CN105092545A - UV-fluorescent label detection method for environment sanitation and uses - Google Patents
UV-fluorescent label detection method for environment sanitation and uses Download PDFInfo
- Publication number
- CN105092545A CN105092545A CN201410202946.6A CN201410202946A CN105092545A CN 105092545 A CN105092545 A CN 105092545A CN 201410202946 A CN201410202946 A CN 201410202946A CN 105092545 A CN105092545 A CN 105092545A
- Authority
- CN
- China
- Prior art keywords
- environment
- fluorescent reagent
- fluorescent
- detection method
- cleaning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a UV-fluorescent label detection method for environment sanitation and uses. The method comprises the following steps: S1, labeling the surface of an article in an environment with a fluorescent reagent before cleaning the environment; and S2, detecting by UV if a fluorescent reagent label is removed after the cleaning. If the fluorescent reagent label is removed, the cleaning work is qualified; if the fluorescent reagent label is not removed or partly removed, the cleaning work is not qualified. The fluorescent reagent is composed of the following ingredients by weight: 1% of ethylene glycol phenyl ether, 10-20% of N-(2-ethoxyl)-N-(2-ethylhexyl)B-alanine mono sodium salt, 1-5% of isopropanol, 1-5% of C9-11 alkanol polyether-3, 1-5% of PEG-2 cocomethyl ammonium chloride, 1-5% ethoxylated isodecanol, 4,4-di(2-sulfonylstyrene)biphenyl disodium salt, and 2-5% of nanometer silver, balance being water. The UV-fluorescent label detection method can be used for quickly detecting medical environment, lab environment and hotel environment; the detection cost is low, and the detection result can be fed back to intervene the cleaning.
Description
Technical field
The present invention relates to a kind of environmental health ultraviolet fluorescent mark detection method and application.
Background technology
Be well known that, comprise for sanitary evaluation method at present: observation method of naked eye, Microbiological Lab's cultivation, ATP bioluminescence method.All there is drawback in above-mentioned each evaluation method:
1, observation method of naked eye
Its drawback one is, is mainly to belong to subjective judgement;
Its drawback two is, is macroscopicly limited in scope, and in such as environment, bacterium directly with the naked eye to observe.
2, Microbiological Lab's cultivation
Its drawback one is, microorganism incubation time is long, at least needs 24 hours or more;
Its drawback two is, is not suitable for large-scale evaluation and utilization, if the scope that environmental health is evaluated is comparatively large, then the method cannot meet the requirement that the short time provides evaluation;
Its drawback three is, nutrient culture media costly, causes its operation strategies limited.
3, ATP bioluminescence method
Its drawback one is, because it is without the standard evaluated, causes standard to differ;
Its drawback two is, specificity and susceptibility poor;
Its drawback three is, swab price is higher.
Summary of the invention
Object of the present invention is exactly to solve the problem, a kind of environmental health ultraviolet fluorescent mark detection method and application are provided, the method can the sanitary cleaning effect of objective detection, and avoid the cross-infection that Reusability in testing process causes, fed back by testing result and intervene cleaning course, effective raising environmental health clean level, reduces microorganisms spreading probability.
To achieve these goals, the present invention adopts following technical scheme:
Environmental health ultraviolet fluorescent mark detection method, the method comprises the steps:
S1., before to clean environment, fluorescent reagent is marked on environmental objects surface;
S2., after having cleaned, UV detection fluorescent reagent is utilized to mark whether to be eliminated;
If fluorescent reagent mark is eliminated, then cleaning is qualified, judges that environmental health is qualified;
If fluorescent reagent mark is not eliminated or part is eliminated, then cleaning is defective, judges that environmental health is defective;
Wherein, described fluorescent reagent is made up of the component of following weight percents:
Described fluorescent reagent is made up of the component of following weight percents:
Described fluorescent reagent is made up of the component of following weight percents:
The application of environmental health ultraviolet fluorescent mark detection method, the application of this environmental health ultraviolet fluorescent mark detection method applies to medical environment detection, laboratory environment detects, hotel environment detects, wherein, detect object and comprise microorganism, organism, inorganics.
Fluorescent reagent as previously described, its in appearance visual inspection be colourless transparent liquid or gel, ultraviolet light 200-400nm irradiate excite under send visible ray.
The present invention has following beneficial effect:
(1) the method detects for medical environment, laboratory environment detects, hotel environment detect have fast, advantage easily.
(2) the method has objective examination criteria, if fluorescence labeling occurs, judges sanitary clean defective, otherwise then qualified.
(3) the method has clear and definite baseline criteria, prevents varying environment, different personnel produce objection for criterion of acceptability.
(4) reagent that the method is used in testing process has feature at a low price, is applicable to scale and uses.
(5) carrier (such as spongy top) of reagent place's carrying of the method can Reusability, without the problem of cross pollution.
