CN101458216A - Indicator composition for detection concentration of glutaraldehyde disinfectant - Google Patents
Indicator composition for detection concentration of glutaraldehyde disinfectant Download PDFInfo
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- CN101458216A CN101458216A CNA200710195734XA CN200710195734A CN101458216A CN 101458216 A CN101458216 A CN 101458216A CN A200710195734X A CNA200710195734X A CN A200710195734XA CN 200710195734 A CN200710195734 A CN 200710195734A CN 101458216 A CN101458216 A CN 101458216A
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Abstract
The invention relates to a chemical indicator composition for determining concentration of glutaraldehyde in glutaraldehyde disinfection solution. The chemical indicator composition consists of sulfite, amino acid and a buffer solution regulator and is contained in a glass tube or a transparent plastic tube with a plug. The composition is prepared according to the following principles: an addition reaction occurs between the sulfite in the indicator and the glutaraldehyde with the corresponding mol ratio in the glutaraldehyde disinfection solution, and if the remaining glutaraldehyde in the disinfection solution does not take part in the addition reaction, the remaining glutaraldehyde reacts with the amino acid in the indicator and develops yellow; if the glutaraldehyde in the disinfection solution reacts with the sulfite completely, the disinfection solution develops no color. Therefore, a specified amount of the indicator can be employed to indicate whether the content of the glutaraldehyde in the glutaraldehyde disinfection solution satisfies the minimum operation requirement for concentration. The tested glutaraldehyde disinfection solution does not develop any color, which means that the content of the glutaraldehyde in the disinfection solution is less than 1.8%; and the disinfection solution develops pale yellow or yellow, which means that the content of the glutaraldehyde in the glutaraldehyde disinfection solution is not less than 1.8%. When the content of the glutaraldehyde in the glutaraldehyde disinfection solution is not less than 2.0%, the color development speed rises. The method has the characteristics of high accuracy, obvious chromatic difference, rapid determination, convenient use and the like, and the chemical indicator composition is an ideal chemical concentration indicator of the glutaraldehyde disinfection solution.
Description
Technical field
The invention belongs to the disinfecting field, relate to a kind of chemical indicator composition of measuring glutaraldehyde concentration in the glutaral disinfectant solution.
Background technology
Glutaraldehyde has efficiently, wide spectrum, fast killing microorganisms effect, can effectively kill various bacterial propagules, tubercle bacillus, fungi, virus and bacterial spore.Glutaraldehyde water solution is extensively used the sterilization and the sterilization of medicine equipment, is to non-refractory, is afraid of the corrosion medicine equipment, as endoscope, anesthesia outfit, sterilization of lung ventilator pipeline and the first-selected sanitizer of sterilizing.The thimerosal that contains 2% glutaraldehyde is best working concentration, national health department regulation: new open the concentration of glutaraldehyde disinfectant that uses must not＜2.0%, the concentration of glutaraldehyde disinfectant in the use must not＜1.8%.In actual use, glutaraldehyde concentration in the glutaral disinfectant solution, can be because product be defective, use exceeds schedule time, the multiple reasons such as excess moisture that the apparatus that is sterilized or sterilizes is brought into, tend to make glutaraldehyde concentration decline in the thimerosal, whether continue again to use, just must measure at any time whether glutaraldehyde content meets minimum working concentration requirement in the glutaral disinfectant solution.This is for guaranteeing that the sterilization and the effect of sterilization are vital.
