CN105087134A - Sunflower seed oil aqueous enzymatic extraction method - Google Patents
Sunflower seed oil aqueous enzymatic extraction method Download PDFInfo
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- CN105087134A CN105087134A CN201510460881.XA CN201510460881A CN105087134A CN 105087134 A CN105087134 A CN 105087134A CN 201510460881 A CN201510460881 A CN 201510460881A CN 105087134 A CN105087134 A CN 105087134A
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Abstract
The invention provides a sunflower seed oil aqueous enzymatic extraction method. The extraction process includes the steps of sunflower seed uncoating, drying, smashing and grinding, soaking, compound enzyme treatment, three-phase separation, oil phase filtering, deacidification, dehydration and finished product sunflower seed oil. Compound enzyme is used to conduct extraction on sunflower seed processing mixed liquor, the free oil yield is high, the processing condition is gentle, and the extraction method is environmentally friendly.
Description
Technical field
The present invention relates to vegetable oil extraction field, particularly relate to a kind of sunflower seed oil aqueous enzymatic extraction technique.
Background technology
Trisun Oil R 80 is with the famous health-care edible oil of the linolic acid of high-content.According to State Bureau of Grain Reserve's Xi'an grease studying and designing institute professor Cao Wanxin introduction, in 21 century, cardiovascular and cerebrovascular diseases such as the diseases such as coronary heart disease, cerebral apoplexy, cerebral thrombosis, arteriosclerosis, hypertension increase, and long-term edible suitable oil and fat product, can have greatly improved to above-mentioned symptom.Trisun Oil R 80 is exactly one very outstanding in healthy grease, and sunflower seed oil has become the first-selection oil of human consumer and cook, and sunflower seed oil consumption worldwide ranks the 3rd in all vegetables oil.Sunflower seed oil contains the multiple materials useful to the mankind such as sterol, VITAMIN, linolic acid, and wherein natural tocopherol level content in all main plant oil is the highest; And linoleic acid content can reach about 70%.Sunflower seed oil can reduce cholesterol in serum level, reduces triglyceride levels, has the effect reduced blood pressure.Further, sunflower seed oil light transparent, culinary art time can retain whole food local flavor, its smoke point is also very high, can exempt the harm of oil smoke to human body.
Aqueous enzymatic method technique is on the basis of Mechanical Crushing, adopt the enzyme of energy degrading plant oil plant cell walls, or the complex body such as ester gp, polysaccharide ester is had to the enzyme of Degradation, comprise cellulase, polygalacturonase, amylase, proteolytic enzyme etc., act on oilseed, make grease be easy to discharge from oil plant, utilize non-oil component to oil and the difference of water avidity, utilize profit proportion different and by oil and non-oil component separating simultaneously.In aqueous enzymatic method technique, enzyme is except oilseed cell of degrading, complex body such as decomposition ester gp, polysaccharide ester etc., the ester gp film being wrapped in oil droplets that oilseed is formed in defibrination process can also be destroyed, reduce the stability of milk sap, thus improve the yield of free oil.
Through the retrieval to existing document, found that patent discloses a kind of aqueous enzymatic extraction sunflower seed oil and reclaiming method of protein of application number 200610096926.0, this invention with the sunflower seeds that shells for raw material, after dry ground, add citrate buffer, enzyme digestion reaction is carried out with complex cellulase, centrifugal after enzyme digestion reaction, obtain free oil, milk sap, hydrolyzed solution and grouts, the milk sap obtained is through high speed centrifugation, to reentry free oil, hydrolyzed solution after filtration, vacuum concentration, alcohol precipitation, centrifugal, precipitation and lyophilize are reclaimed and are obtained protein.But the document only employs complex cellulase carries out aqueous enzymatic extraction, do not add other biological enzyme, destruction albumen that can not be corresponding, the materials such as starch.Application number 201310320934.9 the present invention relates to a kind of aqueous enzymatic extraction rapeseed oil and reclaims method of protein.After being crushed by raw material vegetable seed, acid liquid high temperature (80-120 DEG C) soaks 0.1-2h, defibrination, is separated and removes cot.Filtrate at 30-60 DEG C of enzymolysis 2-6h, then adds proteolytic enzyme and the prozyme 30-60 DEG C of enzymolysis 2-6h thereof of 100-500U/g with 50-500U/g complex cellulase, and centrifugation, residue can make feed by convection drying.