By the detection for result, cleaning course can be intervened.
Embodiment
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.
For medical environment, sanitary inspection personnel are before staff is clean, fluorescent reagent utilized the first-class carrier of such as sponge and be marked on the surface at public ward bedside cupboard top, here, the place of fluorescent reagent mark is certain fixed position of the crucial high-frequency resistance body surface of regulation in advance, and the material of fluorescent reagent marking objects includes but not limited to wooden, metal, plastics, pottery, rubber.Due to fluorescent reagent be labeled after be sightless under naked eyes, environmental health cleanup crew does not know some crucial high-frequency resistance objects in environment and has been fluorescently labeled and marks, after environmental health cleanup crew has cleaned, sanitary inspection personnel mark whether thoroughly to be removed with UV detection fluorescent reagent, if mark is thoroughly removed, judge that cleaning is qualified, and judge that environmental health is qualified; Then be judged to be that cleaning is defective as thoroughly do not removed mark, corresponding environmental health is defective.
This fluorescent reagent can repeatedly mark at body surface on the first-class carrier of such as sponge, and does not occur cross-infection problem, and this and the Nano Silver used by fluorescent reagent are closely related.
It should be noted that each component of this fluorescent reagent includes but not limited to following disclosed percentage by weight type: the ethylene glycol phenyl ether of 1%; N-(2-hydroxyethyl)-N-(2-ethylhexyl) the B-alanine list sodium salt of 15%; The isopropyl alcohol of 2%; The C9-11 Pareth-3 of 4%; The PEG-2 cocoyl methyl ammonium chloride of 3%; The isomery ten alcohol polyoxyethylene ether of 3%; 4,4-two (2-sulfonated styrene) biphenyl disodium salts of 12%; The Nano Silver of 2%; Surplus is deionized water.
Other example is made to the component of fluorescent reagent and percentage by weight below:
The ethylene glycol phenyl ether of example 1:1%; N-(2-hydroxyethyl)-N-(2-ethylhexyl) the B-alanine list sodium salt of 18%; The isopropyl alcohol of 3%; The C9-11 Pareth-3 of 3%; The PEG-2 cocoyl methyl ammonium chloride of 2%; The isomery ten alcohol polyoxyethylene ether of 2%; 4,4-two (2-sulfonated styrene) biphenyl disodium salts of 13%; The Nano Silver of 3%; She's amount is deionized water.
The ethylene glycol phenyl ether of example 2:1%; N-(2-hydroxyethyl)-N-(2-ethylhexyl) the B-alanine list sodium salt of 20%; The isopropyl alcohol of 2%; The C9-11 Pareth-3 of 4%; The PEG-2 cocoyl methyl ammonium chloride of 4%; The isomery ten alcohol polyoxyethylene ether of 5%; 4,4-two (2-sulfonated styrene) biphenyl disodium salts of 10%; The Nano Silver of 5%; Surplus is deionized water.
Wherein, ethylene glycol phenyl ether (CASnumber-122-99-6) can be used as water paint and ink coalescents, spices fixastive, the smooth agent of ink, medicinal anticorrosion and bactericidal agent, electronic cleaning agent and ink drying retardant etc.
N-(2-hydroxyethyl)-N-(2-ethylhexyl) B-alanine list sodium salt (CASnumber-94441-92-6) is as the amphoteric surfactant of low foaming.Be mainly used in pressure washer, automobile washing machinery, furniture decoration cleans, low bubble carpet detergent and metal cleaning.
Isopropyl alcohol (CASnumber-67-63-0).
C9-11 Pareth-3 (CASnumber-68439-46-3) is as non-ionic surfactant.
PEG-2 cocoyl methyl ammonium chloride (CASnumber-61791-10-4) is as cationic surfactant.
Isomery ten alcohol polyoxyethylene ether (CASnumber-160875-66-1) purposes is polymkeric substance.
4,4-two (2-sulfonated styrene) biphenyl disodium salt (CASnumber-27344-41-8) is used on fluorescer.
Nano Silver (CASnumber-7440-22-4) is intended to bacteria growing inhibiting.
Component used by this fluorescent reagent is lower on cost, determines its use cost also comparatively cheap, is convenient to use on a large scale and promote.
The foregoing is only the preferred embodiment of the present invention, protection scope of the present invention is not limited in above-mentioned embodiment, and every technical scheme belonging to the principle of the invention all belongs to protection scope of the present invention.For a person skilled in the art, the some improvement carried out under the prerequisite not departing from principle of the present invention, these improvement also should be considered as protection scope of the present invention.
