CN105062889A - Cell volatile matter collecting incubator - Google Patents
Cell volatile matter collecting incubator Download PDFInfo
- Publication number
- CN105062889A CN105062889A CN201510459096.2A CN201510459096A CN105062889A CN 105062889 A CN105062889 A CN 105062889A CN 201510459096 A CN201510459096 A CN 201510459096A CN 105062889 A CN105062889 A CN 105062889A
- Authority
- CN
- China
- Prior art keywords
- beaker
- incubator
- cell
- culture dish
- volatile matter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/36—Means for collection or storage of gas; Gas holders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a cell volatile matter collecting incubator for disease biomarker research. The cell volatile matter collecting incubator comprises a culture dish which is used for accommodating a to-be-detected liquid therein; a long elbow and a short elbow, wherein the inner ends of both the long elbow and the short elbow are arranged inside the culture dish; a beaker which is buckled in the liquid of the culture dish in an inverted mode, wherein the inner end of the long elbow is higher than the surface of the liquid and is located at the top of the beaker while the inner end of the short elbow is lower than the surface of the liquid and is located at the bottom of the beaker; and a crystallizing dish which covers and is buckled on the beaker, wherein the outer ends of both the long elbow and the short elbow extend out of the crystallizing dish. The incubator disclosed by the invention is a simple and non-sealed device, which not only can guarantee gas exchange between cells inside and outside the beaker but also can cause relatively low influence on cell activity, so that collected cell volatile matters are more significant in research; therefore, the incubator is more conducive to the exploration of disease metabolic mechanism and is beneficial for the preliminary screening and the early diagnosis of tumors and like diseases.
Description
Technical field
The present invention relates to a kind of cell culture apparatus, particularly a kind of under non-closed environment for the cell culture apparatus of collecting cell volatile biomarkers thing.
Background technology
At present, the diagnosis of tumour needs histopathology, aspiration biopsy etc. to have wound inspection more, but these check that time-histories is longer, and especially traumatic operation is made troubles to patient and misery.For this reason, urgently develop a kind of simple, effectively, economic, convenient, noninvasive diagnostic techniques is for the early screening of disease and clinical diagnosis.In recent years, people are studied the volatile organic compounds that solid tumor produces in multiple, comprising the blood of tumour patient, urine, expired air, tumor tissues and tumour cell, attempting the novel method being used as disease early screening and diagnosis by finding volatility marker.Human body is that a complexity comprising multiple internal organs is individual, has multiple enzyme and metabolic system to participate in the metabolism of body, and the pathophysiological process of disease; And because affecting by factors such as diet, the volatility marker of researching human body body fluid and expired air is relatively complicated.For this reason, the volatility marker of analysis of disease will contribute to the possible metabolic mechanism probing into disease generation further on a cellular level.
At present, existing document discloses a kind of cell cultures for detecting cell volatile organic compounds and proofing unit, application glass culturing bottle is cultivated, but this device is stopping property, this kind of closed environment is unfavorable for that cell carries out gaseous interchange, suitable culture environment can not be provided for cell, easily cause the anoxia state of cell, apoptosis is caused to affect the eubolism of cell, the organic compound that under this kind of environment, cell volatilizes is not probably the material of metabolism output under cell normal condition to be detected, be unfavorable for tumor marker really cutting analyse, and it is not analyzed the activity of cell under this kind of culture environment and state, therefore the true and reliable property of experimental result needs to be investigated further.
Document is separately had also to disclose a kind of cell culture apparatus, for detecting the volatile organic compounds of cell, but this device complex structure, it directly need be connected with the gas tank containing 95%CO2 with 5%O2, and need the multiple accessory such as catalyzer, hydrocarbon trap, heat distribution formula mass flow controller, sterile filters, heating in water bath circuit, magnet, electronic control valve, dilution pipeline, therefore operation is relatively loaded down with trivial details, expense is also relatively high.
Summary of the invention
For solving the problems of the technologies described above, the object of the invention is to: provide a kind of cell volatile matter to collect incubator, this incubator is the device of easy, non-stopping property, not only ensure that the gaseous interchange of cell inside and outside beaker, and it is less on cytoactive impact, make the cell volatile matter collected more have Research Significance, be therefore more conducive to the metabolic mechanism probing into disease, be beneficial to preliminary examination and the early diagnosis of the diseases such as tumour.
