CN105052839A - Artificial insemination instrument for queen bees with precision three-dimensional guide rail and using method thereof - Google Patents
Artificial insemination instrument for queen bees with precision three-dimensional guide rail and using method thereof Download PDFInfo
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- CN105052839A CN105052839A CN201510551609.2A CN201510551609A CN105052839A CN 105052839 A CN105052839 A CN 105052839A CN 201510551609 A CN201510551609 A CN 201510551609A CN 105052839 A CN105052839 A CN 105052839A
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- guide rail
- queen bee
- column
- artificial insemination
- precision
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- 230000009027 insemination Effects 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 12
- 241000257303 Hymenoptera Species 0.000 title abstract 5
- 230000003444 anaesthetic effect Effects 0.000 claims abstract description 23
- 239000004698 Polyethylene Substances 0.000 claims abstract description 19
- 229920000573 polyethylene Polymers 0.000 claims abstract description 19
- -1 polyethylene Polymers 0.000 claims abstract description 18
- 210000001015 abdomen Anatomy 0.000 claims description 16
- 210000000582 semen Anatomy 0.000 claims description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 5
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 3
- 239000001569 carbon dioxide Substances 0.000 claims description 3
- 210000003101 oviduct Anatomy 0.000 claims description 3
- 230000001737 promoting effect Effects 0.000 claims description 3
- 241000256844 Apis mellifera Species 0.000 abstract description 40
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 229910000831 Steel Inorganic materials 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 239000010959 steel Substances 0.000 description 2
- 241000256837 Apidae Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an artificial insemination instrument for queen bees with a precision three-dimensional guide rail and a using method thereof, and belongs to the professional technical field of honeybee breeding. The artificial insemination instrument comprises a ventral hook control rod, a back hook control rod, a microsyringe, a polyethylene syringe needle, upper and lower guide rails, front and rear guide rails, left and right guide rails, a spherical steering valve I, a magnetic type anesthetic room, a stand column I, a base, an anti-skid pad, a stand column II and a spherical steering valve II. The stand column I and the stand column II are arranged on the left side and the right side of the upper portion of the base respectively. The ventral hook control rod and the stand column I are connected through the spherical steering valve I arranged on the stand column I, the back hook control rod and the stand column II are connected through the spherical steering valve II arranged on the stand column II, the upper and lower guide rails, the front and rear guide rails and the left and right guide rails are combined into the three-dimensional guide rail, the three-dimensional guide rail is arranged on the stand column II, and the microsyringe is fixedly arranged on the upper and lower guide rails. According to the artificial insemination instrument for queen bees, on the premise of not damaging queen bees, artificial insemination of the queen bees can be precisely completed, the insemination precision is improved, operation is convenient, and the service life is prolonged.
Description
Technical field
The invention belongs to honeybee breeding professional skill field, particularly relate to one and test-tube instrument is carried out to virgin.
Background technology
Honeybee artificial insemination instrument carries out heredity and breeding of honey bees to study one of most important instrument, this instrument not only can carry out the researchs such as honeybee single male insemination, the how female insemination of Dan Xiong, autologous insemination, and plays a significant role in the purification of bee species (being), seed selection, conservation.McKesson HBOC's formula artificial insemination instrument for queen bee is the model of modern artificial insemination instrument for queen bee device, and it was sat the end of by, control stick fixed leg, abdomen hook control stick, back of the body hook control stick, syringe, the compositions such as queen bee anaesthetic room.The extensive use of the present world be the insemination instrument that is prototype with McKesson HBOC's formula, but the abdomen hook control stick of existing insemination instrument, back of the body hook control stick, syringe are all adopt shell fragment pressure fixing on control stick fixed leg, displacement accuracy is poor, and can only move forward and backward, manipulation space is limited.Insemination syringe needle adopts glass material to make, and glass crisp fritter is easily broken.Queen bee anaesthetic room is also by shell fragment pressure fixing base, picks and places inconvenience, and adjustment angle effort.
Therefore need badly in the middle of prior art and want a kind of novel technical scheme to solve this problem.
Summary of the invention
Technical problem to be solved by this invention is: provide a kind of artificial insemination instrument for queen bee with precision three-dimensional guide rail and using method thereof, when not injuring queen bee, the insemination to queen bee can be completed accurately, improving insemination precision, and easy to operate, service life increases.
