CN105104767A - Leech envelope mould infection prevention and control feed and preparation method thereof - Google Patents

Abstract

The present invention discloses a leech envelope mould infection prevention and control feed, which is prepared from the following raw materials by weight: 30-40 parts of biscuit residue, 10-13 parts of soybean hull, 10-12 parts of tea egg yolk, 30-40 parts of chicken bone soup, 40-50 parts of porcine plasma, 5-8 parts of cheese, 40-50 parts of amaranthus hypochondriacus, 30-40 parts of tenebrio molitor, 5-8 parts of plasma protein powder, 10-15 parts of sodium bicarbonate water, a proper amount of nystatin, a proper amount of edible oil, a proper amount of compound cellulase, a proper amount of yeast, a proper amount of Bacillus natto, a proper amount of sodium hydroxide, a proper amount of chitosan, and a proper amount of water. According to the present invention, the tenebrio molitor and the amaranthus hypochondriacus are subjected to extraction, centrifugation fermentation and other processes to prepare the protein peptide easily digested and absorbed by leeches, the good physiological function is provided, the feed has characteristics of fragrant taste and easy storage, the mould infection rate of the fed leeches is substantially reduced, and the fed leeches have characteristics of good growth, long and full body, and positive eating.

Description

A kind of control leech mycotic infection coating feed and preparation method thereof

Technical field

The present invention relates to a kind of field of feed, particularly one control leech mycotic infection coating feed and preparation method thereof.

Background technology

China utilizes leech nearly one thousand years more Zao than west, and leech begins to remember that history tree is all on the books in " legendary god of farming's book on Chinese herbal medicine " as Chinese medicine.In national academic conference promoting blood circulation and removing blood stasis in 1986, leech is confirmed as one of 35 kinds of Chinese medicines promoting blood circulation and removing blood stasis.Englishman Mike watt finds and the hirudin extracted the 1950's, and a few microgram just can make blood solidify, and it can reduce blood viscosity, alleviation arterial wall spasm, hemangiectasis, stimulates circulation and absorb exudate.Hirudin also can be used for treating miocardial infarction, headstroke, thrombus class angiosis and vascular hypertension.Surgery removes hemostasis after being used for reconstructive operation, is the jinx of the thrombotic diseases such as cardiovascular disease.Leech vitality is extremely strong, substantially disease-free, but strict to water quality requirement.Water body requires that water source is avoided containing strong iron and sulphur matter, can survive in the waters of pH value 6.4-9 without industry and domestic pollution, but can not live in the acid waters that pH value is extremely low.The leech annual 3-6 month breeds, and lays eggs cocoon 2-3, young leech 13-35 bar in each cocoon, most 20 at every turn.

Yellow meal worm is a kind of important storage pest be distributed in all over the world, and be commonly called as bread worm, indoor feeding is extremely easy.Being of high nutritive value of yellow meal worm, have the health-care effects such as antifatigue, anti-ageing, anti-sudden change, adjusting blood lipid and immunity moderation function, wherein to reach 56.58%, 18 seed amino acids complete for crude protein content, its essential amino acids content accounts for 39.14% of total amino acid content, is a kind of protein resource of high-quality.Grain Production of Amaranthus is annual grain, the feed dual-purpose crop of Amaranthaceae Amaranthus.Protein content in Grain Production of Amaranthus amaranth seed and quality are all higher than general cereal, and amino acid proportion balance.Protein is extracted from yellow meal worm and Grain Production of Amaranthus, then for the coating of feed after fermentation.

Summary of the invention

The present invention compensate for the deficiencies in the prior art, provides a kind of control leech mycotic infection coating feed and preparation method thereof.

Technical scheme of the present invention is as follows:

Leech feed of the present invention is made up of the raw material of following weight portion: biscuit slag 30-40, soybean skin 10-13, tealeaves yolk 10-12, chicken bone soup 30-40, Swine plasma 40-50, cheese 5-8, Grain Production of Amaranthus 40-50, yellow meal worm 30-40, SDPP 5-8, sodium bicarbonate water 10-15, nystatin, edible oil, complex cellulase, saccharomycete, Bacillus natto, NaOH, shitosan, water are appropriate;

The concrete steps of the preparation of described leech feed are as follows:

