CN105077158A - Method for manufacturing cheese flavor milk essence bases - Google Patents

Method for manufacturing cheese flavor milk essence bases Download PDF

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Publication number
CN105077158A
CN105077158A CN201510521683.XA CN201510521683A CN105077158A CN 105077158 A CN105077158 A CN 105077158A CN 201510521683 A CN201510521683 A CN 201510521683A CN 105077158 A CN105077158 A CN 105077158A
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milk
parts
cheese
local flavor
liquid
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CN105077158B (en
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王婧婧
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Guangzhou Tianziyou Biotechnology Co ltd
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Abstract

The invention discloses a method for manufacturing cheese flavor milk essence bases. The method includes steps of fermenting whole-milk powder by the aid of lactic acid bacteria and rhizopus oryzae to obtain milk fermented broth; adding milk fat into the milk fermented broth and carrying out enzymatic hydrolysis on the milk fermented broth by the aid of lipase and protease to obtain milk enzymatic hydrolysis liquid; adding amino acid, reducing sugar and solvents into the milk enzymatic hydrolysis liquid, and enabling the amino acid, the reducing sugar, the solvents and the milk enzymatic hydrolysis liquid to carry out thermal reaction to obtain the cheese flavor milk essence bases. The method has the advantages that procedures for manufacturing the cheese flavor milk essence bases are natural, generated aroma is natural, harmonious and mellow, the cheese flavor milk essence bases have certain heat resistance and can be applied to milk beverage, baking food and home cooking food, and accordingly the method has a broad market prospect.