A kind of artificial insemination instrument for queen bee with precision three-dimensional guide rail, it is characterized in that: comprise abdomen hook control stick, back of the body hook control stick, micro syringe, polyethylene syringe needle, upper/lower guide, front and back guide rail, left and right rail, spherical switching valve, magnetic-type anaesthetic room, column I, base, slipmat, column II and spherical switching valve II, the outer ring of described magnetic-type anaesthetic room is provided with iron ring, and is connected with base by the magnet that base side is arranged; Described column I and column II are separately positioned on the left and right sides on base top, and described abdomen hook control stick is connected by the spherical switching valve I be arranged on column I with column I; Described back of the body hook control stick is connected by the spherical switching valve II be arranged on column II with column II; Described upper/lower guide, front and back guide rail and left and right rail are combined into three-dimensional guide rail, and three-dimensional guide rail is arranged on column II, and the design of upper/lower guide, front and back guide rail and left and right rail application helical teeth is interconnected; Described polyethylene syringe needle is connected with micro syringe; Described micro syringe is fixedly installed on upper/lower guide; Described slipmat is arranged on four legs of base by height set screw.
With a using method for the artificial insemination instrument for queen bee of precision three-dimensional guide rail, it is characterized in that: comprise the following steps,
Step one, press with finger drone belly and force its adeagus to turn up, under drone adeagus is placed in polyethylene syringe needle, needle point is close to seminal fluid surface, pulls the screw rod of micro syringe to suck seminal fluid;
Step 2, queen bee is put into magnetic-type anaesthetic room, by the lower end of magnetic-type anaesthetic room access carbon dioxide;
Step 3, the afterbody of queen bee is placed in the upper end of magnetic-type anaesthetic room, utilizes the back of the body hook on the abdomen hook on abdomen hook control stick and back of the body hook control stick to be pulled open in the sting chamber of queen bee;
Step 4, adjustment upper/lower guide, front and back guide rail and left and right rail, enter the introitus of queen bee by the needle point of polyethylene syringe needle, the drone seminal fluid in syringe needle injects in the oviduct of queen bee by the screw rod promoting micro syringe, and queen bee artificial insemination completes.
Described polyethylene syringe needle is transparent elastic syringe needle, and needle point is conical.
Described upper/lower guide, front and back guide rail and left and right rail are connected by tooth bar, gear and dovetail groove.
By above-mentioned design, the present invention can bring following beneficial effect: a kind of artificial insemination instrument for queen bee with precision three-dimensional guide rail and using method thereof, when not injuring queen bee, the insemination to queen bee can be completed accurately, improve insemination precision, and easy to operate, service life increases.
The whole syringe needle of polyethylene insemination syringe needle is transparent, clearly can see seminal fluid situation of movement; And not easily ring is damaged in fragmentation, needle point is flexible, not easily stabs queen bee; Conically, during insemination, seminal fluid is not excessive for needle point.
Micro syringe main body adopts pmma material to make, and its advantage can inject sperm volume by accurate assurance, and the push rod of micro syringe, needle stand, syringe needle are all that stainless steel is made, and cleaning and sterilizing is convenient.
Ball-type switching valve adopts hard steel to make, and can carry out wide-angle movement on a large scale, and direction is adjustable, is easy to fixing, durable wear.
Upper/lower guide, front and back guide rail, left and right rail form the three-dimensional guide rail that can move freely, this component application precision three-dimensional guide rail technology, it is mobile that syringe can carry out top to bottom, left and right, front and rear three-dimensional, under the precision-fit of tooth bar, gear, dovetail groove, injection needle displacement accuracy reach 0.1mm, injection needle can be finely tuned accurately and conveniently to suitable injection angles, make the feeding queen bee vagina that injection needle safety is steady accurate, improve the precision of insemination, alleviate the injury to queen bee.
Queen bee anaesthetic room adopts magnetic-type design, when do for a large amount of queen bee inseminate time, one hand can pick and place queen bee anaesthetic room, very convenient.
The height set screw that base is arranged can realize the overall adjustment just of instrument of inseminating, and slipmat can prevent the movement of instrument at smooth surface of inseminating.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further illustrated:
Fig. 1 is a kind of artificial insemination instrument for queen bee structural representation with precision three-dimensional guide rail of the present invention.
Fig. 2 is a kind of artificial insemination instrument for queen bee using method schematic process flow diagram with precision three-dimensional guide rail of the present invention.
In figure, 1-abdomen hook control stick, 2-carry on the back guide rail, 7-left and right rail, the spherical switching valve of 8-, the magnetic-type anaesthetic room of 9-, 10-column I, 11-base, 12-slipmat, 13-column II, the spherical switching valve II of 14-before and after hook control stick, 3-micro syringe, 4-polyethylene syringe needle, 5-upper/lower guide, 6-.
