CN105076249B - A kind of preparation method and application for preventing duckweed outburst growth inhibitor - Google Patents
A kind of preparation method and application for preventing duckweed outburst growth inhibitor Download PDFInfo
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- CN105076249B CN105076249B CN201510461743.3A CN201510461743A CN105076249B CN 105076249 B CN105076249 B CN 105076249B CN 201510461743 A CN201510461743 A CN 201510461743A CN 105076249 B CN105076249 B CN 105076249B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
A kind of preparation method for preventing duckweed outburst growth inhibitor.It is characterized in that the Chinese herbal medicine coptis, Official Rhubarb and Chinese herbal medicine cordate houttuynia after buying back respectively crush respectively, are sieved, it is fitted into the bolting silk bag that customization is 200 mesh, it is placed in 500ml beaker, distilled water is added by the solid-to-liquid ratio of 1:20, the slightly boiled 2h of water-bath, extracting solution is finally concentrated to 250ml, Chinese herbal medicine rhizoma extracting liquid, Official Rhubarb extracting solution and the Chinese herbal medicine Herba Houttuyniae extract that concentration is 0.1g/ml are finally each got, is then mixed according to the volume ratio of 5:1:1 up to prevention duckweed outburst growth inhibitor.Prevention duckweed outburst growth inhibitor of the invention is sowed by the volume ratio of itself and the water body 1:50 for needing restoration of the ecosystem into water body, that is, duckweed outburst growth can be effectively suppressed.Prevention duckweed outburst growth inhibitor of the invention may be applicable to inhibit duckweed outburst growth in the water body of restoration of the ecosystem.
Description
Technical field
The present invention relates to a kind of inhibitor, more particularly to a kind of preparation method for preventing duckweed outburst growth inhibitor,
Suitable for inhibiting duckweed outburst growth water body.
Background technique
In recent years, the unreasonable discharge of sanitary sewage, and it is floating that the intensive mode of agriculture of chemical fertilizer, which is widely used,
Duckweed growth provides the nutriments such as nitrogen abundant, phosphorus, the livestock and poultry cultivation mode of modern intensive make again its lose duck,
The natural enemies such as grass carp cause to be seen everywhere the duckweed for being covered on the water surface in the hydrostatic such as lakes and marhshes, ditch, reservoir, river or unhurried current.China
North and south various regions duckweed breaks out the time between the 4-7 month.Duckweed itself to nitrogen, phosphorus recycling have it is very strong function is absorbed and utilized, fit
Amount growth can purify water, and rich in nutrient protein (as livestock and poultry cultivation feed) and have medical value, but it breaks out formula life
Long but to bring serious environmental problem, harm specifically includes that pollution water quality, threatens drinking water safety；Navigation channel is covered, is hindered
Navigation, influences shipping safety；Polluted-water influences culture fishery sound development；Landscape is influenced, water ecology balance is destroyed
Deng, China every year because duckweed is administered and its caused by economic loss be up to 10,000,000,000 yuan or more.Therefore it is quick-fried to study effectively improvement duckweed
The technical method of hair is just increasingly paid attention to.
The control of duckweed outburst at present is based on physics salvaging and chemical herbicide.Method by manually or mechanically salvaging
Removal to duckweed is a kind of quick, direct, free of contamination mode, but is a kind of later period buffer approach broken out to duckweed,
The characteristic that duckweed is easily spread thoroughly remove it can not, and residual duckweed can sought because there is broader living space
It supports tachyauxesis when sufficient, light temperature physical and chemical factor is suitable for breed, can break out again again quickly.Chemical herbicide has residual poison
Property, lack drug effect specificity, and long-term unification uses tool resistance risk, it would be highly desirable to which research and development effectively inhibit the Chinese herbal medicine weeding of duckweed
Agent.