At present, measure concentration of glutaraldehyde disinfectant with the chemistry titration method, accurately and reliably, but step is cumbersome, time is long, the general quality control that just is used for lab investigation check and manufacturing enterprise's product, be not suitable for medical personnel and health and epidemic prevention personnel, concentration of glutaraldehyde disinfectant is carried out fast detecting, at present, more simple and rapid assay method is to use the glutaraldehyde concentration test card, as the glutaraldehyde concentration test card of Minnesota Mining and Manufacturing Company, and the glutaraldehyde concentration test card (patent publication No.: CN1477368A) of Wuhan Kang Weida health care product company limited and your health care product company limited production of Kaifeng Kang Jie.Its method is absorption sodium sulphite and a glycine solution on special test paper, is cut into blockage after the oven dry, is attached on the ground that does not absorb water with viscose glue then, sets off the black limit again around the indication blockage paper and rectifies.During test, test card is immersed in the glutaraldehyde solution, take out the back and inhale with thieving paper and removes unnecessary glutaraldehyde solution, observation test card change color in 5～8 minutes, evenly flavescence represents that glutaraldehyde concentration is up to standard, otherwise the indicating card flavescence is inhomogeneous or do not develop the color.Though this method test operation is more convenient, distinguish that the variable color accuracy still has than mistake.Simple and easy quick colorimetric method for determining concentration of glutaraldehyde disinfectant (health care equipped for 2001 the 3rd phases) by developments such as Dai Yong, be in disposable color comparison tube, splash into glutaraldehyde detection liquid with inhaling the sample dropper, splash into reagent A and B mixing more respectively, colour developing back and standard color solution colorimetric, if excessive concentration is measured by this method after the dilution again, colorimetric result is multiplied by extension rate again.The method and chemistry titration method compare, and maximum error can reach 20%, and be also inconvenient during actual the use.With chemistry titration and Instrument measuring method, it is operated all than more complicated, is not suitable for the fast measuring of concentration of glutaraldehyde disinfectant.
Therefore, research and develop a kind of simple and easy, quick, accurately detect that the indicator of glutaraldehyde concentration is in demand in the glutaral disinfectant solution.
Summary of the invention
Glutaraldehyde concentration chemical indicator composition in a kind of glutaral disinfectant solution of the present invention, it is packed into by sulphite, amino acid, buffer solution regulator and forms in the flint glass pipe of (plug) with cover or the lucite pipe.Its principle be quantitative sulphite (content is 1.8% the needed sulphite amount of glutaraldehyde complete reaction in the thimerosal) with glutaral disinfectant solution in the glutaraldehyde generation addition reaction of corresponding mol ratio, generate α-hydroxy sulfonate, its solution is colourless; If tested glutaral disinfectant solution glutaraldehyde content〉1.8%, then also have remaining glutaraldehyde in the solution, amino acid reacts with remaining glutaraldehyde again, and product can show yellow.Because sulphite, amino acid are different with the glutaraldehyde reaction velocity, the former reacts moment, the latter is slow slightly, and sulphite, buffer solution regulator solid are fine powdered, dissolution velocity is very fast in solution, amino acid is bigger solid crystal particle, and dissolution velocity is relatively slow in solution.Simultaneously, buffer solution regulator can be regulated the potential of hydrogen of keeping the testing liquid system, to guarantee the reliability and the repeatability of experiment; So glutaraldehyde quantitative in the tested thimerosal is earlier with the sulphite complete reaction of corresponding mol ratio, then thimerosal is colourless; If the glutaraldehyde content in the tested thimerosal〉1.8%, have only in thimerosal, to also have not participate in and react remaining glutaraldehyde with sulphite, it just can show yellow with the amino acid reaction, and glutaraldehyde content is high more, and its solution colour developing is fast more, yellow is dark more.Thereby whether can indicate in the glutaral disinfectant solution glutaraldehyde content＜1.8% or 〉=1.8%.When the glutaraldehyde content in the glutaral disinfectant solution 〉=2.0%, the solution developing time can be very fast.This indicator can be used for judging whether glutaral disinfectant solution meets minimum working concentration〉1.8% requirement.
During use, get a glutaraldehyde concentration indicator tubule, open loam cake (plug), by the operation instruction requirement, accurately add quantitative glutaral disinfectant solution with transfer pipet, 3～5min is left standstill in jolting gently, can observe the situation of glutaral disinfectant solution change color.Its solution is colourless substantially, then represents glutaraldehyde content＜1.8% in the tested glutaral disinfectant solution; If it is light yellow or yellow that solution demonstrates, then represent glutaraldehyde content 〉=1.8% in the glutaral disinfectant solution.When the glutaraldehyde content in the glutaral disinfectant solution 〉=2.0%, the solution colour developing obviously, developing time is fast.The method has characteristics such as accuracy height, aberration is obvious, judgement is quick, easy to use, is a kind of desirable concentration of glutaraldehyde disinfectant chemical indicator.
