CN109645029A - A kind of preparation and application of historical relic mould mould inhibitor - Google Patents
A kind of preparation and application of historical relic mould mould inhibitor Download PDFInfo
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- CN109645029A CN109645029A CN201811189089.5A CN201811189089A CN109645029A CN 109645029 A CN109645029 A CN 109645029A CN 201811189089 A CN201811189089 A CN 201811189089A CN 109645029 A CN109645029 A CN 109645029A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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Abstract
It is a kind of historical relic mould mould inhibitor for sociales mould mould on historical relic and aspergillus niger design the invention belongs to biotechnology fungicide field.After No. 61 bacterium activation fermentations of bacillus subtilis of this laboratory preservation, the sterile supernatant that fermentation liquid obtains after processing is the mould inhibitor stoste of the active constituent containing lipopeptid.Bacteriostatic test plate is carried out for penicillium chrysogenum and aspergillus niger with mould inhibitor stoste and serial dilutions respectively, chooses paper, silk, leather, bamboo as material, the historical relic for simulating unlike material carries out simulation and presses down mould experiment.As a result, it has been found that all overgrowing with penicillium chrysogenum or aspergillus niger on the material of control group (sterile water), almost without fungus growth on the material of experimental group, 5 times of dilution (concentration of lipopeptid substance is 3-5 μ g/ml) mould effects of suppression are most obvious.Mould inhibitor carries out the practical mould experiment of suppression in Chinese Museum, has and presses down mould effect well.Mould inhibitor is placed three months in room temperature or 4 DEG C, and activity is unaffected.
Description
Technical field
The invention belongs to biotechnology fungicide field, it is related to common Superior fungus such as mould and song on a kind of inhibition historical relic
The preparation and application field of the mould inhibitor of mould growth, this formula are obtained using lipopeptid substance as the mould inhibitor pair of active constituent
Easily growing organic material such as papery, silk goods, leather and fur products, bamboo and wood of mould has good fungistatic effect；For breeding
The above-mentioned historical relic of mould, is handled by mould inhibitor, can effectively press down mycostatic growth.
Background technique
1, the meaning of historical relic mould bacteriostatic agent is designed:
The history culture in China is of long standing and well established, the rare cultural relics of succession its number not to the utmost, but organic matter historical relic is invaded vulnerable to mould
It attacks.Mould is various to the destruction of historical relic: mould utilizes the nutrient in organic matter during the growth process, causes to historical relic
It is direct to destroy；The destruction that heat accelerates historical relic is discharged in mould metabolic process；It is bonded in the growth course of mould organic
Matter, the pigment for making coloration of substrates can be generated by forming bacterial plaque or even some moulds；The metabolite of mould such as enzyme and other productions
Object can cause historical relic directly to destroy, and such as generate the organic acid for having corrosiveness to historical relic.In addition, ancient books repair and tide
House furniture under the conditions of wet is also required to press down mycostatic growth by mould threat etc..Therefore, design and develop it is effective,
Environmentally protective mould fungicide has to be of great significance in practice.
2, mould inhibitor and its existing defect are commonly used
(1) traditional inorganic fungicid: the inorganic fungicid of chemicals etc is widely applied mould inhibitor instantly.It is inorganic
Mould inhibitor usually has many advantages, such as that heat-resist, duration is good, antibacterial range is wide.But chemical agent itself is toxic and corrodes
Property, mould proof by the bacteriostasis of silver ion such as thymol LAg002 and LAg003, there are also some mould inhibitors by inorganic salts sulphur
Sour copper, mercury chloride, sodium fluoride etc. are made.The common feature of these mould inhibitors is that toxicity is big, has very big prestige to human body and environment
The side of body.
(2) novel low-toxicity mould inhibitor: long-acting anti-mildew spirit of NMF-1 mould inhibitor, geraniol etc., although they ensure that low toxicity,
Even nontoxic characteristic, but theirs is main mould proof at oily matter is belonged to, can not be water-soluble, application method is single, has relatively strong
Volatility, the mould proof time is shorter, needs timing to supplement, prepares complex.