(1) Grain Production of Amaranthus is ground into Powdered, adds the water yield of 20 times, with NaOH adjust pH to 9, under 30 DEG C of conditions, vibration insulation 1h, takes out and leaves standstill 1-2 minute, centrifugation 10 minutes, speed per hour is 1500 revs/min, is separated to obtain Grain Production of Amaranthus protein liquid and Grain Production of Amaranthus sediment;

(2) make powder by after yellow meal worm microwave drying, put into the sodium hydroxide solution of 1.5mol/L, 70 DEG C of Water Unders bathe reaction 6 hours, and centrifugation 15 minutes, obtains yellow mealworm protein liquid and sediment;

(3) the Grain Production of Amaranthus protein liquid of step 1, the yellow mealworm protein liquid of step 2, SDPP, Bacillus natto are mixed, regulate PH to be 7.2,38 DEG C of condition bottom fermentations 32 hours, obtain protein peptides liquid;

(4) soybean skin being added suitable quantity of water and complex cellulase, is 40 DEG C in temperature, and ultrasonic frequency is carry out enzyme process ultrasonic assistant pretreatment 1 hour, separating filtrate and filter residue under 40KH condition;

(5) by biscuit slag, tealeaves yolk, chicken bone soup mixing infusion 10-15 minute, mix with the Grain Production of Amaranthus sediment of step 1, the yellow meal worm sediment of step 2, the filter residue of step 4, saccharomycete again, sealing, normal temperature bottom fermentation 20-24 hour, then nystatin is added suitable quantity of water stir, then microwave treatment 4-5 minute after mixing with the filter residue of step 4, mix with above-mentioned fermentation materials again, dry, granulation, obtains hybrid particles;

(6) Swine plasma, cheese are mixed, heating is stirred to cheese and melts completely, then mixes with the filtrate of the protein peptides liquid of step 3, step 4, edible oil and other residual components, is more evenly sprayed on the hybrid particles of step 5, dries.

Beneficial effect of the present invention:

In the present invention, SDPP not only can provide a large amount of nutritional needs, the smell of blood of feed can also be increased, take food in a large number when luring leech to eat food, adopt the multiple enzyme system such as Bacillus natto extracellular proteinase and cellulase to be hydrolyzed the protein liquid extracted from yellow meal worm and Grain Production of Amaranthus and be prepared into low molecular protein peptide, low molecular protein peptide has promotion lipid-metabolism, reduces all more important physiological functions such as cholesterol and blood pressure, anti-oxidant, antifatigue, develop immunitypty, promotion wound healing.Again by Grain Production of Amaranthus, yellow meal worm sediment and other composition mixing after fermentation process, not only improve the nutritive value of feed, all right hyperchromic flavouring, improves the appetite of leech.Soybean skin is formed water-soluble dietary fiber after compound cellulose ferment treatment, makes the beneficial mattress activate in enteron aisle, promote beneficial bacterium amount reproduction, there is stickiness, can nystatin be adsorbed, make its slow releasing efficacy of a drug, improve the mouthfeel that soybean skin is coarse simultaneously.

In feed yellow meal worm and Grain Production of Amaranthus by extracting, the technique such as centrifugal fermentation prepares more easily by the protein peptides that leech digests and assimilates, and has better physiological function, feed taste is fragrant, storage easily.The leech mycotic infection rate of raising out reduces greatly, leech well-grown, and the oval profit of body, feed are actively.

Specific embodiments

Below in conjunction with following detailed description of the invention, the present invention is described in further detail:

The raw material taking following weight portion (kg) is made: biscuit slag 35, soybean skin 12, tealeaves yolk 12, chicken bone soup 35, Swine plasma 45, cheese 7, Grain Production of Amaranthus 47, yellow meal worm 30, SDPP 7, sodium bicarbonate water 10-15, nystatin, edible oil, complex cellulase, saccharomycete, Bacillus natto, NaOH, shitosan, water are appropriate;

The concrete steps of the preparation method of leech feed are as follows:

(1) Grain Production of Amaranthus is ground into Powdered, adds the water yield of 20 times, with NaOH adjust pH to 9, under 30 DEG C of conditions, vibration insulation 1h, takes out and leaves standstill 2 minutes, centrifugation 10 minutes, speed per hour is 1500 revs/min, is separated to obtain Grain Production of Amaranthus protein liquid and Grain Production of Amaranthus sediment;

(2) make powder by after yellow meal worm microwave drying, put into the sodium hydroxide solution of 1.5mol/L, 70 DEG C of Water Unders bathe reaction 6 hours, and centrifugation 15 minutes, obtains yellow mealworm protein liquid and sediment;

