CN105110911A - Preparation method for coprinus comatus culture medium - Google Patents
Preparation method for coprinus comatus culture medium Download PDFInfo
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- CN105110911A CN105110911A CN201510491422.8A CN201510491422A CN105110911A CN 105110911 A CN105110911 A CN 105110911A CN 201510491422 A CN201510491422 A CN 201510491422A CN 105110911 A CN105110911 A CN 105110911A
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- fermentation
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Abstract
The invention discloses a preparation method for a coprinus comatus culture medium. The preparation method comprises: firstly fermenting earthworms to prepare an earthworm fertilizer; preparing a culture medium to provide abundant trace elements and amino acids to coprinus comatus; as the fermenting temperature of other raw materials is overhigh, in order to prevent nutritional elements in an earthworm liquid from being damaged, firstly fermenting the earthworm liquid; and then fermenting the earthworm liquid with other raw materials together, wherein the nutritional elements of the earthworm liquid are absorbed in a biologically absorbable form to provide nutrition to coprinus comatus, so that micro elements are enriched.
Description
Technical field
The invention belongs to planting edible mushroom field, be specifically related to a kind of preparation method of Coprinus comatus substratum.
Background technology
Coprinus comatus has another name called Shaggy Mane, is being commonly called as of coprinus comatus, and because its shape is as chicken leg, meat meat flavour is gained the name like shredded chicken, is the rare bacterium product with business potential of artificial exploitation in recent years, is described as " in bacterium rising star ".
Coprinus comatus is nutritious, delicious flavour, and mouthfeel is fabulous, often ediblely contributes to improving a poor appetite, digests, strengthens body immunity, have very high nutritive value.
Containing crude protein 25.4g, fatty 3.3g, total reducing sugar 58.8g in the dry mushroom of every 100g, also containing multiple amino acids in Coprinus comatus, comprise the amino acid of 8 kinds of needed by human.Coprinus comatus is again a kind of medicinal fungi, has aid digestion, strengthening the spleen and stomach, improves the functions such as body immunity, the dark favor by domestic and international human consumer.
Mushroom body is pure white, attractive in appearance, and meat is fine and smooth.Stir-fry and eat, boiled dinner, Baoshang boils not rotten all for a long time, and mouthfeel is sliding tender, and delicate fragrance is delicious, thus extremely consumers.The sweet property of Coprinus comatus taste is put down, useful taste, clear away heart-fire allay excitement, aid digestion, increase appetite, the effect such as hypotensive, antitumor.Often eat and can improve immune function of human body, strengthen resistance against diseases.Containing multiple bioactive enzyme in fresh mushroom, have multiple physiological action, wherein trypsinase, maltin etc. can help digest, and tyrosine oxidase has the effect reduced blood pressure, and compound of polysaccharide then has certain anti-cancer and kill cancer action.Fat contained in mushroom body mostly is unsaturated fatty acids, can not increase the cholesterol level in blood after edible, can prevention of arterial sclerosis, heart trouble and obesity etc.Coprinus comatus also has certain restraining effect to intestinal bacteria, streptococcus aureus, Bacillus subtilus.In addition, the effective constituent also containing treatment diabetes in Coprinus comatus, can reduce blood sugar concentration after edible, have obvious auxiliary curative effect to diabetes.
Due to the nutrition of Coprinus comatus collection, health care, dietotherapy, has high protein, low-fat good characteristic.And look, fragrant, taste shape is all good, thus extremely consumers.And Coprinus comatus bag growth cycle is short, biological transformation ratio is higher, is easy to cultivation, is particularly suitable for Chinese countryside plantation.Planting scale rapidly expands in recent years, has become one of edible mushrooms of the large cultivation of Agaricales China.
But the Coprinus comatus micronutrient levels of tradition plantation is few, can not meet the health care demand of people.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of preparation method of Coprinus comatus substratum, preparing Rich in Trace Element, comprehensive, balanced nutrition can not only be provided to promote that it grows to Coprinus comatus, and, make Coprinus comatus rich trace elements, have higher edible and health value.