Claims (4)
1. environmental health ultraviolet fluorescent mark detection method, is characterized in that, the method comprises the steps:
S1., before to clean environment, fluorescent reagent is marked on environmental objects surface;
S2., after having cleaned, UV detection fluorescent reagent is utilized to mark whether to be eliminated;
If fluorescent reagent mark is eliminated, then cleaning is qualified, judges that environmental health is qualified;
If fluorescent reagent mark is not eliminated or part is eliminated, then cleaning is defective, judges that environmental health is defective;
Wherein, described fluorescent reagent is made up of the component of following weight percents:
2. environmental health ultraviolet fluorescent mark detection method according to claim 1, it is characterized in that, described fluorescent reagent is made up of the component of following weight percents:
3. environmental health ultraviolet fluorescent mark detection method according to claim 1, it is characterized in that, described fluorescent reagent is made up of the component of following weight percents:
4. according to the application of the arbitrary described environmental health ultraviolet fluorescent mark detection method of claim 1-3, it is characterized in that, the application of this environmental health ultraviolet fluorescent mark detection method applies to medical environment detection, laboratory environment detects, hotel environment detects, wherein, detect object and comprise microorganism, organism, inorganics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410202946.6A CN105092545A (en) | 2014-05-14 | 2014-05-14 | UV-fluorescent label detection method for environment sanitation and uses |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410202946.6A CN105092545A (en) | 2014-05-14 | 2014-05-14 | UV-fluorescent label detection method for environment sanitation and uses |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105092545A true CN105092545A (en) | 2015-11-25 |
Family
ID=54573461
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410202946.6A Pending CN105092545A (en) | 2014-05-14 | 2014-05-14 | UV-fluorescent label detection method for environment sanitation and uses |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105092545A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961541A (en) * | 2020-08-24 | 2020-11-20 | 上海斯瑞文特种油品科技有限公司 | Environment-friendly metal surface cleaning agent and preparation method thereof |
| CN114486837A (en) * | 2022-02-15 | 2022-05-13 | 清华大学 | Environmental surface contact propagation risk identification method, device, equipment and storage medium |
-
2014
- 2014-05-14 CN CN201410202946.6A patent/CN105092545A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961541A (en) * | 2020-08-24 | 2020-11-20 | 上海斯瑞文特种油品科技有限公司 | Environment-friendly metal surface cleaning agent and preparation method thereof |
| CN114486837A (en) * | 2022-02-15 | 2022-05-13 | 清华大学 | Environmental surface contact propagation risk identification method, device, equipment and storage medium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10994041B2 (en) | Use of fluorescent polymers in marking compositions for the diagnostic determination of cleaning performance | |
| KR101219829B1 (en) | Detection of microbe contamination on elastomeric articles | |
| Ferreira et al. | Condition of cleanliness of surfaces close to patients in an intensive care unit | |
| US20120085931A1 (en) | Fluorescing gel formulations and their applications | |
| CN104306176B (en) | A kind of quaternary anti-bacterial hand sanitizer | |
| Verran et al. | A critical evaluation of sampling methods used for assessing microorganisms on surfaces | |
| CN105092545A (en) | UV-fluorescent label detection method for environment sanitation and uses | |
| CN102586395A (en) | Plating medium-based leather material bacteriostasis effect test method | |
| CN104313118A (en) | Antibacterial efficacy detection method of diatom antibacterial material | |
| US20140366918A1 (en) | Compositions and methods for cleaning management | |
| CN205653445U (en) | Can be to real -time microbiological incubator who surveys of microorganism | |
| Loukili et al. | Effectiveness of detergent–disinfecting agents on Escherichia coli 54127 biofilm | |
| EP2634260B1 (en) | Biofilm-marking composition and method for detection of same on surfaces | |
| JP6451956B2 (en) | Antibacterial test liquid, antibacterial test liquid adhesion device, and antibacterial test method | |
| CN109645029A (en) | A kind of preparation and application of historical relic mould mould inhibitor | |
| CN110272973A (en) | ATP bioluminescence lgCB-lgIBThe method that calibration curve method detects antibiotic plastic bacteria resistance energy | |
| CN107960429A (en) | Disposable tableware disinfectant and preparation method thereof | |
| WO2024024648A1 (en) | Organic pigment composition, and spray agent comprising same | |
| CN205874374U (en) | Little biological specimens preparation platform of clinical laboratory bacterium | |
| Sutton | Microbial surface monitoring | |
| CN2108101U (en) | Culture bottle for testing disinfection effect of disinfectant | |
| CN204939480U (en) | A kind of food pathogenic microorganisms detection chip | |
| US20100112611A1 (en) | Procedure for detecting microbial contamination by bioluminescence in associative acrylic thickeners and products containing them | |
| NZ728055B2 (en) | Use of fluorescent polymers in marking compositions for the diagnostic determination of cleaning performance | |
| JP2006136299A (en) | Adhesive sheet for examining microorganism, method for producing the same, and method and kit for examining the microorganism by using the adhesive sheet |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151125 |