Summary of the invention
The invention aims to provide a kind of preparation method and application for preventing duckweed outburst growth inhibitor, to solve
The above-mentioned problems in the prior art, using prevention duckweed outburst growth inhibitor of the invention to the water for needing restoration of the ecosystem
Body can effectively inhibit the growth of duckweed, and inhibitory effect is good, inhibit high-efficient, and do not generate secondary pollution to water body.
To achieve the above object the present invention is implemented as follows: of the invention a kind of prevent duckweed outburst growth suppression
The preparation method of preparation includes the following steps:
1) Chinese herbal medicine rhizoma extracting liquid is prepared:
The Chinese herbal medicine coptis after buying back crushes, is sieved, and is fitted into the bolting silk bag that customization is 200 mesh, is placed in 500ml burning
In cup, distilled water, the slightly boiled 2h of water-bath are added by the solid-to-liquid ratio of 1:20, extracting solution is finally concentrated to 250ml to get concentration and is
The Chinese herbal medicine rhizoma extracting liquid of 0.1g/ml;
2) Official Rhubarb extracting solution is prepared:
Official Rhubarb after buying back crushes, is sieved, and is fitted into the bolting silk bag that customization is 200 mesh, is placed in 500ml burning
In cup, distilled water, the slightly boiled 2h of water-bath are added by the solid-to-liquid ratio of 1:20, extracting solution is finally concentrated to 250ml to get concentration and is
The Official Rhubarb extracting solution of 0.1g/ml;
3) Chinese herbal medicine Herba Houttuyniae extract is prepared:
Chinese herbal medicine cordate houttuynia after buying back crushes, is sieved, and is fitted into the bolting silk bag that customization is 200 mesh, is placed in 500ml
In beaker, distilled water, the slightly boiled 2h of water-bath are added by the solid-to-liquid ratio of 1:20, extracting solution is finally concentrated to 250ml to get concentration and is
The Chinese herbal medicine Herba Houttuyniae extract of 0.1g/ml;
4) by above-mentioned steps 1), step 2 and step 3) the Chinese herbal medicine rhizoma extracting liquid, the Official Rhubarb extracting solution that extract
With Chinese herbal medicine Herba Houttuyniae extract, the inhibitor up to prevention duckweed outburst growth is mixed according to the volume ratio of 5:1:1.
A kind of application method for preventing duckweed outburst growth inhibitor of the invention: being in the water body for needing restoration of the ecosystem
In, prevention duckweed outburst growth inhibitor is sowed by the volume ratio of itself and the water body 1:50 for needing restoration of the ecosystem into water body
?.
A kind of drug advantage for preventing duckweed outburst growth inhibitor of the invention is as follows:
1, main in the ingredient of the Chinese herbal medicine coptis to contain berberine, also contain coptisine, worenine, palmatine, medicine
A series of alkali components such as root alkali, table coptisine.In Chinese herbal medicine inhibitor, rhizoma extracting liquid plays main inhibition duckweed biology and increases
The effect of amount.
2, the main component of Official Rhubarb is anthraquinone glycoside and dianthracene ketone glucoside, and a large amount of presence of this ingredient promote rheum officinale to mention
Taking liquid to play auxiliary in matched Chinese herbal medicine inhibitor and enhancing the coptis inhibits the growth of duckweed blade quantity and biology to increase
Amount, is embodied in total fresh weight.
3, Chinese herbal medicine cordate houttuynia is cold in nature, and main antibiotic effective ingredient is volatile oil and decanoyl acetaldehyde, this constituents is to floating
Duckweed tissue and photosynthetical system have stimulation and damaging action to a certain extent.
In brief, China has Chinese herbal medicine resource abundant, the preferably novel Chinese herbal medicine inhibitor tool from the treasure-house
Have the advantages that environmental friendliness height, raw material are easy to get, are cheap.
Prevention duckweed outburst growth inhibitor is assessed in clinical drug effect below:
Experiment 1,
Material used in experiment is duckweed, is China lake, river waters Common Species, duckweed starting is planted in culture vessel
Strain body number is 60 (30 plants).