Embodiment
Mode below the objective of the invention is to lead to realizes.
Key of the present invention is to want each component addition in the strict control indicator, therefore, adopts the requirement of " quantitatively control, component is up to standard, sampling observation is qualified ".Earlier sulphite and buffer solution regulator are ground to form fine powder respectively, cross 60 mesh sieves, in every inditron, the sulphite amount of packing into is 0.1～0.5g.Buffer solution regulator, charge weight is 0.02～0.10g in every inditron.The crystallization of developer amino acid solid particle, in every inditron, charge weight is 0.2～1.0g.Three kinds of reagent are all packed in the same pipe, and lid lid (plug) seals and gets final product.
Random sampling detects, and sample size is no less than 5%.The preparation glutaraldehyde content is 1.6%, 1.8%, 2.0% test solution, get 6 coupons, the glutaral disinfectant solution test solution that accurately adds 3～10ml 1.6%, 1.8%, 2.0% respectively with transfer pipet, jolting gently, leave standstill 3～5min, observe the situation of glutaral disinfectant solution test solution change color, meet the quality standard requirement, can be packaged as finished product.
To sodium bisulfite, potassium bisulfite, sodium pyrosulfite, each 0.1～0.5g of potassium metabisulfite be housed respectively, each 0.2～1.0g of amino acid developer, the inditron of each 0.02～0.10g of buffer solution regulator, every kind each 30, put into 54 ℃ of constant temperature ovens more respectively 14 days, in 37 ℃ of constant temperature ovens 90 days, detect the stability of director composition then.Through experiment confirm, the stability of director composition has reached the standard of design code fully.
Specific embodiment is:
Embodiment 1 accurate weighing sodium bisulfite 0.1g, weighing amino acid developer 0.2g again, buffer solution regulator 0.02g, the capacity of packing in the lump are in the colourless transparent glass pipe with cover or lucite pipe of 5ml, friction top, seal, adorn box and get final product.During use, measure the dense 2.0～2.5ml of tested glutaral disinfectant solution, join in the indicator pipe, solution colour variation in the inditron was observed in jolting in 3～5 minutes, make judgement then and get final product.
Embodiment 2 accurate weighing potassium bisulfite 0.1g, weighing amino acid developer 0.2g again, buffer solution regulator 0.02g, the capacity of packing in the lump are in the colourless transparent glass pipe with cover or lucite pipe of 5ml, friction top, seal, adorn box and get final product.During use, measure the dense 2.0～2.5ml of tested glutaral disinfectant solution, join in the indicator pipe, solution colour variation in the inditron was observed in jolting in 3～5 minutes, make judgement then and get final product.
Embodiment 3 accurate weighing sodium pyrosulfite 0.1g, weighing amino acid developer 0.2g again, buffer solution regulator 0.02g, the capacity of packing in the lump are in the colourless transparent glass pipe with cover or lucite pipe of 5ml, friction top, seal, adorn box and get final product.During use, measure the dense 2.0～2.5ml of tested glutaral disinfectant solution, join in the indicator pipe, solution colour variation in the inditron was observed in jolting in 3～5 minutes, make judgement then and get final product.
Embodiment 4 accurate weighing potassium metabisulfite 0.1g, weighing amino acid developer 0.2g again, buffer solution regulator 0.02g, the capacity of packing in the lump are in the colourless transparent glass pipe with cover or lucite pipe of 5ml, friction top, seal, adorn box and get final product.During use, measure the dense 2.0～2.5ml of tested glutaral disinfectant solution, join in the indicator pipe, solution colour variation in the inditron was observed in jolting in 3～5 minutes, make judgement then and get final product.