Described Coprinus comatus substratum, comprises the raw material of following weight part:
Described earthworm liquid is evenly pulled an oar in electric mixer by live body earthworm, obtains earthworm slurries;
Described fermentation base is EM bacterium;
Described silkworm excrement refers to: the solarization of fresh fifth instar larva sand obtained for 2-3 days, with drying sterilizing;
A preparation method for Coprinus comatus substratum, comprises the following steps:
(1), by the earthworm liquid of 3-4 weight part mix with the bacterium liquid of 0.03-0.06 weight part, at 40 DEG C of fermentation 35-40 hour, obtain fermentation earthworm liquid;
(2), by fermentation earthworm liquid and 20-30 weight part cotton seed hulls, 0.5-0.8 weight part amino acid, 0.8-1.3 weight part sugar slag put into fermentation cauldron, after fermentation, obtain earthworm fertilizer;
(3), the lime of the Coprinus comatus Waste compost of the dregs of beans of the rice bran of the silkworm excrement of 6-9 weight part, 20-30 weight part, 10-15 weight part, 10-15 weight part, 2-3 weight part, the calcium superphosphate of 0.5-1 weight part are added 40-60 weight parts water and stirs, mixes, control water content at 45-50%, fermentation 6-7 days;
(4), add earthworm fertilizer prepared by step (2), stirring and evenly mixing, fermentation 6-8d, obtains Coprinus comatus substratum.
Ferment described in step (3) and be specially: first at 40-50 DEG C of condition bottom fermentation 1-2 days, turning; Improve temperature to 60 DEG C again, ferment 2 days, turning; Reduce temperature to 55 DEG C again, ferment 3 days.
Fermentation is divided into three sections to carry out, and first at 40-50 DEG C of bottom fermentation 1-2 days, ferments at a lower temperature, prevents temperature in fermenting process too high, the enzyme meeting inactivation in microbe, follow-up the carrying out of impact fermentation; Temperature is too low, and the enzymatic activity in microbe is inadequate again, is unfavorable for the carrying out of fermenting; Select 40-50 DEG C of fermentation, play the maximum catalytic activity of the enzyme in microbe, be beneficial to the carrying out of subsequent fermentation; Due to the carrying out of fermentation, substratum internal temperature improves, and for ensureing that fermentation evenly, improves temperature to 60 DEG C, promotes that fermentation is carried out; After turning, be finally cooled to 55 DEG C, prevent the nutritive ingredient of the too high destruction substratum of temperature.
In step (4), fermentation condition is: 50-60 DEG C of fermentation 6-8d, in order to ensure that earthworm fertilizer Middle nutrition element is not destroyed, leavening temperature is unsuitable too high.
The protein content of earthworm accounts for 53.5% ~ 65.1% of dry weight, and lipid content is about 4.4% ~ 17.38%, and carbohydrate is about 11% ~ 17.4%, ash content 7.8% ~ 23%.Also containing abundant vitamins D (accounting for 0.04% ~ 0.073% of fresh body weight) in earthworm body, and the mineral element such as calcium and phosphorus (accounting for 0.124% ~ 0.188% of fresh body weight).Containing the multiple medicinal ingredients such as earthworm element, earthworm toxolysin, xanthine, antihistaminic and vitamins B in earthworm body.Meanwhile, earthworm is also some parasite as the intermediate host of nematode and tapeworm, as strongylid after ring fine rule worm and lunge etc.
In the present invention, first fermented by earthworm, obtained earthworm fertilizer, makes substratum, to be supplied to the abundant trace element of Coprinus comatus and amino acid.The leavening temperature of other raw materials is too high, for preventing earthworm liquid nutritive element to be destroyed, is first fermented by earthworm liquid, ferment together with other raw materials after fermentation again, its nutritive element is absorbed with the absorbable form of biology, for Coprinus comatus provides nutrition, makes its Rich in Trace Element.
Silkworm excrement comprehensive nutrition, dry silkworm excrement contains crude protein 13.4%-14.5%, crude fat 2.2%-2.3%, robust fibre 15.8%-16.2%, nitrogen-free extract 56.9%-57.4%, the also trace element such as cupric, iron and zinc, and containing plant growth hormoness such as a small amount of alkaloid, class adrenocortical hormone, Va, Vb, Vc, Vd, Ve, nicotinic acid, indolylacetic acid and triacontanol price quotes.The present invention utilizes silkworm excrement, acts synergistically with earthworm liquid, jointly for Coprinus comatus provides abundant trace element, improves the content of its trace element.