Summary of the invention
Provide hereinafter about brief overview of the present invention, to provide about the basic comprehension in some of the present invention.Should be appreciated that this general introduction is not summarize about exhaustive of the present invention.It is not that intention determines key of the present invention or integral part, and nor is it intended to limit the scope of the present invention.Its object is only provide some concept in simplified form, in this, as the preorder in greater detail discussed after a while.
In order to overcome the above problems, the invention provides a kind of sunflower seed oil aqueous enzymatic extraction technique, described extraction process is: shelling sunflower seeds → drying → pulverizing, grinding → immersion → prozyme process → three phase separation → oil phase filtration → depickling → dehydration → finished product sunflower seed oil.
Preferably, described immersion process soaks 0.1-2h for adding acidic solution high temperature.
Preferably, the pH of described acidic solution is 4-6.
Preferably, the soaking temperature of described acidic solution is 70-95 DEG C.
Preferably, the prozyme that described prozyme process is selected is cellulase, amylase, proteolytic enzyme, zytase.
Preferably, the temperature of described prozyme process is 30-60 DEG C.
Preferably, the time of described prozyme process is 3-8h.
Preferably, the complete processing after the aqueous phase after described three phase separation is aqueous phase → concentrated → spraying dry → sunflower protein powder.
Preferably, the slag phase complete processing after described three phase separation is slag phase → warm air drying → sunflower protein fiber powder.
The present invention has following beneficial effect:
Method provided by the invention uses prozyme to extract sunflower seeds processing mixed solution, free oil yield is higher, and processing conditions is gentle, environmental protection, and can also utilize mutually aqueous phase and slag in leaching process, obtain multiple valuable by product.
Embodiment
Embodiments of the invention are described below.The element described in one embodiment of the invention and feature can combine with the element shown in one or more other embodiment and feature.It should be noted that for purposes of clarity, in explanation, eliminate expression and the description of unrelated to the invention, parts known to persons of ordinary skill in the art and process.
Embodiment 1
1) prozyme treating processes
Adding vinegar acid for adjusting pH after shelling sunflower seeds conventionally being carried out dry, pulverize grinding is 4.5, and after soaking 1h at 95 DEG C, add 300U/g cellulase, 200U/g amylase, 450U/g proteolytic enzyme, 300U/g zytase processes 3h at 40 DEG C.
2) three phase separation process
After prozyme process, mixed solution is carried out centrifugation, obtains oil phase, aqueous phase and slag phase, then carry out following operation
Oil phase → filtration → depickling → dehydration → processed oil
Aqueous phase → concentrated → spraying dry → sunflower protein powder
Slag phase → warm air drying → sunflower protein fiber powder
Result can obtain one-level sunflower seed oil.
Embodiment 2
1) prozyme treating processes
Adding phosphorus acid for adjusting pH after shelling sunflower seeds conventionally being carried out dry, pulverize grinding is 5, and after soaking 1h at 80 DEG C, add 300U/g cellulase, 200U/g amylase, 450U/g proteolytic enzyme, 300U/g zytase processes 4h at 50 DEG C.
2) three phase separation process
After prozyme process, mixed solution is carried out centrifugation, obtains oil phase, aqueous phase and slag phase, then carry out following operation
Oil phase → filtration → depickling → dehydration → processed oil
Aqueous phase → concentrated → spraying dry → sunflower protein powder
Slag phase → warm air drying → sunflower protein fiber powder
Result can obtain one-level sunflower seed oil.
Embodiment 3
1) prozyme treating processes
Adding vinegar acid for adjusting pH after shelling sunflower seeds conventionally being carried out dry, pulverize grinding is 4, and after soaking 1h at 70 DEG C, add 300U/g cellulase, 200U/g amylase, 450U/g proteolytic enzyme, 300U/g zytase processes 8h at 30 DEG C.
2) three phase separation process
After prozyme process, mixed solution is carried out centrifugation, obtains oil phase, aqueous phase and slag phase, then carry out following operation
Oil phase → filtration → depickling → dehydration → processed oil
Aqueous phase → concentrated → spraying dry → sunflower protein powder
Slag phase → warm air drying → sunflower protein fiber powder
Result can obtain one-level sunflower seed oil.
Although last it is noted that described the present invention and advantage thereof in detail above, be to be understood that and can carry out various change when not exceeding the spirit and scope of the present invention limited by appended claim, substituting and converting.And scope of the present invention is not limited only to the specific embodiment of process, equipment, means, method and step described by specification sheets.One of ordinary skilled in the art will readily appreciate that from disclosure of the present invention, can use perform the function substantially identical with corresponding embodiment described herein or obtain and its substantially identical result, existing and that will be developed in the future process, equipment, means, method or step according to the present invention.Therefore, appended claim is intended to comprise such process, equipment, means, method or step in their scope.