Claims (5)
1, measuring the concentration of glutaraldehyde disinfectant director composition is made up of sulphite, amino acid, buffer solution regulator and indicator pipe.Glutaral disinfectant solution is added in the indicator pipe, glutaraldehyde promptly with indicator generation chemical reaction, demonstrate yellow colourless or that the depth is different, can indicate in the glutaral disinfectant solution glutaraldehyde content whether＜1.8% or 〉=1.8%.Be used to judge whether glutaral disinfectant solution meets the requirement of minimum working concentration 〉=1.8%.
2, sulphite described in claim 1 comprises sodium bisulfite, potassium bisulfite, sodium pyrosulfite, potassium metabisulfite.In every inditron, the sulphite amount of packing into is 0.1～0.5g.
3, amino acid described in claim 1 comprises glycocoll, alanine, Silk propylhomoserin, histidine, glutamic acid, tryptophane, threonine etc.In every inditron, the amount of amino acid of packing into is 0.2～1.0g.
4, buffer solution regulator described in claim 1 is meant that (sodium hydrogen phosphate: potassium dihydrogen phosphate=2.38:1.36), charge weight is 0.02～0.10g in every inditron for sodium hydrogen phosphate and potassium dihydrogen phosphate.
5, indicator pipe described in claim 1 is graduated colourless transparent glass pipe or lucite pipe with cover, and capacity is 3～10ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710195734XA CN101458216A (en) | 2007-12-14 | 2007-12-14 | Indicator composition for detection concentration of glutaraldehyde disinfectant |
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| CNA200710195734XA CN101458216A (en) | 2007-12-14 | 2007-12-14 | Indicator composition for detection concentration of glutaraldehyde disinfectant |
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| CN101458216A true CN101458216A (en) | 2009-06-17 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102967599A (en) * | 2012-11-19 | 2013-03-13 | 广州自远生物科技有限公司 | Detecting device for detecting alkali concentration of disinfectant at farm and using method of detecting device |
| CN105067545A (en) * | 2015-08-12 | 2015-11-18 | 广州市顺元医疗器械有限公司 | Disinfectant concentration detection method and concentration monitoring device using method |
| CN104024852B (en) * | 2012-01-31 | 2016-09-07 | Dfi株式会社 | Oxygen radical detection method in human body |
| CN111024687A (en) * | 2019-12-23 | 2020-04-17 | 广东环凯微生物科技有限公司 | Glutaraldehyde determination test paper and rapid determination method |
| CN112724964A (en) * | 2020-12-29 | 2021-04-30 | 优品保技术（北京）有限公司 | Disinfectant with indication and tracing functions and application thereof |
-
2007
- 2007-12-14 CN CNA200710195734XA patent/CN101458216A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104024852B (en) * | 2012-01-31 | 2016-09-07 | Dfi株式会社 | Oxygen radical detection method in human body |
| CN102967599A (en) * | 2012-11-19 | 2013-03-13 | 广州自远生物科技有限公司 | Detecting device for detecting alkali concentration of disinfectant at farm and using method of detecting device |
| CN102967599B (en) * | 2012-11-19 | 2016-01-13 | 广州自远生物科技有限公司 | A kind of pick-up unit for detecting plant's thimerosal alkali concn and using method thereof |
| CN105067545A (en) * | 2015-08-12 | 2015-11-18 | 广州市顺元医疗器械有限公司 | Disinfectant concentration detection method and concentration monitoring device using method |
| CN111024687A (en) * | 2019-12-23 | 2020-04-17 | 广东环凯微生物科技有限公司 | Glutaraldehyde determination test paper and rapid determination method |
| CN111024687B (en) * | 2019-12-23 | 2022-03-25 | 广东环凯微生物科技有限公司 | Glutaraldehyde determination test paper and rapid determination method |
| CN112724964A (en) * | 2020-12-29 | 2021-04-30 | 优品保技术（北京）有限公司 | Disinfectant with indication and tracing functions and application thereof |
| CN112724964B (en) * | 2020-12-29 | 2023-03-21 | 优品保技术（北京）有限公司 | Disinfectant with indication and tracing functions and application thereof |
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Open date: 20090617 |