Compared with prior art, substratum comprehensive nutrition provided by the invention, rich trace elements, and also biological transformation ratio is high; According to the characteristic of Coprinus comatus, design fermentation condition, makes substratum balanced in nutrition, promotes Coprinus comatus growth.Grow that the Coprinus comatus that obtains is balanced in nutrition, rich trace elements and amino acid, have higher practical value and health value.
Embodiment
Embodiment 1
A preparation method for Coprinus comatus substratum, comprises the following steps:
(1), by the earthworm liquid of 3 weight parts mix with the bacterium liquid of 0.03 weight part, at 40 DEG C of fermentation 35-40 hour, obtain fermentation earthworm liquid;
(2), by fermentation earthworm liquid and 20 weight part cotton seed hullss, 0.5 weight part amino acid, 0.8 weight part sugar slag put into fermentation cauldron, after fermentation, obtain earthworm fertilizer;
(3), the lime of the Coprinus comatus Waste compost of the dregs of beans of the rice bran of the silkworm excrement of 6 weight parts, 21 weight parts, 10 weight parts, 12 weight parts, 2 weight parts, the calcium superphosphate of 0.5 weight part are added 40 weight parts waters and stirs, mixes, control water content at 45-50%, first at 40-50 DEG C of condition bottom fermentation 1-2 days, turning; Improve temperature to 60 DEG C again, ferment 2 days, turning; Reduce temperature to 55 DEG C again, ferment 3 days.
(4), add earthworm fertilizer prepared by step (2), stirring and evenly mixing, 50-60 DEG C of fermentation 6 days, obtains Coprinus comatus substratum.
Embodiment 2
A preparation method for Coprinus comatus substratum, comprises the following steps:
(1), by the earthworm liquid of 4 weight parts mix with the bacterium liquid of 0.046 weight part, at 40 DEG C of fermentation 35-40 hour, obtain fermentation earthworm liquid;
(2), by fermentation earthworm liquid and 25 weight part cotton seed hullss, 0.7 weight part amino acid, 0.9 weight part sugar slag put into fermentation cauldron, after fermentation, obtain earthworm fertilizer;
(3), the lime of the Coprinus comatus Waste compost of the dregs of beans of the rice bran of the silkworm excrement of 8 weight parts, 25 weight parts, 11 weight parts, 13 weight parts, 2 weight parts, the calcium superphosphate of 0.6 weight part are added 40 weight parts waters and stirs, mixes, control water content at 45-50%, first at 40-50 DEG C of condition bottom fermentation 1-2 days, turning; Improve temperature to 60 DEG C again, ferment 2 days, turning; Reduce temperature to 55 DEG C again, ferment 3 days.
(4), add earthworm fertilizer prepared by step (2), stirring and evenly mixing, 50-60 DEG C of fermentation 8 days, obtains Coprinus comatus substratum.
Embodiment 3
A preparation method for Coprinus comatus substratum, comprises the following steps:
(1), by the earthworm liquid of 4 weight parts mix with the bacterium liquid of 0.06 weight part, 40 DEG C of fermentations 40 hours, obtain fermentation earthworm liquid;
(2), by fermentation earthworm liquid and 30 weight part cotton seed hullss, 0.8 weight part amino acid, 1.3 weight part sugar slags put into fermentation cauldron, after fermentation, obtain earthworm fertilizer;
(3), the lime of the Coprinus comatus Waste compost of the dregs of beans of the rice bran of the silkworm excrement of 9 weight parts, 290 weight parts, 14 weight parts, 14 weight parts, 3 weight parts, the calcium superphosphate of 0.9 weight part are added 56 weight parts waters and stirs, mixes, control water content at 45-50%, first 40-50 DEG C of condition bottom fermentation 2 days, turning; Improve temperature to 60 DEG C again, ferment 2 days, turning; Reduce temperature to 55 DEG C again, ferment 3 days.
(4), add earthworm fertilizer prepared by step (2), stirring and evenly mixing, 50-60 DEG C of fermentation 8 days, obtains Coprinus comatus substratum.