Embodiment
A kind of artificial insemination instrument for queen bee with precision three-dimensional guide rail and using method thereof, as shown in Figure 1, comprise abdomen hook control stick 1, back of the body hook control stick 2, micro syringe 3, polyethylene syringe needle 4, upper/lower guide 5, front and back guide rail 6, left and right rail 7, spherical switching valve 8, magnetic-type anaesthetic room 9, column I 10, base 11, slipmat 12, column II 13 and spherical switching valve II 14, the outer ring of described magnetic-type anaesthetic room 9 is provided with iron ring, and is connected with base 11 by the magnet that base 11 side is arranged; Described column I 10 and column II 13 are separately positioned on the left and right sides on base 11 top, and described abdomen hook control stick 1 is connected by the spherical switching valve I 8 be arranged on column I 10 with column I 10; Described back of the body hook control stick 2 is connected by the spherical switching valve II 14 be arranged on column II 13 with column II 13; Described upper/lower guide 5, front and back guide rail 6 and left and right rail 7 are combined into three-dimensional guide rail, and three-dimensional guide rail is arranged on column II 13, and upper/lower guide 5, front and back guide rail 6 and left and right rail 7 are applied helical teeth design and be interconnected; Described polyethylene syringe needle 4 is connected with micro syringe 3; Described micro syringe 3 is fixedly installed on upper/lower guide 5; Described slipmat 12 is arranged on four legs of base 11 by height set screw.
Polyethylene syringe needle 4 adopts transparent polyethylene plastic processing to form.The needle point external diameter 0.26mm of syringe needle, needle point internal diameter 0.24mm.Syringe needle is elongated, slightly elasticity, and needle point conically.
Micro syringe 3 adopts 100 μ l microsyringes of customization, and syringe body adopts pmma material to make, and the push rod of microsyringe, needle stand, syringe needle are all stainless steel processing.
Ball-type switching valve 8 adopts hard steel to make, and has higher hardness and better wear-resisting, control stick can be made to carry out wide-angle and turn on a large scale, be easy to fixing.
Upper/lower guide 5, left and right rail 7, the three-dimensional guide assembly application precision three-dimensional guide rail technology that front and back guide rail 6 forms, by up and down, left and right, three track combination in front and back form, guide rail application helical teeth designs, gear parameter: modulus 0.3 mould, the number of teeth 18, helical angle 18 °, direction dextrorotation, tooth bar parameter: modulus 0.3 mould, helical angle 18 °, direction is left-handed, gear is arranged on dovetail groove center, tooth bar is fixed on dovetail, stripe shape gag is installed additional between dovetail groove and dovetail, outside dovetail groove, there are two to push up solid screw, the pressure of wheel and rack can be adjusted as required, therefore, it is possible to operating syringe carries out three-dimensional mobile accurately.
Magnetic-type anaesthetic room 9 periphery is around two iron rings, and insemination instrument base 11 studs with magnet, utilizes the suction of magnet to iron ring to fix queen bee anaesthetic room.
At four leg places of insemination instrument base 11, respectively with the screw that can realize height adjustment, the top of each screw is inlaid with antiskid glue cushion 12.
The present invention comprises the following steps in use,
Step one, drone adeagus is placed in polyethylene syringe needle 4 times, needle point is close to seminal fluid surface, pulls the screw rod of micro syringe 3 to suck seminal fluid;
Step 2, queen bee is put into magnetic-type anaesthetic room 9, by the lower end of magnetic-type anaesthetic room 9 access carbon dioxide;
Step 3, the afterbody of queen bee is placed in the upper end of magnetic-type anaesthetic room 9, adopts the back of the body hook that abdomen hook and back of the body hook control stick 2 that abdomen hook control stick 1 is arranged are arranged to be pulled open in the sting chamber of queen bee;
Step 4, adjustment upper/lower guide 5, front and back guide rail 6 and left and right rail 7, insert the introitus of queen bee by polyethylene needle point, the drone seminal fluid in syringe needle injects in the oviduct of queen bee by the screw rod promoting micro syringe 3, and queen bee artificial insemination completes.
Claims (4)
1. the artificial insemination instrument for queen bee with precision three-dimensional guide rail, it is characterized in that: comprise abdomen hook control stick (1), back of the body hook control stick (2), micro syringe (3), polyethylene syringe needle (4), upper/lower guide (5), front and back guide rail (6), left and right rail (7), spherical switching valve (8), magnetic-type anaesthetic room (9), column I (10), base (11), slipmat (12), column II (13) and spherical switching valve II (14), the outer ring of described magnetic-type anaesthetic room (9) is provided with iron ring, and be connected with base (11) by the magnet that base (11) side is arranged, described column I (10) and column II (13) are separately positioned on the left and right sides on base (11) top, and described abdomen hook control stick (1) is connected by the spherical switching valve I (8) be arranged on column I (10) with column I (10), described back of the body hook control stick (2) is connected by the spherical switching valve II (14) be arranged on column II (13) with column II (13), described upper/lower guide (5), front and back guide rail (6) and left and right rail (7) are combined into three-dimensional guide rail, three-dimensional guide rail is arranged on column II (13), and the design of upper/lower guide (5), front and back guide rail (6) and left and right rail (7) application helical teeth is interconnected, described polyethylene syringe needle (4) is connected with micro syringe (3), described micro syringe (3) is fixedly installed on upper/lower guide (5), described slipmat (12) is arranged on four legs of base (11) by height set screw.
2. with a using method for the artificial insemination instrument for queen bee of precision three-dimensional guide rail, it is characterized in that: comprise the following steps,
Step one, press with finger drone belly and force its adeagus to turn up, under drone adeagus is placed in polyethylene syringe needle, needle point is close to seminal fluid surface, pulls the screw rod of micro syringe to suck seminal fluid;
Step 2, queen bee is put into magnetic-type anaesthetic room (9), by the lower end of magnetic-type anaesthetic room (9) access carbon dioxide;
Step 3, the afterbody of queen bee is placed in the upper end of magnetic-type anaesthetic room (9), utilizes the back of the body hook on the abdomen hook on abdomen hook control stick (1) and back of the body hook control stick (2) to be pulled open in the sting chamber of queen bee;
Step 4, adjustment upper/lower guide (5), front and back guide rail (6) and left and right rail (7), the needle point of polyethylene syringe needle (4) is inserted the introitus of queen bee, drone seminal fluid in syringe needle injects in the oviduct of queen bee by the screw rod promoting micro syringe (3), and queen bee artificial insemination completes.
3. a kind of artificial insemination instrument for queen bee with precision three-dimensional guide rail according to claim 1 and 2 and using method thereof, is characterized in that: described polyethylene syringe needle (4) is transparent elastic syringe needle, and needle point is conical.
4. a kind of artificial insemination instrument for queen bee with precision three-dimensional guide rail according to claim 1 and 2 and using method thereof, is characterized in that: described upper/lower guide (5), front and back guide rail (6) and left and right rail (7) are connected by tooth bar, gear and dovetail groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510551609.2A CN105052839B (en) | 2015-09-01 | 2015-09-01 | A kind of artificial insemination instrument for queen bee and its application method with precision three-dimensional guide rail |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510551609.2A CN105052839B (en) | 2015-09-01 | 2015-09-01 | A kind of artificial insemination instrument for queen bee and its application method with precision three-dimensional guide rail |
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| Publication Number | Publication Date |
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| CN105052839A true CN105052839A (en) | 2015-11-18 |
| CN105052839B CN105052839B (en) | 2017-11-07 |
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| CN201510551609.2A Expired - Fee Related CN105052839B (en) | 2015-09-01 | 2015-09-01 | A kind of artificial insemination instrument for queen bee and its application method with precision three-dimensional guide rail |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106804538A (en) * | 2017-03-14 | 2017-06-09 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | A kind of queen bee mass detector and its detection method |
| CN107041341A (en) * | 2017-05-22 | 2017-08-15 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | A kind of Artificial Insemination For Honey Bees syringe needle drawing method and its drawing device |
| CN107079885A (en) * | 2017-06-14 | 2017-08-22 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Honeybee carries on the back web control device and Artificial Insemination For Honey Bees device |
| WO2020026999A1 (en) * | 2018-07-30 | 2020-02-06 | 国立大学法人京都大学 | Microsyringe unit |
| CN111802329A (en) * | 2020-07-31 | 2020-10-23 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Artificial insemination device |
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Cited By (10)
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| CN106804538A (en) * | 2017-03-14 | 2017-06-09 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | A kind of queen bee mass detector and its detection method |
| CN106804538B (en) * | 2017-03-14 | 2023-08-11 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Queen bee quality detector and detection method thereof |
| CN107041341A (en) * | 2017-05-22 | 2017-08-15 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | A kind of Artificial Insemination For Honey Bees syringe needle drawing method and its drawing device |
| CN107041341B (en) * | 2017-05-22 | 2023-10-31 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Drawing method and drawing device for artificial insemination needle head of bee |
| CN107079885A (en) * | 2017-06-14 | 2017-08-22 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Honeybee carries on the back web control device and Artificial Insemination For Honey Bees device |
| WO2020026999A1 (en) * | 2018-07-30 | 2020-02-06 | 国立大学法人京都大学 | Microsyringe unit |
| JPWO2020026999A1 (en) * | 2018-07-30 | 2021-08-02 | 国立大学法人京都大学 | Microsyringe unit |
| JP7418721B2 (en) | 2018-07-30 | 2024-01-22 | 国立大学法人京都大学 | Micro syringe unit |
| CN111802329A (en) * | 2020-07-31 | 2020-10-23 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Artificial insemination device |
| CN111802329B (en) * | 2020-07-31 | 2023-08-08 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Artificial insemination device |
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|---|---|
| CN105052839B (en) | 2017-11-07 |
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