(3) organic mould inhibitor: generally mainly by phenols (such as phenol), chlorophenols (such as pentachlorophenol), organic mercury salt (such as oleic acid
Phenyl mercury), organic copper salt (such as copper 8-quinolinolate), organic tin salt (such as three second of chlorination or tributyl tin) be made.Feature
It is to have certain toxicity, has certain harm to people, while there is corrosiveness to such as leather of the historical relic containing protein component etc..
3, historical relic except mould measure and there are the problem of
Historical relic's protection at present pollutes source mainly by control, applies to the overall situation, local environment, microenvironment of preservation low
Temperature is protected from light and reduces the measures such as artificial exogenous pollution.The measure that historical relic for there is mould growth is taken is main
It is to be cleaned first to the mould of growth, then has inorganic mould proof chemicals to be handled with some.But it is such
Method has very big drawback, because the toxicity of chemical agent itself, corrosivity etc. can all bring harm, even with accurate instrument
Device, which cleans historical relic, can also injure cultural artifact surface.The some ancient times stone buildings in Scotland once used chemical substance to clean
Moss, but these mosses " stage a comeback " again after several years.Therefore, environmentally protective and non-toxic efficient mould inhibitor research is very
It is necessary.The toxicity and corrosivity of common mould inhibitor are harmful to historical relic, human health and environment.It is therefore desirable to study nothing
Poison, the historical relic mould inhibitor inexpensive, not volatile, antimicrobial spectrum is wide, usage mode is various.
4, the antifungic action of lipopeptid substance:
Lipopeptid substance is mainly the biosurfactant that bacterium generates in metabolism.It is by peptide chain and fatty acid group
At a kind of amphiphilic species.It mycoplasma, desinsection, it is antitumor, antiviral and in terms of play huge work
With.Mechanism of action be it is various, including causing the change of membrane structure or damage, Cell wall synthesis, inducing cell being inhibited to wither
Die, inhibit protein, DNA, RNA synthesis etc..Show that the lipopeptid substance that bacillus marinus generates can if any scholar's research
To destroy the spore of fungi and the mycelia of excessive dissolution multicore.There is scholar's research to show that antibacterial lipopeptid is thin to mammal simultaneously
Born of the same parents are without mutagenicity or potential genetoxic.Life is made in the bacteriogenic fermentation liquid containing lipopeptid substance by the present invention
Object mould inhibitor has the features such as nontoxic, inexpensive, not volatile, antimicrobial spectrum is wide, usage mode is various.
Summary of the invention:
The present invention utilizes the fermentation liquid of bacillus subtilis, includes the lipopeptid class object for having strong bacteriostatic activity to mould
Matter prepares biological mildew inhibitor, has efficient, nontoxic and environmentally friendly characteristic.
1. bacterium bacterial strain: used in the present invention is the bacterium bacterial strain (No. 61) that the screening of this research department obtains, by 16S
The analysis of rDNA sequence belongs to bacillus subtilis (Bacillus subtilis), which protected on August 15th, 2018
China General Microbiological Culture Collection Center is ensconced, the biomaterial for proprietary program saves, address: Chaoyang District, Beijing City
The institute 3 of North Star West Road 1, Institute of Microorganism, Academia Sinica, deposit number are CGMCC No.16222, and bacterial strain metabolism produces
Contain lipopeptid substance in object, has very strong fungistatic effect to moulds such as mould and aspergillus nigers.
2. optimal conditions of fermentation: No. 61 bacterium being transferred on LB plate, 37 DEG C of cultures are for 24 hours；Take a collarium big with collarium is connect
The colony inoculation of small (about diameter 4mm) is in the 250ml triangular flask containing 100mlLB fluid nutrient medium, 37 DEG C, 250rpm training
Support and be used as seed liquor for 24 hours, bacterium solution is then taken to be inoculated into Landy fermentation medium, inoculum concentration 9%, after inoculation in 37 DEG C,
250rpm cultivates 32-48h, fermentation liquid is centrifuged (12000rpm, 10min) after culture, supernatant passes through bacteriological filter again
Device obtains the without fermented liquid containing lipopeptid.
