g/L
43.5g/L is risen to, ethanol production improves 15%.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, without departing from the principles of the present invention, several improvements and modifications can also be made, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (3)
1. a kind of alleviating method of the poor liquid reflux to the negative effect of kitchen waste ethanol fermentation, it is characterised in that：This method packet
Include following steps：
(1) bacterial strain activates：Angel dry ferment is put into the conical flask equipped with glucose solution, the Portugal that glucose solution is 2%
Grape sugar is added in deionized water and is made, and the adding proportion of Angel dry ferment is according to 20 times of extension rate, i.e. 1 gram of Angel dry ferment
It is put into 20 grams of glucose solutions, is placed in 35 DEG C of shaking table and cultivates 2-3h；
Kitchen garbage pre-processes：Kitchen waste is crossed grind mixing after, kitchen garbage is mixed with distilled water, adds carbohydrase,
It stirs evenly, is placed in 60 DEG C of water-baths the 6h that is saccharified, take supernatant for fermenting after centrifugation；
(2) supernatant in step (1) is put into fermentation tank, adjusts pH to 5-6, fermentation tank, control is added in the bacterial strain after activation
Temperature processed is fermented at 30-37 DEG C, and fermentation time was controlled at 36-96 hours, after fermentation, 80 DEG C of steamings is carried out to zymotic fluid
Evaporate processing, recycle ethyl alcohol, when in zymotic fluid concentration of alcohol down to stopping heating, storage tank liquid when can not measure；
(3) the poor liquid of gained in step (2) is substituted distilled water, repeats step for kitchen garbage preprocessing process in step (1)
(1) and step (2)；
(4) after poor liquid circulating and recovering 4-6 times, ethanol production had dropped 15-20% than original using distilled water, at this point, to most
The poor liquid of primary gained carries out electrodialysis process afterwards, and electrodialysis concrete operations are：Poor liquid obtains after 0.2 μm of inorganic ceramic membrane filtration
To permeate, permeate is recycled into three Room bipolar membrane electrodialysis devices, and electrodialytic process constant voltage operation is kept in the process
Temperature is constant, and each room flow rate of liquid of device is 10L/h, and each room pH value is measured every 20min, and each room is measured by sampling every 40min
The concentration of pH, lactic acid and amino acid stop electrodialysis, after obtaining electrodialysis when lactic acid concn in permeate is down to 20-40g/L
Poor liquid, poor liquid replaces distilled water to be used for step (1) again after electrodialysis；
The volume mass ratio of inoculation amount is 2%~4% in the step (2)；
In the step (4), when electrodialysis, constant pressure 23.0V, temperature is constant at 30 DEG C.
The method of the negative effect to kitchen waste ethanol fermentation 2. the poor liquid of a kind of alleviation according to claim 1 flows back,
It is characterized in that：In the step (1), when kitchen garbage pre-processes, kitchen garbage is with distilled water according to 2:1~1:1 quality
Than mixing, in continuous operation, poor liquid replaces distilled water, and mixing quality is than constant.
The method of the negative effect to kitchen waste ethanol fermentation 3. the poor liquid of a kind of alleviation according to claim 1 flows back,
It is characterized in that：In the step (1), when kitchen garbage pre-processes, the ratio that adds of carbohydrase be every gram of zymotic fluid is 50-
100 enzyme activity, i.e. 50-100U/g.
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| CN111118069A (en) * | 2019-12-18 | 2020-05-08 | 长沙凯天工研院环保服务有限公司 | Method for producing bioethanol by anaerobic fermentation of kitchen waste |
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| CN1948498A (en) * | 2006-10-24 | 2007-04-18 | 北京科技大学 | Method of producing fuel alcohol by kitchen garbage fermentation |
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| CN1948498A (en) * | 2006-10-24 | 2007-04-18 | 北京科技大学 | Method of producing fuel alcohol by kitchen garbage fermentation |
Non-Patent Citations (5)
| Title |
|---|
| A novel full recycling process through two-stage anaerobic treatment of distillery wastewater for bioethanol production from cassava.;Qing-Hua Zhang et al.;《Journal of Hazardous Materials》;20101231;第179卷;635-641 * |
| Biological flocculation treatment on distillery wastewater and recirculation of wastewater.;Wen Zhang et al.;《Journal of Hazardous Materials》;20091231;第172卷;1252-1257 * |
| Distillery waste recycle through membrane filtration in batch alcohol fermentation.;Jae-Sok Kim et al.;《Biotechnology Letters》;19991231;第21卷;401-405 * |
| Wojciech Białas et al..Fuel ethanol production from granular corn starch using Saccharomyces cerevisiae in a long term repeated SSF process with full stillage recycling..《Bioresource Technology》.2010,第101卷3126-3131. * |
| 餐厨垃圾乙醇发酵副产物资源化利用及其机理研究;苏伟;《北京科技大学博士学位论文》;20111231;摘要 * |
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