For achieving the above object, the technical solution adopted in the present invention is: a kind of cell volatile matter collects incubator, and it comprises:
One culture dish, this culture dish inside is loaded with liquid to be detected;
One long bend pipe and a short bend pipe, the inner of this long bend pipe and short bend pipe is all placed in this culture dish;
One beaker, this beaker back-off is in the liquid of this culture dish and surround the inner of this long bend pipe and short bend pipe, wherein, the inner of this long bend pipe be positioned at this beaker top and higher than this fluid surface, the inner of this short bend pipe be positioned at this beaker bottom and lower than this fluid surface;
One crystallizing dish, this crystallizing dish cover is buckled on this beaker, all bending outside stretching out in this crystallizing dish, outer end of this long bend pipe and short bend pipe.
Described cell volatile matter collects incubator, and the opening of this crystallizing dish container bottom is lower than the opening on this culture dish top.
Described cell volatile matter collects incubator, and the liquid level in the beaker after this beaker back-off and the liquid level in this culture dish keep concordant.
Described cell volatile matter collects incubator, and this liquid to be detected is cell to be detected and substratum.
Described cell volatile matter collects incubator, and the outer end of this long bend pipe connects a collector by a silicone tube, and this collector and this silicone tube junction are provided with a knob, for the pressure equilibrium inside and outside collection and confinement of gases and maintenance beaker.
Described cell volatile matter collects incubator, and this silica gel pipe range 3cm, this collector is the Storage Time in Gas Collecting Bag of 0.5L.
Described cell volatile matter collects incubator, and the specification of this culture dish is 90mm, and the specification of this beaker is 250mL, and the specification of this crystallizing dish is 125mm.
Described cell volatile matter collects incubator, and the diameter of this beaker is less than the diameter of culture dish.
Described cell volatile matter collects incubator, and the height of this beaker is higher than the height of this culture dish.
Described cell volatile matter collects incubator, and the diameter of this crystallizing dish is greater than the diameter of this culture dish.
Compared with prior art, adopt the invention has the advantages that of technique scheme: the closed environment and the shortcoming such as complicated operation, accessory redundancy that instant invention overcomes cell cultures in prior art, provide a kind of easy, non-stopping property, the device of low cost, ensure that the gaseous interchange of cell inside and outside beaker, for cell provides relatively normal culture environment, by the apoptosis of flow cytomery cell, result showed cell cultivates activity after 24 hours in this device can reach 92.18% ± 1.26%.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Description of reference numerals: 1-culture dish; 2-testing liquid; The long bend pipe of 3-; The short bend pipe of 4-; 5-beaker; 6-crystallizing dish; 7-silicone tube; 8-collector; 81-knob.
Embodiment
Further describe the present invention below in conjunction with specific embodiments and the drawings, advantage and disadvantage of the present invention will be more clear along with description.
As shown in Figure 1, for cell volatile matter of the present invention collects the solid assembling schematic diagram of incubator, it comprises:
One culture dish 1, this culture dish 1 inside is loaded with liquid 2 to be detected; Wherein, in case study on implementation of the present invention, liquid 2 to be detected comprises cell to be detected and substratum, also can need to add corresponding medicine because of experimental design, the specification of this culture dish 1 is 90mm, in this culture dish 1, put into long bend pipe 3 and a short bend pipe 4, the inner of this long bend pipe 3 and short bend pipe 4 is all placed in this culture dish 1.
Again take a specification be beaker 5 back-off of 250ml in the liquid 2 of this culture dish 1, the liquid level in this beaker 5 will be guaranteed concordant with the liquid level in this culture dish 1.Wherein, the diameter of this beaker 5 is less than the diameter of this culture dish 1, but in the inner of this long bend pipe 3 and short bend pipe 4 will all be enclosed in by this beaker 5 simultaneously, to go for a stroll song in the bottom part ring of this beaker 5 in the inner of this long bend pipe 3, and to make upon bending bottom it higher than this liquid 2 surface and be stretched to the top of this beaker 5 always, the gas that long bend pipe 3 produces for collecting beaker inner cell, the inner of this short bend pipe 4 is positioned at the bottom of this beaker 5 and lower than this liquid 2 surface, short bend pipe 4 draws the experiment that testing liquid carries out next step at the end of testing.
Be that the crystallizing dish 6 of 125mm is buckled on this beaker 5 again by a specification, height due to beaker 5 is higher than the height of this culture dish 1, and the diameter of crystallizing dish 6 is greater than the diameter of culture dish 1, therefore when crystallizing dish 6 cover is buckled on beaker 5, certain distance is arranged at the bottom of the opening bottom container crystallizing dish 6 and culture dish 1, therefore, a circular space is formed between culture dish 1 and crystallizing dish 6, but the horizontal plane at the opening place of this crystallizing dish 6 container bottom will lower than the horizontal plane at the opening place on this culture dish 1 top, ensure testing liquid 2 in this culture dish 1 by the pollution of other materials in outside air, simultaneously, the then all bending outside stretching out in this crystallizing dish 6, outer end of this long bend pipe 3 and short bend pipe 4.