Landy culture medium prescription: into 950mL distilled water be added glucose 20g, Pidolidone 5g, KH2PO41g,
MgSO47H2O 0.5g, KCl 0.5g, yeast powder 1g, 2 × 10-3g of L-phenylalanine, MnSO45 × 10-3g, CuSO4
0.16 × 10-3g of 5H2O, 0.15 × 10-3g of FeSO47H2O adjust pH value to 7.0 after solute dissolution, with distilled water mend to
1000mL, 115 DEG C of high pressure sterilization 20min.
3. minimal inhibitory concentration: fermentation liquid is diluted to 5 times, 10 times and 15 times respectively, respectively with fermentation liquid stoste, 5 times it is dilute
Release penicillium chrysogenum and aspergillus niger progress bacteriostatic test plate that liquid, 10 times of dilutions and 15 times of dilutions are saved for laboratory.
Specific method is the spore suspension (concentration 10 for taking 0.2ml penicillium chrysogenum and aspergillus niger respectively6-107/ m) coating PDA plate,
Fermentation liquid stoste and dilution are dipped respectively with the aseptic filter paper piece that diameter is 0.5 centimetre after 28 DEG C of culture 6hr, are attached to PDA
On plate, continue to observe in 28 DEG C of culture 12-24hr and measure inhibition zone size.As a result, it has been found that diluting 5 times of fermentation liquid (rouge
The concentration of peptide matters is 3-5 μ g/ml) there is optimal fungistatic effect, diluting 10 times of fermentation liquids, (lipopeptid concentration is 1.2-2.5
μ g/ml) still there is bacteriostatic activity, it dilutes 15 times of fermentation liquids and loses bacteriostatic activity.Therefore fermentation liquid minimum inhibitory concentration is dilution
10 times of fermentation liquids, lipopeptid class content of material are 1.2-2.5 μ g/ml.
4. the detection of lipopeptid substance and concentration mensuration
A certain amount of fermentation liquid is taken, pH is transferred to 7.5-9.0 with NaOH solution, 10000rpm is centrifuged 15min, takes supernatant, uses
The pH of supernatant is transferred to 2.0 by HCl, with the CHCl of same volume3/CH3OH (V/V.3/1) continuous extraction supernatant 5 times, merge extraction
Phase, concentration, washs 5 times, then evaporation under reduced pressure removed organic phase with the HCL aqueous solution of pH 2.0, obtained sample does amino acid
Analysis, to calculate lipopeptid content, lipopeptid content is calculated as follows, and wherein C is lipopeptid concentration, mol.L-1；V is fermented liquid
Product, L；M is the quality of glutamic acid, g；M is the molecular weight of paddy amino acid: C=m/ (V × M), mol.L-1
TLC analysis is carried out with the sample simultaneously.Sample is dissolved in CH2Cl2, thin plate chromatography (TLC), solvent CHCl3/
CH3COOH(V/V.8/2).It dries up, thin plate is put into closed container after expansion, heat 1h in 110, it is former using dense HCl
Position hydrolysis sprays ninhydrin and heats colour developing.
5. preservation condition: the fermentation liquid containing lipopeptid class antibacterial substance and dilution being stored in room temperature and 4 DEG C respectively, protected
Bacteriostatic activity is examined respectively after depositing different time.As a result, it has been found that being saved in third month under the conditions of both, it is minimum antibacterial dense
Degree is identical as the minimal inhibitory concentration of freshly prepd fermentation liquid, i.e., bacteriostasis in three months is saved under the conditions of both not to be had
Significant change.
6. in-kind simulation presses down mould experiment: choosing paper, silk, leather, bamboo as material, simulate the text of unlike material
Object carries out simulating the mould proof or mould experiment of suppression.Two parts of above-mentioned material are chosen respectively, and portion is control group, another is experimental group.?