Description

A kind of preparation method of cheese's local flavor frankincense base
Technical field
The present invention relates to a kind of new method being prepared cheese's local flavor frankincense base by biotechnology and thermal response technology.
Background technology
Hot reaction essence is product prepared by a kind of heating raw materials added in food or reaction essence by raw-food material and (or) permission, the natural edible essence mainly prepared by simulated foods heating process generation fragrance.Thermal response is albumen, polypeptide, amino acid, fat and the plurality of raw materials such as sugared, complicated chemical reaction is produced in heating process, thus define distinctive local flavor, wherein most importantly Maillard reaction, be that food produces one of important channel of fragrance in process.
By changing the raw material type such as amino acid, fat and sugar and content, the fragrance producing different characteristic local flavor can be reacted, the investigation and application comparative maturity in saline taste, as by being hydrolyzed to chicken, then add sulfur-containing amino acid and reduced sugar, thermal response can obtain chicken essence (Chen Haitao, Xu Xiaolan, Zhang Ning, Sun Baoguo, Wang He, chicken enzymolysis process is on the impact [J] of model chicken flavour fragrance, Food Science, 2013(09)).The development and application of thermal response on sweet taste essence is also in attempts the development phase, and product ripe on market is less.The present invention passes through technological innovation, make up this block market demand, to be rich in the dairy products of albumen and fat for initial feed, by the catalytic action of biofermentation and enzyme preparation, strengthen fragrance fragrance, the amino acid simultaneously discharged, aliphatic acid etc., as the critical materials source needed for thermal response, improve characteristic fragrance further by thermal response, increase mouthfeel, the required perfume base of exploitation.
Summary of the invention
For the deficiencies in the prior art part, the object of the invention is to main with natural dairy products for raw material, fragrance is promoted by the metabolism of microorganism and the catalytic action of enzyme preparation, and the precursor needed for Heat of Formation reaction, be aided with and add amino acid and reduced sugar, utilize thermal response to prepare cheese's local flavor frankincense base.
For achieving the above object, the preparation method of a kind of cheese's local flavor frankincense base designed by the present invention, is characterized in that comprising the following steps:
A. milk fermentation liquid is prepared: get whole-fat milk powder 100 parts, add the citric acid-sodium citrate buffer of 200 ~ 800 parts of pH5.0 ~ 6.5, be uniformly mixed, be cooled to after room temperature through pasteurize, add Lactobacillus casei and Rhizopus oryzae, 30 ~ 45 DEG C of condition bottom fermentation 4 ~ 24h, obtain dairy fermentation liquid after pasteurize; Described Lactobacillus casei addition is every gram of full-cream emulsion 10 5-10 7cfu; The addition of described Rhizopus oryzae is every gram of full-cream emulsion 10 4-10 6individual spore;
B. newborn enzymolysis liquid is prepared: get milk fermentation liquid 100 parts, butter oil 5 ~ 25 parts, 0.1 ~ 1.5 part, lecithin, homogeneous liquid is obtained through homogeneous after shearing dispersion, add 0.01 ~ 0.05 part, lipase after pasteurize, proteinase-10 .03 ~ 0.15 part, carry out enzymolysis 0.5 ~ 8h in 30 ~ 55 DEG C, 95min goes out enzyme 15min, obtains newborn enzymolysis liquid;
C. thermal response cheese local flavor frankincense base is prepared: get newborn enzymolysis liquid 100 parts, add L-arginine 1.5 ~ 7.5 parts, L-PROLINE 1.5 ~ 7.5 parts, 1B 1.0 ~ 5.0 parts, L-Histidine 0.6 ~ 3.0 part, L-Aspartic acid 0.5 ~ 2.5 part, 5 '-flavour nucleotide disodium 0.6 ~ 3.0 part, reduced sugar 6.0 ~ 30.0 parts, solvent 200 ~ 1000 parts, adjustment pH to 6.5 ~ 7.5, are warming up to 95 ~ 120 DEG C of condensing reflux reaction 1 ~ 6h under stirring condition, be cooled to room temperature, after crossing colloid mill, obtain cheese's local flavor frankincense base.
Wherein, described butter oil is the mixture of one or more compositions in anhydrous butter oil, butter and whipping cream.
Described reduced sugar is the mixture of one or more compositions in glucose, fructose, wood sugar, arabinose, lactose, maltose, rhamnose and trehalose.
Described solvent is the mixture of one or more compositions in water, propane diols and glycerine.
Cheese's local flavor frankincense base prepared by the present invention has the following advantages:
(1) fermentation and enzymatic process natural, the fragrance of generation is coordinated naturally;
(2) by the fermentation of Rhizopus oryzae, produce abundant head fragrant, coordinate the fragrance of whole perfume base;
(3) produce abundant mouthfeel by thermal response, fragrance is mellow, and has certain heat resistance.
Detailed description of the invention
Below in conjunction with instantiation, technical scheme of the present invention is described further, but does not limit the present invention.
Embodiment 1:
A. prepare milk fermentation liquid: get whole-fat milk powder 100g, add the citric acid-sodium citrate buffer of 200gpH6.5, be uniformly mixed, be cooled to after room temperature through pasteurize, according to every gram of full-cream emulsion 10 5the inoculum concentration access Lactobacillus casei of cfu, according to every gram of full-cream emulsion 10 6the inoculum concentration access Rhizopus oryzae of individual spore, 45 DEG C of condition bottom fermentation 4h, obtain dairy fermentation liquid after pasteurize;
B. newborn enzymolysis liquid is prepared: get milk fermentation liquid 100g, anhydrous butter oil 5g, lecithin 0.1g, obtain homogeneous liquid through homogeneous after shearing dispersion, add lipase 0.01g after pasteurize, proteinase-10 .03g, carry out enzymolysis 0.5h in 55 DEG C, 95min goes out enzyme 15min, obtains newborn enzymolysis liquid;
C. thermal response cheese local flavor frankincense base is prepared: get newborn enzymolysis liquid 100g, add L-arginine 1.5g, L-PROLINE 1.5g, 1B 1.0g, L-Histidine 0.6g, L-Aspartic acid 0.5g, 5 '-flavour nucleotide disodium 0.6g, glucose 3.0g, fructose 1.0g, rhamnose 2.0g, glycerine 500g, adjustment pH to 6.5, be warming up to 95 DEG C of condensing reflux reaction 6h under stirring condition, be cooled to room temperature, after crossing colloid mill, obtain cheese's local flavor frankincense base.
Embodiment 2:
A. prepare milk fermentation liquid: get whole-fat milk powder 100g, add the citric acid-sodium citrate buffer of 400gpH6.0, be uniformly mixed, be cooled to after room temperature through pasteurize, according to every gram of full-cream emulsion 10 7the inoculum concentration access Lactobacillus casei of cfu, according to every gram of full-cream emulsion 10 4the inoculum concentration access Rhizopus oryzae of individual spore, 35 DEG C of condition bottom fermentation 10h, obtain dairy fermentation liquid after pasteurize;
B. newborn enzymolysis liquid is prepared: get milk fermentation liquid 100g, butter 10g, lecithin 1.0g, obtain homogeneous liquid through homogeneous after shearing dispersion, add lipase 0.03g after pasteurize, proteinase-10 .05g, carry out enzymolysis 2h in 50 DEG C, 95min goes out enzyme 15min, obtains newborn enzymolysis liquid;
C. thermal response cheese local flavor frankincense base is prepared: get newborn enzymolysis liquid 100g, add L-arginine 2.5g, L-PROLINE 3.5g, 1B 2.0g, L-Histidine 1.0g, L-Aspartic acid 1.5g, 5 '-flavour nucleotide disodium 1.5g, maltose 4.0, wood sugar 4.0, arabinose 1.0g, glucose 2.0g, propane diols 800g, adjustment pH to 6.5, be warming up to 110 DEG C of condensing reflux reaction 3h under stirring condition, be cooled to room temperature, after crossing colloid mill, obtain cheese's local flavor frankincense base.
Embodiment 3:
A. prepare milk fermentation liquid: get whole-fat milk powder 100g, add the citric acid-sodium citrate buffer of 800gpH5.0, be uniformly mixed, be cooled to after room temperature through pasteurize, according to every gram of full-cream emulsion 10 6the inoculum concentration access Lactobacillus casei of cfu, according to every gram of full-cream emulsion 10 5the inoculum concentration access Rhizopus oryzae of individual spore, 30 DEG C of condition bottom fermentation 24h, obtain dairy fermentation liquid after pasteurize;
B. newborn enzymolysis liquid is prepared: get milk fermentation liquid 100g, whipping cream 25g, lecithin 1.5g, obtain homogeneous liquid through homogeneous after shearing dispersion, add lipase 0.04g after pasteurize, proteinase-10 .10g, carry out enzymolysis 8h in 30 DEG C, 95min goes out enzyme 15min, obtains newborn enzymolysis liquid;
C. thermal response cheese local flavor frankincense base is prepared: get newborn enzymolysis liquid 100g, add L-arginine 7.5g, L-PROLINE 7.5g, 1B 5.0g, L-Histidine 2.5g, L-Aspartic acid 1.5g, 5 '-flavour nucleotide disodium 3.0g, fructose 6.0g, wood sugar 2.0, lactose 5.0g, glycerine 1000g, adjustment pH to 7.0, be warming up to 120 DEG C of condensing reflux reaction 1h under stirring condition, be cooled to room temperature, after crossing colloid mill, obtain cheese's local flavor frankincense base.