(1) drug is that prevention duckweed of the invention breaks out growth inhibitor (laboratory autogamy) in experiment.
(2) application method of prevention duckweed outburst growth inhibitor of the invention:
The beaker that culture vessel used is volume 1000ml is tested, culture solution is the lake water 1000ml of filtering, is held in culture
60 duckweed plant bodies uniform in size by strictly selecting are launched in device, and prevention duckweed outburst growth inhibition of the invention is added
Agent, when experiment, are divided into two groups, and the prevention duckweed of the invention that 30mL is added in the culture vessel of A group experimental group breaks out growth inhibition
Prevention duckweed outburst growth inhibitor of the invention is replaced by the filtered lake water of 30mL in B group control group by agent, and other conditions are not
Become；Then A group and B group are co-cultured into 96h, in for 24 hours, 48h, 72h, 96h record the numbers of blade of two groups of duckweeds respectively
Situation of change, and in the fresh weight for weighing two groups of duckweeds after the test.Experimental result is as shown in Figure 1 and Figure 2.It can be seen by Fig. 1
Out, the experimental group duckweed number of blade added with prevention duckweed outburst growth inhibitor of the invention is during observation almost without change
Change, inhibitory effect is obvious；And the control group duckweed growth for being not added with prevention duckweed outburst growth inhibitor of the invention is rapid, leaf
The piece number increases to 96 after 96h by initial 60.As seen from Figure 2, growth is broken out added with prevention duckweed of the invention
Duckweed fresh weight has almost no change after the experimental group 96h of inhibitor, and inhibitory effect is obvious；And it is not added with prevention duckweed of the invention
The control group duckweed growth for breaking out growth inhibitor is rapid, fresh weight increased to by 0.23 initial ± 0.003g after 96h 0.33 ±
0.005g。
Experiment 2,
Material used in this experiment is duckweed spirodela, is China lake, river waters Common Species, duckweed in culture vessel
Starting plant body number is 48 (24 plants).
(1) Chinese herbal medicine is that prevention duckweed of the invention breaks out growth inhibitor (laboratory autogamy).
(2) application method of prevention duckweed outburst growth inhibitor of the invention:
The beaker that culture vessel used is volume 1000ml is tested, culture solution is the lake water 1000ml of filtering, is held in culture
48 duckweed spirodela plant bodies uniform in size by strictly selecting are launched in device, and prevention duckweed outburst growth of the invention is added
Inhibitor, when experiment, are divided into two groups, and the prevention duckweed of the invention that 15mL is added in the culture vessel of A group experimental group breaks out growth
Prevention duckweed outburst growth inhibitor of the invention is replaced by the filtered lake water of 15mL in B group control group by inhibitor, other
Part is constant；Then A group and B group are co-cultured into 96h, in for 24 hours, 48h, 72h, 96h record the leaves of two groups of duckweeds respectively
The piece number situation of change, and the fresh weight of two groups of duckweeds is weighed after the test in 96h.Experimental result is as shown in Figure 3, Figure 4.By Fig. 3
As can be seen that the experimental group duckweed number of blade added with prevention duckweed outburst growth inhibitor of the invention during observation almost
Do not change, inhibitory effect is obvious；And it is not added with the control group duckweed growth of prevention duckweed outburst growth inhibitor of the invention
Rapidly, the number of blade increases to 64 after 96h by initial 48.As seen from Figure 4, quick-fried added with prevention duckweed of the invention
Duckweed fresh weight has almost no change after sending out the experimental group 96h of growth inhibitor, and inhibitory effect is obvious；And it is not added with of the invention pre-
The control group duckweed growth of anti-duckweed outburst growth inhibitor is rapid, after fresh weight increases to 96h by 0.25 initial ± 0.008g
0.36±0.005g。
The beneficial effects of the present invention are:
1, prevention duckweed of the invention outburst growth inhibitor can effectively inhibit the growth of duckweed, and duckweed growth presses down after application
Rate processed reaches 95% or more, and inhibitory effect is good, inhibits high-efficient, and do not generate secondary pollution, green, safety, ring to water body
It protects.