Sterile water is sprayed on control group a variety of materials surface, sprays the fermentation liquid of different dilutions on experimental group a variety of materials surface.Sprinkling
After place 30min, then respectively spray concentration be 106-107The penicillium chrysogenum of/ml and the spore suspension of aspergillus niger, connect various
The material of bacterium is placed into the surface of PDA plate, provides the nutrition of penicillium chrysogenum and Aspergillus Niger Growth.Then in 28 DEG C of culture 1-3
It.Observe the growing state of penicillium chrysogenum and aspergillus niger on above-mentioned different materials.As a result, it has been found that paper, silk in control group
Silk fabric, leather all overgrow be inoculated with penicillium chrysogenum or aspergillus niger on bamboo.The fermentation liquid experimental group for spraying various concentration,
Paper, silk, leather, on bamboo almost without times dilution fermentation liquid of fungus growth, especially 5, press down mould effect and become apparent from.
7. historical relic presses down mould experiment
The African woodcarving historical relic of The National Museum of China preservation, have it is several it is different degrees of grown mould, we are first
Upper sterile water is dipped in absorbent cotton to remove the mould of growth, is then made of 5 times of dilutions of sterile supernatant of No. 61 fermented liquids
Mould inhibitor handled, i.e., dip in upper mould inhibitor with rayon balls and handle long bacterium position, processing is twice.As a result, it has been found that with biology
Treated that effect is very good for mould inhibitor, and 1 year and a half is gone over, and originally the position of long bacterium is still without long bacterium.
Detailed description of the invention:
Fig. 1 mould inhibitor is placed at room temperature for different time to the inhibiting effect of aspergillus niger, three months antibacterial effects of room temperature and 4 DEG C of preservations
Fruit is held essentially constant
Fig. 2 mould inhibitor is placed at room temperature for different time to the inhibiting effect of mould, three months fungistatic effects of room temperature and 4 DEG C of preservations
It is held essentially constant
The TLC of lipopeptid class is detected in Fig. 3 mould inhibitor, and dark parts are lipopeptid substance in ellipse
For Fig. 4 mould inhibitor to the mould experiment of the suppression of paper, a left side is control, in inhibit mould, the right side is inhibits aspergillus niger (control group
Sterile water is first sprayed, mycotic spore is then inoculated with；Experimental group first sprays mould inhibitor, then sprays mycotic spore)
For Fig. 5 mould inhibitor to the mould experiment of the suppression of silk, a left side is control, in inhibit mould, the right side is inhibits aspergillus niger (control group
Sterile water is first sprayed, mycotic spore is then inoculated with, observes result after culture；Experimental group first sprays mould inhibitor, then sprays mould
Spore observes result after culture)
For Fig. 6 mould inhibitor to the mould experiment of the suppression of leather, a left side is control, in be that inhibit mould, the right side be inhibition aspergillus niger (control group
Sterile water is first sprayed, mycotic spore is then inoculated with, observes result after culture；Experimental group first sprays mould inhibitor, then sprays mould
Spore observes result after culture)
Fig. 7 mould inhibitor to the mould experiment of the suppression of bamboo cane, left control, in be that inhibit mould, the right side be to inhibit aspergillus niger (control group elder generation
Sterile water is sprayed, mycotic spore is then inoculated with, observes result after culture；Experimental group first sprays mould inhibitor, then sprays mould spore
Son observes result after culture)
Specific embodiment:
1. the preparation of fermentation condition and mould inhibitor
By in No. 61 bacterium streak inoculations to LB plate, 37 DEG C of cultures for 24 hours, choose single colonie, take a collarium bacterium with collarium is connect
(diameter about 4mm) is inoculated into the 250ml triangular flask containing 100ml LB liquid medium, 37 DEG C, 250rpm culture
It is used as seed liquor for 24 hours.Seed liquor is 5 × 10 containing bacteria concentration6-6×107, OD560For 0.4-0.6.Seed liquor 9ml is taken to be inoculated into
In 250ml triangular flask, it is provided with the Landy culture medium of 100ml, cultivates 32-48h in 37 DEG C, 250rpm.It will after culture
Fermentation liquid is centrifuged the biofilter that supernatant is 0.24 μm using filter sizes in 4 DEG C of centrifugations (12000rpm, 10min),
Bacteria-free filtrate is the without fermented liquid stoste containing lipopeptid substance, and stoste is saved backup in 4 DEG C.