The outer end of this long bend pipe 3 connects a collector 8 by the silicone tube 7 of a long 3cm, and this collector 8 is provided with knob 81 with this silicone tube 7 junction, for the pressure equilibrium inside and outside collection and confinement of gases and maintenance beaker.In case study on implementation of the present invention, what collector 8 adopted is the Storage Time in Gas Collecting Bag of 0.5L.
Therefore learnt by above content, in apparatus of the present invention, culture dish 1 upper opening is non-closed state, simultaneously, the diameter of crystallizing dish 6 is greater than the diameter of culture dish 1, and certain distance is arranged at the bottom of opening bottom container crystallizing dish 6 and culture dish 1, therefore, a circular space is formed between culture dish 1 and crystallizing dish 6, therefore, cell in culture dish 1 can carry out gaseous interchange with the cell in beaker 5 under non-closed environment, the gas produced is placed in the top of beaker 5, long bend pipe 3 collects gas by collector 8, volatile matter after collection carries out other metabonomic analysis such as solid-phase microextraction and GC/MS Analysis further, short bend pipe 4 is for extracting cell in order to further testing inspection.As apoptosis, PCR etc.
Simultaneously, instant invention overcomes closed environment and the shortcoming such as complicated operation, accessory redundancy of cell cultures in prior art, provide a kind of easy, non-stopping property, the device of low cost, ensure that the gaseous interchange of cell inside and outside beaker 5, less on cytoactive impact, for cell provides relatively normal culture environment, by the apoptosis of flow cytomery cell, result showed cell cultivates activity after 24 hours in this device can reach 92.18% ± 1.26%; Therefore the organic compound making cell volatilize is more genuine and believable, is therefore more conducive to the metabolic mechanism probing into disease, is beneficial to preliminary examination and the early diagnosis of disease.
Above explanation and embodiment are only exemplary, do not form any restriction to scope of the present invention.It will be understood by those skilled in the art that and can modify to the details of technical solution of the present invention and form or replace down without departing from the spirit and scope of the present invention, but these amendments and replacement all fall within the scope of protection of the present invention.
Claims (10)
1. cell volatile matter collects an incubator, and it is characterized in that, it comprises:
One culture dish, this culture dish inside is loaded with liquid to be detected;
One long bend pipe and a short bend pipe, the inner of this long bend pipe and short bend pipe is all placed in this culture dish;
One beaker, this beaker back-off is in the liquid of this culture dish and surround the inner of this long bend pipe and short bend pipe, wherein, the inner of this long bend pipe be positioned at this beaker top and higher than this fluid surface, the inner of this short bend pipe be positioned at this beaker bottom and lower than this fluid surface;
One crystallizing dish, this crystallizing dish cover is buckled on this beaker, all bending outside stretching out in this crystallizing dish, outer end of this long bend pipe and short bend pipe.
2. cell volatile matter according to claim 1 collects incubator, it is characterized in that, the opening of this crystallizing dish container bottom is lower than the opening on this culture dish top.
3. cell volatile matter according to claim 1 collects incubator, and it is characterized in that, the liquid level in the beaker after this beaker back-off and the liquid level in this culture dish keep concordant.
4. cell volatile matter according to claim 1 collects incubator, and it is characterized in that, this liquid to be detected is cell to be detected and substratum.
5. cell volatile matter according to claim 1 collects incubator, it is characterized in that, the outer end of this long bend pipe connects a collector by a silicone tube, and this collector and this silicone tube junction are provided with knob, for the pressure equilibrium inside and outside collection and confinement of gases and maintenance beaker.
6. cell volatile matter according to claim 5 collects incubator, and it is characterized in that, this silica gel pipe range 3cm, this collector is the Storage Time in Gas Collecting Bag of 0.5L.
7. cell volatile matter according to claim 1 collects incubator, and it is characterized in that, the specification of this culture dish is 90mm, and the specification of this beaker is 250mL, and the specification of this crystallizing dish is 125mm.
8. cell volatile matter according to claim 1 collects incubator, and it is characterized in that, the diameter of this beaker is less than the diameter of culture dish.
9. cell volatile matter according to claim 1 collects incubator, it is characterized in that, the height of this beaker is higher than the height of this culture dish.