Compared with the Coprinus comatus of the substratum plantation utilizing embodiment 1-3 to prepare and the tradition Coprinus comatus nutritive element content of planting, (every 100 Coprinus comatus dry products) result is as following table 1:
Table 1
| Constituent content | Embodiment 1 | Embodiment 2 | Embodiment 3 | Tradition |
| Protein g | 26.8 | 27.1 | 28.1 | 24.5 |
| Fiber g | 8 | 9.5 | 8.2 | 7.1 |
| Potassium mg | 1789.2 | 1836.5 | 1899.2 | 1661.9 |
| Sodium mg | 51.3 | 56.2 | 48.9 | 34.01 |
| Calcium mg | 112.6 | 131.1 | 123.5 | 106.7 |
| Magnesium mg | 278.3 | 267.1 | 250.2 | 191.47 |
| Phosphorus mg | 780.3 | 801.5 | 791.3 | 634.1 |
| Iron mg | 1.723 | 1.636 | 1.683 | 1.376 |
| Zinc mg | 0.14 | 0.13 | 0.12 | 0.09 |
Claims (6)
1. a preparation method for Coprinus comatus substratum, is characterized in that, described preparation method comprises the following steps:
(1), by the earthworm liquid of 3-4 weight part mix with the bacterium liquid of 0.03-0.06 weight part, at 40 DEG C of fermentation 35-40 hour, obtain fermentation earthworm liquid;
(2), by fermentation earthworm liquid and 20-30 weight part cotton seed hulls, 0.5-0.8 weight part amino acid, 0.8-1.3 weight part sugar slag put into fermentation cauldron, after fermentation, obtain earthworm fertilizer;
(3), the lime of the Coprinus comatus Waste compost of the dregs of beans of the rice bran of the silkworm excrement of 6-9 weight part, 20-30 weight part, 10-15 weight part, 10-15 weight part, 2-3 weight part, the calcium superphosphate of 0.5-1 weight part are added 40-60 weight parts water and stirs, mixes, control water content at 45-50%, fermentation 6-7 days;
(4), add earthworm fertilizer prepared by step (2), stirring and evenly mixing, fermentation 6-8d, obtains Coprinus comatus substratum.
2. preparation method according to claim 1, is characterized in that, fermenting described in step (3) is specially: first at 40-50 DEG C of condition bottom fermentation 1-2 days, turning; Improve temperature to 60 DEG C again, ferment 2 days, turning; Reduce temperature to 55 DEG C again, ferment 3 days.
3. preparation method according to claim 1 and 2, is characterized in that, in step (4), fermentation condition is: 50-60 DEG C of fermentation 6-8d.
4. preparation method according to claim 1 and 2, is characterized in that, described earthworm liquid is evenly pulled an oar in electric mixer by live body earthworm, obtains earthworm slurries.
5. preparation method according to claim 1 and 2, is characterized in that, fermentation base is EM bacterium.
6. preparation method according to claim 1 and 2, is characterized in that, described silkworm excrement refers to: the solarization of fresh fifth instar larva sand obtained for 2-3 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113273433A (en) * | 2021-06-04 | 2021-08-20 | 湖南省林业科学院 | Coprinus comatus culture medium taking oil-tea camellia cake as raw material and preparation method thereof |
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| CN101897273A (en) * | 2010-07-19 | 2010-12-01 | 青岛农业大学 | Coprinus comatus cultivating method and cultivating medium |
| CN103848654A (en) * | 2014-03-26 | 2014-06-11 | 安徽利海新城镇开发有限公司 | Preparation method of earthworm liquid organic fertilizer |
| CN104544395A (en) * | 2014-12-12 | 2015-04-29 | 德清奥元生物科技有限公司 | Plant and fruit fermentation stock solution |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897273A (en) * | 2010-07-19 | 2010-12-01 | 青岛农业大学 | Coprinus comatus cultivating method and cultivating medium |
| CN103848654A (en) * | 2014-03-26 | 2014-06-11 | 安徽利海新城镇开发有限公司 | Preparation method of earthworm liquid organic fertilizer |
| CN104544395A (en) * | 2014-12-12 | 2015-04-29 | 德清奥元生物科技有限公司 | Plant and fruit fermentation stock solution |
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| 周元军编著: "《图说蝎子养殖技术 第2版》", 31 March 2014 * |
| 方海兰等编著: "《城市土壤生态功能与有机废弃物循环利用》", 31 January 2014 * |
| 湖南农学院编: "《土壤肥料基础知识（初级本）》", 30 September 1984 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113273433A (en) * | 2021-06-04 | 2021-08-20 | 湖南省林业科学院 | Coprinus comatus culture medium taking oil-tea camellia cake as raw material and preparation method thereof |
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Application publication date: 20151202 |