2. fermentation liquid is to the bacteriostatic test plate of penicillium chrysogenum and aspergillus niger
The without fermented liquid of preservation is diluted 5 times, 10 times and 15 times respectively, measures the concentration of lipopeptid substance respectively.Point
It Yong not the penicillium chrysogenum that saves for laboratory of fermentation liquid stoste, 5 times of dilutions, 10 times of dilutions and 15 times of dilutions and black
Aspergillus carries out bacteriostatic test plate.Specific method is to take the spore suspension of 0.2ml penicillium chrysogenum and aspergillus niger respectively (concentration is
106-107/ m) coating PDA plate, fermentation is dipped respectively with the aseptic filter paper piece that diameter is 0.5 centimetre after 28 DEG C of culture 6hr
Liquid stoste and dilution, are attached on PDA plate, continue in 28 DEG C of culture 12-24hr, observe and measure inhibition zone size (Fig. 1,
Fig. 2).As a result, it has been found that diluting 5 times of fermentation liquids (concentration of lipopeptid substance is 3-5 μ g/ml) has optimal fungistatic effect, it is dilute
Releasing 10 times of fermentation liquids (lipopeptid concentration is 1.2-2.5 μ g/ml) still has bacteriostatic activity, dilutes 15 times of fermentation liquids and loses antibacterial work
Property.Fermentation liquid minimum inhibitory concentration is 10 times of fermentation liquids of dilution, and lipopeptid class content of material is 1.2-2.5 μ g/ml.
3. the detection of lipopeptid substance and concentration mensuration in mould inhibitor
A certain amount of fermentation liquid is taken, pH is transferred to 7.5-9.0 with NaOH solution, 10000rpm is centrifuged 15min, takes supernatant, uses
The pH of supernatant is transferred to 2.0 by HCl, with the CHCl of same volume3/CH3OH (V/V.3/1) continuous extraction supernatant 5 times, merge extraction
Phase, concentration, washs 5 times, then evaporation under reduced pressure removed organic phase with the HCL aqueous solution of pH 2.0, obtained sample does amino acid
Analysis, to calculate lipopeptid content, lipopeptid content is calculated as follows, and wherein C is lipopeptid concentration, mol.L-1；V is fermented liquid
Product, L；M is the quality of glutamic acid, g；M is the molecular weight of paddy amino acid: C=m/ (V × M), mol.L-1
TLC analysis is carried out with the sample simultaneously.Sample is dissolved in CH2Cl2, thin plate chromatography (TLC), solvent CHCl3/
CH3COOH(V/V.8/2).It dries up, thin plate is put into closed container after expansion, heat 1h in 110, it is former using dense HCl
Position hydrolysis sprays ninhydrin and heats colour developing.The Activity determination of lipopeptid substance is shown in attached drawing 3 in fermentation liquid, lipopeptid substance
Concentration is 16.25-26.30 μ g/ml
4. material object suppression mould simulated experiment
Paper, silk, leather, bamboo are chosen as material, the historical relic for simulating unlike material carries out simulating mould proof or press down mould
Experiment.Two parts of above-mentioned material are chosen respectively, and portion is control group, another is experimental group.It is sprayed on control group a variety of materials surface
Spill sterile water, dosage 1ml/2.5cm2, without fermented liquid stoste and different dilutions are sprayed on experimental group a variety of materials surface
Fermentation liquid, dosage 1ml/2.5cm2.In being placed at room temperature for 30min after sprinkling, then spray concentration is 10 respectively6-107/ ml's
The spore suspension of penicillium chrysogenum and aspergillus niger, dosage 1ml/10cm2, a variety of materials of control group and experimental group are placed in
In the big culture dish that one diameter is 20cm, it is put into the rayon balls for dipping in water, covers ware lid, cultivated 1-3 days in 28 DEG C.