Claims (4)

1. a preparation method for cheese's local flavor frankincense base, is characterized in that comprising the following steps:
A. milk fermentation liquid is prepared: get whole-fat milk powder 100 parts, add the citric acid-sodium citrate buffer of 200 ~ 800 parts of pH5.0 ~ 6.5, be uniformly mixed, be cooled to after room temperature through pasteurize, add Lactobacillus casei and Rhizopus oryzae, 30 ~ 45 DEG C of condition bottom fermentation 4 ~ 24h, obtain dairy fermentation liquid after pasteurize; Described Lactobacillus casei addition is every gram of full-cream emulsion 10 5-10 7cfu; The addition of described Rhizopus oryzae is every gram of full-cream emulsion 10 4-10 6individual spore;
B. newborn enzymolysis liquid is prepared: get milk fermentation liquid 100 parts, butter oil 5 ~ 25 parts, 0.1 ~ 1.5 part, lecithin, homogeneous liquid is obtained through homogeneous after shearing dispersion, add 0.01 ~ 0.05 part, lipase after pasteurize, proteinase-10 .03 ~ 0.15 part, carry out enzymolysis 0.5 ~ 8h in 30 ~ 55 DEG C, 95min goes out enzyme 15min, obtains newborn enzymolysis liquid;
C. thermal response prepares cheese's local flavor frankincense base: get newborn enzymolysis liquid 100 parts, adds L-arginine 1.5 ~ 7.5 parts, L-PROLINE 1.5 ~ 7.5 parts, 1B 1.0 ~ 5.0 parts, L-Histidine 0.6 ~ 3.0 part, L-Aspartic acid 0.5 ~ 2.5 part, 5 '-flavour nucleotide disodium 0.6 ~ 3.0 part, reduced sugar 6.0 ~ 30.0 parts, solvent 200 ~ 1000 parts, adjustment pH to 6.5 ~ 7.5, are warming up to 95 ~ 120 DEG C of condensing reflux reaction 1 ~ 6h under stirring condition, be cooled to room temperature, after crossing colloid mill, obtain cheese's local flavor frankincense base.
2. cheese's local flavor frankincense base according to claim 1 and preparation method thereof, is characterized in that: described butter oil is anhydrous butter oil, butter or whipping cream.
3. cheese's local flavor frankincense base according to claim 1 and preparation method thereof, is characterized in that: described reduced sugar is glucose, fructose, wood sugar, arabinose, lactose, maltose, rhamnose or trehalose.
4. cheese's local flavor frankincense base according to claim 1 and preparation method thereof, is characterized in that: described solvent is water, propane diols or glycerine.
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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018202686A1 (en) * 2017-05-03 2018-11-08 Nestec S.A. Natural flavor base and process for its preparation
WO2018202683A1 (en) * 2017-05-03 2018-11-08 Nestec S.A. Natural flavor base and process for its preparation
CN108913357A (en) * 2018-08-30 2018-11-30 王婧婧 A kind of preparation method of red-jujube flavor essence
CN109077344A (en) * 2018-09-01 2018-12-25 王婧婧 A kind of preparation method of milk-taste essence
CN111150043A (en) * 2020-01-16 2020-05-15 恒枫食品科技有限公司 Milk flavor essence and preparation method thereof
CN111758875A (en) * 2020-07-30 2020-10-13 东莞波顿香料有限公司 Opacifier and preparation method and application thereof
CN112021546A (en) * 2020-09-04 2020-12-04 恒枫食品科技有限公司 Preparation method of almond-flavored essence
CN112535216A (en) * 2020-12-22 2021-03-23 合肥七哥食品有限责任公司 Preparation method of cheese-flavor baking and conditioning cream
CN113598342A (en) * 2021-07-27 2021-11-05 洽洽食品股份有限公司 Cheese-flavored melon seeds and processing method thereof
CN114982931A (en) * 2022-06-16 2022-09-02 仲恺农业工程学院 Cheese-flavored essence base and preparation method and application thereof
CN115191590A (en) * 2022-07-18 2022-10-18 广东馨杰生化科技有限公司 Cheese essence and preparation method thereof
CN112640961B (en) * 2020-12-23 2024-04-19 奶酪博士(上海)科技有限公司 Cheese carbonated beverage and preparation method thereof
JP7562422B2 (en) 2018-10-17 2024-10-07 フイルメニツヒ ソシエテ アノニム Emulsions for modifying the sensory properties of foods and beverages