2, the raw material of prevention duckweed outburst growth inhibitor of the invention are the Chinese herbal medicine coptis, rheum officinale and cordate houttuynia, original
Material is easy to get, is cheap, at low cost, not by conditions such as region, solar term, temperature when being conducive to practical popularization and use, and using
Limitation.
Prevention duckweed outburst growth inhibitor of the invention can be suitable for inhibiting duckweed outburst growth application in water body.
Detailed description of the invention
The present invention is provided by embodiment below and its attached drawing.
Fig. 1 is to test prevention duckweed in 1 to break out growth inhibitor to the inhibitory effect comparison diagram of the duckweed number of blade, wherein in advance
Anti- duckweed outburst growth inhibitor usage amount is 30mL/L.
Fig. 2 is to test prevention duckweed in 1 to break out growth inhibitor to the inhibitory effect comparison diagram of duckweed fresh weight, wherein preventing
Duckweed growth inhibitor usage amount is 30mL/L.
Fig. 3 is to test in 2 prevention duckweed outburst growth inhibitor to the inhibitory effect comparison diagram of the duckweed spirodela number of blade,
Middle prevention duckweed outburst growth inhibitor usage amount is 15mL/L.
Fig. 4 be test in 2 prevention duckweed outburst growth inhibitor to the inhibitory effect comparison diagram of duckweed spirodela fresh weight, wherein
Preventing duckweed outburst growth inhibitor usage amount is 15mL/L.
Specific embodiment
Prevention duckweed outburst growth inhibitor of the invention is described in further detail below with reference to attached drawing.
Referring to Fig. 1, Fig. 2, Fig. 3 and Fig. 4, the prevention duckweed outburst growth inhibitor preparation method of the invention includes following step
It is rapid:
1) Chinese herbal medicine rhizoma extracting liquid is prepared:
The Chinese herbal medicine coptis after buying back crushes, is sieved, and is fitted into the bolting silk bag that customization is 200 mesh, is placed in 500ml burning
In cup, distilled water, the slightly boiled 2h of water-bath are added by the solid-to-liquid ratio of 1:20, extracting solution is finally concentrated to 250ml to get concentration and is
The Chinese herbal medicine rhizoma extracting liquid of 0.1g/ml;
2) Official Rhubarb extracting solution is prepared:
Official Rhubarb after buying back crushes, is sieved, and is fitted into the bolting silk bag that customization is 200 mesh, is placed in 500ml burning
In cup, distilled water, the slightly boiled 2h of water-bath are added by the solid-to-liquid ratio of 1:20, extracting solution is finally concentrated to 250ml to get concentration and is
The Official Rhubarb extracting solution of 0.1g/ml;
3) Chinese herbal medicine Herba Houttuyniae extract is prepared:
Chinese herbal medicine cordate houttuynia after buying back crushes, is sieved, and is fitted into the bolting silk bag that customization is 200 mesh, is placed in 500ml
In beaker, distilled water, the slightly boiled 2h of water-bath are added by the solid-to-liquid ratio of 1:20, extracting solution is finally concentrated to 250ml to get concentration and is
The Chinese herbal medicine Herba Houttuyniae extract of 0.1g/ml;
4) by above-mentioned steps 1), step 2 and step 3) the Chinese herbal medicine rhizoma extracting liquid, the Official Rhubarb extracting solution that extract
With Chinese herbal medicine Herba Houttuyniae extract, the inhibitor up to prevention duckweed outburst growth is mixed according to the volume ratio of 5:1:1.
A kind of application method for preventing duckweed outburst growth inhibitor in the water body for needing restoration of the ecosystem of the invention is
In the water body for needing restoration of the ecosystem, by prevention duckweed outburst growth inhibitor by it with the water body 1:50's for needing restoration of the ecosystem
Volume ratio is sowed into water body.