Observe the growing state of penicillium chrysogenum and aspergillus niger on above-mentioned different materials.As a result, it has been found that the paper of control group, silk,
Be inoculated with penicillium chrysogenum or aspergillus niger are all overgrowed on leather, bamboo.The fermentation liquid experimental group for spraying various concentration, in paper
, on silk, leather, bamboo almost without fungus growth, mould effect (Fig. 4-figure the most significant is pressed down after diluting 5 times with distilled water
7)。
Claims (5)
1. a kind of historical relic mould mould inhibitor, which is characterized in that by No. 61 hairs of bacillus subtilis (Bacillus subtilis)
Zymotic fluid is prepared, and the bacillus subtilis No. 61 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center
Deposit number are as follows: CGMCC No.16222.
2. historical relic mould mould inhibitor as described in claim 1, which is characterized in that effective component is bacillus subtilis fermentation liquor
In lipopeptid substance.
3. the preparation and application of historical relic mould mould inhibitor as described in claim 1, which is characterized in that utilize withered grass gemma
Bacillus No. 61 are passed through seed tank culture and fermented and cultured, obtain bacillus subtilis fermentation liquor, the bacillus subtilis fermentation liquor
And solution is applied to cultural artifact surface progress control of mold after dilution.
4. the purposes of historical relic mould mould inhibitor as described in claim 1, which is characterized in that in paper, silk goods, leather system
The prevention and treatment of mould in product, bamboo product, woodwork, cultural relics in the collection of cultural institution or building.
5. the purposes of historical relic mould mould inhibitor as claimed in claim 1 or 5, which is characterized in that the mould includes to produce yellow blueness
Mould, aspergillus niger.
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| CN201811189089.5A CN109645029A (en) | 2018-10-12 | 2018-10-12 | A kind of preparation and application of historical relic mould mould inhibitor |
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| CN201811189089.5A CN109645029A (en) | 2018-10-12 | 2018-10-12 | A kind of preparation and application of historical relic mould mould inhibitor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114766516A (en) * | 2022-04-20 | 2022-07-22 | 四川大学 | Lipopeptide compound bacteriostatic agent suitable for trichoderma and penicillium on sclerotin cultural relics, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1477189A (en) * | 2003-07-10 | 2004-02-25 | 南京农业大学 | Strain for food preservation and its anti-bacterial product |
| CN103355356A (en) * | 2013-01-28 | 2013-10-23 | 大连三仪动物药品有限公司 | Application of fermentation liquor of bacillus subtilis in inhibition of growth of penicillium and aspergillus niger |
| CN106635929A (en) * | 2017-02-14 | 2017-05-10 | 河南工业大学 | Bacillus subtilis strain and application of antifungal metabolite in grain storage |
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2018
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1477189A (en) * | 2003-07-10 | 2004-02-25 | 南京农业大学 | Strain for food preservation and its anti-bacterial product |
| CN103355356A (en) * | 2013-01-28 | 2013-10-23 | 大连三仪动物药品有限公司 | Application of fermentation liquor of bacillus subtilis in inhibition of growth of penicillium and aspergillus niger |
| CN106635929A (en) * | 2017-02-14 | 2017-05-10 | 河南工业大学 | Bacillus subtilis strain and application of antifungal metabolite in grain storage |
Non-Patent Citations (1)
| Title |
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| 陆金荣 等: ""枯草芽孢杆菌 GD 产脂肽的抗菌作用及活性成分分析"", 《畜牧与兽》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114766516A (en) * | 2022-04-20 | 2022-07-22 | 四川大学 | Lipopeptide compound bacteriostatic agent suitable for trichoderma and penicillium on sclerotin cultural relics, and preparation method and application thereof |
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Application publication date: 20190419 |