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CN104187551A (en) * 2014-09-10 2014-12-10 王婧婧 Cake essence and preparation method thereof
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Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018202683A1 (en) * 2017-05-03 2018-11-08 Nestec S.A. Natural flavor base and process for its preparation
WO2018202686A1 (en) * 2017-05-03 2018-11-08 Nestec S.A. Natural flavor base and process for its preparation
CN108913357A (en) * 2018-08-30 2018-11-30 王婧婧 A kind of preparation method of red-jujube flavor essence
CN109077344A (en) * 2018-09-01 2018-12-25 王婧婧 A kind of preparation method of milk-taste essence
JP7562422B2 (en) 2018-10-17 2024-10-07 フイルメニツヒ ソシエテ アノニム Emulsions for modifying the sensory properties of foods and beverages
CN111150043B (en) * 2020-01-16 2023-08-25 恒枫食品科技有限公司 Milk flavor essence and preparation method thereof
CN111150043A (en) * 2020-01-16 2020-05-15 恒枫食品科技有限公司 Milk flavor essence and preparation method thereof
CN111758875A (en) * 2020-07-30 2020-10-13 东莞波顿香料有限公司 Opacifier and preparation method and application thereof
CN112021546A (en) * 2020-09-04 2020-12-04 恒枫食品科技有限公司 Preparation method of almond-flavored essence
CN112021546B (en) * 2020-09-04 2022-07-01 恒枫食品科技有限公司 Preparation method of almond-flavored essence
CN112535216A (en) * 2020-12-22 2021-03-23 合肥七哥食品有限责任公司 Preparation method of cheese-flavor baking and conditioning cream
CN112640961B (en) * 2020-12-23 2024-04-19 奶酪博士(上海)科技有限公司 Cheese carbonated beverage and preparation method thereof
CN113598342A (en) * 2021-07-27 2021-11-05 洽洽食品股份有限公司 Cheese-flavored melon seeds and processing method thereof
CN113598342B (en) * 2021-07-27 2023-06-30 洽洽食品股份有限公司 Cheese-flavored melon seeds and processing method thereof
CN114982931B (en) * 2022-06-16 2023-11-10 仲恺农业工程学院 Cheese flavor base and preparation method and application thereof
CN114982931A (en) * 2022-06-16 2022-09-02 仲恺农业工程学院 Cheese-flavored essence base and preparation method and application thereof
CN115191590A (en) * 2022-07-18 2022-10-18 广东馨杰生化科技有限公司 Cheese essence and preparation method thereof

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