10. cell volatile matter according to claim 1 collects incubator, and it is characterized in that, the diameter of this crystallizing dish is greater than the diameter of this culture dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510459096.2A CN105062889B (en) | 2015-07-30 | 2015-07-30 | A kind of cell volatile matter collects incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510459096.2A CN105062889B (en) | 2015-07-30 | 2015-07-30 | A kind of cell volatile matter collects incubator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105062889A true CN105062889A (en) | 2015-11-18 |
| CN105062889B CN105062889B (en) | 2017-11-17 |
Family
ID=54492410
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510459096.2A Active CN105062889B (en) | 2015-07-30 | 2015-07-30 | A kind of cell volatile matter collects incubator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105062889B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994028501A1 (en) * | 1993-05-24 | 1994-12-08 | Cornell Research Foundation, Inc. | Automated, multicompartmental cell culture system |
| CN102089420A (en) * | 2008-07-08 | 2011-06-08 | 威尔森沃尔夫制造公司 | Improved gas permeable cell culture device and method of use |
| CN202886148U (en) * | 2012-08-09 | 2013-04-17 | 山东省产品质量监督检验研究院 | Sampling collector of organic volatile matters in product/sample |
| CN203613193U (en) * | 2013-10-23 | 2014-05-28 | 上海交通大学医学院附属仁济医院 | Dynamic detection device for metabolism and respiration of cells |
-
2015
- 2015-07-30 CN CN201510459096.2A patent/CN105062889B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994028501A1 (en) * | 1993-05-24 | 1994-12-08 | Cornell Research Foundation, Inc. | Automated, multicompartmental cell culture system |
| CN102089420A (en) * | 2008-07-08 | 2011-06-08 | 威尔森沃尔夫制造公司 | Improved gas permeable cell culture device and method of use |
| CN202886148U (en) * | 2012-08-09 | 2013-04-17 | 山东省产品质量监督检验研究院 | Sampling collector of organic volatile matters in product/sample |
| CN203613193U (en) * | 2013-10-23 | 2014-05-28 | 上海交通大学医学院附属仁济医院 | Dynamic detection device for metabolism and respiration of cells |
Non-Patent Citations (1)
| Title |
|---|
| 秦伟主编: "《初中生物学教科书经典教学实验介评》", 28 February 2014 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105062889B (en) | 2017-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103146575B (en) | Small-scale carbon dioxide cell cultivating cabinet with fluid interface and electrical interface | |
| CN202119641U (en) | Sampling tube of excrement analysis system | |
| CN205105947U (en) | Mice experimental observation case | |
| CN204008229U (en) | A kind of segmentation urine collecting device | |
| CN105062889A (en) | Cell volatile matter collecting incubator | |
| CN206229413U (en) | A kind of Blood specimen collection rack for test tube | |
| CN201275151Y (en) | Barrow type special vehicle for inspecting sample | |
| CN201535722U (en) | Telescopic stool sampler | |
| CN201242514Y (en) | Sampler | |
| CN209450563U (en) | Seminal fluid collecting device | |
| CN203089219U (en) | Sample storage device for tumor needle biopsy | |
| CN208081875U (en) | A kind of separate type evaporator for bio-pharmaceuticals | |
| CN202342092U (en) | Device for cutting and collecting samples under endoscope | |
| CN203269947U (en) | Miniature carbon dioxide cell incubator with fluid pipe interface and electric interface | |
| CN207640710U (en) | Novel thoracic drainage bottle | |
| CN206359528U (en) | Blood platelet RNA real-time fluorescence PCR cancer diagnosing kits | |
| CN203820790U (en) | Inspection tube for counting gas-producingmicroorganisms | |
| CN202420939U (en) | Novel immunohistochemical wet box | |
| CN104555010A (en) | Medical sample submission box | |
| CN201575936U (en) | Device for conveniently receiving and sampling stool | |
| CN201709222U (en) | Dynamic and static dual-purposes metabonomics experimental cage for animals | |
| CN205288495U (en) | Collection device of blood sampling test tube | |
| CN215541100U (en) | Multifunctional experiment platform for clinical examination | |
| CN204373960U (en) | A kind of inspection emiction sampling cup | |
| CN203794903U (en) | Cell hypoxia culture device capable of being continuously observed under microscope |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Zhuo Inventor after: Shi Ce Inventor after: Li Dandan Inventor after: Zhao Yanqiu Inventor after: Wei Hong Inventor after: Zhou Jin Inventor before: Li Dandan Inventor before: Zhou Jin Inventor before: Zhang Zhuo |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |