CN105053451B - Pan work containing sea cucumber and saussurea involucrata ingredient - Google Patents
Pan work containing sea cucumber and saussurea involucrata ingredient Download PDFInfo
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- CN105053451B CN105053451B CN201510468531.8A CN201510468531A CN105053451B CN 105053451 B CN105053451 B CN 105053451B CN 201510468531 A CN201510468531 A CN 201510468531A CN 105053451 B CN105053451 B CN 105053451B
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Abstract
The present invention discloses a kind of pan work containing sea cucumber and saussurea involucrata ingredient significantly improving immunity of organisms, radioresistance and antioxidation, it is characterised in that is made of following component and mass percent：Sea cucumber egg powder 8%, Saussurea involucrata culture powder 2.5%, cocoa power 7.5%, xylo-oligosaccharide powder 10%, skimmed milk power 5%, D mannitols 54.5%, Cranberry fruit powder 12%, strawberry essence 0.1%, flavoring orange essence 0.1%, magnesium stearate 0.3%；The sea cucumber egg powder is prepared as follows：New fresh sea cucumber is dissected and takes out gonad, isolates sea cucumber ovary；Sea cucumber ovary crushing grinding is slurried；At 50 DEG C, the papain of the flavor protease and mass percent 3% of mass percent 3% is added, hydrolyzes 1.5 hours；It is lyophilized after enzymolysis liquid is concentrated, is crushed into sea cucumber egg powder.
Description
Background technology
The invention belongs to casual health food, especially one kind can significantly improving immunity of organisms, radioresistance and anti-oxidant
The pan work containing sea cucumber and saussurea involucrata ingredient of effect.
Background technology
Sea cucumber protein is higher（It is especially enriched in collagen）, low fat, rich in various amino acid needed by human, dimension life
Element, essential fatty acid and macro- and trace-element are traditional excellent tonic products.Saussurea involucrata is the distinctive rare Chinese herbal medicine in Xinjiang,
Energy invigorating kidney, promoting blood circulation, strengthening the bones and muscles, trophic nerve, dysregulation body fluid.Saussurea involucrata culture be aseptically, it is that saussurea involucrata is in vitro
Plant organ, tissue, cell, embryo or Protoplast cuhnre are on the culture medium manually prepared, under conditions of manual control
Carry out the product of culture growth.Containing with active constituent similar in natural snow lotus and with the comparable pharmacological activity of natural snow lotus.
Candy is the favorite food of people institute, various in style, there is hard, butterfat, gel, matrix, inflation, tabletting, sandwich
And crisp-fried etc., but previous candy has the less of efficient nourishing and health preserving function.In order to solve this problem, number of patent application is
03112126.8 Chinese invention patent application discloses a kind of " sea cucumber candy and production method ", is " using sea cucumber as raw material system
Active sea cucumbers liquid is taken, isomalto-oligosaccharide powder, Isomalto-oligsaccharide and extracts of Chinese herbal medicine are equipped with, through reconciliation, infusion, is stirred
It mixes, cool down, moulding process is made ".
Its active sea cucumbers liquid is to filter sea cucumber by broken, defibrination, degradation, deodorant and obtain, disclosed Chinese herbal medicine
There are three types of formulas for extracting solution：The fruit of Chinese wolfberry, hawthorn, chrysanthemum, Siraitia grosvenorii；It is Schisandra chinensis, the fruit of Chinese wolfberry, plantain seed, white bud root, chrysanthemum, sweet
Grass, STEVIA REBAUDIANA；Chrysanthemum, honeysuckle, licorice, the sterculia seed, peppermint, STEVIA REBAUDIANA.There are the problem of it is as follows：
1. deodorant also filters out sea cucumber part nutritional ingredient while filtering, the nutritive value of sea cucumber is reduced；
2. the flavour of a drug of extracts of Chinese herbal medicine influence mouthfeel, " making candy sugar-free sweet taste ", the meaning of candy is lost；
3. not significantly improving immunity of organisms, radioresistance and oxidation resistant function and effect.
