CN105067545B - A kind of thimerosal concentration detection method and the concentration monitoring device using this method - Google Patents
A kind of thimerosal concentration detection method and the concentration monitoring device using this method Download PDFInfo
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Abstract
The invention discloses a kind of thimerosal concentration detection method and the concentration monitoring devices of use this method.This thimerosal concentration detection method and thimerosal stoste is quantitatively extracted to reaction tube from thimerosal storage box using the thimerosal quantitative sampling device that is equipped with of concentration monitoring device of this method, detect the absorbance of thimerosal stoste, water is extracted to reaction tube from water inlet, the thimerosal sample copy of prepared and diluted concentration, detect the absorbance of thimerosal sample, it records and draws corresponding standard curve, when sterilizing machine is run, the automatic thimerosal extracted in use is to reaction tube, absorbance is detected by detector, then sentence read result is compared by machine, whole process can be automatically performed by machine, monitoring in real time, greatly improve the accuracy of concentration monitor, it avoids sterilizing failure caused by due to thimerosal concentration is insufficient, time saving and energy saving and manufacturing cost is low.The present invention is suitable for disinfecting apparatus field.
Description
Technical field
The present invention relates to disinfecting apparatus fields, more particularly to a kind of thimerosal concentration detection method and use this method
Concentration monitoring device.
Background technology
Currently, medical endoscope will carry out cleaning and sterilizing after each could be used for next patient, cleaning disappears
Poison is generally sterilized using artificial disinfection way or automatic endoscope cleaning-disinfection machine.One of crucial step is soaked with thimerosal
Bubble a period of time, to kill the contaminating microorganisms being attached on scope, to avoid patient's cross-infection.Disinfection Effect and thimerosal
Concentration is related with action time, therefore to ensure qualified Disinfection Effect, it is necessary to using normal concentration thimerosal and act on
It is more than the defined time.Some thimerosals are disposable, and have a kind of thimerosal can multiple Reusability.Mesh
The preceding common sterilizing endoscope liquid of hospital has glutaraldehyde, o-phthalaldehyde etc., glutaraldehyde and o-phthalaldehyde to be when packing
Relatively stablize, can store 1 year or more and not fail, but after use of breaking seal, the term of validity generally only 15 days or so.Penta 2
Aldehyde and o-phthalaldehyde are the thimerosals that may be reused, the standardization program of endoscope cleaning disinfection be generally flushing → enzyme wash →
Cleaning → disinfection → rinsing → drying, therefore when thimerosal uses every time, to be all mixed into previous step in a small amount of scope and pipeline residual
The water stayed so that thimerosal is diluted, and concentration can constantly decline.Therefore the concentration of often monitoring thimerosal is needed in use,
Ensure in prescribed limit, just can guarantee qualified Disinfection Effect, when thimerosal concentration drops to certain value, general glutaraldehyde
Thimerosal failure concentration is about the 90% of original concentration, and the failure concentration of o-phthalaldehyde is about the 65% of original concentration, cannot
Ensure Disinfection Effect, it is therefore desirable to which concentration monitor is carried out to the thimerosal in using.
The monitoring means generally used at present is to use Test paper（Colour developing card）It is immersed in thimerosal after 2 seconds and takes out drip
It is dry, colorimetric is carried out with standard color version in colour developing 5 to 8 minutes, judges whether thimerosal concentration is qualified by naked eyes.Hospitals at Present is common
It is to use test paper in the method for thimerosal glutaraldehyde, o-phthalaldehyde concentration monitor（Colour developing card）Colour developing, visually with standard color version
The method of colorimetric.This method needs manual operation, carries out colorimetric, artificial interpretation at the appointed time, and test paper is certain
The color difference unobvious showed in concentration range, resultant error is larger, and time-consuming and laborious.The colour developing card of general glutaraldehyde is shown
Color be yellow, and o-phthalaldehyde colour developing card display color be purple, two kinds of color distinctions are very big, to
The color that two kinds of Different lightwaves of automatic interpretation length is applicable on one machine is then very high to the requirement of detector, and machine cost is expensive.
Although having the concentration that more document reports can simply use ultraviolet absorption method directly to test glutaraldehyde now,
It is found by actual test, ultraviolet absorption method is only effective to the senaside of storing state (stable state), and actually uses
Be usually alkaline glutaric dialdehyde, needed before use be added pH adjusting agent.Alkaline glutaric dialdehyde is unstable, it may occur that polymerisation, this
Be also the shorter reason of the glutaraldehyde term of validity after use, the rate of absorbing UV of alkaline glutaric dialdehyde be it is continually changing at any time,
Therefore ultraviolet absorption method is not particularly suited for carrying out concentration monitor to the glutaral disinfectant solution in using.
Invention content
To solve the above problems, the present invention provides a kind of thimerosal Concentration Testing detecting thimerosal concentration using method of comparison
The concentration monitoring device of thimerosal concentration in method and automatic detection sterilizing machine.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of thimerosal concentration detection method, includes the following steps：
a）, at least prepare two kinds of concentration thimerosal, detect the absorbance of each concentration thimerosal；
b）, each concentration and corresponding absorbance are drawn to the standard curve for being used for concentration calibration accordingly；
c）, detection thimerosal sample absorbance；
d）, the absorbance that measures thimerosal sample and standard curve compare, the relative concentration for the liquid sample that is sterilized.
It is further used as the improvement of technical solution of the present invention, step c）In, thimerosal sample uses after being mixed with test fluid
Photoelectric colorimetry measures absorbance.
A kind of concentration monitoring device being monitored using thimerosal concentration detection method, including place the disinfection of thimerosal
Liquid storage box and the reaction tube for being used to prepare thimerosal detection sample, are equipped on the export pipeline of reaction tube for detecting disinfection
The detector of liquid absorbance, reaction tube are equipped with water inlet, are equipped between reaction tube and thimerosal storage box for quantitatively extracting
Thimerosal to reaction tube thimerosal quantitative sampling device.
It is further used as the improvement of technical solution of the present invention, reaction tube is also associated with the test fluid bottle equipped with test fluid, instead
It should manage and be equipped between test fluid bottle for quantitatively extracting test fluid to the test fluid quantitative sampling device of reaction tube.
It is further used as the improvement of technical solution of the present invention, the inlet of thimerosal quantitative sampling device connects water inlet,
Thimerosal quantitative sampling device is equipped with outlet, and outlet is back to thimerosal storage box.
It is further used as the improvement of technical solution of the present invention, the outer wall of reaction tube is equipped with heating device for heating and control
The temperature sensor of temperature processed, the air pump I of reaction tube being equipped with for drying up reaction tube or mixing liquid.
It is further used as the improvement of technical solution of the present invention, thimerosal quantitative sampling device and test fluid quantitative sampling device
Using plunger pump.
It is further used as the improvement of technical solution of the present invention, thimerosal quantitative sampling device and test fluid quantitative sampling device
Using quantity tube.
It is further used as the improvement of technical solution of the present invention, test fluid quantitative sampling device includes quantity tube II and testing
The air pump III for entering quantity tube II for blow pressure test fluid that liquid bottle is equipped with, quantity tube II are equipped with liquid level sensor.
It is further used as the improvement of technical solution of the present invention, thimerosal quantitative sampling device is equipped with quantity tube I, quantity tube I
Equipped with the liquid pump for extracting thimerosal.
Beneficial effects of the present invention：It this thimerosal concentration detection method and is equipped with using the concentration monitoring device of this method
Thimerosal quantitative sampling device quantitatively extracts thimerosal stoste to reaction tube from thimerosal storage box, detects the suction of thimerosal stoste
Luminosity extracts water to reaction tube from water inlet, and the thimerosal sample copy of prepared and diluted concentration detects the extinction of thimerosal sample
Degree records and draws corresponding standard curve, and when sterilizing machine is run, the automatic thimerosal extracted in use passes through to reaction tube
Detector detects absorbance, then compares sentence read result by machine, and whole process can be automatically performed by machine, monitors in real time, greatly
The big accuracy for improving concentration monitor avoids sterilizing failure caused by due to thimerosal concentration is insufficient, time saving and energy saving and be manufactured into
This is low.
Description of the drawings
The invention will be further described below in conjunction with the accompanying drawings：
Fig. 1 is first embodiment of the invention overall structure diagram；
Fig. 2 is second embodiment of the invention overall structure diagram；
Fig. 3 is glutaral disinfectant solution A brands absorbance of the present invention and linear graph formed by concentration；
Fig. 4 is o-phthalaldehyde thimerosal B brands absorbance of the present invention and linear graph formed by concentration；
Fig. 5 is o-phthalaldehyde thimerosal C brands absorbance of the present invention and linear graph formed by concentration；
Fig. 6 is o-phthalaldehyde thimerosal D brands absorbance of the present invention and linear graph formed by concentration.
Specific implementation mode
The technique effect of the design of the present invention, concrete structure and generation is carried out below with reference to embodiment and attached drawing clear
Chu is fully described by, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is this hair
Bright a part of the embodiment, rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art are not being paid
The other embodiment obtained under the premise of creative work, belongs to the scope of protection of the invention.
Referring to Fig.1~Fig. 6, the present invention are a kind of thimerosal concentration detection method, are included the following steps：
a）, at least prepare two kinds of concentration thimerosal, detect the absorbance of each concentration thimerosal；
b）, each concentration and corresponding absorbance are drawn to the standard curve for being used for concentration calibration accordingly；
c）, detection thimerosal sample absorbance；
d）, the absorbance that measures thimerosal sample and standard curve compare, the relative concentration for the liquid sample that is sterilized.
As the preferred embodiment of the present invention, step c）In, thimerosal sample uses photoelectricity ratio after being mixed with test fluid
Color method measures absorbance.
As the preferred embodiment of the present invention, step a）In, the thimerosal concentration of configuration includes containing volume fraction 100%
The thimerosal of sterilized stock solution and containing volume fraction be 50% sterilized stock solution thimerosal.
Standard curve can be drawn with the absorbance index of multiple and different concentration, concentration range is 1%~body in volume fraction
Fraction is in 100%.
A kind of concentration monitoring device being monitored using thimerosal concentration detection method, including place the disinfection of thimerosal
Liquid storage box 1 and the reaction tube 2 for being used to prepare thimerosal detection sample, are equipped on the export pipeline of reaction tube 2 for detecting
The detector 4 of thimerosal absorbance, reaction tube 2 are equipped with water inlet 21, are equipped with and use between reaction tube 2 and thimerosal storage box 1
In the thimerosal quantitative sampling device of quantitative extraction thimerosal to reaction tube 2.
It this thimerosal concentration detection method and is filled using the thimerosal quantitative sampling that is equipped with of concentration monitoring device of this method
It sets and quantitatively extracts thimerosal stoste from thimerosal storage box 1 to reaction tube 2, the absorbance of thimerosal stoste is detected, from water inlet
21 extract water to reaction tube 2, and the thimerosal sample copy of prepared and diluted concentration detects the absorbance of thimerosal sample, records and paint
Corresponding standard curve is made, when sterilizing machine is run, the automatic thimerosal extracted in use is detected to reaction tube 2 by detector 4
Then absorbance compares sentence read result by machine, whole process can be automatically performed by machine, monitors in real time, greatly improves concentration
The accuracy of monitoring avoids sterilizing failure caused by due to thimerosal concentration is insufficient, and time saving and energy saving and manufacturing cost is low.
As the preferred embodiment of the present invention, reaction tube 2 is also associated with the test fluid bottle 3 equipped with test fluid, reaction tube 2
It is equipped between test fluid bottle 3 for quantitatively extracting test fluid to the test fluid quantitative sampling device of reaction tube 2.
As the preferred embodiment of the present invention, the inlet of thimerosal quantitative sampling device connects water inlet 21, disinfection
Liquid quantitative sampling device is equipped with outlet 8, and outlet 8 is back to thimerosal storage box 1.
As the preferred embodiment of the present invention, the outer wall of reaction tube 2 is equipped with heating device 22 for heating and control
The temperature sensor 24 of temperature, the air pump I 7 of reaction tube 2 being equipped with for drying up reaction tube 2 or mixing liquid.
Heating device 22 is heating pad pasting.
As the preferred embodiment of the present invention, thimerosal quantitative sampling device and test fluid quantitative sampling device use column
Plug pump 5.
5 front end of plunger pump of thimerosal quantitative sampling device is equipped with check valve.
As the preferred embodiment of the present invention, thimerosal quantitative sampling device and test fluid quantitative sampling device are using fixed
Buret.
As the preferred embodiment of the present invention, test fluid quantitative sampling device includes quantity tube II 62 and in test fluid bottle
3 air pumps III 31 for entering quantity tube II 62 for blow pressure test fluid being equipped with, quantity tube II 62 are equipped with liquid level sensor 64.
Quantity tube II and test fluid bottle 3 are equipped with air filter.
As the preferred embodiment of the present invention, thimerosal quantitative sampling device is equipped with quantity tube I 61, and quantity tube I 61 is set
It is useful for extracting the liquid pump 63 of thimerosal.
As the preferred embodiment of the present invention, reaction tube 2 is equipped with blow vent 23.
As the preferred embodiment of the present invention, detector 4 is photo-diode pipe detector.Detector 4 is additionally provided with conductance
Sensor.
The present invention and automatic endoscope cleaning-disinfection machine are mating, for monitoring the dense of glutaraldehyde in machine or o-phthalaldehyde
Degree.The present invention provides a kind of test fluid, colour developing is kept the temperature after being mixed with quantitative thimerosal, so using the method for photoelectric colorimetry by machine
Device sentence read result greatly improves the accuracy of concentration monitor, avoids sterilizing failure caused by due to thimerosal concentration is insufficient.Entirely
Process can be automatically performed by machine, time saving and energy saving.
The light absorption detector of test fluid Same Wavelength can be suitable for glutaraldehyde simultaneously and two kinds of o-phthalaldehyde disappears
The manufacturing cost of venom, detector is much lower.The glutaraldehyde of common several brands and adjacent benzene two by test fluid and in the market
Formaldehyde thimerosal sample is tested, and as a result show can carry out chromogenic reaction to them.It is generated after being reacted with glutaraldehyde transparent
The product of colour generation can generate light absorption to the light in a wavelength range；It is shallower with o-phthalaldehyde reaction product color, but its
Reaction product can assemble to form colloidal solution, have absorption to the light of different wave length.Therefore using the light source of Same Wavelength
It is carried out at the same time detection, the filtering apparatus such as grating need not be used, only needs common laser diode as light source.Pass through survey
Test solution is to the absorbance of the thimerosal example reaction product of several different brands as a result, the suction that display different brands thimerosal generates
Shading value is different, but its absorbance is all linear with its concentration in using concentration range.Therefore, if
When thimerosal begins to use concentration, machine is calibrated automatically, that is, sampling the stoste of two parts of volumes, to be detected its a concentration of
100%, write down its absorbance；It takes the sample that a stoste adds a water dilution i.e. a concentration of 50% to be detected again, writes down its suction
Luminosity.The two data points are drawn a straight line to get to the standard curve of concentration calibration.Thimerosal sample after using
The absorbance measured is compared with standard curve, you can its relative concentration declined is learnt, to judge whether it fails.
The specific embodiment of the invention one：
Monitoring device mainly by thimerosal quantitative sampling device and test fluid quantitative sampling device, reaction tube 2, detector 4,
Several solenoid valves, a few part compositions.The quantitative addition of two of which liquid is all completed by plunger pump 5, and plunger pump 5 is one
Kind of flow can accurate quantification control precision pump.Thimerosal and test fluid are quantitatively input in reaction tube 2 respectively to be mixed, heating
It is reacted five minutes under heat-retaining condition, then passes through the detection of detector 4, thimerosal concentration is determined from colour developing depth.
Under standby mode, all solenoid valves are normally off.When starting when survey, triple valve 1 is first in upper left and leads to shape
State, triple valve 7 17 are adjusted to left and right and lead to state, and starting plunger pump 5 makes thimerosal be stored to thimerosal in pipeline inner circulating reflux
In case 1, it is therefore an objective to first rinse out thimerosal when residual in pipeline is last to test.Then the solenoid valve of blow vent 23 2 12 dozens
It opens, triple valve 1 switchs to lower-left and leads to state, and the plunger pump 5 that thimerosal quantitative sampling device is controlled by motherboard circuit will be certain
Amount, the thimerosal of two parts of volumes is injected into reaction tube 2 altogether, while the plunger pump 5 for controlling test fluid quantitative sampling device will
A certain amount of test fluid injects reaction tube 2, is all closed with latter two plunger pump 5.Open solenoid valve 5 15, while intermittent start
Air pump I 7 makes a small amount of air be blown into from the bottom of reaction tube 2, plays the role of being thoroughly mixed.Be then shut off solenoid valve 2 12,
Solenoid valve 5 15 starts heating device 22, so that 2 temperature of reaction tube is maintained by 24 feedback control heating power of temperature sensor and set
Constant temperature degree stops heating after five minutes.Then solenoid valve 2 12, solenoid valve 6 16 are opened, reaction solution flows through detector 4, works as electricity
When derivative sensor detects that liquid passes through, data that 4 start recording of detector measures and include being shown in machine by thimerosal concentration
In display screen.It is then turned off solenoid valve 2 12, inlet valve 13 to bottom right is opened and leads to state, reaction tube 2 and downstream tube are rinsed in water inlet
Inlet valve 13 is closed in road after 10 seconds.Booster air pump I 7 opens solenoid valve 4 14, and reaction tube 2 and downstream line are dried up with air-flow,
It gets ready to detect next time, turns off all components, complete detection.
The type and dosage of additive contained by thimerosal due to different brands are different, may be shown to test fluid
Color result has some impact on, and the present invention creatively eliminates interference using the method for detection relative concentration.In endoscope cleaning
After sterilizing machine has executed the step of replacing thimerosal every time, i.e., new thimerosal is calibrated automatically when beginning to use, and is first carried out primary
Above-mentioned standard detects, and takes two parts of volume sterilization liquid detection absorbances, and detectable concentration result is recorded in machine memory, marks
It is 100%；50% Concentration Testing is executed again, i.e., runs to when thimerosal is injected reaction tube 2 by above-mentioned sequence of movement, only note
Enter a volume sterilization liquid, then triple valve 1 go back to upper left lead to state, triple valve 7 17 go to upper right lead to state, open into
Water valve 13 to lower-left leads to state, and starting the operation of plunger pump 5 makes to be full of water in pipeline for a period of time, and extra water flows into discharge outlet,
It is then shut off triple valve 7 17, triple valve 1, which is switched to lower-left, leads to state, and mainboard control plunger pump 5 injects the water of a volume
Into reaction tube 2, to obtain the sample of 50% concentration.It is then shut off triple valve 1, inlet valve 13, continues to execute follow-up note
Enter test fluid and insulation reaction, detection, the concentration results detected are also recorded in machine memory, be labeled as 50%.
100% and 50% two data points are drawn a straight line for calibrating to get standard curve.In this thimerosal service life
In each monitoring when, take two parts of volume samples, acquired results to be compared, obtain with standard curve by standardization program detection
One relative concentration values sends out prompting message when relative concentration is less than the setting value of corresponding thimerosal.
The preferred embodiment of the present invention two：
At high price since plunger pump 5 belongs to precision element, the present invention provides a kind of alternative solution, use is special
Quantity tube replace plunger pump 5 realize quantitative liquid-filling function.Its structural principle is as shown in Fig. 2, and wherein liquid pump 63 only needs to use
Miniature diaphragm pump can cancel check valve since diaphragm pump has the function of check valve.
When test starts, triple valve 1 is in upper left and leads to state, starts liquid pump 63, and it is logical to left and right to open triple valve 7 17
State, thimerosal circulation flushing are simultaneously filled with quantity tube I 61, are then shut off triple valve 1 and liquid pump 63, booster air pump II 81,
Solenoid valve 8 18 is opened, with the extra thimerosal of air-flow blowout upper pipe, triple valve 7 17 is then turned off, opens breather valve
12, triple valve 1 is gone into logical state up and down, by the thimerosal injection reaction tube 2 in quantity tube I 61, is then turned off air pump
II 81, solenoid valve 8 18, triple valve 1 complete a volume sterilization liquid sampling；Primary all of above action is repeated, it is complete
At the injection of two parts of volume sterilization liquid samples.Test fluid is pressed into quantity tube II 62 by booster air pump III 31, when quantity tube II 62
When middle liquid level rises to liquid level sensor 64, air pump III 31 is closed, opens breather valve 10, test liquid level leans in quantity tube II 62
Gravity reflux is completed quantitative to injection open height.Breather valve 10 is closed after five seconds, opens solenoid valve 9 19, is made quantitative
Test fluid in pipe II 62 flows into reaction tube 2, is then shut off solenoid valve 9 19, completes the quantitative injection of test fluid.It is subsequent
The everythings such as heating reaction and detection are identical with the first embodiment.Calibration actions when newly changing thimerosal are also real with first
It is similar to apply example.It first executes to cancel venom twice and act to obtain two parts of volume sterilization liquid by standardization program and is tested to obtain concentration
For 100% data point；It reruns the calibration test at a 50% concentration numbers strong point, i.e. first operation is completed primary to cancel the dynamic of venom
Make, after injecting a volume sterilization liquid, triple valve 1 goes back to upper left and leads to state, and triple valve 7 17 goes to upper right and leads to state, into
Water valve 13, which goes to the logical state in lower-left, makes quantity tube I 61 be full of water, is then shut off inlet valve 13, and booster air pump II 81 opens electromagnetism
Valve 8 18 is then turned off triple valve 7 17, solenoid valve 2 12 is opened, by triple valve with the extra water of air-flow blowout upper pipe
One 11 go to logical state up and down, by the water injection reaction tube 2 of a volume in quantity tube I 61, are then turned off air pump II 81, electricity
Magnet valve 8 18, triple valve 1, to complete the sampling of 50% concentration thimerosal and continue subsequent detection calibration.
Certainly, the invention is not limited to the above embodiment, and those skilled in the art are without prejudice to originally
Equivalent variations or replacement can be also made under the premise of spirit, these equivalent deformations or replacement are all contained in the application right
It is required that in limited range.
Claims (2)
1. a kind of thimerosal concentration detection method, which is characterized in that include the following steps：
A) thimerosal that two kinds of concentration, is prepared by concentration monitoring device, detects the absorbance of each concentration thimerosal, configuration disappears
Venom concentration includes the thimerosal of 100% sterilized stock solution containing volume fraction and the disinfection containing volume fraction for 50% sterilized stock solution
Liquid；
The concentration monitoring device includes the thimerosal storage box (1) for placing thimerosal, is used to prepare thimerosal detection sample
Reaction tube (2) and several solenoid valves are equipped on the export pipeline of the reaction tube (2) for detecting thimerosal absorbance
Detector (4), the reaction tube (2) are equipped with water inlet (21), and reaction tube (2) upper end is equipped with blow vent (23), described
It is equipped between reaction tube (2) and thimerosal storage box (1) for quantitatively extracting thimerosal to the thimerosal quantitative of reaction tube (2)
Sampling device, the reaction tube (2) are also associated with the test fluid bottle (3) equipped with test fluid, the reaction tube (2) and test fluid bottle
(3) it is equipped between for quantitatively extracting test fluid to the test fluid quantitative sampling device of reaction tube (2), the thimerosal quantitative
The inlet connection water inlet (21) of sampling device, the thimerosal quantitative sampling device are equipped with outlet (8), the outlet
(8) it is back to thimerosal storage box (1), the thimerosal quantitative sampling device and test fluid quantitative sampling device use plunger pump
(5), the solenoid valve is normally off and includes triple valve one (11), triple valve seven (17), solenoid valve two (12), solenoid valve
Five (15), solenoid valve six (16), inlet valve (13), solenoid valve four (14)；
Triple valve one (11) is first in upper left and leads to state, and triple valve seven (17) is adjusted to left and right and leads to state, and starting plunger pump (5) makes to disappear
Venom is in pipeline inner circulating reflux to thimerosal storage box 1, and the solenoid valve two (12) on blow vent (23) is opened, triple valve one
(11) switch to lower-left and lead to state, the plunger pump (5) of thimerosal quantitative sampling device is controlled by a certain amount of two parts by motherboard circuit
The thimerosal of volume is injected into reaction tube (2), while the plunger pump (5) for controlling test fluid quantitative sampling device will be a certain amount of
Test fluid injects reaction tube (2), is all closed with latter two plunger pump (5)；Solenoid valve two (12), solenoid valve six (16) are opened, instead
Liquid stream device (4) after testing are answered, detect absorbance, and detectable concentration result is recorded in machine memory, are labeled as 100%；
When thimerosal is injected reaction tube (2), a volume sterilization liquid is only injected, then triple valve one (11) goes back to the logical shape in upper left
State, triple valve seven (17) go to upper right and lead to state, open inlet valve (13) to lower-left and lead to state, start plunger pump (5) and run one
The section time makes to be full of water in pipeline, and extra water flows into discharge outlet, is then shut off triple valve seven (17), and triple valve one (11) is turned
Lead to state for lower-left, mainboard controls plunger pump (5) and injects in the water to reaction tube (2) of a volume, to obtain 50% concentration
Sample；Triple valve one (11), inlet valve (13) are closed, continues to execute and is subsequently implanted into test fluid and detecting step, detection obtains
Concentration results be also recorded in machine memory, be labeled as 50%；
B), by the thimerosal of 100% sterilized stock solution containing volume fraction and containing volume fraction be 50% sterilized stock solution thimerosal and
The corresponding obtained data point of absorbance draws a straight line the two respectively, is as used for the standard curve of concentration calibration；
C) absorbance for, detecting thimerosal sample, suction is measured after thimerosal sample is mixed with test fluid using photoelectric colorimetry
Luminosity；
D) absorbance and the standard curve comparison for, measuring thimerosal sample, the relative concentration for the liquid sample that is sterilized.
2. thimerosal concentration detection method according to claim 1, it is characterised in that：The outer wall of the reaction tube (2) is set
It is useful for the heating device (22) of heating and controls the temperature sensor (24) of temperature, being equipped with for the reaction tube (2) is used for
Dry up reaction tube (2) or the air pump I (7) of mixing liquid.
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| 戊二醛浓度监测盒及戊二醛浓度监测方法;胡孝国等;《医疗卫生装备》;20071231;第 28 卷(第 2 期);第26页右栏第1段-第29页最后1段 * |
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