(3) the Grain Production of Amaranthus protein liquid of step 1, the yellow mealworm protein liquid of step 2, SDPP, Bacillus natto are mixed, regulate PH to be 7.2,38 DEG C of condition bottom fermentations 32 hours, obtain protein peptides liquid;

(4) soybean skin being added suitable quantity of water and complex cellulase, is 40 DEG C in temperature, and ultrasonic frequency is carry out enzyme process ultrasonic assistant pretreatment 1 hour, separating filtrate and filter residue under 40KH condition;

(5) biscuit slag, tealeaves yolk, chicken bone soup are mixed infusion 12 minutes, mix with the Grain Production of Amaranthus sediment of step 1, the yellow meal worm sediment of step 2, saccharomycete again, sealing, normal temperature bottom fermentation 22 hours, then nystatin is added suitable quantity of water stir, then microwave treatment 5 minutes after mixing with the filter residue of step 4, mix with above-mentioned fermentation materials again, dry, granulation, obtains hybrid particles;

(6) Swine plasma, cheese are mixed, heating is stirred to cheese and melts completely, then mixes with the filtrate of the protein peptides liquid of above-mentioned steps 3, step 4, edible oil and other residual components, is more evenly sprayed on the hybrid particles of step 5, dry.

In order to further illustrate using value of the present invention, implement people and leech is equally divided into experimental group and control group two groups according to age in days, body weight, principle that health status is identical by 200 leech, experimental group feeds leech feed of the present invention, control group feeds traditional leech feed, and experimental period is 3 months, and result of the test is that the feed of experimental group leech is active, survival rate improves 12.3%, efficiency of feed utilization improves 15.6%, and daily gain improves 7.8%, and mycotic infection rate reduces by 7.6%.

Claims (2)

1. a control leech mycotic infection coating feed, it is characterized in that, be made up of the raw material of following weight portion: biscuit slag 30-40, soybean skin 10-13, tealeaves yolk 10-12, chicken bone soup 30-40, Swine plasma 40-50, cheese 5-8, Grain Production of Amaranthus 40-50, yellow meal worm 30-40, SDPP 5-8, sodium bicarbonate water 10-15, nystatin, edible oil, complex cellulase, saccharomycete, Bacillus natto, NaOH, shitosan, water are appropriate.

2. prevent and treat leech mycotic infection coating feed according to claim 1, it is characterized in that, the concrete steps of preparation method are as follows:

(1) Grain Production of Amaranthus is ground into Powdered, adds the water yield of 20 times, with NaOH adjust pH to 9, under 30 DEG C of conditions, vibration insulation 1h, takes out and leaves standstill 1-2 minute, centrifugation 10 minutes, speed per hour is 1500 revs/min, is separated to obtain Grain Production of Amaranthus protein liquid and Grain Production of Amaranthus sediment;

(2) make powder by after yellow meal worm microwave drying, put into the sodium hydroxide solution of 1.5mol/L, 70 DEG C of Water Unders bathe reaction 6 hours, and centrifugation 15 minutes, obtains yellow mealworm protein liquid and sediment;

(3) the Grain Production of Amaranthus protein liquid of step 1, the yellow mealworm protein liquid of step 2, SDPP, Bacillus natto are mixed, regulate PH to be 7.2,38 DEG C of condition bottom fermentations 32 hours, obtain protein peptides liquid;

(4) soybean skin being added suitable quantity of water and complex cellulase, is 40 DEG C in temperature, and ultrasonic frequency is carry out enzyme process ultrasonic assistant pretreatment 1 hour, separating filtrate and filter residue under 40KH condition;

(5) by biscuit slag, tealeaves yolk, chicken bone soup mixing infusion 10-15 minute, mix with the Grain Production of Amaranthus sediment of step 1, the yellow meal worm sediment of step 2, the filter residue of step 4, saccharomycete again, sealing, normal temperature bottom fermentation 20-24 hour, then nystatin is added suitable quantity of water stir, then microwave treatment 4-5 minute after mixing with the filter residue of step 4, mix with above-mentioned fermentation materials again, dry, granulation, obtains hybrid particles;

(6) Swine plasma, cheese are mixed, heating is stirred to cheese and melts completely, then mixes with the filtrate of the protein peptides liquid of step 3, step 4, edible oil and other residual components, is more evenly sprayed on the hybrid particles of step 5, dries.