Invention content
The present invention is to provide one kind in order to solve the above-mentioned technical problem present in the prior art and can significantly improve body and exempt from
The pan work containing sea cucumber and saussurea involucrata ingredient of epidemic disease power, radioresistance and antioxidation.
Technical solution of the invention is：A kind of pan work containing sea cucumber and saussurea involucrata ingredient, it is characterised in that by
Following component and mass percent are constituted：Sea cucumber egg powder 8%, Saussurea involucrata culture powder 2.5%, cocoa power 7.5%, xylo-oligosaccharide powder
10%, skimmed milk power 5%, D-mannital 54.5%, Cranberry fruit powder 12%, strawberry essence 0.1%, flavoring orange essence 0.1%, stearic acid
Magnesium 0.3%；
The sea cucumber egg powder is prepared as follows：
A. new fresh sea cucumber is dissected and takes out gonad, isolate sea cucumber ovary；
B. sea cucumber ovary crushing grinding is slurried；
C. at 50 DEG C, the papain of the flavor protease and mass percent 3% of mass percent 3%, water is added
Solution 1.5 hours；
D. it is lyophilized after enzymolysis liquid being concentrated, is crushed into sea cucumber egg powder.
Compared with prior art, the present invention having the following advantages that：
1. each component interaction of the present invention, especially carries out sea cucumber egg powder with Saussurea involucrata culture powder to scale
Compounding, can significantly improve immunity of organisms, radioresistance and antioxidation；
2. sea cucumber egg powder belongs to animality（Cool property）, and Saussurea involucrata culture powder belongs to vegetalitas（Warm nature）, the two is dynamic to plant knot
It closes, the cool complementation of temperature, complements each other, suitable population is more extensive；
3. rational component can effectively remove the fishy smell of sea cucumber egg powder, candy fragrant taste is sweet, without to sea cucumber ovary
Deodorant filtration treatment is carried out, sea cucumber nutrient ingredients from lossing is avoided.
Specific implementation mode
First it is prepared as follows sea cucumber egg powder：
A. new fresh sea cucumber is dissected and takes out gonad, isolate sea cucumber ovary；
B. sea cucumber ovary crushing grinding is slurried；
C. at 50 DEG C, the papain of the flavor protease and mass percent 3% of mass percent 3%, water is added
Solution 1.5 hours；
D. it is lyophilized after enzymolysis liquid being concentrated, is crushed into sea cucumber egg powder.
Take sea cucumber egg powder 8% by mass percentage, Saussurea involucrata culture powder 2.5%, cocoa power 7.5%, xylo-oligosaccharide powder 10% takes off
Fat milk powder 5%, D-mannital 54.5%, Cranberry fruit powder 12%, strawberry essence 0.1%, flavoring orange essence 0.1%, magnesium stearate 0.3%
It is matched.It using existing production candy craft, is sufficiently mixed with three-dimensional mixer, the candies such as hard, tabletting is made.
Except sea cucumber egg powder, other raw materials are outsourcing, and specific manufacturer is as follows：
Experiment process：
SPF grades of healthy adult male Kunming strain mices 60 are selected, weight is 20 ~ 25 grams or so, random to be grouped, setting four
A processing.Processing 1 is control group：Feed 0.9% sodium chloride solution；Processing 2 feeds the solution of following component（Mass percent）：
Sea cucumber egg powder 10.5%, cocoa power 7.5%, xylo-oligosaccharide powder 10%, skimmed milk power 5%, D-mannital 54.5%, Cranberry fruit powder
12%, strawberry essence 0.1%, flavoring orange essence 0.1%, magnesium stearate 0.3%；Processing 3 feeds the solution of following component（Quality percentage
Than）：Saussurea involucrata culture powder 10.5%, cocoa power 7.5%, xylo-oligosaccharide powder 10%, skimmed milk power 5%, D-mannital 54.5% are climing
More certain kind of berries fruit powder 12%, strawberry essence 0.1%, flavoring orange essence 0.1%, magnesium stearate 0.3%；Processing 4 is fed described in the embodiment of the present invention
The solution of component.It feeds 1 time, feeds altogether 30 days according to the 0.2ml/10g of weight daily, then carry out strengthen immunity, resist
Oxidation and radioresistance experiment sampling.
Immunity is tested：
After experiment, pluck eyeball and take blood, the whole blood of acquisition is allowed to stand at room temperature for 30 minutes, then 4 DEG C,
5min is centrifuged under 4000rpm, detaches serum to measure total number born in blood（SOD）Activity and immunoglobulin
IgG activity is evaluating immune effect.Superoxide dismutase（SOD）The oxidative and anti-oxidative balance of body is played heavy to closing
The effect wanted, it can remove ultra-oxygen anion free radical（O2-）Protect cells from damage.IgG plays activating complement, neutralizes more
The effect of kind toxin, while it is also the important indicator for reacting immune status.
SOD activity is built up Bioengineering Research Institute's kit measurement using Nanjing and is measured using xanthine oxidase.Blood
IgG content is measured using Zhejiang Province Yilikang Bioisystech Co., Ltd's kit immunoturbidimetry in clear.Concrete outcome such as table 1.
1 sea cucumber of table and saussurea involucrata ingredient Different adding amount handle the influence to mouse immunity
From table 1 it follows that being individually added to SOD activity in the mice serum of sea cucumber egg powder and Saussurea involucrata culture solution
It is significantly higher than un-added control group with IgG content（P<0.05）, and sea cucumber egg powder and Saussurea involucrata culture solution are added simultaneously
SOD activity is significantly higher than addition single component group and control group in mice serum（P<0.05）, while adding sea cucumber egg powder and snow
IgG content is significantly higher than control group in the mice serum of lotus culture solution（P<0.05）, and have than single addition group raised
Trend.Illustrate that the compounding of sea cucumber of embodiment of the present invention egg powder and Saussurea involucrata culture can significantly improve the immunity of mouse.
Anti-oxidant experiment：
After experiment, pluck eyeball and take blood, the whole blood of acquisition is allowed to stand at room temperature for 30 minutes, then 4 DEG C,
5min is centrifuged under 4000rpm, detaches serum to measure catalase in blood（CAT）Activity and glutathione peroxidase
Enzyme（GSH-Px）Activity is evaluating antioxidant effect.Hydrogen peroxide be generated in oxidase catalyzed redox reaction it is thin
Cytotoxic substance, and the effect of catalase is the decomposition of catalyzing hydrogen peroxide, can shield to cell, cell is made to exempt from
It is endangered by hydrogen peroxide.Glutathione peroxidase（GSH-Px）It is a kind of important peroxidating being widely present in body
Object catabolic enzyme, it can make toxic peroxide be reduced into nontoxic hydroxy compounds, to protect the structure and work(of cell membrane
It can not be by the interference and damage of peroxide.
CAT and GSP-Px activity builds up Bioengineering Research Institute's kit measurement using Nanjing.Concrete outcome such as table 2.
2 sea cucumber of table and saussurea involucrata ingredient Different adding amount handle the influence to Antioxidation Effect
From Table 2, it can be seen that add simultaneously in the mice serum of sea cucumber egg powder and Saussurea involucrata culture solution CAT activity and
GSH-Px activity is significantly higher than the un-added control group of single component group（P<0.05）.Illustrate sea cucumber of embodiment of the present invention egg powder and
The compounding of Saussurea involucrata culture can significantly improve the anti-oxidation function of mouse.
Radioresistance is tested：
After feeding experiment, mono- ray radiation sources of 60Co Y, 100 cm of wave source distance, dosage rate is 1.0 Gy/
Min takes mouse tail blood respectively after radiating 5 days and 15 days, measures peripheral white blood cell with conventional microscopic counting, calculates 10
Leucocyte rise number in it, as evaluation mouse radioresistance index.Concrete outcome such as table 3.
3 sea cucumber of table and saussurea involucrata ingredient Different adding amount handle the influence to mouse radiation resistance
From table 3 it is observed that the mouse for being individually added to sea cucumber egg powder and Saussurea involucrata culture solution radiates 5 days and 10 days
Afterwards, quantity of leucocyte and adds the mouse radiation 5 of sea cucumber egg powder and Saussurea involucrata culture solution simultaneously than the notable raising of control group
, with after 10 days, quantity of leucocyte is significantly higher than addition single component group and control group for it（P<0.05）.In addition, adding sea cucumber simultaneously
The mouse of egg powder and Saussurea involucrata culture solution radiates the 5th day to the 10th day, and leucocyte rise number is significantly higher than control group（P< 0.05）, and have raised trend than single addition group.Illustrate the compounding energy of sea cucumber of embodiment of the present invention egg powder and Saussurea involucrata culture
Significantly improve the capability of resistance to radiation of mouse.
Claims (1)
1. a kind of pan work containing sea cucumber and saussurea involucrata ingredient, it is characterised in that be made of following component and mass percent：
Sea cucumber egg powder 8%, Saussurea involucrata culture powder 2.5%, cocoa power 7.5%, xylo-oligosaccharide powder 10%, skimmed milk power 5%, D-mannital
54.5%, Cranberry fruit powder 12%, strawberry essence 0.1%, flavoring orange essence 0.1%, magnesium stearate 0.3%；
The sea cucumber egg powder is prepared as follows：
A. new fresh sea cucumber is dissected and takes out gonad, isolate sea cucumber ovary；
B. sea cucumber ovary crushing grinding is slurried；
C. at 50 DEG C, the papain of the flavor protease and mass percent 3% of mass percent 3%, hydrolysis 1.5 is added
Hour；
D. it is lyophilized after enzymolysis liquid being concentrated, is crushed into sea cucumber egg powder.
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| CN201510468531.8A CN105053451B (en) | 2015-08-04 | 2015-08-04 | Pan work containing sea cucumber and saussurea involucrata ingredient |
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| CN201510468531.8A CN105053451B (en) | 2015-08-04 | 2015-08-04 | Pan work containing sea cucumber and saussurea involucrata ingredient |
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| CN105053451A CN105053451A (en) | 2015-11-18 |
| CN105053451B true CN105053451B (en) | 2018-09-04 |
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Families Citing this family (4)
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| CN107296142A (en) * | 2017-06-15 | 2017-10-27 | 谭文成 | A kind of preparation method of truffle saussurea involucrata pressed candy |
| CN107647324A (en) * | 2017-11-06 | 2018-02-02 | 刘海涛 | A kind of sea cucumber Composite Milk and preparation method thereof |
| CN109601878A (en) * | 2018-08-07 | 2019-04-12 | 丽睿客信息科技（北京）有限公司 | For protecting the nutraceutical of injury of lungs |
| CN112890176A (en) * | 2021-02-23 | 2021-06-04 | 山东承锦海洋生物科技有限公司 | Sea cucumber enzyme containing cordyceps militaris saussurea involucrata culture and preparation method thereof |
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| CN1173290A (en) * | 1996-08-12 | 1998-02-18 | 丁存义 | Health tea |
| CN101664210B (en) * | 2009-09-29 | 2012-05-23 | 山东省海洋水产研究所 | Processing method of sea cucumber egg nutrition |
| CN102406112A (en) * | 2011-11-30 | 2012-04-11 | 山东大学 | Nourishing health-care buccal tablet |
| CN104509884A (en) * | 2013-09-26 | 2015-04-15 | 修运庆 | Sea cucumber powder |
| CN103636909A (en) * | 2013-12-26 | 2014-03-19 | 深圳市聚特佳生物科技有限公司 | Low-sugar fruity mint tabletting candy and preparation method thereof |
| CN104256607A (en) * | 2014-10-11 | 2015-01-07 | 威海力元海洋生物科技有限公司 | Sea cucumber and saussurea involucrate healthcare product |
| CN104489209A (en) * | 2015-01-06 | 2015-04-08 | 韩山师范学院 | Oral sucrose-free squashed candy and preparation method of oral sucrose-free squashed candy |
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