Claims (2)
1. a kind of preparation method for preventing duckweed outburst growth inhibitor, it is characterized in that including the following steps:
1) Chinese herbal medicine rhizoma extracting liquid is prepared:
The Chinese herbal medicine coptis after buying back crushes, is sieved, and is fitted into the bolting silk bag for 200 mesh, is placed in 500ml beaker, by 1:
20 solid-to-liquid ratio adds distilled water, the slightly boiled 2h of water-bath, and extracting solution is finally concentrated to the medium-height grass that 250ml is 0.1g/ml to get concentration
Medicine rhizoma extracting liquid;
2) Official Rhubarb extracting solution is prepared:
Official Rhubarb after buying back crushes, is sieved, and is fitted into the bolting silk bag for 200 mesh, is placed in 500ml beaker, by 1:
20 solid-to-liquid ratio adds distilled water, the slightly boiled 2h of water-bath, and extracting solution is finally concentrated to the medium-height grass that 250ml is 0.1g/ml to get concentration
Medicine Radix Et Rhizoma Rhei extract;
3) Chinese herbal medicine Herba Houttuyniae extract is prepared:
Chinese herbal medicine cordate houttuynia after buying back crushes, is sieved, and is fitted into the bolting silk bag for 200 mesh, is placed in 500ml beaker, presses
The solid-to-liquid ratio of 1:20 adds distilled water, the slightly boiled 2h of water-bath, and it is in 0.1g/ml that extracting solution, which is finally concentrated to 250ml to get concentration,
Herbal medicine Herba Houttuyniae extract;
4) by above-mentioned steps 1), step 2 and step 3) extract Chinese herbal medicine rhizoma extracting liquid, Official Rhubarb extracting solution and in
Herbal medicine Herba Houttuyniae extract mixes the inhibitor up to prevention duckweed outburst growth according to the volume ratio of 5:1:1.
2. a kind of application for preventing duckweed outburst growth inhibitor in the water body for needing restoration of the ecosystem according to claim 1,
It is characterized in that in the water body for needing restoration of the ecosystem, by prevention duckweed outburst growth inhibitor by itself and the water for needing restoration of the ecosystem
The volume ratio of body 1:50 is sowed into water body.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390526A (en) * | 2008-11-06 | 2009-03-25 | 天津农学院 | Microcystic aeruginosa Chinese herbal medicine algae-inhibition agent and preparation method thereof |
| CN103461390A (en) * | 2013-09-11 | 2013-12-25 | 安徽师范大学 | Inhibition method of microcystis aeruginosa |
| CN103936125A (en) * | 2014-04-18 | 2014-07-23 | 芜湖凯奥尔环保科技有限公司 | Rare-earth blue algae treating agent and preparation method thereof |
| CN103936095A (en) * | 2014-04-18 | 2014-07-23 | 芜湖凯奥尔环保科技有限公司 | High-adsorbability blue algae treatment agent and manufacturing method thereof |
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| US5514779A (en) * | 1991-06-07 | 1996-05-07 | Zeneca Limited | Biocidal proteins from plants |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390526A (en) * | 2008-11-06 | 2009-03-25 | 天津农学院 | Microcystic aeruginosa Chinese herbal medicine algae-inhibition agent and preparation method thereof |
| CN103461390A (en) * | 2013-09-11 | 2013-12-25 | 安徽师范大学 | Inhibition method of microcystis aeruginosa |
| CN103936125A (en) * | 2014-04-18 | 2014-07-23 | 芜湖凯奥尔环保科技有限公司 | Rare-earth blue algae treating agent and preparation method thereof |
| CN103936095A (en) * | 2014-04-18 | 2014-07-23 | 芜湖凯奥尔环保科技有限公司 | High-adsorbability blue algae treatment agent and manufacturing method thereof |
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