Claims (9)
1. a sunflower seed oil aqueous enzymatic extraction technique, is characterized in that, described extraction process is: shelling sunflower seeds → drying → pulverizing, grinding → immersion → prozyme process → three phase separation → oil phase filtration → depickling → dehydration → finished product sunflower seed oil.
2. sunflower seed oil aqueous enzymatic extraction technique according to claim 1, is characterized in that, described immersion process soaks 0.1-2h for adding acidic solution high temperature.
3. sunflower seed oil aqueous enzymatic extraction technique according to claim 2, it is characterized in that, the pH of described acidic solution is 4-6.
4. sunflower seed oil aqueous enzymatic extraction technique according to claim 2, it is characterized in that, the soaking temperature of described acidic solution is 70-95 DEG C.
5. sunflower seed oil aqueous enzymatic extraction technique according to claim 1, is characterized in that, the prozyme that described prozyme process is selected is cellulase, amylase, proteolytic enzyme, zytase.
6. sunflower seed oil aqueous enzymatic extraction technique according to claim 1, is characterized in that, the temperature of described prozyme process is 30-60 DEG C.
7. sunflower seed oil aqueous enzymatic extraction technique according to claim 1, is characterized in that, the time of described prozyme process is 3-8h.
8. sunflower seed oil aqueous enzymatic extraction technique according to claim 1, is characterized in that, the complete processing of the aqueous phase after described three phase separation is aqueous phase → concentrated → spraying dry → sunflower protein powder.
9. sunflower seed oil aqueous enzymatic extraction technique according to claim 1, is characterized in that, the slag phase complete processing after described three phase separation is slag phase → warm air drying → sunflower protein fiber powder.
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| CN201510460881.XA CN105087134A (en) | 2015-07-30 | 2015-07-30 | Sunflower seed oil aqueous enzymatic extraction method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109776275A (en) * | 2019-03-15 | 2019-05-21 | 黑龙江阳光工业大麻研究院 | A method of cannabidiol is extracted using microbial fermentation |
| CN109809969A (en) * | 2019-03-15 | 2019-05-28 | 黑龙江阳光工业大麻研究院 | A kind of method that enzyme process combination embrane method extracts cannabidiol |
| CN112574811A (en) * | 2020-12-15 | 2021-03-30 | 佳格食品(厦门)有限公司 | Moderate processing method of sunflower seed oil |
| CN113088376A (en) * | 2021-04-12 | 2021-07-09 | 武汉轻工大学 | Blend oil based on zanthoxylum seed oil and high oleic acid rapeseed oil and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1952094A (en) * | 2006-10-18 | 2007-04-25 | 江南大学 | Process for extracting sunflower seed oil and recovering protein by aqueous enzymatic method |
| CN104745290A (en) * | 2015-04-20 | 2015-07-01 | 江苏金洲粮油食品有限公司 | High-oil-yield sunflower seed oil extraction production process |
-
2015
- 2015-07-30 CN CN201510460881.XA patent/CN105087134A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1952094A (en) * | 2006-10-18 | 2007-04-25 | 江南大学 | Process for extracting sunflower seed oil and recovering protein by aqueous enzymatic method |
| CN104745290A (en) * | 2015-04-20 | 2015-07-01 | 江苏金洲粮油食品有限公司 | High-oil-yield sunflower seed oil extraction production process |
Non-Patent Citations (1)
| Title |
|---|
| 李爱科: "《中国蛋白质饲料资源》", 31 January 2013, 北京：中国农业大学出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109776275A (en) * | 2019-03-15 | 2019-05-21 | 黑龙江阳光工业大麻研究院 | A method of cannabidiol is extracted using microbial fermentation |
| CN109809969A (en) * | 2019-03-15 | 2019-05-28 | 黑龙江阳光工业大麻研究院 | A kind of method that enzyme process combination embrane method extracts cannabidiol |
| CN112574811A (en) * | 2020-12-15 | 2021-03-30 | 佳格食品(厦门)有限公司 | Moderate processing method of sunflower seed oil |
| CN113088376A (en) * | 2021-04-12 | 2021-07-09 | 武汉轻工大学 | Blend oil based on zanthoxylum seed oil and high oleic acid rapeseed oil and preparation method thereof |
| CN113088376B (en) * | 2021-04-12 | 2023-02-24 | 武汉轻工大学 | Blend oil based on zanthoxylum seed oil and high oleic acid rapeseed oil and preparation method thereof |
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